Rh4DG244700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001308
Physical Location & Seq
Forward (+)
45679728 .. 45680670
943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG244700.1

Sequence Viewer

Length: 381 bp
ATGTGCGGAAGCATCTTGATTAGCCCGGCCGATCAAAACGACAGCGTCACTGACCTTCAACCCGACGACTCTGAGCAGCACGAAGGTCTCGAGAATGCAAGGGCTGCTGGTGACGAGGTCCTTGATGATGAGGTAGACCTGGCTGGGCTTGAAATCCTTGACAGAAAAAGGAGAGAACTTGACGAGTTAAAGCTTATCGGTGGAGTGATTGACCCAGGAGACGACATCGGAGACGAGTTGCTCGGTCTTGAGGCTCGACCAATTGAAGCGTCGACCACCGCAATAGAACTAATCGGACCCATTCTTGGAACACAGTCGCAGTCGGCCATGAGGATGTCATGGGAGACGCTGATGCAGGTGGCGACGGGGAGGGTGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.54

Weight (kDa)

4.05

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 2 cut(s) 135, 272
AciI CCGC 2 cut(s) 6, 279
AcoI YGGCCR 2 cut(s) 27, 324
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 3 cut(s) 59, 152, 266
AjnI CCWGG 2 cut(s) 138, 214
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw26I GTCTC 4 cut(s) 92, 213, 225, 338
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 2 cut(s) 27, 324
ApeKI GCWGC 2 cut(s) 76, 104
AspS9I GGNCC 2 cut(s) 118, 296
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 118, 296
BbvI GCAGC 2 cut(s) 88, 91
BciT130I CCWGG 2 cut(s) 140, 216
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 4 cut(s) 92, 213, 225, 338
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 2 cut(s) 77, 105
BlsI GCNGC 2 cut(s) 78, 106
Bme1390I CCNGG 3 cut(s) 26, 140, 216
Bme18I GGWCC 2 cut(s) 118, 296
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 118, 296
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 3 cut(s) 26, 140, 216
BmsI GCATC 2 cut(s) 21, 342
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 305
BseBI CCWGG 2 cut(s) 140, 216
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 63
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 88, 91
BseYI CCCAGC 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 2 cut(s) 29, 326
BsiEI CGRYCG 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 4 cut(s) 92, 213, 225, 338
BsmBI CGTCTC 3 cut(s) 213, 225, 338
BsmI GAATGC 1 cut(s) 100
BsnI GGCC 2 cut(s) 29, 326
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 89
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 2 cut(s) 6, 279
BspANI GGCC 2 cut(s) 29, 326
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 140, 216
Bst4CI ACNGT 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 339
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 4 cut(s) 92, 213, 225, 338
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 2 cut(s) 140, 216
BstSCI CCNGG 3 cut(s) 24, 138, 214
BstV1I GCAGC 2 cut(s) 88, 91
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 2 cut(s) 29, 326
BtsCI GGATG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 48
BveI ACCTGC 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 118, 296
CseI GACGC 3 cut(s) 34, 258, 355
CviAII CATG 2 cut(s) 328, 339
CviJI RGCY 8 cut(s) 24, 29, 104, 143, 148, 193, 254, 326
CviKI_1 RGCY 8 cut(s) 24, 29, 104, 143, 148, 193, 254, 326
DdeI CTNAG 1 cut(s) 72
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
EaeI YGGCCR 2 cut(s) 27, 324
EagI CGGCCG 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 2 cut(s) 118, 296
Eco52I CGGCCG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 138, 214
Esp3I CGTCTC 3 cut(s) 213, 225, 338
FaeI CATG 2 cut(s) 331, 342
FaiI YATR 2 cut(s) 329, 340
FatI CATG 2 cut(s) 327, 338
FblI GTMKAC 2 cut(s) 135, 272
Fnu4HI GCNGC 2 cut(s) 77, 105
FokI GGATG 1 cut(s) 346
Fsp4HI GCNGC 2 cut(s) 77, 105
GluI GCNGC 2 cut(s) 77, 105
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 29, 326
HapII CCGG 1 cut(s) 26
HgaI GACGC 3 cut(s) 34, 258, 355
Hin1II CATG 2 cut(s) 331, 342
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 1 cut(s) 68
HpaII CCGG 1 cut(s) 26
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 136, 273
Hpy188I TCNGA 3 cut(s) 73, 230, 296
Hpy188III TCNNGA 4 cut(s) 16, 89, 91, 248
Hpy8I GTNNAC 2 cut(s) 136, 273
Hpy99I CGWCG 3 cut(s) 68, 274, 367
HpyAV CCTTC 2 cut(s) 65, 77
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 98, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 1 cut(s) 72
Hsp92II CATG 2 cut(s) 331, 342
Kzo9I GATC 1 cut(s) 31
LpnPI CCDG 8 cut(s) 39, 93, 125, 129, 152, 201, 228, 341
Lsp1109I GCAGC 2 cut(s) 88, 91
LweI GCATC 2 cut(s) 21, 342
MaeIII GTNAC 2 cut(s) 46, 110
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MfeI CAATTG 1 cut(s) 261
MluCI AATT 1 cut(s) 261
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 5 cut(s) 109, 124, 244, 324, 363
MseI TTAA 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 3 cut(s) 26, 140, 216
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 100
MvaI CCWGG 2 cut(s) 140, 216
MwoI GCNNNNNNNGC 1 cut(s) 104
NciI CCSGG 1 cut(s) 26
NdeII GATC 1 cut(s) 31
NlaIII CATG 2 cut(s) 331, 342
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 46, 110
PaeR7I CTCGAG 1 cut(s) 89
PaqCI CACCTGC 1 cut(s) 346
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 1 cut(s) 100
PflFI GACNNNGTC 2 cut(s) 44, 116
PkrI GCNGC 2 cut(s) 78, 106
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 118
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 138, 214
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 138, 214
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 2 cut(s) 118, 296
PspPPI RGGWCCY 1 cut(s) 118
PsyI GACNNNGTC 2 cut(s) 44, 116
RseI CAYNNNNRTG 1 cut(s) 332
SalI GTCGAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 2 cut(s) 77, 105
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 2 cut(s) 118, 296
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 3 cut(s) 26, 140, 216
SetI ASST 7 cut(s) 57, 88, 120, 135, 141, 195, 360
SfaNI GCATC 2 cut(s) 21, 342
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 118, 296
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 89, 248
SmoI CTYRAG 2 cut(s) 89, 248
Sse9I AATT 1 cut(s) 261
SsiI CCGC 2 cut(s) 6, 279
StyD4I CCNGG 3 cut(s) 24, 138, 214
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 3 cut(s) 90, 256, 272
TaqII GACCGA 1 cut(s) 233
TasI AATT 1 cut(s) 261
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 2 cut(s) 46, 110
TseI GCWGC 2 cut(s) 76, 104
Tsp45I GTSAC 2 cut(s) 46, 110
TspRI CASTG 1 cut(s) 55
Tth111I GACNNNGTC 2 cut(s) 44, 116
VpaK11BI GGWCC 2 cut(s) 118, 296
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 2 cut(s) 135, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.