Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 466 |
| AclWI | GGATC | 4 cut(s) | 365, 406, 419, 717 |
| AcsI | RAATTY | 1 cut(s) | 40 |
| AcuI | CTGAAG | 3 cut(s) | 376, 645, 750 |
| AcvI | CACGTG | 1 cut(s) | 772 |
| AdeI | CACNNNGTG | 1 cut(s) | 182 |
| AfaI | GTAC | 3 cut(s) | 423, 428, 666 |
| AflII | CTTAAG | 1 cut(s) | 140 |
| AgsI | TTSAA | 7 cut(s) | 149, 368, 449, 461, 647, 737, 746 |
| AluBI | AGCT | 2 cut(s) | 145, 356 |
| AluI | AGCT | 2 cut(s) | 145, 356 |
| Alw26I | GTCTC | 2 cut(s) | 129, 231 |
| AlwI | GGATC | 4 cut(s) | 365, 406, 419, 717 |
| AoxI | GGCC | 1 cut(s) | 112 |
| ApeKI | GCWGC | 2 cut(s) | 168, 702 |
| ApoI | RAATTY | 1 cut(s) | 40 |
| AseI | ATTAAT | 1 cut(s) | 405 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 630 |
| AspS9I | GGNCC | 2 cut(s) | 75, 274 |
| AsuHPI | GGTGA | 2 cut(s) | 322, 767 |
| AvaII | GGWCC | 2 cut(s) | 75, 274 |
| AxyI | CCTNAGG | 1 cut(s) | 270 |
| BamHI | GGATCC | 1 cut(s) | 411 |
| BauI | CACGAG | 1 cut(s) | 180 |
| BbrPI | CACGTG | 1 cut(s) | 772 |
| BbsI | GAAGAC | 1 cut(s) | 744 |
| BbvI | GCAGC | 2 cut(s) | 180, 714 |
| BccI | CCATC | 5 cut(s) | 158, 290, 565, 619, 692 |
| BcoDI | GTCTC | 2 cut(s) | 129, 231 |
| BfaI | CTAG | 1 cut(s) | 347 |
| BfmI | CTRYAG | 2 cut(s) | 221, 243 |
| BfrI | CTTAAG | 1 cut(s) | 140 |
| BisI | GCNGC | 2 cut(s) | 169, 703 |
| BlsI | GCNGC | 2 cut(s) | 170, 704 |
| BmcAI | AGTACT | 1 cut(s) | 428 |
| Bme18I | GGWCC | 2 cut(s) | 75, 274 |
| BmgT120I | GGNCC | 2 cut(s) | 75, 274 |
| BmiI | GGNNCC | 1 cut(s) | 413 |
| BmsI | GCATC | 3 cut(s) | 333, 697, 715 |
| BpiI | GAAGAC | 1 cut(s) | 744 |
| Bsa29I | ATCGAT | 1 cut(s) | 696 |
| BsaAI | YACGTR | 1 cut(s) | 772 |
| BsaI | GGTCTC | 1 cut(s) | 129 |
| BsaJI | CCNNGG | 3 cut(s) | 415, 539, 561 |
| Bse21I | CCTNAGG | 1 cut(s) | 270 |
| Bse3DI | GCAATG | 1 cut(s) | 601 |
| BseCI | ATCGAT | 1 cut(s) | 696 |
| BseDI | CCNNGG | 3 cut(s) | 415, 539, 561 |
| BseGI | GGATG | 1 cut(s) | 730 |
| BseMI | GCAATG | 1 cut(s) | 601 |
| BseMII | CTCAG | 3 cut(s) | 242, 261, 494 |
| BseXI | GCAGC | 2 cut(s) | 180, 714 |
| BshFI | GGCC | 1 cut(s) | 114 |
| BshVI | ATCGAT | 1 cut(s) | 696 |
| BslFI | GGGAC | 1 cut(s) | 655 |
| BsmAI | GTCTC | 2 cut(s) | 129, 231 |
| BsmFI | GGGAC | 1 cut(s) | 655 |
| BsnI | GGCC | 1 cut(s) | 114 |
| Bso31I | GGTCTC | 1 cut(s) | 129 |
| Bsp143I | GATC | 4 cut(s) | 370, 411, 439, 709 |
| Bsp19I | CCATGG | 1 cut(s) | 561 |
| BspANI | GGCC | 1 cut(s) | 114 |
| BspCNI | CTCAG | 3 cut(s) | 241, 262, 493 |
| BspDI | ATCGAT | 1 cut(s) | 696 |
| BspLI | GGNNCC | 1 cut(s) | 413 |
| BspMAI | CTGCAG | 1 cut(s) | 225 |
| BspPI | GGATC | 4 cut(s) | 365, 406, 419, 717 |
| BspTI | CTTAAG | 1 cut(s) | 140 |
| BspTNI | GGTCTC | 1 cut(s) | 129 |
| BsrDI | GCAATG | 1 cut(s) | 601 |
| BssECI | CCNNGG | 3 cut(s) | 415, 539, 561 |
| BssMI | GATC | 4 cut(s) | 370, 411, 439, 709 |
| BssSI | CACGAG | 1 cut(s) | 180 |
| BssT1I | CCWWGG | 2 cut(s) | 415, 561 |
| Bst2BI | CACGAG | 1 cut(s) | 180 |
| Bst4CI | ACNGT | 3 cut(s) | 244, 426, 669 |
| BstAFI | CTTAAG | 1 cut(s) | 140 |
| BstBAI | YACGTR | 1 cut(s) | 772 |
| BstDEI | CTNAG | 4 cut(s) | 160, 228, 270, 480 |
| BstDSI | CCRYGG | 2 cut(s) | 539, 561 |
| BstF5I | GGATG | 1 cut(s) | 730 |
| BstKTI | GATC | 4 cut(s) | 373, 414, 442, 712 |
| BstMAI | GTCTC | 2 cut(s) | 129, 231 |
| BstMBI | GATC | 4 cut(s) | 370, 411, 439, 709 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 229, 263 |
| BstSFI | CTRYAG | 2 cut(s) | 221, 243 |
| BstV1I | GCAGC | 2 cut(s) | 180, 714 |
| BstV2I | GAAGAC | 1 cut(s) | 744 |
| BstX2I | RGATCY | 3 cut(s) | 370, 411, 709 |
| BstYI | RGATCY | 3 cut(s) | 370, 411, 709 |
| Bsu15I | ATCGAT | 1 cut(s) | 696 |
| Bsu36I | CCTNAGG | 1 cut(s) | 270 |
| BsuRI | GGCC | 1 cut(s) | 114 |
| BsuTUI | ATCGAT | 1 cut(s) | 696 |
| BtgI | CCRYGG | 2 cut(s) | 539, 561 |
| BtsCI | GGATG | 1 cut(s) | 730 |
| BtsIMutI | CAGTG | 1 cut(s) | 621 |
| Cfr13I | GGNCC | 2 cut(s) | 75, 274 |
| ClaI | ATCGAT | 1 cut(s) | 696 |
| Csp6I | GTAC | 3 cut(s) | 422, 427, 665 |
| CviAII | CATG | 3 cut(s) | 443, 562, 706 |
| CviJI | RGCY | 5 cut(s) | 114, 145, 232, 257, 356 |
| CviKI_1 | RGCY | 5 cut(s) | 114, 145, 232, 257, 356 |
| CviQI | GTAC | 3 cut(s) | 422, 427, 665 |
| DdeI | CTNAG | 4 cut(s) | 160, 228, 270, 480 |
| DpnI | GATC | 4 cut(s) | 372, 413, 441, 711 |
| DpnII | GATC | 4 cut(s) | 370, 411, 439, 709 |
| DraIII | CACNNNGTG | 1 cut(s) | 182 |
| Eco130I | CCWWGG | 2 cut(s) | 415, 561 |
| Eco31I | GGTCTC | 1 cut(s) | 129 |
| Eco47I | GGWCC | 2 cut(s) | 75, 274 |
| Eco57I | CTGAAG | 3 cut(s) | 376, 645, 750 |
| Eco72I | CACGTG | 1 cut(s) | 772 |
| Eco81I | CCTNAGG | 1 cut(s) | 270 |
| EcoO109I | RGGNCCY | 1 cut(s) | 274 |
| EcoT14I | CCWWGG | 2 cut(s) | 415, 561 |
| ErhI | CCWWGG | 2 cut(s) | 415, 561 |
| FaeI | CATG | 3 cut(s) | 446, 565, 709 |
| FaqI | GGGAC | 1 cut(s) | 655 |
| FatI | CATG | 3 cut(s) | 442, 561, 705 |
| FblI | GTMKAC | 1 cut(s) | 466 |
| Fnu4HI | GCNGC | 2 cut(s) | 169, 703 |
| FokI | GGATG | 1 cut(s) | 737 |
| Fsp4HI | GCNGC | 2 cut(s) | 169, 703 |
| FspBI | CTAG | 1 cut(s) | 347 |
| GluI | GCNGC | 2 cut(s) | 169, 703 |
| HaeIII | GGCC | 1 cut(s) | 114 |
| Hin1II | CATG | 3 cut(s) | 446, 565, 709 |
| HindIII | AAGCTT | 1 cut(s) | 143 |
| HinfI | GANTC | 1 cut(s) | 327 |
| HphI | GGTGA | 2 cut(s) | 322, 767 |
| Hpy166II | GTNNAC | 2 cut(s) | 75, 467 |
| Hpy188I | TCNGA | 4 cut(s) | 65, 395, 411, 483 |
| Hpy188III | TCNNGA | 5 cut(s) | 269, 374, 437, 647, 713 |
| Hpy8I | GTNNAC | 2 cut(s) | 75, 467 |
| HpyAV | CCTTC | 1 cut(s) | 569 |
| HpyCH4III | ACNGT | 3 cut(s) | 244, 426, 669 |
| HpyCH4IV | ACGT | 1 cut(s) | 771 |
| HpyCH4V | TGCA | 2 cut(s) | 223, 308 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 229, 263 |
| HpyF3I | CTNAG | 4 cut(s) | 160, 228, 270, 480 |
| HpySE526I | ACGT | 1 cut(s) | 771 |
| Hsp92II | CATG | 3 cut(s) | 446, 565, 709 |
| Kzo9I | GATC | 4 cut(s) | 370, 411, 439, 709 |
| LmnI | GCTCC | 2 cut(s) | 271, 353 |
| LpnPI | CCDG | 8 cut(s) | 21, 91, 128, 267, 282, 387, 450, 507 |
| Lsp1109I | GCAGC | 2 cut(s) | 180, 714 |
| LweI | GCATC | 3 cut(s) | 333, 697, 715 |
| MaeI | CTAG | 1 cut(s) | 347 |
| MaeII | ACGT | 1 cut(s) | 771 |
| MaeIII | GTNAC | 5 cut(s) | 203, 213, 328, 682, 767 |
| MalI | GATC | 4 cut(s) | 372, 413, 441, 711 |
| MboI | GATC | 4 cut(s) | 370, 411, 439, 709 |
| MboII | GAAGA | 3 cut(s) | 380, 638, 749 |
| MflI | RGATCY | 3 cut(s) | 370, 411, 709 |
| MluCI | AATT | 6 cut(s) | 40, 303, 402, 432, 525, 642 |
| MlyI | GAGTC | 1 cut(s) | 336 |
| MmeI | TCCRAC | 1 cut(s) | 331 |
| MnlI | CCTC | 5 cut(s) | 104, 189, 265, 572, 643 |
| MroXI | GAANNNNTTC | 1 cut(s) | 630 |
| MseI | TTAA | 5 cut(s) | 96, 141, 405, 528, 781 |
| MspCI | CTTAAG | 1 cut(s) | 140 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 229, 263 |
| NcoI | CCATGG | 1 cut(s) | 561 |
| NdeII | GATC | 4 cut(s) | 370, 411, 439, 709 |
| NlaIII | CATG | 3 cut(s) | 446, 565, 709 |
| NlaIV | GGNNCC | 1 cut(s) | 413 |
| NmuCI | GTSAC | 3 cut(s) | 213, 328, 767 |
| PdmI | GAANNNNTTC | 1 cut(s) | 630 |
| PkrI | GCNGC | 2 cut(s) | 170, 704 |
| PleI | GAGTC | 1 cut(s) | 335 |
| PmaCI | CACGTG | 1 cut(s) | 772 |
| PmlI | CACGTG | 1 cut(s) | 772 |
| PpsI | GAGTC | 1 cut(s) | 335 |
| Ppu21I | YACGTR | 1 cut(s) | 772 |
| PpuMI | RGGWCCY | 1 cut(s) | 274 |
| PshBI | ATTAAT | 1 cut(s) | 405 |
| Psp5II | RGGWCCY | 1 cut(s) | 274 |
| PspCI | CACGTG | 1 cut(s) | 772 |
| PspN4I | GGNNCC | 1 cut(s) | 413 |
| PspPI | GGNCC | 2 cut(s) | 75, 274 |
| PspPPI | RGGWCCY | 1 cut(s) | 274 |
| PstI | CTGCAG | 1 cut(s) | 225 |
| PsuI | RGATCY | 3 cut(s) | 370, 411, 709 |
| RsaI | GTAC | 3 cut(s) | 423, 428, 666 |
| RsaNI | GTAC | 3 cut(s) | 422, 427, 665 |
| SaqAI | TTAA | 5 cut(s) | 96, 141, 405, 528, 781 |
| SatI | GCNGC | 2 cut(s) | 169, 703 |
| Sau3AI | GATC | 4 cut(s) | 370, 411, 439, 709 |
| Sau96I | GGNCC | 2 cut(s) | 75, 274 |
| ScaI | AGTACT | 1 cut(s) | 428 |
| SchI | GAGTC | 1 cut(s) | 336 |
| SetI | ASST | 9 cut(s) | 11, 59, 141, 147, 181, 276, 335, 358, 774 |
| SfaNI | GCATC | 3 cut(s) | 333, 697, 715 |
| SfcI | CTRYAG | 2 cut(s) | 221, 243 |
| SinI | GGWCC | 2 cut(s) | 75, 274 |
| SmlI | CTYRAG | 1 cut(s) | 140 |
| SmoI | CTYRAG | 1 cut(s) | 140 |
| Sse9I | AATT | 6 cut(s) | 40, 303, 402, 432, 525, 642 |
| SspI | AATATT | 1 cut(s) | 607 |
| SspMI | CTAG | 1 cut(s) | 347 |
| StyI | CCWWGG | 2 cut(s) | 415, 561 |
| TaaI | ACNGT | 3 cut(s) | 244, 426, 669 |
| TaiI | ACGT | 1 cut(s) | 774 |
| TaqI | TCGA | 1 cut(s) | 696 |
| TasI | AATT | 6 cut(s) | 40, 303, 402, 432, 525, 642 |
| TatI | WGTACW | 2 cut(s) | 426, 664 |
| Tru1I | TTAA | 5 cut(s) | 96, 141, 405, 528, 781 |
| Tru9I | TTAA | 5 cut(s) | 96, 141, 405, 528, 781 |
| TscAI | CASTG | 1 cut(s) | 628 |
| TseFI | GTSAC | 3 cut(s) | 213, 328, 767 |
| TseI | GCWGC | 2 cut(s) | 168, 702 |
| Tsp45I | GTSAC | 3 cut(s) | 213, 328, 767 |
| TspDTI | ATGAA | 2 cut(s) | 91, 535 |
| TspRI | CASTG | 1 cut(s) | 628 |
| Vha464I | CTTAAG | 1 cut(s) | 140 |
| VpaK11BI | GGWCC | 2 cut(s) | 75, 274 |
| VspI | ATTAAT | 1 cut(s) | 405 |
| XapI | RAATTY | 1 cut(s) | 40 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 568 |
| XmiI | GTMKAC | 1 cut(s) | 466 |
| XmnI | GAANNNNTTC | 1 cut(s) | 630 |
| XspI | CTAG | 1 cut(s) | 347 |
| ZrmI | AGTACT | 1 cut(s) | 428 |