Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 697 |
| AciI | CCGC | 4 cut(s) | 47, 78, 399, 830 |
| AclWI | GGATC | 2 cut(s) | 153, 596 |
| AcsI | RAATTY | 6 cut(s) | 40, 185, 264, 281, 382, 642 |
| AcuI | CTGAAG | 1 cut(s) | 522 |
| AdeI | CACNNNGTG | 1 cut(s) | 413 |
| AfaI | GTAC | 2 cut(s) | 338, 654 |
| AflII | CTTAAG | 1 cut(s) | 371 |
| AgsI | TTSAA | 7 cut(s) | 137, 166, 185, 380, 455, 599, 857 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 942, 974 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 143, 175, 827, 859 |
| AluBI | AGCT | 3 cut(s) | 35, 376, 953 |
| AluI | AGCT | 3 cut(s) | 35, 376, 953 |
| AlwI | GGATC | 2 cut(s) | 153, 596 |
| ApeKI | GCWGC | 1 cut(s) | 239 |
| ApoI | RAATTY | 6 cut(s) | 40, 185, 264, 281, 382, 642 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 132, 535, 861 |
| AspLEI | GCGC | 1 cut(s) | 993 |
| AspS9I | GGNCC | 4 cut(s) | 196, 610, 901, 965 |
| AsuC2I | CCSGG | 1 cut(s) | 688 |
| AsuHPI | GGTGA | 4 cut(s) | 61, 553, 844, 992 |
| AvaII | GGWCC | 4 cut(s) | 196, 610, 901, 965 |
| AxyI | CCTNAGG | 1 cut(s) | 606 |
| BauI | CACGAG | 1 cut(s) | 411 |
| BbsI | GAAGAC | 1 cut(s) | 895 |
| BbvI | GCAGC | 1 cut(s) | 226 |
| BccI | CCATC | 5 cut(s) | 389, 521, 774, 796, 850 |
| BciVI | GTATCC | 1 cut(s) | 428 |
| BcnI | CCSGG | 1 cut(s) | 688 |
| BfaI | CTAG | 2 cut(s) | 218, 513 |
| BfmI | CTRYAG | 1 cut(s) | 240 |
| BfrI | CTTAAG | 1 cut(s) | 371 |
| BfuI | GTATCC | 1 cut(s) | 428 |
| BglII | AGATCT | 1 cut(s) | 367 |
| BisI | GCNGC | 2 cut(s) | 240, 400 |
| BlsI | GCNGC | 2 cut(s) | 241, 401 |
| Bme1390I | CCNGG | 1 cut(s) | 688 |
| Bme18I | GGWCC | 4 cut(s) | 196, 610, 901, 965 |
| BmgT120I | GGNCC | 4 cut(s) | 196, 610, 901, 965 |
| BmiI | GGNNCC | 2 cut(s) | 612, 903 |
| BmrFI | CCNGG | 1 cut(s) | 688 |
| BmsI | GCATC | 1 cut(s) | 928 |
| BpiI | GAAGAC | 1 cut(s) | 895 |
| BpuMI | CCSGG | 1 cut(s) | 688 |
| BsaAI | YACGTR | 1 cut(s) | 336 |
| BsaJI | CCNNGG | 2 cut(s) | 687, 770 |
| Bse1I | ACTGG | 1 cut(s) | 931 |
| Bse21I | CCTNAGG | 1 cut(s) | 606 |
| Bse3DI | GCAATG | 1 cut(s) | 832 |
| BseDI | CCNNGG | 2 cut(s) | 687, 770 |
| BseGI | GGATG | 2 cut(s) | 766, 842 |
| BseMI | GCAATG | 1 cut(s) | 832 |
| BseMII | CTCAG | 2 cut(s) | 473, 620 |
| BseNI | ACTGG | 1 cut(s) | 931 |
| BseRI | GAGGAG | 1 cut(s) | 261 |
| BseXI | GCAGC | 1 cut(s) | 226 |
| BseYI | CCCAGC | 1 cut(s) | 35 |
| BsiSI | CCGG | 1 cut(s) | 687 |
| BslFI | GGGAC | 1 cut(s) | 887 |
| BsmFI | GGGAC | 1 cut(s) | 887 |
| Bsp1407I | TGTACA | 1 cut(s) | 652 |
| Bsp143I | GATC | 4 cut(s) | 158, 367, 576, 601 |
| BspACI | CCGC | 4 cut(s) | 47, 78, 399, 830 |
| BspCNI | CTCAG | 2 cut(s) | 472, 619 |
| BspHI | TCATGA | 2 cut(s) | 147, 573 |
| BspLI | GGNNCC | 2 cut(s) | 612, 903 |
| BspMAI | CTGCAG | 1 cut(s) | 244 |
| BspPI | GGATC | 2 cut(s) | 153, 596 |
| BspTI | CTTAAG | 1 cut(s) | 371 |
| BsrDI | GCAATG | 1 cut(s) | 832 |
| BsrGI | TGTACA | 1 cut(s) | 652 |
| BsrI | ACTGG | 1 cut(s) | 931 |
| BssECI | CCNNGG | 2 cut(s) | 687, 770 |
| BssMI | GATC | 4 cut(s) | 158, 367, 576, 601 |
| BssSI | CACGAG | 1 cut(s) | 411 |
| BssT1I | CCWWGG | 1 cut(s) | 770 |
| Bst2BI | CACGAG | 1 cut(s) | 411 |
| Bst4CI | ACNGT | 3 cut(s) | 255, 341, 910 |
| BstAFI | CTTAAG | 1 cut(s) | 371 |
| BstAUI | TGTACA | 1 cut(s) | 652 |
| BstBAI | YACGTR | 1 cut(s) | 336 |
| BstDEI | CTNAG | 4 cut(s) | 391, 459, 606, 647 |
| BstF5I | GGATG | 2 cut(s) | 766, 842 |
| BstHHI | GCGC | 1 cut(s) | 993 |
| BstKTI | GATC | 4 cut(s) | 161, 370, 579, 604 |
| BstMBI | GATC | 4 cut(s) | 158, 367, 576, 601 |
| BstSCI | CCNGG | 1 cut(s) | 686 |
| BstSFI | CTRYAG | 1 cut(s) | 240 |
| BstSNI | TACGTA | 1 cut(s) | 336 |
| BstV1I | GCAGC | 1 cut(s) | 226 |
| BstV2I | GAAGAC | 1 cut(s) | 895 |
| BstX2I | RGATCY | 3 cut(s) | 158, 367, 601 |
| BstYI | RGATCY | 3 cut(s) | 158, 367, 601 |
| Bsu36I | CCTNAGG | 1 cut(s) | 606 |
| BsuI | GTATCC | 1 cut(s) | 428 |
| BtsCI | GGATG | 2 cut(s) | 766, 842 |
| BtsIMutI | CAGTG | 1 cut(s) | 924 |
| CciI | TCATGA | 2 cut(s) | 147, 573 |
| CfoI | GCGC | 1 cut(s) | 993 |
| Cfr13I | GGNCC | 4 cut(s) | 196, 610, 901, 965 |
| Csp6I | GTAC | 2 cut(s) | 337, 653 |
| CviAII | CATG | 3 cut(s) | 148, 574, 937 |
| CviJI | RGCY | 7 cut(s) | 35, 275, 376, 463, 765, 941, 953 |
| CviKI_1 | RGCY | 7 cut(s) | 35, 275, 376, 463, 765, 941, 953 |
| CviQI | GTAC | 2 cut(s) | 337, 653 |
| DdeI | CTNAG | 4 cut(s) | 391, 459, 606, 647 |
| DpnI | GATC | 4 cut(s) | 160, 369, 578, 603 |
| DpnII | GATC | 4 cut(s) | 158, 367, 576, 601 |
| DraI | TTTAAA | 3 cut(s) | 190, 618, 897 |
| DraIII | CACNNNGTG | 1 cut(s) | 413 |
| Eco105I | TACGTA | 1 cut(s) | 336 |
| Eco130I | CCWWGG | 1 cut(s) | 770 |
| Eco32I | GATATC | 1 cut(s) | 324 |
| Eco47I | GGWCC | 4 cut(s) | 196, 610, 901, 965 |
| Eco57I | CTGAAG | 1 cut(s) | 522 |
| Eco81I | CCTNAGG | 1 cut(s) | 606 |
| EcoO109I | RGGNCCY | 3 cut(s) | 196, 610, 901 |
| EcoRI | GAATTC | 1 cut(s) | 642 |
| EcoRV | GATATC | 1 cut(s) | 324 |
| EcoT14I | CCWWGG | 1 cut(s) | 770 |
| EcoT22I | ATGCAT | 1 cut(s) | 354 |
| ErhI | CCWWGG | 1 cut(s) | 770 |
| FaeI | CATG | 3 cut(s) | 151, 577, 940 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 877, 909 |
| FaqI | GGGAC | 1 cut(s) | 887 |
| FatI | CATG | 3 cut(s) | 147, 573, 936 |
| FblI | GTMKAC | 1 cut(s) | 697 |
| Fnu4HI | GCNGC | 2 cut(s) | 240, 400 |
| FokI | GGATG | 2 cut(s) | 753, 829 |
| Fsp4HI | GCNGC | 2 cut(s) | 240, 400 |
| FspBI | CTAG | 2 cut(s) | 218, 513 |
| GlaI | GCGC | 1 cut(s) | 992 |
| GluI | GCNGC | 2 cut(s) | 240, 400 |
| GsaI | CCCAGC | 1 cut(s) | 39 |
| HapII | CCGG | 1 cut(s) | 687 |
| HhaI | GCGC | 1 cut(s) | 993 |
| Hin1II | CATG | 3 cut(s) | 151, 577, 940 |
| Hin6I | GCGC | 1 cut(s) | 991 |
| HinP1I | GCGC | 1 cut(s) | 991 |
| HindIII | AAGCTT | 1 cut(s) | 374 |
| HinfI | GANTC | 1 cut(s) | 558 |
| HpaII | CCGG | 1 cut(s) | 687 |
| HphI | GGTGA | 4 cut(s) | 61, 553, 844, 992 |
| Hpy166II | GTNNAC | 4 cut(s) | 653, 655, 698, 835 |
| Hpy188I | TCNGA | 4 cut(s) | 65, 434, 541, 680 |
| Hpy188III | TCNNGA | 7 cut(s) | 148, 327, 513, 574, 608, 944, 968 |
| Hpy8I | GTNNAC | 4 cut(s) | 653, 655, 698, 835 |
| HpyAV | CCTTC | 1 cut(s) | 800 |
| HpyCH4III | ACNGT | 3 cut(s) | 255, 341, 910 |
| HpyCH4IV | ACGT | 1 cut(s) | 335 |
| HpyCH4V | TGCA | 5 cut(s) | 22, 242, 270, 352, 497 |
| HpyF3I | CTNAG | 4 cut(s) | 391, 459, 606, 647 |
| HpySE526I | ACGT | 1 cut(s) | 335 |
| Hsp92II | CATG | 3 cut(s) | 151, 577, 940 |
| HspAI | GCGC | 1 cut(s) | 991 |
| Kzo9I | GATC | 4 cut(s) | 158, 367, 576, 601 |
| LmnI | GCTCC | 1 cut(s) | 32 |
| Lsp1109I | GCAGC | 1 cut(s) | 226 |
| LweI | GCATC | 1 cut(s) | 928 |
| MaeI | CTAG | 2 cut(s) | 218, 513 |
| MaeII | ACGT | 1 cut(s) | 335 |
| MaeIII | GTNAC | 3 cut(s) | 49, 444, 559 |
| MalI | GATC | 4 cut(s) | 160, 369, 578, 603 |
| MboI | GATC | 4 cut(s) | 158, 367, 576, 601 |
| MboII | GAAGA | 6 cut(s) | 130, 301, 571, 611, 869, 900 |
| MfeI | CAATTG | 1 cut(s) | 317 |
| MflI | RGATCY | 3 cut(s) | 158, 367, 601 |
| MlyI | GAGTC | 1 cut(s) | 567 |
| MmeI | TCCRAC | 1 cut(s) | 940 |
| MnlI | CCTC | 8 cut(s) | 104, 187, 239, 289, 420, 615, 803, 956 |
| Mph1103I | ATGCAT | 1 cut(s) | 354 |
| MroXI | GAANNNNTTC | 3 cut(s) | 132, 535, 861 |
| MseI | TTAA | 6 cut(s) | 96, 189, 372, 617, 759, 896 |
| MspCI | CTTAAG | 1 cut(s) | 371 |
| MspI | CCGG | 1 cut(s) | 687 |
| MspR9I | CCNGG | 1 cut(s) | 688 |
| MunI | CAATTG | 1 cut(s) | 317 |
| NciI | CCSGG | 1 cut(s) | 688 |
| NdeII | GATC | 4 cut(s) | 158, 367, 576, 601 |
| NlaIII | CATG | 3 cut(s) | 151, 577, 940 |
| NlaIV | GGNNCC | 2 cut(s) | 612, 903 |
| NmuCI | GTSAC | 3 cut(s) | 49, 444, 559 |
| NsiI | ATGCAT | 1 cut(s) | 354 |
| PagI | TCATGA | 2 cut(s) | 147, 573 |
| PdmI | GAANNNNTTC | 3 cut(s) | 132, 535, 861 |
| PkrI | GCNGC | 2 cut(s) | 241, 401 |
| PleI | GAGTC | 1 cut(s) | 566 |
| PpsI | GAGTC | 1 cut(s) | 566 |
| Ppu21I | YACGTR | 1 cut(s) | 336 |
| PpuMI | RGGWCCY | 3 cut(s) | 196, 610, 901 |
| Psp5II | RGGWCCY | 3 cut(s) | 196, 610, 901 |
| PspFI | CCCAGC | 1 cut(s) | 35 |
| PspN4I | GGNNCC | 2 cut(s) | 612, 903 |
| PspPI | GGNCC | 4 cut(s) | 196, 610, 901, 965 |
| PspPPI | RGGWCCY | 3 cut(s) | 196, 610, 901 |
| PstI | CTGCAG | 1 cut(s) | 244 |
| PsuI | RGATCY | 3 cut(s) | 158, 367, 601 |
| RsaI | GTAC | 2 cut(s) | 338, 654 |
| RsaNI | GTAC | 2 cut(s) | 337, 653 |
| SaqAI | TTAA | 6 cut(s) | 96, 189, 372, 617, 759, 896 |
| SatI | GCNGC | 2 cut(s) | 240, 400 |
| Sau3AI | GATC | 4 cut(s) | 158, 367, 576, 601 |
| Sau96I | GGNCC | 4 cut(s) | 196, 610, 901, 965 |
| SchI | GAGTC | 1 cut(s) | 567 |
| ScrFI | CCNGG | 1 cut(s) | 688 |
| SfaNI | GCATC | 1 cut(s) | 928 |
| SfcI | CTRYAG | 1 cut(s) | 240 |
| SinI | GGWCC | 4 cut(s) | 196, 610, 901, 965 |
| SmlI | CTYRAG | 1 cut(s) | 371 |
| SmoI | CTYRAG | 1 cut(s) | 371 |
| SnaBI | TACGTA | 1 cut(s) | 336 |
| SsiI | CCGC | 4 cut(s) | 47, 78, 399, 830 |
| SspI | AATATT | 2 cut(s) | 178, 717 |
| SspMI | CTAG | 2 cut(s) | 218, 513 |
| StyD4I | CCNGG | 1 cut(s) | 686 |
| StyI | CCWWGG | 1 cut(s) | 770 |
| TaaI | ACNGT | 3 cut(s) | 255, 341, 910 |
| TaiI | ACGT | 1 cut(s) | 338 |
| TatI | WGTACW | 1 cut(s) | 652 |
| TauI | GCSGC | 1 cut(s) | 402 |
| Tru1I | TTAA | 6 cut(s) | 96, 189, 372, 617, 759, 896 |
| Tru9I | TTAA | 6 cut(s) | 96, 189, 372, 617, 759, 896 |
| TscAI | CASTG | 1 cut(s) | 931 |
| TseFI | GTSAC | 3 cut(s) | 49, 444, 559 |
| TseI | GCWGC | 1 cut(s) | 239 |
| Tsp45I | GTSAC | 3 cut(s) | 49, 444, 559 |
| TspDTI | ATGAA | 3 cut(s) | 141, 164, 963 |
| TspRI | CASTG | 1 cut(s) | 931 |
| Vha464I | CTTAAG | 1 cut(s) | 371 |
| VpaK11BI | GGWCC | 4 cut(s) | 196, 610, 901, 965 |
| XapI | RAATTY | 6 cut(s) | 40, 185, 264, 281, 382, 642 |
| XbaI | TCTAGA | 1 cut(s) | 512 |
| XmiI | GTMKAC | 1 cut(s) | 697 |
| XmnI | GAANNNNTTC | 3 cut(s) | 132, 535, 861 |
| XspI | CTAG | 2 cut(s) | 218, 513 |
| Zsp2I | ATGCAT | 1 cut(s) | 354 |