Domain of unknown function (DUF4220)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 470 |
| AclWI | GGATC | 2 cut(s) | 320, 624 |
| AcsI | RAATTY | 2 cut(s) | 530, 743 |
| AcuI | CTGAAG | 1 cut(s) | 32 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 341 |
| AflIII | ACRYGT | 1 cut(s) | 725 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 145 |
| AjnI | CCWGG | 1 cut(s) | 473 |
| AluBI | AGCT | 4 cut(s) | 88, 133, 253, 349 |
| AluI | AGCT | 4 cut(s) | 88, 133, 253, 349 |
| Alw21I | GWGCWC | 2 cut(s) | 184, 680 |
| Alw26I | GTCTC | 1 cut(s) | 765 |
| Alw44I | GTGCAC | 1 cut(s) | 180 |
| AlwI | GGATC | 2 cut(s) | 320, 624 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 311 |
| Ama87I | CYCGRG | 1 cut(s) | 437 |
| AoxI | GGCC | 1 cut(s) | 735 |
| ApaLI | GTGCAC | 1 cut(s) | 180 |
| ApeKI | GCWGC | 2 cut(s) | 130, 870 |
| ApoI | RAATTY | 2 cut(s) | 530, 743 |
| AseI | ATTAAT | 1 cut(s) | 168 |
| AsuHPI | GGTGA | 1 cut(s) | 814 |
| AsuNHI | GCTAGC | 1 cut(s) | 782 |
| AvaI | CYCGRG | 1 cut(s) | 437 |
| BaeGI | GKGCMC | 1 cut(s) | 184 |
| BanI | GGYRCC | 1 cut(s) | 470 |
| BbsI | GAAGAC | 2 cut(s) | 20, 887 |
| Bbv12I | GWGCWC | 2 cut(s) | 184, 680 |
| BbvI | GCAGC | 2 cut(s) | 142, 882 |
| BccI | CCATC | 2 cut(s) | 114, 271 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 868, 902 |
| BciT130I | CCWGG | 1 cut(s) | 475 |
| BclI | TGATCA | 1 cut(s) | 535 |
| BcoDI | GTCTC | 1 cut(s) | 765 |
| BfaI | CTAG | 3 cut(s) | 35, 783, 875 |
| BisI | GCNGC | 2 cut(s) | 131, 871 |
| BlsI | GCNGC | 2 cut(s) | 132, 872 |
| Bme1390I | CCNGG | 1 cut(s) | 475 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 145 |
| BmeT110I | CYCGRG | 1 cut(s) | 437 |
| BmiI | GGNNCC | 2 cut(s) | 472, 716 |
| BmrFI | CCNGG | 1 cut(s) | 475 |
| BmsI | GCATC | 2 cut(s) | 170, 853 |
| BmtI | GCTAGC | 1 cut(s) | 786 |
| BpiI | GAAGAC | 2 cut(s) | 20, 887 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 169, 201 |
| BpuEI | CTTGAG | 3 cut(s) | 171, 535, 818 |
| BsaBI | GATNNNNATC | 1 cut(s) | 387 |
| BsaJI | CCNNGG | 1 cut(s) | 732 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 341 |
| Bse3DI | GCAATG | 1 cut(s) | 884 |
| Bse8I | GATNNNNATC | 1 cut(s) | 387 |
| BseBI | CCWGG | 1 cut(s) | 475 |
| BseDI | CCNNGG | 1 cut(s) | 732 |
| BseGI | GGATG | 1 cut(s) | 263 |
| BseJI | GATNNNNATC | 1 cut(s) | 387 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 341 |
| BseMI | GCAATG | 1 cut(s) | 884 |
| BseMII | CTCAG | 1 cut(s) | 185 |
| BseRI | GAGGAG | 1 cut(s) | 670 |
| BseSI | GKGCMC | 1 cut(s) | 184 |
| BseXI | GCAGC | 2 cut(s) | 142, 882 |
| BsgI | GTGCAG | 1 cut(s) | 149 |
| BshFI | GGCC | 1 cut(s) | 737 |
| BshNI | GGYRCC | 1 cut(s) | 470 |
| BsiHKAI | GWGCWC | 2 cut(s) | 184, 680 |
| BsiHKCI | CYCGRG | 1 cut(s) | 437 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 341 |
| BsmAI | GTCTC | 1 cut(s) | 765 |
| BsnI | GGCC | 1 cut(s) | 737 |
| BsoBI | CYCGRG | 1 cut(s) | 437 |
| Bsp1286I | GDGCHC | 2 cut(s) | 184, 680 |
| Bsp143I | GATC | 3 cut(s) | 325, 535, 616 |
| BspANI | GGCC | 1 cut(s) | 737 |
| BspCNI | CTCAG | 1 cut(s) | 186 |
| BspLI | GGNNCC | 2 cut(s) | 472, 716 |
| BspOI | GCTAGC | 1 cut(s) | 786 |
| BspPI | GGATC | 2 cut(s) | 320, 624 |
| BspT107I | GGYRCC | 1 cut(s) | 470 |
| BsrDI | GCAATG | 1 cut(s) | 884 |
| BssECI | CCNNGG | 1 cut(s) | 732 |
| BssMI | GATC | 3 cut(s) | 325, 535, 616 |
| BssT1I | CCWWGG | 1 cut(s) | 732 |
| Bst2UI | CCWGG | 1 cut(s) | 475 |
| Bst4CI | ACNGT | 1 cut(s) | 228 |
| Bst6I | CTCTTC | 2 cut(s) | 170, 587 |
| BstC8I | GCNNGC | 3 cut(s) | 96, 784, 868 |
| BstDEI | CTNAG | 1 cut(s) | 194 |
| BstF5I | GGATG | 1 cut(s) | 263 |
| BstKTI | GATC | 3 cut(s) | 328, 538, 619 |
| BstMAI | GTCTC | 1 cut(s) | 765 |
| BstMBI | GATC | 3 cut(s) | 325, 535, 616 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 100, 788 |
| BstNI | CCWGG | 1 cut(s) | 475 |
| BstNSI | RCATGY | 2 cut(s) | 406, 729 |
| BstSCI | CCNGG | 1 cut(s) | 473 |
| BstSLI | GKGCMC | 1 cut(s) | 184 |
| BstV1I | GCAGC | 2 cut(s) | 142, 882 |
| BstV2I | GAAGAC | 2 cut(s) | 20, 887 |
| BsuRI | GGCC | 1 cut(s) | 737 |
| BtsCI | GGATG | 1 cut(s) | 263 |
| Cac8I | GCNNGC | 3 cut(s) | 96, 784, 868 |
| CaiI | CAGNNNCTG | 1 cut(s) | 311 |
| CseI | GACGC | 1 cut(s) | 100 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 589, 624 |
| CviAII | CATG | 3 cut(s) | 403, 500, 726 |
| DdeI | CTNAG | 1 cut(s) | 194 |
| DpnI | GATC | 3 cut(s) | 327, 537, 618 |
| DpnII | GATC | 3 cut(s) | 325, 535, 616 |
| DriI | GACNNNNNGTC | 1 cut(s) | 145 |
| Eam1104I | CTCTTC | 2 cut(s) | 170, 587 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 145 |
| EarI | CTCTTC | 2 cut(s) | 170, 587 |
| Eco130I | CCWWGG | 1 cut(s) | 732 |
| Eco57I | CTGAAG | 1 cut(s) | 32 |
| Eco88I | CYCGRG | 1 cut(s) | 437 |
| EcoRI | GAATTC | 1 cut(s) | 743 |
| EcoRII | CCWGG | 1 cut(s) | 473 |
| EcoT14I | CCWWGG | 1 cut(s) | 732 |
| ErhI | CCWWGG | 1 cut(s) | 732 |
| FaeI | CATG | 3 cut(s) | 406, 503, 729 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 780, 812 |
| FatI | CATG | 3 cut(s) | 402, 499, 725 |
| FbaI | TGATCA | 1 cut(s) | 535 |
| Fnu4HI | GCNGC | 2 cut(s) | 131, 871 |
| FokI | GGATG | 1 cut(s) | 250 |
| Fsp4HI | GCNGC | 2 cut(s) | 131, 871 |
| FspBI | CTAG | 3 cut(s) | 35, 783, 875 |
| GluI | GCNGC | 2 cut(s) | 131, 871 |
| HaeIII | GGCC | 1 cut(s) | 737 |
| HgaI | GACGC | 1 cut(s) | 100 |
| Hin1II | CATG | 3 cut(s) | 406, 503, 729 |
| HindIII | AAGCTT | 2 cut(s) | 251, 347 |
| HinfI | GANTC | 3 cut(s) | 46, 146, 478 |
| HphI | GGTGA | 1 cut(s) | 814 |
| Hpy166II | GTNNAC | 2 cut(s) | 182, 231 |
| Hpy188I | TCNGA | 3 cut(s) | 195, 382, 649 |
| Hpy188III | TCNNGA | 4 cut(s) | 35, 246, 312, 482 |
| Hpy8I | GTNNAC | 2 cut(s) | 182, 231 |
| HpyAV | CCTTC | 2 cut(s) | 71, 554 |
| HpyCH4III | ACNGT | 1 cut(s) | 228 |
| HpyCH4IV | ACGT | 1 cut(s) | 457 |
| HpyCH4V | TGCA | 7 cut(s) | 130, 182, 447, 563, 765, 870, 889 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 100, 788 |
| HpyF3I | CTNAG | 1 cut(s) | 194 |
| HpySE526I | ACGT | 1 cut(s) | 457 |
| Hsp92II | CATG | 3 cut(s) | 406, 503, 729 |
| Ksp22I | TGATCA | 1 cut(s) | 535 |
| Kzo9I | GATC | 3 cut(s) | 325, 535, 616 |
| LmnI | GCTCC | 1 cut(s) | 683 |
| Lsp1109I | GCAGC | 2 cut(s) | 142, 882 |
| LweI | GCATC | 2 cut(s) | 170, 853 |
| MaeI | CTAG | 3 cut(s) | 35, 783, 875 |
| MaeII | ACGT | 1 cut(s) | 457 |
| MaeIII | GTNAC | 1 cut(s) | 52 |
| MalI | GATC | 3 cut(s) | 327, 537, 618 |
| MboI | GATC | 3 cut(s) | 325, 535, 616 |
| MboII | GAAGA | 9 cut(s) | 25, 187, 536, 539, 539, 604, 656, 764, 892 |
| MfeI | CAATTG | 1 cut(s) | 890 |
| MhlI | GDGCHC | 2 cut(s) | 184, 680 |
| MluCI | AATT | 6 cut(s) | 315, 452, 530, 743, 828, 890 |
| MlyI | GAGTC | 1 cut(s) | 155 |
| MnlI | CCTC | 9 cut(s) | 195, 216, 227, 319, 433, 607, 691, 793, 828 |
| MseI | TTAA | 3 cut(s) | 168, 321, 831 |
| MslI | CAYNNNNRTG | 1 cut(s) | 468 |
| MspA1I | CMGCKG | 2 cut(s) | 88, 133 |
| MspR9I | CCNGG | 1 cut(s) | 475 |
| MunI | CAATTG | 1 cut(s) | 890 |
| MvaI | CCWGG | 1 cut(s) | 475 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 100, 788 |
| NdeII | GATC | 3 cut(s) | 325, 535, 616 |
| NheI | GCTAGC | 1 cut(s) | 782 |
| NlaIII | CATG | 3 cut(s) | 406, 503, 729 |
| NlaIV | GGNNCC | 2 cut(s) | 472, 716 |
| NmuCI | GTSAC | 1 cut(s) | 52 |
| NspI | RCATGY | 2 cut(s) | 406, 729 |
| PaeR7I | CTCGAG | 1 cut(s) | 437 |
| PciI | ACATGT | 1 cut(s) | 725 |
| PfeI | GAWTC | 2 cut(s) | 46, 478 |
| PkrI | GCNGC | 2 cut(s) | 132, 872 |
| PleI | GAGTC | 1 cut(s) | 154 |
| PpsI | GAGTC | 1 cut(s) | 154 |
| PscI | ACATGT | 1 cut(s) | 725 |
| PshBI | ATTAAT | 1 cut(s) | 168 |
| Psp6I | CCWGG | 1 cut(s) | 473 |
| PspGI | CCWGG | 1 cut(s) | 473 |
| PspN4I | GGNNCC | 2 cut(s) | 472, 716 |
| PspXI | VCTCGAGB | 1 cut(s) | 437 |
| PstNI | CAGNNNCTG | 1 cut(s) | 311 |
| PvuII | CAGCTG | 2 cut(s) | 88, 133 |
| RseI | CAYNNNNRTG | 1 cut(s) | 468 |
| SaqAI | TTAA | 3 cut(s) | 168, 321, 831 |
| SatI | GCNGC | 2 cut(s) | 131, 871 |
| Sau3AI | GATC | 3 cut(s) | 325, 535, 616 |
| SchI | GAGTC | 1 cut(s) | 155 |
| ScrFI | CCNGG | 1 cut(s) | 475 |
| SduI | GDGCHC | 2 cut(s) | 184, 680 |
| SfaNI | GCATC | 2 cut(s) | 170, 853 |
| Sfr274I | CTCGAG | 1 cut(s) | 437 |
| SlaI | CTCGAG | 1 cut(s) | 437 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 468 |
| SmlI | CTYRAG | 4 cut(s) | 186, 437, 514, 797 |
| SmoI | CTYRAG | 4 cut(s) | 186, 437, 514, 797 |
| Sse9I | AATT | 6 cut(s) | 315, 452, 530, 743, 828, 890 |
| SspMI | CTAG | 3 cut(s) | 35, 783, 875 |
| StyD4I | CCNGG | 1 cut(s) | 473 |
| StyI | CCWWGG | 1 cut(s) | 732 |
| TaaI | ACNGT | 1 cut(s) | 228 |
| TaiI | ACGT | 1 cut(s) | 460 |
| TaqI | TCGA | 2 cut(s) | 438, 612 |
| TasI | AATT | 6 cut(s) | 315, 452, 530, 743, 828, 890 |
| TfiI | GAWTC | 2 cut(s) | 46, 478 |
| Tru1I | TTAA | 3 cut(s) | 168, 321, 831 |
| Tru9I | TTAA | 3 cut(s) | 168, 321, 831 |
| TseFI | GTSAC | 1 cut(s) | 52 |
| TseI | GCWGC | 2 cut(s) | 130, 870 |
| Tsp45I | GTSAC | 1 cut(s) | 52 |
| TspDTI | ATGAA | 2 cut(s) | 281, 605 |
| VneI | GTGCAC | 1 cut(s) | 180 |
| VspI | ATTAAT | 1 cut(s) | 168 |
| XapI | RAATTY | 2 cut(s) | 530, 743 |
| XbaI | TCTAGA | 1 cut(s) | 34 |
| XceI | RCATGY | 2 cut(s) | 406, 729 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 506 |
| XhoI | CTCGAG | 1 cut(s) | 437 |
| XspI | CTAG | 3 cut(s) | 35, 783, 875 |