Rorug05G0337400

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000297
Physical Location & Seq
Reverse (-)
40970280 .. 40973487
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0337400.1

Sequence Viewer

Length: 2874 bp
ATGCATCCATCTAAGGCACCGGGCCCAGATGGTTTTGCTCCATGTTTTTATCAAGCCTTCTGGGATTTGGTTGGAAATGATGTTGTTAGGGCAGTTAGAGATTTTGTGGGTTCGGACGATAAACTGAAAGTGGTGAATGAAACCGTAGTCGCTTTAATTCCAAAGGTAGACACTCCTCAGCATATGCATCAGTTGAGGCCTATTAGCCTCTGCAACGTGCTCTACAAAATTGGGTCGAAGGTCCTAGCTAACCGTTTGAAGCCGCTTTTAAATGAGGTAATCTCGCCCTTCCAAAGTGCATTCGTACCTGGGAGATTAATTTCGGATAATTCTTTGTTGGCATTTGAGGTCTCTCATTGTCTAAAAAGGCGTAGAAGTGGAAAAAAAGCTTATTGTGCGTTGAAACTTGATATGAGTAAGGCATATGACAGAGTCGAGTGGGAGTTTCTGGAAGCAGTTATGTTGAAGATGGGGTTCTGTAATGTGTGGGTTAATTGGATTATGGCTTCTGTTCGTACAGTCTCCTACTCTTTCATTGTTAATGGTGACCCTAGGGGCCGAATTATTCCATCCAGGGGTCTACGACAAGGAGATGCTATTTCACCTTATCTTTTTCTTCTGGTTGCTAAGGCCTTATCGAGGTTAATTATGGTTGCAGAAAGGGATGACAGCTTTCACGGTGTGGAGGTGTGTAGGGGTGCACCAAGTATTAGCCATTTATTTTTTGCAGACGATTCCTTTATATTTTTCAAAGCTGAGGCAGCAGAATGTATGGTTCTGAAGGGGATTCTCCAGGACAATGAAAATGCATCTGGCCAGCAGGTTAACCTTCAAAAGAGCTGCATCTCATTTAGTAAGAATGTGGCGTTGGACTGTCAAGAGGAACTTGCATCATTATTGGGTGTTACAAGGGTAGAGAAGCATGATAAATACCTTGGATTGCCAACAGAGATCAGTTATTCAAAGGAGGAAGCGTTTGCATTTCTGAATGAAAAGATTAGAGCTCGTACACAAGGTTGGAGAGAAAAAACATTATCGGTCGCTGGAAGAGAAGTTTTGATTAAAGCAGTTGCACAAGCCATTCCCTCTTATGTGATGAGTTGCTTCGAATTACCCAAACATTTGTGTAATGAGATGCAACAATTAATGGCTCGGTTTTGGTGGGGAGGATCAGAGGATGAGAAGAAGATTCATTGGGTCTCTTGGGAGAAATTATGTCGCCCAAAAGTGGAGGGAGGTATGGGTTTTCGGAACATGCATTGCTTTAACCTAGCTCTCTTGGCTAAACAGGGGTGGAGGTTGATACAAAAGCCAAATTCATTAATTGCTCGTGTTTTGAAGGCCAAATATTTCCCCCATTGTTCTTTCATGGCAGCGGAGATGAAGCAGGGTTCGTCATATACATGGCGTAGTATTATGGCGGGACGGGAAGTGCTACGTCAAGGAGTCCGTTATCAAGTTGGAGATGGGAGTACTATTTCTTTGTGGCATGACCCCTGGATGCCCATGCCTAGTTCTTTTAGACCGTTCTCTCCTATAATGGAGGGTACTGAAAATTGGGTAGTTGCAGATGTCATAGATGCCGACGATAAGGAGTGGGTTGTTGATGTTCTGAGTGAGCTTTTTACGGAGATGGAGGTCACTAAGATAGCAGCTATTCCCTTGAGCATTAGACCGGCAGCAGACCGAATGGTATGGCATTATGATGGCAAGGGTGTGTACAATGTAAGAAGTGGGTATCATGTAATGGTTAATTCCTTGAGAAGTCATGCATGGGCGTCAACATCTAGCTTAATTGCTGGTGGTCAATTAAGGAGCTACTGGAACAAATTGTGGCAAGCTAATGTGCCACCAAAAGTCAAAGTCTTCACATGGCGCTTGTTGAATGGAGCCCTTCCCACCAGGTCTGCACTTGTTTCACGTAACATTCCTTTGCCTGATATTACATGTGTTTTCTGCTGCAATTCGGTGGAGACCGAGATGCACTTATTTAAAGATTGCATGGCACTCCAATGCTTTTGGAATTTCTGTCCATTAAAATTAAAGCCTAAGACTCATGTTGCTGCTGATGTGAAGGCCTGGGTCTTTGATATGATGGATCTATTGTGTTATGGGCAGTGTGATTTTTTTCTTTTGAGCCTATGGCTGATTTGGTGTGAGAGAAATAACTTAGTCTGGAAGGGTGGTAATTTCCAACCCATGCATATGATCCAGTGGTGTAAACAGCAGTTGGAGGACTTTCAGCTATACCATCCAAAGGCTTCAAGGAAAAAGAAAAAGCGTTTGACTAAATGGGAATGTCCTCCTTCAGGAAGACTGAAAATTAATGTTGATGGGGCGTTCAGAGCTGATTGTGGAAGTGGTGGTATTGGGGTGGTGGTCAGAGATGAATGGGGCATGGGTATCGCGGCAATTGCAAGGCCTTTTCTGCATGCGCACTCGGCTATTAATATGGAAGCCGAGGCGTGTAGAGCTGGTCTTCTTCTTGGTATACACCAAGGTTGGACGGAAATTGACATTGAAAGCGACTCTGCCATTCTGACTGCTGCTCTCAATAGCAAGGAGGTGAATTATTCGGAGGTAAGTCGAGTTTTGGATGATTGTAAAGATTACTTATCTGCTTTTCAATCAGTTCGAATTCGGCATATCTACCGTGAAGCAAATGGTGTTGCACATAGGCTTGCTCACCTTGCTAGTATTGATTGGTTAGATGATGTTTGGTTAGATGAGACTCCTGCTATTATTCAGGATGTACTCTACGAGGATTATAGTCATTGTTCTAATGTAGCACGGGGTTCAGGTTTTATGTCCCCCCCGTTGCAAAATATTATTTCAATAAATGGAACCAGGCATGGGGCTGAGCCTCCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

957

Amino Acids

108.28

Weight (kDa)

8.54

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 53 - 305 1e-28 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 603 - 674 5.6e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 776 - 897 1.3e-23 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42780 AT2G42780
fragaria_vesca FvH4_6g37640 FvH4_6g37640 FvH4_7g18970 FvH4_7g19430 FvH4_7g20490
malus_domestica MD04G1219900.v1.1 MD09G1156600.v1.1 MD11G1064800.v1.1 MD17G1143100.v1.1
prunus_persica Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1 Prupe.3G169700_v2.0.a1
pyrus_communis pycom09g07530 pycom11g05390 pycom12g21760 pycom17g13620
rosa_chinensis RchiOBHm_Chr1g0363261 RchiOBHm_Chr1g0363291 RchiOBHm_Chr1g0363311 RchiOBHm_Chr1g0363471 RchiOBHm_Chr1g0363891 RchiOBHm_Chr1g0377321 RchiOBHm_Chr2g0097481 RchiOBHm_Chr2g0101011 RchiOBHm_Chr2g0137701 RchiOBHm_Chr2g0150641 RchiOBHm_Chr2g0150701 RchiOBHm_Chr3g0476421 RchiOBHm_Chr5g0057921 RchiOBHm_Chr5g0057931 RchiOBHm_Chr5g0057951 RchiOBHm_Chr5g0057981 RchiOBHm_Chr5g0058001 RchiOBHm_Chr5g0058031 RchiOBHm_Chr5g0058051 RchiOBHm_Chr5g0058081 RchiOBHm_Chr5g0058101
rosa_laevigata RLG00000017036 RLG00000019670 RLG00000020502 RLG00000023768 RLG00000027563 RLG00000027575 RLG00000027577 RLG00000027578 RLG00000034860 RLG00000035193
rosa_multiflora Rmu_co8175074.1_g000001 Rmu_sc0000367.1_g000008 Rmu_sc0001862.1_g000004 Rmu_sc0001944.1_g000004 Rmu_sc0002483.1_g000004 Rmu_sc0004027.1_g000010 Rmu_sc0004458.1_g000001 Rmu_sc0004556.1_g000009 Rmu_sc0007226.1_g000008 Rmu_sc0011095.1_g000003 Rmu_sc0027954.1_g000002 Rmu_sc0028722.1_g000001
rosa_roxburghii Rroxscaffold_1G00022510 Rroxscaffold_1G00028200 Rroxscaffold_2G00097050 Rroxscaffold_2G00106910 Rroxscaffold_2G00106930 Rroxscaffold_2G00141650 Rroxscaffold_4G00293150 Rroxscaffold_4G00293320 Rroxscaffold_6G00405280
rosa_rugosa Rorug01G0305900 Rorug01G0306000 Rorug01G0306200 Rorug01G0307100 Rorug01G0307100 Rorug01G0307200 Rorug01G0310800 Rorug01G0402700 Rorug02G0095200 Rorug02G0344100.1 Rorug02G0421900 Rorug02G0422000 Rorug02G0422100 Rorug02G0422200 Rorug03G0154300 Rorug05G0310800 Rorug05G0310900 Rorug05G0337400
rosa_samantha Rh1AG314800 Rh1AG315000 Rh1AG316200 Rh1AG318700 Rh1AG420300 Rh1DG309100 Rh1DG309300 Rh1DG310600 Rh1DG313200 Rh1DG410200 Rh2AG142500 Rh2AG392100 Rh2AG481600 Rh2BG147600 Rh2BG399400 Rh2BG399600 Rh2BG493800 Rh2DG502800 Rh3BG237100 Rh3CG231600 Rh5BG390600 Rh5DG405200
rosa_wichuraiana Rw1G027900 Rw1G027910 Rw1G028000 Rw1G036850 Rw2G011180 Rw2G032110 Rw2G032120 Rw2G039370 Rw2G039510 Rw3G018680 Rw5G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2436
Acc36I ACCTGC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 16
AccI GTMKAC 3 cut(s) 168, 580, 2491
AccII CGCG 1 cut(s) 2408
AciI CCGC 4 cut(s) 263, 1376, 1421, 2408
AclWI GGATC 3 cut(s) 1177, 2106, 2203
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 3 cut(s) 1315, 2023, 2637
AcuI CTGAAG 2 cut(s) 800, 2292
AcyI GRCGYC 1 cut(s) 1778
AdeI CACNNNGTG 1 cut(s) 682
AfaI GTAC 7 cut(s) 306, 517, 1009, 1474, 1549, 1721, 2754
AfiI CCNNNNNNNGG 6 cut(s) 575, 639, 1228, 2257, 2309, 2853
AflIII ACRYGT 1 cut(s) 1946
AjnI CCWGG 7 cut(s) 307, 572, 792, 1496, 1901, 2078, 2846
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
Alw21I GWGCWC 3 cut(s) 222, 703, 1006
Alw26I GTCTC 5 cut(s) 355, 526, 1204, 1967, 2723
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 3 cut(s) 1177, 2106, 2203
AoxI GGCC 8 cut(s) 22, 197, 556, 630, 814, 1341, 2076, 2420
ApaI GGGCCC 1 cut(s) 26
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 8 cut(s) 761, 840, 1373, 1652, 1679, 1959, 2063, 2546
ApoI RAATTY 3 cut(s) 1315, 2023, 2637
AseI ATTAAT 5 cut(s) 317, 1145, 1322, 2325, 2448
AspA2I CCTAGG 1 cut(s) 551
AspLEI GCGC 2 cut(s) 1878, 2437
AspS9I GGNCC 4 cut(s) 22, 23, 241, 556
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 5 cut(s) 145, 557, 594, 2578, 2678
AsuII TTCGAA 2 cut(s) 1107, 2635
AvaII GGWCC 1 cut(s) 241
AvrII CCTAGG 1 cut(s) 551
BaeGI GKGCMC 2 cut(s) 26, 703
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 3 cut(s) 26, 1006, 1894
BauI CACGAG 1 cut(s) 1329
BbsI GAAGAC 3 cut(s) 1858, 2320, 2471
Bbv12I GWGCWC 3 cut(s) 222, 703, 1006
BbvCI CCTCAGC 2 cut(s) 177, 756
BbvI GCAGC 8 cut(s) 773, 827, 1385, 1664, 1691, 1946, 2050, 2533
BcgI CGANNNNNNTGC 2 cut(s) 2386, 2420
BciT130I CCWGG 7 cut(s) 309, 574, 794, 1498, 1903, 2080, 2848
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 5 cut(s) 355, 526, 1204, 1967, 2723
BfaI CTAG 7 cut(s) 245, 552, 1271, 1512, 1788, 2694, 2872
BfoI RGCGCY 1 cut(s) 1879
BfuAI ACCTGC 1 cut(s) 811
BlnI CCTAGG 1 cut(s) 551
BlpI GCTNAGC 1 cut(s) 2859
BmcAI AGTACT 1 cut(s) 1474
Bme1390I CCNGG 8 cut(s) 21, 309, 574, 794, 1498, 1903, 2080, 2848
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 22, 23, 241, 556
BmiI GGNNCC 5 cut(s) 18, 24, 557, 1891, 2845
BmrFI CCNGG 8 cut(s) 21, 309, 574, 794, 1498, 1903, 2080, 2848
BpiI GAAGAC 3 cut(s) 1858, 2320, 2471
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 3 cut(s) 177, 627, 756
Bpu1102I GCTNAGC 1 cut(s) 2859
Bpu14I TTCGAA 2 cut(s) 1107, 2635
BpuEI CTTGAG 2 cut(s) 1684, 1780
BpuMI CCSGG 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 1922
BsaHI GRCGYC 1 cut(s) 1778
BsaI GGTCTC 3 cut(s) 355, 1204, 1967
BsaJI CCNNGG 8 cut(s) 308, 551, 573, 934, 1496, 2079, 2460, 2497
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 6 cut(s) 575, 639, 1228, 2257, 2309, 2853
Bse118I RCCGGY 1 cut(s) 1675
Bse1I ACTGG 2 cut(s) 1826, 2212
Bse3DI GCAATG 1 cut(s) 1258
BseBI CCWGG 7 cut(s) 309, 574, 794, 1498, 1903, 2080, 2848
BseDI CCNNGG 8 cut(s) 308, 551, 573, 934, 1496, 2079, 2460, 2497
BseGI GGATG 8 cut(s) 4, 569, 670, 1183, 1506, 2251, 2602, 2755
BseLI CCNNNNNNNGG 6 cut(s) 575, 639, 1228, 2257, 2309, 2853
BseMI GCAATG 1 cut(s) 1258
BseMII CTCAG 4 cut(s) 191, 747, 1604, 2850
BseNI ACTGG 2 cut(s) 1826, 2212
BseRI GAGGAG 1 cut(s) 165
BseSI GKGCMC 2 cut(s) 26, 703
BseXI GCAGC 8 cut(s) 773, 827, 1385, 1664, 1691, 1946, 2050, 2533
BseYI CCCAGC 1 cut(s) 2867
BsgI GTGCAG 1 cut(s) 1893
Bsh1236I CGCG 1 cut(s) 2408
Bsh1285I CGRYCG 1 cut(s) 1041
BshFI GGCC 8 cut(s) 24, 199, 558, 632, 816, 1343, 2078, 2422
BshNI GGYRCC 1 cut(s) 16
BsiEI CGRYCG 1 cut(s) 1041
BsiHKAI GWGCWC 3 cut(s) 222, 703, 1006
BsiSI CCGG 2 cut(s) 20, 1676
BslFI GGGAC 2 cut(s) 1437, 2794
BslI CCNNNNNNNGG 6 cut(s) 575, 639, 1228, 2257, 2309, 2853
BsmAI GTCTC 5 cut(s) 355, 526, 1204, 1967, 2723
BsmFI GGGAC 2 cut(s) 1437, 2794
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 8 cut(s) 24, 199, 558, 632, 816, 1343, 2078, 2422
Bso31I GGTCTC 3 cut(s) 355, 1204, 1967
Bsp119I TTCGAA 2 cut(s) 1107, 2635
Bsp120I GGGCCC 1 cut(s) 22
Bsp1286I GDGCHC 5 cut(s) 26, 222, 703, 1006, 1894
Bsp1407I TGTACA 1 cut(s) 1719
Bsp143I GATC 4 cut(s) 951, 1169, 2098, 2208
Bsp1720I GCTNAGC 1 cut(s) 2859
BspACI CCGC 4 cut(s) 263, 1376, 1421, 2408
BspANI GGCC 8 cut(s) 24, 199, 558, 632, 816, 1343, 2078, 2422
BspCNI CTCAG 4 cut(s) 190, 748, 1605, 2851
BspFNI CGCG 1 cut(s) 2408
BspLI GGNNCC 5 cut(s) 18, 24, 557, 1891, 2845
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 1177, 2106, 2203
BspT104I TTCGAA 2 cut(s) 1107, 2635
BspT107I GGYRCC 1 cut(s) 16
BspTNI GGTCTC 3 cut(s) 355, 1204, 1967
BsrDI GCAATG 1 cut(s) 1258
BsrFI RCCGGY 1 cut(s) 1675
BsrGI TGTACA 1 cut(s) 1719
BsrI ACTGG 2 cut(s) 1826, 2212
BssAI RCCGGY 1 cut(s) 1675
BssECI CCNNGG 8 cut(s) 308, 551, 573, 934, 1496, 2079, 2460, 2497
BssMI GATC 4 cut(s) 951, 1169, 2098, 2208
BssNAI GTATAC 1 cut(s) 2492
BssNI GRCGYC 1 cut(s) 1778
BssSI CACGAG 1 cut(s) 1329
BssT1I CCWWGG 3 cut(s) 551, 934, 2497
Bst1107I GTATAC 1 cut(s) 2492
Bst2BI CACGAG 1 cut(s) 1329
Bst2UI CCWGG 7 cut(s) 309, 574, 794, 1498, 1903, 2080, 2848
Bst4CI ACNGT 7 cut(s) 145, 254, 520, 680, 875, 1527, 2654
Bst6I CTCTTC 1 cut(s) 1042
BstACI GRCGYC 1 cut(s) 1778
BstAUI TGTACA 1 cut(s) 1719
BstBAI YACGTR 1 cut(s) 1922
BstBI TTCGAA 2 cut(s) 1107, 2635
BstC8I GCNNGC 4 cut(s) 818, 1839, 2433, 2682
BstDEI CTNAG 9 cut(s) 12, 177, 627, 756, 1613, 1644, 2049, 2170, 2859
BstEII GGTNACC 1 cut(s) 545
BstENI CCTNNNNNAGG 2 cut(s) 637, 2307
BstF5I GGATG 8 cut(s) 4, 569, 670, 1183, 1506, 2251, 2602, 2755
BstFNI CGCG 1 cut(s) 2408
BstH2I RGCGCY 1 cut(s) 1879
BstHHI GCGC 2 cut(s) 1878, 2437
BstKTI GATC 4 cut(s) 954, 1172, 2101, 2211
BstMAI GTCTC 5 cut(s) 355, 526, 1204, 1967, 2723
BstMBI GATC 4 cut(s) 951, 1169, 2098, 2208
BstMCI CGRYCG 1 cut(s) 1041
BstMWI GCNNNNNNNGC 9 cut(s) 395, 761, 1280, 2345, 2414, 2428, 2441, 2471, 2690
BstNI CCWGG 7 cut(s) 309, 574, 794, 1498, 1903, 2080, 2848
BstNSI RCATGY 3 cut(s) 1258, 1950, 2435
BstPI GGTNACC 1 cut(s) 545
BstSCI CCNGG 8 cut(s) 19, 307, 572, 792, 1496, 1901, 2078, 2846
BstSLI GKGCMC 2 cut(s) 26, 703
BstUI CGCG 1 cut(s) 2408
BstV1I GCAGC 8 cut(s) 773, 827, 1385, 1664, 1691, 1946, 2050, 2533
BstV2I GAAGAC 3 cut(s) 1858, 2320, 2471
BstX2I RGATCY 1 cut(s) 2098
BstYI RGATCY 1 cut(s) 2098
BstZ17I GTATAC 1 cut(s) 2492
BsuRI GGCC 8 cut(s) 24, 199, 558, 632, 816, 1343, 2078, 2422
BtsCI GGATG 8 cut(s) 4, 569, 670, 1183, 1506, 2251, 2602, 2755
BtsI GCAGTG 1 cut(s) 2123
BtsIMutI CAGTG 2 cut(s) 2123, 2219
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 4 cut(s) 818, 1839, 2433, 2682
CfoI GCGC 2 cut(s) 1878, 2437
Cfr10I RCCGGY 1 cut(s) 1675
Cfr13I GGNCC 4 cut(s) 22, 23, 241, 556
CseI GACGC 1 cut(s) 1767
CsiI ACCWGGT 1 cut(s) 1901
Csp6I GTAC 7 cut(s) 305, 516, 1008, 1473, 1548, 1720, 2753
CviQI GTAC 7 cut(s) 305, 516, 1008, 1473, 1548, 1720, 2753
DdeI CTNAG 9 cut(s) 12, 177, 627, 756, 1613, 1644, 2049, 2170, 2859
DpnI GATC 4 cut(s) 953, 1171, 2100, 2210
DpnII GATC 4 cut(s) 951, 1169, 2098, 2208
DraI TTTAAA 2 cut(s) 270, 1993
DraIII CACNNNGTG 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 1 cut(s) 1042
EarI CTCTTC 1 cut(s) 1042
Ecl136II GAGCTC 1 cut(s) 1004
Eco130I CCWWGG 3 cut(s) 551, 934, 2497
Eco147I AGGCCT 4 cut(s) 199, 632, 2078, 2422
Eco24I GRGCYC 3 cut(s) 26, 1006, 1894
Eco31I GGTCTC 3 cut(s) 355, 1204, 1967
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 1004
Eco57I CTGAAG 2 cut(s) 800, 2292
Eco91I GGTNACC 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 1004
EcoNI CCTNNNNNAGG 2 cut(s) 637, 2307
EcoO109I RGGNCCY 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 2637
EcoRII CCWGG 7 cut(s) 307, 572, 792, 1496, 1901, 2078, 2846
EcoT14I CCWWGG 3 cut(s) 551, 934, 2497
EcoT22I ATGCAT 6 cut(s) 6, 189, 811, 1260, 1774, 2205
EcoT38I GRGCYC 3 cut(s) 26, 1006, 1894
ErhI CCWWGG 3 cut(s) 551, 934, 2497
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 2 cut(s) 1437, 2794
FauI CCCGC 1 cut(s) 1414
FauNDI CATATG 3 cut(s) 183, 424, 2205
FblI GTMKAC 3 cut(s) 168, 580, 2491
FokI GGATG 7 cut(s) 556, 677, 1190, 1513, 2238, 2609, 2762
FriOI GRGCYC 3 cut(s) 26, 1006, 1894
FspAI RTGCGCAY 1 cut(s) 2436
FspBI CTAG 7 cut(s) 245, 552, 1271, 1512, 1788, 2694, 2872
FspI TGCGCA 1 cut(s) 2436
GlaI GCGC 2 cut(s) 1877, 2436
GsaI CCCAGC 1 cut(s) 2871
GsuI CTGGAG 1 cut(s) 776
HaeII RGCGCY 1 cut(s) 1879
HaeIII GGCC 8 cut(s) 24, 199, 558, 632, 816, 1343, 2078, 2422
HapII CCGG 2 cut(s) 20, 1676
HgaI GACGC 1 cut(s) 1767
HhaI GCGC 2 cut(s) 1878, 2437
Hin1I GRCGYC 1 cut(s) 1778
Hin6I GCGC 2 cut(s) 1876, 2435
HinP1I GCGC 2 cut(s) 1876, 2435
HincII GTYRAC 2 cut(s) 826, 1782
HindII GTYRAC 2 cut(s) 826, 1782
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 8 cut(s) 432, 734, 787, 1189, 1446, 2053, 2528, 2731
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 2 cut(s) 20, 1676
HphI GGTGA 5 cut(s) 145, 557, 594, 2578, 2678
Hpy166II GTNNAC 9 cut(s) 169, 581, 701, 826, 1010, 1720, 1782, 2222, 2492
Hpy188III TCNNGA 5 cut(s) 449, 878, 2176, 2310, 2747
Hpy8I GTNNAC 9 cut(s) 169, 581, 701, 826, 1010, 1720, 1782, 2222, 2492
Hpy99I CGWCG 1 cut(s) 1589
HpyCH4III ACNGT 7 cut(s) 145, 254, 520, 680, 875, 1527, 2654
HpyCH4IV ACGT 3 cut(s) 216, 1438, 1921
HpyF10VI GCNNNNNNNGC 9 cut(s) 395, 761, 1280, 2345, 2414, 2428, 2441, 2471, 2690
HpyF3I CTNAG 9 cut(s) 12, 177, 627, 756, 1613, 1644, 2049, 2170, 2859
HpySE526I ACGT 3 cut(s) 216, 1438, 1921
Hsp92I GRCGYC 1 cut(s) 1778
HspAI GCGC 2 cut(s) 1876, 2435
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 4 cut(s) 951, 1169, 2098, 2208
LmnI GCTCC 3 cut(s) 43, 1815, 1889
Lsp1109I GCAGC 8 cut(s) 773, 827, 1385, 1664, 1691, 1946, 2050, 2533
MabI ACCWGGT 1 cut(s) 1901
MaeI CTAG 7 cut(s) 245, 552, 1271, 1512, 1788, 2694, 2872
MaeII ACGT 3 cut(s) 216, 1438, 1921
MaeIII GTNAC 4 cut(s) 545, 904, 1639, 1922
MalI GATC 4 cut(s) 953, 1171, 2100, 2210
MboI GATC 4 cut(s) 951, 1169, 2098, 2208
MboII GAAGA 9 cut(s) 478, 608, 1059, 1195, 1198, 1858, 2325, 2471, 2474
MfeI CAATTG 1 cut(s) 2412
MflI RGATCY 1 cut(s) 2098
MhlI GDGCHC 5 cut(s) 26, 222, 703, 1006, 1894
MlsI TGGCCA 1 cut(s) 816
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 5 cut(s) 441, 1455, 2047, 2522, 2725
MmeI TCCRAC 7 cut(s) 52, 849, 998, 1441, 2211, 2218, 2483
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 6 cut(s) 6, 189, 811, 1260, 1774, 2205
MscI TGGCCA 1 cut(s) 816
MslI CAYNNNNRTG 4 cut(s) 1402, 1704, 2011, 2204
Msp20I TGGCCA 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 1376
MspI CCGG 2 cut(s) 20, 1676
MspR9I CCNGG 8 cut(s) 21, 309, 574, 794, 1498, 1903, 2080, 2848
MunI CAATTG 1 cut(s) 2412
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 7 cut(s) 309, 574, 794, 1498, 1903, 2080, 2848
MvnI CGCG 1 cut(s) 2408
MwoI GCNNNNNNNGC 9 cut(s) 395, 761, 1280, 2345, 2414, 2428, 2441, 2471, 2690
NciI CCSGG 1 cut(s) 21
NdeI CATATG 3 cut(s) 183, 424, 2205
NdeII GATC 4 cut(s) 951, 1169, 2098, 2208
NlaIV GGNNCC 5 cut(s) 18, 24, 557, 1891, 2845
NmeAIII GCCGAG 2 cut(s) 2420, 2485
NmuCI GTSAC 2 cut(s) 545, 1639
NsbI TGCGCA 1 cut(s) 2436
NsiI ATGCAT 6 cut(s) 6, 189, 811, 1260, 1774, 2205
NspI RCATGY 3 cut(s) 1258, 1950, 2435
NspV TTCGAA 2 cut(s) 1107, 2635
PaeI GCATGC 1 cut(s) 2435
PceI AGGCCT 4 cut(s) 199, 632, 2078, 2422
PciI ACATGT 1 cut(s) 1946
PctI GAATGC 1 cut(s) 299
PfeI GAWTC 3 cut(s) 734, 787, 1189
PflFI GACNNNGTC 1 cut(s) 431
PfoI TCCNGGA 1 cut(s) 792
PleI GAGTC 5 cut(s) 440, 1454, 2047, 2522, 2725
PpsI GAGTC 5 cut(s) 440, 1454, 2047, 2522, 2725
Ppu21I YACGTR 1 cut(s) 1922
PpuMI RGGWCCY 1 cut(s) 241
PscI ACATGT 1 cut(s) 1946
PshBI ATTAAT 5 cut(s) 317, 1145, 1322, 2325, 2448
Psp124BI GAGCTC 1 cut(s) 1006
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 7 cut(s) 307, 572, 792, 1496, 1901, 2078, 2846
PspEI GGTNACC 1 cut(s) 545
PspFI CCCAGC 1 cut(s) 2867
PspGI CCWGG 7 cut(s) 307, 572, 792, 1496, 1901, 2078, 2846
PspN4I GGNNCC 5 cut(s) 18, 24, 557, 1891, 2845
PspOMI GGGCCC 1 cut(s) 22
PspPI GGNCC 4 cut(s) 22, 23, 241, 556
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 2098
PsyI GACNNNGTC 1 cut(s) 431
RsaI GTAC 7 cut(s) 306, 517, 1009, 1474, 1549, 1721, 2754
RsaNI GTAC 7 cut(s) 305, 516, 1008, 1473, 1548, 1720, 2753
RseI CAYNNNNRTG 4 cut(s) 1402, 1704, 2011, 2204
SacI GAGCTC 1 cut(s) 1006
Sau3AI GATC 4 cut(s) 951, 1169, 2098, 2208
Sau96I GGNCC 4 cut(s) 22, 23, 241, 556
ScaI AGTACT 1 cut(s) 1474
SchI GAGTC 5 cut(s) 441, 1455, 2047, 2522, 2725
ScrFI CCNGG 8 cut(s) 21, 309, 574, 794, 1498, 1903, 2080, 2848
SduI GDGCHC 5 cut(s) 26, 222, 703, 1006, 1894
SexAI ACCWGGT 1 cut(s) 1901
SfuI TTCGAA 2 cut(s) 1107, 2635
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 4 cut(s) 1402, 1704, 2011, 2204
SmlI CTYRAG 2 cut(s) 1663, 1759
SmoI CTYRAG 2 cut(s) 1663, 1759
SphI GCATGC 1 cut(s) 2435
SseBI AGGCCT 4 cut(s) 199, 632, 2078, 2422
SsiI CCGC 4 cut(s) 263, 1376, 1421, 2408
SspI AATATT 2 cut(s) 1349, 2827
SspMI CTAG 7 cut(s) 245, 552, 1271, 1512, 1788, 2694, 2872
SstI GAGCTC 1 cut(s) 1006
StuI AGGCCT 4 cut(s) 199, 632, 2078, 2422
StyD4I CCNGG 8 cut(s) 19, 307, 572, 792, 1496, 1901, 2078, 2846
StyI CCWWGG 3 cut(s) 551, 934, 2497
TaaI ACNGT 7 cut(s) 145, 254, 520, 680, 875, 1527, 2654
TaiI ACGT 3 cut(s) 219, 1441, 1924
TaqI TCGA 6 cut(s) 236, 435, 638, 1107, 2587, 2635
TaqII GACCGA 3 cut(s) 1027, 1701, 1991
TatI WGTACW 3 cut(s) 1472, 1719, 2752
TauI GCSGC 2 cut(s) 265, 2411
TfiI GAWTC 3 cut(s) 734, 787, 1189
TscAI CASTG 2 cut(s) 2123, 2219
TseFI GTSAC 2 cut(s) 545, 1639
TseI GCWGC 8 cut(s) 761, 840, 1373, 1652, 1679, 1959, 2063, 2546
Tsp45I GTSAC 2 cut(s) 545, 1639
TspDTI ATGAA 9 cut(s) 153, 523, 816, 1005, 1181, 1308, 1357, 1397, 2403
TspGWI ACGGA 3 cut(s) 1439, 1643, 2522
TspRI CASTG 2 cut(s) 2123, 2219
Tth111I GACNNNGTC 1 cut(s) 431
VneI GTGCAC 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 241
VspI ATTAAT 5 cut(s) 317, 1145, 1322, 2325, 2448
XagI CCTNNNNNAGG 2 cut(s) 637, 2307
XapI RAATTY 3 cut(s) 1315, 2023, 2637
XceI RCATGY 3 cut(s) 1258, 1950, 2435
XmaJI CCTAGG 1 cut(s) 551
XmiI GTMKAC 3 cut(s) 168, 580, 2491
XspI CTAG 7 cut(s) 245, 552, 1271, 1512, 1788, 2694, 2872
ZrmI AGTACT 1 cut(s) 1474
Zsp2I ATGCAT 6 cut(s) 6, 189, 811, 1260, 1774, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.