Rorug01G0307100

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000297
Physical Location & Seq
Reverse (-)
42425796 .. 42431935
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0307100.1

Sequence Viewer

Length: 555 bp
ATGAGCAGCGTTGGAGGTGATTCAATGGAGGCCCAATATATAAGGAGGCATCACAGGCACGAACCCAGAGAGAACCAGTGCACTTCTGCTCTGGTTAGGCATGTTAAAGCCCCTGTGCATCTTGTATGGTCATTAGTCAGGAGATTTGATCAACCCCAGAAGTACAAGCCCTTTGTTAGCAGGTGCATCATGAGGGGGGACCTTGGCATTGGGAGTCTTAGAGAAGTGAATGTTAAATCTGGGCTTCCAGCAACAACCAGCACTGAGAGGCTGGAGCTTCTCGATGACGACGAGCACATTCTTGGCATCAGGATCGTTGGTGGTGATCATAGGCTCAGGAATTACTCTTCAATTATTACCGTCCATCCTGAGGTTATAGATGGGAGACCAGGGACACTAGTTATCGAATCCTTTGTGGTGGATGTGCCTGATGGAAATACCAAGGATGAGACATGCTACTTTGTTGAGGCCCTGATCAGGTGCAACCTCAAGTCTTTGGCTGATGTCTCGGAGAGGATGGCTGTCCAGGATCGAACAGAACCTATCAATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

184

Amino Acids

20.82

Weight (kDa)

6.5

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 31 - 171 3.1e-20 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42780 AT2G42780
fragaria_vesca FvH4_6g37640 FvH4_6g37640 FvH4_7g18970 FvH4_7g19430 FvH4_7g20490
malus_domestica MD04G1219900.v1.1 MD09G1156600.v1.1 MD11G1064800.v1.1 MD17G1143100.v1.1
prunus_persica Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1
pyrus_communis pycom09g07530 pycom11g05390 pycom12g21760 pycom17g13620
rosa_chinensis RchiOBHm_Chr1g0363261 RchiOBHm_Chr1g0363291 RchiOBHm_Chr1g0363311 RchiOBHm_Chr1g0363471 RchiOBHm_Chr1g0363891 RchiOBHm_Chr1g0377321 RchiOBHm_Chr2g0097481 RchiOBHm_Chr2g0101011 RchiOBHm_Chr2g0137701 RchiOBHm_Chr2g0150641 RchiOBHm_Chr2g0150701 RchiOBHm_Chr3g0476421 RchiOBHm_Chr5g0057921 RchiOBHm_Chr5g0057931 RchiOBHm_Chr5g0057951 RchiOBHm_Chr5g0057981 RchiOBHm_Chr5g0058001 RchiOBHm_Chr5g0058031 RchiOBHm_Chr5g0058051 RchiOBHm_Chr5g0058081 RchiOBHm_Chr5g0058101
rosa_laevigata RLG00000017036 RLG00000019670 RLG00000020502 RLG00000023768 RLG00000027563 RLG00000027575 RLG00000027577 RLG00000027578 RLG00000034860 RLG00000035193
rosa_multiflora Rmu_co8175074.1_g000001 Rmu_sc0000367.1_g000008 Rmu_sc0001862.1_g000004 Rmu_sc0001944.1_g000004 Rmu_sc0002483.1_g000004 Rmu_sc0004027.1_g000010 Rmu_sc0004458.1_g000001 Rmu_sc0004556.1_g000009 Rmu_sc0007226.1_g000008 Rmu_sc0011095.1_g000003 Rmu_sc0027954.1_g000002 Rmu_sc0028722.1_g000001
rosa_roxburghii Rroxscaffold_1G00022510 Rroxscaffold_1G00028200 Rroxscaffold_2G00097050 Rroxscaffold_2G00106910 Rroxscaffold_2G00106930 Rroxscaffold_2G00141650 Rroxscaffold_4G00293150 Rroxscaffold_4G00293320 Rroxscaffold_6G00405280
rosa_rugosa Rorug01G0305900 Rorug01G0306000 Rorug01G0306200 Rorug01G0307100 Rorug01G0307100 Rorug01G0307200 Rorug01G0310800 Rorug01G0402700 Rorug02G0095200 Rorug02G0344100.1 Rorug02G0421900 Rorug02G0422000 Rorug02G0422100 Rorug02G0422200 Rorug03G0154300 Rorug05G0310800 Rorug05G0310900 Rorug05G0337400
rosa_samantha Rh1AG314800 Rh1AG315000 Rh1AG316200 Rh1AG318700 Rh1AG420300 Rh1DG309100 Rh1DG309300 Rh1DG310600 Rh1DG313200 Rh1DG410200 Rh2AG142500 Rh2AG392100 Rh2AG481600 Rh2BG147600 Rh2BG399400 Rh2BG399600 Rh2BG493800 Rh2DG502800 Rh3BG237100 Rh3CG231600 Rh5BG390600 Rh5DG405200
rosa_wichuraiana Rw1G027900 Rw1G027910 Rw1G028000 Rw1G036850 Rw2G011180 Rw2G032110 Rw2G032120 Rw2G039370 Rw2G039510 Rw3G018680 Rw5G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 171
Acc36I ACCTGC 1 cut(s) 171
AclWI GGATC 2 cut(s) 320, 537
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 370, 477
AgsI TTSAA 2 cut(s) 24, 351
AhlI ACTAGT 1 cut(s) 397
AjnI CCWGG 2 cut(s) 388, 525
AluBI AGCT 1 cut(s) 277
AluI AGCT 1 cut(s) 277
Alw21I GWGCWC 2 cut(s) 83, 297
Alw26I GTCTC 3 cut(s) 379, 443, 511
Alw44I GTGCAC 1 cut(s) 79
AlwI GGATC 2 cut(s) 320, 537
AoxI GGCC 2 cut(s) 30, 468
ApaLI GTGCAC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 31, 199, 469
AsuHPI GGTGA 2 cut(s) 29, 335
AvaII GGWCC 1 cut(s) 199
AxyI CCTNAGG 1 cut(s) 369
BaeGI GKGCMC 1 cut(s) 83
Bbv12I GWGCWC 2 cut(s) 83, 297
BbvI GCAGC 1 cut(s) 18
BccI CCATC 4 cut(s) 372, 374, 425, 511
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 2 cut(s) 390, 527
BclI TGATCA 3 cut(s) 148, 325, 474
BcoDI GTCTC 3 cut(s) 379, 443, 511
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 171
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 390, 527
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 3 cut(s) 31, 199, 469
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 390, 527
BmsI GCATC 4 cut(s) 58, 127, 195, 315
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 3 cut(s) 202, 389, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 477
Bse1I ACTGG 1 cut(s) 76
Bse21I CCTNAGG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 390, 527
BseDI CCNNGG 3 cut(s) 202, 389, 441
BseGI GGATG 4 cut(s) 364, 427, 451, 522
BseLI CCNNNNNNNGG 2 cut(s) 370, 477
BseMII CTCAG 3 cut(s) 255, 349, 360
BseNI ACTGG 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 1 cut(s) 18
BshFI GGCC 2 cut(s) 32, 470
BsiHKAI GWGCWC 2 cut(s) 83, 297
BslFI GGGAC 2 cut(s) 212, 406
BslI CCNNNNNNNGG 2 cut(s) 370, 477
BsmAI GTCTC 3 cut(s) 379, 443, 511
BsmFI GGGAC 2 cut(s) 212, 406
BsnI GGCC 2 cut(s) 32, 470
Bso31I GGTCTC 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 83, 297
Bsp143I GATC 5 cut(s) 148, 312, 325, 474, 529
BspANI GGCC 2 cut(s) 32, 470
BspCNI CTCAG 3 cut(s) 256, 348, 361
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 200
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 320, 537
BspTNI GGTCTC 1 cut(s) 379
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 202, 389, 441
BssMI GATC 5 cut(s) 148, 312, 325, 474, 529
BssT1I CCWWGG 2 cut(s) 202, 441
Bst2UI CCWGG 2 cut(s) 390, 527
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 1 cut(s) 352
BstDEI CTNAG 4 cut(s) 218, 264, 335, 369
BstF5I GGATG 4 cut(s) 364, 427, 451, 522
BstKTI GATC 5 cut(s) 151, 315, 328, 477, 532
BstMAI GTCTC 3 cut(s) 379, 443, 511
BstMBI GATC 5 cut(s) 148, 312, 325, 474, 529
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 2 cut(s) 390, 527
BstNSI RCATGY 2 cut(s) 104, 456
BstSCI CCNGG 2 cut(s) 388, 525
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 18
Bsu36I CCTNAGG 1 cut(s) 369
BsuRI GGCC 2 cut(s) 32, 470
BtsCI GGATG 4 cut(s) 364, 427, 451, 522
BtsIMutI CAGTG 2 cut(s) 83, 261
BveI ACCTGC 1 cut(s) 171
CciI TCATGA 1 cut(s) 189
Cfr13I GGNCC 3 cut(s) 31, 199, 469
Csp6I GTAC 1 cut(s) 163
CviAII CATG 3 cut(s) 101, 190, 453
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 4 cut(s) 218, 264, 335, 369
DpnI GATC 5 cut(s) 150, 314, 327, 476, 531
DpnII GATC 5 cut(s) 148, 312, 325, 474, 529
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 202, 441
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 199
Eco81I CCTNAGG 1 cut(s) 369
EcoO109I RGGNCCY 2 cut(s) 199, 469
EcoRII CCWGG 2 cut(s) 388, 525
EcoT14I CCWWGG 2 cut(s) 202, 441
ErhI CCWWGG 2 cut(s) 202, 441
FaeI CATG 3 cut(s) 104, 193, 456
FaiI YATR 8 cut(s) 39, 41, 102, 127, 191, 330, 377, 454
FaqI GGGAC 2 cut(s) 212, 406
FatI CATG 3 cut(s) 100, 189, 452
FbaI TGATCA 3 cut(s) 148, 325, 474
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 4 cut(s) 351, 434, 458, 529
Fsp4HI GCNGC 1 cut(s) 7
FspBI CTAG 1 cut(s) 398
GluI GCNGC 1 cut(s) 7
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 2 cut(s) 32, 470
Hin1II CATG 3 cut(s) 104, 193, 456
HinfI GANTC 3 cut(s) 20, 214, 407
HphI GGTGA 2 cut(s) 29, 335
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 6 cut(s) 139, 190, 281, 310, 337, 368
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 81, 118, 186, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 4 cut(s) 218, 264, 335, 369
Hsp92II CATG 3 cut(s) 104, 193, 456
Ksp22I TGATCA 3 cut(s) 148, 325, 474
Kzo9I GATC 5 cut(s) 148, 312, 325, 474, 529
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 4 cut(s) 58, 127, 195, 315
MaeI CTAG 1 cut(s) 398
MalI GATC 5 cut(s) 150, 314, 327, 476, 531
MboI GATC 5 cut(s) 148, 312, 325, 474, 529
MboII GAAGA 1 cut(s) 339
MhlI GDGCHC 2 cut(s) 83, 297
MluCI AATT 2 cut(s) 340, 351
MlyI GAGTC 1 cut(s) 223
MnlI CCTC 9 cut(s) 8, 22, 39, 186, 261, 364, 460, 497, 507
MseI TTAA 2 cut(s) 105, 234
MspR9I CCNGG 2 cut(s) 390, 527
MvaI CCWGG 2 cut(s) 390, 527
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 5 cut(s) 148, 312, 325, 474, 529
NlaIII CATG 3 cut(s) 104, 193, 456
NlaIV GGNNCC 1 cut(s) 200
NspI RCATGY 2 cut(s) 104, 456
PagI TCATGA 1 cut(s) 189
PaqCI CACCTGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 2 cut(s) 20, 407
PfoI TCCNGGA 1 cut(s) 525
PkrI GCNGC 1 cut(s) 8
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PpuMI RGGWCCY 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 2 cut(s) 388, 525
PspGI CCWGG 2 cut(s) 388, 525
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 3 cut(s) 31, 199, 469
PspPPI RGGWCCY 1 cut(s) 199
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 105, 234
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 5 cut(s) 148, 312, 325, 474, 529
Sau96I GGNCC 3 cut(s) 31, 199, 469
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 390, 527
SduI GDGCHC 2 cut(s) 83, 297
SetI ASST 8 cut(s) 19, 185, 204, 279, 375, 482, 489, 544
SfaNI GCATC 4 cut(s) 58, 127, 195, 315
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
SpeI ACTAGT 1 cut(s) 397
Sse9I AATT 2 cut(s) 340, 351
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 2 cut(s) 388, 525
StyI CCWWGG 2 cut(s) 202, 441
TaaI ACNGT 1 cut(s) 361
TaqI TCGA 3 cut(s) 282, 405, 532
TasI AATT 2 cut(s) 340, 351
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 2 cut(s) 20, 407
Tru1I TTAA 2 cut(s) 105, 234
Tru9I TTAA 2 cut(s) 105, 234
TscAI CASTG 2 cut(s) 83, 268
TseI GCWGC 1 cut(s) 6
TspRI CASTG 2 cut(s) 83, 268
VneI GTGCAC 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 199
XceI RCATGY 2 cut(s) 104, 456
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.