Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1210, 3210 |
| AccB1I | GGYRCC | 5 cut(s) | 587, 998, 1773, 2534, 2675 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1684, 1972 |
| AccI | GTMKAC | 1 cut(s) | 96 |
| AccII | CGCG | 1 cut(s) | 788 |
| AclWI | GGATC | 4 cut(s) | 479, 1513, 3031, 3299 |
| AcoI | YGGCCR | 4 cut(s) | 144, 960, 1563, 2359 |
| AcsI | RAATTY | 7 cut(s) | 944, 1046, 2259, 2598, 2648, 2933, 3503 |
| AcuI | CTGAAG | 6 cut(s) | 1001, 1275, 2007, 2909, 3374, 3539 |
| AcyI | GRCGYC | 1 cut(s) | 999 |
| AfaI | GTAC | 5 cut(s) | 239, 725, 1203, 2292, 2530 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 146, 309, 1533, 1618, 1684, 1972, 2555, 3016, 3320 |
| AflII | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| AflIII | ACRYGT | 3 cut(s) | 522, 2411, 3119 |
| AhlI | ACTAGT | 1 cut(s) | 1997 |
| AjnI | CCWGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2453, 2485, 2941, 2973 |
| Alw21I | GWGCWC | 1 cut(s) | 277 |
| Alw26I | GTCTC | 8 cut(s) | 49, 732, 1486, 1682, 1801, 2564, 2725, 2786 |
| AlwI | GGATC | 4 cut(s) | 479, 1513, 3031, 3299 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 805, 1684, 2692 |
| AoxI | GGCC | 6 cut(s) | 144, 414, 960, 1563, 2032, 2359 |
| ApeKI | GCWGC | 4 cut(s) | 111, 2686, 2755, 3284 |
| ApoI | RAATTY | 7 cut(s) | 944, 1046, 2259, 2598, 2648, 2933, 3503 |
| AseI | ATTAAT | 3 cut(s) | 63, 558, 600 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 524, 650, 2598 |
| AspLEI | GCGC | 2 cut(s) | 1001, 2500 |
| AspS9I | GGNCC | 3 cut(s) | 415, 624, 3019 |
| AsuHPI | GGTGA | 5 cut(s) | 39, 605, 2657, 2833, 3173 |
| AsuII | TTCGAA | 2 cut(s) | 1594, 3046 |
| AvaII | GGWCC | 2 cut(s) | 624, 3019 |
| BalI | TGGCCA | 2 cut(s) | 962, 1565 |
| BanI | GGYRCC | 5 cut(s) | 587, 998, 1773, 2534, 2675 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 221, 253, 1452, 1484 |
| BbsI | GAAGAC | 3 cut(s) | 1382, 1470, 2457 |
| Bbv12I | GWGCWC | 1 cut(s) | 277 |
| BbvI | GCAGC | 4 cut(s) | 123, 2698, 2767, 3296 |
| BccI | CCATC | 4 cut(s) | 947, 952, 2013, 3085 |
| BciT130I | CCWGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| BclI | TGATCA | 1 cut(s) | 1900 |
| BcoDI | GTCTC | 8 cut(s) | 49, 732, 1486, 1682, 1801, 2564, 2725, 2786 |
| BcuI | ACTAGT | 1 cut(s) | 1997 |
| BfmI | CTRYAG | 5 cut(s) | 489, 1119, 2484, 2808, 2972 |
| BfoI | RGCGCY | 1 cut(s) | 1002 |
| BfrI | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| BfuAI | ACCTGC | 2 cut(s) | 1210, 3210 |
| BisI | GCNGC | 7 cut(s) | 112, 464, 786, 833, 2687, 2756, 3285 |
| BlsI | GCNGC | 7 cut(s) | 113, 465, 787, 834, 2688, 2757, 3286 |
| Bme1390I | CCNGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| Bme18I | GGWCC | 2 cut(s) | 624, 3019 |
| BmgT120I | GGNCC | 3 cut(s) | 415, 624, 3019 |
| BmiI | GGNNCC | 5 cut(s) | 589, 1000, 1775, 2536, 2677 |
| BmrFI | CCNGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| BpiI | GAAGAC | 3 cut(s) | 1382, 1470, 2457 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3170, 3202 |
| BpmI | CTGGAG | 5 cut(s) | 632, 756, 1706, 1753, 2810 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3280 |
| Bpu14I | TTCGAA | 2 cut(s) | 1594, 3046 |
| BpuEI | CTTGAG | 3 cut(s) | 1336, 1808, 2109 |
| BsaAI | YACGTR | 1 cut(s) | 727 |
| BsaHI | GRCGYC | 1 cut(s) | 999 |
| BsaI | GGTCTC | 3 cut(s) | 732, 1486, 2564 |
| BsaWI | WCCGGW | 1 cut(s) | 1144 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 327, 357 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 146, 309, 1533, 1618, 1684, 1972, 2555, 3016, 3320 |
| Bse118I | RCCGGY | 2 cut(s) | 590, 3021 |
| Bse3DI | GCAATG | 4 cut(s) | 84, 522, 2169, 3480 |
| BseBI | CCWGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 146, 309, 1533, 1618, 1684, 1972, 2555, 3016, 3320 |
| BseMI | GCAATG | 4 cut(s) | 84, 522, 2169, 3480 |
| BseMII | CTCAG | 9 cut(s) | 1183, 1212, 1388, 1458, 1488, 2189, 2775, 3201, 3310 |
| BseXI | GCAGC | 4 cut(s) | 123, 2698, 2767, 3296 |
| BseYI | CCCAGC | 2 cut(s) | 1290, 1950 |
| BsgI | GTGCAG | 1 cut(s) | 3188 |
| Bsh1236I | CGCG | 1 cut(s) | 788 |
| BshFI | GGCC | 6 cut(s) | 146, 416, 962, 1565, 2034, 2361 |
| BshNI | GGYRCC | 5 cut(s) | 587, 998, 1773, 2534, 2675 |
| BsiHKAI | GWGCWC | 1 cut(s) | 277 |
| BsiSI | CCGG | 6 cut(s) | 147, 591, 1054, 1145, 2362, 3022 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 146, 309, 1533, 1618, 1684, 1972, 2555, 3016, 3320 |
| BsmAI | GTCTC | 8 cut(s) | 49, 732, 1486, 1682, 1801, 2564, 2725, 2786 |
| BsmBI | CGTCTC | 3 cut(s) | 1682, 2725, 2786 |
| BsmI | GAATGC | 3 cut(s) | 759, 1911, 3062 |
| BsnI | GGCC | 6 cut(s) | 146, 416, 962, 1565, 2034, 2361 |
| Bso31I | GGTCTC | 3 cut(s) | 732, 1486, 2564 |
| Bsp119I | TTCGAA | 2 cut(s) | 1594, 3046 |
| Bsp1286I | GDGCHC | 1 cut(s) | 277 |
| Bsp1407I | TGTACA | 1 cut(s) | 2290 |
| Bsp143I | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| Bsp19I | CCATGG | 2 cut(s) | 141, 2144 |
| BspANI | GGCC | 6 cut(s) | 146, 416, 962, 1565, 2034, 2361 |
| BspCNI | CTCAG | 9 cut(s) | 1182, 1211, 1389, 1457, 1489, 2190, 2776, 3200, 3309 |
| BspFNI | CGCG | 1 cut(s) | 788 |
| BspHI | TCATGA | 2 cut(s) | 766, 2049 |
| BspLI | GGNNCC | 5 cut(s) | 589, 1000, 1775, 2536, 2677 |
| BspMAI | CTGCAG | 1 cut(s) | 2976 |
| BspMI | ACCTGC | 2 cut(s) | 1210, 3210 |
| BspPI | GGATC | 4 cut(s) | 479, 1513, 3031, 3299 |
| BspQI | GCTCTTC | 1 cut(s) | 282 |
| BspT104I | TTCGAA | 2 cut(s) | 1594, 3046 |
| BspT107I | GGYRCC | 5 cut(s) | 587, 998, 1773, 2534, 2675 |
| BspTI | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| BspTNI | GGTCTC | 3 cut(s) | 732, 1486, 2564 |
| BsrDI | GCAATG | 4 cut(s) | 84, 522, 2169, 3480 |
| BsrFI | RCCGGY | 2 cut(s) | 590, 3021 |
| BsrGI | TGTACA | 1 cut(s) | 2290 |
| BssAI | RCCGGY | 2 cut(s) | 590, 3021 |
| BssMI | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| BssNAI | GTATAC | 1 cut(s) | 97 |
| BssNI | GRCGYC | 1 cut(s) | 999 |
| BssT1I | CCWWGG | 5 cut(s) | 141, 175, 411, 2144, 2740 |
| Bst1107I | GTATAC | 1 cut(s) | 97 |
| Bst2UI | CCWGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| Bst4CI | ACNGT | 9 cut(s) | 94, 343, 394, 1638, 1885, 2056, 2070, 2117, 3129 |
| Bst6I | CTCTTC | 6 cut(s) | 282, 374, 437, 1938, 2169, 2231 |
| BstACI | GRCGYC | 1 cut(s) | 999 |
| BstAFI | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 3387 |
| BstAUI | TGTACA | 1 cut(s) | 2290 |
| BstBAI | YACGTR | 1 cut(s) | 727 |
| BstBI | TTCGAA | 2 cut(s) | 1594, 3046 |
| BstC8I | GCNNGC | 6 cut(s) | 205, 810, 1003, 1098, 2420, 2760 |
| BstDSI | CCRYGG | 2 cut(s) | 141, 2144 |
| BstEII | GGTNACC | 1 cut(s) | 298 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3014 |
| BstFNI | CGCG | 1 cut(s) | 788 |
| BstH2I | RGCGCY | 1 cut(s) | 1002 |
| BstHHI | GCGC | 2 cut(s) | 1001, 2500 |
| BstKTI | GATC | 9 cut(s) | 487, 633, 1327, 1508, 1903, 2308, 2440, 3039, 3307 |
| BstMAI | GTCTC | 8 cut(s) | 49, 732, 1486, 1682, 1801, 2564, 2725, 2786 |
| BstMBI | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| BstNI | CCWGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| BstNSI | RCATGY | 8 cut(s) | 526, 812, 1100, 1475, 2415, 2422, 2914, 3123 |
| BstPI | GGTNACC | 1 cut(s) | 298 |
| BstSCI | CCNGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| BstSFI | CTRYAG | 5 cut(s) | 489, 1119, 2484, 2808, 2972 |
| BstUI | CGCG | 1 cut(s) | 788 |
| BstV1I | GCAGC | 4 cut(s) | 123, 2698, 2767, 3296 |
| BstV2I | GAAGAC | 3 cut(s) | 1382, 1470, 2457 |
| BstX2I | RGATCY | 2 cut(s) | 1505, 3304 |
| BstYI | RGATCY | 2 cut(s) | 1505, 3304 |
| BstZ17I | GTATAC | 1 cut(s) | 97 |
| BsuRI | GGCC | 6 cut(s) | 146, 416, 962, 1565, 2034, 2361 |
| BtgI | CCRYGG | 2 cut(s) | 141, 2144 |
| BtgZI | GCGATG | 1 cut(s) | 803 |
| BtsIMutI | CAGTG | 5 cut(s) | 90, 608, 661, 1475, 1559 |
| BveI | ACCTGC | 2 cut(s) | 1210, 3210 |
| Cac8I | GCNNGC | 6 cut(s) | 205, 810, 1003, 1098, 2420, 2760 |
| CaiI | CAGNNNCTG | 3 cut(s) | 805, 1684, 2692 |
| CciI | TCATGA | 2 cut(s) | 766, 2049 |
| CfoI | GCGC | 2 cut(s) | 1001, 2500 |
| Cfr10I | RCCGGY | 2 cut(s) | 590, 3021 |
| Cfr13I | GGNCC | 3 cut(s) | 415, 624, 3019 |
| CseI | GACGC | 3 cut(s) | 1699, 2670, 2707 |
| CsiI | ACCWGGT | 1 cut(s) | 1677 |
| Csp6I | GTAC | 5 cut(s) | 238, 724, 1202, 2291, 2529 |
| CviQI | GTAC | 5 cut(s) | 238, 724, 1202, 2291, 2529 |
| DinI | GGCGCC | 1 cut(s) | 1000 |
| DpnI | GATC | 9 cut(s) | 486, 632, 1326, 1507, 1902, 2307, 2439, 3038, 3306 |
| DpnII | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| DraI | TTTAAA | 3 cut(s) | 1606, 3135, 3546 |
| EaeI | YGGCCR | 4 cut(s) | 144, 960, 1563, 2359 |
| Eam1104I | CTCTTC | 6 cut(s) | 282, 374, 437, 1938, 2169, 2231 |
| EarI | CTCTTC | 6 cut(s) | 282, 374, 437, 1938, 2169, 2231 |
| EciI | GGCGGA | 2 cut(s) | 772, 982 |
| Eco130I | CCWWGG | 5 cut(s) | 141, 175, 411, 2144, 2740 |
| Eco147I | AGGCCT | 1 cut(s) | 2034 |
| Eco31I | GGTCTC | 3 cut(s) | 732, 1486, 2564 |
| Eco47I | GGWCC | 2 cut(s) | 624, 3019 |
| Eco57I | CTGAAG | 6 cut(s) | 1001, 1275, 2007, 2909, 3374, 3539 |
| Eco91I | GGTNACC | 1 cut(s) | 298 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3014 |
| EcoO65I | GGTNACC | 1 cut(s) | 298 |
| EcoRI | GAATTC | 3 cut(s) | 944, 2259, 2598 |
| EcoRII | CCWGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| EcoT14I | CCWWGG | 5 cut(s) | 141, 175, 411, 2144, 2740 |
| EcoT22I | ATGCAT | 1 cut(s) | 795 |
| EgeI | GGCGCC | 1 cut(s) | 1000 |
| EheI | GGCGCC | 1 cut(s) | 1000 |
| ErhI | CCWWGG | 5 cut(s) | 141, 175, 411, 2144, 2740 |
| Esp3I | CGTCTC | 3 cut(s) | 1682, 2725, 2786 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1270, 1302, 2487, 2519 |
| FauI | CCCGC | 2 cut(s) | 1010, 1663 |
| FauNDI | CATATG | 1 cut(s) | 2190 |
| FbaI | TGATCA | 1 cut(s) | 1900 |
| FblI | GTMKAC | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 7 cut(s) | 112, 464, 786, 833, 2687, 2756, 3285 |
| Fsp4HI | GCNGC | 7 cut(s) | 112, 464, 786, 833, 2687, 2756, 3285 |
| GlaI | GCGC | 2 cut(s) | 1000, 2499 |
| GluI | GCNGC | 7 cut(s) | 112, 464, 786, 833, 2687, 2756, 3285 |
| GsaI | CCCAGC | 2 cut(s) | 1294, 1954 |
| GsuI | CTGGAG | 5 cut(s) | 632, 756, 1706, 1753, 2810 |
| HaeII | RGCGCY | 1 cut(s) | 1002 |
| HaeIII | GGCC | 6 cut(s) | 146, 416, 962, 1565, 2034, 2361 |
| HapII | CCGG | 6 cut(s) | 147, 591, 1054, 1145, 2362, 3022 |
| HgaI | GACGC | 3 cut(s) | 1699, 2670, 2707 |
| HhaI | GCGC | 2 cut(s) | 1001, 2500 |
| Hin1I | GRCGYC | 1 cut(s) | 999 |
| Hin6I | GCGC | 2 cut(s) | 999, 2498 |
| HinP1I | GCGC | 2 cut(s) | 999, 2498 |
| HincII | GTYRAC | 1 cut(s) | 2113 |
| HindII | GTYRAC | 1 cut(s) | 2113 |
| HindIII | AAGCTT | 4 cut(s) | 424, 971, 3227, 3326 |
| HpaII | CCGG | 6 cut(s) | 147, 591, 1054, 1145, 2362, 3022 |
| HphI | GGTGA | 5 cut(s) | 39, 605, 2657, 2833, 3173 |
| Hpy166II | GTNNAC | 6 cut(s) | 97, 2113, 2860, 3125, 3164, 3571 |
| Hpy8I | GTNNAC | 6 cut(s) | 97, 2113, 2860, 3125, 3164, 3571 |
| Hpy99I | CGWCG | 2 cut(s) | 203, 1617 |
| HpyCH4III | ACNGT | 9 cut(s) | 94, 343, 394, 1638, 1885, 2056, 2070, 2117, 3129 |
| HpyCH4IV | ACGT | 3 cut(s) | 186, 474, 726 |
| HpySE526I | ACGT | 3 cut(s) | 186, 474, 726 |
| Hsp92I | GRCGYC | 1 cut(s) | 999 |
| HspAI | GCGC | 2 cut(s) | 999, 2498 |
| KasI | GGCGCC | 1 cut(s) | 998 |
| Ksp22I | TGATCA | 1 cut(s) | 1900 |
| Kzo9I | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| LguI | GCTCTTC | 1 cut(s) | 282 |
| LmnI | GCTCC | 4 cut(s) | 613, 1216, 1294, 1954 |
| Lsp1109I | GCAGC | 4 cut(s) | 123, 2698, 2767, 3296 |
| MabI | ACCWGGT | 1 cut(s) | 1677 |
| MaeII | ACGT | 3 cut(s) | 186, 474, 726 |
| MaeIII | GTNAC | 4 cut(s) | 298, 337, 593, 2083 |
| MalI | GATC | 9 cut(s) | 486, 632, 1326, 1507, 1902, 2307, 2439, 3038, 3306 |
| MboI | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| MfeI | CAATTG | 4 cut(s) | 882, 1331, 1419, 2726 |
| MflI | RGATCY | 2 cut(s) | 1505, 3304 |
| MhlI | GDGCHC | 1 cut(s) | 277 |
| MlsI | TGGCCA | 2 cut(s) | 962, 1565 |
| MluNI | TGGCCA | 2 cut(s) | 962, 1565 |
| Mly113I | GGCGCC | 1 cut(s) | 999 |
| MlyI | GAGTC | 2 cut(s) | 680, 3246 |
| MmeI | TCCRAC | 3 cut(s) | 1139, 1194, 3075 |
| Mox20I | TGGCCA | 2 cut(s) | 962, 1565 |
| Mph1103I | ATGCAT | 1 cut(s) | 795 |
| MroXI | GAANNNNTTC | 3 cut(s) | 524, 650, 2598 |
| MscI | TGGCCA | 2 cut(s) | 962, 1565 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2853, 2960 |
| Msp20I | TGGCCA | 2 cut(s) | 962, 1565 |
| MspA1I | CMGCKG | 3 cut(s) | 832, 2758, 3070 |
| MspCI | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| MspI | CCGG | 6 cut(s) | 147, 591, 1054, 1145, 2362, 3022 |
| MspR9I | CCNGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| MunI | CAATTG | 4 cut(s) | 882, 1331, 1419, 2726 |
| Mva1269I | GAATGC | 3 cut(s) | 759, 1911, 3062 |
| MvaI | CCWGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| MvnI | CGCG | 1 cut(s) | 788 |
| NarI | GGCGCC | 1 cut(s) | 999 |
| NcoI | CCATGG | 2 cut(s) | 141, 2144 |
| NdeI | CATATG | 1 cut(s) | 2190 |
| NdeII | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| NlaIV | GGNNCC | 5 cut(s) | 589, 1000, 1775, 2536, 2677 |
| NmuCI | GTSAC | 3 cut(s) | 337, 593, 2083 |
| NsiI | ATGCAT | 1 cut(s) | 795 |
| NspI | RCATGY | 8 cut(s) | 526, 812, 1100, 1475, 2415, 2422, 2914, 3123 |
| NspV | TTCGAA | 2 cut(s) | 1594, 3046 |
| PaeI | GCATGC | 3 cut(s) | 812, 1100, 2422 |
| PagI | TCATGA | 2 cut(s) | 766, 2049 |
| PasI | CCCWGGG | 1 cut(s) | 1841 |
| PceI | AGGCCT | 1 cut(s) | 2034 |
| PciI | ACATGT | 3 cut(s) | 522, 2411, 3119 |
| PciSI | GCTCTTC | 1 cut(s) | 282 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 204 |
| PctI | GAATGC | 3 cut(s) | 759, 1911, 3062 |
| PdmI | GAANNNNTTC | 3 cut(s) | 524, 650, 2598 |
| PflFI | GACNNNGTC | 1 cut(s) | 1793 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1684, 1972 |
| PkrI | GCNGC | 7 cut(s) | 113, 465, 787, 834, 2688, 2757, 3286 |
| PleI | GAGTC | 2 cut(s) | 679, 3246 |
| PluTI | GGCGCC | 1 cut(s) | 1002 |
| PpsI | GAGTC | 2 cut(s) | 679, 3246 |
| Ppu21I | YACGTR | 1 cut(s) | 727 |
| PscI | ACATGT | 3 cut(s) | 522, 2411, 3119 |
| PshBI | ATTAAT | 3 cut(s) | 63, 558, 600 |
| Psp6I | CCWGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| PspEI | GGTNACC | 1 cut(s) | 298 |
| PspFI | CCCAGC | 2 cut(s) | 1290, 1950 |
| PspGI | CCWGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| PspN4I | GGNNCC | 5 cut(s) | 589, 1000, 1775, 2536, 2677 |
| PspPI | GGNCC | 3 cut(s) | 415, 624, 3019 |
| PstI | CTGCAG | 1 cut(s) | 2976 |
| PstNI | CAGNNNCTG | 3 cut(s) | 805, 1684, 2692 |
| PsuI | RGATCY | 2 cut(s) | 1505, 3304 |
| PsyI | GACNNNGTC | 1 cut(s) | 1793 |
| PvuII | CAGCTG | 2 cut(s) | 2758, 3070 |
| RsaI | GTAC | 5 cut(s) | 239, 725, 1203, 2292, 2530 |
| RsaNI | GTAC | 5 cut(s) | 238, 724, 1202, 2291, 2529 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2853, 2960 |
| SapI | GCTCTTC | 1 cut(s) | 282 |
| SatI | GCNGC | 7 cut(s) | 112, 464, 786, 833, 2687, 2756, 3285 |
| Sau3AI | GATC | 9 cut(s) | 484, 630, 1324, 1505, 1900, 2305, 2437, 3036, 3304 |
| Sau96I | GGNCC | 3 cut(s) | 415, 624, 3019 |
| SchI | GAGTC | 2 cut(s) | 680, 3246 |
| ScrFI | CCNGG | 6 cut(s) | 964, 1679, 1771, 1842, 2837, 2871 |
| SduI | GDGCHC | 1 cut(s) | 277 |
| SexAI | ACCWGGT | 1 cut(s) | 1677 |
| SfcI | CTRYAG | 5 cut(s) | 489, 1119, 2484, 2808, 2972 |
| SfoI | GGCGCC | 1 cut(s) | 1000 |
| SfuI | TTCGAA | 2 cut(s) | 1594, 3046 |
| SinI | GGWCC | 2 cut(s) | 624, 3019 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2853, 2960 |
| SmlI | CTYRAG | 6 cut(s) | 974, 1351, 1823, 2124, 2773, 3194 |
| SmoI | CTYRAG | 6 cut(s) | 974, 1351, 1823, 2124, 2773, 3194 |
| SpeI | ACTAGT | 1 cut(s) | 1997 |
| SphI | GCATGC | 3 cut(s) | 812, 1100, 2422 |
| SseBI | AGGCCT | 1 cut(s) | 2034 |
| SspDI | GGCGCC | 1 cut(s) | 998 |
| StuI | AGGCCT | 1 cut(s) | 2034 |
| StyD4I | CCNGG | 6 cut(s) | 962, 1677, 1769, 1840, 2835, 2869 |
| StyI | CCWWGG | 5 cut(s) | 141, 175, 411, 2144, 2740 |
| TaaI | ACNGT | 9 cut(s) | 94, 343, 394, 1638, 1885, 2056, 2070, 2117, 3129 |
| TaiI | ACGT | 3 cut(s) | 189, 477, 729 |
| TaqI | TCGA | 7 cut(s) | 156, 227, 528, 1594, 2926, 3046, 3250 |
| TaqII | GACCGA | 1 cut(s) | 664 |
| TatI | WGTACW | 3 cut(s) | 237, 1201, 2290 |
| TauI | GCSGC | 3 cut(s) | 466, 788, 835 |
| TscAI | CASTG | 5 cut(s) | 97, 615, 661, 1482, 1566 |
| TseFI | GTSAC | 3 cut(s) | 337, 593, 2083 |
| TseI | GCWGC | 4 cut(s) | 111, 2686, 2755, 3284 |
| Tsp45I | GTSAC | 3 cut(s) | 337, 593, 2083 |
| TspGWI | ACGGA | 2 cut(s) | 1601, 2794 |
| TspRI | CASTG | 5 cut(s) | 97, 615, 661, 1482, 1566 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1793 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1684, 1972 |
| Vha464I | CTTAAG | 3 cut(s) | 974, 2773, 3194 |
| VpaK11BI | GGWCC | 2 cut(s) | 624, 3019 |
| VspI | ATTAAT | 3 cut(s) | 63, 558, 600 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3014 |
| XapI | RAATTY | 7 cut(s) | 944, 1046, 2259, 2598, 2648, 2933, 3503 |
| XbaI | TCTAGA | 3 cut(s) | 2320, 2698, 3436 |
| XceI | RCATGY | 8 cut(s) | 526, 812, 1100, 1475, 2415, 2422, 2914, 3123 |
| XmiI | GTMKAC | 1 cut(s) | 96 |
| XmnI | GAANNNNTTC | 3 cut(s) | 524, 650, 2598 |
| Zsp2I | ATGCAT | 1 cut(s) | 795 |