Rh4CG031500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001308
Physical Location & Seq
Forward (+)
6210058 .. 6214889
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG031500.1

Sequence Viewer

Length: 477 bp
ATGACTCGGCAATCTCTTCCACCCACGGCGTCACTAGCCTCCCCAATCCTCCGGTATCAGATTAAACGTCTCCGCCCTCGGCTTTCCCTTCAGCTTCAGCTTCAGCGACTTGAGCTGTCGGAACTGCCTCCTAAGTCGGTCAGGGCTCGTCGTCTAGTAAAGCTCGACGATCACCGACGGCGGACTGAAATGGAGACCTTACCGACGGCGACGATGGAGTCGAGGCCCGAAGCGAGGCAAAGTGTTGTCACTATTCAAACATTGGAGGCTCCAGTGGCACCTGTAATGGCACTGAGTGGAGTGAGGAGAAATCAAGATGGTTTGGATGACTTATCACCTAAAAAACCTAGGCTGAGTTTGGCGGTTGGCAAGACCAACCTGGATGCTGTGTCGATGGGGCTTGCCTTGGTGTGGCTACTACACAAGAGGTTCCAAAGAAAAGAGGGCGCGCTAGAGGTGCTACAAGAAAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.9

Weight (kDa)

11.02

Isoelectric Point (pI)

72.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 277
AccII CGCG 1 cut(s) 449
AciI CCGC 3 cut(s) 73, 181, 362
AcuI CTGAAG 3 cut(s) 74, 80, 86
AcyI GRCGYC 1 cut(s) 29
AdeI CACNNNGTG 1 cut(s) 296
AfiI CCNNNNNNNGG 2 cut(s) 234, 411
AgsI TTSAA 1 cut(s) 257
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 4 cut(s) 94, 100, 115, 163
AluI AGCT 4 cut(s) 94, 100, 115, 163
Alw26I GTCTC 2 cut(s) 74, 188
AoxI GGCC 1 cut(s) 224
AspA2I CCTAGG 1 cut(s) 347
AspLEI GCGC 2 cut(s) 449, 451
AspS9I GGNCC 1 cut(s) 225
AsuHPI GGTGA 2 cut(s) 164, 327
AvrII CCTAGG 1 cut(s) 347
BanI GGYRCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 148
BccI CCATC 3 cut(s) 208, 311, 388
BceAI ACGGC 3 cut(s) 42, 194, 222
BciT130I CCWGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 74, 188
BfaI CTAG 4 cut(s) 35, 155, 348, 452
BlnI CCTAGG 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 3 cut(s) 270, 279, 431
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 24, 77, 347, 405
BsaWI WCCGGW 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 411
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 4 cut(s) 24, 77, 347, 405
BseGI GGATG 2 cut(s) 331, 388
BseLI CCNNNNNNNGG 2 cut(s) 234, 411
BseMII CTCAG 2 cut(s) 284, 344
BseNI ACTGG 1 cut(s) 272
BsePI GCGCGC 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 1 cut(s) 226
BshNI GGYRCC 1 cut(s) 277
BsiSI CCGG 1 cut(s) 52
BslI CCNNNNNNNGG 2 cut(s) 234, 411
BsmAI GTCTC 2 cut(s) 74, 188
BsmBI CGTCTC 1 cut(s) 74
BsnI GGCC 1 cut(s) 226
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 1 cut(s) 169
BspACI CCGC 3 cut(s) 73, 181, 362
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 2 cut(s) 285, 345
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 3 cut(s) 270, 279, 431
BspT107I GGYRCC 1 cut(s) 277
BspTNI GGTCTC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 4 cut(s) 24, 77, 347, 405
BssHII GCGCGC 1 cut(s) 447
BssMI GATC 1 cut(s) 169
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 347, 405
Bst2UI CCWGG 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 402, 449
BstDEI CTNAG 3 cut(s) 132, 293, 353
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 331, 388
BstFNI CGCG 1 cut(s) 449
BstHHI GCGC 2 cut(s) 449, 451
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 74, 188
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 4 cut(s) 35, 112, 275, 457
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 378
BstUI CGCG 1 cut(s) 449
BsuRI GGCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 331, 388
BtsIMutI CAGTG 2 cut(s) 279, 290
Cac8I GCNNGC 2 cut(s) 402, 449
CfoI GCGC 2 cut(s) 449, 451
Cfr13I GGNCC 1 cut(s) 225
CseI GACGC 1 cut(s) 18
DdeI CTNAG 3 cut(s) 132, 293, 353
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
DraIII CACNNNGTG 1 cut(s) 296
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
EciI GGCGGA 2 cut(s) 62, 196
Eco130I CCWWGG 2 cut(s) 347, 405
Eco24I GRGCYC 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 3 cut(s) 74, 80, 86
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 347, 405
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 347, 405
Esp3I CGTCTC 1 cut(s) 74
FokI GGATG 2 cut(s) 338, 395
FriOI GRGCYC 1 cut(s) 148
FspBI CTAG 4 cut(s) 35, 155, 348, 452
GlaI GCGC 2 cut(s) 448, 450
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 52
HgaI GACGC 1 cut(s) 18
HhaI GCGC 2 cut(s) 449, 451
Hin1I GRCGYC 1 cut(s) 29
Hin6I GCGC 2 cut(s) 447, 449
HinP1I GCGC 2 cut(s) 447, 449
HinfI GANTC 2 cut(s) 4, 218
HpaII CCGG 1 cut(s) 52
HphI GGTGA 2 cut(s) 164, 327
Hpy188I TCNGA 2 cut(s) 60, 121
Hpy188III TCNNGA 1 cut(s) 314
Hpy99I CGWCG 5 cut(s) 153, 170, 180, 208, 214
HpyAV CCTTC 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 112, 275, 457
HpyF3I CTNAG 3 cut(s) 132, 293, 353
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 29
HspAI GCGC 2 cut(s) 447, 449
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 6 cut(s) 65, 127, 285, 294, 365, 392
LweI GCATC 1 cut(s) 373
MaeI CTAG 4 cut(s) 35, 155, 348, 452
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 2 cut(s) 30, 247
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 1 cut(s) 472
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 99
MseI TTAA 1 cut(s) 63
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 380
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 4 cut(s) 35, 112, 275, 457
NdeII GATC 1 cut(s) 169
NlaIV GGNNCC 3 cut(s) 270, 279, 431
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 30, 247
PauI GCGCGC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 3 cut(s) 270, 279, 431
PspPI GGNCC 1 cut(s) 225
PteI GCGCGC 1 cut(s) 447
SaqAI TTAA 1 cut(s) 63
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 1 cut(s) 472
SsiI CCGC 3 cut(s) 73, 181, 362
SspMI CTAG 4 cut(s) 35, 155, 348, 452
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 2 cut(s) 347, 405
TaiI ACGT 1 cut(s) 70
TaqI TCGA 3 cut(s) 165, 221, 392
TaqII GACCGA 1 cut(s) 127
TasI AATT 1 cut(s) 472
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 279, 297
TseFI GTSAC 2 cut(s) 30, 247
Tsp45I GTSAC 2 cut(s) 30, 247
TspRI CASTG 2 cut(s) 279, 297
XmaJI CCTAGG 1 cut(s) 347
XspI CTAG 4 cut(s) 35, 155, 348, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.