RLG00000013492

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001308
Physical Location & Seq
Reverse (-)
32348707 .. 32349333
627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013492

Sequence Viewer

Length: 507 bp
ATGAGGAACCTCAAGCTTCCGATCATCGAGCCTGGCTGCCAGGAGCTCAAAATCAAAGCCGAGTCGCTCTCCTCGAGCAGTACTCCAATATTTCGTAGTCTCAATGTCTGGAAGGAGTCTGAATCGGAGAATGGCGGCGGTAGTGGCGAGCAGTTGAGACCTGAAGCATCCGATGCTTTTGAATCAGCTCCGCCACACGACTCCCAGCCTCGGCATCGTTGTCGTGGCCTTTGTGGTTGCTTGGTCGGCGAGCAGTTGTACAACCTCGGTGGATTAATGAGCGGCCAGTGCGAGGCAGAGGTAGTTCATTTGCTCGGCTTGCTTCCAGATTGCTTCGCACCGATGTTTTGGGATGATCCGTCGTCGTCGCGATCCAATCTGGCATTCAATTTCGTCTACGAGGTGATGGTTTGGATTGGACTTTCGAATTGCGTGCACTTTGCTTTCAAGAGGATGATTCGAATCATGGCAGATGGGTTAGATGACGCAGAGAGAATGATGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.66

Weight (kDa)

5.07

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 282
AccI GTMKAC 1 cut(s) 396
AccII CGCG 1 cut(s) 370
AciI CCGC 4 cut(s) 135, 138, 191, 282
AclWI GGATC 2 cut(s) 350, 366
AcoI YGGCCR 1 cut(s) 283
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 2 cut(s) 82, 260
AfiI CCNNNNNNNGG 2 cut(s) 210, 292
AgsI TTSAA 3 cut(s) 182, 388, 448
AjnI CCWGG 2 cut(s) 31, 39
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 3 cut(s) 16, 46, 188
AluI AGCT 3 cut(s) 16, 46, 188
Alw21I GWGCWC 2 cut(s) 48, 438
Alw26I GTCTC 2 cut(s) 104, 151
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 2 cut(s) 350, 366
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 2 cut(s) 226, 283
ApaLI GTGCAC 1 cut(s) 434
ApeKI GCWGC 1 cut(s) 36
AseI ATTAAT 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 415
AsuII TTCGAA 2 cut(s) 425, 460
AvaI CYCGRG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 48, 438
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 400, 467
BcgI CGANNNNNNTGC 4 cut(s) 203, 237, 415, 449
BciT130I CCWGG 2 cut(s) 33, 41
BcoDI GTCTC 2 cut(s) 104, 151
BfaI CTAG 1 cut(s) 505
BisI GCNGC 3 cut(s) 37, 136, 283
BlsI GCNGC 3 cut(s) 38, 137, 284
BmcAI AGTACT 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 33, 41
BmeT110I CYCGRG 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 2 cut(s) 33, 41
BmsI GCATC 4 cut(s) 163, 176, 223, 489
BplI GAGNNNNNCTC 4 cut(s) 53, 85, 67, 99
Bpu14I TTCGAA 2 cut(s) 425, 460
BsaBI GATNNNNATC 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 2 cut(s) 209, 265
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 292
Bse1I ACTGG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 2 cut(s) 33, 41
BseDI CCNNGG 2 cut(s) 209, 265
BseGI GGATG 3 cut(s) 167, 358, 459
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 210, 292
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 61
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 228, 285
BsiHKAI GWGCWC 2 cut(s) 48, 438
BsiHKCI CYCGRG 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 210, 292
BsmAI GTCTC 2 cut(s) 104, 151
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 228, 285
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 2 cut(s) 425, 460
Bsp1286I GDGCHC 2 cut(s) 48, 438
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 3 cut(s) 21, 355, 371
Bsp68I TCGCGA 1 cut(s) 370
BspACI CCGC 4 cut(s) 135, 138, 191, 282
BspANI GGCC 2 cut(s) 228, 285
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 8
BspPI GGATC 2 cut(s) 350, 366
BspT104I TTCGAA 2 cut(s) 425, 460
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 1 cut(s) 282
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 209, 265
BssMI GATC 3 cut(s) 21, 355, 371
Bst2UI CCWGG 2 cut(s) 33, 41
BstAPI GCANNNNNTGC 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 258
BstBI TTCGAA 2 cut(s) 425, 460
BstC8I GCNNGC 4 cut(s) 149, 251, 320, 434
BstF5I GGATG 3 cut(s) 167, 358, 459
BstFNI CGCG 1 cut(s) 370
BstKTI GATC 3 cut(s) 24, 358, 374
BstMAI GTCTC 2 cut(s) 104, 151
BstMBI GATC 3 cut(s) 21, 355, 371
BstMWI GCNNNNNNNGC 5 cut(s) 144, 173, 246, 288, 319
BstNI CCWGG 2 cut(s) 33, 41
BstSCI CCNGG 2 cut(s) 31, 39
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 228, 285
BtsCI GGATG 3 cut(s) 167, 358, 459
BtsIMutI CAGTG 1 cut(s) 293
BtuMI TCGCGA 1 cut(s) 370
Cac8I GCNNGC 4 cut(s) 149, 251, 320, 434
CseI GACGC 1 cut(s) 494
Csp6I GTAC 2 cut(s) 81, 259
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 1 cut(s) 466
CviQI GTAC 2 cut(s) 81, 259
DpnI GATC 3 cut(s) 23, 357, 373
DpnII GATC 3 cut(s) 21, 355, 371
EaeI YGGCCR 1 cut(s) 283
EciI GGCGGA 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 31, 39
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 1 cut(s) 469
FaiI YATR 1 cut(s) 467
FatI CATG 1 cut(s) 465
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 3 cut(s) 37, 136, 283
FokI GGATG 3 cut(s) 154, 365, 466
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 37, 136, 283
FspBI CTAG 1 cut(s) 505
GluI GCNGC 3 cut(s) 37, 136, 283
GsaI CCCAGC 1 cut(s) 208
HaeIII GGCC 2 cut(s) 228, 285
HgaI GACGC 1 cut(s) 494
Hin1II CATG 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 7 cut(s) 62, 116, 122, 182, 200, 457, 462
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 2 cut(s) 397, 436
Hpy188I TCNGA 4 cut(s) 21, 121, 127, 172
Hpy188III TCNNGA 4 cut(s) 109, 326, 369, 448
Hpy8I GTNNAC 2 cut(s) 397, 436
Hpy99I CGWCG 3 cut(s) 364, 367, 370
HpyAV CCTTC 1 cut(s) 106
HpyCH4V TGCA 1 cut(s) 436
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 173, 246, 288, 319
Hsp92II CATG 1 cut(s) 469
Kzo9I GATC 3 cut(s) 21, 355, 371
LmnI GCTCC 2 cut(s) 43, 193
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 4 cut(s) 163, 176, 223, 489
MaeI CTAG 1 cut(s) 505
MalI GATC 3 cut(s) 23, 357, 373
MbiI CCGCTC 1 cut(s) 282
MboI GATC 3 cut(s) 21, 355, 371
MhlI GDGCHC 2 cut(s) 48, 438
MluCI AATT 2 cut(s) 388, 427
MlyI GAGTC 3 cut(s) 71, 125, 194
MnlI CCTC 8 cut(s) 20, 82, 219, 275, 286, 292, 394, 444
MseI TTAA 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 33, 41
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 2 cut(s) 33, 41
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 5 cut(s) 144, 173, 246, 288, 319
NdeII GATC 3 cut(s) 21, 355, 371
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 1 cut(s) 8
NmeAIII GCCGAG 3 cut(s) 85, 190, 294
NruI TCGCGA 1 cut(s) 370
NspV TTCGAA 2 cut(s) 425, 460
PaeR7I CTCGAG 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 4 cut(s) 122, 182, 457, 462
PkrI GCNGC 3 cut(s) 38, 137, 284
PleI GAGTC 3 cut(s) 70, 124, 194
PpsI GAGTC 3 cut(s) 70, 124, 194
PshBI ATTAAT 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 31, 39
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 2 cut(s) 31, 39
PspN4I GGNNCC 1 cut(s) 8
PspXI VCTCGAGB 1 cut(s) 73
RruI TCGCGA 1 cut(s) 370
RsaI GTAC 2 cut(s) 82, 260
RsaNI GTAC 2 cut(s) 81, 259
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 3 cut(s) 37, 136, 283
Sau3AI GATC 3 cut(s) 21, 355, 371
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 3 cut(s) 71, 125, 194
ScrFI CCNGG 2 cut(s) 33, 41
SduI GDGCHC 2 cut(s) 48, 438
SetI ASST 8 cut(s) 12, 18, 48, 163, 190, 267, 303, 405
SfaNI GCATC 4 cut(s) 163, 176, 223, 489
Sfr274I CTCGAG 1 cut(s) 73
SfuI TTCGAA 2 cut(s) 425, 460
SlaI CTCGAG 1 cut(s) 73
SmlI CTYRAG 2 cut(s) 11, 73
SmoI CTYRAG 2 cut(s) 11, 73
Sse9I AATT 2 cut(s) 388, 427
SsiI CCGC 4 cut(s) 135, 138, 191, 282
SspI AATATT 1 cut(s) 90
SspMI CTAG 1 cut(s) 505
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 31, 39
TaqI TCGA 4 cut(s) 27, 74, 425, 460
TasI AATT 2 cut(s) 388, 427
TatI WGTACW 2 cut(s) 80, 258
TauI GCSGC 2 cut(s) 138, 285
TfiI GAWTC 4 cut(s) 122, 182, 457, 462
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 1 cut(s) 293
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 434
VspI ATTAAT 1 cut(s) 275
XhoI CTCGAG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 396
XspI CTAG 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.