pycom17g23240

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000671
Physical Location & Seq
Forward (+)
21642394 .. 21643811
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGGCTGAGTTTCCTCTCGATCCACCGTTATCTAAGATGCTACTCGCTAGTGTAGACCTTGGATGTAGTGATGAGATCTTGACCATCATTGCAATGATTCAGACAGGCAATATCTTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCTAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAATTTCTCAGGTCCATGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCTTGAGGAGGGCACAGGATGTTAGAAAACAACTTCTGAGCATCATGGACAAGTATAAATTGGACGTGGTCAGTGCAGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACAGCAGGTTTCTTCTTCCATGGTGCTAGAAAGGACCCACAGGAGGGTTATAGAACCCTAGTCGAGAACCAGCCAGTTTATATCCACCCAAGCAGTGCTCTCTTCCAGAGACAACCAGATTGGGTCATCTACCATGAGCTGGTTATGACTACAAAGGAGTACATGCGCGAGGTGACAGTCGTTGACCCCAAATGGCTAGTAGAACTGGCACCAAGGTTCTTCAAAGTATCAGATCCCACAAAGATGAGTAAGCGCAAGCGTCAGGAACGAATTGAACCCTTATATGACAGATACCATGAACCGAACTCTTGGCGTCTTAGTAAGCGTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

240

Amino Acids

28.11

Weight (kDa)

9.67

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 7 - 66 6e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 124 - 200 6.2e-27 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 592
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 552
AclWI GGATC 3 cut(s) 14, 267, 611
AcsI RAATTY 2 cut(s) 229, 358
AcyI GRCGYC 1 cut(s) 697
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 5 cut(s) 178, 282, 427, 428, 523
AgsI TTSAA 2 cut(s) 607, 659
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 3 cut(s) 161, 224, 523
AluI AGCT 3 cut(s) 161, 224, 523
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 3 cut(s) 177, 273, 487
AlwI GGATC 3 cut(s) 14, 267, 611
AoxI GGCC 1 cut(s) 122
ApoI RAATTY 2 cut(s) 229, 358
AspA2I CCTAGG 1 cut(s) 125
AspLEI GCGC 2 cut(s) 552, 639
AspS9I GGNCC 2 cut(s) 239, 418
AsuHPI GGTGA 1 cut(s) 568
AvaII GGWCC 2 cut(s) 239, 418
AvrII CCTAGG 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 289
BanI GGYRCC 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 484
BccI CCATC 2 cut(s) 92, 195
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 3 cut(s) 177, 273, 487
BfaI CTAG 5 cut(s) 48, 126, 411, 443, 581
BfuAI ACCTGC 1 cut(s) 380
BglII AGATCT 1 cut(s) 75
BlnI CCTAGG 1 cut(s) 125
Bme1390I CCNGG 1 cut(s) 143
Bme18I GGWCC 2 cut(s) 239, 418
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 239, 418
BmiI GGNNCC 2 cut(s) 420, 594
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 27, 324
BpuEI CTTGAG 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 697
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 6 cut(s) 58, 125, 141, 242, 403, 596
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 282, 427, 428, 523
Bse1I ACTGG 2 cut(s) 458, 594
Bse3DI GCAATG 2 cut(s) 87, 99
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 6 cut(s) 58, 125, 141, 242, 403, 596
BseGI GGATG 2 cut(s) 68, 298
BseLI CCNNNNNNNGG 5 cut(s) 178, 282, 427, 428, 523
BseMI GCAATG 2 cut(s) 87, 99
BseMII CTCAG 2 cut(s) 249, 302
BseNI ACTGG 2 cut(s) 458, 594
BseRI GAGGAG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 289
BsgI GTGCAG 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 484
BslI CCNNNNNNNGG 5 cut(s) 178, 282, 427, 428, 523
BsmAI GTCTC 3 cut(s) 177, 273, 487
BsnI GGCC 1 cut(s) 124
Bso31I GGTCTC 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 289, 484
Bsp143I GATC 6 cut(s) 19, 75, 148, 272, 369, 616
Bsp19I CCATGG 2 cut(s) 242, 403
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 2 cut(s) 248, 303
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 420, 594
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 14, 267, 611
BspT107I GGYRCC 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 2 cut(s) 87, 99
BsrI ACTGG 2 cut(s) 458, 594
BssECI CCNNGG 6 cut(s) 58, 125, 141, 242, 403, 596
BssMI GATC 6 cut(s) 19, 75, 148, 272, 369, 616
BssNI GRCGYC 1 cut(s) 697
BssT1I CCWWGG 5 cut(s) 58, 125, 242, 403, 596
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 27, 562
Bst6I CTCTTC 2 cut(s) 149, 491
BstACI GRCGYC 1 cut(s) 697
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 7 cut(s) 6, 33, 162, 225, 235, 311, 701
BstDSI CCRYGG 2 cut(s) 242, 403
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 68, 298
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 2 cut(s) 552, 639
BstKTI GATC 6 cut(s) 22, 78, 151, 275, 372, 619
BstMAI GTCTC 3 cut(s) 177, 273, 487
BstMBI GATC 6 cut(s) 19, 75, 148, 272, 369, 616
BstMWI GCNNNNNNNGC 2 cut(s) 221, 386
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 141
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 1 cut(s) 552
BstX2I RGATCY 2 cut(s) 75, 616
BstYI RGATCY 2 cut(s) 75, 616
BsuRI GGCC 1 cut(s) 124
BtgI CCRYGG 2 cut(s) 242, 403
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 68, 298
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 2 cut(s) 352, 484
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 2 cut(s) 552, 639
Cfr13I GGNCC 2 cut(s) 239, 418
CseI GACGC 3 cut(s) 632, 686, 698
Csp6I GTAC 1 cut(s) 544
CviAII CATG 6 cut(s) 243, 319, 404, 518, 547, 680
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 7 cut(s) 6, 33, 162, 225, 235, 311, 701
DpnI GATC 6 cut(s) 21, 77, 150, 274, 371, 618
DpnII GATC 6 cut(s) 19, 75, 148, 272, 369, 616
Eam1104I CTCTTC 2 cut(s) 149, 491
EarI CTCTTC 2 cut(s) 149, 491
Eco130I CCWWGG 5 cut(s) 58, 125, 242, 403, 596
Eco147I AGGCCT 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 239, 418
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 5 cut(s) 58, 125, 242, 403, 596
ErhI CCWWGG 5 cut(s) 58, 125, 242, 403, 596
FaeI CATG 6 cut(s) 246, 322, 407, 521, 550, 683
FatI CATG 6 cut(s) 242, 318, 403, 517, 546, 679
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 54
FokI GGATG 2 cut(s) 75, 305
FspBI CTAG 5 cut(s) 48, 126, 411, 443, 581
GlaI GCGC 2 cut(s) 551, 638
HaeIII GGCC 1 cut(s) 124
HgaI GACGC 3 cut(s) 632, 686, 698
HhaI GCGC 2 cut(s) 552, 639
Hin1I GRCGYC 1 cut(s) 697
Hin1II CATG 6 cut(s) 246, 322, 407, 521, 550, 683
Hin6I GCGC 2 cut(s) 550, 637
HinP1I GCGC 2 cut(s) 550, 637
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 1 cut(s) 97
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 2 cut(s) 55, 568
Hpy188I TCNGA 5 cut(s) 102, 153, 192, 312, 616
Hpy188III TCNNGA 7 cut(s) 17, 79, 270, 373, 448, 490, 647
Hpy8I GTNNAC 2 cut(s) 55, 568
Hpy99I CGWCG 1 cut(s) 714
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 27, 562
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 92, 203, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 386
HpyF3I CTNAG 7 cut(s) 6, 33, 162, 225, 235, 311, 701
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 697
Hsp92II CATG 6 cut(s) 246, 322, 407, 521, 550, 683
HspAI GCGC 2 cut(s) 550, 637
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 6 cut(s) 19, 75, 148, 272, 369, 616
LweI GCATC 2 cut(s) 27, 324
MaeI CTAG 5 cut(s) 48, 126, 411, 443, 581
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 556
MalI GATC 6 cut(s) 21, 77, 150, 274, 371, 618
MboI GATC 6 cut(s) 19, 75, 148, 272, 369, 616
MboII GAAGA 5 cut(s) 166, 388, 391, 478, 595
MflI RGATCY 2 cut(s) 75, 616
MhlI GDGCHC 2 cut(s) 289, 484
MluCI AATT 5 cut(s) 229, 332, 358, 381, 654
MnlI CCTC 7 cut(s) 24, 172, 205, 273, 276, 421, 547
MslI CAYNNNNRTG 2 cut(s) 92, 626
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 221, 386
NcoI CCATGG 2 cut(s) 242, 403
NdeII GATC 6 cut(s) 19, 75, 148, 272, 369, 616
NlaIII CATG 6 cut(s) 246, 322, 407, 521, 550, 683
NlaIV GGNNCC 2 cut(s) 420, 594
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 550
PceI AGGCCT 1 cut(s) 124
PfeI GAWTC 1 cut(s) 97
PflFI GACNNNGTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 523
PpuMI RGGWCCY 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 420, 594
PspPI GGNCC 2 cut(s) 239, 418
PspPPI RGGWCCY 1 cut(s) 418
PsuI RGATCY 2 cut(s) 75, 616
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
RseI CAYNNNNRTG 2 cut(s) 92, 626
Sau3AI GATC 6 cut(s) 19, 75, 148, 272, 369, 616
Sau96I GGNCC 2 cut(s) 239, 418
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 2 cut(s) 289, 484
SetI ASST 9 cut(s) 60, 163, 226, 241, 342, 394, 525, 558, 602
SfaNI GCATC 2 cut(s) 27, 324
SinI GGWCC 2 cut(s) 239, 418
SmiMI CAYNNNNRTG 2 cut(s) 92, 626
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
Sse9I AATT 5 cut(s) 229, 332, 358, 381, 654
SseBI AGGCCT 1 cut(s) 124
SspMI CTAG 5 cut(s) 48, 126, 411, 443, 581
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 5 cut(s) 58, 125, 242, 403, 596
TaaI ACNGT 2 cut(s) 27, 562
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 18, 271, 447
TasI AATT 5 cut(s) 229, 332, 358, 381, 654
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 1 cut(s) 97
TscAI CASTG 2 cut(s) 352, 484
TseFI GTSAC 1 cut(s) 556
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 1 cut(s) 696
TspRI CASTG 2 cut(s) 352, 484
Tth111I GACNNNGTC 1 cut(s) 341
Van91I CCANNNNNTGG 1 cut(s) 523
VpaK11BI GGWCC 2 cut(s) 239, 418
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 2 cut(s) 229, 358
XceI RCATGY 1 cut(s) 550
XmaJI CCTAGG 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 5 cut(s) 48, 126, 411, 443, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.