Belongs to the disease resistance NB-LRR family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 938, 1244 |
| Acc16I | TGCGCA | 1 cut(s) | 3414 |
| Acc65I | GGTACC | 1 cut(s) | 1623 |
| AccB1I | GGYRCC | 3 cut(s) | 1623, 3348, 3477 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1414 |
| AccBSI | CCGCTC | 1 cut(s) | 1621 |
| AccI | GTMKAC | 2 cut(s) | 1017, 1122 |
| AccII | CGCG | 2 cut(s) | 411, 1531 |
| AciI | CCGC | 8 cut(s) | 616, 788, 1621, 1704, 2094, 2284, 3203, 3465 |
| AclWI | GGATC | 8 cut(s) | 174, 194, 207, 249, 798, 1976, 3107, 3306 |
| AcsI | RAATTY | 8 cut(s) | 370, 932, 1506, 1713, 1860, 2088, 2528, 2735 |
| AcuI | CTGAAG | 7 cut(s) | 1193, 2187, 2477, 2582, 2786, 2939, 3347 |
| AfaI | GTAC | 6 cut(s) | 225, 453, 1028, 1599, 1625, 2136 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 233, 1414, 2290, 2996, 3489 |
| AflII | CTTAAG | 1 cut(s) | 3146 |
| AflIII | ACRYGT | 3 cut(s) | 1900, 2255, 2512 |
| AhlI | ACTAGT | 1 cut(s) | 3220 |
| AjiI | CACGTC | 1 cut(s) | 2256 |
| AjnI | CCWGG | 4 cut(s) | 120, 203, 1565, 2431 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 823, 855, 2810, 2842 |
| Alw21I | GWGCWC | 4 cut(s) | 15, 534, 1827, 2668 |
| Alw26I | GTCTC | 6 cut(s) | 28, 324, 2095, 3005, 3296, 3363 |
| Alw44I | GTGCAC | 1 cut(s) | 1823 |
| AlwI | GGATC | 8 cut(s) | 174, 194, 207, 249, 798, 1976, 3107, 3306 |
| Ama87I | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| AoxI | GGCC | 1 cut(s) | 818 |
| ApaLI | GTGCAC | 1 cut(s) | 1823 |
| ApeKI | GCWGC | 3 cut(s) | 143, 581, 2286 |
| ApoI | RAATTY | 8 cut(s) | 370, 932, 1506, 1713, 1860, 2088, 2528, 2735 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 723, 946 |
| Asp718I | GGTACC | 1 cut(s) | 1623 |
| AspLEI | GCGC | 3 cut(s) | 411, 1533, 3415 |
| AspS9I | GGNCC | 2 cut(s) | 1588, 3331 |
| AsuHPI | GGTGA | 6 cut(s) | 613, 769, 1073, 1760, 2966, 3454 |
| AsuII | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| AsuNHI | GCTAGC | 2 cut(s) | 1849, 3037 |
| AvaI | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| AvaII | GGWCC | 2 cut(s) | 1588, 3331 |
| BaeGI | GKGCMC | 1 cut(s) | 1827 |
| BamHI | GGATCC | 1 cut(s) | 199 |
| BanI | GGYRCC | 3 cut(s) | 1623, 3348, 3477 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2118, 2150 |
| BbsI | GAAGAC | 3 cut(s) | 93, 1407, 1971 |
| Bbv12I | GWGCWC | 4 cut(s) | 15, 534, 1827, 2668 |
| BbvCI | CCTCAGC | 1 cut(s) | 3488 |
| BbvI | GCAGC | 3 cut(s) | 155, 568, 2298 |
| BccI | CCATC | 7 cut(s) | 806, 1266, 1813, 1901, 1996, 2468, 2816 |
| BceAI | ACGGC | 4 cut(s) | 52, 1278, 1369, 1530 |
| BciT130I | CCWGG | 4 cut(s) | 122, 205, 1567, 2433 |
| BclI | TGATCA | 2 cut(s) | 1513, 2838 |
| BcoDI | GTCTC | 6 cut(s) | 28, 324, 2095, 3005, 3296, 3363 |
| BcuI | ACTAGT | 1 cut(s) | 3220 |
| BfaI | CTAG | 7 cut(s) | 1637, 1850, 1889, 2570, 3038, 3221, 3245 |
| BfmI | CTRYAG | 3 cut(s) | 576, 1938, 2690 |
| BfrI | CTTAAG | 1 cut(s) | 3146 |
| BglII | AGATCT | 1 cut(s) | 1274 |
| BisI | GCNGC | 3 cut(s) | 144, 582, 2287 |
| BlsI | GCNGC | 3 cut(s) | 145, 583, 2288 |
| Bme1390I | CCNGG | 4 cut(s) | 122, 205, 1567, 2433 |
| Bme18I | GGWCC | 2 cut(s) | 1588, 3331 |
| BmeT110I | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| BmgBI | CACGTC | 1 cut(s) | 2256 |
| BmgT120I | GGNCC | 2 cut(s) | 1588, 3331 |
| BmiI | GGNNCC | 7 cut(s) | 119, 201, 1589, 1625, 3333, 3350, 3479 |
| BmrFI | CCNGG | 4 cut(s) | 122, 205, 1567, 2433 |
| BmrI | ACTGGG | 1 cut(s) | 2667 |
| BmsI | GCATC | 7 cut(s) | 115, 130, 706, 1042, 1117, 1947, 3180 |
| BmtI | GCTAGC | 2 cut(s) | 1853, 3041 |
| BmuI | ACTGGG | 1 cut(s) | 2667 |
| BpiI | GAAGAC | 3 cut(s) | 93, 1407, 1971 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1364, 1396, 3122, 3154 |
| BpmI | CTGGAG | 3 cut(s) | 500, 1141, 2759 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3488 |
| Bpu14I | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| BpuEI | CTTGAG | 4 cut(s) | 892, 2596, 2912, 3323 |
| BsaAI | YACGTR | 1 cut(s) | 1901 |
| BsaI | GGTCTC | 1 cut(s) | 3005 |
| BsaJI | CCNNGG | 5 cut(s) | 203, 1565, 1583, 2535, 3420 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1631, 1661 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 233, 1414, 2290, 2996, 3489 |
| Bse1I | ACTGG | 7 cut(s) | 892, 987, 1691, 2673, 2869, 2970, 3479 |
| Bse3DI | GCAATG | 3 cut(s) | 363, 3288, 3408 |
| BseBI | CCWGG | 4 cut(s) | 122, 205, 1567, 2433 |
| BseDI | CCNNGG | 5 cut(s) | 203, 1565, 1583, 2535, 3420 |
| BseGI | GGATG | 7 cut(s) | 130, 145, 424, 697, 1029, 1404, 3442 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 233, 1414, 2290, 2996, 3489 |
| BseMI | GCAATG | 3 cut(s) | 363, 3288, 3408 |
| BseMII | CTCAG | 7 cut(s) | 240, 825, 1395, 2860, 3048, 3307, 3479 |
| BseNI | ACTGG | 7 cut(s) | 892, 987, 1691, 2673, 2869, 2970, 3479 |
| BseRI | GAGGAG | 5 cut(s) | 761, 1631, 1755, 1958, 3033 |
| BseSI | GKGCMC | 1 cut(s) | 1827 |
| BseXI | GCAGC | 3 cut(s) | 155, 568, 2298 |
| BseYI | CCCAGC | 2 cut(s) | 631, 3454 |
| BsgI | GTGCAG | 1 cut(s) | 3301 |
| Bsh1236I | CGCG | 2 cut(s) | 411, 1531 |
| BshFI | GGCC | 1 cut(s) | 820 |
| BshNI | GGYRCC | 3 cut(s) | 1623, 3348, 3477 |
| BsiHKAI | GWGCWC | 4 cut(s) | 15, 534, 1827, 2668 |
| BsiHKCI | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| BslFI | GGGAC | 2 cut(s) | 881, 3317 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 233, 1414, 2290, 2996, 3489 |
| BsmAI | GTCTC | 6 cut(s) | 28, 324, 2095, 3005, 3296, 3363 |
| BsmBI | CGTCTC | 1 cut(s) | 28 |
| BsmFI | GGGAC | 2 cut(s) | 881, 3317 |
| BsmI | GAATGC | 1 cut(s) | 2864 |
| BsnI | GGCC | 1 cut(s) | 820 |
| Bso31I | GGTCTC | 1 cut(s) | 3005 |
| BsoBI | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| Bsp119I | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| Bsp1286I | GDGCHC | 4 cut(s) | 15, 534, 1827, 2668 |
| Bsp1407I | TGTACA | 1 cut(s) | 1026 |
| BspACI | CCGC | 8 cut(s) | 616, 788, 1621, 1704, 2094, 2284, 3203, 3465 |
| BspANI | GGCC | 1 cut(s) | 820 |
| BspCNI | CTCAG | 7 cut(s) | 241, 824, 1394, 2859, 3049, 3306, 3480 |
| BspFNI | CGCG | 2 cut(s) | 411, 1531 |
| BspLI | GGNNCC | 7 cut(s) | 119, 201, 1589, 1625, 3333, 3350, 3479 |
| BspOI | GCTAGC | 2 cut(s) | 1853, 3041 |
| BspPI | GGATC | 8 cut(s) | 174, 194, 207, 249, 798, 1976, 3107, 3306 |
| BspQI | GCTCTTC | 3 cut(s) | 20, 2075, 2328 |
| BspT104I | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| BspT107I | GGYRCC | 3 cut(s) | 1623, 3348, 3477 |
| BspTI | CTTAAG | 1 cut(s) | 3146 |
| BspTNI | GGTCTC | 1 cut(s) | 3005 |
| BsrBI | CCGCTC | 1 cut(s) | 1621 |
| BsrDI | GCAATG | 3 cut(s) | 363, 3288, 3408 |
| BsrGI | TGTACA | 1 cut(s) | 1026 |
| BsrI | ACTGG | 7 cut(s) | 892, 987, 1691, 2673, 2869, 2970, 3479 |
| BssECI | CCNNGG | 5 cut(s) | 203, 1565, 1583, 2535, 3420 |
| BssT1I | CCWWGG | 3 cut(s) | 1583, 2535, 3420 |
| Bst2UI | CCWGG | 4 cut(s) | 122, 205, 1567, 2433 |
| Bst4CI | ACNGT | 3 cut(s) | 1654, 2356, 2810 |
| BstAFI | CTTAAG | 1 cut(s) | 3146 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 140, 3410, 3484 |
| BstAUI | TGTACA | 1 cut(s) | 1026 |
| BstBAI | YACGTR | 1 cut(s) | 1901 |
| BstBI | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| BstC8I | GCNNGC | 7 cut(s) | 509, 822, 1218, 1851, 2121, 2383, 3039 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 231 |
| BstF5I | GGATG | 7 cut(s) | 130, 145, 424, 697, 1029, 1404, 3442 |
| BstFNI | CGCG | 2 cut(s) | 411, 1531 |
| BstHHI | GCGC | 3 cut(s) | 411, 1533, 3415 |
| BstMAI | GTCTC | 6 cut(s) | 28, 324, 2095, 3005, 3296, 3363 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 140, 578, 694, 794, 1476, 3410, 3464, 3484 |
| BstNI | CCWGG | 4 cut(s) | 122, 205, 1567, 2433 |
| BstNSI | RCATGY | 3 cut(s) | 65, 1111, 3269 |
| BstSCI | CCNGG | 4 cut(s) | 120, 203, 1565, 2431 |
| BstSFI | CTRYAG | 3 cut(s) | 576, 1938, 2690 |
| BstSLI | GKGCMC | 1 cut(s) | 1827 |
| BstUI | CGCG | 2 cut(s) | 411, 1531 |
| BstV1I | GCAGC | 3 cut(s) | 155, 568, 2298 |
| BstV2I | GAAGAC | 3 cut(s) | 93, 1407, 1971 |
| BstX2I | RGATCY | 4 cut(s) | 199, 790, 1274, 3311 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 280 |
| BstYI | RGATCY | 4 cut(s) | 199, 790, 1274, 3311 |
| BsuRI | GGCC | 1 cut(s) | 820 |
| BtgZI | GCGATG | 1 cut(s) | 1068 |
| BtrI | CACGTC | 1 cut(s) | 2256 |
| BtsCI | GGATG | 7 cut(s) | 130, 145, 424, 697, 1029, 1404, 3442 |
| BtsIMutI | CAGTG | 5 cut(s) | 285, 885, 994, 1389, 3075 |
| Cac8I | GCNNGC | 7 cut(s) | 509, 822, 1218, 1851, 2121, 2383, 3039 |
| CfoI | GCGC | 3 cut(s) | 411, 1533, 3415 |
| Cfr13I | GGNCC | 2 cut(s) | 1588, 3331 |
| Csp6I | GTAC | 6 cut(s) | 224, 452, 1027, 1598, 1624, 2135 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1365, 1400 |
| CviQI | GTAC | 6 cut(s) | 224, 452, 1027, 1598, 1624, 2135 |
| DraI | TTTAAA | 3 cut(s) | 906, 1267, 2332 |
| Eco130I | CCWWGG | 3 cut(s) | 1583, 2535, 3420 |
| Eco147I | AGGCCT | 1 cut(s) | 820 |
| Eco31I | GGTCTC | 1 cut(s) | 3005 |
| Eco32I | GATATC | 1 cut(s) | 1910 |
| Eco47I | GGWCC | 2 cut(s) | 1588, 3331 |
| Eco57I | CTGAAG | 7 cut(s) | 1193, 2187, 2477, 2582, 2786, 2939, 3347 |
| Eco88I | CYCGRG | 4 cut(s) | 860, 2415, 3122, 3334 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 231 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3331 |
| EcoRI | GAATTC | 2 cut(s) | 1713, 2528 |
| EcoRII | CCWGG | 4 cut(s) | 120, 203, 1565, 2431 |
| EcoRV | GATATC | 1 cut(s) | 1910 |
| EcoT14I | CCWWGG | 3 cut(s) | 1583, 2535, 3420 |
| EcoT22I | ATGCAT | 2 cut(s) | 159, 1057 |
| ErhI | CCWWGG | 3 cut(s) | 1583, 2535, 3420 |
| Esp3I | CGTCTC | 1 cut(s) | 28 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 777, 809, 1944, 1976 |
| FaqI | GGGAC | 2 cut(s) | 881, 3317 |
| FauI | CCCGC | 2 cut(s) | 2101, 3196 |
| FauNDI | CATATG | 1 cut(s) | 467 |
| FbaI | TGATCA | 2 cut(s) | 1513, 2838 |
| FblI | GTMKAC | 2 cut(s) | 1017, 1122 |
| Fnu4HI | GCNGC | 3 cut(s) | 144, 582, 2287 |
| FokI | GGATG | 7 cut(s) | 137, 152, 431, 684, 1036, 1391, 3449 |
| Fsp4HI | GCNGC | 3 cut(s) | 144, 582, 2287 |
| FspBI | CTAG | 7 cut(s) | 1637, 1850, 1889, 2570, 3038, 3221, 3245 |
| FspI | TGCGCA | 1 cut(s) | 3414 |
| GlaI | GCGC | 3 cut(s) | 410, 1532, 3414 |
| GluI | GCNGC | 3 cut(s) | 144, 582, 2287 |
| GsaI | CCCAGC | 2 cut(s) | 635, 3458 |
| GsuI | CTGGAG | 3 cut(s) | 500, 1141, 2759 |
| HaeIII | GGCC | 1 cut(s) | 820 |
| HhaI | GCGC | 3 cut(s) | 411, 1533, 3415 |
| Hin6I | GCGC | 3 cut(s) | 409, 1531, 3413 |
| HinP1I | GCGC | 3 cut(s) | 409, 1531, 3413 |
| HincII | GTYRAC | 5 cut(s) | 264, 604, 1018, 3378, 3472 |
| HindII | GTYRAC | 5 cut(s) | 264, 604, 1018, 3378, 3472 |
| HindIII | AAGCTT | 2 cut(s) | 1286, 2234 |
| HpaI | GTTAAC | 3 cut(s) | 604, 3378, 3472 |
| HphI | GGTGA | 6 cut(s) | 613, 769, 1073, 1760, 2966, 3454 |
| Hpy166II | GTNNAC | 9 cut(s) | 264, 604, 1018, 1123, 1812, 1825, 2583, 3378, 3472 |
| Hpy8I | GTNNAC | 9 cut(s) | 264, 604, 1018, 1123, 1812, 1825, 2583, 3378, 3472 |
| Hpy99I | CGWCG | 1 cut(s) | 955 |
| HpyCH4III | ACNGT | 3 cut(s) | 1654, 2356, 2810 |
| HpyCH4IV | ACGT | 3 cut(s) | 1900, 2255, 2512 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 140, 578, 694, 794, 1476, 3410, 3464, 3484 |
| HpySE526I | ACGT | 3 cut(s) | 1900, 2255, 2512 |
| HspAI | GCGC | 3 cut(s) | 409, 1531, 3413 |
| KpnI | GGTACC | 1 cut(s) | 1627 |
| Ksp22I | TGATCA | 2 cut(s) | 1513, 2838 |
| KspAI | GTTAAC | 3 cut(s) | 604, 3378, 3472 |
| LguI | GCTCTTC | 3 cut(s) | 20, 2075, 2328 |
| LmnI | GCTCC | 5 cut(s) | 123, 1520, 1618, 2634, 3046 |
| Lsp1109I | GCAGC | 3 cut(s) | 155, 568, 2298 |
| LweI | GCATC | 7 cut(s) | 115, 130, 706, 1042, 1117, 1947, 3180 |
| MaeI | CTAG | 7 cut(s) | 1637, 1850, 1889, 2570, 3038, 3221, 3245 |
| MaeII | ACGT | 3 cut(s) | 1900, 2255, 2512 |
| MaeIII | GTNAC | 7 cut(s) | 1078, 1419, 1693, 1930, 1992, 3150, 3182 |
| MbiI | CCGCTC | 1 cut(s) | 1621 |
| MfeI | CAATTG | 1 cut(s) | 683 |
| MflI | RGATCY | 4 cut(s) | 199, 790, 1274, 3311 |
| MhlI | GDGCHC | 4 cut(s) | 15, 534, 1827, 2668 |
| MlyI | GAGTC | 4 cut(s) | 326, 662, 1226, 1382 |
| MmeI | TCCRAC | 8 cut(s) | 199, 821, 1120, 1885, 2058, 2509, 2808, 3192 |
| Mph1103I | ATGCAT | 2 cut(s) | 159, 1057 |
| MroXI | GAANNNNTTC | 2 cut(s) | 723, 946 |
| MslI | CAYNNNNRTG | 4 cut(s) | 278, 440, 1087, 2655 |
| MspA1I | CMGCKG | 1 cut(s) | 1099 |
| MspCI | CTTAAG | 1 cut(s) | 3146 |
| MspR9I | CCNGG | 4 cut(s) | 122, 205, 1567, 2433 |
| MunI | CAATTG | 1 cut(s) | 683 |
| Mva1269I | GAATGC | 1 cut(s) | 2864 |
| MvaI | CCWGG | 4 cut(s) | 122, 205, 1567, 2433 |
| MvnI | CGCG | 2 cut(s) | 411, 1531 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 140, 578, 694, 794, 1476, 3410, 3464, 3484 |
| NdeI | CATATG | 1 cut(s) | 467 |
| NheI | GCTAGC | 2 cut(s) | 1849, 3037 |
| NlaIV | GGNNCC | 7 cut(s) | 119, 201, 1589, 1625, 3333, 3350, 3479 |
| NmuCI | GTSAC | 3 cut(s) | 1078, 1419, 1930 |
| NsbI | TGCGCA | 1 cut(s) | 3414 |
| NsiI | ATGCAT | 2 cut(s) | 159, 1057 |
| NspI | RCATGY | 3 cut(s) | 65, 1111, 3269 |
| NspV | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| PaeR7I | CTCGAG | 1 cut(s) | 860 |
| PceI | AGGCCT | 1 cut(s) | 820 |
| PciSI | GCTCTTC | 3 cut(s) | 20, 2075, 2328 |
| PctI | GAATGC | 1 cut(s) | 2864 |
| PdmI | GAANNNNTTC | 2 cut(s) | 723, 946 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1414 |
| PfoI | TCCNGGA | 1 cut(s) | 120 |
| PkrI | GCNGC | 3 cut(s) | 145, 583, 2288 |
| PleI | GAGTC | 4 cut(s) | 326, 662, 1226, 1381 |
| PpsI | GAGTC | 4 cut(s) | 326, 662, 1226, 1381 |
| Ppu21I | YACGTR | 1 cut(s) | 1901 |
| PpuMI | RGGWCCY | 1 cut(s) | 3331 |
| PsiI | TTATAA | 2 cut(s) | 938, 1244 |
| Psp5II | RGGWCCY | 1 cut(s) | 3331 |
| Psp6I | CCWGG | 4 cut(s) | 120, 203, 1565, 2431 |
| PspFI | CCCAGC | 2 cut(s) | 631, 3454 |
| PspGI | CCWGG | 4 cut(s) | 120, 203, 1565, 2431 |
| PspN4I | GGNNCC | 7 cut(s) | 119, 201, 1589, 1625, 3333, 3350, 3479 |
| PspPI | GGNCC | 2 cut(s) | 1588, 3331 |
| PspPPI | RGGWCCY | 1 cut(s) | 3331 |
| PspXI | VCTCGAGB | 1 cut(s) | 860 |
| PsuI | RGATCY | 4 cut(s) | 199, 790, 1274, 3311 |
| PvuII | CAGCTG | 1 cut(s) | 1099 |
| RsaI | GTAC | 6 cut(s) | 225, 453, 1028, 1599, 1625, 2136 |
| RsaNI | GTAC | 6 cut(s) | 224, 452, 1027, 1598, 1624, 2135 |
| RseI | CAYNNNNRTG | 4 cut(s) | 278, 440, 1087, 2655 |
| SalI | GTCGAC | 1 cut(s) | 1016 |
| SapI | GCTCTTC | 3 cut(s) | 20, 2075, 2328 |
| SatI | GCNGC | 3 cut(s) | 144, 582, 2287 |
| Sau96I | GGNCC | 2 cut(s) | 1588, 3331 |
| SchI | GAGTC | 4 cut(s) | 326, 662, 1226, 1382 |
| ScrFI | CCNGG | 4 cut(s) | 122, 205, 1567, 2433 |
| SduI | GDGCHC | 4 cut(s) | 15, 534, 1827, 2668 |
| SfaNI | GCATC | 7 cut(s) | 115, 130, 706, 1042, 1117, 1947, 3180 |
| SfcI | CTRYAG | 3 cut(s) | 576, 1938, 2690 |
| Sfr274I | CTCGAG | 1 cut(s) | 860 |
| SfuI | TTCGAA | 4 cut(s) | 103, 286, 732, 930 |
| SinI | GGWCC | 2 cut(s) | 1588, 3331 |
| SlaI | CTCGAG | 1 cut(s) | 860 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 278, 440, 1087, 2655 |
| SmlI | CTYRAG | 6 cut(s) | 860, 871, 2575, 2927, 3146, 3302 |
| SmoI | CTYRAG | 6 cut(s) | 860, 871, 2575, 2927, 3146, 3302 |
| SpeI | ACTAGT | 1 cut(s) | 3220 |
| SseBI | AGGCCT | 1 cut(s) | 820 |
| SsiI | CCGC | 8 cut(s) | 616, 788, 1621, 1704, 2094, 2284, 3203, 3465 |
| SspI | AATATT | 1 cut(s) | 2607 |
| SspMI | CTAG | 7 cut(s) | 1637, 1850, 1889, 2570, 3038, 3221, 3245 |
| StuI | AGGCCT | 1 cut(s) | 820 |
| StyD4I | CCNGG | 4 cut(s) | 120, 203, 1565, 2431 |
| StyI | CCWWGG | 3 cut(s) | 1583, 2535, 3420 |
| TaaI | ACNGT | 3 cut(s) | 1654, 2356, 2810 |
| TaiI | ACGT | 3 cut(s) | 1903, 2258, 2515 |
| TaqII | GACCGA | 1 cut(s) | 535 |
| TatI | WGTACW | 1 cut(s) | 1026 |
| TscAI | CASTG | 5 cut(s) | 285, 892, 994, 1389, 3082 |
| TseFI | GTSAC | 3 cut(s) | 1078, 1419, 1930 |
| TseI | GCWGC | 3 cut(s) | 143, 581, 2286 |
| Tsp45I | GTSAC | 3 cut(s) | 1078, 1419, 1930 |
| TspGWI | ACGGA | 2 cut(s) | 2651, 2975 |
| TspRI | CASTG | 5 cut(s) | 285, 892, 994, 1389, 3082 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1414 |
| Vha464I | CTTAAG | 1 cut(s) | 3146 |
| VneI | GTGCAC | 1 cut(s) | 1823 |
| VpaK11BI | GGWCC | 2 cut(s) | 1588, 3331 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 231 |
| XapI | RAATTY | 8 cut(s) | 370, 932, 1506, 1713, 1860, 2088, 2528, 2735 |
| XbaI | TCTAGA | 2 cut(s) | 1636, 3244 |
| XceI | RCATGY | 3 cut(s) | 65, 1111, 3269 |
| XhoI | CTCGAG | 1 cut(s) | 860 |
| XmiI | GTMKAC | 2 cut(s) | 1017, 1122 |
| XmnI | GAANNNNTTC | 2 cut(s) | 723, 946 |
| XspI | CTAG | 7 cut(s) | 1637, 1850, 1889, 2570, 3038, 3221, 3245 |
| Zsp2I | ATGCAT | 2 cut(s) | 159, 1057 |