Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 406 |
| AciI | CCGC | 2 cut(s) | 479, 643 |
| AclWI | GGATC | 5 cut(s) | 153, 442, 599, 640, 653 |
| AfaI | GTAC | 3 cut(s) | 76, 135, 681 |
| AflII | CTTAAG | 1 cut(s) | 371 |
| AgsI | TTSAA | 4 cut(s) | 166, 185, 190, 602 |
| AjnI | CCWGG | 1 cut(s) | 670 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 143, 175 |
| AluBI | AGCT | 1 cut(s) | 376 |
| AluI | AGCT | 1 cut(s) | 376 |
| Alw21I | GWGCWC | 1 cut(s) | 87 |
| Alw26I | GTCTC | 2 cut(s) | 568, 696 |
| AlwI | GGATC | 5 cut(s) | 153, 442, 599, 640, 653 |
| Ama87I | CYCGRG | 1 cut(s) | 411 |
| AoxI | GGCC | 1 cut(s) | 903 |
| ApeKI | GCWGC | 3 cut(s) | 239, 476, 936 |
| AspS9I | GGNCC | 4 cut(s) | 196, 508, 613, 904 |
| AsuHPI | GGTGA | 1 cut(s) | 427 |
| AvaI | CYCGRG | 1 cut(s) | 411 |
| AvaII | GGWCC | 3 cut(s) | 196, 508, 613 |
| AxyI | CCTNAGG | 2 cut(s) | 504, 609 |
| BamHI | GGATCC | 1 cut(s) | 645 |
| BanI | GGYRCC | 1 cut(s) | 406 |
| Bbv12I | GWGCWC | 1 cut(s) | 87 |
| BbvI | GCAGC | 3 cut(s) | 226, 463, 948 |
| BccI | CCATC | 4 cut(s) | 223, 524, 849, 853 |
| BceAI | ACGGC | 1 cut(s) | 420 |
| BciT130I | CCWGG | 1 cut(s) | 672 |
| BcoDI | GTCTC | 2 cut(s) | 568, 696 |
| BfaI | CTAG | 1 cut(s) | 218 |
| BfrI | CTTAAG | 1 cut(s) | 371 |
| BglII | AGATCT | 1 cut(s) | 367 |
| BisI | GCNGC | 3 cut(s) | 240, 477, 937 |
| BlsI | GCNGC | 3 cut(s) | 241, 478, 938 |
| Bme1390I | CCNGG | 1 cut(s) | 672 |
| Bme18I | GGWCC | 3 cut(s) | 196, 508, 613 |
| BmeT110I | CYCGRG | 1 cut(s) | 411 |
| BmgT120I | GGNCC | 4 cut(s) | 196, 508, 613, 904 |
| BmiI | GGNNCC | 4 cut(s) | 408, 615, 647, 906 |
| BmrFI | CCNGG | 1 cut(s) | 672 |
| BmsI | GCATC | 2 cut(s) | 178, 943 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 237, 269, 693, 725 |
| BpmI | CTGGAG | 1 cut(s) | 264 |
| BsaI | GGTCTC | 1 cut(s) | 568 |
| BsaJI | CCNNGG | 2 cut(s) | 410, 670 |
| BsaWI | WCCGGW | 1 cut(s) | 77 |
| Bse1I | ACTGG | 1 cut(s) | 276 |
| Bse21I | CCTNAGG | 2 cut(s) | 504, 609 |
| Bse3DI | GCAATG | 1 cut(s) | 835 |
| BseBI | CCWGG | 1 cut(s) | 672 |
| BseDI | CCNNGG | 2 cut(s) | 410, 670 |
| BseGI | GGATG | 3 cut(s) | 169, 841, 845 |
| BseMI | GCAATG | 1 cut(s) | 835 |
| BseMII | CTCAG | 1 cut(s) | 495 |
| BseNI | ACTGG | 1 cut(s) | 276 |
| BseRI | GAGGAG | 1 cut(s) | 261 |
| BseXI | GCAGC | 3 cut(s) | 226, 463, 948 |
| BshFI | GGCC | 1 cut(s) | 905 |
| BshNI | GGYRCC | 1 cut(s) | 406 |
| BsiHKAI | GWGCWC | 1 cut(s) | 87 |
| BsiHKCI | CYCGRG | 1 cut(s) | 411 |
| BsiSI | CCGG | 2 cut(s) | 78, 488 |
| BsmAI | GTCTC | 2 cut(s) | 568, 696 |
| BsnI | GGCC | 1 cut(s) | 905 |
| Bso31I | GGTCTC | 1 cut(s) | 568 |
| BsoBI | CYCGRG | 1 cut(s) | 411 |
| Bsp1286I | GDGCHC | 1 cut(s) | 87 |
| Bsp1407I | TGTACA | 1 cut(s) | 133 |
| Bsp143I | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| BspACI | CCGC | 2 cut(s) | 479, 643 |
| BspANI | GGCC | 1 cut(s) | 905 |
| BspCNI | CTCAG | 1 cut(s) | 496 |
| BspHI | TCATGA | 2 cut(s) | 147, 576 |
| BspLI | GGNNCC | 4 cut(s) | 408, 615, 647, 906 |
| BspPI | GGATC | 5 cut(s) | 153, 442, 599, 640, 653 |
| BspT107I | GGYRCC | 1 cut(s) | 406 |
| BspTI | CTTAAG | 1 cut(s) | 371 |
| BspTNI | GGTCTC | 1 cut(s) | 568 |
| BsrDI | GCAATG | 1 cut(s) | 835 |
| BsrGI | TGTACA | 1 cut(s) | 133 |
| BsrI | ACTGG | 1 cut(s) | 276 |
| BssECI | CCNNGG | 2 cut(s) | 410, 670 |
| BssMI | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| Bst2UI | CCWGG | 1 cut(s) | 672 |
| Bst4CI | ACNGT | 3 cut(s) | 655, 773, 913 |
| BstAFI | CTTAAG | 1 cut(s) | 371 |
| BstAUI | TGTACA | 1 cut(s) | 133 |
| BstC8I | GCNNGC | 1 cut(s) | 711 |
| BstDEI | CTNAG | 5 cut(s) | 391, 504, 609, 626, 714 |
| BstF5I | GGATG | 3 cut(s) | 169, 841, 845 |
| BstKTI | GATC | 6 cut(s) | 123, 161, 370, 437, 607, 648 |
| BstMAI | GTCTC | 2 cut(s) | 568, 696 |
| BstMBI | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 497 |
| BstNI | CCWGG | 1 cut(s) | 672 |
| BstSCI | CCNGG | 1 cut(s) | 670 |
| BstV1I | GCAGC | 3 cut(s) | 226, 463, 948 |
| BstX2I | RGATCY | 4 cut(s) | 158, 367, 604, 645 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 229 |
| BstYI | RGATCY | 4 cut(s) | 158, 367, 604, 645 |
| Bsu36I | CCTNAGG | 2 cut(s) | 504, 609 |
| BsuRI | GGCC | 1 cut(s) | 905 |
| BtsCI | GGATG | 3 cut(s) | 169, 841, 845 |
| BtsIMutI | CAGTG | 1 cut(s) | 769 |
| Cac8I | GCNNGC | 1 cut(s) | 711 |
| CciI | TCATGA | 2 cut(s) | 147, 576 |
| Cfr13I | GGNCC | 4 cut(s) | 196, 508, 613, 904 |
| Csp6I | GTAC | 3 cut(s) | 75, 134, 680 |
| CviAII | CATG | 3 cut(s) | 148, 577, 940 |
| CviJI | RGCY | 7 cut(s) | 41, 221, 275, 376, 491, 905, 944 |
| CviKI_1 | RGCY | 7 cut(s) | 41, 221, 275, 376, 491, 905, 944 |
| CviQI | GTAC | 3 cut(s) | 75, 134, 680 |
| DdeI | CTNAG | 5 cut(s) | 391, 504, 609, 626, 714 |
| DpnI | GATC | 6 cut(s) | 122, 160, 369, 436, 606, 647 |
| DpnII | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| DraI | TTTAAA | 1 cut(s) | 900 |
| Eco31I | GGTCTC | 1 cut(s) | 568 |
| Eco32I | GATATC | 1 cut(s) | 324 |
| Eco47I | GGWCC | 3 cut(s) | 196, 508, 613 |
| Eco81I | CCTNAGG | 2 cut(s) | 504, 609 |
| Eco88I | CYCGRG | 1 cut(s) | 411 |
| EcoO109I | RGGNCCY | 4 cut(s) | 196, 508, 613, 904 |
| EcoRII | CCWGG | 1 cut(s) | 670 |
| EcoRV | GATATC | 1 cut(s) | 324 |
| FaeI | CATG | 3 cut(s) | 151, 580, 943 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 880, 912 |
| FatI | CATG | 3 cut(s) | 147, 576, 939 |
| FauI | CCCGC | 1 cut(s) | 472 |
| Fnu4HI | GCNGC | 3 cut(s) | 240, 477, 937 |
| FokI | GGATG | 3 cut(s) | 156, 828, 832 |
| Fsp4HI | GCNGC | 3 cut(s) | 240, 477, 937 |
| FspBI | CTAG | 1 cut(s) | 218 |
| GluI | GCNGC | 3 cut(s) | 240, 477, 937 |
| GsuI | CTGGAG | 1 cut(s) | 264 |
| HaeIII | GGCC | 1 cut(s) | 905 |
| HapII | CCGG | 2 cut(s) | 78, 488 |
| Hin1II | CATG | 3 cut(s) | 151, 580, 943 |
| HinfI | GANTC | 1 cut(s) | 922 |
| HpaII | CCGG | 2 cut(s) | 78, 488 |
| HphI | GGTGA | 1 cut(s) | 427 |
| Hpy166II | GTNNAC | 1 cut(s) | 838 |
| Hpy188I | TCNGA | 2 cut(s) | 517, 757 |
| Hpy188III | TCNNGA | 4 cut(s) | 106, 148, 327, 577 |
| Hpy8I | GTNNAC | 1 cut(s) | 838 |
| HpyAV | CCTTC | 1 cut(s) | 803 |
| HpyCH4III | ACNGT | 3 cut(s) | 655, 773, 913 |
| HpyCH4V | TGCA | 5 cut(s) | 270, 386, 466, 500, 542 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 497 |
| HpyF3I | CTNAG | 5 cut(s) | 391, 504, 609, 626, 714 |
| Hsp92II | CATG | 3 cut(s) | 151, 580, 943 |
| Kzo9I | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| LmnI | GCTCC | 1 cut(s) | 587 |
| Lsp1109I | GCAGC | 3 cut(s) | 226, 463, 948 |
| LweI | GCATC | 2 cut(s) | 178, 943 |
| MaeI | CTAG | 1 cut(s) | 218 |
| MaeIII | GTNAC | 2 cut(s) | 335, 444 |
| MalI | GATC | 6 cut(s) | 122, 160, 369, 436, 606, 647 |
| MboI | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| MboII | GAAGA | 3 cut(s) | 301, 614, 872 |
| MfeI | CAATTG | 1 cut(s) | 317 |
| MflI | RGATCY | 4 cut(s) | 158, 367, 604, 645 |
| MhlI | GDGCHC | 1 cut(s) | 87 |
| MluCI | AATT | 7 cut(s) | 185, 317, 636, 744, 750, 759, 973 |
| MmeI | TCCRAC | 3 cut(s) | 565, 910, 946 |
| MnlI | CCTC | 9 cut(s) | 104, 187, 239, 289, 420, 499, 806, 877, 962 |
| MseI | TTAA | 6 cut(s) | 96, 372, 762, 782, 899, 976 |
| MslI | CAYNNNNRTG | 2 cut(s) | 174, 227 |
| MspCI | CTTAAG | 1 cut(s) | 371 |
| MspI | CCGG | 2 cut(s) | 78, 488 |
| MspR9I | CCNGG | 1 cut(s) | 672 |
| MunI | CAATTG | 1 cut(s) | 317 |
| MvaI | CCWGG | 1 cut(s) | 672 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 497 |
| NdeII | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| NlaIII | CATG | 3 cut(s) | 151, 580, 943 |
| NlaIV | GGNNCC | 4 cut(s) | 408, 615, 647, 906 |
| NmuCI | GTSAC | 1 cut(s) | 444 |
| PagI | TCATGA | 2 cut(s) | 147, 576 |
| PfeI | GAWTC | 1 cut(s) | 922 |
| PkrI | GCNGC | 3 cut(s) | 241, 478, 938 |
| PpuMI | RGGWCCY | 3 cut(s) | 196, 508, 613 |
| Psp5II | RGGWCCY | 3 cut(s) | 196, 508, 613 |
| Psp6I | CCWGG | 1 cut(s) | 670 |
| PspGI | CCWGG | 1 cut(s) | 670 |
| PspN4I | GGNNCC | 4 cut(s) | 408, 615, 647, 906 |
| PspPI | GGNCC | 4 cut(s) | 196, 508, 613, 904 |
| PspPPI | RGGWCCY | 3 cut(s) | 196, 508, 613 |
| PsuI | RGATCY | 4 cut(s) | 158, 367, 604, 645 |
| RsaI | GTAC | 3 cut(s) | 76, 135, 681 |
| RsaNI | GTAC | 3 cut(s) | 75, 134, 680 |
| RseI | CAYNNNNRTG | 2 cut(s) | 174, 227 |
| SaqAI | TTAA | 6 cut(s) | 96, 372, 762, 782, 899, 976 |
| SatI | GCNGC | 3 cut(s) | 240, 477, 937 |
| Sau3AI | GATC | 6 cut(s) | 120, 158, 367, 434, 604, 645 |
| Sau96I | GGNCC | 4 cut(s) | 196, 508, 613, 904 |
| ScrFI | CCNGG | 1 cut(s) | 672 |
| SduI | GDGCHC | 1 cut(s) | 87 |
| SfaNI | GCATC | 2 cut(s) | 178, 943 |
| SinI | GGWCC | 3 cut(s) | 196, 508, 613 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 174, 227 |
| SmlI | CTYRAG | 1 cut(s) | 371 |
| SmoI | CTYRAG | 1 cut(s) | 371 |
| Sse9I | AATT | 7 cut(s) | 185, 317, 636, 744, 750, 759, 973 |
| SsiI | CCGC | 2 cut(s) | 479, 643 |
| SspMI | CTAG | 1 cut(s) | 218 |
| StyD4I | CCNGG | 1 cut(s) | 670 |
| TaaI | ACNGT | 3 cut(s) | 655, 773, 913 |
| TaqI | TCGA | 1 cut(s) | 105 |
| TasI | AATT | 7 cut(s) | 185, 317, 636, 744, 750, 759, 973 |
| TatI | WGTACW | 1 cut(s) | 133 |
| TfiI | GAWTC | 1 cut(s) | 922 |
| Tru1I | TTAA | 6 cut(s) | 96, 372, 762, 782, 899, 976 |
| Tru9I | TTAA | 6 cut(s) | 96, 372, 762, 782, 899, 976 |
| TscAI | CASTG | 1 cut(s) | 776 |
| TseFI | GTSAC | 1 cut(s) | 444 |
| TseI | GCWGC | 3 cut(s) | 239, 476, 936 |
| Tsp45I | GTSAC | 1 cut(s) | 444 |
| TspDTI | ATGAA | 4 cut(s) | 91, 141, 164, 365 |
| TspRI | CASTG | 1 cut(s) | 776 |
| Vha464I | CTTAAG | 1 cut(s) | 371 |
| VpaK11BI | GGWCC | 3 cut(s) | 196, 508, 613 |
| XspI | CTAG | 1 cut(s) | 218 |