Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000657

Physical Location & Seq

Genomic Coordinates Forward (+)

60031771 .. 60035354

Gene Structure 3584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G036450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3525 bp

ATGGCGGACCACACATTTGCCATCTTTTGCATCCTGTTTGTCATTGTCGTTATCCTTTACCTTGTCGCAAGTGAAGGTGCTTTCAGATACTCGGACGGGAAGTACGAGTTGGTCAGCCGAACTAGTCACTCGAACAGCCAGTCAGTTTTCGATTCCGATCAAATCCAAGATGGAACGAGTGCACAAGTACTAAATGAGAGCGAGAAAGGCTGGATAAAGAAAGCAAGAGAATCAGTTGTTGATGCAACTCCGTGCCTCCAAAGCTTTAAAAATTTGAGGAACAGAAGAAATGCCAAATGGTTGCTCTCCATCGTGCTTGATATCGCACCGAGGTTTGATGCTGCCCTTAAGATGAAACGGTTCGACGAAACCATAAATGATAACCTTGGAGTGGATCAGATCGAGAAGAAAGTTTGTGCCAGTGGAATCAATAAATCAGTGGAGCAGTCAAGATCCATGATCAGAAGCCTGCTAGACAGATCTAGTGTACATGAGGTTGAATCTGCCAAGTTCAGACCACCTGTTGGCACAATAAAAGCTGATGAATTAACTTCGTCTCTGAAGACTTTGAGCACTAGACACCCATATTTGATGCAGGGCCAGCTTGAGGTGCACGTAAAATCCACCATGATGCATTTACAGCTACTTCATGCTTTCATGGAAGATGTTCACGGCCTCGAAGTGCTGGAAAGTGAAATGGAAAACACCTGGGTGATGGAAGCGAACGACACCATTAGTCAAGCGAAGGATGTGATTGACACCATTAATGCCACAGAAAACAAGAGAGGATGGATTTCAGAACCGAATAACTGGATTCGTAGGTGGAAGCATAGGGAGGACATAGTGTCTGTTGGCACCCGGATCTCTGGCCTCCTCACAACAAAGGAAAGATTTGGTGTCAATTTCATCAAATTTATTGAGTCATCAAAATCTGCCCACCACTGTCCTAAGCACCAAGCCTTTCAGCATCCAACTGAACCTGATATTGGCTTGTCGGATGCAGTCAACAACATCCGTAACTTGCTCGATCAACTGCCAAAGACGAGCACCAAGTTAGTATCTCAAGTCAGCAAAGTGAATGAGGAGTTGGATAAACTTTTAGAACATACAAAGGAAACTGAAGAGTATGCTGTGAATCTGAGAAATGCTTCCTGGAAGCTGTTGAAGAAAACCGCTTCAGAAGCAAACGAGCAGTACCAGAAAACCGCTTCTAAGTTTCTATCAAAAACAATACTTGTTCACATCAAGAATGCTGTCAATGTTCTTGAGAGATGCCTGAAAGTATATTCCATTGACGCAAGGGAGGAGTCATGCTCAGTAGTTGGTTTGGACGAAGACATACACGAACTGGTCTCGAGACTCACTACTAATAGTGAACCTGCTAGTTCTATCATACCCATTGTGGGGATGAAGGGCATAGGTAAGACCACTCTAGCCAAGCAAGTTTACTACCATGCTAACATTGTGGAGCACTTTCCAGTCCGTCGTTGGGTATCCCTACCTCCATACCAAAACTCTGATGAATTTGCATTTTTAAGAAGTGTTGGGATCCAGGTCCGGGAGACCAAAGAGAAGTATTCGAATGCATACGAATCAAAAGCCTACTGGATCAACAGAGTGCGCAATTTCTTGAGCATGAAAATGTTCCTTTTAGTATTGGACAATGTCGTGTCCATGGAAGTCTGGGATTCTCTCAAACAAGCACTGCCAGAAAGGACTAAAGGGAGCAAGATTGTGCTGATCACACGCAACAAGGCAATTGCTTTAAATGCTGACCGAGATAGCATTCCTCACCAACTGAGGCTACGAACTCAAGAAGAGAGCTGGGAGCTTTTCTCTCAGATAGTGCAAGTGCAGTACAATGATCCCGAGAAAAAGGAAACCAATCGTGTGAAGGAAGTTGTAGGAAGCAGCTTTGGAGGCTTACCACTTGACATTATAGGCTCTGGCAGTTTACTTTGGGGGAAACAAATCACAACCGAAGAAATTTTGAGGGTTCTTGGACGTAGCACTACTCAAGGACAGGACCAAAATCCTTGGTCACTGAACAAGGCAGTAAATGAAGACGACTTGCAAGAGAATCAGAGTTTTAGGCACTGTTTGTCTTACTTTCAGCTCTTCCCGCCGGACTTTGAAATCCCTGTCAGGAGAATAGTGGCTTGGTGGGTTGCAAGAGAGCTGGAAGGAATCAATGAGAATCATATTCCTGAAAACTGGGTTACTCAACGGTTGAGACAAGTTAATGAGATTCGTATTCCTGAAACTCTTGCGAATGAGTATCTACAAGAGTTGATCGGCCGTAACATGATTCAAGTAGTCAAGAAAAAGCCTGATGGGAAAGCCAAGACATGTCGATTGCCTAGTTCTATGGGAGCTCTGTTGTTGCAAGGCCAAGGCGTTAATAGCAGCGAAACTGATCCCTCTGAATCATCTCCTGATCAAGATAATGGTCCTACCGGAACAAATACACAGCAGAATGAAAGCGAACACAAGTCCATTATCTTCTTCGAGACTGTAGAGCGAAATAAACCGAATCAAGAAATATGTGACTTCCTACGCAAAGGCATTGCTGATGGTTTCTTGGGACAGCTACAATTTCTTGACCTTGAACGTGTATTCCAACCACTGTTGCCGAACGCCATCGGAAATTTAAAGCGGCTGGTATATCTGGGCTTAAGGTGGACTTACCTTGAGTCGATTCCAAAATCTATTGGCAAGTTGGTCGAACTCCAGACCCTTGATTTGAAGCATACTTACATTCAGCACAATCTTCCTAGTTCAATCTGGAAACTGAAGAAACTTCGGTATCTCTACTTAAATCACAACTGTCAATTGCCACAATTGACTCGGTTGATATTATTGCCTCGATCAAATACCATTGGCATGAAGGATCTGCAGAAATTGTCTGGTGTGTTTGTGGATAATGTAAATCTGGTTAAGGATGGGCTTGGAAAGTTGATCAACCTTAGAAAATTGGAGTTGGCATTCCAGCTTGACACTTCGGACGAGCAAGATGTAGTGGAAAGTTGGATTGCGAAGCTCAAGTTCTTAGAGTCTTTGAAGTTGAAATCAATTGATAAGAAGAGGAAACTTCGGGATCTGAAATTACAGTTTCTCCCAACCCTTGAGCTACTGTCCCGGTTACATTTGTTTGGAAAGCTTGTAAATCGACAAATCATCAACCAAGTTCCACAAAGCCTAACTCATCTTACCCTCTCTGCCTCTGAAATCGAAGACGATCCAATGCCGGTGCTAGGCACGCTTCAGAATCTCATATCGCTATCTTTCTACTCTGGTTCTTATCTTGGAAGACGCATGGTTTGCCGCATGAATGGGTTTCCGAAGCTTGTAGTTCTGAAGCTTTGTAAGCTTGCTGCCCTGGAAATATTGGATTTGCAAGAAGCAATGCCAAAGCTAAGCAATTTAGTGATTAAATATTCCAAGAAGCCCAAAGTGACTGTTGTTGGATTGACGCGTTTAACCACCGTCCAAGAATTTGAACAATCAGCTGCAGCCGAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009506 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0030054 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 NB-ARC

Protein Analysis

1174

Amino Acids

133.82

Weight (kDa)

8.68

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	445 - 617	2.6e-31	NB-ARC domain
LRR_14	PF23598	865 - 1163	3.7e-35	Leucine-rich repeat region
LRR_8	PF13855	886 - 942	2e-07	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000657)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g34770
malus_domestica	MD17G1025700.v1.1
prunus_persica	Prupe.3G040600_v2.0.a1 Prupe.3G040800_v2.0.a1 Prupe.3G041000_v2.0.a1 Prupe.3G041100_v2.0.a1 Prupe.3G041500_v2.0.a1 Prupe.4G109400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037400_v2.0.a1 Prupe.8G037700_v2.0.a1
pyrus_communis	pycom09g10040 pycom17g16000
rosa_chinensis	RchiOBHm_Chr2g0145591 RchiOBHm_Chr2g0145611
rosa_laevigata	RLG00000020146 RLG00000020149 RLG00000020151 RLG00000020154
rosa_multiflora	Rmu_co7986250.1_g000001 Rmu_co8305779.1_g000001 Rmu_sc0002718.1_g000011 Rmu_sc0002835.1_g000009 Rmu_sc0002835.1_g000016 Rmu_sc0003046.1_g000007 Rmu_sc0003046.1_g000010 Rmu_sc0004445.1_g000006 Rmu_sc0004810.1_g000002 Rmu_sc0006477.1_g000008 Rmu_sc0014329.1_g000001 Rmu_sc0020976.1_g000003 Rmu_sc0021168.1_g000001 Rmu_sc0022199.1_g000001 Rmu_sc0036991.1_g000001 Rmu_sc0041497.1_g000003
rosa_roxburghii	Rroxscaffold_152G00434560 Rroxscaffold_152G00434570 Rroxscaffold_152G00434600 Rroxscaffold_152G00434610 Rroxscaffold_2G00100630 Rroxscaffold_2G00100640 Rroxscaffold_2G00100660 Rroxscaffold_2G00100670 Rroxscaffold_2G00100680 Rroxscaffold_2G00100690
rosa_rugosa	Rorug02G0392300 Rorug02G0392400 Rorug02G0392400
rosa_samantha	Rh2AG445700 Rh2AG445800 Rh2AG446000 Rh2AG446100 Rh2AG446300 Rh2AG446600 Rh2BG457400 Rh2BG457500 Rh2BG457600 Rh2BG457900 Rh2BG458300 Rh2CG432300 Rh2CG432400 Rh2CG432500 Rh2CG432700 Rh2CG432900 Rh2CG433000 Rh2CG433300 Rh2DG467100 Rh2DG467200 Rh2DG467300 Rh2DG467600 Rh2DG467900 Rh3DG194100
rosa_wichuraiana	Rw2G036420 Rw2G036450 Rw2G036470 Rw3G014900 Rw3G014920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1622
Acc36I	ACCTGC	1 cut(s)	1387
AccB1I	GGYRCC	1 cut(s)	854
AccB7I	CCANNNNNTGG	1 cut(s)	524
AccII	CGCG	1 cut(s)	3475
AciI	CCGC	6 cut(s)	5, 1173, 1206, 2123, 2656, 3325
AcoI	YGGCCR	1 cut(s)	2296
AcsI	RAATTY	6 cut(s)	271, 911, 1523, 1986, 2647, 3494
AcuI	CTGAAG	6 cut(s)	581, 1140, 1161, 2813, 3248, 3377
AfaI	GTAC	5 cut(s)	104, 189, 489, 1196, 1859
AfiI	CCNNNNNNNGG	9 cut(s)	391, 524, 607, 986, 1403, 1404, 1489, 1558, 3254
AflII	CTTAAG	2 cut(s)	347, 2674
AflIII	ACRYGT	3 cut(s)	2348, 2611, 3473
AhdI	GACNNNNNGTC	1 cut(s)	844
AhlI	ACTAGT	1 cut(s)	122
AjnI	CCWGG	4 cut(s)	707, 1151, 1551, 3378
AjuI	GAANNNNNNNTTGG	8 cut(s)	92, 124, 969, 1001, 1070, 1102, 2143, 2175
AleI	CACNNNNGTG	1 cut(s)	710
AloI	GAACNNNNNNTCC	2 cut(s)	2601, 2633
Alw21I	GWGCWC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
Alw26I	GTCTC	6 cut(s)	561, 1351, 1357, 1556, 2227, 2504
Alw44I	GTGCAC	2 cut(s)	180, 611
Ama87I	CYCGRG	2 cut(s)	1354, 1868
AoxI	GGCC	5 cut(s)	598, 673, 868, 2296, 2389
ApaLI	GTGCAC	2 cut(s)	180, 611
ApeKI	GCWGC	6 cut(s)	341, 1911, 2406, 3374, 3509, 3512
ApoI	RAATTY	6 cut(s)	271, 911, 1523, 1986, 2647, 3494
ArsI	GACNNNNNNTTYG	7 cut(s)	31, 125, 157, 2725, 2757, 3303, 3335
AseI	ATTAAT	1 cut(s)	765
Asp700I	GAANNNNTTC	4 cut(s)	359, 666, 1147, 1643
AspLEI	GCGC	1 cut(s)	1623
AspS9I	GGNCC	5 cut(s)	7, 598, 1555, 2026, 2450
AsuC2I	CCSGG	3 cut(s)	859, 1559, 3139
AsuHPI	GGTGA	2 cut(s)	724, 1784
AsuII	TTCGAA	1 cut(s)	1580
AvaI	CYCGRG	2 cut(s)	1354, 1868
AvaII	GGWCC	4 cut(s)	7, 1555, 2026, 2450
BaeGI	GKGCMC	2 cut(s)	184, 615
BaeI	ACNNNNGTAYC	2 cut(s)	2789, 2822
BamHI	GGATCC	1 cut(s)	1548
BanI	GGYRCC	1 cut(s)	854
BanII	GRGCYC	1 cut(s)	2377
BarI	GAAGNNNNNNTAC	2 cut(s)	2738, 2770
BbsI	GAAGAC	5 cut(s)	569, 1341, 2070, 3240, 3316
Bbv12I	GWGCWC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
BbvI	GCAGC	5 cut(s)	328, 1923, 2418, 3361, 3496
BccI	CCATC	9 cut(s)	29, 164, 317, 709, 783, 2327, 2567, 2648, 2936
BceAI	ACGGC	2 cut(s)	688, 2283
BcgI	CGANNNNNNTGC	2 cut(s)	2704, 2738
BciT130I	CCWGG	4 cut(s)	709, 1153, 1553, 3380
BciVI	GTATCC	1 cut(s)	1504
BclI	TGATCA	4 cut(s)	459, 1740, 2437, 2958
BcnI	CCSGG	3 cut(s)	859, 1559, 3139
BcoDI	GTCTC	6 cut(s)	561, 1351, 1357, 1556, 2227, 2504
BcuI	ACTAGT	1 cut(s)	122
BfaI	CTAG	9 cut(s)	123, 473, 483, 576, 1383, 1433, 2361, 2775, 3254
BfmI	CTRYAG	3 cut(s)	2514, 2894, 3510
BfrI	CTTAAG	2 cut(s)	347, 2674
BfuAI	ACCTGC	1 cut(s)	1387
BfuI	GTATCC	1 cut(s)	1504
BglII	AGATCT	1 cut(s)	479
BisI	GCNGC	8 cut(s)	342, 1912, 2407, 2657, 3325, 3375, 3510, 3513
BlpI	GCTNAGC	1 cut(s)	3416
BlsI	GCNGC	8 cut(s)	343, 1913, 2408, 2658, 3326, 3376, 3511, 3514
BmcAI	AGTACT	1 cut(s)	189
Bme1390I	CCNGG	7 cut(s)	709, 859, 1153, 1553, 1559, 3139, 3380
Bme18I	GGWCC	4 cut(s)	7, 1555, 2026, 2450
BmeRI	GACNNNNNGTC	1 cut(s)	844
BmeT110I	CYCGRG	2 cut(s)	1354, 1868
BmgT120I	GGNCC	5 cut(s)	7, 598, 1555, 2026, 2450
BmiI	GGNNCC	2 cut(s)	856, 1550
BmrFI	CCNGG	7 cut(s)	709, 859, 1153, 1553, 1559, 3139, 3380
BmrI	ACTGGG	1 cut(s)	2224
BmsI	GCATC	8 cut(s)	39, 232, 328, 582, 621, 976, 988, 1262
BmuI	ACTGGG	1 cut(s)	2224
BpiI	GAAGAC	5 cut(s)	569, 1341, 2070, 3240, 3316
BplI	GAGNNNNNCTC	4 cut(s)	1298, 1330, 1820, 1852
BpmI	CTGGAG	1 cut(s)	2714
Bpu10I	CCTNAGC	1 cut(s)	948
Bpu1102I	GCTNAGC	1 cut(s)	3416
Bpu14I	TTCGAA	1 cut(s)	1580
BpuEI	CTTGAG	9 cut(s)	626, 1047, 1286, 1651, 1797, 2001, 2711, 3026, 3146
BpuMI	CCSGG	3 cut(s)	859, 1559, 3139
BsaAI	YACGTR	1 cut(s)	616
BsaBI	GATNNNNATC	1 cut(s)	156
BsaI	GGTCTC	2 cut(s)	1357, 1556
BsaJI	CCNNGG	8 cut(s)	329, 385, 708, 1674, 2036, 2392, 3378, 3516
BsaWI	WCCGGW	1 cut(s)	2456
BsaXI	ACNNNNNCTCC	2 cut(s)	3099, 3129
Bsc4I	CCNNNNNNNGG	9 cut(s)	391, 524, 607, 986, 1403, 1404, 1489, 1558, 3254
Bse118I	RCCGGY	1 cut(s)	3247
Bse1I	ACTGG	7 cut(s)	139, 420, 815, 1353, 1478, 1610, 2219
Bse3DI	GCAATG	2 cut(s)	2565, 3411
Bse8I	GATNNNNATC	1 cut(s)	156
BseBI	CCWGG	4 cut(s)	709, 1153, 1553, 3380
BseDI	CCNNGG	8 cut(s)	329, 385, 708, 1674, 2036, 2392, 3378, 3516
BseGI	GGATG	8 cut(s)	30, 754, 794, 967, 1003, 1011, 1413, 2947
BseJI	GATNNNNATC	1 cut(s)	156
BseLI	CCNNNNNNNGG	9 cut(s)	391, 524, 607, 986, 1403, 1404, 1489, 1558, 3254
BseMI	GCAATG	2 cut(s)	2565, 3411
BseMII	CTCAG	4 cut(s)	1130, 1329, 1790, 1853
BseNI	ACTGG	7 cut(s)	139, 420, 815, 1353, 1478, 1610, 2219
BseRI	GAGGAG	3 cut(s)	863, 1097, 1319
BseSI	GKGCMC	2 cut(s)	184, 615
BseX3I	CGGCCG	1 cut(s)	2296
BseXI	GCAGC	5 cut(s)	328, 1923, 2418, 3361, 3496
BseYI	CCCAGC	1 cut(s)	1824
BsgI	GTGCAG	1 cut(s)	1874
Bsh1236I	CGCG	1 cut(s)	3475
Bsh1285I	CGRYCG	1 cut(s)	2299
BshFI	GGCC	5 cut(s)	600, 675, 870, 2298, 2391
BshNI	GGYRCC	1 cut(s)	854
BsiEI	CGRYCG	1 cut(s)	2299
BsiHKAI	GWGCWC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
BsiHKCI	CYCGRG	2 cut(s)	1354, 1868
BsiSI	CCGG	6 cut(s)	859, 1558, 2126, 2457, 3139, 3248
BslFI	GGGAC	2 cut(s)	2598, 3121
BslI	CCNNNNNNNGG	9 cut(s)	391, 524, 607, 986, 1403, 1404, 1489, 1558, 3254
BsmAI	GTCTC	6 cut(s)	561, 1351, 1357, 1556, 2227, 2504
BsmBI	CGTCTC	1 cut(s)	561
BsmFI	GGGAC	2 cut(s)	2598, 3121
BsmI	GAATGC	4 cut(s)	1255, 1588, 1785, 2984
BsnI	GGCC	5 cut(s)	600, 675, 870, 2298, 2391
Bso31I	GGTCTC	2 cut(s)	1357, 1556
BsoBI	CYCGRG	2 cut(s)	1354, 1868
Bsp119I	TTCGAA	1 cut(s)	1580
Bsp1286I	GDGCHC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
Bsp1407I	TGTACA	1 cut(s)	487
Bsp1720I	GCTNAGC	1 cut(s)	3416
Bsp19I	CCATGG	1 cut(s)	1674
BspACI	CCGC	6 cut(s)	5, 1173, 1206, 2123, 2656, 3325
BspANI	GGCC	5 cut(s)	600, 675, 870, 2298, 2391
BspCNI	CTCAG	4 cut(s)	1131, 1328, 1791, 1852
BspFNI	CGCG	1 cut(s)	3475
BspLI	GGNNCC	2 cut(s)	856, 1550
BspMAI	CTGCAG	2 cut(s)	2898, 3514
BspMI	ACCTGC	1 cut(s)	1387
BspQI	GCTCTTC	1 cut(s)	2123
BspT104I	TTCGAA	1 cut(s)	1580
BspT107I	GGYRCC	1 cut(s)	854
BspTI	CTTAAG	2 cut(s)	347, 2674
BspTNI	GGTCTC	2 cut(s)	1357, 1556
BsrDI	GCAATG	2 cut(s)	2565, 3411
BsrFI	RCCGGY	1 cut(s)	3247
BsrGI	TGTACA	1 cut(s)	487
BsrI	ACTGG	7 cut(s)	139, 420, 815, 1353, 1478, 1610, 2219
BssAI	RCCGGY	1 cut(s)	3247
BssECI	CCNNGG	8 cut(s)	329, 385, 708, 1674, 2036, 2392, 3378, 3516
BssT1I	CCWWGG	4 cut(s)	385, 1674, 2036, 2392
Bst2UI	CCWGG	4 cut(s)	709, 1153, 1553, 3380
Bst6I	CTCTTC	4 cut(s)	1116, 1812, 2123, 3077
BstAFI	CTTAAG	2 cut(s)	347, 2674
BstAPI	GCANNNNNTGC	1 cut(s)	3321
BstAUI	TGTACA	1 cut(s)	487
BstBAI	YACGTR	1 cut(s)	616
BstBI	TTCGAA	1 cut(s)	1580
BstC8I	GCNNGC	4 cut(s)	470, 602, 3260, 3372
BstDEI	CTNAG	9 cut(s)	948, 1139, 1212, 1315, 1799, 1839, 2966, 3049, 3416
BstDSI	CCRYGG	1 cut(s)	1674
BstF5I	GGATG	8 cut(s)	30, 754, 794, 967, 1003, 1011, 1413, 2947
BstFNI	CGCG	1 cut(s)	3475
BstHHI	GCGC	1 cut(s)	1623
BstMAI	GTCTC	6 cut(s)	561, 1351, 1357, 1556, 2227, 2504
BstMCI	CGRYCG	1 cut(s)	2299
BstNI	CCWGG	4 cut(s)	709, 1153, 1553, 3380
BstNSI	RCATGY	1 cut(s)	2352
BstSCI	CCNGG	7 cut(s)	707, 857, 1151, 1551, 1557, 3137, 3378
BstSFI	CTRYAG	3 cut(s)	2514, 2894, 3510
BstSLI	GKGCMC	2 cut(s)	184, 615
BstUI	CGCG	1 cut(s)	3475
BstV1I	GCAGC	5 cut(s)	328, 1923, 2418, 3361, 3496
BstV2I	GAAGAC	5 cut(s)	569, 1341, 2070, 3240, 3316
BstX2I	RGATCY	6 cut(s)	452, 479, 861, 1548, 2890, 3097
BstYI	RGATCY	6 cut(s)	452, 479, 861, 1548, 2890, 3097
BstZI	CGGCCG	1 cut(s)	2296
BsuI	GTATCC	1 cut(s)	1504
BsuRI	GGCC	5 cut(s)	600, 675, 870, 2298, 2391
BtgI	CCRYGG	1 cut(s)	1674
BtsCI	GGATG	8 cut(s)	30, 754, 794, 967, 1003, 1011, 1413, 2947
BtsI	GCAGTG	1 cut(s)	1703
BtsIMutI	CAGTG	7 cut(s)	427, 444, 940, 1703, 2042, 2095, 2624
BveI	ACCTGC	1 cut(s)	1387
Cac8I	GCNNGC	4 cut(s)	470, 602, 3260, 3372
CfoI	GCGC	1 cut(s)	1623
Cfr10I	RCCGGY	1 cut(s)	3247
Cfr13I	GGNCC	5 cut(s)	7, 598, 1555, 2026, 2450
CseI	GACGC	3 cut(s)	1304, 3321, 3481
Csp6I	GTAC	5 cut(s)	103, 188, 488, 1195, 1858
CviQI	GTAC	5 cut(s)	103, 188, 488, 1195, 1858
DdeI	CTNAG	9 cut(s)	948, 1139, 1212, 1315, 1799, 1839, 2966, 3049, 3416
DraI	TTTAAA	3 cut(s)	268, 1767, 2652
DriI	GACNNNNNGTC	1 cut(s)	844
EaeI	YGGCCR	1 cut(s)	2296
EagI	CGGCCG	1 cut(s)	2296
Eam1104I	CTCTTC	4 cut(s)	1116, 1812, 2123, 3077
Eam1105I	GACNNNNNGTC	1 cut(s)	844
EarI	CTCTTC	4 cut(s)	1116, 1812, 2123, 3077
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	2375
EclXI	CGGCCG	1 cut(s)	2296
Eco130I	CCWWGG	4 cut(s)	385, 1674, 2036, 2392
Eco24I	GRGCYC	1 cut(s)	2377
Eco31I	GGTCTC	2 cut(s)	1357, 1556
Eco32I	GATATC	1 cut(s)	322
Eco47I	GGWCC	4 cut(s)	7, 1555, 2026, 2450
Eco52I	CGGCCG	1 cut(s)	2296
Eco53kI	GAGCTC	1 cut(s)	2375
Eco57I	CTGAAG	6 cut(s)	581, 1140, 1161, 2813, 3248, 3377
Eco88I	CYCGRG	2 cut(s)	1354, 1868
EcoICRI	GAGCTC	1 cut(s)	2375
EcoRII	CCWGG	4 cut(s)	707, 1151, 1551, 3378
EcoRV	GATATC	1 cut(s)	322
EcoT14I	CCWWGG	4 cut(s)	385, 1674, 2036, 2392
EcoT22I	ATGCAT	2 cut(s)	636, 1588
EcoT38I	GRGCYC	1 cut(s)	2377
ErhI	CCWWGG	4 cut(s)	385, 1674, 2036, 2392
Esp3I	CGTCTC	1 cut(s)	561
FalI	AAGNNNNNCTT	2 cut(s)	2668, 2700
FaqI	GGGAC	2 cut(s)	2598, 3121
FauI	CCCGC	1 cut(s)	2130
FbaI	TGATCA	4 cut(s)	459, 1740, 2437, 2958
Fnu4HI	GCNGC	8 cut(s)	342, 1912, 2407, 2657, 3325, 3375, 3510, 3513
FokI	GGATG	8 cut(s)	17, 761, 801, 954, 998, 1010, 1420, 2954
FriOI	GRGCYC	1 cut(s)	2377
Fsp4HI	GCNGC	8 cut(s)	342, 1912, 2407, 2657, 3325, 3375, 3510, 3513
FspBI	CTAG	9 cut(s)	123, 473, 483, 576, 1383, 1433, 2361, 2775, 3254
FspI	TGCGCA	1 cut(s)	1622
GlaI	GCGC	1 cut(s)	1622
GluI	GCNGC	8 cut(s)	342, 1912, 2407, 2657, 3325, 3375, 3510, 3513
GsaI	CCCAGC	1 cut(s)	1828
GsuI	CTGGAG	1 cut(s)	2714
HaeIII	GGCC	5 cut(s)	600, 675, 870, 2298, 2391
HapII	CCGG	6 cut(s)	859, 1558, 2126, 2457, 3139, 3248
HgaI	GACGC	3 cut(s)	1304, 3321, 3481
HhaI	GCGC	1 cut(s)	1623
Hin6I	GCGC	1 cut(s)	1621
HinP1I	GCGC	1 cut(s)	1621
HincII	GTYRAC	1 cut(s)	1006
HindII	GTYRAC	1 cut(s)	1006
HindIII	AAGCTT	5 cut(s)	262, 3158, 3344, 3359, 3368
HpaII	CCGG	6 cut(s)	859, 1558, 2126, 2457, 3139, 3248
HphI	GGTGA	2 cut(s)	724, 1784
Hpy99I	CGWCG	2 cut(s)	368, 1488
HpyAV	CCTTC	6 cut(s)	68, 739, 1405, 1888, 2177, 2881
HpyCH4IV	ACGT	3 cut(s)	615, 2005, 2611
HpyF3I	CTNAG	9 cut(s)	948, 1139, 1212, 1315, 1799, 1839, 2966, 3049, 3416
HpySE526I	ACGT	3 cut(s)	615, 2005, 2611
HspAI	GCGC	1 cut(s)	1621
Ksp22I	TGATCA	4 cut(s)	459, 1740, 2437, 2958
LguI	GCTCTTC	1 cut(s)	2123
LmnI	GCTCC	5 cut(s)	442, 1468, 1725, 1828, 2372
Lsp1109I	GCAGC	5 cut(s)	328, 1923, 2418, 3361, 3496
LweI	GCATC	8 cut(s)	39, 232, 328, 582, 621, 976, 988, 1262
MaeI	CTAG	9 cut(s)	123, 473, 483, 576, 1383, 1433, 2361, 2775, 3254
MaeII	ACGT	3 cut(s)	615, 2005, 2611
MaeIII	GTNAC	8 cut(s)	125, 1016, 2040, 2218, 2300, 2546, 3141, 3454
MfeI	CAATTG	4 cut(s)	1758, 2831, 2840, 3072
MflI	RGATCY	6 cut(s)	452, 479, 861, 1548, 2890, 3097
MhlI	GDGCHC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
MluI	ACGCGT	1 cut(s)	3473
MlyI	GAGTC	6 cut(s)	929, 1316, 1353, 2702, 2839, 3062
MmeI	TCCRAC	6 cut(s)	975, 995, 1068, 2644, 3008, 3445
Mph1103I	ATGCAT	2 cut(s)	636, 1588
MroXI	GAANNNNTTC	4 cut(s)	359, 666, 1147, 1643
MslI	CAYNNNNRTG	4 cut(s)	629, 710, 1640, 2882
MspA1I	CMGCKG	1 cut(s)	3509
MspCI	CTTAAG	2 cut(s)	347, 2674
MspI	CCGG	6 cut(s)	859, 1558, 2126, 2457, 3139, 3248
MspR9I	CCNGG	7 cut(s)	709, 859, 1153, 1553, 1559, 3139, 3380
MunI	CAATTG	4 cut(s)	1758, 2831, 2840, 3072
Mva1269I	GAATGC	4 cut(s)	1255, 1588, 1785, 2984
MvaI	CCWGG	4 cut(s)	709, 1153, 1553, 3380
MvnI	CGCG	1 cut(s)	3475
NciI	CCSGG	3 cut(s)	859, 1559, 3139
NcoI	CCATGG	1 cut(s)	1674
NlaIV	GGNNCC	2 cut(s)	856, 1550
NmuCI	GTSAC	4 cut(s)	125, 2040, 2546, 3454
NsbI	TGCGCA	1 cut(s)	1622
NsiI	ATGCAT	2 cut(s)	636, 1588
NspI	RCATGY	1 cut(s)	2352
NspV	TTCGAA	1 cut(s)	1580
OliI	CACNNNNGTG	1 cut(s)	710
PaeR7I	CTCGAG	1 cut(s)	1354
PciI	ACATGT	1 cut(s)	2348
PciSI	GCTCTTC	1 cut(s)	2123
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	4 cut(s)	1255, 1588, 1785, 2984
PdmI	GAANNNNTTC	4 cut(s)	359, 666, 1147, 1643
PflFI	GACNNNGTC	1 cut(s)	1664
PflMI	CCANNNNNTGG	1 cut(s)	524
PfoI	TCCNGGA	2 cut(s)	1151, 1557
PkrI	GCNGC	8 cut(s)	343, 1913, 2408, 2658, 3326, 3376, 3511, 3514
PleI	GAGTC	6 cut(s)	928, 1315, 1353, 2701, 2839, 3061
PpsI	GAGTC	6 cut(s)	928, 1315, 1353, 2701, 2839, 3061
Ppu21I	YACGTR	1 cut(s)	616
PscI	ACATGT	1 cut(s)	2348
PshBI	ATTAAT	1 cut(s)	765
Psp124BI	GAGCTC	1 cut(s)	2377
Psp6I	CCWGG	4 cut(s)	707, 1151, 1551, 3378
PspFI	CCCAGC	1 cut(s)	1824
PspGI	CCWGG	4 cut(s)	707, 1151, 1551, 3378
PspN4I	GGNNCC	2 cut(s)	856, 1550
PspPI	GGNCC	5 cut(s)	7, 598, 1555, 2026, 2450
PstI	CTGCAG	2 cut(s)	2898, 3514
PsuI	RGATCY	6 cut(s)	452, 479, 861, 1548, 2890, 3097
PsyI	GACNNNGTC	1 cut(s)	1664
PvuII	CAGCTG	1 cut(s)	3509
RsaI	GTAC	5 cut(s)	104, 189, 489, 1196, 1859
RsaNI	GTAC	5 cut(s)	103, 188, 488, 1195, 1858
RseI	CAYNNNNRTG	4 cut(s)	629, 710, 1640, 2882
SacI	GAGCTC	1 cut(s)	2377
SapI	GCTCTTC	1 cut(s)	2123
SatI	GCNGC	8 cut(s)	342, 1912, 2407, 2657, 3325, 3375, 3510, 3513
Sau96I	GGNCC	5 cut(s)	7, 598, 1555, 2026, 2450
ScaI	AGTACT	1 cut(s)	189
SchI	GAGTC	6 cut(s)	929, 1316, 1353, 2702, 2839, 3062
ScrFI	CCNGG	7 cut(s)	709, 859, 1153, 1553, 1559, 3139, 3380
SduI	GDGCHC	6 cut(s)	184, 575, 615, 1049, 1473, 2377
SfaNI	GCATC	8 cut(s)	39, 232, 328, 582, 621, 976, 988, 1262
SfcI	CTRYAG	3 cut(s)	2514, 2894, 3510
Sfr274I	CTCGAG	1 cut(s)	1354
SfuI	TTCGAA	1 cut(s)	1580
SinI	GGWCC	4 cut(s)	7, 1555, 2026, 2450
SlaI	CTCGAG	1 cut(s)	1354
SmiMI	CAYNNNNRTG	4 cut(s)	629, 710, 1640, 2882
SpeI	ACTAGT	1 cut(s)	122
SsiI	CCGC	6 cut(s)	5, 1173, 1206, 2123, 2656, 3325
SspI	AATATT	2 cut(s)	3387, 3437
SspMI	CTAG	9 cut(s)	123, 473, 483, 576, 1383, 1433, 2361, 2775, 3254
SstI	GAGCTC	1 cut(s)	2377
StyD4I	CCNGG	7 cut(s)	707, 857, 1151, 1551, 1557, 3137, 3378
StyI	CCWWGG	4 cut(s)	385, 1674, 2036, 2392
TaiI	ACGT	3 cut(s)	618, 2008, 2614
TaqII	GACCGA	1 cut(s)	1791
TatI	WGTACW	3 cut(s)	187, 487, 1857
TauI	GCSGC	2 cut(s)	2659, 3327
TscAI	CASTG	7 cut(s)	427, 444, 947, 1710, 2049, 2102, 2631
TseFI	GTSAC	4 cut(s)	125, 2040, 2546, 3454
TseI	GCWGC	6 cut(s)	341, 1911, 2406, 3374, 3509, 3512
Tsp45I	GTSAC	4 cut(s)	125, 2040, 2546, 3454
TspGWI	ACGGA	3 cut(s)	240, 1004, 1472
TspRI	CASTG	7 cut(s)	427, 444, 947, 1710, 2049, 2102, 2631
Tth111I	GACNNNGTC	1 cut(s)	1664
Van91I	CCANNNNNTGG	1 cut(s)	524
Vha464I	CTTAAG	2 cut(s)	347, 2674
VneI	GTGCAC	2 cut(s)	180, 611
VpaK11BI	GGWCC	4 cut(s)	7, 1555, 2026, 2450
VspI	ATTAAT	1 cut(s)	765
XapI	RAATTY	6 cut(s)	271, 911, 1523, 1986, 2647, 3494
XceI	RCATGY	1 cut(s)	2352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1485
XhoI	CTCGAG	1 cut(s)	1354
XmnI	GAANNNNTTC	4 cut(s)	359, 666, 1147, 1643
XspI	CTAG	9 cut(s)	123, 473, 483, 576, 1383, 1433, 2361, 2775, 3254
ZrmI	AGTACT	1 cut(s)	189
Zsp2I	ATGCAT	2 cut(s)	636, 1588

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.