RLG00000010059

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001308
Physical Location & Seq
Reverse (-)
62509733 .. 62510199
467 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010059

Sequence Viewer

Length: 366 bp
ATGACTCGGCAATCTCTTCCACCCACGGCGTCACTAGTCTCCCCAATCCTCCGGTATCAGATTAAACGTCGCCGCCCTCGGCTTTCCCTTCAGCTTCAGCCACTTGAGCTGCCGAAATCGCCTCCTAAGTCACTCAGGGCTCGTCGTCTAGTAAAGCGCGATGATTGCTCTGCACCGGTGTTTTGGGATGATTCGTCATCGTCGCGGTCTGATCTGGCATTCAATTTCATCTACGAGGTGATGGTTTGGATTGGGCTTTCGAATGGCGTGCACTTTGCTTTCAAGAGGGTGATTCGAATCGTAGCAGAGGTGTTAGATGATGCAGAGAGAGAAAAGGTGCCTATGAGATTGGCTTTGGTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.95

Weight (kDa)

10.4

Isoelectric Point (pI)

70.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 337
AccII CGCG 2 cut(s) 159, 205
AciI CCGC 2 cut(s) 73, 205
AcuI CTGAAG 2 cut(s) 74, 80
AcyI GRCGYC 1 cut(s) 29
AgeI ACCGGT 1 cut(s) 175
AgsI TTSAA 2 cut(s) 223, 283
AhlI ACTAGT 1 cut(s) 34
AluBI AGCT 2 cut(s) 94, 109
AluI AGCT 2 cut(s) 94, 109
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 43
Alw44I GTGCAC 1 cut(s) 269
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 109
AsiGI ACCGGT 1 cut(s) 175
AspLEI GCGC 1 cut(s) 159
AsuHPI GGTGA 2 cut(s) 250, 301
AsuII TTCGAA 2 cut(s) 260, 295
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 96
BccI CCATC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 42
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BcoDI GTCTC 1 cut(s) 43
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 149
BisI GCNGC 2 cut(s) 73, 110
BlsI GCNGC 2 cut(s) 74, 111
BmiI GGNNCC 1 cut(s) 339
BmsI GCATC 1 cut(s) 310
Bpu14I TTCGAA 2 cut(s) 260, 295
BpuEI CTTGAG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 24, 77
BsaWI WCCGGW 2 cut(s) 51, 175
Bse118I RCCGGY 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 24, 77
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 296
BseMII CTCAG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 2 cut(s) 159, 205
BshNI GGYRCC 1 cut(s) 337
BshTI ACCGGT 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 52, 176
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 218
Bsp119I TTCGAA 2 cut(s) 260, 295
Bsp1286I GDGCHC 2 cut(s) 142, 273
Bsp143I GATC 1 cut(s) 211
BspACI CCGC 2 cut(s) 73, 205
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 2 cut(s) 159, 205
BspLI GGNNCC 1 cut(s) 339
BspT104I TTCGAA 2 cut(s) 260, 295
BspT107I GGYRCC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 24, 77
BssMI GATC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 29
BstBI TTCGAA 2 cut(s) 260, 295
BstC8I GCNNGC 1 cut(s) 269
BstDEI CTNAG 2 cut(s) 126, 134
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 2 cut(s) 159, 205
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 3 cut(s) 106, 118, 165
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 2 cut(s) 159, 205
BstV1I GCAGC 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 269
CfoI GCGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 175
CseI GACGC 1 cut(s) 18
CspAI ACCGGT 1 cut(s) 175
CviJI RGCY 7 cut(s) 82, 94, 100, 109, 140, 256, 353
CviKI_1 RGCY 7 cut(s) 82, 94, 100, 109, 140, 256, 353
DdeI CTNAG 2 cut(s) 126, 134
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 142
Eco57I CTGAAG 2 cut(s) 74, 80
EcoT38I GRGCYC 1 cut(s) 142
FaiI YATR 1 cut(s) 344
Fnu4HI GCNGC 2 cut(s) 73, 110
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 2 cut(s) 73, 110
FspBI CTAG 2 cut(s) 35, 149
GlaI GCGC 1 cut(s) 158
GluI GCNGC 2 cut(s) 73, 110
HapII CCGG 2 cut(s) 52, 176
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 29
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HinfI GANTC 4 cut(s) 4, 191, 292, 297
HpaII CCGG 2 cut(s) 52, 176
HphI GGTGA 2 cut(s) 250, 301
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 60, 211
Hpy188III TCNNGA 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 3 cut(s) 72, 147, 205
HpyAV CCTTC 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 173, 271, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 118, 165
HpyF3I CTNAG 2 cut(s) 126, 134
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 29
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 211
LpnPI CCDG 4 cut(s) 65, 121, 189, 200
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 1 cut(s) 310
MaeI CTAG 2 cut(s) 35, 149
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 2 cut(s) 30, 129
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 142, 273
MluCI AATT 1 cut(s) 223
MnlI CCTC 6 cut(s) 59, 87, 132, 229, 279, 301
MseI TTAA 1 cut(s) 63
MspI CCGG 2 cut(s) 52, 176
Mva1269I GAATGC 1 cut(s) 218
MvnI CGCG 2 cut(s) 159, 205
MwoI GCNNNNNNNGC 3 cut(s) 106, 118, 165
NdeII GATC 1 cut(s) 211
NlaIV GGNNCC 1 cut(s) 339
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 30, 129
NspV TTCGAA 2 cut(s) 260, 295
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 3 cut(s) 191, 292, 297
PinAI ACCGGT 1 cut(s) 175
PkrI GCNGC 2 cut(s) 74, 111
PspN4I GGNNCC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 2 cut(s) 73, 110
Sau3AI GATC 1 cut(s) 211
SduI GDGCHC 2 cut(s) 142, 273
SetI ASST 6 cut(s) 70, 96, 111, 240, 312, 339
SfaNI GCATC 1 cut(s) 310
SfuI TTCGAA 2 cut(s) 260, 295
SgrAI CRCCGGYG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 1 cut(s) 223
SsiI CCGC 2 cut(s) 73, 205
SspMI CTAG 2 cut(s) 35, 149
TaiI ACGT 1 cut(s) 70
TaqI TCGA 2 cut(s) 260, 295
TasI AATT 1 cut(s) 223
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 3 cut(s) 191, 292, 297
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TseFI GTSAC 2 cut(s) 30, 129
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 2 cut(s) 30, 129
TspDTI ATGAA 1 cut(s) 217
VneI GTGCAC 1 cut(s) 269
XspI CTAG 2 cut(s) 35, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.