RchiOBHm_Chr7g0182091

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001470
Physical Location & Seq
Reverse (-)
3475490 .. 3477450
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16242

Sequence Viewer

Length: 1821 bp
ATGGCAAGCAGAGGCAAACGATCTCTTTTCAAATCTGCCAGAGATAGGATATGGCACAAGGTGAATGGTTGGAAAGAAAAAAAGTTGTCACAGGCAGGCAAAATGATCCTTTTGAAATCGGTTGTTCAAGCAATACCCTCATACTCGATGAGTGTCTTTAGATTGCCATTGTATTTATGTCATGATCTATCGGGATTGTCATCGCGGTTTTGGTGGGGACGAAAGAGGGGAAAAAAGGGTGTGCATTGGTTGAAGTGGAGTTCTCTTTGCCAAAATAAAAAGAATGGAGGTCTGGGTTTTAGAGACTTTGTGGAGTTTAATCAGGCTTTGCTTGCCAAGCAGTGTTGGAGATTGTTTGCTTCGCCGGATTTACTGGTTTCTCAGATTTACAAGTGCAAATATTACCCTGAATCTTCTTTCTTAGAAGCAAGGGTTGGGAGATCTAGCTCATTGATATGGAGATCATTACTGTGGGGGAGAAAATTGTTGCTTTCTGGTTTGAGATGGAGGGTTGGGGATGGTACATCTATTCTGATCTACCAGGATGCTTGGGTGCCAATCCCTTACTCTTTCAAAATTATGTCTCCTCCAAATCTGCTGGTTAATCGCCCTGTCTCTTATTTAATTTCAAATGGTTCCTGGAACTTTGAACTTATTTCAGGATCTTTTTGGGAGTCTGAAGTGTCAGCTATCTGTTCTATTCCTTTGCCTATTACTCCTTGTTCAGATAAACAAGTTTGGCACTATACAAAAAATGGAACTTATACTGTGAAGAGTGGTTATCAGCTTGCTCAGACTTTGAGGATTCGAGGTGCCGGACAAGAGGGAAGCTCTACTAATCAGTTGGACTTGGTTTGGCAGGTTATGTGGAAATTGAAAATTCCTGAGGGAGTTAAAGTTTTTCTTTGGAGGGTGATTCATGAGATTTTGCCAACAGCTTTGTTGTTACAACGTAGACATCTCCTTGCTTCTTCAGTTTGCTCTAGATGCCAAGGTGAGGAGGAGACTATTCTACATGCCTTTTGGTCTTGTTCAAAGGCTAGGGAGGTCTGGAAGCTTACTCCTTTCAAGAGTGCAGTGGGTAGAGGACATTATGAGAATTTCAAAGATATGTTCAGATTTGCAACATCAGTTCTTACTTTGAATGAATTGGGTTTGGTGATTCTCATTTGTAGAAGGATTTGGCAGGCTCGTAATGATGCAATTTTCAACCACAAACTAGTCAGTCCTCAATTTCTTTATGCTCAAGCTCTTCAATTGCAGCAAGATTTTCTGGAGAAACAATTACAGTCTAGCTACCATGCTAAGAGGCCTACTGCTCAGGTTAGATGGACACGACCTTCTTTTCCTTTTCTGAAACTGAATGTGGATGGTGCTGTAAATGTTGTTCTTGGTTTAAGAGGACTAGGAGCAGTCCTCCGGAATGAAAATGGTGATTTATTGATTGCGGTTTCAAAAGGAATGACTGGTGGTTTTTCTGTCAAAGCTACTGAACTATATGCTGCAGTCTTGGGTTTTCAGACTATTATCCAAGCAGGTTTTCAATCGACACAAATCATTTTGGAAATGGATGCTTTGGGAGTAATTAGTGATCTGAATGCAGCAGATATCAATTGGTCGATTGAAGGTGCTTTGGTTGAAGAAGTGCGAAATCTTTTCCGCTTTTTCTCTTCAGTTATATGTACATATAGCCCTAGGAAATGTAATCAGGCAGCGCATACTTTAGCAAAAAATGCGCTTTTGTTTCCTGCATTTCAAGTTTGGGTTGAGGAGGGACCACAATGGCTCTCCCGTGTACTTATTTCTGATGTAACTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

606

Amino Acids

68.91

Weight (kDa)

9.83

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 281 - 351 1.2e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 455 - 579 1.8e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 850, 1526
AccB1I GGYRCC 2 cut(s) 553, 812
AccI GTMKAC 1 cut(s) 955
AccII CGCG 1 cut(s) 205
AccIII TCCGGA 1 cut(s) 1419
AciI CCGC 3 cut(s) 205, 1448, 1660
AclWI GGATC 2 cut(s) 100, 670
AcsI RAATTY 2 cut(s) 879, 1099
AcuI CTGAAG 3 cut(s) 699, 957, 1656
AdeI CACNNNGTG 1 cut(s) 61
AfaI GTAC 3 cut(s) 523, 1684, 1797
AfiI CCNNNNNNNGG 2 cut(s) 45, 997
AhlI ACTAGT 1 cut(s) 1219
AjnI CCWGG 2 cut(s) 540, 638
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 1524, 1556
AluBI AGCT 9 cut(s) 447, 689, 787, 831, 938, 1057, 1250, 1296, 1487
AluI AGCT 9 cut(s) 447, 689, 787, 831, 938, 1057, 1250, 1296, 1487
Alw26I GTCTC 4 cut(s) 297, 588, 619, 998
AlwI GGATC 2 cut(s) 100, 670
Aor13HI TCCGGA 1 cut(s) 1419
AoxI GGCC 1 cut(s) 1310
ApeKI GCWGC 4 cut(s) 1261, 1502, 1601, 1712
ApoI RAATTY 2 cut(s) 879, 1099
AspA2I CCTAGG 1 cut(s) 1694
AspLEI GCGC 2 cut(s) 1717, 1738
AspS9I GGNCC 1 cut(s) 1775
AsuHPI GGTGA 5 cut(s) 73, 925, 1007, 1171, 1445
AvaII GGWCC 1 cut(s) 1775
AvrII CCTAGG 1 cut(s) 1694
AxyI CCTNAGG 1 cut(s) 885
BanI GGYRCC 2 cut(s) 553, 812
BbvI GCAGC 4 cut(s) 1273, 1489, 1613, 1724
BccI CCATC 4 cut(s) 498, 512, 1323, 1364
BciT130I CCWGG 2 cut(s) 542, 640
BcoDI GTCTC 4 cut(s) 297, 588, 619, 998
BcuI ACTAGT 1 cut(s) 1219
BfaI CTAG 8 cut(s) 444, 984, 1041, 1220, 1293, 1406, 1695, 1819
BfmI CTRYAG 1 cut(s) 1503
BfuAI ACCTGC 2 cut(s) 850, 1526
BglII AGATCT 1 cut(s) 440
BisI GCNGC 4 cut(s) 1262, 1503, 1602, 1713
BlnI CCTAGG 1 cut(s) 1694
BlsI GCNGC 4 cut(s) 1263, 1504, 1603, 1714
Bme1390I CCNGG 2 cut(s) 542, 640
Bme18I GGWCC 1 cut(s) 1775
BmgT120I GGNCC 1 cut(s) 1775
BmiI GGNNCC 4 cut(s) 555, 637, 814, 1776
BmrFI CCNGG 2 cut(s) 542, 640
BmsI GCATC 4 cut(s) 535, 977, 1189, 1561
BplI GAGNNNNNCTC 4 cut(s) 815, 847, 1401, 1433
BpmI CTGGAG 1 cut(s) 1295
Bpu10I CCTNAGC 1 cut(s) 1320
BpuEI CTTGAG 1 cut(s) 1230
BsaBI GATNNNNATC 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 991, 1694
BsaWI WCCGGW 1 cut(s) 1419
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 997
Bse1I ACTGG 2 cut(s) 378, 1471
Bse21I CCTNAGG 1 cut(s) 885
Bse8I GATNNNNATC 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 1419
BseBI CCWGG 2 cut(s) 542, 640
BseDI CCNNGG 2 cut(s) 991, 1694
BseGI GGATG 4 cut(s) 523, 550, 1375, 1576
BseJI GATNNNNATC 1 cut(s) 199
BseLI CCNNNNNNNGG 2 cut(s) 45, 997
BseMII CTCAG 4 cut(s) 395, 806, 876, 1334
BseNI ACTGG 2 cut(s) 378, 1471
BseRI GAGGAG 4 cut(s) 576, 1013, 1016, 1784
BseXI GCAGC 4 cut(s) 1273, 1489, 1613, 1724
BsgI GTGCAG 1 cut(s) 1095
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 1312
BshNI GGYRCC 2 cut(s) 553, 812
BsiSI CCGG 3 cut(s) 365, 816, 1420
BslFI GGGAC 2 cut(s) 231, 1788
BslI CCNNNNNNNGG 2 cut(s) 45, 997
BsmAI GTCTC 4 cut(s) 297, 588, 619, 998
BsmFI GGGAC 2 cut(s) 231, 1788
BsmI GAATGC 1 cut(s) 1603
BsnI GGCC 1 cut(s) 1312
Bsp13I TCCGGA 1 cut(s) 1419
Bsp1407I TGTACA 1 cut(s) 1682
Bsp143I GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
BspACI CCGC 3 cut(s) 205, 1448, 1660
BspANI GGCC 1 cut(s) 1312
BspCNI CTCAG 4 cut(s) 394, 805, 877, 1333
BspEI TCCGGA 1 cut(s) 1419
BspFNI CGCG 1 cut(s) 205
BspHI TCATGA 2 cut(s) 181, 919
BspLI GGNNCC 4 cut(s) 555, 637, 814, 1776
BspMAI CTGCAG 1 cut(s) 1507
BspMI ACCTGC 2 cut(s) 850, 1526
BspPI GGATC 2 cut(s) 100, 670
BspQI GCTCTTC 1 cut(s) 1257
BspT107I GGYRCC 2 cut(s) 553, 812
BsrGI TGTACA 1 cut(s) 1682
BsrI ACTGG 2 cut(s) 378, 1471
BssECI CCNNGG 2 cut(s) 991, 1694
BssMI GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
BssT1I CCWWGG 2 cut(s) 991, 1694
Bst2UI CCWGG 2 cut(s) 542, 640
Bst4CI ACNGT 3 cut(s) 471, 769, 1290
Bst6I CTCTTC 3 cut(s) 767, 1257, 1675
BstAPI GCANNNNNTGC 1 cut(s) 1733
BstAUI TGTACA 1 cut(s) 1682
BstC8I GCNNGC 5 cut(s) 7, 97, 333, 789, 1188
BstDEI CTNAG 6 cut(s) 381, 421, 792, 885, 1305, 1320
BstF5I GGATG 4 cut(s) 523, 550, 1375, 1576
BstFNI CGCG 1 cut(s) 205
BstHHI GCGC 2 cut(s) 1717, 1738
BstKTI GATC 8 cut(s) 23, 108, 187, 443, 464, 537, 665, 1594
BstMAI GTCTC 4 cut(s) 297, 588, 619, 998
BstMBI GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
BstMWI GCNNNNNNNGC 4 cut(s) 332, 337, 987, 1733
BstNI CCWGG 2 cut(s) 542, 640
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 2 cut(s) 540, 638
BstSFI CTRYAG 1 cut(s) 1503
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 4 cut(s) 1273, 1489, 1613, 1724
BstX2I RGATCY 2 cut(s) 440, 662
BstYI RGATCY 2 cut(s) 440, 662
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 1 cut(s) 1312
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 4 cut(s) 523, 550, 1375, 1576
BtsI GCAGTG 2 cut(s) 347, 1083
BtsIMutI CAGTG 2 cut(s) 347, 1083
BveI ACCTGC 2 cut(s) 850, 1526
Cac8I GCNNGC 5 cut(s) 7, 97, 333, 789, 1188
CciI TCATGA 2 cut(s) 181, 919
CfoI GCGC 2 cut(s) 1717, 1738
Cfr13I GGNCC 1 cut(s) 1775
Csp6I GTAC 3 cut(s) 522, 1683, 1796
CviAII CATG 4 cut(s) 182, 920, 1016, 1301
CviQI GTAC 3 cut(s) 522, 1683, 1796
DdeI CTNAG 6 cut(s) 381, 421, 792, 885, 1305, 1320
DpnI GATC 8 cut(s) 22, 107, 186, 442, 463, 536, 664, 1593
DpnII GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
DraIII CACNNNGTG 1 cut(s) 61
Eam1104I CTCTTC 3 cut(s) 767, 1257, 1675
EarI CTCTTC 3 cut(s) 767, 1257, 1675
Eco130I CCWWGG 2 cut(s) 991, 1694
Eco147I AGGCCT 1 cut(s) 1312
Eco32I GATATC 1 cut(s) 1609
Eco47I GGWCC 1 cut(s) 1775
Eco57I CTGAAG 3 cut(s) 699, 957, 1656
Eco81I CCTNAGG 1 cut(s) 885
EcoRII CCWGG 2 cut(s) 540, 638
EcoRV GATATC 1 cut(s) 1609
EcoT14I CCWWGG 2 cut(s) 991, 1694
ErhI CCWWGG 2 cut(s) 991, 1694
FaeI CATG 4 cut(s) 185, 923, 1019, 1304
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 2 cut(s) 231, 1788
FatI CATG 4 cut(s) 181, 919, 1015, 1300
FblI GTMKAC 1 cut(s) 955
Fnu4HI GCNGC 4 cut(s) 1262, 1503, 1602, 1713
FokI GGATG 4 cut(s) 530, 557, 1382, 1583
Fsp4HI GCNGC 4 cut(s) 1262, 1503, 1602, 1713
FspBI CTAG 8 cut(s) 444, 984, 1041, 1220, 1293, 1406, 1695, 1819
GlaI GCGC 2 cut(s) 1716, 1737
GluI GCNGC 4 cut(s) 1262, 1503, 1602, 1713
GsuI CTGGAG 1 cut(s) 1295
HaeIII GGCC 1 cut(s) 1312
HapII CCGG 3 cut(s) 365, 816, 1420
HhaI GCGC 2 cut(s) 1717, 1738
Hin1II CATG 4 cut(s) 185, 923, 1019, 1304
Hin6I GCGC 2 cut(s) 1715, 1736
HinP1I GCGC 2 cut(s) 1715, 1736
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 5 cut(s) 410, 674, 805, 916, 1162
HpaII CCGG 3 cut(s) 365, 816, 1420
HphI GGTGA 5 cut(s) 73, 925, 1007, 1171, 1445
Hpy166II GTNNAC 2 cut(s) 956, 1796
Hpy8I GTNNAC 2 cut(s) 956, 1796
HpyAV CCTTC 3 cut(s) 1170, 1350, 1619
HpyCH4III ACNGT 3 cut(s) 471, 769, 1290
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 9 cut(s) 244, 396, 1076, 1124, 1202, 1261, 1505, 1601, 1751
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 337, 987, 1733
HpyF3I CTNAG 6 cut(s) 381, 421, 792, 885, 1305, 1320
HpySE526I ACGT 1 cut(s) 952
Hsp92II CATG 4 cut(s) 185, 923, 1019, 1304
HspAI GCGC 2 cut(s) 1715, 1736
Kpn2I TCCGGA 1 cut(s) 1419
Kzo9I GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
LguI GCTCTTC 1 cut(s) 1257
LmnI GCTCC 1 cut(s) 1409
Lsp1109I GCAGC 4 cut(s) 1273, 1489, 1613, 1724
LweI GCATC 4 cut(s) 535, 977, 1189, 1561
MaeI CTAG 8 cut(s) 444, 984, 1041, 1220, 1293, 1406, 1695, 1819
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 3 cut(s) 87, 945, 1810
MalI GATC 8 cut(s) 22, 107, 186, 442, 463, 536, 664, 1593
MboI GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
MboII GAAGA 6 cut(s) 405, 784, 963, 1244, 1652, 1662
MfeI CAATTG 2 cut(s) 1256, 1612
MflI RGATCY 2 cut(s) 440, 662
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 3 cut(s) 50, 326, 825
MroI TCCGGA 1 cut(s) 1419
MseI TTAA 5 cut(s) 318, 603, 623, 894, 1397
MslI CAYNNNNRTG 2 cut(s) 454, 469
MspI CCGG 3 cut(s) 365, 816, 1420
MspR9I CCNGG 2 cut(s) 542, 640
MunI CAATTG 2 cut(s) 1256, 1612
Mva1269I GAATGC 1 cut(s) 1603
MvaI CCWGG 2 cut(s) 542, 640
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 4 cut(s) 332, 337, 987, 1733
NdeII GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
NlaIII CATG 4 cut(s) 185, 923, 1019, 1304
NlaIV GGNNCC 4 cut(s) 555, 637, 814, 1776
NmuCI GTSAC 1 cut(s) 87
NspI RCATGY 1 cut(s) 1019
PagI TCATGA 2 cut(s) 181, 919
PceI AGGCCT 1 cut(s) 1312
PciSI GCTCTTC 1 cut(s) 1257
PctI GAATGC 1 cut(s) 1603
PfeI GAWTC 4 cut(s) 410, 805, 916, 1162
PfoI TCCNGGA 1 cut(s) 638
PkrI GCNGC 4 cut(s) 1263, 1504, 1603, 1714
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 540, 638
PspGI CCWGG 2 cut(s) 540, 638
PspN4I GGNNCC 4 cut(s) 555, 637, 814, 1776
PspPI GGNCC 1 cut(s) 1775
PsrI GAACNNNNNNTAC 2 cut(s) 1371, 1403
PstI CTGCAG 1 cut(s) 1507
PsuI RGATCY 2 cut(s) 440, 662
RsaI GTAC 3 cut(s) 523, 1684, 1797
RsaNI GTAC 3 cut(s) 522, 1683, 1796
RseI CAYNNNNRTG 2 cut(s) 454, 469
SapI GCTCTTC 1 cut(s) 1257
SaqAI TTAA 5 cut(s) 318, 603, 623, 894, 1397
SatI GCNGC 4 cut(s) 1262, 1503, 1602, 1713
Sau3AI GATC 8 cut(s) 20, 105, 184, 440, 461, 534, 662, 1591
Sau96I GGNCC 1 cut(s) 1775
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 542, 640
SfaNI GCATC 4 cut(s) 535, 977, 1189, 1561
SfcI CTRYAG 1 cut(s) 1503
SinI GGWCC 1 cut(s) 1775
SmiMI CAYNNNNRTG 2 cut(s) 454, 469
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
SpeI ACTAGT 1 cut(s) 1219
SseBI AGGCCT 1 cut(s) 1312
SsiI CCGC 3 cut(s) 205, 1448, 1660
SspI AATATT 1 cut(s) 401
SspMI CTAG 8 cut(s) 444, 984, 1041, 1220, 1293, 1406, 1695, 1819
StuI AGGCCT 1 cut(s) 1312
StyD4I CCNGG 2 cut(s) 540, 638
StyI CCWWGG 2 cut(s) 991, 1694
TaaI ACNGT 3 cut(s) 471, 769, 1290
TaiI ACGT 1 cut(s) 955
TaqI TCGA 4 cut(s) 146, 808, 1547, 1619
TatI WGTACW 2 cut(s) 1682, 1795
TfiI GAWTC 4 cut(s) 410, 805, 916, 1162
Tru1I TTAA 5 cut(s) 318, 603, 623, 894, 1397
Tru9I TTAA 5 cut(s) 318, 603, 623, 894, 1397
TscAI CASTG 2 cut(s) 347, 1083
TseFI GTSAC 1 cut(s) 87
TseI GCWGC 4 cut(s) 1261, 1502, 1601, 1712
Tsp45I GTSAC 1 cut(s) 87
TspDTI ATGAA 3 cut(s) 908, 1161, 1440
TspRI CASTG 2 cut(s) 347, 1083
VpaK11BI GGWCC 1 cut(s) 1775
XapI RAATTY 2 cut(s) 879, 1099
XbaI TCTAGA 1 cut(s) 983
XceI RCATGY 1 cut(s) 1019
XmaJI CCTAGG 1 cut(s) 1694
XmiI GTMKAC 1 cut(s) 955
XspI CTAG 8 cut(s) 444, 984, 1041, 1220, 1293, 1406, 1695, 1819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.