pycom17g23230

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000671
Physical Location & Seq
Forward (+)
21641321 .. 21642292
972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGTCTGTAGCCAAGAGGGTGGCTGAAGAGTTTGGTTGTCGTTTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACCGATGGTATGCTTCTTAGGGAGATTTTGATTGATGAGAACCTTTCCCAATACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCACACAGATGTTCTTTTTGGATTACTGAAGAAGCTTCTGATCCGGAGACCAGATCTTCGTTTGATTGTCACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATATTTACTATCCCCGGGAGAACGTTTCCTGTCGAGATACTCTACACCAAGCAGCCAGAAAGTGACTACCTTGATGCATCTCTAATTACTGTCCTACAAATCCACTTAACAGAACCTGAAGGTGACATCCTTCTTTTCTTGACTGGACAAGAAGAGATTGATTTTGCATGCCAGTCTCTTTATGAGAGGATGAAGGGTCTTGGTAAAAATGTTCCTGAGTTAATTATTCTACCAGTATATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCGATCTGCCCCGCCAGGGAAGAGGAAAGTGGTTGTGGCTACTAATATTGCTGAAGCATCACTGACTATTGATGGGATATTTTATGTCATCGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTGGATTCGCTTGTCATTACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGGCGTGCTGGACGCACAGGGCCTGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACCGTAATGAGATGTCCCCTACTTCAATTCCAGAAATCCAGAGGATAAATCTTGGGATGACTACACTTACGATGAAAGCCATGGGGATAAATGATCGATCCTGTCTTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

324

Amino Acids

36.13

Weight (kDa)

5.33

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 142 - 273 2.2e-11 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 2 cut(s) 620, 835
AclI AACGTT 1 cut(s) 353
AclWI GGATC 3 cut(s) 241, 695, 948
AcuI CTGAAG 4 cut(s) 45, 254, 468, 681
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 624, 739, 740
AgsI TTSAA 1 cut(s) 882
AjnI CCWGG 3 cut(s) 622, 703, 820
AluBI AGCT 3 cut(s) 191, 241, 797
AluI AGCT 3 cut(s) 191, 241, 797
Alw26I GTCTC 2 cut(s) 247, 510
AlwI GGATC 3 cut(s) 241, 695, 948
AlwNI CAGNNNCTG 4 cut(s) 98, 446, 745, 821
Ama87I CYCGRG 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 1 cut(s) 818
ApeKI GCWGC 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
AspS9I GGNCC 2 cut(s) 89, 818
AsuC2I CCSGG 2 cut(s) 345, 346
AsuHPI GGTGA 2 cut(s) 268, 464
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 577
BbvI GCAGC 1 cut(s) 394
BccI CCATC 2 cut(s) 113, 674
BciT130I CCWGG 3 cut(s) 624, 705, 822
BcnI CCSGG 2 cut(s) 345, 346
BcoDI GTCTC 2 cut(s) 247, 510
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 259
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 5 cut(s) 345, 346, 624, 705, 822
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 89, 818
BmrFI CCNGG 5 cut(s) 345, 346, 624, 705, 822
BmsI GCATC 5 cut(s) 283, 394, 416, 674, 788
BpuMI CCSGG 2 cut(s) 345, 346
Bsa29I ATCGAT 3 cut(s) 610, 699, 952
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 5 cut(s) 343, 344, 623, 821, 936
BsaWI WCCGGW 1 cut(s) 249
Bsc4I CCNNNNNNNGG 3 cut(s) 624, 739, 740
Bse1I ACTGG 4 cut(s) 91, 478, 502, 563
BseAI TCCGGA 1 cut(s) 249
BseBI CCWGG 3 cut(s) 624, 705, 822
BseCI ATCGAT 3 cut(s) 610, 699, 952
BseDI CCNNGG 5 cut(s) 343, 344, 623, 821, 936
BseGI GGATG 4 cut(s) 298, 456, 525, 918
BseLI CCNNNNNNNGG 3 cut(s) 624, 739, 740
BseMII CTCAG 2 cut(s) 537, 837
BseNI ACTGG 4 cut(s) 91, 478, 502, 563
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 797
BshFI GGCC 1 cut(s) 820
BshVI ATCGAT 3 cut(s) 610, 699, 952
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 2 cut(s) 250, 345
BslFI GGGAC 1 cut(s) 856
BslI CCNNNNNNNGG 3 cut(s) 624, 739, 740
BsmAI GTCTC 2 cut(s) 247, 510
BsmFI GGGAC 1 cut(s) 856
BsnI GGCC 1 cut(s) 820
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 577
Bsp13I TCCGGA 1 cut(s) 249
Bsp143I GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 620, 835
BspANI GGCC 1 cut(s) 820
BspCNI CTCAG 2 cut(s) 538, 838
BspDI ATCGAT 3 cut(s) 610, 699, 952
BspEI TCCGGA 1 cut(s) 249
BspHI TCATGA 1 cut(s) 193
BspPI GGATC 3 cut(s) 241, 695, 948
BspTNI GGTCTC 1 cut(s) 247
BsrI ACTGG 4 cut(s) 91, 478, 502, 563
BssECI CCNNGG 5 cut(s) 343, 344, 623, 821, 936
BssMI GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
BssT1I CCWWGG 1 cut(s) 936
Bst2UI CCWGG 3 cut(s) 624, 705, 822
Bst4CI ACNGT 4 cut(s) 100, 326, 421, 860
Bst6I CTCTTC 3 cut(s) 21, 477, 623
BstC8I GCNNGC 2 cut(s) 499, 804
BstDEI CTNAG 3 cut(s) 131, 546, 846
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 4 cut(s) 298, 456, 525, 918
BstKTI GATC 7 cut(s) 249, 262, 610, 614, 703, 952, 956
BstMAI GTCTC 2 cut(s) 247, 510
BstMBI GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
BstMWI GCNNNNNNNGC 2 cut(s) 751, 803
BstNI CCWGG 3 cut(s) 624, 705, 822
BstNSI RCATGY 1 cut(s) 501
BstSCI CCNGG 5 cut(s) 343, 344, 622, 703, 820
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 259
Bsu15I ATCGAT 3 cut(s) 610, 699, 952
BsuRI GGCC 1 cut(s) 820
BsuTUI ATCGAT 3 cut(s) 610, 699, 952
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 4 cut(s) 298, 456, 525, 918
BtsIMutI CAGTG 3 cut(s) 84, 668, 843
Cac8I GCNNGC 2 cut(s) 499, 804
CaiI CAGNNNCTG 4 cut(s) 98, 446, 745, 821
CciI TCATGA 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 89, 818
Cfr9I CCCGGG 1 cut(s) 344
ClaI ATCGAT 3 cut(s) 610, 699, 952
CseI GACGC 1 cut(s) 819
Csp6I GTAC 1 cut(s) 109
CviAII CATG 5 cut(s) 112, 178, 194, 498, 937
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 3 cut(s) 131, 546, 846
DpnI GATC 7 cut(s) 248, 261, 609, 613, 702, 951, 955
DpnII GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
Eam1104I CTCTTC 3 cut(s) 21, 477, 623
EarI CTCTTC 3 cut(s) 21, 477, 623
Eco130I CCWWGG 1 cut(s) 936
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 4 cut(s) 45, 254, 468, 681
Eco88I CYCGRG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 818
EcoRII CCWGG 3 cut(s) 622, 703, 820
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 5 cut(s) 115, 181, 197, 501, 940
FaqI GGGAC 1 cut(s) 856
FatI CATG 5 cut(s) 111, 177, 193, 497, 936
FauI CCCGC 1 cut(s) 627
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 4 cut(s) 305, 443, 532, 925
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 582
GluI GCNGC 1 cut(s) 383
GsaI CCCAGC 1 cut(s) 801
HaeIII GGCC 1 cut(s) 820
HapII CCGG 2 cut(s) 250, 345
HgaI GACGC 1 cut(s) 819
Hin1II CATG 5 cut(s) 115, 181, 197, 501, 940
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 1 cut(s) 749
HpaII CCGG 2 cut(s) 250, 345
HphI GGTGA 2 cut(s) 268, 464
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 7 cut(s) 194, 250, 364, 469, 545, 887, 895
HpyAV CCTTC 4 cut(s) 443, 470, 517, 587
HpyCH4III ACNGT 4 cut(s) 100, 326, 421, 860
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 7 cut(s) 84, 296, 407, 497, 592, 713, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 751, 803
HpyF3I CTNAG 3 cut(s) 131, 546, 846
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 5 cut(s) 115, 181, 197, 501, 940
Kpn2I TCCGGA 1 cut(s) 249
Kzo9I GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 5 cut(s) 283, 394, 416, 674, 788
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 5 cut(s) 274, 326, 392, 452, 830
MalI GATC 7 cut(s) 248, 261, 609, 613, 702, 951, 955
MboI GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
MboII GAAGA 6 cut(s) 38, 247, 254, 310, 494, 640
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 4 cut(s) 414, 552, 768, 882
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 9 cut(s) 9, 43, 70, 192, 289, 510, 624, 848, 891
Mph1103I ATGCAT 1 cut(s) 409
MroI TCCGGA 1 cut(s) 249
MseI TTAA 2 cut(s) 437, 551
MslI CAYNNNNRTG 1 cut(s) 213
MspI CCGG 2 cut(s) 250, 345
MspR9I CCNGG 5 cut(s) 345, 346, 624, 705, 822
MvaI CCWGG 3 cut(s) 624, 705, 822
MwoI GCNNNNNNNGC 2 cut(s) 751, 803
NciI CCSGG 2 cut(s) 345, 346
NcoI CCATGG 1 cut(s) 936
NdeII GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
NlaIII CATG 5 cut(s) 115, 181, 197, 501, 940
NmuCI GTSAC 3 cut(s) 274, 392, 452
NsiI ATGCAT 1 cut(s) 409
NspI RCATGY 1 cut(s) 501
PaeI GCATGC 1 cut(s) 501
PagI TCATGA 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 799
PfeI GAWTC 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 1 cut(s) 384
PsiI TTATAA 1 cut(s) 729
Psp1406I AACGTT 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 622, 703, 820
PspFI CCCAGC 1 cut(s) 797
PspGI CCWGG 3 cut(s) 622, 703, 820
PspPI GGNCC 2 cut(s) 89, 818
PstNI CAGNNNCTG 4 cut(s) 98, 446, 745, 821
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 437, 551
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 7 cut(s) 246, 259, 607, 611, 700, 949, 953
Sau96I GGNCC 2 cut(s) 89, 818
ScrFI CCNGG 5 cut(s) 345, 346, 624, 705, 822
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 5 cut(s) 283, 394, 416, 674, 788
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 89
SmaI CCCGGG 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 213
SphI GCATGC 1 cut(s) 501
Sse9I AATT 4 cut(s) 414, 552, 768, 882
SsiI CCGC 2 cut(s) 620, 835
SspI AATATT 1 cut(s) 655
SspMI CTAG 1 cut(s) 582
StyD4I CCNGG 5 cut(s) 343, 344, 622, 703, 820
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 4 cut(s) 100, 326, 421, 860
TaiI ACGT 1 cut(s) 356
TaqI TCGA 5 cut(s) 321, 363, 610, 699, 952
TaqII GACCGA 1 cut(s) 131
TasI AATT 4 cut(s) 414, 552, 768, 882
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 1 cut(s) 749
Tru1I TTAA 2 cut(s) 437, 551
Tru9I TTAA 2 cut(s) 437, 551
TscAI CASTG 3 cut(s) 91, 675, 850
TseFI GTSAC 3 cut(s) 274, 392, 452
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 3 cut(s) 274, 392, 452
TspDTI ATGAA 3 cut(s) 201, 536, 944
TspMI CCCGGG 1 cut(s) 344
TspRI CASTG 3 cut(s) 91, 675, 850
VpaK11BI GGWCC 1 cut(s) 89
XceI RCATGY 1 cut(s) 501
XmaI CCCGGG 1 cut(s) 344
XspI CTAG 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.