pycom09g07530

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000297
Physical Location & Seq
Reverse (-)
5714264 .. 5715759
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07530.7

Sequence Viewer

Length: 696 bp
ATGGATGAGGATTATTCAAGTGATGAAGGGCAAGCAATAGCGCCATCTTTGGTGGATCTGTGCATTAACACAGCAATAGATAATTTAAGGCGCCTTGGGGATGTTGGTGAAACCGATTCTCATCTTCTCAAGAGAATTCTACCACATTGTACGGTGGACCAGTTGATGCATATAGAGAAGAGTACCAAAGGTAGAGATTTGACTCCAATTACTGATAAGTTGTGGAAGAAATTCTATGAAAAGGAATTTGGTACTAAGAGGGTGAATGAGGTGATTGAAAGGATGAAGCAAAAGAAAGTCGCGTTCAGATGGATACAATTGTATGAGGCAAAGTTAAAGGAAGTTGATATGGCTGAAAATTTAATGGCGGACAAGTTGAAGAGTCGATATCAAGAGGCAAATGCTCGAAAACAAAGTCGGCAAGTTCAAATTTGCACGAAGGTTCCACCATCAAGTAATAAAAGAAGCTGGGGCAATGGGCCTGGCTACAATGTTACTGGAAAGAGCAACTTGATGAAGAAAGCGAAACTAGATTTTCTGAAAAGTAATGAGGTGAAAAATCTTGCAGCAATGAAGAGAAATGCACTCCAGAAGAGTTACAGTGCTCCTCCCGTGAAGAGGCCAGATGCGTTTTCTGGGAAAGCTTCAGCTTCAAGTTCTAAACCAATCAAGCCCCCAAAGCGCCTCCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

232

Amino Acids

26.24

Weight (kDa)

9.8

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42780 AT2G42780
fragaria_vesca FvH4_6g37640 FvH4_6g37640 FvH4_7g18970 FvH4_7g19430 FvH4_7g20490
malus_domestica MD04G1219900.v1.1 MD09G1156600.v1.1 MD11G1064800.v1.1 MD17G1143100.v1.1
prunus_persica Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1
pyrus_communis pycom09g07530 pycom11g05390 pycom12g21760 pycom17g13620
rosa_chinensis RchiOBHm_Chr1g0363261 RchiOBHm_Chr1g0363291 RchiOBHm_Chr1g0363311 RchiOBHm_Chr1g0363471 RchiOBHm_Chr1g0363891 RchiOBHm_Chr1g0377321 RchiOBHm_Chr2g0097481 RchiOBHm_Chr2g0101011 RchiOBHm_Chr2g0137701 RchiOBHm_Chr2g0150641 RchiOBHm_Chr2g0150701 RchiOBHm_Chr3g0476421 RchiOBHm_Chr5g0057921 RchiOBHm_Chr5g0057931 RchiOBHm_Chr5g0057951 RchiOBHm_Chr5g0057981 RchiOBHm_Chr5g0058001 RchiOBHm_Chr5g0058031 RchiOBHm_Chr5g0058051 RchiOBHm_Chr5g0058081 RchiOBHm_Chr5g0058101
rosa_laevigata RLG00000017036 RLG00000019670 RLG00000020502 RLG00000023768 RLG00000027563 RLG00000027575 RLG00000027577 RLG00000027578 RLG00000034860 RLG00000035193
rosa_multiflora Rmu_co8175074.1_g000001 Rmu_sc0000367.1_g000008 Rmu_sc0001862.1_g000004 Rmu_sc0001944.1_g000004 Rmu_sc0002483.1_g000004 Rmu_sc0004027.1_g000010 Rmu_sc0004458.1_g000001 Rmu_sc0004556.1_g000009 Rmu_sc0007226.1_g000008 Rmu_sc0011095.1_g000003 Rmu_sc0027954.1_g000002 Rmu_sc0028722.1_g000001
rosa_roxburghii Rroxscaffold_1G00022510 Rroxscaffold_1G00028200 Rroxscaffold_2G00097050 Rroxscaffold_2G00106910 Rroxscaffold_2G00106930 Rroxscaffold_2G00141650 Rroxscaffold_4G00293150 Rroxscaffold_4G00293320 Rroxscaffold_6G00405280
rosa_rugosa Rorug01G0305900 Rorug01G0306000 Rorug01G0306200 Rorug01G0307100 Rorug01G0307100 Rorug01G0307200 Rorug01G0310800 Rorug01G0402700 Rorug02G0095200 Rorug02G0344100.1 Rorug02G0421900 Rorug02G0422000 Rorug02G0422100 Rorug02G0422200 Rorug03G0154300 Rorug05G0310800 Rorug05G0310900 Rorug05G0337400
rosa_samantha Rh1AG314800 Rh1AG315000 Rh1AG316200 Rh1AG318700 Rh1AG420300 Rh1DG309100 Rh1DG309300 Rh1DG310600 Rh1DG313200 Rh1DG410200 Rh2AG142500 Rh2AG392100 Rh2AG481600 Rh2BG147600 Rh2BG399400 Rh2BG399600 Rh2BG493800 Rh2DG502800 Rh3BG237100 Rh3CG231600 Rh5BG390600 Rh5DG405200
rosa_wichuraiana Rw1G027900 Rw1G027910 Rw1G028000 Rw1G036850 Rw2G011180 Rw2G032110 Rw2G032120 Rw2G039370 Rw2G039510 Rw3G018680 Rw5G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 90
AccII CGCG 1 cut(s) 302
AciI CCGC 1 cut(s) 368
AclWI GGATC 1 cut(s) 63
AcsI RAATTY 5 cut(s) 135, 230, 245, 358, 429
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 91
AfaI GTAC 3 cut(s) 151, 184, 253
AfiI CCNNNNNNNGG 1 cut(s) 618
AgsI TTSAA 5 cut(s) 18, 278, 379, 428, 654
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 3 cut(s) 468, 644, 650
AluI AGCT 3 cut(s) 468, 644, 650
Alw21I GWGCWC 1 cut(s) 607
AlwI GGATC 1 cut(s) 63
AoxI GGCC 2 cut(s) 479, 620
ApeKI GCWGC 1 cut(s) 566
ApoI RAATTY 5 cut(s) 135, 230, 245, 358, 429
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
Asp700I GAANNNNTTC 1 cut(s) 230
AspLEI GCGC 3 cut(s) 43, 93, 684
AspS9I GGNCC 2 cut(s) 157, 479
AsuHPI GGTGA 4 cut(s) 119, 274, 283, 565
AvaII GGWCC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BanI GGYRCC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 1 cut(s) 578
BccI CCATC 3 cut(s) 52, 303, 457
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 306
BfaI CTAG 1 cut(s) 530
BfoI RGCGCY 3 cut(s) 44, 94, 685
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 2 cut(s) 157, 479
BmiI GGNNCC 2 cut(s) 92, 444
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 156, 616
BpmI CTGGAG 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 94
Bsc4I CCNNNNNNNGG 1 cut(s) 618
Bse1I ACTGG 2 cut(s) 160, 502
Bse3DI GCAATG 2 cut(s) 481, 576
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 3 cut(s) 10, 106, 288
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 618
BseMI GCAATG 2 cut(s) 481, 576
BseNI ACTGG 2 cut(s) 160, 502
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 1 cut(s) 578
BseYI CCCAGC 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 2 cut(s) 481, 622
BshNI GGYRCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 607
BslI CCNNNNNNNGG 1 cut(s) 618
BsnI GGCC 2 cut(s) 481, 622
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 1 cut(s) 55
BspACI CCGC 1 cut(s) 368
BspANI GGCC 2 cut(s) 481, 622
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 2 cut(s) 92, 444
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 90
BsrDI GCAATG 2 cut(s) 481, 576
BsrI ACTGG 2 cut(s) 160, 502
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 1 cut(s) 55
BssNI GRCGYC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 154, 602
Bst6I CTCTTC 5 cut(s) 173, 374, 569, 587, 611
BstACI GRCGYC 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 10, 106, 288
BstFNI CGCG 1 cut(s) 302
BstH2I RGCGCY 3 cut(s) 44, 94, 685
BstHHI GCGC 3 cut(s) 43, 93, 684
BstKTI GATC 1 cut(s) 58
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 679
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 578
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 2 cut(s) 481, 622
BtsCI GGATG 3 cut(s) 10, 106, 288
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 33
CfoI GCGC 3 cut(s) 43, 93, 684
Cfr13I GGNCC 2 cut(s) 157, 479
Csp6I GTAC 3 cut(s) 150, 183, 252
CviJI RGCY 8 cut(s) 353, 468, 481, 486, 622, 644, 650, 673
CviKI_1 RGCY 8 cut(s) 353, 468, 481, 486, 622, 644, 650, 673
CviQI GTAC 3 cut(s) 150, 183, 252
DdeI CTNAG 1 cut(s) 255
DinI GGCGCC 1 cut(s) 92
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
Eam1104I CTCTTC 5 cut(s) 173, 374, 569, 587, 611
EarI CTCTTC 5 cut(s) 173, 374, 569, 587, 611
EciI GGCGGA 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 94
Eco32I GATATC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 481
EcoRV GATATC 1 cut(s) 389
EcoT14I CCWWGG 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 92
EheI GGCGCC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 94
FaiI YATR 5 cut(s) 171, 173, 237, 324, 350
FalI AAGNNNNNCTT 2 cut(s) 494, 526
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 3 cut(s) 17, 113, 295
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 1 cut(s) 530
GlaI GCGC 3 cut(s) 42, 92, 683
GluI GCNGC 1 cut(s) 567
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 1 cut(s) 572
HaeII RGCGCY 3 cut(s) 44, 94, 685
HaeIII GGCC 2 cut(s) 481, 622
HhaI GCGC 3 cut(s) 43, 93, 684
Hin1I GRCGYC 1 cut(s) 91
Hin6I GCGC 3 cut(s) 41, 91, 682
HinP1I GCGC 3 cut(s) 41, 91, 682
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 3 cut(s) 116, 202, 382
HphI GGTGA 4 cut(s) 119, 274, 283, 565
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 3 cut(s) 308, 540, 695
Hpy188III TCNNGA 3 cut(s) 130, 392, 589
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 20, 433
HpyCH4III ACNGT 2 cut(s) 154, 602
HpyCH4V TGCA 5 cut(s) 63, 169, 435, 566, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 679
HpyF3I CTNAG 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 91
HspAI GCGC 3 cut(s) 41, 91, 682
KasI GGCGCC 1 cut(s) 90
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 610
LpnPI CCDG 8 cut(s) 173, 454, 468, 483, 495, 602, 621, 636
Lsp1109I GCAGC 1 cut(s) 578
LweI GCATC 2 cut(s) 156, 616
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 2 cut(s) 493, 596
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 8 cut(s) 116, 190, 238, 391, 529, 586, 604, 628
MfeI CAATTG 1 cut(s) 317
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 8 cut(s) 82, 135, 207, 230, 245, 317, 358, 429
Mly113I GGCGCC 1 cut(s) 91
MlyI GAGTC 2 cut(s) 196, 391
MnlI CCTC 8 cut(s) 252, 262, 319, 388, 544, 612, 618, 695
Mph1103I ATGCAT 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 4 cut(s) 66, 86, 335, 362
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 317
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 1 cut(s) 679
NarI GGCGCC 1 cut(s) 91
NdeII GATC 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 92, 444
NsiI ATGCAT 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 2 cut(s) 196, 390
PluTI GGCGCC 1 cut(s) 94
PpsI GAGTC 2 cut(s) 196, 390
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 92, 444
PspPI GGNCC 2 cut(s) 157, 479
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 3 cut(s) 151, 184, 253
RsaNI GTAC 3 cut(s) 150, 183, 252
SaqAI TTAA 4 cut(s) 66, 86, 335, 362
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 1 cut(s) 55
Sau96I GGNCC 2 cut(s) 157, 479
SchI GAGTC 2 cut(s) 196, 391
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 1 cut(s) 607
SetI ASST 7 cut(s) 193, 273, 444, 470, 555, 646, 652
SfaNI GCATC 2 cut(s) 156, 616
SfoI GGCGCC 1 cut(s) 92
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 8 cut(s) 82, 135, 207, 230, 245, 317, 358, 429
SsiI CCGC 1 cut(s) 368
SspDI GGCGCC 1 cut(s) 90
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 154, 602
TaqI TCGA 2 cut(s) 385, 406
TasI AATT 8 cut(s) 82, 135, 207, 230, 245, 317, 358, 429
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 4 cut(s) 66, 86, 335, 362
Tru9I TTAA 4 cut(s) 66, 86, 335, 362
TscAI CASTG 1 cut(s) 607
TseI GCWGC 1 cut(s) 566
TspDTI ATGAA 5 cut(s) 39, 252, 299, 530, 587
TspRI CASTG 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 5 cut(s) 135, 230, 245, 358, 429
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.