Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 262 |
| Acc36I | ACCTGC | 1 cut(s) | 262 |
| AccI | GTMKAC | 2 cut(s) | 239, 466 |
| AccIII | TCCGGA | 1 cut(s) | 499 |
| AciI | CCGC | 1 cut(s) | 409 |
| AclWI | GGATC | 3 cut(s) | 365, 406, 419 |
| AcsI | RAATTY | 2 cut(s) | 40, 450 |
| AcuI | CTGAAG | 1 cut(s) | 291 |
| AdeI | CACNNNGTG | 1 cut(s) | 182 |
| AfaI | GTAC | 1 cut(s) | 423 |
| AflII | CTTAAG | 2 cut(s) | 116, 140 |
| AgsI | TTSAA | 2 cut(s) | 149, 368 |
| AluBI | AGCT | 2 cut(s) | 92, 145 |
| AluI | AGCT | 2 cut(s) | 92, 145 |
| Alw26I | GTCTC | 2 cut(s) | 60, 129 |
| AlwI | GGATC | 3 cut(s) | 365, 406, 419 |
| Aor13HI | TCCGGA | 1 cut(s) | 499 |
| AoxI | GGCC | 1 cut(s) | 112 |
| ApeKI | GCWGC | 1 cut(s) | 173 |
| ApoI | RAATTY | 2 cut(s) | 40, 450 |
| AspS9I | GGNCC | 1 cut(s) | 379 |
| AsuC2I | CCSGG | 1 cut(s) | 437 |
| AvaII | GGWCC | 1 cut(s) | 379 |
| AxyI | CCTNAGG | 1 cut(s) | 375 |
| BamHI | GGATCC | 1 cut(s) | 411 |
| BauI | CACGAG | 1 cut(s) | 180 |
| BbvI | GCAGC | 1 cut(s) | 185 |
| BccI | CCATC | 1 cut(s) | 290 |
| BciVI | GTATCC | 1 cut(s) | 197 |
| BcnI | CCSGG | 1 cut(s) | 437 |
| BcoDI | GTCTC | 2 cut(s) | 60, 129 |
| BfrI | CTTAAG | 2 cut(s) | 116, 140 |
| BfuAI | ACCTGC | 1 cut(s) | 262 |
| BfuI | GTATCC | 1 cut(s) | 197 |
| BisI | GCNGC | 1 cut(s) | 174 |
| BlsI | GCNGC | 1 cut(s) | 175 |
| Bme1390I | CCNGG | 1 cut(s) | 437 |
| Bme18I | GGWCC | 1 cut(s) | 379 |
| BmgT120I | GGNCC | 1 cut(s) | 379 |
| BmiI | GGNNCC | 2 cut(s) | 381, 413 |
| BmrFI | CCNGG | 1 cut(s) | 437 |
| BpuMI | CCSGG | 1 cut(s) | 437 |
| BsaI | GGTCTC | 1 cut(s) | 129 |
| BsaWI | WCCGGW | 1 cut(s) | 499 |
| Bse21I | CCTNAGG | 1 cut(s) | 375 |
| BseAI | TCCGGA | 1 cut(s) | 499 |
| BseMII | CTCAG | 1 cut(s) | 212 |
| BseXI | GCAGC | 1 cut(s) | 185 |
| BsgI | GTGCAG | 1 cut(s) | 192 |
| BshFI | GGCC | 1 cut(s) | 114 |
| BsiSI | CCGG | 2 cut(s) | 437, 500 |
| BsmAI | GTCTC | 2 cut(s) | 60, 129 |
| BsnI | GGCC | 1 cut(s) | 114 |
| Bso31I | GGTCTC | 1 cut(s) | 129 |
| Bsp13I | TCCGGA | 1 cut(s) | 499 |
| Bsp143I | GATC | 2 cut(s) | 370, 411 |
| BspACI | CCGC | 1 cut(s) | 409 |
| BspANI | GGCC | 1 cut(s) | 114 |
| BspCNI | CTCAG | 1 cut(s) | 213 |
| BspEI | TCCGGA | 1 cut(s) | 499 |
| BspLI | GGNNCC | 2 cut(s) | 381, 413 |
| BspMI | ACCTGC | 1 cut(s) | 262 |
| BspPI | GGATC | 3 cut(s) | 365, 406, 419 |
| BspTI | CTTAAG | 2 cut(s) | 116, 140 |
| BspTNI | GGTCTC | 1 cut(s) | 129 |
| BssMI | GATC | 2 cut(s) | 370, 411 |
| BssSI | CACGAG | 1 cut(s) | 180 |
| Bst2BI | CACGAG | 1 cut(s) | 180 |
| Bst4CI | ACNGT | 4 cut(s) | 170, 244, 421, 443 |
| BstAFI | CTTAAG | 2 cut(s) | 116, 140 |
| BstDEI | CTNAG | 5 cut(s) | 88, 160, 221, 375, 480 |
| BstKTI | GATC | 2 cut(s) | 373, 414 |
| BstMAI | GTCTC | 2 cut(s) | 60, 129 |
| BstMBI | GATC | 2 cut(s) | 370, 411 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 263 |
| BstSCI | CCNGG | 1 cut(s) | 435 |
| BstV1I | GCAGC | 1 cut(s) | 185 |
| BstX2I | RGATCY | 2 cut(s) | 370, 411 |
| BstYI | RGATCY | 2 cut(s) | 370, 411 |
| Bsu36I | CCTNAGG | 1 cut(s) | 375 |
| BsuI | GTATCC | 1 cut(s) | 197 |
| BsuRI | GGCC | 1 cut(s) | 114 |
| BtsIMutI | CAGTG | 2 cut(s) | 175, 448 |
| BveI | ACCTGC | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 379 |
| Csp6I | GTAC | 1 cut(s) | 422 |
| CviAII | CATG | 2 cut(s) | 343, 513 |
| CviJI | RGCY | 5 cut(s) | 58, 92, 114, 145, 517 |
| CviKI_1 | RGCY | 5 cut(s) | 58, 92, 114, 145, 517 |
| CviQI | GTAC | 1 cut(s) | 422 |
| DdeI | CTNAG | 5 cut(s) | 88, 160, 221, 375, 480 |
| DpnI | GATC | 2 cut(s) | 372, 413 |
| DpnII | GATC | 2 cut(s) | 370, 411 |
| DraIII | CACNNNGTG | 1 cut(s) | 182 |
| EciI | GGCGGA | 1 cut(s) | 424 |
| Eco147I | AGGCCT | 1 cut(s) | 114 |
| Eco31I | GGTCTC | 1 cut(s) | 129 |
| Eco47I | GGWCC | 1 cut(s) | 379 |
| Eco57I | CTGAAG | 1 cut(s) | 291 |
| Eco81I | CCTNAGG | 1 cut(s) | 375 |
| EcoO109I | RGGNCCY | 1 cut(s) | 379 |
| FaeI | CATG | 2 cut(s) | 346, 516 |
| FatI | CATG | 2 cut(s) | 342, 512 |
| FblI | GTMKAC | 2 cut(s) | 239, 466 |
| Fnu4HI | GCNGC | 1 cut(s) | 174 |
| Fsp4HI | GCNGC | 1 cut(s) | 174 |
| GluI | GCNGC | 1 cut(s) | 174 |
| HaeIII | GGCC | 1 cut(s) | 114 |
| HapII | CCGG | 2 cut(s) | 437, 500 |
| Hin1II | CATG | 2 cut(s) | 346, 516 |
| HindIII | AAGCTT | 1 cut(s) | 143 |
| HinfI | GANTC | 1 cut(s) | 327 |
| HpaII | CCGG | 2 cut(s) | 437, 500 |
| Hpy166II | GTNNAC | 2 cut(s) | 240, 467 |
| Hpy188I | TCNGA | 2 cut(s) | 65, 353 |
| Hpy188III | TCNNGA | 1 cut(s) | 500 |
| Hpy8I | GTNNAC | 2 cut(s) | 240, 467 |
| Hpy99I | CGWCG | 1 cut(s) | 353 |
| HpyCH4III | ACNGT | 4 cut(s) | 170, 244, 421, 443 |
| HpyCH4V | TGCA | 4 cut(s) | 173, 266, 308, 494 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 263 |
| HpyF3I | CTNAG | 5 cut(s) | 88, 160, 221, 375, 480 |
| Hsp92II | CATG | 2 cut(s) | 346, 516 |
| Kpn2I | TCCGGA | 1 cut(s) | 499 |
| Kzo9I | GATC | 2 cut(s) | 370, 411 |
| LmnI | GCTCC | 1 cut(s) | 353 |
| LpnPI | CCDG | 6 cut(s) | 21, 44, 267, 282, 450, 513 |
| Lsp1109I | GCAGC | 1 cut(s) | 185 |
| MaeIII | GTNAC | 2 cut(s) | 328, 508 |
| MalI | GATC | 2 cut(s) | 372, 413 |
| MboI | GATC | 2 cut(s) | 370, 411 |
| MboII | GAAGA | 1 cut(s) | 380 |
| MflI | RGATCY | 2 cut(s) | 370, 411 |
| MluCI | AATT | 3 cut(s) | 40, 402, 450 |
| MlyI | GAGTC | 1 cut(s) | 336 |
| MmeI | TCCRAC | 1 cut(s) | 331 |
| MnlI | CCTC | 3 cut(s) | 7, 104, 189 |
| MroI | TCCGGA | 1 cut(s) | 499 |
| MseI | TTAA | 3 cut(s) | 96, 117, 141 |
| MslI | CAYNNNNRTG | 1 cut(s) | 526 |
| MspCI | CTTAAG | 2 cut(s) | 116, 140 |
| MspI | CCGG | 2 cut(s) | 437, 500 |
| MspR9I | CCNGG | 1 cut(s) | 437 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 263 |
| NciI | CCSGG | 1 cut(s) | 437 |
| NdeII | GATC | 2 cut(s) | 370, 411 |
| NlaIII | CATG | 2 cut(s) | 346, 516 |
| NlaIV | GGNNCC | 2 cut(s) | 381, 413 |
| NmuCI | GTSAC | 1 cut(s) | 328 |
| PaqCI | CACCTGC | 1 cut(s) | 262 |
| PceI | AGGCCT | 1 cut(s) | 114 |
| PfoI | TCCNGGA | 1 cut(s) | 435 |
| PkrI | GCNGC | 1 cut(s) | 175 |
| PleI | GAGTC | 1 cut(s) | 335 |
| PpsI | GAGTC | 1 cut(s) | 335 |
| PpuMI | RGGWCCY | 1 cut(s) | 379 |
| Psp5II | RGGWCCY | 1 cut(s) | 379 |
| PspN4I | GGNNCC | 2 cut(s) | 381, 413 |
| PspPI | GGNCC | 1 cut(s) | 379 |
| PspPPI | RGGWCCY | 1 cut(s) | 379 |
| PsuI | RGATCY | 2 cut(s) | 370, 411 |
| RsaI | GTAC | 1 cut(s) | 423 |
| RsaNI | GTAC | 1 cut(s) | 422 |
| RseI | CAYNNNNRTG | 1 cut(s) | 526 |
| SaqAI | TTAA | 3 cut(s) | 96, 117, 141 |
| SatI | GCNGC | 1 cut(s) | 174 |
| Sau3AI | GATC | 2 cut(s) | 370, 411 |
| Sau96I | GGNCC | 1 cut(s) | 379 |
| SchI | GAGTC | 1 cut(s) | 336 |
| ScrFI | CCNGG | 1 cut(s) | 437 |
| SetI | ASST | 9 cut(s) | 18, 94, 141, 147, 181, 256, 271, 474, 510 |
| SinI | GGWCC | 1 cut(s) | 379 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 526 |
| SmlI | CTYRAG | 2 cut(s) | 116, 140 |
| SmoI | CTYRAG | 2 cut(s) | 116, 140 |
| Sse9I | AATT | 3 cut(s) | 40, 402, 450 |
| SseBI | AGGCCT | 1 cut(s) | 114 |
| SsiI | CCGC | 1 cut(s) | 409 |
| StuI | AGGCCT | 1 cut(s) | 114 |
| StyD4I | CCNGG | 1 cut(s) | 435 |
| TaaI | ACNGT | 4 cut(s) | 170, 244, 421, 443 |
| TaqI | TCGA | 1 cut(s) | 209 |
| TasI | AATT | 3 cut(s) | 40, 402, 450 |
| Tru1I | TTAA | 3 cut(s) | 96, 117, 141 |
| Tru9I | TTAA | 3 cut(s) | 96, 117, 141 |
| TscAI | CASTG | 2 cut(s) | 175, 448 |
| TseFI | GTSAC | 1 cut(s) | 328 |
| TseI | GCWGC | 1 cut(s) | 173 |
| Tsp45I | GTSAC | 1 cut(s) | 328 |
| TspDTI | ATGAA | 3 cut(s) | 91, 401, 510 |
| TspRI | CASTG | 2 cut(s) | 175, 448 |
| Vha464I | CTTAAG | 2 cut(s) | 116, 140 |
| VpaK11BI | GGWCC | 1 cut(s) | 379 |
| XapI | RAATTY | 2 cut(s) | 40, 450 |
| XmiI | GTMKAC | 2 cut(s) | 239, 466 |