Prupe.3G041500_v2.0.a1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000657
Physical Location & Seq
Reverse (-)
2962806 .. 2967843
5038 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G041500.1

Sequence Viewer

Length: 4080 bp
ATGGAAACGATCACAGAATGGTTCATTGCAGGAATACCTGGTGCTCAGCCATTGGGATCCTCAACAACGGAGACGGATTGGAAAGCTATGGGACATTATATAGAGAAAGATGATGCTGATCAGGAGTGGACGGCTTCCAATGCTGAAGAACTGGAAAAATATGGTCTTTATCGTTATCATTTTGATAGAAGTCTGGATAATTTGGCCAACCAGAAGGAGGTAACAACAACCAAGCTGGCATTCCTTGATTCTATTCTCCATGATGCTCATGTTTATATCTTAGTTGTTGTAGACCTTATACTCCTTTATGTAAACCATACGTCACTGTATCGGGTGCTACTGATTTCGACGCTTGCAAACAGTTATGTGCTCGTATGGGTGCCCTTCTGTGCCTCTCTAATCACCCTATTGTTCCTCTACTTGTATATCAACCACTTTCCGTTTCTGTTCTTGGAGGAAGACATCAAATGGATTCGAAAAGAGTCACGGTTACTAAGTGCTATTTCGGAGGATGCAGAAGAAGTTAAGCGTTTATGCTTTAAAATAGACGCCAGGGCACATCAAATCTGGGACTCTATTTTCCCTAGTAAAGGAACCTTTCCTGGACTAGAGGAAACTGAGGTACAACTGGTGTACAAAACCGTAACCGTGGCTGGACTAGATGAAATTGAGGCAAAATGGGTGGACAAAATTGCATCCACTGTAAAAGAATCACAACGTTTAGTTAAAACGTTTGAGGAAAAGAGACGAGACTCCCACAGACTTGGAATATTTTTTGGGATGACTACAAAGAATTTTGAGCGGACAGATAAGCTTTTCAGTAAAACTAAGCAGGTAAAGGAGGAAATAAACAGCAGCATTGTGATGAAGAAGGAAAGTGGGATTAACATATGTGAATCCCTGGAGAGATTAAAGTCTAATGCTAGAAGGCTGCTGGAAAGACCTTTTGATGAACATGAAAAAAGATGGGTAAACTCCGGAGAACGAGTTGAATCTGCAGCTGCATCGGCTTTCTCTACTGTAGAGAAAATAAATAGTCTAATCATTCAAAACCCAGAGTTAATGCCAGACAGGAGTAGAATTGGAGAGCAACTACAGTCAACAAATCTGCAATTGCAGCTGCTACATCCTTTTCTGAAAGATATACAAGGAATTAATGAATTTGAGAGCGCAATTGAGAAGGCCTGGGTGGAGGAAGTGGAAGAGATCATTGATGAAGCACATCCTGCCATTGAAAACTTCTTACAGACACCATCCAATAGATTTGGGTGGCTATCATATATCAGCAGTTGGAGGGCAAGGAGAAAGCTCGAGGGGGACCTAAGGTGCATCAACGTTGGGTTCACACAAGCTTTAGAAAGGAAGGGCAGGTACGGCTTCAAATTTATAAGAAGAATTTCTAATTCATCAAAGATTGTCTATCAATCCCCAGATCAGGCCGCAGATGAGAATAGTGTTTCATCTGTAATAGACAGGATGCGCAACTACCTCAAAGAGGAGCCAAATTTATTCCGTGAGGTATTTTTTGATGTCAAATTGCTTAGCAATGAACTGGAAATCATGCATAAACTTGTAGGCGGTGCAGGAATGATTGGAAGGAGATATAATTCCAGAGTCGCTTGGTTGGAGCAAGTGAGGAAAATTGTTCAAAGTGCAGATGAATCTGTGGTAACCTTTATGAAGATGAATTCAGGTTTAGAAGCAGACAGAGATGATAAAAAGCTTAAGAATTCTGGAAGAAAGCTATCCCTTGAAATCAACCAGATTAATCAGATTATCAATCTTTTCTTGAGATGCATAAAAGCATTCAGCATTGAATCAAGGGAAGACTTAAGCTCAGTGGTCGGTTTGGAAGAAGACATAGATGCAGTGGTGTCAAGACTGAGAACCAACAATGAACACTGCTCTTTTGTTTCCATAGTGGGGATTGAGGGCATAGGCAAGACAACACTGGCAAATAAAATTTATAACCACGGAGTCATTGTGGACCATTTTCCTTGTCGTGCATGGGTTTCTCTACCTCACGACTACTCCTACAACAATAAACTGCCACTCTTGAAATACGTTGCAAAGCAGGTCTTGAGCTCTCTTAAGTCAGAAGGGGACCAAGAGATTAATCCCGATGCATCCAACAGGATCGACGAGGCACATGATATTTTAAAAAAGAGTCGGTACCTTTTAGTTCTTGACAACATCTCCACAATGTACGAGTGGAGTACTCTGAAAGCAGCATTTCCATTAAGTACATCCAATGGAAGCAGAATTCTGCTCACTACACGTAACCCTGAAGTGGCTTCACAAGTGGATAGCGACAGCCCTCCCCACCACCTTCGACGGCTAACCAAAGAAGAGAGCTGGCAGTTGTTTAGCCAGGTGGTGCACATCCCTCCGAAAGGGAAAATGCTAGCAAAAGAAATTTTGAGTAAATGTGAGGGTTTACCACTAGCCATTGTACGTCTAATGTCAGGGAAAGATGAGACTACTGCTCAGGAGTTGAAAAAGGTGATTCAATACATCAAACAGGATGATGATACATCGTCGTTCTGTACCTACACCAAAGAGTGCATAAATAAGTTTCCTGATCATCTTACTGATTGTCTTTCTTGCTTCAAACTCTTCCCCAAGGACTATAAAATTCCAGTGAGGAGATTATTCGCTTTGTGGATTGCTGAAGAGTTGGTAGAAGTAAGTGACCACAAGACAGAAAATCATGAATCTGACCCAAAGAAAAAGGCTGAAACTTACGAAGAAGCAGCGGATGAGCATCTATCTGCATTGATCGATCGTGACATCGTTCATGTAGTTGAAAGAAAGCTTAATGGGAAAGTCAAGACATGTTGCTTGAACAAAGATTTACAAGAAGTGATCAGGTCACAAGCCATGAGGTTAGATCATAGGCGCCTTGCTGATCATGTAAACTGTAATGATCCAAGTTTTGATCTTATTCATGGTGAAAGCTCCAATTTTTCAAGATCTTACACAGATCTCATTTCCATTCTATCCTTTGATTCCCAAGAAGGATATAAACCTGGAGAAGACATAGGCAACTTCCTACGAAGGGGCATTGCAGGTGGTTATTTCCTTCAGCTAAAGTTTCTTGATCTTGAACGTGTATTCAGACCTGAATTGCCCAACACCATAGTAAAATTAAGCAATTTGAGGTATCTAGGCTTGAGGTGGACTTCCCTTGAATCAATCCCAGATTCCATTGGTGAATTGGTGAACCTCCAAACCTTGGATGTGAAGCACACATATGTTCGCACTCTCCCCCGTTCAATATGGAAACTGCTCAAACTTCGACACCTATACTTAAGTCAAAGGTATCGAAGTAAATTTATGCGTCACCAAGGTCTCAATTCTCTCAAGAATCTCCGGACATTATGGGGTGTATTTGTGGATAAGCATAGTCCCTTAAAGGATGGTTTGGATAAATTTATAAATCTCAGAAAATTGGGATTGGCATTCCAGTTAGAGAAGGAAGAGCAAAAAGTATTAGCAGAAAGGATCGTAAAGCTGAGCTATCTCAAGTCCTTAAGAATGAGATCAATTGATGAAATGGGTGAACCCTGTCCTCTGATGTTGGAGTCTTTGTCAGGCCTTGAGAATCTCTCCAGCTTAAATTTGTTTGGGATGCTAGAAAATCCTTCCATCATAGCTGGATTTCCTAAAAACCTCATTGATCTTACTTTGTCAGCCTCTTCATTTTCAGAAGATCCAATGCCCAAGTTAGAGAAGCTTTCCAACCTTCAATCACTATGTTTCTACTCCAACTCTTATACCGGTACAAAGATGGTTTGCTCCACCTGGGGATTCCAAAAGCTTGTAGTGTTAAAGTTGTGGAAGCTAGAACGGCTAGAAGATTGGGATGTGGAGGAAAAGGCAATGCAAAACATCAGGGAGTTGGAGATCAGATCCTGCAATAAGCTGAAGGTCCCTACTGGCTTAAGGCACTTGAAGACTCTCATTGAATTGAAGCTAATTAATATGCCAAAACAATTTTCTGCAACCATTGAAAAAACTAATGTGGACATTTGGGGTGACATTGCCCACTCCCCAACAATCATTACTGATAGCTGGTAG

Protein Analysis

1360

Amino Acids

156.36

Weight (kDa)

6.68

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34770
malus_domestica MD17G1025700.v1.1
prunus_persica Prupe.3G040600_v2.0.a1 Prupe.3G040800_v2.0.a1 Prupe.3G041000_v2.0.a1 Prupe.3G041100_v2.0.a1 Prupe.3G041500_v2.0.a1 Prupe.4G109400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037700_v2.0.a1
pyrus_communis pycom09g10040 pycom17g16000
rosa_chinensis RchiOBHm_Chr2g0145591 RchiOBHm_Chr2g0145611
rosa_laevigata RLG00000020146 RLG00000020149 RLG00000020151 RLG00000020154
rosa_multiflora Rmu_co7986250.1_g000001 Rmu_co8305779.1_g000001 Rmu_sc0002718.1_g000011 Rmu_sc0002835.1_g000009 Rmu_sc0002835.1_g000016 Rmu_sc0003046.1_g000007 Rmu_sc0003046.1_g000010 Rmu_sc0004445.1_g000006 Rmu_sc0004810.1_g000002 Rmu_sc0006477.1_g000008 Rmu_sc0014329.1_g000001 Rmu_sc0020976.1_g000003 Rmu_sc0021168.1_g000001 Rmu_sc0022199.1_g000001 Rmu_sc0036991.1_g000001 Rmu_sc0041497.1_g000003
rosa_roxburghii Rroxscaffold_152G00434560 Rroxscaffold_152G00434570 Rroxscaffold_152G00434600 Rroxscaffold_152G00434610 Rroxscaffold_2G00100630 Rroxscaffold_2G00100640 Rroxscaffold_2G00100660 Rroxscaffold_2G00100670 Rroxscaffold_2G00100680 Rroxscaffold_2G00100690
rosa_rugosa Rorug02G0392300 Rorug02G0392400 Rorug02G0392400
rosa_samantha Rh2AG445700 Rh2AG445800 Rh2AG446000 Rh2AG446100 Rh2AG446300 Rh2AG446600 Rh2BG457400 Rh2BG457500 Rh2BG457600 Rh2BG457900 Rh2BG458300 Rh2CG432300 Rh2CG432400 Rh2CG432500 Rh2CG432700 Rh2CG432900 Rh2CG433000 Rh2CG433300 Rh2DG467100 Rh2DG467200 Rh2DG467300 Rh2DG467600 Rh2DG467900 Rh3DG194100
rosa_wichuraiana Rw2G036420 Rw2G036450 Rw2G036470 Rw3G014900 Rw3G014920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1388, 1968, 3437
AarI CACCTGC 1 cut(s) 3059
Acc16I TGCGCA 1 cut(s) 1481
Acc36I ACCTGC 4 cut(s) 823, 1359, 2065, 3059
Acc65I GGTACC 1 cut(s) 2172
AccB1I GGYRCC 3 cut(s) 379, 2172, 2898
AccB7I CCANNNNNTGG 1 cut(s) 3235
AccBSI CCGCTC 1 cut(s) 802
AccI GTMKAC 1 cut(s) 291
AccIII TCCGGA 2 cut(s) 977, 3372
AciI CCGC 4 cut(s) 802, 1440, 1578, 2756
AclI AACGTT 3 cut(s) 718, 731, 1335
AclWI GGATC 7 cut(s) 51, 64, 2144, 2921, 3512, 3707, 3906
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 5 cut(s) 165, 2307, 2691, 3068, 3947
AcyI GRCGYC 2 cut(s) 549, 2899
AfiI CCNNNNNNNGG 9 cut(s) 217, 590, 1435, 1495, 1923, 2290, 2393, 3058, 3235
AflII CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
AflIII ACRYGT 3 cut(s) 2276, 2834, 3109
AgeI ACCGGT 1 cut(s) 3779
AjnI CCWGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
AjuI GAANNNNNNNTTGG 4 cut(s) 224, 256, 3982, 4014
Alw21I GWGCWC 4 cut(s) 46, 372, 2087, 2382
Alw26I GTCTC 5 cut(s) 65, 739, 744, 2471, 3356
Alw44I GTGCAC 1 cut(s) 2378
AlwI GGATC 7 cut(s) 51, 64, 2144, 2921, 3512, 3707, 3906
AlwNI CAGNNNCTG 1 cut(s) 3915
Ama87I CYCGRG 1 cut(s) 1310
Aor13HI TCCGGA 2 cut(s) 977, 3372
AoxI GGCC 4 cut(s) 204, 1182, 1437, 3595
ApaLI GTGCAC 1 cut(s) 2378
ApeKI GCWGC 8 cut(s) 855, 931, 998, 1001, 1117, 1120, 2228, 2753
AseI ATTAAT 4 cut(s) 1155, 1767, 2115, 3981
AsiGI ACCGGT 1 cut(s) 3779
Asp700I GAANNNNTTC 1 cut(s) 1396
Asp718I GGTACC 1 cut(s) 2172
AspLEI GCGC 3 cut(s) 1172, 1482, 2901
AspS9I GGNCC 4 cut(s) 1318, 1987, 2104, 3931
AsuHPI GGTGA 8 cut(s) 394, 2515, 2963, 3224, 3232, 3335, 3572, 4049
AsuII TTCGAA 1 cut(s) 475
AsuNHI GCTAGC 1 cut(s) 2404
AvaI CYCGRG 1 cut(s) 1310
AvaII GGWCC 4 cut(s) 1318, 1987, 2104, 3931
AxyI CCTNAGG 1 cut(s) 1322
BaeGI GKGCMC 3 cut(s) 384, 559, 2382
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 56
BanI GGYRCC 3 cut(s) 379, 2172, 2898
BanII GRGCYC 1 cut(s) 2087
BarI GAAGNNNNNNTAC 2 cut(s) 1355, 1387
BbsI GAAGAC 5 cut(s) 465, 1833, 1863, 3042, 3962
Bbv12I GWGCWC 4 cut(s) 46, 372, 2087, 2382
BbvI GCAGC 8 cut(s) 867, 918, 988, 1010, 1107, 1129, 2240, 2765
BccI CCATC 5 cut(s) 960, 1261, 3413, 3656, 3784
BceAI ACGGC 4 cut(s) 147, 1390, 2351, 3866
BcgI CGANNNNNNTGC 2 cut(s) 987, 1021
BciT130I CCWGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
BclI TGATCA 4 cut(s) 118, 2581, 2865, 2908
BcoDI GTCTC 5 cut(s) 65, 739, 744, 2471, 3356
BfmI CTRYAG 3 cut(s) 996, 1020, 1094
BfoI RGCGCY 1 cut(s) 2902
BfrI CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
BfuAI ACCTGC 4 cut(s) 823, 1359, 2065, 3059
BglII AGATCT 2 cut(s) 2972, 2983
BisI GCNGC 9 cut(s) 856, 932, 999, 1002, 1118, 1121, 1440, 2229, 2754
BlpI GCTNAGC 3 cut(s) 45, 1541, 3515
BlsI GCNGC 9 cut(s) 857, 933, 1000, 1003, 1119, 1122, 1441, 2230, 2755
BmcAI AGTACT 1 cut(s) 2218
Bme1390I CCNGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
Bme18I GGWCC 4 cut(s) 1318, 1987, 2104, 3931
BmeT110I CYCGRG 1 cut(s) 1310
BmgT120I GGNCC 4 cut(s) 1318, 1987, 2104, 3931
BmiI GGNNCC 9 cut(s) 58, 381, 595, 1319, 1500, 2105, 2174, 2900, 3933
BmrFI CCNGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
BmtI GCTAGC 1 cut(s) 2408
BpiI GAAGAC 5 cut(s) 465, 1833, 1863, 3042, 3962
BplI GAGNNNNNCTC 2 cut(s) 3593, 3625
BpmI CTGGAG 3 cut(s) 923, 3051, 3595
Bpu10I CCTNAGC 1 cut(s) 2487
Bpu1102I GCTNAGC 3 cut(s) 45, 1541, 3515
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 6 cut(s) 1810, 2101, 3193, 3347, 3509, 3620
Bsa29I ATCGAT 1 cut(s) 2781
BsaAI YACGTR 1 cut(s) 2279
BsaBI GATNNNNATC 2 cut(s) 117, 2763
BsaHI GRCGYC 2 cut(s) 549, 2899
BsaI GGTCTC 1 cut(s) 3356
BsaJI CCNNGG 9 cut(s) 552, 648, 900, 1185, 1972, 2622, 3234, 3346, 3804
BsaWI WCCGGW 3 cut(s) 977, 3372, 3779
Bsc4I CCNNNNNNNGG 9 cut(s) 217, 590, 1435, 1495, 1923, 2290, 2393, 3058, 3235
Bse118I RCCGGY 1 cut(s) 3779
Bse1I ACTGG 7 cut(s) 156, 633, 1557, 1956, 2639, 3466, 3943
Bse21I CCTNAGG 1 cut(s) 1322
Bse3DI GCAATG 5 cut(s) 24, 1552, 3063, 3888, 4041
Bse8I GATNNNNATC 2 cut(s) 117, 2763
BseAI TCCGGA 2 cut(s) 977, 3372
BseBI CCWGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
BseCI ATCGAT 1 cut(s) 2781
BseDI CCNNGG 9 cut(s) 552, 648, 900, 1185, 1972, 2622, 3234, 3346, 3804
BseJI GATNNNNATC 2 cut(s) 117, 2763
BseLI CCNNNNNNNGG 9 cut(s) 217, 590, 1435, 1495, 1923, 2290, 2393, 3058, 3235
BseMI GCAATG 5 cut(s) 24, 1552, 3063, 3888, 4041
BseMII CTCAG 7 cut(s) 59, 609, 1851, 1874, 2501, 3457, 3506
BseNI ACTGG 7 cut(s) 156, 633, 1557, 1956, 2639, 3466, 3943
BseRI GAGGAG 2 cut(s) 1511, 2659
BseSI GKGCMC 3 cut(s) 384, 559, 2382
BseXI GCAGC 8 cut(s) 867, 918, 988, 1010, 1107, 1129, 2240, 2765
BsgI GTGCAG 2 cut(s) 1602, 1674
Bsh1285I CGRYCG 1 cut(s) 2785
BshFI GGCC 4 cut(s) 206, 1184, 1439, 3597
BshNI GGYRCC 3 cut(s) 379, 2172, 2898
BshTI ACCGGT 1 cut(s) 3779
BshVI ATCGAT 1 cut(s) 2781
BsiEI CGRYCG 1 cut(s) 2785
BsiHKAI GWGCWC 4 cut(s) 46, 372, 2087, 2382
BsiHKCI CYCGRG 1 cut(s) 1310
BsiSI CCGG 3 cut(s) 978, 3373, 3780
BslFI GGGAC 6 cut(s) 105, 584, 1331, 2117, 3393, 3917
BslI CCNNNNNNNGG 9 cut(s) 217, 590, 1435, 1495, 1923, 2290, 2393, 3058, 3235
BsmAI GTCTC 5 cut(s) 65, 739, 744, 2471, 3356
BsmBI CGTCTC 2 cut(s) 65, 739
BsmFI GGGAC 6 cut(s) 105, 584, 1331, 2117, 3393, 3917
BsmI GAATGC 3 cut(s) 239, 1805, 3461
BsnI GGCC 4 cut(s) 206, 1184, 1439, 3597
Bso31I GGTCTC 1 cut(s) 3356
BsoBI CYCGRG 1 cut(s) 1310
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 6 cut(s) 46, 372, 384, 559, 2087, 2382
Bsp13I TCCGGA 2 cut(s) 977, 3372
Bsp1407I TGTACA 1 cut(s) 633
Bsp1720I GCTNAGC 3 cut(s) 45, 1541, 3515
BspACI CCGC 4 cut(s) 802, 1440, 1578, 2756
BspANI GGCC 4 cut(s) 206, 1184, 1439, 3597
BspCNI CTCAG 7 cut(s) 58, 610, 1850, 1875, 2500, 3456, 3507
BspDI ATCGAT 1 cut(s) 2781
BspEI TCCGGA 2 cut(s) 977, 3372
BspHI TCATGA 1 cut(s) 2710
BspLI GGNNCC 9 cut(s) 58, 381, 595, 1319, 1500, 2105, 2174, 2900, 3933
BspMAI CTGCAG 1 cut(s) 1000
BspMI ACCTGC 4 cut(s) 823, 1359, 2065, 3059
BspOI GCTAGC 1 cut(s) 2408
BspPI GGATC 7 cut(s) 51, 64, 2144, 2921, 3512, 3707, 3906
BspQI GCTCTTC 1 cut(s) 3474
BspT104I TTCGAA 1 cut(s) 475
BspT107I GGYRCC 3 cut(s) 379, 2172, 2898
BspTI CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
BspTNI GGTCTC 1 cut(s) 3356
BsrBI CCGCTC 1 cut(s) 802
BsrDI GCAATG 5 cut(s) 24, 1552, 3063, 3888, 4041
BsrFI RCCGGY 1 cut(s) 3779
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 7 cut(s) 156, 633, 1557, 1956, 2639, 3466, 3943
BssAI RCCGGY 1 cut(s) 3779
BssECI CCNNGG 9 cut(s) 552, 648, 900, 1185, 1972, 2622, 3234, 3346, 3804
BssNI GRCGYC 2 cut(s) 549, 2899
BssT1I CCWWGG 3 cut(s) 2622, 3234, 3346
Bst2UI CCWGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
Bst4CI ACNGT 9 cut(s) 327, 362, 489, 643, 649, 703, 1021, 1098, 2921
Bst6I CTCTTC 6 cut(s) 1197, 2343, 2621, 2667, 3474, 3703
BstACI GRCGYC 2 cut(s) 549, 2899
BstAFI CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
BstAPI GCANNNNNTGC 1 cut(s) 1226
BstAUI TGTACA 1 cut(s) 633
BstBAI YACGTR 1 cut(s) 2279
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 4 cut(s) 237, 354, 2357, 2406
BstDSI CCRYGG 2 cut(s) 648, 1972
BstEII GGTNACC 1 cut(s) 1669
BstENI CCTNNNNNAGG 3 cut(s) 588, 1493, 2391
BstH2I RGCGCY 1 cut(s) 2902
BstHHI GCGC 3 cut(s) 1172, 1482, 2901
BstMAI GTCTC 5 cut(s) 65, 739, 744, 2471, 3356
BstMCI CGRYCG 1 cut(s) 2785
BstMWI GCNNNNNNNGC 6 cut(s) 140, 1007, 1117, 1226, 1374, 3850
BstNI CCWGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
BstNSI RCATGY 1 cut(s) 2838
BstPI GGTNACC 1 cut(s) 1669
BstSCI CCNGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
BstSFI CTRYAG 3 cut(s) 996, 1020, 1094
BstSLI GKGCMC 3 cut(s) 384, 559, 2382
BstV1I GCAGC 8 cut(s) 867, 918, 988, 1010, 1107, 1129, 2240, 2765
BstV2I GAAGAC 5 cut(s) 465, 1833, 1863, 3042, 3962
BstX2I RGATCY 5 cut(s) 56, 2972, 2983, 3712, 3911
BstXI CCANNNNNNTGG 1 cut(s) 764
BstYI RGATCY 5 cut(s) 56, 2972, 2983, 3712, 3911
Bsu15I ATCGAT 1 cut(s) 2781
Bsu36I CCTNAGG 1 cut(s) 1322
BsuRI GGCC 4 cut(s) 206, 1184, 1439, 3597
BsuTUI ATCGAT 1 cut(s) 2781
BtgI CCRYGG 2 cut(s) 648, 1972
BtsI GCAGTG 2 cut(s) 1875, 1900
BtsIMutI CAGTG 7 cut(s) 323, 699, 1845, 1875, 1900, 1949, 2646
BveI ACCTGC 4 cut(s) 823, 1359, 2065, 3059
Cac8I GCNNGC 4 cut(s) 237, 354, 2357, 2406
CaiI CAGNNNCTG 1 cut(s) 3915
CciI TCATGA 1 cut(s) 2710
CfoI GCGC 3 cut(s) 1172, 1482, 2901
Cfr10I RCCGGY 1 cut(s) 3779
Cfr13I GGNCC 4 cut(s) 1318, 1987, 2104, 3931
ClaI ATCGAT 1 cut(s) 2781
CseI GACGC 3 cut(s) 358, 557, 3329
CsiI ACCWGGT 1 cut(s) 37
CspAI ACCGGT 1 cut(s) 3779
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
DinI GGCGCC 1 cut(s) 2900
DraI TTTAAA 2 cut(s) 541, 2160
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 6 cut(s) 1197, 2343, 2621, 2667, 3474, 3703
EarI CTCTTC 6 cut(s) 1197, 2343, 2621, 2667, 3474, 3703
Ecl136II GAGCTC 1 cut(s) 2085
Eco130I CCWWGG 3 cut(s) 2622, 3234, 3346
Eco147I AGGCCT 2 cut(s) 1184, 3597
Eco24I GRGCYC 1 cut(s) 2087
Eco31I GGTCTC 1 cut(s) 3356
Eco47I GGWCC 4 cut(s) 1318, 1987, 2104, 3931
Eco53kI GAGCTC 1 cut(s) 2085
Eco57I CTGAAG 5 cut(s) 165, 2307, 2691, 3068, 3947
Eco81I CCTNAGG 1 cut(s) 1322
Eco88I CYCGRG 1 cut(s) 1310
Eco91I GGTNACC 1 cut(s) 1669
EcoICRI GAGCTC 1 cut(s) 2085
EcoNI CCTNNNNNAGG 3 cut(s) 588, 1493, 2391
EcoO109I RGGNCCY 2 cut(s) 1318, 3931
EcoO65I GGTNACC 1 cut(s) 1669
EcoRI GAATTC 3 cut(s) 1687, 1729, 2262
EcoRII CCWGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
EcoT14I CCWWGG 3 cut(s) 2622, 3234, 3346
EcoT22I ATGCAT 3 cut(s) 1566, 1799, 2128
EcoT38I GRGCYC 1 cut(s) 2087
EgeI GGCGCC 1 cut(s) 2900
EheI GGCGCC 1 cut(s) 2900
ErhI CCWWGG 3 cut(s) 2622, 3234, 3346
Esp3I CGTCTC 2 cut(s) 65, 739
FalI AAGNNNNNCTT 2 cut(s) 2063, 2095
FaqI GGGAC 6 cut(s) 105, 584, 1331, 2117, 3393, 3917
FauNDI CATATG 2 cut(s) 890, 3253
FbaI TGATCA 4 cut(s) 118, 2581, 2865, 2908
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 9 cut(s) 856, 932, 999, 1002, 1118, 1121, 1440, 2229, 2754
FriOI GRGCYC 1 cut(s) 2087
Fsp4HI GCNGC 9 cut(s) 856, 932, 999, 1002, 1118, 1121, 1440, 2229, 2754
FspI TGCGCA 1 cut(s) 1481
GlaI GCGC 3 cut(s) 1171, 1481, 2900
GluI GCNGC 9 cut(s) 856, 932, 999, 1002, 1118, 1121, 1440, 2229, 2754
GsuI CTGGAG 3 cut(s) 923, 3051, 3595
HaeII RGCGCY 1 cut(s) 2902
HaeIII GGCC 4 cut(s) 206, 1184, 1439, 3597
HapII CCGG 3 cut(s) 978, 3373, 3780
HgaI GACGC 3 cut(s) 358, 557, 3329
HhaI GCGC 3 cut(s) 1172, 1482, 2901
Hin1I GRCGYC 2 cut(s) 549, 2899
Hin6I GCGC 3 cut(s) 1170, 1480, 2899
HinP1I GCGC 3 cut(s) 1170, 1480, 2899
HincII GTYRAC 1 cut(s) 1101
HindII GTYRAC 1 cut(s) 1101
HindIII AAGCTT 6 cut(s) 812, 1350, 1721, 2813, 3734, 3818
HpaII CCGG 3 cut(s) 978, 3373, 3780
HphI GGTGA 8 cut(s) 394, 2515, 2963, 3224, 3232, 3335, 3572, 4049
Hpy99I CGWCG 4 cut(s) 352, 2144, 2337, 2542
HpyCH4III ACNGT 9 cut(s) 327, 362, 489, 643, 649, 703, 1021, 1098, 2921
HpyCH4IV ACGT 8 cut(s) 320, 718, 731, 1335, 2064, 2278, 2455, 3109
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 1007, 1117, 1226, 1374, 3850
HpySE526I ACGT 8 cut(s) 320, 718, 731, 1335, 2064, 2278, 2455, 3109
Hsp92I GRCGYC 2 cut(s) 549, 2899
HspAI GCGC 3 cut(s) 1170, 1480, 2899
KasI GGCGCC 1 cut(s) 2898
Kpn2I TCCGGA 2 cut(s) 977, 3372
KpnI GGTACC 1 cut(s) 2176
Ksp22I TGATCA 4 cut(s) 118, 2581, 2865, 2908
LguI GCTCTTC 1 cut(s) 3474
LmnI GCTCC 4 cut(s) 1498, 1627, 2963, 3803
Lsp1109I GCAGC 8 cut(s) 867, 918, 988, 1010, 1107, 1129, 2240, 2765
MabI ACCWGGT 1 cut(s) 37
MaeII ACGT 8 cut(s) 320, 718, 731, 1335, 2064, 2278, 2455, 3109
MbiI CCGCTC 1 cut(s) 802
MfeI CAATTG 3 cut(s) 1112, 1173, 3546
MflI RGATCY 5 cut(s) 56, 2972, 2983, 3712, 3911
MhlI GDGCHC 6 cut(s) 46, 372, 384, 559, 2087, 2382
MlsI TGGCCA 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 206
Mly113I GGCGCC 1 cut(s) 2899
MlyI GAGTC 8 cut(s) 491, 566, 746, 1623, 1986, 2176, 3593, 3952
MmeI TCCRAC 7 cut(s) 1271, 1605, 2154, 3561, 3765, 3792, 3882
Mox20I TGGCCA 1 cut(s) 206
Mph1103I ATGCAT 3 cut(s) 1566, 1799, 2128
MroI TCCGGA 2 cut(s) 977, 3372
MroXI GAANNNNTTC 1 cut(s) 1396
MscI TGGCCA 1 cut(s) 206
MslI CAYNNNNRTG 2 cut(s) 863, 3252
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 3 cut(s) 1001, 1120, 2756
MspCI CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
MspI CCGG 3 cut(s) 978, 3373, 3780
MspR9I CCNGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
MunI CAATTG 3 cut(s) 1112, 1173, 3546
Mva1269I GAATGC 3 cut(s) 239, 1805, 3461
MvaI CCWGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
MwoI GCNNNNNNNGC 6 cut(s) 140, 1007, 1117, 1226, 1374, 3850
NarI GGCGCC 1 cut(s) 2899
NdeI CATATG 2 cut(s) 890, 3253
NheI GCTAGC 1 cut(s) 2404
NlaIV GGNNCC 9 cut(s) 58, 381, 595, 1319, 1500, 2105, 2174, 2900, 3933
NmuCI GTSAC 7 cut(s) 321, 483, 2690, 2786, 2871, 3341, 4037
NsbI TGCGCA 1 cut(s) 1481
NsiI ATGCAT 3 cut(s) 1566, 1799, 2128
NspI RCATGY 1 cut(s) 2838
NspV TTCGAA 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 1310
PagI TCATGA 1 cut(s) 2710
PaqCI CACCTGC 1 cut(s) 3059
PceI AGGCCT 2 cut(s) 1184, 3597
PciI ACATGT 1 cut(s) 2834
PciSI GCTCTTC 1 cut(s) 3474
PctI GAATGC 3 cut(s) 239, 1805, 3461
PdmI GAANNNNTTC 1 cut(s) 1396
PflMI CCANNNNNTGG 1 cut(s) 3235
PfoI TCCNGGA 1 cut(s) 601
PinAI ACCGGT 1 cut(s) 3779
PkrI GCNGC 9 cut(s) 857, 933, 1000, 1003, 1119, 1122, 1441, 2230, 2755
Ple19I CGATCG 1 cut(s) 2785
PleI GAGTC 8 cut(s) 490, 566, 746, 1622, 1985, 2175, 3592, 3952
PluTI GGCGCC 1 cut(s) 2902
PpsI GAGTC 8 cut(s) 490, 566, 746, 1622, 1985, 2175, 3592, 3952
Ppu21I YACGTR 1 cut(s) 2279
PpuMI RGGWCCY 2 cut(s) 1318, 3931
PscI ACATGT 1 cut(s) 2834
PshBI ATTAAT 4 cut(s) 1155, 1767, 2115, 3981
PsiI TTATAA 3 cut(s) 1388, 1968, 3437
Psp124BI GAGCTC 1 cut(s) 2087
Psp1406I AACGTT 3 cut(s) 718, 731, 1335
Psp5II RGGWCCY 2 cut(s) 1318, 3931
Psp6I CCWGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
PspEI GGTNACC 1 cut(s) 1669
PspGI CCWGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
PspN4I GGNNCC 9 cut(s) 58, 381, 595, 1319, 1500, 2105, 2174, 2900, 3933
PspPI GGNCC 4 cut(s) 1318, 1987, 2104, 3931
PspPPI RGGWCCY 2 cut(s) 1318, 3931
PspXI VCTCGAGB 1 cut(s) 1310
PstI CTGCAG 1 cut(s) 1000
PstNI CAGNNNCTG 1 cut(s) 3915
PsuI RGATCY 5 cut(s) 56, 2972, 2983, 3712, 3911
PvuI CGATCG 1 cut(s) 2785
PvuII CAGCTG 2 cut(s) 1001, 1120
RseI CAYNNNNRTG 2 cut(s) 863, 3252
SacI GAGCTC 1 cut(s) 2087
SapI GCTCTTC 1 cut(s) 3474
SatI GCNGC 9 cut(s) 856, 932, 999, 1002, 1118, 1121, 1440, 2229, 2754
Sau96I GGNCC 4 cut(s) 1318, 1987, 2104, 3931
ScaI AGTACT 1 cut(s) 2218
SchI GAGTC 8 cut(s) 491, 566, 746, 1623, 1986, 2176, 3593, 3952
ScrFI CCNGG 8 cut(s) 39, 553, 603, 902, 1186, 2372, 3030, 3805
SduI GDGCHC 6 cut(s) 46, 372, 384, 559, 2087, 2382
SexAI ACCWGGT 1 cut(s) 37
SfcI CTRYAG 3 cut(s) 996, 1020, 1094
SfoI GGCGCC 1 cut(s) 2900
Sfr274I CTCGAG 1 cut(s) 1310
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 4 cut(s) 1318, 1987, 2104, 3931
SlaI CTCGAG 1 cut(s) 1310
SmiMI CAYNNNNRTG 2 cut(s) 863, 3252
SseBI AGGCCT 2 cut(s) 1184, 3597
SsiI CCGC 4 cut(s) 802, 1440, 1578, 2756
SspDI GGCGCC 1 cut(s) 2898
SspI AATATT 1 cut(s) 771
SstI GAGCTC 1 cut(s) 2087
StuI AGGCCT 2 cut(s) 1184, 3597
StyD4I CCNGG 8 cut(s) 37, 551, 601, 900, 1184, 2370, 3028, 3803
StyI CCWWGG 3 cut(s) 2622, 3234, 3346
TaaI ACNGT 9 cut(s) 327, 362, 489, 643, 649, 703, 1021, 1098, 2921
TaiI ACGT 8 cut(s) 323, 721, 734, 1338, 2067, 2281, 2458, 3112
TaqI TCGA 8 cut(s) 347, 475, 1311, 2139, 2332, 2781, 3298, 3325
TatI WGTACW 3 cut(s) 633, 2216, 2243
TauI GCSGC 1 cut(s) 1442
TscAI CASTG 7 cut(s) 330, 706, 1845, 1875, 1907, 1956, 2646
TseFI GTSAC 7 cut(s) 321, 483, 2690, 2786, 2871, 3341, 4037
TseI GCWGC 8 cut(s) 855, 931, 998, 1001, 1117, 1120, 2228, 2753
Tsp45I GTSAC 7 cut(s) 321, 483, 2690, 2786, 2871, 3341, 4037
TspGWI ACGGA 5 cut(s) 83, 89, 429, 1502, 1989
TspRI CASTG 7 cut(s) 330, 706, 1845, 1875, 1907, 1956, 2646
Van91I CCANNNNNTGG 1 cut(s) 3235
Vha464I CTTAAG 6 cut(s) 1724, 1831, 2090, 3310, 3532, 3943
VneI GTGCAC 1 cut(s) 2378
VpaK11BI GGWCC 4 cut(s) 1318, 1987, 2104, 3931
VspI ATTAAT 4 cut(s) 1155, 1767, 2115, 3981
XagI CCTNNNNNAGG 3 cut(s) 588, 1493, 2391
XceI RCATGY 1 cut(s) 2838
XcmI CCANNNNNNNNNTGG 1 cut(s) 3214
XhoI CTCGAG 1 cut(s) 1310
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 1396
ZrmI AGTACT 1 cut(s) 2218
Zsp2I ATGCAT 3 cut(s) 1566, 1799, 2128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.