Rmu_sc0005933.1_g000002

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005933.1
OG0001470
Physical Location & Seq
Forward (+)
9182 .. 10178
997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005933.1_g000002.1.cds

Sequence Viewer

Length: 690 bp
atgatgctttttggaggcaatgatgttaaggttggggtttcatctcttatgggagaaacgtattatggattgcttgataacggtttccttttcagtgttgtggaaagggaatcctttagggtactttgttccatcacgaggaatcaagcagggctgtcctttgtcaccatgcttgtttctctttgtggcagaaggcttctcaaggctgctccagcaggcaaaggctgcaggaaagatttatggtgttcgaatttcaaggacggaatttggcacaagataaatggctggaaggaaaagattctttcccaggcgggtaaagagattattctaaaatcagtggttcaagctattccatcttcctcaatgagtgtgttttggatgcttgtgactttgagtcatgaactatcaagtttatcagccaggttttggtgggggaggcagaaaggtaaacgtggtcttcattggctacggtgggattctttatgtagaaacaagttgaaaggtgggctaggctttcgagattttgttaagttcagtcaagcactcttggcccaacaatgttggagattgttctctcttcctgatttgttggtcacgaaggtgtataaagggcgttactatccttcttcctctcttctatctgcacctgttgggaggtcaagctcactgatatggaggtccctcctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

229

Amino Acids

25.88

Weight (kDa)

10.17

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 426
AciI CCGC 1 cut(s) 311
AcsI RAATTY 2 cut(s) 250, 264
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 138, 426
AgsI TTSAA 3 cut(s) 256, 344, 499
AhdI GACNNNNNGTC 1 cut(s) 393
AjnI CCWGG 2 cut(s) 306, 419
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 2 cut(s) 347, 663
AluI AGCT 2 cut(s) 347, 663
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 2 cut(s) 206, 225
ApoI RAATTY 2 cut(s) 250, 264
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 550, 678
AsuHPI GGTGA 1 cut(s) 157
AsuII TTCGAA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 678
BauI CACGAG 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 449
BbvI GCAGC 2 cut(s) 193, 212
BccI CCATC 2 cut(s) 140, 361
BciT130I CCWGG 2 cut(s) 308, 421
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 226
BisI GCNGC 2 cut(s) 207, 226
BlsI GCNGC 2 cut(s) 208, 227
Bme1390I CCNGG 2 cut(s) 308, 421
Bme18I GGWCC 1 cut(s) 678
BmeRI GACNNNNNGTC 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 550, 678
BmiI GGNNCC 1 cut(s) 680
BmrFI CCNGG 2 cut(s) 308, 421
BmsI GCATC 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 426
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 2 cut(s) 308, 421
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 384
BseLI CCNNNNNNNGG 2 cut(s) 138, 426
BseMI GCAATG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 193, 212
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 1 cut(s) 551
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 2 cut(s) 138, 426
BsmFI GGGAC 1 cut(s) 664
BsnI GGCC 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 248
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 551
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 1 cut(s) 680
BspMAI CTGCAG 1 cut(s) 230
BspT104I TTCGAA 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 306
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst2UI CCWGG 2 cut(s) 308, 421
Bst4CI ACNGT 2 cut(s) 83, 471
Bst6I CTCTTC 2 cut(s) 582, 639
BstAPI GCANNNNNTGC 1 cut(s) 225
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 217
BstF5I GGATG 1 cut(s) 384
BstMWI GCNNNNNNNGC 3 cut(s) 212, 225, 548
BstNI CCWGG 2 cut(s) 308, 421
BstSCI CCNGG 2 cut(s) 306, 419
BstSFI CTRYAG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 193, 212
BstV2I GAAGAC 1 cut(s) 449
BsuRI GGCC 1 cut(s) 551
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 3 cut(s) 100, 342, 665
Cac8I GCNNGC 1 cut(s) 217
CciI TCATGA 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 550, 678
Csp6I GTAC 1 cut(s) 122
CviAII CATG 2 cut(s) 169, 398
CviQI GTAC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 582, 639
Eam1105I GACNNNNNGTC 1 cut(s) 393
EarI CTCTTC 2 cut(s) 582, 639
Eco47I GGWCC 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 678
EcoRII CCWGG 2 cut(s) 306, 419
FaeI CATG 2 cut(s) 172, 401
FaiI YATR 8 cut(s) 50, 66, 170, 241, 399, 484, 606, 673
FaqI GGGAC 1 cut(s) 664
FatI CATG 2 cut(s) 168, 397
FauI CCCGC 1 cut(s) 304
Fnu4HI GCNGC 2 cut(s) 207, 226
FokI GGATG 1 cut(s) 391
Fsp4HI GCNGC 2 cut(s) 207, 226
FspBI CTAG 1 cut(s) 509
GluI GCNGC 2 cut(s) 207, 226
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 1 cut(s) 551
Hin1II CATG 2 cut(s) 172, 401
HinfI GANTC 5 cut(s) 110, 142, 298, 394, 476
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 449
Hpy188III TCNNGA 5 cut(s) 136, 398, 518, 581, 595
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 4 cut(s) 186, 283, 592, 633
HpyCH4III ACNGT 2 cut(s) 83, 471
HpyCH4IV ACGT 2 cut(s) 59, 451
HpyCH4V TGCA 2 cut(s) 228, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 225, 548
HpySE526I ACGT 2 cut(s) 59, 451
Hsp92II CATG 2 cut(s) 172, 401
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 2 cut(s) 193, 212
LweI GCATC 1 cut(s) 369
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 59, 451
MaeIII GTNAC 4 cut(s) 163, 385, 592, 614
MboII GAAGA 5 cut(s) 348, 449, 569, 618, 626
MluCI AATT 2 cut(s) 250, 264
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 7 cut(s) 8, 132, 370, 429, 640, 648, 669
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 2 cut(s) 27, 528
MslI CAYNNNNRTG 2 cut(s) 599, 670
MspR9I CCNGG 2 cut(s) 308, 421
MvaI CCWGG 2 cut(s) 308, 421
MwoI GCNNNNNNNGC 3 cut(s) 212, 225, 548
NlaIII CATG 2 cut(s) 172, 401
NlaIV GGNNCC 1 cut(s) 680
NmuCI GTSAC 3 cut(s) 163, 385, 592
NspV TTCGAA 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 110, 142, 298, 476
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 2 cut(s) 208, 227
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
PpuMI RGGWCCY 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 678
Psp6I CCWGG 2 cut(s) 306, 419
PspGI CCWGG 2 cut(s) 306, 419
PspN4I GGNNCC 1 cut(s) 680
PspPI GGNCC 2 cut(s) 550, 678
PspPPI RGGWCCY 1 cut(s) 678
PstI CTGCAG 1 cut(s) 230
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 2 cut(s) 599, 670
SaqAI TTAA 2 cut(s) 27, 528
SatI GCNGC 2 cut(s) 207, 226
Sau96I GGNCC 2 cut(s) 550, 678
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 308, 421
SfaNI GCATC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 248
SinI GGWCC 1 cut(s) 678
SmiMI CAYNNNNRTG 2 cut(s) 599, 670
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 250, 264
SsiI CCGC 1 cut(s) 311
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 306, 419
TaaI ACNGT 2 cut(s) 83, 471
TaiI ACGT 2 cut(s) 62, 454
TaqI TCGA 2 cut(s) 248, 517
TasI AATT 2 cut(s) 250, 264
TfiI GAWTC 4 cut(s) 110, 142, 298, 476
Tru1I TTAA 2 cut(s) 27, 528
Tru9I TTAA 2 cut(s) 27, 528
TscAI CASTG 3 cut(s) 100, 342, 672
TseFI GTSAC 3 cut(s) 163, 385, 592
TseI GCWGC 2 cut(s) 206, 225
Tsp45I GTSAC 3 cut(s) 163, 385, 592
TspDTI ATGAA 3 cut(s) 30, 414, 449
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 3 cut(s) 100, 342, 672
Van91I CCANNNNNTGG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 678
XapI RAATTY 2 cut(s) 250, 264
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.