Rorug01G0306000

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000297
Physical Location & Seq
Reverse (-)
42250245 .. 42252798
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0306000.1

Sequence Viewer

Length: 1053 bp
ATGGAGAAGAGGAAGAGTATGATGGGTAATTGGGAGCAAAAGATTCTAAAAAGTGAGTTAACCCAGGGGAAGGAGCTGGCAGAGAAGCTCCTGAACCATCTCCACCACTCTTCATCATCTCAAGAAGGAGAATTCTTGATTTCAAAGCTACTATCTTCATATGAAAAGGCACTCTCTGTGCTCACAAGGGATGTTGGTTCTGAAGGAGAGTCTAAGCAAATCCAAGTGTTGGACTCACCTTGTTCATTTGGGAATAGTAGTAGCCCATTGAGTGAGATCTCTGACCAAGATTGCAAGAACAAGAATGTCTTCAAGAAAAGGAAAACAATGCCCAAGTGGACTGAAGAAGTGAAGGTTTGCTCTGGAACAGGACAAGATGGCTCTCTTGATGATGGCTATAGTTGGAGAAAATATGGCCAAAAGGATATCCTTGGTGCTAATTACCCAAGAGGCTACTATCGATGCACACATAGAAACACAAAAGGTTGTCTAGCCACAAAGCAAGTTCAAAAATCAGATGCAGACCCAACAATCTTTATGGTAACTTATACAGGAGTACATACTTGTCACCTAGTCGTTCAGTTAAACGGAAAGCAGAATGTTTCTCTTAAACAAGAAGCAGGAACACCGAGACAGACACCAGAAAAATCGTTCAGTTTCGGGCTTAGAGTTAAAACTGAGAATTTGGACACTAGGGAGGGGGGAAAGGAGATATTTCCATCATTTTCCTTCTCTCCCACGCCAATAGAGTCTGAAAACAATGTGGTGGAAGACCATGTTTTCGCTGCAATTGAGAACGATTTCCTGGGTGGTAGCTACGGTCAAACATTCAGGACTCCGGCAACATTCGAATCAGACTACTTTGCGGCAGTGTCACCATGCCACTTTGGACTAGTAGGCAATGATGTGCAAACTTCAGATTCTGATCTCACTCATGAGATACTATCGGCCTCAACTTCTGTGGCCAACTCACCAATTGGGGATTTCGATTTCTCACTAGAGGATTTGGATTTTTACTCATTTGAAAACAACCCAGAATCTACTTTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

350

Amino Acids

38.94

Weight (kDa)

5.38

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 190 6.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42780 AT2G42780
fragaria_vesca FvH4_6g37640 FvH4_6g37640 FvH4_7g18970 FvH4_7g19430 FvH4_7g20490
malus_domestica MD04G1219900.v1.1 MD09G1156600.v1.1 MD11G1064800.v1.1 MD17G1143100.v1.1
prunus_persica Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1
pyrus_communis pycom09g07530 pycom11g05390 pycom12g21760 pycom17g13620
rosa_chinensis RchiOBHm_Chr1g0363261 RchiOBHm_Chr1g0363291 RchiOBHm_Chr1g0363311 RchiOBHm_Chr1g0363471 RchiOBHm_Chr1g0363891 RchiOBHm_Chr1g0377321 RchiOBHm_Chr2g0097481 RchiOBHm_Chr2g0101011 RchiOBHm_Chr2g0137701 RchiOBHm_Chr2g0150641 RchiOBHm_Chr2g0150701 RchiOBHm_Chr3g0476421 RchiOBHm_Chr5g0057921 RchiOBHm_Chr5g0057931 RchiOBHm_Chr5g0057951 RchiOBHm_Chr5g0057981 RchiOBHm_Chr5g0058001 RchiOBHm_Chr5g0058031 RchiOBHm_Chr5g0058051 RchiOBHm_Chr5g0058081 RchiOBHm_Chr5g0058101
rosa_laevigata RLG00000017036 RLG00000019670 RLG00000020502 RLG00000023768 RLG00000027563 RLG00000027575 RLG00000027577 RLG00000027578 RLG00000034860 RLG00000035193
rosa_multiflora Rmu_co8175074.1_g000001 Rmu_sc0000367.1_g000008 Rmu_sc0001862.1_g000004 Rmu_sc0001944.1_g000004 Rmu_sc0002483.1_g000004 Rmu_sc0004027.1_g000010 Rmu_sc0004458.1_g000001 Rmu_sc0004556.1_g000009 Rmu_sc0007226.1_g000008 Rmu_sc0011095.1_g000003 Rmu_sc0027954.1_g000002 Rmu_sc0028722.1_g000001
rosa_roxburghii Rroxscaffold_1G00022510 Rroxscaffold_1G00028200 Rroxscaffold_2G00097050 Rroxscaffold_2G00106910 Rroxscaffold_2G00106930 Rroxscaffold_2G00141650 Rroxscaffold_4G00293150 Rroxscaffold_4G00293320 Rroxscaffold_6G00405280
rosa_rugosa Rorug01G0305900 Rorug01G0306000 Rorug01G0306200 Rorug01G0307100 Rorug01G0307100 Rorug01G0307200 Rorug01G0310800 Rorug01G0402700 Rorug02G0095200 Rorug02G0344100.1 Rorug02G0421900 Rorug02G0422000 Rorug02G0422100 Rorug02G0422200 Rorug03G0154300 Rorug05G0310800 Rorug05G0310900 Rorug05G0337400
rosa_samantha Rh1AG314800 Rh1AG315000 Rh1AG316200 Rh1AG318700 Rh1AG420300 Rh1DG309100 Rh1DG309300 Rh1DG310600 Rh1DG313200 Rh1DG410200 Rh2AG142500 Rh2AG392100 Rh2AG481600 Rh2BG147600 Rh2BG399400 Rh2BG399600 Rh2BG493800 Rh2DG502800 Rh3BG237100 Rh3CG231600 Rh5BG390600 Rh5DG405200
rosa_wichuraiana Rw1G027900 Rw1G027910 Rw1G028000 Rw1G036850 Rw2G011180 Rw2G032110 Rw2G032120 Rw2G039370 Rw2G039510 Rw3G018680 Rw5G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 229
AciI CCGC 1 cut(s) 866
AcoI YGGCCR 2 cut(s) 415, 963
AcsI RAATTY 2 cut(s) 131, 682
AcuI CTGAAG 3 cut(s) 222, 363, 900
AfaI GTAC 1 cut(s) 558
AfiI CCNNNNNNNGG 2 cut(s) 70, 229
AgsI TTSAA 4 cut(s) 144, 313, 509, 1025
AhlI ACTAGT 1 cut(s) 892
AjnI CCWGG 2 cut(s) 63, 804
AluBI AGCT 4 cut(s) 76, 88, 148, 816
AluI AGCT 4 cut(s) 76, 88, 148, 816
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 625
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 3 cut(s) 415, 948, 963
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 2 cut(s) 131, 682
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
Asp700I GAANNNNTTC 2 cut(s) 308, 800
AsuHPI GGTGA 4 cut(s) 228, 560, 867, 963
AsuII TTCGAA 1 cut(s) 849
BalI TGGCCA 2 cut(s) 417, 965
BbsI GAAGAC 2 cut(s) 301, 777
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 772
BccI CCATC 5 cut(s) 16, 105, 371, 386, 727
BciT130I CCWGG 2 cut(s) 65, 806
BcoDI GTCTC 1 cut(s) 625
BcuI ACTAGT 1 cut(s) 892
BfaI CTAG 6 cut(s) 491, 572, 693, 893, 998, 1051
BfmI CTRYAG 1 cut(s) 397
BglII AGATCT 1 cut(s) 276
BisI GCNGC 2 cut(s) 786, 867
BlsI GCNGC 2 cut(s) 787, 868
Bme1390I CCNGG 2 cut(s) 65, 806
BmrFI CCNGG 2 cut(s) 65, 806
BmsI GCATC 2 cut(s) 452, 508
BpiI GAAGAC 2 cut(s) 301, 777
Bpu14I TTCGAA 1 cut(s) 849
BpuEI CTTGAG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 460
BsaBI GATNNNNATC 1 cut(s) 924
BsaJI CCNNGG 4 cut(s) 63, 64, 430, 805
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 229
Bse3DI GCAATG 1 cut(s) 907
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 2 cut(s) 65, 806
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 4 cut(s) 63, 64, 430, 805
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 2 cut(s) 70, 229
BseMI GCAATG 1 cut(s) 907
BseMII CTCAG 1 cut(s) 669
BseXI GCAGC 1 cut(s) 772
BshFI GGCC 3 cut(s) 417, 950, 965
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 839
BslI CCNNNNNNNGG 2 cut(s) 70, 229
BsmAI GTCTC 1 cut(s) 625
BsnI GGCC 3 cut(s) 417, 950, 965
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 276, 925
BspACI CCGC 1 cut(s) 866
BspANI GGCC 3 cut(s) 417, 950, 965
BspCNI CTCAG 1 cut(s) 670
BspDI ATCGAT 1 cut(s) 460
BspHI TCATGA 1 cut(s) 934
BspT104I TTCGAA 1 cut(s) 849
BsrDI GCAATG 1 cut(s) 907
BssECI CCNNGG 4 cut(s) 63, 64, 430, 805
BssMI GATC 2 cut(s) 276, 925
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 65, 806
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 3 cut(s) 2, 8, 115
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 3 cut(s) 213, 665, 678
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 2 cut(s) 279, 928
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 2 cut(s) 276, 925
BstNI CCWGG 2 cut(s) 65, 806
BstSCI CCNGG 2 cut(s) 63, 804
BstSFI CTRYAG 1 cut(s) 397
BstV1I GCAGC 1 cut(s) 772
BstV2I GAAGAC 2 cut(s) 301, 777
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 3 cut(s) 417, 950, 965
BsuTUI ATCGAT 1 cut(s) 460
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 1 cut(s) 876
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 934
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 557
CspCI CAANNNNNGTGG 2 cut(s) 942, 977
CviAII CATG 3 cut(s) 776, 879, 935
CviQI GTAC 1 cut(s) 557
DdeI CTNAG 3 cut(s) 213, 665, 678
DpnI GATC 2 cut(s) 278, 927
DpnII GATC 2 cut(s) 276, 925
EaeI YGGCCR 2 cut(s) 415, 963
Eam1104I CTCTTC 3 cut(s) 2, 8, 115
EarI CTCTTC 3 cut(s) 2, 8, 115
Eco130I CCWWGG 1 cut(s) 430
Eco32I GATATC 1 cut(s) 427
Eco57I CTGAAG 3 cut(s) 222, 363, 900
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 63, 804
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 3 cut(s) 779, 882, 938
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FatI CATG 3 cut(s) 775, 878, 934
FauNDI CATATG 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 786, 867
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 786, 867
FspBI CTAG 6 cut(s) 491, 572, 693, 893, 998, 1051
GluI GCNGC 2 cut(s) 786, 867
HaeIII GGCC 3 cut(s) 417, 950, 965
HapII CCGG 1 cut(s) 839
Hin1II CATG 3 cut(s) 779, 882, 938
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HinfI GANTC 8 cut(s) 43, 209, 233, 749, 835, 851, 920, 1037
HpaI GTTAAC 1 cut(s) 60
HpaII CCGG 1 cut(s) 839
HphI GGTGA 4 cut(s) 228, 560, 867, 963
Hpy166II GTNNAC 2 cut(s) 60, 339
Hpy188I TCNGA 7 cut(s) 202, 283, 517, 754, 856, 919, 925
Hpy188III TCNNGA 8 cut(s) 91, 122, 136, 313, 363, 386, 832, 935
Hpy8I GTNNAC 2 cut(s) 60, 339
HpyAV CCTTC 5 cut(s) 64, 119, 197, 346, 739
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4V TGCA 5 cut(s) 294, 465, 521, 788, 910
HpyF3I CTNAG 3 cut(s) 213, 665, 678
Hsp92II CATG 3 cut(s) 779, 882, 938
KspAI GTTAAC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 276, 925
LmnI GCTCC 3 cut(s) 34, 73, 93
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 452, 508
MaeI CTAG 6 cut(s) 491, 572, 693, 893, 998, 1051
MaeIII GTNAC 3 cut(s) 541, 566, 873
MalI GATC 2 cut(s) 278, 927
MboI GATC 2 cut(s) 276, 925
MboII GAAGA 7 cut(s) 19, 25, 102, 147, 301, 356, 782
MfeI CAATTG 2 cut(s) 789, 975
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 2 cut(s) 417, 965
MluCI AATT 6 cut(s) 28, 131, 439, 682, 789, 975
MluNI TGGCCA 2 cut(s) 417, 965
MlyI GAGTC 4 cut(s) 218, 227, 758, 829
MmeI TCCRAC 2 cut(s) 210, 383
MnlI CCTC 5 cut(s) 3, 443, 691, 961, 994
Mox20I TGGCCA 2 cut(s) 417, 965
MroXI GAANNNNTTC 2 cut(s) 308, 800
MscI TGGCCA 2 cut(s) 417, 965
MseI TTAA 4 cut(s) 59, 584, 609, 672
Msp20I TGGCCA 2 cut(s) 417, 965
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 2 cut(s) 65, 806
MunI CAATTG 2 cut(s) 789, 975
MvaI CCWGG 2 cut(s) 65, 806
NdeI CATATG 1 cut(s) 160
NdeII GATC 2 cut(s) 276, 925
NlaIII CATG 3 cut(s) 779, 882, 938
NmuCI GTSAC 2 cut(s) 566, 873
NspV TTCGAA 1 cut(s) 849
PagI TCATGA 1 cut(s) 934
PasI CCCWGGG 1 cut(s) 64
PdmI GAANNNNTTC 2 cut(s) 308, 800
PfeI GAWTC 4 cut(s) 43, 851, 920, 1037
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 2 cut(s) 787, 868
PleI GAGTC 4 cut(s) 217, 227, 757, 829
PpsI GAGTC 4 cut(s) 217, 227, 757, 829
Psp6I CCWGG 2 cut(s) 63, 804
PspGI CCWGG 2 cut(s) 63, 804
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 276
RsaI GTAC 1 cut(s) 558
RsaNI GTAC 1 cut(s) 557
SaqAI TTAA 4 cut(s) 59, 584, 609, 672
SatI GCNGC 2 cut(s) 786, 867
Sau3AI GATC 2 cut(s) 276, 925
SchI GAGTC 4 cut(s) 218, 227, 758, 829
ScrFI CCNGG 2 cut(s) 65, 806
SduI GDGCHC 1 cut(s) 183
SetI ASST 8 cut(s) 78, 90, 150, 241, 357, 487, 573, 818
SfaNI GCATC 2 cut(s) 452, 508
SfcI CTRYAG 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
SpeI ACTAGT 1 cut(s) 892
Sse9I AATT 6 cut(s) 28, 131, 439, 682, 789, 975
SsiI CCGC 1 cut(s) 866
SspMI CTAG 6 cut(s) 491, 572, 693, 893, 998, 1051
StyD4I CCNGG 2 cut(s) 63, 804
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 1 cut(s) 821
TaqI TCGA 3 cut(s) 460, 849, 987
TasI AATT 6 cut(s) 28, 131, 439, 682, 789, 975
TatI WGTACW 1 cut(s) 556
TauI GCSGC 1 cut(s) 869
TfiI GAWTC 4 cut(s) 43, 851, 920, 1037
Tru1I TTAA 4 cut(s) 59, 584, 609, 672
Tru9I TTAA 4 cut(s) 59, 584, 609, 672
TscAI CASTG 1 cut(s) 876
TseFI GTSAC 2 cut(s) 566, 873
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 2 cut(s) 566, 873
TspDTI ATGAA 4 cut(s) 102, 147, 177, 234
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 1 cut(s) 876
Van91I CCANNNNNTGG 1 cut(s) 229
XapI RAATTY 2 cut(s) 131, 682
XmnI GAANNNNTTC 2 cut(s) 308, 800
XspI CTAG 6 cut(s) 491, 572, 693, 893, 998, 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.