Rroxscaffold_4G00328740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001308
Physical Location & Seq
Reverse (-)
61821529 .. 61825347
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328740.1

Sequence Viewer

Length: 570 bp
ATGTCCGACCAAACTTGGATCCGCCCCAAGATCCAAGCCTTTACACCACCTCCATTGGGCTACCATAATGGGACCCCAAAGTCACACCACCATCCCGTGACCCACCACAAGAGTGGTCACAATCCGACAACCGACAACAATCCACTCCGTCCACCTCCTCTTTCACCGAATAGACCACGTCATCGATCCGAGCAACGCTCCGATCAATCTAAAGCCTCCATCCTTGCCGAGCGCGTGAAGCGAGGCAAAGGTAGTTCATTTGCTCGGCGTGCTTCCGCATTGCTCGCACCGGTGTTTTGGGATGATTCGTCGCCGTCGCTTGATCCGGCATTCAATTTCATCTACAAGGTGATGGTTTGGATTAGGCTTTCGAATGGAGTGCACTTTGCTTTCAAGAGGGTGATTCGAATCGTAGCAGATGTGTTAGATGATGCAAAGAGAGAAAAGGTGCCTATGAGATTGGCTTTGAAACAATACAAAGAAAAGCAACAAGCTATAAAACTAGAAACAGCTCCAGAACAAAGAACAAAGAACAAAGGAACTGATCCCCAACTAGAAATTAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.57

Weight (kDa)

10.33

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
AccB1I GGYRCC 1 cut(s) 448
AccII CGCG 1 cut(s) 234
AciI CCGC 2 cut(s) 22, 276
AclWI GGATC 6 cut(s) 13, 25, 26, 180, 317, 539
AfiI CCNNNNNNNGG 1 cut(s) 56
AgeI ACCGGT 1 cut(s) 289
AgsI TTSAA 3 cut(s) 334, 394, 469
AjiI CACGTC 1 cut(s) 179
AleI CACNNNNGTG 1 cut(s) 111
AluBI AGCT 2 cut(s) 494, 512
AluI AGCT 2 cut(s) 494, 512
Alw21I GWGCWC 1 cut(s) 384
Alw44I GTGCAC 1 cut(s) 380
AlwI GGATC 6 cut(s) 13, 25, 26, 180, 317, 539
ApaLI GTGCAC 1 cut(s) 380
AsiGI ACCGGT 1 cut(s) 289
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 156, 361, 412
AsuII TTCGAA 2 cut(s) 371, 406
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 384
BamHI GGATCC 1 cut(s) 18
BanI GGYRCC 1 cut(s) 448
Bbv12I GWGCWC 1 cut(s) 384
BccI CCATC 3 cut(s) 99, 227, 346
BceAI ACGGC 1 cut(s) 298
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BfaI CTAG 2 cut(s) 503, 554
Bme18I GGWCC 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 20, 73, 74, 450
BmsI GCATC 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 371, 406
Bsa29I ATCGAT 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 407
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse118I RCCGGY 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 278
Bse8I GATNNNNATC 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 184
BseGI GGATG 3 cut(s) 91, 219, 307
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 147
BseSI GKGCMC 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 234
BshNI GGYRCC 1 cut(s) 448
BshTI ACCGGT 1 cut(s) 289
BshVI ATCGAT 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 2 cut(s) 290, 326
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 329
Bsp119I TTCGAA 2 cut(s) 371, 406
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 6 cut(s) 18, 30, 185, 202, 322, 544
BspACI CCGC 2 cut(s) 22, 276
BspDI ATCGAT 1 cut(s) 184
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 4 cut(s) 20, 73, 74, 450
BspPI GGATC 6 cut(s) 13, 25, 26, 180, 317, 539
BspT104I TTCGAA 2 cut(s) 371, 406
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 289
BssAI RCCGGY 1 cut(s) 289
BssMI GATC 6 cut(s) 18, 30, 185, 202, 322, 544
BstBI TTCGAA 2 cut(s) 371, 406
BstC8I GCNNGC 2 cut(s) 270, 285
BstF5I GGATG 3 cut(s) 91, 219, 307
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 6 cut(s) 21, 33, 188, 205, 325, 547
BstMBI GATC 6 cut(s) 18, 30, 185, 202, 322, 544
BstMWI GCNNNNNNNGC 3 cut(s) 238, 269, 284
BstSLI GKGCMC 1 cut(s) 384
BstUI CGCG 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 18, 30
BstXI CCANNNNNNTGG 1 cut(s) 113
BstYI RGATCY 2 cut(s) 18, 30
Bsu15I ATCGAT 1 cut(s) 184
BsuTUI ATCGAT 1 cut(s) 184
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 3 cut(s) 91, 219, 307
Cac8I GCNNGC 2 cut(s) 270, 285
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 184
CspAI ACCGGT 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviJI RGCY 7 cut(s) 38, 60, 215, 367, 464, 494, 512
CviKI_1 RGCY 7 cut(s) 38, 60, 215, 367, 464, 494, 512
DpnI GATC 6 cut(s) 20, 32, 187, 204, 324, 546
DpnII GATC 6 cut(s) 18, 30, 185, 202, 322, 544
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
EciI GGCGGA 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 72
FaiI YATR 3 cut(s) 66, 455, 497
FaqI GGGAC 1 cut(s) 85
FokI GGATG 3 cut(s) 78, 206, 314
FspBI CTAG 2 cut(s) 503, 554
GlaI GCGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 498
HapII CCGG 2 cut(s) 290, 326
HhaI GCGC 1 cut(s) 234
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 3 cut(s) 305, 403, 408
HpaII CCGG 2 cut(s) 290, 326
HphI GGTGA 3 cut(s) 156, 361, 412
Hpy166II GTNNAC 2 cut(s) 152, 382
Hpy188I TCNGA 4 cut(s) 7, 126, 190, 202
Hpy188III TCNNGA 2 cut(s) 394, 515
Hpy8I GTNNAC 2 cut(s) 152, 382
Hpy99I CGWCG 2 cut(s) 313, 319
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 2 cut(s) 382, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 269, 284
HpySE526I ACGT 1 cut(s) 178
HspAI GCGC 1 cut(s) 232
KflI GGGWCCC 1 cut(s) 72
Kzo9I GATC 6 cut(s) 18, 30, 185, 202, 322, 544
LmnI GCTCC 2 cut(s) 203, 517
LpnPI CCDG 3 cut(s) 303, 339, 528
LweI GCATC 1 cut(s) 421
MaeI CTAG 2 cut(s) 503, 554
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 3 cut(s) 81, 97, 116
MalI GATC 6 cut(s) 20, 32, 187, 204, 324, 546
MboI GATC 6 cut(s) 18, 30, 185, 202, 322, 544
MflI RGATCY 2 cut(s) 18, 30
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 2 cut(s) 334, 558
MmeI TCCRAC 2 cut(s) 30, 149
MnlI CCTC 6 cut(s) 60, 165, 168, 226, 236, 390
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 111
MspI CCGG 2 cut(s) 290, 326
Mva1269I GAATGC 1 cut(s) 329
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 238, 269, 284
NdeII GATC 6 cut(s) 18, 30, 185, 202, 322, 544
NlaIV GGNNCC 4 cut(s) 20, 73, 74, 450
NmeAIII GCCGAG 2 cut(s) 244, 253
NmuCI GTSAC 3 cut(s) 81, 97, 116
NspV TTCGAA 2 cut(s) 371, 406
OliI CACNNNNGTG 1 cut(s) 111
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 3 cut(s) 305, 403, 408
PflFI GACNNNGTC 1 cut(s) 177
PinAI ACCGGT 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 20, 73, 74, 450
PspPI GGNCC 1 cut(s) 72
PspPPI RGGWCCY 1 cut(s) 72
PsuI RGATCY 2 cut(s) 18, 30
PsyI GACNNNGTC 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 1 cut(s) 561
Sau3AI GATC 6 cut(s) 18, 30, 185, 202, 322, 544
Sau96I GGNCC 1 cut(s) 72
SduI GDGCHC 1 cut(s) 384
SetI ASST 8 cut(s) 52, 157, 181, 253, 351, 450, 496, 514
SfaNI GCATC 1 cut(s) 421
SfuI TTCGAA 2 cut(s) 371, 406
SgrAI CRCCGGYG 1 cut(s) 289
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 111
Sse9I AATT 2 cut(s) 334, 558
SsiI CCGC 2 cut(s) 22, 276
SspMI CTAG 2 cut(s) 503, 554
TaiI ACGT 1 cut(s) 181
TaqI TCGA 3 cut(s) 184, 371, 406
TasI AATT 2 cut(s) 334, 558
TfiI GAWTC 3 cut(s) 305, 403, 408
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TseFI GTSAC 3 cut(s) 81, 97, 116
Tsp45I GTSAC 3 cut(s) 81, 97, 116
TspDTI ATGAA 2 cut(s) 246, 328
TspGWI ACGGA 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 177
VneI GTGCAC 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XspI CTAG 2 cut(s) 503, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.