AT1G25350

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000872
Physical Location & Seq
Reverse (-)
8889018 .. 8894306
5289 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25350.1

Sequence Viewer

Length: 2388 bp
ATGGTTCTCAAAGACGATAACTCCGAGAAATCTATTGAGCTCTTCATTAGTATTGGTTTGGACGAGAAAACAGCTCGTAATACCATCAATAATAACAAGGTCACCGCCAATCTCACCGCCGTTATCCACGAGGCTGCTGTTACTGATGGGTGCGATCGAAATACTGGCAATCTGTTATACTCGGTCGCTACTAAGTTCCCTACAAATGCTCTTGTGCATCGCCCTACATTGCTCAAGTACATTGTTAACTCTAAGATTAAAACTCCAGCCCAATTGGAAGCTGCATTTGCCTTTTTTGCCAGTACCGGTCCTGAGGACTTTAAGCTGAATGAGTTTGAAGAGGCATGTGGTGTTGGGATTGAAGTTTCCCCAGAAGATATTGAGAAAGCAGTTAAAGGAATCTTTGAAGAGAATAAGAAAACAATATTGGAGCAGCGCTATCGAACTAATGTGGGTGAATTATTTGGACATGTCCGGAAAAGTTTGCCATGGGCCGATCCTAAGATTGTAAAGAAACTCATAGATGAGAAAATGTATGAGCTGCTTGGTGAGAAAACCGCTGCTGATAATGAAAAACCTACAAAAAAGAAGGAGAAGAAGGAGAAGCCTGCCAAAGTCGAGGAAAAGAAAGCTGTGGTGGAAACTACCGCAGAGCCATCTGAAGAGGAGCTTAATCCATATACTATATTCCCTCAGCCAGAACAAAATTTTATGGTTCACACAGAAGTGTTTTTCAGTGATGGCTCTATTCTCAGATGTAGCAATACAAAGGAAGTACTTGACAAACATCTCAAGGTGACTGGAGGGAAAGTTTATACTCGGTTCCCCCCTGAACCAAATGGTTATCTTCATATTGGACATGCCAAGGCTATGTTTGTTGATTTTGGTCTTGCAAAGGAGCGAGGGGGTTGCTGTTATCTAAGGTATGATGATACAAATCCAGAGGCAGAAAAGGAAGAGTATATTAATCATATTGAAGAAATTGTTAAGTGGATGGGTTGGGAACCCTTCAAGATTACATACACCAGTGATTATTTCCAGGAACTGTATGATTTGGCAGTGGAGTTGATTCGGAGAGGTCATGCTTATGTTGATCATCAGACTGCTGATGAGATAAAAGAGTACAGAGAGAAGAAAATGAACAGCCCCTGGAGGGACAGACCTATTGAAGAATCTCTAAAACTTTTTGATGAAATGAGACGAGGCATCATTGAGGAAGGGAAGGCAACCCTAAGAATGAAGCAAGATATGCAGAGTGACAATTTTAATATGTATGATCTTATTGCCTATCGAATAAAGTTCGCACCTCATCCTAAGGCTGGTGACAAGTGGTGTATCTATCCGAGTTATGATTATGCCCACTGCACTGTTGATTCTCTTGAGAATATAACGCATTCGCTCTGTACTCTTGAATTTGAAACCCGGCGTGCTTCATACTATTGGCTGTTACATTCCCTGAGTCTCTACATGCCATATGTGTGGGAATATTCACGGTTGAATGTGACAAACACTGTAATGTCCAAGCGTAAGTTGAATTACATAGTGACAAACAAGTATGTTGATGGTTGGGATGATCCACGTCTTTTGACACTTTCTGGTTTGAGACGGAGGGGTGTGACTTCAACTGCGATTAATGCATTTGTACGAGGCATTGGGATTACTAGAAGTGATGGTAGCATGATACACGTAAGTCGTCTTGAGCATCATATTAGAGAGGAATTGAATAAAACAGCCCCTCGCACCATGGTGGTGCTTAATCCTCTTAAAGTGGTCATCACCAATTTGGAATCAGACAAGCTTATAGAGCTTGATGCCAAAAGGTGGCCTGATGCTCAGAACGATGATCCCTCAGCATTCTACAAGGTACCGTTCTCTAGAGTTGTATACATTGACCAATCTGACTTCCGAATGAAGGATTCAAAAGATTATTATGGGCTCGCCCCTGGTAAATCTGTTTTGCTAAGATATGCTTTCCCAATCAAGTGCACCAATGTTGTCTTTGCTGATGACAATGAAACCGTTCGTGAGATTCATGCGGAATATGACCCCGAGAAAAAGTCAAAGCCAAAGGGGGTTCTACACTGGGTCGCTGAATCTTCCCCAGGAGAAGAGCCCATAAAGGTGGAAGTCCGATTATTTGAGAAACTCTTTAACTCCGAGAACCCGGCTGAACTCAATGATGCTTGGCTCACTGACATTAACCCAAACTCTAAAATGGTAATTTCTGGTGCCTATGCTGTATCAACCCTTAAAGATGCTGCAGTTGGGGACAGATTCCAATTCGAGAGGCTAGGTTATTACGCGGTGGACAAGGACTCTGAGCCAGGAAAGCTTGTGTTCAACCGGACGGTCACACTCAGAGACAGCTACGGGAAAGGTGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

795

Amino Acids

91.25

Weight (kDa)

6.11

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 10 - 167 3.1e-58 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 170 - 248 5.9e-09 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 270 - 575 5.1e-119 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 578 - 682 8.2e-20 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 692 - 770 2.2e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1864
AccB1I GGYRCC 2 cut(s) 1864, 2228
AccB7I CCANNNNNTGG 1 cut(s) 1821
AccI GTMKAC 1 cut(s) 1884
AccII CGCG 1 cut(s) 2303
AccIII TCCGGA 1 cut(s) 474
AciI CCGC 6 cut(s) 105, 117, 558, 648, 2036, 2303
AclWI GGATC 3 cut(s) 491, 1568, 1838
AcsI RAATTY 2 cut(s) 706, 1412
AcuI CTGAAG 1 cut(s) 681
AfaI GTAC 7 cut(s) 239, 304, 777, 1124, 1405, 1644, 1866
AfeI AGCGCT 1 cut(s) 437
AfiI CCNNNNNNNGG 4 cut(s) 1153, 1319, 1821, 1912
AflIII ACRYGT 2 cut(s) 469, 1684
AgeI ACCGGT 1 cut(s) 305
AjiI CACGTC 1 cut(s) 1580
AjnI CCWGG 5 cut(s) 1038, 1148, 1942, 2101, 2323
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AleI CACNNNNGTG 2 cut(s) 725, 1745
Alw21I GWGCWC 2 cut(s) 42, 1988
Alw26I GTCTC 4 cut(s) 1192, 1466, 1597, 2355
Alw44I GTGCAC 1 cut(s) 1984
AlwI GGATC 3 cut(s) 491, 1568, 1838
AlwNI CAGNNNCTG 2 cut(s) 1045, 1149
Ama87I CYCGRG 1 cut(s) 2048
Aor13HI TCCGGA 1 cut(s) 474
Aor51HI AGCGCT 1 cut(s) 437
AoxI GGCC 2 cut(s) 492, 1823
ApaLI GTGCAC 1 cut(s) 1984
ApeKI GCWGC 6 cut(s) 134, 281, 433, 541, 560, 2258
ApoI RAATTY 2 cut(s) 706, 1412
AseI ATTAAT 2 cut(s) 966, 1632
AsiGI ACCGGT 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 2019
Asp718I GGTACC 1 cut(s) 1864
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 308, 492
AsuC2I CCSGG 2 cut(s) 1423, 2165
AsuHPI GGTGA 7 cut(s) 94, 106, 467, 560, 808, 1334, 1768
AvaI CYCGRG 1 cut(s) 2048
AvaII GGWCC 1 cut(s) 308
AxyI CCTNAGG 2 cut(s) 312, 1314
BaeGI GKGCMC 1 cut(s) 1988
BanI GGYRCC 2 cut(s) 1864, 2228
BanII GRGCYC 3 cut(s) 42, 1938, 2115
BauI CACGAG 1 cut(s) 128
Bbv12I GWGCWC 2 cut(s) 42, 1988
BbvCI CCTCAGC 2 cut(s) 693, 1849
BbvI GCAGC 6 cut(s) 121, 268, 445, 528, 547, 2245
BccI CCATC 7 cut(s) 92, 140, 664, 734, 988, 1556, 1664
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 4 cut(s) 422, 456, 891, 925
BciT130I CCWGG 5 cut(s) 1040, 1150, 1944, 2103, 2325
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 2 cut(s) 1423, 2165
BcoDI GTCTC 4 cut(s) 1192, 1466, 1597, 2355
BfaI CTAG 3 cut(s) 1662, 1875, 2291
BfmI CTRYAG 1 cut(s) 2259
BfoI RGCGCY 1 cut(s) 439
BisI GCNGC 6 cut(s) 135, 282, 434, 542, 561, 2259
BlsI GCNGC 6 cut(s) 136, 283, 435, 543, 562, 2260
BmcAI AGTACT 1 cut(s) 777
Bme1390I CCNGG 7 cut(s) 1040, 1150, 1423, 1944, 2103, 2165, 2325
Bme18I GGWCC 1 cut(s) 308
BmeT110I CYCGRG 1 cut(s) 2048
BmgBI CACGTC 1 cut(s) 1580
BmgT120I GGNCC 2 cut(s) 308, 492
BmiI GGNNCC 4 cut(s) 824, 1005, 1866, 2230
BmrFI CCNGG 7 cut(s) 1040, 1150, 1423, 1944, 2103, 2165, 2325
BmrI ACTGGG 1 cut(s) 2092
BmsI GCATC 7 cut(s) 226, 1215, 1711, 1801, 1819, 2170, 2245
BmuI ACTGGG 1 cut(s) 2092
BpmI CTGGAG 3 cut(s) 249, 822, 1171
Bpu10I CCTNAGC 2 cut(s) 693, 1849
BpuEI CTTGAG 4 cut(s) 218, 776, 1400, 1718
BpuMI CCSGG 2 cut(s) 1423, 2165
BsaAI YACGTR 1 cut(s) 1687
BsaBI GATNNNNATC 1 cut(s) 936
BsaJI CCNNGG 6 cut(s) 488, 864, 1148, 1743, 1942, 2101
BsaWI WCCGGW 3 cut(s) 305, 474, 2343
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 795, 825
Bsc4I CCNNNNNNNGG 4 cut(s) 1153, 1319, 1821, 1912
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 5 cut(s) 169, 300, 805, 1026, 2087
Bse21I CCTNAGG 2 cut(s) 312, 1314
Bse3DI GCAATG 1 cut(s) 227
Bse8I GATNNNNATC 1 cut(s) 936
BseAI TCCGGA 1 cut(s) 474
BseBI CCWGG 5 cut(s) 1040, 1150, 1944, 2103, 2325
BseDI CCNNGG 6 cut(s) 488, 864, 1148, 1743, 1942, 2101
BseGI GGATG 3 cut(s) 999, 1309, 1576
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 4 cut(s) 1153, 1319, 1821, 1912
BseMI GCAATG 1 cut(s) 227
BseMII CTCAG 8 cut(s) 303, 707, 766, 1448, 1847, 1863, 2310, 2371
BseNI ACTGG 5 cut(s) 169, 300, 805, 1026, 2087
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 1988
BseXI GCAGC 6 cut(s) 121, 268, 445, 528, 547, 2245
BsgI GTGCAG 1 cut(s) 1348
Bsh1236I CGCG 1 cut(s) 2303
Bsh1285I CGRYCG 2 cut(s) 157, 186
BshFI GGCC 2 cut(s) 494, 1825
BshNI GGYRCC 2 cut(s) 1864, 2228
BshTI ACCGGT 1 cut(s) 305
BsiEI CGRYCG 2 cut(s) 157, 186
BsiHKAI GWGCWC 2 cut(s) 42, 1988
BsiHKCI CYCGRG 1 cut(s) 2048
BsiSI CCGG 5 cut(s) 306, 475, 1423, 2165, 2344
BslFI GGGAC 2 cut(s) 1169, 2282
BslI CCNNNNNNNGG 4 cut(s) 1153, 1319, 1821, 1912
BsmAI GTCTC 4 cut(s) 1192, 1466, 1597, 2355
BsmBI CGTCTC 2 cut(s) 1192, 1597
BsmFI GGGAC 2 cut(s) 1169, 2282
BsmI GAATGC 2 cut(s) 1393, 1853
BsnI GGCC 2 cut(s) 494, 1825
BsoBI CYCGRG 1 cut(s) 2048
Bsp1286I GDGCHC 4 cut(s) 42, 1938, 1988, 2115
Bsp13I TCCGGA 1 cut(s) 474
Bsp143I GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
Bsp19I CCATGG 2 cut(s) 488, 1743
BspACI CCGC 6 cut(s) 105, 117, 558, 648, 2036, 2303
BspANI GGCC 2 cut(s) 494, 1825
BspCNI CTCAG 8 cut(s) 304, 706, 765, 1449, 1846, 1862, 2311, 2370
BspEI TCCGGA 1 cut(s) 474
BspFNI CGCG 1 cut(s) 2303
BspLI GGNNCC 4 cut(s) 824, 1005, 1866, 2230
BspMAI CTGCAG 1 cut(s) 2263
BspPI GGATC 3 cut(s) 491, 1568, 1838
BspQI GCTCTTC 2 cut(s) 47, 2103
BspT107I GGYRCC 2 cut(s) 1864, 2228
BsrDI GCAATG 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 5 cut(s) 169, 300, 805, 1026, 2087
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 6 cut(s) 488, 864, 1148, 1743, 1942, 2101
BssMI GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
BssNAI GTATAC 1 cut(s) 1885
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 3 cut(s) 488, 864, 1743
Bst1107I GTATAC 1 cut(s) 1885
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 5 cut(s) 1040, 1150, 1944, 2103, 2325
Bst4CI ACNGT 7 cut(s) 1047, 1369, 1494, 1513, 1869, 2020, 2350
Bst6I CTCTTC 6 cut(s) 47, 333, 402, 657, 951, 2103
BstAPI GCANNNNNTGC 1 cut(s) 1249
BstBAI YACGTR 1 cut(s) 1687
BstC8I GCNNGC 3 cut(s) 609, 1428, 1938
BstDSI CCRYGG 2 cut(s) 488, 1743
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 3 cut(s) 999, 1309, 1576
BstFNI CGCG 1 cut(s) 2303
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 6 cut(s) 157, 499, 1096, 1279, 1576, 1846
BstMAI GTCTC 4 cut(s) 1192, 1466, 1597, 2355
BstMBI GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
BstMCI CGRYCG 2 cut(s) 157, 186
BstMWI GCNNNNNNNGC 6 cut(s) 287, 296, 1249, 1634, 1804, 2329
BstNI CCWGG 5 cut(s) 1040, 1150, 1944, 2103, 2325
BstNSI RCATGY 4 cut(s) 348, 473, 863, 1471
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 7 cut(s) 1038, 1148, 1421, 1942, 2101, 2163, 2323
BstSFI CTRYAG 1 cut(s) 2259
BstSLI GKGCMC 1 cut(s) 1988
BstUI CGCG 1 cut(s) 2303
BstV1I GCAGC 6 cut(s) 121, 268, 445, 528, 547, 2245
BstXI CCANNNNNNTGG 2 cut(s) 1479, 2122
BstZ17I GTATAC 1 cut(s) 1885
Bsu36I CCTNAGG 2 cut(s) 312, 1314
BsuRI GGCC 2 cut(s) 494, 1825
BtgI CCRYGG 2 cut(s) 488, 1743
BtgZI GCGATG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 1580
BtsCI GGATG 3 cut(s) 999, 1309, 1576
BtsI GCAGTG 2 cut(s) 1065, 1360
BtsIMutI CAGTG 8 cut(s) 742, 1033, 1065, 1360, 1365, 1509, 2080, 2190
Cac8I GCNNGC 3 cut(s) 609, 1428, 1938
CaiI CAGNNNCTG 2 cut(s) 1045, 1149
CfoI GCGC 1 cut(s) 438
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 2 cut(s) 308, 492
Csp6I GTAC 7 cut(s) 238, 303, 776, 1123, 1404, 1643, 1865
CspAI ACCGGT 1 cut(s) 305
CspCI CAANNNNNGTGG 2 cut(s) 1566, 1601
CviAII CATG 9 cut(s) 345, 470, 489, 860, 1082, 1468, 1678, 1744, 2033
CviQI GTAC 7 cut(s) 238, 303, 776, 1123, 1404, 1643, 1865
DpnI GATC 6 cut(s) 156, 498, 1095, 1278, 1575, 1845
DpnII GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
Eam1104I CTCTTC 6 cut(s) 47, 333, 402, 657, 951, 2103
EarI CTCTTC 6 cut(s) 47, 333, 402, 657, 951, 2103
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 3 cut(s) 488, 864, 1743
Eco24I GRGCYC 3 cut(s) 42, 1938, 2115
Eco47I GGWCC 1 cut(s) 308
Eco47III AGCGCT 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 681
Eco81I CCTNAGG 2 cut(s) 312, 1314
Eco88I CYCGRG 1 cut(s) 2048
Eco91I GGTNACC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 5 cut(s) 1038, 1148, 1942, 2101, 2323
EcoT14I CCWWGG 3 cut(s) 488, 864, 1743
EcoT22I ATGCAT 1 cut(s) 1639
EcoT38I GRGCYC 3 cut(s) 42, 1938, 2115
ErhI CCWWGG 3 cut(s) 488, 864, 1743
Esp3I CGTCTC 2 cut(s) 1192, 1597
FaeI CATG 9 cut(s) 348, 473, 492, 863, 1085, 1471, 1681, 1747, 2036
FalI AAGNNNNNCTT 4 cut(s) 654, 686, 1954, 1986
FaqI GGGAC 2 cut(s) 1169, 2282
FatI CATG 9 cut(s) 344, 469, 488, 859, 1081, 1467, 1677, 1743, 2032
FauNDI CATATG 1 cut(s) 1474
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 1 cut(s) 1884
Fnu4HI GCNGC 6 cut(s) 135, 282, 434, 542, 561, 2259
FokI GGATG 3 cut(s) 1006, 1296, 1583
FriOI GRGCYC 3 cut(s) 42, 1938, 2115
Fsp4HI GCNGC 6 cut(s) 135, 282, 434, 542, 561, 2259
FspBI CTAG 3 cut(s) 1662, 1875, 2291
GlaI GCGC 1 cut(s) 437
GluI GCNGC 6 cut(s) 135, 282, 434, 542, 561, 2259
GsuI CTGGAG 3 cut(s) 249, 822, 1171
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 2 cut(s) 494, 1825
HapII CCGG 5 cut(s) 306, 475, 1423, 2165, 2344
HhaI GCGC 1 cut(s) 438
Hin1II CATG 9 cut(s) 348, 473, 492, 863, 1085, 1471, 1681, 1747, 2036
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 2 cut(s) 1796, 2330
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 5 cut(s) 306, 475, 1423, 2165, 2344
HphI GGTGA 7 cut(s) 94, 106, 467, 560, 808, 1334, 1768
Hpy166II GTNNAC 5 cut(s) 247, 718, 1885, 1986, 2308
Hpy8I GTNNAC 5 cut(s) 247, 718, 1885, 1986, 2308
HpyAV CCTTC 6 cut(s) 583, 592, 1018, 1211, 1216, 1906
HpyCH4III ACNGT 7 cut(s) 1047, 1369, 1494, 1513, 1869, 2020, 2350
HpyCH4IV ACGT 2 cut(s) 1579, 1686
HpyCH4V TGCA 8 cut(s) 217, 284, 893, 1252, 1365, 1637, 1986, 2261
HpyF10VI GCNNNNNNNGC 6 cut(s) 287, 296, 1249, 1634, 1804, 2329
HpySE526I ACGT 2 cut(s) 1579, 1686
Hsp92II CATG 9 cut(s) 348, 473, 492, 863, 1085, 1471, 1681, 1747, 2036
HspAI GCGC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 474
KpnI GGTACC 1 cut(s) 1868
Ksp22I TGATCA 1 cut(s) 1093
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
LguI GCTCTTC 2 cut(s) 47, 2103
LmnI GCTCC 3 cut(s) 430, 667, 898
Lsp1109I GCAGC 6 cut(s) 121, 268, 445, 528, 547, 2245
LweI GCATC 7 cut(s) 226, 1215, 1711, 1801, 1819, 2170, 2245
MaeI CTAG 3 cut(s) 1662, 1875, 2291
MaeII ACGT 2 cut(s) 1579, 1686
MalI GATC 6 cut(s) 156, 498, 1095, 1278, 1575, 1845
MboI GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
MfeI CAATTG 1 cut(s) 272
MhlI GDGCHC 4 cut(s) 42, 1938, 1988, 2115
MlyI GAGTC 2 cut(s) 1468, 2309
Mph1103I ATGCAT 1 cut(s) 1639
MroI TCCGGA 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 2019
MslI CAYNNNNRTG 7 cut(s) 725, 1086, 1477, 1514, 1745, 1748, 2120
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 5 cut(s) 306, 475, 1423, 2165, 2344
MspR9I CCNGG 7 cut(s) 1040, 1150, 1423, 1944, 2103, 2165, 2325
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 2 cut(s) 1393, 1853
MvaI CCWGG 5 cut(s) 1040, 1150, 1944, 2103, 2325
MvnI CGCG 1 cut(s) 2303
MwoI GCNNNNNNNGC 6 cut(s) 287, 296, 1249, 1634, 1804, 2329
NciI CCSGG 2 cut(s) 1423, 2165
NcoI CCATGG 2 cut(s) 488, 1743
NdeI CATATG 1 cut(s) 1474
NdeII GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
NlaIII CATG 9 cut(s) 348, 473, 492, 863, 1085, 1471, 1681, 1747, 2036
NlaIV GGNNCC 4 cut(s) 824, 1005, 1866, 2230
NmuCI GTSAC 8 cut(s) 100, 796, 1256, 1322, 1501, 1543, 1615, 2350
NsiI ATGCAT 1 cut(s) 1639
NspI RCATGY 4 cut(s) 348, 473, 863, 1471
OliI CACNNNNGTG 2 cut(s) 725, 1745
PciI ACATGT 1 cut(s) 469
PciSI GCTCTTC 2 cut(s) 47, 2103
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 2 cut(s) 1393, 1853
PdmI GAANNNNTTC 1 cut(s) 2019
PfeI GAWTC 9 cut(s) 399, 1069, 1172, 1373, 1787, 1916, 2029, 2093, 2274
PflMI CCANNNNNTGG 1 cut(s) 1821
PfoI TCCNGGA 1 cut(s) 1038
PinAI ACCGGT 1 cut(s) 305
PkrI GCNGC 6 cut(s) 136, 283, 435, 543, 562, 2260
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 2 cut(s) 1467, 2309
PpsI GAGTC 2 cut(s) 1467, 2309
Ppu21I YACGTR 1 cut(s) 1687
PscI ACATGT 1 cut(s) 469
PshBI ATTAAT 2 cut(s) 966, 1632
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 5 cut(s) 1038, 1148, 1942, 2101, 2323
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 5 cut(s) 1038, 1148, 1942, 2101, 2323
PspN4I GGNNCC 4 cut(s) 824, 1005, 1866, 2230
PspPI GGNCC 2 cut(s) 308, 492
PstI CTGCAG 1 cut(s) 2263
PstNI CAGNNNCTG 2 cut(s) 1045, 1149
PvuI CGATCG 1 cut(s) 157
RsaI GTAC 7 cut(s) 239, 304, 777, 1124, 1405, 1644, 1866
RsaNI GTAC 7 cut(s) 238, 303, 776, 1123, 1404, 1643, 1865
RseI CAYNNNNRTG 7 cut(s) 725, 1086, 1477, 1514, 1745, 1748, 2120
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 2 cut(s) 47, 2103
SatI GCNGC 6 cut(s) 135, 282, 434, 542, 561, 2259
Sau3AI GATC 6 cut(s) 154, 496, 1093, 1276, 1573, 1843
Sau96I GGNCC 2 cut(s) 308, 492
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 2 cut(s) 1468, 2309
ScrFI CCNGG 7 cut(s) 1040, 1150, 1423, 1944, 2103, 2165, 2325
SduI GDGCHC 4 cut(s) 42, 1938, 1988, 2115
SfaNI GCATC 7 cut(s) 226, 1215, 1711, 1801, 1819, 2170, 2245
SfcI CTRYAG 1 cut(s) 2259
SinI GGWCC 1 cut(s) 308
SmiMI CAYNNNNRTG 7 cut(s) 725, 1086, 1477, 1514, 1745, 1748, 2120
SmlI CTYRAG 4 cut(s) 233, 791, 1379, 1697
SmoI CTYRAG 4 cut(s) 233, 791, 1379, 1697
SsiI CCGC 6 cut(s) 105, 117, 558, 648, 2036, 2303
SspI AATATT 2 cut(s) 426, 1487
SspMI CTAG 3 cut(s) 1662, 1875, 2291
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 7 cut(s) 1038, 1148, 1421, 1942, 2101, 2163, 2323
StyI CCWWGG 3 cut(s) 488, 864, 1743
TaaI ACNGT 7 cut(s) 1047, 1369, 1494, 1513, 1869, 2020, 2350
TaiI ACGT 2 cut(s) 1582, 1689
TaqI TCGA 5 cut(s) 157, 442, 618, 1291, 2283
TaqII GACCGA 1 cut(s) 172
TatI WGTACW 4 cut(s) 237, 775, 1122, 1403
TfiI GAWTC 9 cut(s) 399, 1069, 1172, 1373, 1787, 1916, 2029, 2093, 2274
TscAI CASTG 8 cut(s) 742, 1033, 1065, 1367, 1372, 1516, 2087, 2197
TseFI GTSAC 8 cut(s) 100, 796, 1256, 1322, 1501, 1543, 1615, 2350
TseI GCWGC 6 cut(s) 134, 281, 433, 541, 560, 2258
Tsp45I GTSAC 8 cut(s) 100, 796, 1256, 1322, 1501, 1543, 1615, 2350
TspGWI ACGGA 1 cut(s) 1621
TspRI CASTG 8 cut(s) 742, 1033, 1065, 1367, 1372, 1516, 2087, 2197
Van91I CCANNNNNTGG 1 cut(s) 1821
VneI GTGCAC 1 cut(s) 1984
VpaK11BI GGWCC 1 cut(s) 308
VspI ATTAAT 2 cut(s) 966, 1632
XapI RAATTY 2 cut(s) 706, 1412
XbaI TCTAGA 1 cut(s) 1874
XceI RCATGY 4 cut(s) 348, 473, 863, 1471
XmiI GTMKAC 1 cut(s) 1884
XmnI GAANNNNTTC 1 cut(s) 2019
XspI CTAG 3 cut(s) 1662, 1875, 2291
ZrmI AGTACT 1 cut(s) 777
Zsp2I ATGCAT 1 cut(s) 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.