Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 169 |
| Acc36I | ACCTGC | 1 cut(s) | 169 |
| AccI | GTMKAC | 3 cut(s) | 146, 373, 1072 |
| AciI | CCGC | 2 cut(s) | 760, 1205 |
| AclWI | GGATC | 4 cut(s) | 272, 313, 326, 971 |
| AcoI | YGGCCR | 1 cut(s) | 726 |
| AcsI | RAATTY | 5 cut(s) | 40, 357, 423, 663, 1017 |
| AcuI | CTGAAG | 1 cut(s) | 897 |
| AfaI | GTAC | 3 cut(s) | 330, 781, 1029 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 447 |
| AflII | CTTAAG | 1 cut(s) | 116 |
| AgeI | ACCGGT | 1 cut(s) | 711 |
| AgsI | TTSAA | 5 cut(s) | 275, 533, 638, 974, 1232 |
| AjnI | CCWGG | 1 cut(s) | 728 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1317, 1349 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1202, 1234 |
| AluBI | AGCT | 3 cut(s) | 92, 645, 1328 |
| AluI | AGCT | 3 cut(s) | 92, 645, 1328 |
| Alw26I | GTCTC | 1 cut(s) | 60 |
| AlwI | GGATC | 4 cut(s) | 272, 313, 326, 971 |
| AoxI | GGCC | 3 cut(s) | 112, 726, 799 |
| ApeKI | GCWGC | 1 cut(s) | 609 |
| ApoI | RAATTY | 5 cut(s) | 40, 357, 423, 663, 1017 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 698, 730 |
| AsiGI | ACCGGT | 1 cut(s) | 711 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 537, 910, 1236 |
| AspLEI | GCGC | 4 cut(s) | 703, 770, 838, 1368 |
| AspS9I | GGNCC | 6 cut(s) | 286, 577, 799, 985, 1276, 1340 |
| AsuC2I | CCSGG | 2 cut(s) | 407, 1063 |
| AsuHPI | GGTGA | 5 cut(s) | 670, 670, 928, 1219, 1367 |
| AvaII | GGWCC | 5 cut(s) | 286, 577, 985, 1276, 1340 |
| AxyI | CCTNAGG | 2 cut(s) | 282, 981 |
| BalI | TGGCCA | 1 cut(s) | 728 |
| BamHI | GGATCC | 1 cut(s) | 318 |
| BbsI | GAAGAC | 1 cut(s) | 1270 |
| BbvI | GCAGC | 1 cut(s) | 621 |
| BccI | CCATC | 7 cut(s) | 197, 526, 685, 896, 1149, 1171, 1225 |
| BciT130I | CCWGG | 1 cut(s) | 730 |
| BcnI | CCSGG | 2 cut(s) | 407, 1063 |
| BcoDI | GTCTC | 1 cut(s) | 60 |
| BfaI | CTAG | 2 cut(s) | 771, 888 |
| BfoI | RGCGCY | 1 cut(s) | 839 |
| BfrI | CTTAAG | 1 cut(s) | 116 |
| BfuAI | ACCTGC | 1 cut(s) | 169 |
| BisI | GCNGC | 2 cut(s) | 610, 761 |
| BlsI | GCNGC | 2 cut(s) | 611, 762 |
| Bme1390I | CCNGG | 3 cut(s) | 407, 730, 1063 |
| Bme18I | GGWCC | 5 cut(s) | 286, 577, 985, 1276, 1340 |
| BmgT120I | GGNCC | 6 cut(s) | 286, 577, 799, 985, 1276, 1340 |
| BmiI | GGNNCC | 6 cut(s) | 320, 578, 579, 800, 987, 1278 |
| BmrFI | CCNGG | 3 cut(s) | 407, 730, 1063 |
| BmsI | GCATC | 2 cut(s) | 616, 1303 |
| BpiI | GAAGAC | 1 cut(s) | 1270 |
| BpuMI | CCSGG | 2 cut(s) | 407, 1063 |
| BsaAI | YACGTR | 1 cut(s) | 337 |
| BsaJI | CCNNGG | 2 cut(s) | 1062, 1145 |
| BsaWI | WCCGGW | 1 cut(s) | 711 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 447 |
| Bse118I | RCCGGY | 1 cut(s) | 711 |
| Bse1I | ACTGG | 1 cut(s) | 1306 |
| Bse21I | CCTNAGG | 2 cut(s) | 282, 981 |
| Bse3DI | GCAATG | 3 cut(s) | 595, 669, 1207 |
| BseBI | CCWGG | 1 cut(s) | 730 |
| BseDI | CCNNGG | 2 cut(s) | 1062, 1145 |
| BseGI | GGATG | 2 cut(s) | 1141, 1217 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 447 |
| BseMI | GCAATG | 3 cut(s) | 595, 669, 1207 |
| BseMII | CTCAG | 2 cut(s) | 119, 995 |
| BseNI | ACTGG | 1 cut(s) | 1306 |
| BseXI | GCAGC | 1 cut(s) | 621 |
| Bsh1285I | CGRYCG | 1 cut(s) | 716 |
| BshFI | GGCC | 3 cut(s) | 114, 728, 801 |
| BshTI | ACCGGT | 1 cut(s) | 711 |
| BsiEI | CGRYCG | 1 cut(s) | 716 |
| BsiSI | CCGG | 4 cut(s) | 407, 712, 792, 1062 |
| BslFI | GGGAC | 2 cut(s) | 563, 1262 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 447 |
| BsmAI | GTCTC | 1 cut(s) | 60 |
| BsmFI | GGGAC | 2 cut(s) | 563, 1262 |
| BsnI | GGCC | 3 cut(s) | 114, 728, 801 |
| Bsp1407I | TGTACA | 1 cut(s) | 1027 |
| Bsp143I | GATC | 4 cut(s) | 277, 318, 951, 976 |
| BspACI | CCGC | 2 cut(s) | 760, 1205 |
| BspANI | GGCC | 3 cut(s) | 114, 728, 801 |
| BspCNI | CTCAG | 2 cut(s) | 120, 994 |
| BspHI | TCATGA | 1 cut(s) | 948 |
| BspLI | GGNNCC | 6 cut(s) | 320, 578, 579, 800, 987, 1278 |
| BspMI | ACCTGC | 1 cut(s) | 169 |
| BspPI | GGATC | 4 cut(s) | 272, 313, 326, 971 |
| BspTI | CTTAAG | 1 cut(s) | 116 |
| BsrDI | GCAATG | 3 cut(s) | 595, 669, 1207 |
| BsrFI | RCCGGY | 1 cut(s) | 711 |
| BsrGI | TGTACA | 1 cut(s) | 1027 |
| BsrI | ACTGG | 1 cut(s) | 1306 |
| BssAI | RCCGGY | 1 cut(s) | 711 |
| BssECI | CCNNGG | 2 cut(s) | 1062, 1145 |
| BssMI | GATC | 4 cut(s) | 277, 318, 951, 976 |
| BssT1I | CCWWGG | 1 cut(s) | 1145 |
| Bst2UI | CCWGG | 1 cut(s) | 730 |
| Bst4CI | ACNGT | 6 cut(s) | 151, 328, 350, 446, 586, 1285 |
| BstAFI | CTTAAG | 1 cut(s) | 116 |
| BstAUI | TGTACA | 1 cut(s) | 1027 |
| BstBAI | YACGTR | 1 cut(s) | 337 |
| BstDEI | CTNAG | 6 cut(s) | 88, 128, 282, 387, 981, 1022 |
| BstF5I | GGATG | 2 cut(s) | 1141, 1217 |
| BstH2I | RGCGCY | 1 cut(s) | 839 |
| BstHHI | GCGC | 4 cut(s) | 703, 770, 838, 1368 |
| BstKTI | GATC | 4 cut(s) | 280, 321, 954, 979 |
| BstMAI | GTCTC | 1 cut(s) | 60 |
| BstMBI | GATC | 4 cut(s) | 277, 318, 951, 976 |
| BstMCI | CGRYCG | 1 cut(s) | 716 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 170, 606 |
| BstNI | CCWGG | 1 cut(s) | 730 |
| BstSCI | CCNGG | 3 cut(s) | 405, 728, 1061 |
| BstSNI | TACGTA | 1 cut(s) | 337 |
| BstV1I | GCAGC | 1 cut(s) | 621 |
| BstV2I | GAAGAC | 1 cut(s) | 1270 |
| BstX2I | RGATCY | 3 cut(s) | 277, 318, 976 |
| BstYI | RGATCY | 3 cut(s) | 277, 318, 976 |
| Bsu36I | CCTNAGG | 2 cut(s) | 282, 981 |
| BsuRI | GGCC | 3 cut(s) | 114, 728, 801 |
| BtsCI | GGATG | 2 cut(s) | 1141, 1217 |
| BtsI | GCAGTG | 1 cut(s) | 763 |
| BtsIMutI | CAGTG | 3 cut(s) | 355, 763, 1299 |
| BveI | ACCTGC | 1 cut(s) | 169 |
| CciI | TCATGA | 1 cut(s) | 948 |
| CfoI | GCGC | 4 cut(s) | 703, 770, 838, 1368 |
| Cfr10I | RCCGGY | 1 cut(s) | 711 |
| Cfr13I | GGNCC | 6 cut(s) | 286, 577, 799, 985, 1276, 1340 |
| Csp6I | GTAC | 3 cut(s) | 329, 780, 1028 |
| CspAI | ACCGGT | 1 cut(s) | 711 |
| CviAII | CATG | 5 cut(s) | 250, 613, 688, 949, 1312 |
| CviQI | GTAC | 3 cut(s) | 329, 780, 1028 |
| DdeI | CTNAG | 6 cut(s) | 88, 128, 282, 387, 981, 1022 |
| DpnI | GATC | 4 cut(s) | 279, 320, 953, 978 |
| DpnII | GATC | 4 cut(s) | 277, 318, 951, 976 |
| DraI | TTTAAA | 2 cut(s) | 993, 1272 |
| EaeI | YGGCCR | 1 cut(s) | 726 |
| Eco105I | TACGTA | 1 cut(s) | 337 |
| Eco130I | CCWWGG | 1 cut(s) | 1145 |
| Eco147I | AGGCCT | 1 cut(s) | 114 |
| Eco47I | GGWCC | 5 cut(s) | 286, 577, 985, 1276, 1340 |
| Eco57I | CTGAAG | 1 cut(s) | 897 |
| Eco81I | CCTNAGG | 2 cut(s) | 282, 981 |
| EcoO109I | RGGNCCY | 4 cut(s) | 286, 577, 985, 1276 |
| EcoRI | GAATTC | 2 cut(s) | 663, 1017 |
| EcoRII | CCWGG | 1 cut(s) | 728 |
| EcoT14I | CCWWGG | 1 cut(s) | 1145 |
| ErhI | CCWWGG | 1 cut(s) | 1145 |
| FaeI | CATG | 5 cut(s) | 253, 616, 691, 952, 1315 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 553, 585, 1252, 1284 |
| FaqI | GGGAC | 2 cut(s) | 563, 1262 |
| FatI | CATG | 5 cut(s) | 249, 612, 687, 948, 1311 |
| FblI | GTMKAC | 3 cut(s) | 146, 373, 1072 |
| Fnu4HI | GCNGC | 2 cut(s) | 610, 761 |
| FokI | GGATG | 2 cut(s) | 1128, 1204 |
| Fsp4HI | GCNGC | 2 cut(s) | 610, 761 |
| FspBI | CTAG | 2 cut(s) | 771, 888 |
| GlaI | GCGC | 4 cut(s) | 702, 769, 837, 1367 |
| GluI | GCNGC | 2 cut(s) | 610, 761 |
| HaeII | RGCGCY | 1 cut(s) | 839 |
| HaeIII | GGCC | 3 cut(s) | 114, 728, 801 |
| HapII | CCGG | 4 cut(s) | 407, 712, 792, 1062 |
| HhaI | GCGC | 4 cut(s) | 703, 770, 838, 1368 |
| Hin1II | CATG | 5 cut(s) | 253, 616, 691, 952, 1315 |
| Hin6I | GCGC | 4 cut(s) | 701, 768, 836, 1366 |
| HinP1I | GCGC | 4 cut(s) | 701, 768, 836, 1366 |
| HinfI | GANTC | 2 cut(s) | 234, 933 |
| HpaII | CCGG | 4 cut(s) | 407, 712, 792, 1062 |
| HphI | GGTGA | 5 cut(s) | 670, 670, 928, 1219, 1367 |
| Hpy166II | GTNNAC | 7 cut(s) | 147, 374, 511, 1028, 1030, 1073, 1210 |
| Hpy188I | TCNGA | 4 cut(s) | 65, 260, 916, 1055 |
| Hpy188III | TCNNGA | 7 cut(s) | 428, 470, 888, 949, 983, 1319, 1343 |
| Hpy8I | GTNNAC | 7 cut(s) | 147, 374, 511, 1028, 1030, 1073, 1210 |
| Hpy99I | CGWCG | 2 cut(s) | 260, 318 |
| HpyAV | CCTTC | 5 cut(s) | 472, 476, 632, 789, 1175 |
| HpyCH4III | ACNGT | 6 cut(s) | 151, 328, 350, 446, 586, 1285 |
| HpyCH4IV | ACGT | 2 cut(s) | 336, 817 |
| HpyCH4V | TGCA | 6 cut(s) | 173, 215, 401, 455, 824, 872 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 170, 606 |
| HpyF3I | CTNAG | 6 cut(s) | 88, 128, 282, 387, 981, 1022 |
| HpySE526I | ACGT | 2 cut(s) | 336, 817 |
| Hsp92II | CATG | 5 cut(s) | 253, 616, 691, 952, 1315 |
| HspAI | GCGC | 4 cut(s) | 701, 768, 836, 1366 |
| KflI | GGGWCCC | 1 cut(s) | 577 |
| Kzo9I | GATC | 4 cut(s) | 277, 318, 951, 976 |
| LmnI | GCTCC | 2 cut(s) | 260, 642 |
| Lsp1109I | GCAGC | 1 cut(s) | 621 |
| LweI | GCATC | 2 cut(s) | 616, 1303 |
| MaeI | CTAG | 2 cut(s) | 771, 888 |
| MaeII | ACGT | 2 cut(s) | 336, 817 |
| MaeIII | GTNAC | 4 cut(s) | 235, 676, 808, 934 |
| MalI | GATC | 4 cut(s) | 279, 320, 953, 978 |
| MboI | GATC | 4 cut(s) | 277, 318, 951, 976 |
| MboII | GAAGA | 5 cut(s) | 287, 545, 986, 1244, 1275 |
| MflI | RGATCY | 3 cut(s) | 277, 318, 976 |
| MlsI | TGGCCA | 1 cut(s) | 728 |
| MluNI | TGGCCA | 1 cut(s) | 728 |
| MlyI | GAGTC | 2 cut(s) | 243, 942 |
| MmeI | TCCRAC | 3 cut(s) | 238, 583, 1315 |
| MnlI | CCTC | 8 cut(s) | 7, 104, 477, 479, 550, 990, 1178, 1331 |
| Mox20I | TGGCCA | 1 cut(s) | 728 |
| MroXI | GAANNNNTTC | 3 cut(s) | 537, 910, 1236 |
| MscI | TGGCCA | 1 cut(s) | 728 |
| MseI | TTAA | 8 cut(s) | 96, 117, 435, 572, 647, 992, 1134, 1271 |
| Msp20I | TGGCCA | 1 cut(s) | 728 |
| MspCI | CTTAAG | 1 cut(s) | 116 |
| MspI | CCGG | 4 cut(s) | 407, 712, 792, 1062 |
| MspR9I | CCNGG | 3 cut(s) | 407, 730, 1063 |
| MvaI | CCWGG | 1 cut(s) | 730 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 170, 606 |
| NciI | CCSGG | 2 cut(s) | 407, 1063 |
| NdeII | GATC | 4 cut(s) | 277, 318, 951, 976 |
| NlaIII | CATG | 5 cut(s) | 253, 616, 691, 952, 1315 |
| NlaIV | GGNNCC | 6 cut(s) | 320, 578, 579, 800, 987, 1278 |
| NmuCI | GTSAC | 4 cut(s) | 235, 676, 808, 934 |
| PagI | TCATGA | 1 cut(s) | 948 |
| PaqCI | CACCTGC | 1 cut(s) | 169 |
| PceI | AGGCCT | 1 cut(s) | 114 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 342 |
| PdmI | GAANNNNTTC | 3 cut(s) | 537, 910, 1236 |
| PinAI | ACCGGT | 1 cut(s) | 711 |
| PkrI | GCNGC | 2 cut(s) | 611, 762 |
| PleI | GAGTC | 2 cut(s) | 242, 941 |
| PpsI | GAGTC | 2 cut(s) | 242, 941 |
| Ppu21I | YACGTR | 1 cut(s) | 337 |
| PpuMI | RGGWCCY | 4 cut(s) | 286, 577, 985, 1276 |
| Psp5II | RGGWCCY | 4 cut(s) | 286, 577, 985, 1276 |
| Psp6I | CCWGG | 1 cut(s) | 728 |
| PspGI | CCWGG | 1 cut(s) | 728 |
| PspN4I | GGNNCC | 6 cut(s) | 320, 578, 579, 800, 987, 1278 |
| PspPI | GGNCC | 6 cut(s) | 286, 577, 799, 985, 1276, 1340 |
| PspPPI | RGGWCCY | 4 cut(s) | 286, 577, 985, 1276 |
| PsuI | RGATCY | 3 cut(s) | 277, 318, 976 |
| RsaI | GTAC | 3 cut(s) | 330, 781, 1029 |
| RsaNI | GTAC | 3 cut(s) | 329, 780, 1028 |
| SaqAI | TTAA | 8 cut(s) | 96, 117, 435, 572, 647, 992, 1134, 1271 |
| SatI | GCNGC | 2 cut(s) | 610, 761 |
| Sau3AI | GATC | 4 cut(s) | 277, 318, 951, 976 |
| Sau96I | GGNCC | 6 cut(s) | 286, 577, 799, 985, 1276, 1340 |
| SchI | GAGTC | 2 cut(s) | 243, 942 |
| ScrFI | CCNGG | 3 cut(s) | 407, 730, 1063 |
| SfaNI | GCATC | 2 cut(s) | 616, 1303 |
| SinI | GGWCC | 5 cut(s) | 286, 577, 985, 1276, 1340 |
| SmlI | CTYRAG | 1 cut(s) | 116 |
| SmoI | CTYRAG | 1 cut(s) | 116 |
| SnaBI | TACGTA | 1 cut(s) | 337 |
| SseBI | AGGCCT | 1 cut(s) | 114 |
| SsiI | CCGC | 2 cut(s) | 760, 1205 |
| SspI | AATATT | 1 cut(s) | 1092 |
| SspMI | CTAG | 2 cut(s) | 771, 888 |
| StuI | AGGCCT | 1 cut(s) | 114 |
| StyD4I | CCNGG | 3 cut(s) | 405, 728, 1061 |
| StyI | CCWWGG | 1 cut(s) | 1145 |
| TaaI | ACNGT | 6 cut(s) | 151, 328, 350, 446, 586, 1285 |
| TaiI | ACGT | 2 cut(s) | 339, 820 |
| TaqI | TCGA | 1 cut(s) | 313 |
| TatI | WGTACW | 1 cut(s) | 1027 |
| TauI | GCSGC | 1 cut(s) | 763 |
| Tru1I | TTAA | 8 cut(s) | 96, 117, 435, 572, 647, 992, 1134, 1271 |
| Tru9I | TTAA | 8 cut(s) | 96, 117, 435, 572, 647, 992, 1134, 1271 |
| TscAI | CASTG | 3 cut(s) | 355, 770, 1306 |
| TseFI | GTSAC | 4 cut(s) | 235, 676, 808, 934 |
| TseI | GCWGC | 1 cut(s) | 609 |
| Tsp45I | GTSAC | 4 cut(s) | 235, 676, 808, 934 |
| TspDTI | ATGAA | 4 cut(s) | 91, 308, 491, 1338 |
| TspGWI | ACGGA | 1 cut(s) | 331 |
| TspRI | CASTG | 3 cut(s) | 355, 770, 1306 |
| Vha464I | CTTAAG | 1 cut(s) | 116 |
| VpaK11BI | GGWCC | 5 cut(s) | 286, 577, 985, 1276, 1340 |
| XapI | RAATTY | 5 cut(s) | 40, 357, 423, 663, 1017 |
| XbaI | TCTAGA | 1 cut(s) | 887 |
| XmiI | GTMKAC | 3 cut(s) | 146, 373, 1072 |
| XmnI | GAANNNNTTC | 3 cut(s) | 537, 910, 1236 |
| XspI | CTAG | 2 cut(s) | 771, 888 |