Rroxscaffold_6G00410800

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001470
Physical Location & Seq
Reverse (-)
33210705 .. 33212877
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410800.1

Sequence Viewer

Length: 837 bp
ATGGAGTATACTTTGTCAGGTTTTAGAGAATTTGTCGATTTTAATCAAGCTCTTCTGGCTAAACAAGCATGGAGATTAATTTCATACCCAGATTCCCTAGTGGCTCGGATTTACAGAAGCCACTACTACCCACGCACTTCTTTCCTCAACTCCCATTTTGGCAGGTCACCATCTTTCATTTGGAGGTCCATTTTATGGGGAAGTGAACTTCTTGTTTCTGGTCTAAGATGGAAAGTTGGGGATGGAAGATCAATCTCAATCTACAGAGATGCTTGGATACCAATACCTCACTCTTTTCGTATACAATCTCCACCTGGTATTTTATTCAATTCTTCTGTTGCTAGTCTCTTTGGCGCTGATGGCTATTGGAATTATGATCTGATCATACATACCTTTTGGGTGTTGGAGGCTGAAGCAATATTATCCATTCCTTTACCTTTTTCCCCGGCTTCAGATATGTTATGTTGGCATTATACCAAGAATGACTTGTATACGGTAAAGAGTGGCTACAAACTGATCTTAAAGAAGAGAGATTGGCAAAATGGTGGTAAGGGCAATTCTATCTCACAAGATAACATTTGGGGATGTATTTGGAGACTTAAAACCCCGGCTGCAGCTAGAATTTTCTTTTGGCGAGCTGTAAATGAAATTTTTCCTTGTGCGGTCAACTACACGAGCGACATTTGCTTTCAACCCCAATCTGCTCTCGCTGCTTCTTATTTCGAGAGACAACAATTCATGCTTTATAGTCTTGTTCGAAGGCTAAGGAGGTTTGGAAGCTCTCCCCTTTTAGGAATTCAGTTGGCAATTGGAAGCAGGGCACCTTCAAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

278

Amino Acids

32.07

Weight (kDa)

9.76

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 153
AccB1I GGYRCC 1 cut(s) 820
AccB7I CCANNNNNTGG 1 cut(s) 195
AccI GTMKAC 3 cut(s) 8, 301, 491
AciI CCGC 1 cut(s) 662
AcsI RAATTY 4 cut(s) 29, 621, 648, 795
AcuI CTGAAG 2 cut(s) 432, 435
AfiI CCNNNNNNNGG 2 cut(s) 195, 791
AgsI TTSAA 3 cut(s) 328, 692, 828
AjnI CCWGG 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 4 cut(s) 50, 617, 638, 780
AluI AGCT 4 cut(s) 50, 617, 638, 780
Alw26I GTCTC 3 cut(s) 350, 589, 721
ApeKI GCWGC 3 cut(s) 611, 614, 710
ApoI RAATTY 4 cut(s) 29, 621, 648, 795
AseI ATTAAT 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 2 cut(s) 186, 831
AsuC2I CCSGG 2 cut(s) 446, 608
AsuHPI GGTGA 1 cut(s) 159
AsuII TTCGAA 1 cut(s) 757
AvaII GGWCC 2 cut(s) 186, 831
BaeGI GKGCMC 1 cut(s) 823
BanI GGYRCC 1 cut(s) 820
BauI CACGAG 1 cut(s) 673
BbvI GCAGC 3 cut(s) 598, 626, 697
BccI CCATC 4 cut(s) 178, 222, 236, 353
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 2 cut(s) 446, 608
BcoDI GTCTC 3 cut(s) 350, 589, 721
BfaI CTAG 4 cut(s) 98, 342, 618, 835
BfmI CTRYAG 2 cut(s) 262, 612
BfoI RGCGCY 1 cut(s) 357
BfuAI ACCTGC 1 cut(s) 153
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 3 cut(s) 612, 615, 711
BlsI GCNGC 3 cut(s) 613, 616, 712
Bme1390I CCNGG 3 cut(s) 315, 446, 608
Bme18I GGWCC 2 cut(s) 186, 831
BmgT120I GGNCC 2 cut(s) 186, 831
BmiI GGNNCC 1 cut(s) 822
BmrFI CCNGG 3 cut(s) 315, 446, 608
BmsI GCATC 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 764
Bpu14I TTCGAA 1 cut(s) 757
BpuMI CCSGG 2 cut(s) 446, 608
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 444, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 791
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 444, 606
BseGI GGATG 2 cut(s) 247, 590
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 195, 791
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 3 cut(s) 598, 626, 697
BshNI GGYRCC 1 cut(s) 820
BsiSI CCGG 2 cut(s) 446, 608
BslI CCNNNNNNNGG 2 cut(s) 195, 791
BsmAI GTCTC 3 cut(s) 350, 589, 721
Bsp119I TTCGAA 1 cut(s) 757
Bsp1286I GDGCHC 1 cut(s) 823
Bsp143I GATC 4 cut(s) 248, 376, 381, 516
BspACI CCGC 1 cut(s) 662
BspLI GGNNCC 1 cut(s) 822
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 153
BspQI GCTCTTC 1 cut(s) 57
BspT104I TTCGAA 1 cut(s) 757
BspT107I GGYRCC 1 cut(s) 820
BssECI CCNNGG 2 cut(s) 444, 606
BssMI GATC 4 cut(s) 248, 376, 381, 516
BssNAI GTATAC 3 cut(s) 9, 302, 492
BssSI CACGAG 1 cut(s) 673
Bst1107I GTATAC 3 cut(s) 9, 302, 492
Bst2BI CACGAG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 2 cut(s) 57, 521
BstBI TTCGAA 1 cut(s) 757
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 2 cut(s) 224, 764
BstEII GGTNACC 1 cut(s) 165
BstF5I GGATG 2 cut(s) 247, 590
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 4 cut(s) 251, 379, 384, 519
BstMAI GTCTC 3 cut(s) 350, 589, 721
BstMBI GATC 4 cut(s) 248, 376, 381, 516
BstMWI GCNNNNNNNGC 5 cut(s) 56, 65, 360, 684, 710
BstNI CCWGG 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 165
BstSCI CCNGG 3 cut(s) 313, 444, 606
BstSFI CTRYAG 2 cut(s) 262, 612
BstSLI GKGCMC 1 cut(s) 823
BstV1I GCAGC 3 cut(s) 598, 626, 697
BstZ17I GTATAC 3 cut(s) 9, 302, 492
BsuI GTATCC 1 cut(s) 270
BtsCI GGATG 2 cut(s) 247, 590
BveI ACCTGC 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 186, 831
CsiI ACCWGGT 1 cut(s) 313
CviAII CATG 2 cut(s) 69, 739
DdeI CTNAG 2 cut(s) 224, 764
DpnI GATC 4 cut(s) 250, 378, 383, 518
DpnII GATC 4 cut(s) 248, 376, 381, 516
Eam1104I CTCTTC 2 cut(s) 57, 521
EarI CTCTTC 2 cut(s) 57, 521
Eco47I GGWCC 2 cut(s) 186, 831
Eco57I CTGAAG 2 cut(s) 432, 435
Eco91I GGTNACC 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 831
EcoO65I GGTNACC 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 795
EcoRII CCWGG 1 cut(s) 313
FaeI CATG 2 cut(s) 72, 742
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FatI CATG 2 cut(s) 68, 738
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 3 cut(s) 8, 301, 491
Fnu4HI GCNGC 3 cut(s) 612, 615, 711
FokI GGATG 2 cut(s) 254, 597
Fsp4HI GCNGC 3 cut(s) 612, 615, 711
FspBI CTAG 4 cut(s) 98, 342, 618, 835
GlaI GCGC 1 cut(s) 355
GluI GCNGC 3 cut(s) 612, 615, 711
HaeII RGCGCY 1 cut(s) 357
HapII CCGG 2 cut(s) 446, 608
HhaI GCGC 1 cut(s) 356
Hin1II CATG 2 cut(s) 72, 742
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 1 cut(s) 92
HpaII CCGG 2 cut(s) 446, 608
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 5 cut(s) 9, 206, 302, 492, 667
Hpy188I TCNGA 3 cut(s) 108, 381, 454
Hpy188III TCNNGA 1 cut(s) 724
Hpy8I GTNNAC 5 cut(s) 9, 206, 302, 492, 667
HpyAV CCTTC 2 cut(s) 753, 834
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4V TGCA 1 cut(s) 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 65, 360, 684, 710
HpyF3I CTNAG 2 cut(s) 224, 764
Hsp92II CATG 2 cut(s) 72, 742
HspAI GCGC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 4 cut(s) 248, 376, 381, 516
LguI GCTCTTC 1 cut(s) 57
Lsp1109I GCAGC 3 cut(s) 598, 626, 697
LweI GCATC 1 cut(s) 259
MabI ACCWGGT 1 cut(s) 313
MaeI CTAG 4 cut(s) 98, 342, 618, 835
MaeIII GTNAC 1 cut(s) 165
MalI GATC 4 cut(s) 250, 378, 383, 518
MboI GATC 4 cut(s) 248, 376, 381, 516
MboII GAAGA 4 cut(s) 44, 258, 324, 538
MfeI CAATTG 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 5 cut(s) 155, 177, 297, 400, 762
MroXI GAANNNNTTC 1 cut(s) 651
MseI TTAA 4 cut(s) 42, 77, 521, 600
MspI CCGG 2 cut(s) 446, 608
MspR9I CCNGG 3 cut(s) 315, 446, 608
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 5 cut(s) 56, 65, 360, 684, 710
NciI CCSGG 2 cut(s) 446, 608
NdeII GATC 4 cut(s) 248, 376, 381, 516
NlaIII CATG 2 cut(s) 72, 742
NlaIV GGNNCC 1 cut(s) 822
NmuCI GTSAC 1 cut(s) 165
NspV TTCGAA 1 cut(s) 757
PciSI GCTCTTC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 195
PkrI GCNGC 3 cut(s) 613, 616, 712
PpuMI RGGWCCY 1 cut(s) 831
PshBI ATTAAT 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 165
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 822
PspPI GGNCC 2 cut(s) 186, 831
PspPPI RGGWCCY 1 cut(s) 831
PstI CTGCAG 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 4 cut(s) 42, 77, 521, 600
SatI GCNGC 3 cut(s) 612, 615, 711
Sau3AI GATC 4 cut(s) 248, 376, 381, 516
Sau96I GGNCC 2 cut(s) 186, 831
ScrFI CCNGG 3 cut(s) 315, 446, 608
SduI GDGCHC 1 cut(s) 823
SexAI ACCWGGT 1 cut(s) 313
SfaNI GCATC 1 cut(s) 259
SfcI CTRYAG 2 cut(s) 262, 612
SfuI TTCGAA 1 cut(s) 757
SinI GGWCC 2 cut(s) 186, 831
SsiI CCGC 1 cut(s) 662
SspI AATATT 1 cut(s) 420
SspMI CTAG 4 cut(s) 98, 342, 618, 835
StyD4I CCNGG 3 cut(s) 313, 444, 606
TaaI ACNGT 1 cut(s) 496
TaqI TCGA 3 cut(s) 36, 723, 757
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 4 cut(s) 42, 77, 521, 600
Tru9I TTAA 4 cut(s) 42, 77, 521, 600
TseFI GTSAC 1 cut(s) 165
TseI GCWGC 3 cut(s) 611, 614, 710
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 4 cut(s) 72, 166, 660, 727
Van91I CCANNNNNTGG 1 cut(s) 195
VpaK11BI GGWCC 2 cut(s) 186, 831
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 4 cut(s) 29, 621, 648, 795
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XmiI GTMKAC 3 cut(s) 8, 301, 491
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 4 cut(s) 98, 342, 618, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.