Rh2BG458300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000657
Physical Location & Seq
Forward (+)
64859410 .. 64862979
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG458300.1

Sequence Viewer

Length: 3435 bp
ATGGCCGGAGTATTCGACCTCTGTTGCATTTTCCTTGGGTTTTACGTCCTTTGTGTCGTCGCAAGCGAACTTGCTTACAGATACTCGAAGGGGAAGTACAAGTGGATTAAAAGAGAATTGAAGTTTTTGAGTGCTCTTCTTAAAGATTTTGACGAGGTTACCATGTCAGGGAATCAAATAACACAGGAAATTGGAGTGACTGTGCAAGTAATGAAGGAGAGGGAGGAAAACTGGAAGAAGAGAGCAAGAACTGTTGCTGATGAAGCAACCTTGGCCCTTCAAAGCTTTGCAAAACTGACGAAGAGAAGAAGTTATCACAAGCGGCTTCTTTACGTCATTCTTGATCCGGTAGCGGTGGTTGATGCTGTCCTTGCAATGAACATGTTCGAGCAGAGGATAAATAATATTGTTGGGATGAATCGGAAAGGAGTGAAGCAGACCACAGGTGCCGGTGACATTAATGTATCACTGGAGCAGTCAAGGTCCAAGATCAGAAGCCTTATGAATAGATTCAGTGTTGATGAGATTGAGTCGGTCATGTCCAGACCACCAAGAGCTGATGAATTGACTTTATGTCCAAAGTACGTGATGACTGGAGACCTAGATTTGTTGTGTGGCATTCATCATGAGCAAATAAAGTCCGCCGCGATGCATTTACAGCTACTGCATGCTTTCATGGAAGATTTACAGCGGCTCAAAGTGGTGGAAAGTGATATGGAAAGGTGCTGGATGACAGAAGCAATTGAAACCATTGAGGAAGCAAAGGCAGTCATCAACAGAAACCAGAGAATGTGGCTTTCAGAATTCAGTGATGGGATGGCCAGGCGGAAGCTGAAGAAGGGTATAATGCACATTGGCGCCCGCATCTCTGACCTTCAGGAAACAAAAGAAAAATATGGTATCAAATTCATCCGGCGACAATCATCAAAATCTGCCGACAGGTTTTCTCAACAGCATGCCTCTCAATACCTTCCAACTACAGATGATACTTGTTTGTCATCTCCGGTCAGGAACATTCGTAATTGGCTCAGTCAAGTGCAAGAAACAAGCACCAACTCAGTACAAGATGAGTTGGAGCAGGTTCATAAACTCTTGCAACATACAAAAGGAACTGAAGATTATGGAGCTGTTAATTTAAGATATACTTGCTGGGAGCTACTGAAAAAAAAAGCATCCGAAGCAGATCAGCAATTGATGTCAAACATTCCCTCAAGAATGATATTAACCCGAATTAAGAATACTCTTAGTCTTCTTCTGAGATGCCTACAAGTATATTCAATTGAGGTAAGGGTGGATTCATGCTCAGTAGTTGGTTTGGAAGAAGACATACATGCACTGGTCTCAACTCTCACTACTGAGAGTGGATCTGCTAGTTCTATCATATCCATTGTGGGGATGAAAGGCATCGGTAAGACCACACTGGCCAAGAAAGTATATGACCATGATGCCATTCGAAGCCATTTTAAAGTCCGTCGTTGGGTATCTATACCTCAACAGTATGATGATGAAAACGCACTTTTCAGTAGTGCGGGAAACCAGGTGCTGGAGACGCAAAATAAGGGGTATGAAAAACAATTTTTGATGAAATCAATGCAGGATTTCTTCAAGGAACTTGGTAAGAAAAACTCCAGGTGCCTCTTAATTTTTGACAATGTCTCATCAAACAAAGAAATGTATGCTCTCAAGACAGCATTTTCCCCAGGGAGAAATGGGAGTATTGTTCTAATCACACGCAACAAGACCGTTGGTTCAAATGCTGATCAAAATAGCATTCCCCACCAAGTTCGGCTACGAACTAAAGAAGAGAGCTGGGAGCTTTTTAGCCAAATGGTGGAGTTCTCTGGTGAAGAAATGACTAGTGCAAAGGAAGTTGTAGGATGGAAACTTGGAGGCCTCCCACTTGACATCATGACTGTTGGCTTTCTACTGTGGGGGAAGAAAGTCACATCCGAGGAAGTTTTCAGGGCGGTTGACCGTATCACTCAAGGACAGAACCAAAGTCCATGGTCAGAGAATCTGGCAATGATTGAAGAAGAGTTACAGTTCCATATGATTCTGAGAAAATGTTTTTCTTATTTCAAACTCTTCCCTAGAGAATCTGAAATCCCCATTAGAAGAATTCTGGCTTCCTTAGTTGCACAAGGGTTTGTACAACTAAGCAGAGATCAGAAAATAAATCTAGAAAGTGTAGCATTGGAGTATTTATCAGAGTTGATTGGCCGGAGTATGATTCAAGTAGTTCAAAGGAAGCCTAATGGAATGTTCAAAACATGTCGCATGCCTCCTGCTGTGCATGATCTCTTGTTTGAAGACAAAGCCAAACAATCATTCTCATACGCAACTACAGGTTTTGAAGGGCAACTTGCCTTTCAGTTTGATGATAATGGTACTGGTGCCAGTTTCCTTCCCATTTATGGTCTCAACACAAGTTTGCCAAATGCTCTGCGGAAAGAAAGAATTCTAGTTTTTGAGAATGGAGAAGGAAACAAACCAAGAATGGAAGGTGAATTTCTTGGCAGACGCATTAGGGCTGGTGCCTTCTCGCAGCTACTGGTTCTAGACTTTGAATGTGTAAGCGTCTCTCAATTGCCAACAATCCTTGGAAAACTAAAGCAGCTGGCATATCTTGGCTTAAGACGGACTGAGCTGGAGACGATTCCAGCATCTATAGGGAACTTGGTGAATCTTCAAACCCTGGATTTGAAGCATACTCATGTTCACACTCTCCCTGGTTCAATTTGGAAACTGAAGAATCTTCGGCATCTATACATGCACCAGAGATGTCGAATCAAATTTATGGCTTTACCAACAGCCATTTCAATGAGGAATCTGCAGACACTATCAGGTATATTCTTGGGTAAGATTAACCTTGTAAAGGACCGGCTTGACAAGTTGATCCACCTTCGAAAACTGGAATTGGCATTCCAGCTGAAACCAACAGAGCAAAAGGTGTTGGAAAAGTGGATTGTGAAGCTAACATGTTTACAGTCTTTAAAGTTGAAATCATTTAGTGCAAATGGTGAACTTCCAAAGCTAGAGCGTATCTCAGACCTTAAGAACCTGCACAGCCTATATTTATTTGGAAAGCTTGAATACTCATTCATCACTCGACTCCCTGAAAGCCTCACTCATCTTACCTTAGCAGCTTCTAGCCTCAGAAAAGATCCAATGCCAGAGTTAGGGAAGCTTCCAAAGCTGAAATCACTTTCACTGAAGTCTGGTTCTTATCAAGGAACAGGCATGGTATGCTCTACTGATTATTTTCCCCTGCTTTTTGTTCTCAAACTTTGGAATCTAGATACTCTGGGGAAATTGGATGTGCATGAAGGAGCAATGCAAAATCTTAGCGAGTTTGAGATTAAATTTTGCAACAACTTTACAAGCACTACTGGATTGGAACATTTAAAGAACCTTCAGAAATATAGAGTGAACTGA

Protein Analysis

1144

Amino Acids

130.47

Weight (kDa)

9.29

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 440 - 611 3.2e-27 NB-ARC domain
WHD_DRP PF23559 695 - 767 7e-12 Disease resistance protein Winged helix domain
LRR_14 PF23598 846 - 1143 3.7e-39 Leucine-rich repeat region
LRR_8 PF13855 872 - 925 1.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34770
malus_domestica MD17G1025700.v1.1
prunus_persica Prupe.3G040600_v2.0.a1 Prupe.3G040800_v2.0.a1 Prupe.3G041000_v2.0.a1 Prupe.3G041100_v2.0.a1 Prupe.3G041500_v2.0.a1 Prupe.4G109400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037700_v2.0.a1
pyrus_communis pycom09g10040 pycom17g16000
rosa_chinensis RchiOBHm_Chr2g0145591 RchiOBHm_Chr2g0145611
rosa_laevigata RLG00000020146 RLG00000020149 RLG00000020151 RLG00000020154
rosa_multiflora Rmu_co7986250.1_g000001 Rmu_co8305779.1_g000001 Rmu_sc0002718.1_g000011 Rmu_sc0002835.1_g000009 Rmu_sc0002835.1_g000016 Rmu_sc0003046.1_g000007 Rmu_sc0003046.1_g000010 Rmu_sc0004445.1_g000006 Rmu_sc0004810.1_g000002 Rmu_sc0006477.1_g000008 Rmu_sc0014329.1_g000001 Rmu_sc0020976.1_g000003 Rmu_sc0021168.1_g000001 Rmu_sc0022199.1_g000001 Rmu_sc0036991.1_g000001 Rmu_sc0041497.1_g000003
rosa_roxburghii Rroxscaffold_152G00434560 Rroxscaffold_152G00434570 Rroxscaffold_152G00434600 Rroxscaffold_152G00434610 Rroxscaffold_2G00100630 Rroxscaffold_2G00100640 Rroxscaffold_2G00100660 Rroxscaffold_2G00100670 Rroxscaffold_2G00100680 Rroxscaffold_2G00100690
rosa_rugosa Rorug02G0392300 Rorug02G0392400 Rorug02G0392400
rosa_samantha Rh2AG445700 Rh2AG445800 Rh2AG446000 Rh2AG446100 Rh2AG446300 Rh2AG446600 Rh2BG457400 Rh2BG457500 Rh2BG457600 Rh2BG457900 Rh2BG458300 Rh2CG432300 Rh2CG432400 Rh2CG432500 Rh2CG432700 Rh2CG432900 Rh2CG433000 Rh2CG433300 Rh2DG467100 Rh2DG467200 Rh2DG467300 Rh2DG467600 Rh2DG467900 Rh3DG194100
rosa_wichuraiana Rw2G036420 Rw2G036450 Rw2G036470 Rw3G014900 Rw3G014920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1069, 3069
AccB1I GGYRCC 5 cut(s) 446, 857, 1632, 2393, 2534
AccB7I CCANNNNNTGG 2 cut(s) 1543, 1831
AccII CGCG 1 cut(s) 647
AclWI GGATC 4 cut(s) 338, 1372, 2890, 3158
AcoI YGGCCR 4 cut(s) 3, 819, 1422, 2218
AcsI RAATTY 7 cut(s) 803, 905, 2118, 2457, 2507, 2792, 3362
AcuI CTGAAG 6 cut(s) 854, 860, 1134, 2768, 3233, 3398
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 5 cut(s) 98, 584, 1062, 2151, 2389
AfiI CCNNNNNNNGG 8 cut(s) 168, 1392, 1477, 1543, 1831, 2414, 2875, 3179
AflII CTTAAG 2 cut(s) 2632, 3053
AflIII ACRYGT 3 cut(s) 381, 2270, 2978
AhdI GACNNNNNGTC 1 cut(s) 573
AhlI ACTAGT 1 cut(s) 1856
AjnI CCWGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
AjuI GAANNNNNNNTTGG 4 cut(s) 2312, 2344, 2800, 2832
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 7 cut(s) 591, 1345, 1541, 1660, 2423, 2584, 2645
AlwI GGATC 4 cut(s) 338, 1372, 2890, 3158
AlwNI CAGNNNCTG 3 cut(s) 664, 1543, 2551
AoxI GGCC 6 cut(s) 3, 273, 819, 1422, 1891, 2218
ApeKI GCWGC 3 cut(s) 2545, 2614, 3143
ApoI RAATTY 7 cut(s) 803, 905, 2118, 2457, 2507, 2792, 3362
AseI ATTAAT 1 cut(s) 459
Asp700I GAANNNNTTC 3 cut(s) 383, 509, 2457
AspLEI GCGC 1 cut(s) 860
AspS9I GGNCC 3 cut(s) 274, 483, 2878
AsuHPI GGTGA 5 cut(s) 464, 1856, 2516, 2692, 3032
AsuII TTCGAA 2 cut(s) 1453, 2905
AvaII GGWCC 2 cut(s) 483, 2878
BalI TGGCCA 2 cut(s) 821, 1424
BanI GGYRCC 5 cut(s) 446, 857, 1632, 2393, 2534
BarI GAAGNNNNNNTAC 6 cut(s) 80, 112, 1311, 1343, 2022, 2054
BbsI GAAGAC 3 cut(s) 1241, 1329, 2316
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 3 cut(s) 2557, 2626, 3155
BccI CCATC 3 cut(s) 806, 811, 1872
BciT130I CCWGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
BclI TGATCA 1 cut(s) 1759
BcoDI GTCTC 7 cut(s) 591, 1345, 1541, 1660, 2423, 2584, 2645
BcuI ACTAGT 1 cut(s) 1856
BfmI CTRYAG 4 cut(s) 978, 2343, 2667, 2831
BfoI RGCGCY 1 cut(s) 861
BfrI CTTAAG 2 cut(s) 2632, 3053
BfuAI ACCTGC 2 cut(s) 1069, 3069
BisI GCNGC 6 cut(s) 323, 645, 692, 2546, 2615, 3144
BlsI GCNGC 6 cut(s) 324, 646, 693, 2547, 2616, 3145
Bme1390I CCNGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
Bme18I GGWCC 2 cut(s) 483, 2878
BmeRI GACNNNNNGTC 1 cut(s) 573
BmgT120I GGNCC 3 cut(s) 274, 483, 2878
BmiI GGNNCC 5 cut(s) 448, 859, 1634, 2395, 2536
BmrFI CCNGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
BmsI GCATC 9 cut(s) 352, 639, 873, 1181, 1250, 1413, 1435, 2672, 2770
BpiI GAAGAC 3 cut(s) 1241, 1329, 2316
BplI GAGNNNNNCTC 2 cut(s) 3029, 3061
BpmI CTGGAG 5 cut(s) 491, 615, 1565, 1612, 2669
Bpu10I CCTNAGC 1 cut(s) 3139
Bpu14I TTCGAA 2 cut(s) 1453, 2905
BpuEI CTTGAG 3 cut(s) 1195, 1667, 1968
BsaAI YACGTR 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 858
BsaI GGTCTC 3 cut(s) 591, 1345, 2423
BsaJI CCNNGG 9 cut(s) 34, 270, 1699, 1700, 1950, 2003, 2599, 2694, 2728
BsaWI WCCGGW 2 cut(s) 346, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 8 cut(s) 168, 1392, 1477, 1543, 1831, 2414, 2875, 3179
Bse118I RCCGGY 2 cut(s) 449, 2880
Bse3DI GCAATG 3 cut(s) 381, 2028, 3339
BseBI CCWGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
BseDI CCNNGG 9 cut(s) 34, 270, 1699, 1700, 1950, 2003, 2599, 2694, 2728
BseGI GGATG 9 cut(s) 420, 735, 822, 909, 1172, 1401, 1883, 1946, 3322
BseLI CCNNNNNNNGG 8 cut(s) 168, 1392, 1477, 1543, 1831, 2414, 2875, 3179
BseMI GCAATG 3 cut(s) 381, 2028, 3339
BseMII CTCAG 9 cut(s) 1042, 1071, 1247, 1317, 1347, 2048, 2634, 3060, 3169
BseXI GCAGC 3 cut(s) 2557, 2626, 3155
BseYI CCCAGC 2 cut(s) 1149, 1809
BsgI GTGCAG 1 cut(s) 3047
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 6 cut(s) 5, 275, 821, 1424, 1893, 2220
BshNI GGYRCC 5 cut(s) 446, 857, 1632, 2393, 2534
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 7 cut(s) 6, 347, 450, 913, 1004, 2221, 2881
BslI CCNNNNNNNGG 8 cut(s) 168, 1392, 1477, 1543, 1831, 2414, 2875, 3179
BsmAI GTCTC 7 cut(s) 591, 1345, 1541, 1660, 2423, 2584, 2645
BsmBI CGTCTC 3 cut(s) 1541, 2584, 2645
BsmI GAATGC 3 cut(s) 618, 1770, 2921
BsnI GGCC 6 cut(s) 5, 275, 821, 1424, 1893, 2220
Bso31I GGTCTC 3 cut(s) 591, 1345, 2423
Bsp119I TTCGAA 2 cut(s) 1453, 2905
Bsp1286I GDGCHC 1 cut(s) 136
Bsp1407I TGTACA 1 cut(s) 2149
Bsp143I GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
Bsp19I CCATGG 1 cut(s) 2003
BspANI GGCC 6 cut(s) 5, 275, 821, 1424, 1893, 2220
BspCNI CTCAG 9 cut(s) 1041, 1070, 1248, 1316, 1348, 2049, 2635, 3059, 3168
BspFNI CGCG 1 cut(s) 647
BspHI TCATGA 2 cut(s) 625, 1908
BspLI GGNNCC 5 cut(s) 448, 859, 1634, 2395, 2536
BspMAI CTGCAG 1 cut(s) 2835
BspMI ACCTGC 2 cut(s) 1069, 3069
BspPI GGATC 4 cut(s) 338, 1372, 2890, 3158
BspQI GCTCTTC 1 cut(s) 141
BspT104I TTCGAA 2 cut(s) 1453, 2905
BspT107I GGYRCC 5 cut(s) 446, 857, 1632, 2393, 2534
BspTI CTTAAG 2 cut(s) 2632, 3053
BspTNI GGTCTC 3 cut(s) 591, 1345, 2423
BsrDI GCAATG 3 cut(s) 381, 2028, 3339
BsrFI RCCGGY 2 cut(s) 449, 2880
BsrGI TGTACA 1 cut(s) 2149
BssAI RCCGGY 2 cut(s) 449, 2880
BssECI CCNNGG 9 cut(s) 34, 270, 1699, 1700, 1950, 2003, 2599, 2694, 2728
BssMI GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
BssNI GRCGYC 1 cut(s) 858
BssT1I CCWWGG 4 cut(s) 34, 270, 2003, 2599
Bst2UI CCWGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
Bst4CI ACNGT 9 cut(s) 202, 253, 1497, 1744, 1915, 1929, 1976, 2043, 2988
Bst6I CTCTTC 6 cut(s) 141, 233, 296, 1797, 2028, 2090
BstACI GRCGYC 1 cut(s) 858
BstAFI CTTAAG 2 cut(s) 2632, 3053
BstAPI GCANNNNNTGC 1 cut(s) 3246
BstAUI TGTACA 1 cut(s) 2149
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 2 cut(s) 1453, 2905
BstC8I GCNNGC 6 cut(s) 64, 669, 862, 957, 2279, 2619
BstDSI CCRYGG 1 cut(s) 2003
BstEII GGTNACC 1 cut(s) 157
BstENI CCTNNNNNAGG 1 cut(s) 2873
BstF5I GGATG 9 cut(s) 420, 735, 822, 909, 1172, 1401, 1883, 1946, 3322
BstFNI CGCG 1 cut(s) 647
BstH2I RGCGCY 1 cut(s) 861
BstHHI GCGC 1 cut(s) 860
BstKTI GATC 9 cut(s) 346, 492, 1186, 1367, 1762, 2167, 2299, 2898, 3166
BstMAI GTCTC 7 cut(s) 591, 1345, 1541, 1660, 2423, 2584, 2645
BstMBI GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
BstMWI GCNNNNNNNGC 8 cut(s) 263, 272, 371, 658, 1178, 1549, 3193, 3246
BstNI CCWGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
BstNSI RCATGY 8 cut(s) 385, 671, 959, 1334, 2274, 2281, 2773, 2982
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
BstSFI CTRYAG 4 cut(s) 978, 2343, 2667, 2831
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 3 cut(s) 2557, 2626, 3155
BstV2I GAAGAC 3 cut(s) 1241, 1329, 2316
BstX2I RGATCY 2 cut(s) 1364, 3163
BstYI RGATCY 2 cut(s) 1364, 3163
BsuRI GGCC 6 cut(s) 5, 275, 821, 1424, 1893, 2220
BtgI CCRYGG 1 cut(s) 2003
BtgZI GCGATG 1 cut(s) 662
BtsCI GGATG 9 cut(s) 420, 735, 822, 909, 1172, 1401, 1883, 1946, 3322
BtsIMutI CAGTG 6 cut(s) 467, 520, 814, 1334, 1418, 3209
BveI ACCTGC 2 cut(s) 1069, 3069
Cac8I GCNNGC 6 cut(s) 64, 669, 862, 957, 2279, 2619
CaiI CAGNNNCTG 3 cut(s) 664, 1543, 2551
CciI TCATGA 2 cut(s) 625, 1908
CfoI GCGC 1 cut(s) 860
Cfr10I RCCGGY 2 cut(s) 449, 2880
Cfr13I GGNCC 3 cut(s) 274, 483, 2878
CseI GACGC 3 cut(s) 1558, 2529, 2566
CsiI ACCWGGT 1 cut(s) 1536
Csp6I GTAC 5 cut(s) 97, 583, 1061, 2150, 2388
CviQI GTAC 5 cut(s) 97, 583, 1061, 2150, 2388
DinI GGCGCC 1 cut(s) 859
DpnI GATC 9 cut(s) 345, 491, 1185, 1366, 1761, 2166, 2298, 2897, 3165
DpnII GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
DraI TTTAAA 3 cut(s) 1465, 2994, 3405
DriI GACNNNNNGTC 1 cut(s) 573
EaeI YGGCCR 4 cut(s) 3, 819, 1422, 2218
Eam1104I CTCTTC 6 cut(s) 141, 233, 296, 1797, 2028, 2090
Eam1105I GACNNNNNGTC 1 cut(s) 573
EarI CTCTTC 6 cut(s) 141, 233, 296, 1797, 2028, 2090
EciI GGCGGA 2 cut(s) 631, 841
Eco130I CCWWGG 4 cut(s) 34, 270, 2003, 2599
Eco147I AGGCCT 1 cut(s) 1893
Eco31I GGTCTC 3 cut(s) 591, 1345, 2423
Eco47I GGWCC 2 cut(s) 483, 2878
Eco57I CTGAAG 6 cut(s) 854, 860, 1134, 2768, 3233, 3398
Eco91I GGTNACC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 2873
EcoO65I GGTNACC 1 cut(s) 157
EcoRI GAATTC 3 cut(s) 803, 2118, 2457
EcoRII CCWGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
EcoT14I CCWWGG 4 cut(s) 34, 270, 2003, 2599
EcoT22I ATGCAT 1 cut(s) 654
EgeI GGCGCC 1 cut(s) 859
EheI GGCGCC 1 cut(s) 859
ErhI CCWWGG 4 cut(s) 34, 270, 2003, 2599
Esp3I CGTCTC 3 cut(s) 1541, 2584, 2645
FalI AAGNNNNNCTT 4 cut(s) 1129, 1161, 2346, 2378
FauI CCCGC 2 cut(s) 869, 1522
FauNDI CATATG 1 cut(s) 2049
FbaI TGATCA 1 cut(s) 1759
Fnu4HI GCNGC 6 cut(s) 323, 645, 692, 2546, 2615, 3144
FokI GGATG 9 cut(s) 427, 742, 829, 896, 1159, 1408, 1890, 1933, 3329
Fsp4HI GCNGC 6 cut(s) 323, 645, 692, 2546, 2615, 3144
GlaI GCGC 1 cut(s) 859
GluI GCNGC 6 cut(s) 323, 645, 692, 2546, 2615, 3144
GsaI CCCAGC 2 cut(s) 1153, 1813
GsuI CTGGAG 5 cut(s) 491, 615, 1565, 1612, 2669
HaeII RGCGCY 1 cut(s) 861
HaeIII GGCC 6 cut(s) 5, 275, 821, 1424, 1893, 2220
HapII CCGG 7 cut(s) 6, 347, 450, 913, 1004, 2221, 2881
HgaI GACGC 3 cut(s) 1558, 2529, 2566
HhaI GCGC 1 cut(s) 860
Hin1I GRCGYC 1 cut(s) 858
Hin6I GCGC 1 cut(s) 858
HinP1I GCGC 1 cut(s) 858
HincII GTYRAC 1 cut(s) 1972
HindII GTYRAC 1 cut(s) 1972
HindIII AAGCTT 3 cut(s) 283, 3086, 3185
HpaII CCGG 7 cut(s) 6, 347, 450, 913, 1004, 2221, 2881
HphI GGTGA 5 cut(s) 464, 1856, 2516, 2692, 3032
Hpy166II GTNNAC 5 cut(s) 1972, 2719, 2984, 3023, 3430
Hpy8I GTNNAC 5 cut(s) 1972, 2719, 2984, 3023, 3430
Hpy99I CGWCG 2 cut(s) 62, 1476
HpyCH4III ACNGT 9 cut(s) 202, 253, 1497, 1744, 1915, 1929, 1976, 2043, 2988
HpyCH4IV ACGT 3 cut(s) 45, 333, 585
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 272, 371, 658, 1178, 1549, 3193, 3246
HpySE526I ACGT 3 cut(s) 45, 333, 585
Hsp92I GRCGYC 1 cut(s) 858
HspAI GCGC 1 cut(s) 858
KasI GGCGCC 1 cut(s) 857
Ksp22I TGATCA 1 cut(s) 1759
Kzo9I GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 6 cut(s) 472, 1075, 1124, 1153, 1813, 3329
Lsp1109I GCAGC 3 cut(s) 2557, 2626, 3155
LweI GCATC 9 cut(s) 352, 639, 873, 1181, 1250, 1413, 1435, 2672, 2770
MabI ACCWGGT 1 cut(s) 1536
MaeII ACGT 3 cut(s) 45, 333, 585
MaeIII GTNAC 5 cut(s) 157, 196, 452, 1942, 2037
MalI GATC 9 cut(s) 345, 491, 1185, 1366, 1761, 2166, 2298, 2897, 3165
MboI GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
MfeI CAATTG 4 cut(s) 741, 1190, 1278, 2585
MflI RGATCY 2 cut(s) 1364, 3163
MhlI GDGCHC 1 cut(s) 136
MlsI TGGCCA 2 cut(s) 821, 1424
MluNI TGGCCA 2 cut(s) 821, 1424
Mly113I GGCGCC 1 cut(s) 858
MlyI GAGTC 2 cut(s) 539, 3105
MmeI TCCRAC 3 cut(s) 998, 1053, 2934
Mox20I TGGCCA 2 cut(s) 821, 1424
Mph1103I ATGCAT 1 cut(s) 654
MroXI GAANNNNTTC 3 cut(s) 383, 509, 2457
MscI TGGCCA 2 cut(s) 821, 1424
MslI CAYNNNNRTG 2 cut(s) 2712, 2819
Msp20I TGGCCA 2 cut(s) 821, 1424
MspA1I CMGCKG 3 cut(s) 691, 2617, 2929
MspCI CTTAAG 2 cut(s) 2632, 3053
MspI CCGG 7 cut(s) 6, 347, 450, 913, 1004, 2221, 2881
MspR9I CCNGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
MunI CAATTG 4 cut(s) 741, 1190, 1278, 2585
Mva1269I GAATGC 3 cut(s) 618, 1770, 2921
MvaI CCWGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 8 cut(s) 263, 272, 371, 658, 1178, 1549, 3193, 3246
NarI GGCGCC 1 cut(s) 858
NcoI CCATGG 1 cut(s) 2003
NdeI CATATG 1 cut(s) 2049
NdeII GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
NlaIV GGNNCC 5 cut(s) 448, 859, 1634, 2395, 2536
NmuCI GTSAC 3 cut(s) 196, 452, 1942
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 8 cut(s) 385, 671, 959, 1334, 2274, 2281, 2773, 2982
NspV TTCGAA 2 cut(s) 1453, 2905
PaeI GCATGC 3 cut(s) 671, 959, 2281
PagI TCATGA 2 cut(s) 625, 1908
PasI CCCWGGG 1 cut(s) 1700
PceI AGGCCT 1 cut(s) 1893
PciI ACATGT 3 cut(s) 381, 2270, 2978
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 3 cut(s) 618, 1770, 2921
PdmI GAANNNNTTC 3 cut(s) 383, 509, 2457
PflFI GACNNNGTC 1 cut(s) 1652
PflMI CCANNNNNTGG 2 cut(s) 1543, 1831
PkrI GCNGC 6 cut(s) 324, 646, 693, 2547, 2616, 3145
PleI GAGTC 2 cut(s) 538, 3105
PluTI GGCGCC 1 cut(s) 861
PpsI GAGTC 2 cut(s) 538, 3105
Ppu21I YACGTR 1 cut(s) 586
PscI ACATGT 3 cut(s) 381, 2270, 2978
PshBI ATTAAT 1 cut(s) 459
Psp6I CCWGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
PspEI GGTNACC 1 cut(s) 157
PspFI CCCAGC 2 cut(s) 1149, 1809
PspGI CCWGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
PspN4I GGNNCC 5 cut(s) 448, 859, 1634, 2395, 2536
PspPI GGNCC 3 cut(s) 274, 483, 2878
PstI CTGCAG 1 cut(s) 2835
PstNI CAGNNNCTG 3 cut(s) 664, 1543, 2551
PsuI RGATCY 2 cut(s) 1364, 3163
PsyI GACNNNGTC 1 cut(s) 1652
PvuII CAGCTG 2 cut(s) 2617, 2929
RsaI GTAC 5 cut(s) 98, 584, 1062, 2151, 2389
RsaNI GTAC 5 cut(s) 97, 583, 1061, 2150, 2388
RseI CAYNNNNRTG 2 cut(s) 2712, 2819
SapI GCTCTTC 1 cut(s) 141
SatI GCNGC 6 cut(s) 323, 645, 692, 2546, 2615, 3144
Sau3AI GATC 9 cut(s) 343, 489, 1183, 1364, 1759, 2164, 2296, 2895, 3163
Sau96I GGNCC 3 cut(s) 274, 483, 2878
SchI GAGTC 2 cut(s) 539, 3105
ScrFI CCNGG 6 cut(s) 823, 1538, 1630, 1701, 2696, 2730
SduI GDGCHC 1 cut(s) 136
SexAI ACCWGGT 1 cut(s) 1536
SfaNI GCATC 9 cut(s) 352, 639, 873, 1181, 1250, 1413, 1435, 2672, 2770
SfcI CTRYAG 4 cut(s) 978, 2343, 2667, 2831
SfoI GGCGCC 1 cut(s) 859
SfuI TTCGAA 2 cut(s) 1453, 2905
SinI GGWCC 2 cut(s) 483, 2878
SmiMI CAYNNNNRTG 2 cut(s) 2712, 2819
SmlI CTYRAG 5 cut(s) 1210, 1682, 1983, 2632, 3053
SmoI CTYRAG 5 cut(s) 1210, 1682, 1983, 2632, 3053
SpeI ACTAGT 1 cut(s) 1856
SphI GCATGC 3 cut(s) 671, 959, 2281
SseBI AGGCCT 1 cut(s) 1893
SspDI GGCGCC 1 cut(s) 857
SspI AATATT 1 cut(s) 406
StuI AGGCCT 1 cut(s) 1893
StyD4I CCNGG 6 cut(s) 821, 1536, 1628, 1699, 2694, 2728
StyI CCWWGG 4 cut(s) 34, 270, 2003, 2599
TaaI ACNGT 9 cut(s) 202, 253, 1497, 1744, 1915, 1929, 1976, 2043, 2988
TaiI ACGT 3 cut(s) 48, 336, 588
TaqI TCGA 7 cut(s) 15, 86, 387, 1453, 2785, 2905, 3109
TaqII GACCGA 1 cut(s) 523
TatI WGTACW 3 cut(s) 96, 1060, 2149
TauI GCSGC 3 cut(s) 325, 647, 694
TscAI CASTG 6 cut(s) 474, 520, 814, 1341, 1425, 3216
TseFI GTSAC 3 cut(s) 196, 452, 1942
TseI GCWGC 3 cut(s) 2545, 2614, 3143
Tsp45I GTSAC 3 cut(s) 196, 452, 1942
TspGWI ACGGA 2 cut(s) 1460, 2653
TspRI CASTG 6 cut(s) 474, 520, 814, 1341, 1425, 3216
Tth111I GACNNNGTC 1 cut(s) 1652
Van91I CCANNNNNTGG 2 cut(s) 1543, 1831
Vha464I CTTAAG 2 cut(s) 2632, 3053
VpaK11BI GGWCC 2 cut(s) 483, 2878
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 2873
XapI RAATTY 7 cut(s) 803, 905, 2118, 2457, 2507, 2792, 3362
XbaI TCTAGA 3 cut(s) 2179, 2557, 3295
XceI RCATGY 8 cut(s) 385, 671, 959, 1334, 2274, 2281, 2773, 2982
XmnI GAANNNNTTC 3 cut(s) 383, 509, 2457
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.