Rmu_sc0004342.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004342.1
OG0001308
Physical Location & Seq
Forward (+)
1 .. 3659
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004342.1_g000001.1.cds

Sequence Viewer

Length: 594 bp
gacggcggcggcgaggaggacggcgggttcttcggcggcgacggcccgtttttcaacggcggatggggcggcggcggcggcggtggcgggggttggaatttcgatagattcggagggcataattgggatgattcgtcgtcgtcgcggtccgatccggcgttcgatttcgtctacgaggtgatgggttggattgcgctttcgaattgcgtgcactttgcttttaagagggtgattcgaatcgtggcggatgggttaggcgatgcggagagagagaaggtgcctatgagattggcttcggcatggtaccagatggtactcccactatggatcggtggattcttaagccagaagaggatgggtgaaggtctcgagaatggaagcgctgctggtgacgaggtccttgatgatgaggtagacctggccgggcttgaaatccttgaccagaaaaagagagaacttgacgagttaaagcttaccggtggagtgattgacccaggagaggacatcgaagacgagttgctcggtcttgaggcgcgaatcacggctaccccactcgttctcgacgaccaattgaagagtcgaccaccgcgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.22

Weight (kDa)

4.3

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 2 cut(s) 277, 303
AccI GTMKAC 3 cut(s) 171, 414, 580
AccII CGCG 3 cut(s) 145, 535, 589
AclWI GGATC 2 cut(s) 146, 335
AcoI YGGCCR 1 cut(s) 420
AcsI RAATTY 1 cut(s) 97
AfaI GTAC 2 cut(s) 305, 315
AfeI AGCGCT 1 cut(s) 382
AfiI CCNNNNNNNGG 1 cut(s) 499
AflII CTTAAG 1 cut(s) 340
AgeI ACCGGT 1 cut(s) 476
AgsI TTSAA 3 cut(s) 55, 431, 574
AjnI CCWGG 2 cut(s) 417, 493
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 1 cut(s) 472
AluI AGCT 1 cut(s) 472
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 209
AlwI GGATC 2 cut(s) 146, 335
Ama87I CYCGRG 1 cut(s) 368
Aor51HI AGCGCT 1 cut(s) 382
AoxI GGCC 2 cut(s) 43, 420
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 1 cut(s) 97
AsiGI ACCGGT 1 cut(s) 476
Asp718I GGTACC 1 cut(s) 303
AspLEI GCGC 3 cut(s) 196, 383, 535
AspS9I GGNCC 3 cut(s) 44, 147, 397
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 4 cut(s) 190, 241, 371, 401
AsuII TTCGAA 2 cut(s) 200, 235
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 147, 397
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 2 cut(s) 277, 303
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 370
BccI CCATC 5 cut(s) 57, 175, 242, 304, 349
BceAI ACGGC 5 cut(s) 19, 37, 58, 73, 558
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 2 cut(s) 419, 495
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 371
BfoI RGCGCY 1 cut(s) 384
BfrI CTTAAG 1 cut(s) 340
BisI GCNGC 8 cut(s) 7, 10, 37, 70, 73, 76, 79, 384
BlsI GCNGC 8 cut(s) 8, 11, 38, 71, 74, 77, 80, 385
Bme1390I CCNGG 3 cut(s) 419, 424, 495
Bme18I GGWCC 2 cut(s) 147, 397
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 44, 147, 397
BmiI GGNNCC 2 cut(s) 279, 305
BmrFI CCNGG 3 cut(s) 419, 424, 495
BmsI GCATC 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 516
Bpu14I TTCGAA 2 cut(s) 200, 235
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 476
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse118I RCCGGY 1 cut(s) 476
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 2 cut(s) 419, 495
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 4 cut(s) 68, 133, 253, 360
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 499
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 1 cut(s) 370
Bsh1236I CGCG 3 cut(s) 145, 535, 589
BshFI GGCC 2 cut(s) 45, 422
BshNI GGYRCC 2 cut(s) 277, 303
BshTI ACCGGT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 155, 423, 477
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 2 cut(s) 45, 422
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 368
Bsp119I TTCGAA 2 cut(s) 200, 235
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 151, 327
BspANI GGCC 2 cut(s) 45, 422
BspFNI CGCG 3 cut(s) 145, 535, 589
BspLI GGNNCC 2 cut(s) 279, 305
BspPI GGATC 2 cut(s) 146, 335
BspT104I TTCGAA 2 cut(s) 200, 235
BspT107I GGYRCC 2 cut(s) 277, 303
BspTI CTTAAG 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 2 cut(s) 151, 327
Bst2UI CCWGG 2 cut(s) 419, 495
Bst6I CTCTTC 2 cut(s) 344, 569
BstAFI CTTAAG 1 cut(s) 340
BstBI TTCGAA 2 cut(s) 200, 235
BstC8I GCNNGC 1 cut(s) 209
BstF5I GGATG 4 cut(s) 68, 133, 253, 360
BstFNI CGCG 3 cut(s) 145, 535, 589
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 3 cut(s) 196, 383, 535
BstKTI GATC 2 cut(s) 154, 330
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 2 cut(s) 151, 327
BstMWI GCNNNNNNNGC 5 cut(s) 42, 66, 75, 78, 84
BstNI CCWGG 2 cut(s) 419, 495
BstSCI CCNGG 3 cut(s) 417, 422, 493
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 3 cut(s) 145, 535, 589
BstV1I GCAGC 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 2 cut(s) 45, 422
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 4 cut(s) 68, 133, 253, 360
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 3 cut(s) 196, 383, 535
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 3 cut(s) 44, 147, 397
CpoI CGGWCCG 1 cut(s) 147
Csp6I GTAC 2 cut(s) 304, 314
CspAI ACCGGT 1 cut(s) 476
CspI CGGWCCG 1 cut(s) 147
CviAII CATG 1 cut(s) 300
CviJI RGCY 7 cut(s) 45, 293, 345, 422, 427, 472, 545
CviKI_1 RGCY 7 cut(s) 45, 293, 345, 422, 427, 472, 545
CviQI GTAC 2 cut(s) 304, 314
DpnI GATC 2 cut(s) 153, 329
DpnII GATC 2 cut(s) 151, 327
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 2 cut(s) 344, 569
EarI CTCTTC 2 cut(s) 344, 569
EciI GGCGGA 2 cut(s) 75, 260
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 147, 397
Eco47III AGCGCT 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 417, 493
FaeI CATG 1 cut(s) 303
FaiI YATR 4 cut(s) 120, 284, 301, 325
FatI CATG 1 cut(s) 299
FauI CCCGC 2 cut(s) 17, 80
FblI GTMKAC 3 cut(s) 171, 414, 580
Fnu4HI GCNGC 8 cut(s) 7, 10, 37, 70, 73, 76, 79, 384
FokI GGATG 4 cut(s) 75, 140, 260, 367
Fsp4HI GCNGC 8 cut(s) 7, 10, 37, 70, 73, 76, 79, 384
GlaI GCGC 3 cut(s) 195, 382, 534
GluI GCNGC 8 cut(s) 7, 10, 37, 70, 73, 76, 79, 384
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 45, 422
HapII CCGG 3 cut(s) 155, 423, 477
HhaI GCGC 3 cut(s) 196, 383, 535
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 3 cut(s) 194, 381, 533
HinP1I GCGC 3 cut(s) 194, 381, 533
HincII GTYRAC 1 cut(s) 581
HindII GTYRAC 1 cut(s) 581
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 7 cut(s) 108, 131, 232, 237, 336, 537, 577
HpaII CCGG 3 cut(s) 155, 423, 477
HphI GGTGA 4 cut(s) 190, 241, 371, 401
Hpy166II GTNNAC 4 cut(s) 172, 211, 415, 581
Hpy188I TCNGA 2 cut(s) 113, 151
Hpy188III TCNNGA 4 cut(s) 368, 370, 527, 560
Hpy8I GTNNAC 4 cut(s) 172, 211, 415, 581
Hpy99I CGWCG 5 cut(s) 44, 139, 142, 145, 566
HpyAV CCTTC 2 cut(s) 268, 356
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 66, 75, 78, 84
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 3 cut(s) 194, 381, 533
KpnI GGTACC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 151, 327
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 153, 329
MboI GATC 2 cut(s) 151, 327
MboII GAAGA 4 cut(s) 22, 361, 521, 586
MfeI CAATTG 1 cut(s) 569
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 4 cut(s) 97, 121, 202, 569
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 2 cut(s) 74, 167
MseI TTAA 3 cut(s) 222, 341, 467
MspCI CTTAAG 1 cut(s) 340
MspI CCGG 3 cut(s) 155, 423, 477
MspR9I CCNGG 3 cut(s) 419, 424, 495
MunI CAATTG 1 cut(s) 569
MvaI CCWGG 2 cut(s) 419, 495
MvnI CGCG 3 cut(s) 145, 535, 589
MwoI GCNNNNNNNGC 5 cut(s) 42, 66, 75, 78, 84
NciI CCSGG 1 cut(s) 424
NdeII GATC 2 cut(s) 151, 327
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 2 cut(s) 279, 305
NmuCI GTSAC 1 cut(s) 389
NspV TTCGAA 2 cut(s) 200, 235
PaeR7I CTCGAG 1 cut(s) 368
PcsI WCGNNNNNNNCGW 3 cut(s) 9, 561, 586
PfeI GAWTC 6 cut(s) 108, 131, 232, 237, 336, 537
PflFI GACNNNGTC 1 cut(s) 395
PinAI ACCGGT 1 cut(s) 476
PkrI GCNGC 8 cut(s) 8, 11, 38, 71, 74, 77, 80, 385
PleI GAGTC 1 cut(s) 585
PpsI GAGTC 1 cut(s) 585
PpuMI RGGWCCY 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 417, 493
PspGI CCWGG 2 cut(s) 417, 493
PspN4I GGNNCC 2 cut(s) 279, 305
PspPI GGNCC 3 cut(s) 44, 147, 397
PspPPI RGGWCCY 1 cut(s) 397
PsyI GACNNNGTC 1 cut(s) 395
RsaI GTAC 2 cut(s) 305, 315
RsaNI GTAC 2 cut(s) 304, 314
Rsr2I CGGWCCG 1 cut(s) 147
RsrII CGGWCCG 1 cut(s) 147
SalI GTCGAC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 222, 341, 467
SatI GCNGC 8 cut(s) 7, 10, 37, 70, 73, 76, 79, 384
Sau3AI GATC 2 cut(s) 151, 327
Sau96I GGNCC 3 cut(s) 44, 147, 397
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 3 cut(s) 419, 424, 495
SduI GDGCHC 1 cut(s) 213
SetI ASST 7 cut(s) 180, 279, 367, 399, 414, 420, 474
SfaNI GCATC 1 cut(s) 250
Sfr274I CTCGAG 1 cut(s) 368
SfuI TTCGAA 2 cut(s) 200, 235
SinI GGWCC 2 cut(s) 147, 397
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 3 cut(s) 340, 368, 527
SmoI CTYRAG 3 cut(s) 340, 368, 527
Sse9I AATT 4 cut(s) 97, 121, 202, 569
StyD4I CCNGG 3 cut(s) 417, 422, 493
TaqI TCGA 8 cut(s) 102, 162, 200, 235, 369, 507, 561, 580
TaqII GACCGA 1 cut(s) 512
TasI AATT 4 cut(s) 97, 121, 202, 569
TauI GCSGC 7 cut(s) 9, 12, 39, 72, 75, 78, 81
TfiI GAWTC 6 cut(s) 108, 131, 232, 237, 336, 537
Tru1I TTAA 3 cut(s) 222, 341, 467
Tru9I TTAA 3 cut(s) 222, 341, 467
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 389
Tth111I GACNNNGTC 1 cut(s) 395
Vha464I CTTAAG 1 cut(s) 340
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 147, 397
XapI RAATTY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 368
XmiI GTMKAC 3 cut(s) 171, 414, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.