pycom12g21760

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000297
Physical Location & Seq
Reverse (-)
22875038 .. 22875739
702 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21760.1

Sequence Viewer

Length: 702 bp
ATGGATGTGGAAGCGAAAGCAGCAGCTCCTCCGTCTACGGTGGCTGATTTACTGCGCCACAACGGCGGTAATGTTAGCGACATAGATTTCAGTGTTCTGGAGAAAATCCTACCACAATGTACGGTCGGGGAGTTGAGGCAAGTAGAGAAGAGTAGCGGAGGTAGAGATTTGAGTCCAGTTACCGAAGAGTTGTGGCGGAAATTCTACGAACAGAAGTTCGGGCCTTTAAGGACTGATGCGGCGATCAAAAGGATGAAGAAGGAGAATGTGAGGTACAGATGGATGGAGCTGTACGATTCAAAGGAGAAGGAAGAACTCGACGAGGCCGAAAAGAAGGCTGCCGAGAGGTTGAAGAGGCGGTATAAAGAGGAAGCTTTGCGAAAACAAAGCCGGCGAGTTGTTATTTGCAAAGAGACAGAGGTTCCGTCATCGTCGTGGAACAAAAGAAAAAGAAGCGAGGATAAGGTTCCGTCGTCATCAAGTAACAAAGGAAGTAACACCGGCAGCTCCTGCGGTGGCGACGATGGCCCCGTCCGAAAGCGAAAGGAAAGCTTGATCATGAAGAAAGCGAGAAAAGATTTTCTCAACTGTATTGAGGTGAAAAATATTACAGCAATGGGGATGGTTAAATTGCAGAAGGGCTACAGTTCCAAGAAGGATTTGACATTAAAACGATGCGCCGTGCTCGCGGAAGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

234

Amino Acids

26.55

Weight (kDa)

9.51

Isoelectric Point (pI)

73.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 24 - 132 6.5e-18 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42780 AT2G42780
fragaria_vesca FvH4_6g37640 FvH4_6g37640 FvH4_7g18970 FvH4_7g19430 FvH4_7g20490
malus_domestica MD04G1219900.v1.1 MD09G1156600.v1.1 MD11G1064800.v1.1 MD17G1143100.v1.1
prunus_persica Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1
pyrus_communis pycom09g07530 pycom11g05390 pycom12g21760 pycom17g13620
rosa_chinensis RchiOBHm_Chr1g0363261 RchiOBHm_Chr1g0363291 RchiOBHm_Chr1g0363311 RchiOBHm_Chr1g0363471 RchiOBHm_Chr1g0363891 RchiOBHm_Chr1g0377321 RchiOBHm_Chr2g0097481 RchiOBHm_Chr2g0101011 RchiOBHm_Chr2g0137701 RchiOBHm_Chr2g0150641 RchiOBHm_Chr2g0150701 RchiOBHm_Chr3g0476421 RchiOBHm_Chr5g0057921 RchiOBHm_Chr5g0057931 RchiOBHm_Chr5g0057951 RchiOBHm_Chr5g0057981 RchiOBHm_Chr5g0058001 RchiOBHm_Chr5g0058031 RchiOBHm_Chr5g0058051 RchiOBHm_Chr5g0058081 RchiOBHm_Chr5g0058101
rosa_laevigata RLG00000017036 RLG00000019670 RLG00000020502 RLG00000023768 RLG00000027563 RLG00000027575 RLG00000027577 RLG00000027578 RLG00000034860 RLG00000035193
rosa_multiflora Rmu_co8175074.1_g000001 Rmu_sc0000367.1_g000008 Rmu_sc0001862.1_g000004 Rmu_sc0001944.1_g000004 Rmu_sc0002483.1_g000004 Rmu_sc0004027.1_g000010 Rmu_sc0004458.1_g000001 Rmu_sc0004556.1_g000009 Rmu_sc0007226.1_g000008 Rmu_sc0011095.1_g000003 Rmu_sc0027954.1_g000002 Rmu_sc0028722.1_g000001
rosa_roxburghii Rroxscaffold_1G00022510 Rroxscaffold_1G00028200 Rroxscaffold_2G00097050 Rroxscaffold_2G00106910 Rroxscaffold_2G00106930 Rroxscaffold_2G00141650 Rroxscaffold_4G00293150 Rroxscaffold_4G00293320 Rroxscaffold_6G00405280
rosa_rugosa Rorug01G0305900 Rorug01G0306000 Rorug01G0306200 Rorug01G0307100 Rorug01G0307100 Rorug01G0307200 Rorug01G0310800 Rorug01G0402700 Rorug02G0095200 Rorug02G0344100.1 Rorug02G0421900 Rorug02G0422000 Rorug02G0422100 Rorug02G0422200 Rorug03G0154300 Rorug05G0310800 Rorug05G0310900 Rorug05G0337400
rosa_samantha Rh1AG314800 Rh1AG315000 Rh1AG316200 Rh1AG318700 Rh1AG420300 Rh1DG309100 Rh1DG309300 Rh1DG310600 Rh1DG313200 Rh1DG410200 Rh2AG142500 Rh2AG392100 Rh2AG481600 Rh2BG147600 Rh2BG399400 Rh2BG399600 Rh2BG493800 Rh2DG502800 Rh3BG237100 Rh3CG231600 Rh5BG390600 Rh5DG405200
rosa_wichuraiana Rw1G027900 Rw1G027910 Rw1G028000 Rw1G036850 Rw2G011180 Rw2G032110 Rw2G032120 Rw2G039370 Rw2G039510 Rw3G018680 Rw5G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 35
AccII CGCG 1 cut(s) 689
AciI CCGC 7 cut(s) 66, 156, 196, 239, 358, 513, 689
AcsI RAATTY 1 cut(s) 200
AfaI GTAC 3 cut(s) 121, 275, 293
AgsI TTSAA 2 cut(s) 300, 352
AluBI AGCT 5 cut(s) 26, 289, 374, 507, 552
AluI AGCT 5 cut(s) 26, 289, 374, 507, 552
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 1 cut(s) 407
AlwNI CAGNNNCTG 1 cut(s) 510
AoxI GGCC 3 cut(s) 221, 324, 526
ApeKI GCWGC 4 cut(s) 20, 23, 338, 504
ApoI RAATTY 1 cut(s) 200
AspLEI GCGC 2 cut(s) 57, 680
AspS9I GGNCC 2 cut(s) 221, 527
AsuHPI GGTGA 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 4 cut(s) 32, 35, 325, 516
BccI CCATC 4 cut(s) 273, 277, 518, 616
BceAI ACGGC 2 cut(s) 79, 665
BclI TGATCA 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 643
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 5 cut(s) 21, 24, 240, 339, 505
BlsI GCNGC 5 cut(s) 22, 25, 241, 340, 506
BmgT120I GGNCC 2 cut(s) 221, 527
BmiI GGNNCC 3 cut(s) 423, 468, 529
BmsI GCATC 2 cut(s) 226, 665
BpmI CTGGAG 1 cut(s) 119
Bse118I RCCGGY 2 cut(s) 390, 500
Bse1I ACTGG 1 cut(s) 176
Bse3DI GCAATG 1 cut(s) 621
BseGI GGATG 4 cut(s) 10, 258, 288, 627
BseMI GCAATG 1 cut(s) 621
BseNI ACTGG 1 cut(s) 176
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 4 cut(s) 32, 35, 325, 516
Bsh1236I CGCG 1 cut(s) 689
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 223, 326, 528
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 2 cut(s) 391, 501
BsmAI GTCTC 1 cut(s) 407
BsnI GGCC 3 cut(s) 223, 326, 528
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 2 cut(s) 243, 555
BspACI CCGC 7 cut(s) 66, 156, 196, 239, 358, 513, 689
BspANI GGCC 3 cut(s) 223, 326, 528
BspFNI CGCG 1 cut(s) 689
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 3 cut(s) 423, 468, 529
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 2 cut(s) 390, 500
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 2 cut(s) 390, 500
BssMI GATC 2 cut(s) 243, 555
Bst4CI ACNGT 4 cut(s) 40, 124, 590, 647
Bst6I CTCTTC 4 cut(s) 143, 180, 347, 687
BstAPI GCANNNNNTGC 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 392, 687
BstF5I GGATG 4 cut(s) 10, 258, 288, 627
BstFNI CGCG 1 cut(s) 689
BstHHI GCGC 2 cut(s) 57, 680
BstKTI GATC 2 cut(s) 246, 558
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 2 cut(s) 243, 555
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 20, 63, 510, 525, 686
BstSFI CTRYAG 1 cut(s) 643
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 4 cut(s) 32, 35, 325, 516
BsuRI GGCC 3 cut(s) 223, 326, 528
BtsCI GGATG 4 cut(s) 10, 258, 288, 627
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 392, 687
CaiI CAGNNNCTG 1 cut(s) 510
CciI TCATGA 1 cut(s) 558
CfoI GCGC 2 cut(s) 57, 680
Cfr10I RCCGGY 2 cut(s) 390, 500
Cfr13I GGNCC 2 cut(s) 221, 527
Csp6I GTAC 3 cut(s) 120, 274, 292
CviAII CATG 1 cut(s) 559
CviQI GTAC 3 cut(s) 120, 274, 292
DpnI GATC 2 cut(s) 245, 557
DpnII GATC 2 cut(s) 243, 555
Eam1104I CTCTTC 4 cut(s) 143, 180, 347, 687
EarI CTCTTC 4 cut(s) 143, 180, 347, 687
EciI GGCGGA 1 cut(s) 211
FaeI CATG 1 cut(s) 562
FaiI YATR 3 cut(s) 83, 363, 560
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FatI CATG 1 cut(s) 558
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 5 cut(s) 21, 24, 240, 339, 505
FokI GGATG 4 cut(s) 17, 265, 295, 634
Fsp4HI GCNGC 5 cut(s) 21, 24, 240, 339, 505
GlaI GCGC 2 cut(s) 56, 679
GluI GCNGC 5 cut(s) 21, 24, 240, 339, 505
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 3 cut(s) 223, 326, 528
HapII CCGG 2 cut(s) 391, 501
HhaI GCGC 2 cut(s) 57, 680
Hin1II CATG 1 cut(s) 562
Hin6I GCGC 2 cut(s) 55, 678
HinP1I GCGC 2 cut(s) 55, 678
HindIII AAGCTT 2 cut(s) 372, 550
HinfI GANTC 2 cut(s) 172, 296
HpaII CCGG 2 cut(s) 391, 501
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 1 cut(s) 536
Hpy188III TCNNGA 2 cut(s) 98, 559
Hpy8I GTNNAC 1 cut(s) 36
Hpy99I CGWCG 4 cut(s) 323, 436, 475, 524
HpyAV CCTTC 5 cut(s) 253, 301, 328, 631, 649
HpyCH4III ACNGT 4 cut(s) 40, 124, 590, 647
HpyCH4V TGCA 2 cut(s) 408, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 63, 510, 525, 686
Hsp92II CATG 1 cut(s) 562
HspAI GCGC 2 cut(s) 55, 678
KroI GCCGGC 1 cut(s) 390
KroNI GCCGGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 2 cut(s) 243, 555
LmnI GCTCC 3 cut(s) 31, 286, 512
LpnPI CCDG 5 cut(s) 83, 189, 404, 514, 523
Lsp1109I GCAGC 4 cut(s) 32, 35, 325, 516
LweI GCATC 2 cut(s) 226, 665
MaeIII GTNAC 3 cut(s) 178, 482, 494
MalI GATC 2 cut(s) 245, 557
MboI GATC 2 cut(s) 243, 555
MboII GAAGA 6 cut(s) 160, 197, 268, 323, 364, 574
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 2 cut(s) 200, 629
MlyI GAGTC 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 390
MseI TTAA 3 cut(s) 227, 627, 668
MslI CAYNNNNRTG 1 cut(s) 433
MspI CCGG 2 cut(s) 391, 501
MvnI CGCG 1 cut(s) 689
MwoI GCNNNNNNNGC 5 cut(s) 20, 63, 510, 525, 686
NaeI GCCGGC 1 cut(s) 392
NdeII GATC 2 cut(s) 243, 555
NgoMIV GCCGGC 1 cut(s) 390
NlaIII CATG 1 cut(s) 562
NlaIV GGNNCC 3 cut(s) 423, 468, 529
NmeAIII GCCGAG 1 cut(s) 367
PagI TCATGA 1 cut(s) 558
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 528
PdiI GCCGGC 1 cut(s) 392
PfeI GAWTC 1 cut(s) 296
PkrI GCNGC 5 cut(s) 22, 25, 241, 340, 506
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 423, 468, 529
PspPI GGNCC 2 cut(s) 221, 527
PstNI CAGNNNCTG 1 cut(s) 510
RsaI GTAC 3 cut(s) 121, 275, 293
RsaNI GTAC 3 cut(s) 120, 274, 292
RseI CAYNNNNRTG 1 cut(s) 433
SaqAI TTAA 3 cut(s) 227, 627, 668
SatI GCNGC 5 cut(s) 21, 24, 240, 339, 505
Sau3AI GATC 2 cut(s) 243, 555
Sau96I GGNCC 2 cut(s) 221, 527
SchI GAGTC 1 cut(s) 181
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 2 cut(s) 226, 665
SfcI CTRYAG 1 cut(s) 643
SmiMI CAYNNNNRTG 1 cut(s) 433
Sse9I AATT 2 cut(s) 200, 629
SsiI CCGC 7 cut(s) 66, 156, 196, 239, 358, 513, 689
SspI AATATT 1 cut(s) 607
TaaI ACNGT 4 cut(s) 40, 124, 590, 647
TaqI TCGA 1 cut(s) 318
TasI AATT 2 cut(s) 200, 629
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 227, 627, 668
Tru9I TTAA 3 cut(s) 227, 627, 668
TscAI CASTG 1 cut(s) 97
TseI GCWGC 4 cut(s) 20, 23, 338, 504
TspDTI ATGAA 2 cut(s) 269, 575
TspGWI ACGGA 3 cut(s) 21, 414, 459
TspRI CASTG 1 cut(s) 97
XapI RAATTY 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.