Rroxscaffold_159G00433020

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0000872
Physical Location & Seq
Forward (+)
758191 .. 761137
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00433020.1

Sequence Viewer

Length: 1476 bp
ATGGATGCAGTCGATCGAAGCATTGGTAATCTTATATACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCTTCGCCCGCTATTGGCACAGTACATTGTGTCTTCAAAGATTAAAACTCCAGCTCAGTTAGATGCGGCTTTGTTATTTTTTGCAAATACGGGGCCAGGAAACTTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGGCTTATTTGCACATCTTAGGAACAGACATCCGTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAAGTTGGGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAACAAGAAAGAGAAACCTGCTAAAGTAGAGGAGAAAGCTACTGCTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGATGTTGCAACTCAAAGGAACTCCTTCAAAAACACTTGAGCTTGACTGGGGGAGAGTTCTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGACTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAAGAATACATTGATCATATTCAAGAGATTGTCAGTTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTTCACGATCTGTATGAATTAGCATTGGAGCTCATACGAAGTGGTCAAGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGGGAAAAAAATGAACAGCCCTTGGAGGGACAGGGCAATTAAAGAGTCGCTGACACTTTTTGAGGATACGAGGCGTGGCCTGATTGAAGAAGGGAAAGCAACTCTGAGAATGAAACAAGACATGCAGAGTGATAATTTTAACATAGTCGGCGAAAACTCGGGGCCGGCCACGATAGTGGATTCTCTTGAGAATATCACTCATTCGCTGTGCACTCTAGAATTTGAGACACGGCGTGCTTCATACTACCGGTTATTGCATTCTCCGGGCCTATATCAACCACATGTTGGGAATATTCAAGACCGAATGTCGAACACCGTTATGTCGAAGCGTCGTCATTACCAACCGGAAAGATGGCCTCATAAAGGACTCGTGAAGCAAGAAAGTGGCCCGATTCGAGGCGAGTCCTACAAGGTTCCAATTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCACCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTCTGCACCGCTCGTATACGCATGTCCTATCAAGTGTGTGGATGCAAAAGATGTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

491

Amino Acids

56.22

Weight (kDa)

6.46

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 4 - 120 6.1e-38 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA-synt_1c PF00749 200 - 349 4.3e-58 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c PF00749 362 - 417 3.8e-09 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 469
AccB7I CCANNNNNTGG 1 cut(s) 1199
AccBSI CCGCTC 1 cut(s) 1425
AccI GTMKAC 1 cut(s) 1430
AccIII TCCGGA 1 cut(s) 727
AciI CCGC 3 cut(s) 83, 140, 1423
AcoI YGGCCR 1 cut(s) 1080
AcsI RAATTY 3 cut(s) 191, 495, 1133
AcuI CTGAAG 1 cut(s) 510
AdeI CACNNNGTG 1 cut(s) 1148
AfaI GTAC 3 cut(s) 98, 911, 1349
AfeI AGCGCT 2 cut(s) 296, 1358
AfiI CCNNNNNNNGG 6 cut(s) 88, 652, 940, 1199, 1277, 1310
AflIII ACRYGT 1 cut(s) 1195
AgeI ACCGGT 1 cut(s) 1161
AgsI TTSAA 9 cut(s) 111, 181, 197, 221, 266, 570, 764, 1001, 1211
AhdI GACNNNNNGTC 1 cut(s) 1219
AhlI ACTAGT 1 cut(s) 812
AjnI CCWGG 3 cut(s) 169, 675, 1405
AleI CACNNNNGTG 1 cut(s) 1088
AluBI AGCT 6 cut(s) 128, 482, 583, 734, 853, 872
AluI AGCT 6 cut(s) 128, 482, 583, 734, 853, 872
Alw21I GWGCWC 2 cut(s) 855, 1127
Alw26I GTCTC 1 cut(s) 1133
Alw44I GTGCAC 1 cut(s) 1123
Ama87I CYCGRG 1 cut(s) 1072
Aor13HI TCCGGA 1 cut(s) 727
Aor51HI AGCGCT 2 cut(s) 296, 1358
AoxI GGCC 9 cut(s) 167, 643, 673, 991, 1076, 1080, 1180, 1268, 1300
ApaLI GTGCAC 1 cut(s) 1123
ApeKI GCWGC 2 cut(s) 292, 419
ApoI RAATTY 3 cut(s) 191, 495, 1133
AsiGI ACCGGT 1 cut(s) 1161
Asp700I GAANNNNTTC 3 cut(s) 435, 495, 565
AspLEI GCGC 2 cut(s) 297, 1359
AspS9I GGNCC 4 cut(s) 167, 1076, 1180, 1301
AsuC2I CCSGG 1 cut(s) 1179
AsuHPI GGTGA 4 cut(s) 407, 419, 707, 1361
AvaI CYCGRG 1 cut(s) 1072
BaeGI GKGCMC 1 cut(s) 1127
BanII GRGCYC 2 cut(s) 795, 855
BauI CACGAG 1 cut(s) 1283
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 855, 1127
BbvI GCAGC 2 cut(s) 304, 431
BccI CCATC 2 cut(s) 775, 1260
BceAI ACGGC 1 cut(s) 1160
BciT130I CCWGG 3 cut(s) 171, 677, 1407
BciVI GTATCC 1 cut(s) 973
BclI TGATCA 2 cut(s) 754, 880
BcnI CCSGG 1 cut(s) 1179
BcoDI GTCTC 1 cut(s) 1133
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 2 cut(s) 813, 1130
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 2 cut(s) 298, 1360
BfuAI ACCTGC 1 cut(s) 469
BfuI GTATCC 1 cut(s) 973
BisI GCNGC 3 cut(s) 141, 293, 420
BlsI GCNGC 3 cut(s) 142, 294, 421
Bme1390I CCNGG 4 cut(s) 171, 677, 1179, 1407
BmeRI GACNNNNNGTC 1 cut(s) 1219
BmeT110I CYCGRG 1 cut(s) 1072
BmgT120I GGNCC 4 cut(s) 167, 1076, 1180, 1301
BmiI GGNNCC 5 cut(s) 168, 438, 792, 1077, 1329
BmrFI CCNGG 4 cut(s) 171, 677, 1179, 1407
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 3 cut(s) 127, 383, 1446
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 108
BpuEI CTTGAG 2 cut(s) 599, 1121
BpuMI CCSGG 1 cut(s) 1179
BsaJI CCNNGG 4 cut(s) 347, 651, 935, 1338
BsaWI WCCGGW 3 cut(s) 727, 1161, 1258
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 652, 940, 1199, 1277, 1310
Bse118I RCCGGY 2 cut(s) 1078, 1161
Bse1I ACTGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 663
BseAI TCCGGA 1 cut(s) 727
BseBI CCWGG 3 cut(s) 171, 677, 1407
BseDI CCNNGG 4 cut(s) 347, 651, 935, 1338
BseGI GGATG 6 cut(s) 10, 343, 374, 786, 901, 1461
BseLI CCNNNNNNNGG 6 cut(s) 88, 652, 940, 1199, 1277, 1310
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 3 cut(s) 143, 698, 1010
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 1127
BseXI GCAGC 2 cut(s) 304, 431
BsgI GTGCAG 1 cut(s) 1403
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 9 cut(s) 169, 645, 675, 993, 1078, 1082, 1182, 1270, 1302
BshTI ACCGGT 1 cut(s) 1161
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 855, 1127
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 5 cut(s) 728, 1079, 1162, 1178, 1259
BslFI GGGAC 1 cut(s) 956
BslI CCNNNNNNNGG 6 cut(s) 88, 652, 940, 1199, 1277, 1310
BsmAI GTCTC 1 cut(s) 1133
BsmFI GGGAC 1 cut(s) 956
BsmI GAATGC 1 cut(s) 1171
BsnI GGCC 9 cut(s) 169, 645, 675, 993, 1078, 1082, 1182, 1270, 1302
BsoBI CYCGRG 1 cut(s) 1072
Bsp1286I GDGCHC 3 cut(s) 795, 855, 1127
Bsp13I TCCGGA 1 cut(s) 727
Bsp1407I TGTACA 1 cut(s) 1347
Bsp143I GATC 5 cut(s) 13, 754, 801, 829, 880
BspACI CCGC 3 cut(s) 83, 140, 1423
BspANI GGCC 9 cut(s) 169, 645, 675, 993, 1078, 1082, 1182, 1270, 1302
BspCNI CTCAG 3 cut(s) 142, 699, 1011
BspEI TCCGGA 1 cut(s) 727
BspLI GGNNCC 5 cut(s) 168, 438, 792, 1077, 1329
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 469
BsrBI CCGCTC 1 cut(s) 1425
BsrDI GCAATG 1 cut(s) 663
BsrFI RCCGGY 2 cut(s) 1078, 1161
BsrGI TGTACA 1 cut(s) 1347
BsrI ACTGG 1 cut(s) 593
BssAI RCCGGY 2 cut(s) 1078, 1161
BssECI CCNNGG 4 cut(s) 347, 651, 935, 1338
BssMI GATC 5 cut(s) 13, 754, 801, 829, 880
BssNAI GTATAC 1 cut(s) 1431
BssSI CACGAG 1 cut(s) 1283
BssT1I CCWWGG 3 cut(s) 651, 935, 1338
Bst1107I GTATAC 1 cut(s) 1431
Bst2BI CACGAG 1 cut(s) 1283
Bst2UI CCWGG 3 cut(s) 171, 677, 1407
Bst4CI ACNGT 3 cut(s) 43, 96, 1231
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 1347
BstC8I GCNNGC 3 cut(s) 83, 1080, 1149
BstDEI CTNAG 4 cut(s) 129, 332, 707, 1019
BstDSI CCRYGG 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 1275
BstF5I GGATG 6 cut(s) 10, 343, 374, 786, 901, 1461
BstH2I RGCGCY 2 cut(s) 298, 1360
BstHHI GCGC 2 cut(s) 297, 1359
BstKTI GATC 5 cut(s) 16, 757, 804, 832, 883
BstMAI GTCTC 1 cut(s) 1133
BstMBI GATC 5 cut(s) 13, 754, 801, 829, 880
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 3 cut(s) 171, 677, 1407
BstNSI RCATGY 4 cut(s) 207, 1039, 1199, 1439
BstSCI CCNGG 4 cut(s) 169, 675, 1177, 1405
BstSFI CTRYAG 1 cut(s) 417
BstSLI GKGCMC 1 cut(s) 1127
BstV1I GCAGC 2 cut(s) 304, 431
BstV2I GAAGAC 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstZ17I GTATAC 1 cut(s) 1431
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 9 cut(s) 169, 645, 675, 993, 1078, 1082, 1182, 1270, 1302
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 6 cut(s) 10, 343, 374, 786, 901, 1461
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 3 cut(s) 83, 1080, 1149
CfoI GCGC 2 cut(s) 297, 1359
Cfr10I RCCGGY 2 cut(s) 1078, 1161
Cfr13I GGNCC 4 cut(s) 167, 1076, 1180, 1301
CseI GACGC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 97, 910, 1348
CspAI ACCGGT 1 cut(s) 1161
CviAII CATG 5 cut(s) 204, 647, 1036, 1196, 1436
CviQI GTAC 3 cut(s) 97, 910, 1348
DdeI CTNAG 4 cut(s) 129, 332, 707, 1019
DpnI GATC 5 cut(s) 15, 756, 803, 831, 882
DpnII GATC 5 cut(s) 13, 754, 801, 829, 880
DraIII CACNNNGTG 1 cut(s) 1148
DriI GACNNNNNGTC 1 cut(s) 1219
EaeI YGGCCR 1 cut(s) 1080
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 1219
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 3 cut(s) 651, 935, 1338
Eco24I GRGCYC 2 cut(s) 795, 855
Eco32I GATATC 1 cut(s) 632
Eco47III AGCGCT 2 cut(s) 296, 1358
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 1072
EcoICRI GAGCTC 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 1275
EcoRII CCWGG 3 cut(s) 169, 675, 1405
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 3 cut(s) 651, 935, 1338
EcoT38I GRGCYC 2 cut(s) 795, 855
ErhI CCWWGG 3 cut(s) 651, 935, 1338
FaeI CATG 5 cut(s) 207, 650, 1039, 1199, 1439
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 1 cut(s) 956
FatI CATG 5 cut(s) 203, 646, 1035, 1195, 1435
FauI CCCGC 1 cut(s) 90
FauNDI CATATG 1 cut(s) 508
FbaI TGATCA 2 cut(s) 754, 880
FblI GTMKAC 1 cut(s) 1430
Fnu4HI GCNGC 3 cut(s) 141, 293, 420
FokI GGATG 6 cut(s) 17, 330, 361, 793, 908, 1468
FriOI GRGCYC 2 cut(s) 795, 855
FseI GGCCGGCC 1 cut(s) 1082
Fsp4HI GCNGC 3 cut(s) 141, 293, 420
FspBI CTAG 2 cut(s) 813, 1130
GlaI GCGC 2 cut(s) 296, 1358
GluI GCNGC 3 cut(s) 141, 293, 420
GsuI CTGGAG 1 cut(s) 108
HaeII RGCGCY 2 cut(s) 298, 1360
HaeIII GGCC 9 cut(s) 169, 645, 675, 993, 1078, 1082, 1182, 1270, 1302
HapII CCGG 5 cut(s) 728, 1079, 1162, 1178, 1259
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 2 cut(s) 297, 1359
Hin1II CATG 5 cut(s) 207, 650, 1039, 1199, 1439
Hin6I GCGC 2 cut(s) 295, 1357
HinP1I GCGC 2 cut(s) 295, 1357
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 6 cut(s) 959, 1094, 1281, 1306, 1316, 1379
HpaII CCGG 5 cut(s) 728, 1079, 1162, 1178, 1259
HphI GGTGA 4 cut(s) 407, 419, 707, 1361
Hpy166II GTNNAC 2 cut(s) 1125, 1431
Hpy188I TCNGA 6 cut(s) 229, 708, 888, 1020, 1363, 1397
Hpy188III TCNNGA 8 cut(s) 617, 728, 764, 827, 1100, 1130, 1211, 1285
Hpy8I GTNNAC 2 cut(s) 1125, 1431
Hpy99I CGWCG 1 cut(s) 1248
HpyAV CCTTC 2 cut(s) 576, 998
HpyCH4III ACNGT 3 cut(s) 43, 96, 1231
HpyCH4IV ACGT 1 cut(s) 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 4 cut(s) 129, 332, 707, 1019
HpySE526I ACGT 1 cut(s) 876
Hsp92II CATG 5 cut(s) 207, 650, 1039, 1199, 1439
HspAI GCGC 2 cut(s) 295, 1357
Kpn2I TCCGGA 1 cut(s) 727
KroI GCCGGC 1 cut(s) 1078
KroNI GCCGGC 1 cut(s) 1080
Ksp22I TGATCA 2 cut(s) 754, 880
Kzo9I GATC 5 cut(s) 13, 754, 801, 829, 880
LmnI GCTCC 5 cut(s) 289, 790, 850, 1364, 1408
Lsp1109I GCAGC 2 cut(s) 304, 431
LweI GCATC 3 cut(s) 127, 383, 1446
MaeI CTAG 2 cut(s) 813, 1130
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 1 cut(s) 695
MalI GATC 5 cut(s) 15, 756, 803, 831, 882
MbiI CCGCTC 1 cut(s) 1425
MboI GATC 5 cut(s) 13, 754, 801, 829, 880
MboII GAAGA 7 cut(s) 99, 209, 242, 245, 278, 503, 1013
MhlI GDGCHC 3 cut(s) 795, 855, 1127
MlyI GAGTC 4 cut(s) 968, 1275, 1325, 1373
MmeI TCCRAC 1 cut(s) 758
MroI TCCGGA 1 cut(s) 727
MroNI GCCGGC 1 cut(s) 1078
MroXI GAANNNNTTC 3 cut(s) 435, 495, 565
MseI TTAA 7 cut(s) 117, 252, 501, 540, 954, 1053, 1374
MslI CAYNNNNRTG 2 cut(s) 1088, 1232
MspI CCGG 5 cut(s) 728, 1079, 1162, 1178, 1259
MspR9I CCNGG 4 cut(s) 171, 677, 1179, 1407
Mva1269I GAATGC 1 cut(s) 1171
MvaI CCWGG 3 cut(s) 171, 677, 1407
MwoI GCNNNNNNNGC 1 cut(s) 82
NaeI GCCGGC 1 cut(s) 1080
NciI CCSGG 1 cut(s) 1179
NdeI CATATG 1 cut(s) 508
NdeII GATC 5 cut(s) 13, 754, 801, 829, 880
NgoMIV GCCGGC 1 cut(s) 1078
NlaIII CATG 5 cut(s) 207, 650, 1039, 1199, 1439
NlaIV GGNNCC 5 cut(s) 168, 438, 792, 1077, 1329
NmuCI GTSAC 1 cut(s) 695
NspI RCATGY 4 cut(s) 207, 1039, 1199, 1439
OliI CACNNNNGTG 1 cut(s) 1088
PciI ACATGT 1 cut(s) 1195
PctI GAATGC 1 cut(s) 1171
PdiI GCCGGC 1 cut(s) 1080
PdmI GAANNNNTTC 3 cut(s) 435, 495, 565
PfeI GAWTC 2 cut(s) 1094, 1306
PflMI CCANNNNNTGG 1 cut(s) 1199
PfoI TCCNGGA 1 cut(s) 1405
PinAI ACCGGT 1 cut(s) 1161
PkrI GCNGC 3 cut(s) 142, 294, 421
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 4 cut(s) 967, 1275, 1324, 1373
PpsI GAGTC 4 cut(s) 967, 1275, 1324, 1373
PscI ACATGT 1 cut(s) 1195
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 169, 675, 1405
PspGI CCWGG 3 cut(s) 169, 675, 1405
PspN4I GGNNCC 5 cut(s) 168, 438, 792, 1077, 1329
PspPI GGNCC 4 cut(s) 167, 1076, 1180, 1301
PstI CTGCAG 1 cut(s) 421
PvuI CGATCG 1 cut(s) 16
RigI GGCCGGCC 1 cut(s) 1082
RsaI GTAC 3 cut(s) 98, 911, 1349
RsaNI GTAC 3 cut(s) 97, 910, 1348
RseI CAYNNNNRTG 2 cut(s) 1088, 1232
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 7 cut(s) 117, 252, 501, 540, 954, 1053, 1374
SatI GCNGC 3 cut(s) 141, 293, 420
Sau3AI GATC 5 cut(s) 13, 754, 801, 829, 880
Sau96I GGNCC 4 cut(s) 167, 1076, 1180, 1301
SchI GAGTC 4 cut(s) 968, 1275, 1325, 1373
ScrFI CCNGG 4 cut(s) 171, 677, 1179, 1407
SduI GDGCHC 3 cut(s) 795, 855, 1127
SfaNI GCATC 3 cut(s) 127, 383, 1446
SfcI CTRYAG 1 cut(s) 417
SmiMI CAYNNNNRTG 2 cut(s) 1088, 1232
SmlI CTYRAG 2 cut(s) 578, 1100
SmoI CTYRAG 2 cut(s) 578, 1100
SpeI ACTAGT 1 cut(s) 812
SsiI CCGC 3 cut(s) 83, 140, 1423
SspI AATATT 1 cut(s) 1207
SspMI CTAG 2 cut(s) 813, 1130
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 4 cut(s) 169, 675, 1177, 1405
StyI CCWWGG 3 cut(s) 651, 935, 1338
TaaI ACNGT 3 cut(s) 43, 96, 1231
TaiI ACGT 1 cut(s) 879
TaqI TCGA 5 cut(s) 12, 16, 1223, 1238, 1309
TaqII GACCGA 1 cut(s) 1230
TatI WGTACW 3 cut(s) 96, 909, 1347
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 2 cut(s) 1094, 1306
Tru1I TTAA 7 cut(s) 117, 252, 501, 540, 954, 1053, 1374
Tru9I TTAA 7 cut(s) 117, 252, 501, 540, 954, 1053, 1374
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 2 cut(s) 292, 419
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 5 cut(s) 444, 852, 941, 1040, 1143
TspGWI ACGGA 1 cut(s) 336
Van91I CCANNNNNTGG 1 cut(s) 1199
VneI GTGCAC 1 cut(s) 1123
XagI CCTNNNNNAGG 1 cut(s) 1275
XapI RAATTY 3 cut(s) 191, 495, 1133
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 4 cut(s) 207, 1039, 1199, 1439
XmiI GTMKAC 1 cut(s) 1430
XmnI GAANNNNTTC 3 cut(s) 435, 495, 565
XspI CTAG 2 cut(s) 813, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.