pycom16g03530

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000872
Physical Location & Seq
Forward (+)
2186855 .. 2193147
6293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03530.10

Sequence Viewer

Length: 2391 bp
ATGCCCGGCAAGGACGAGGGCTGCTCCTCCTCCGAGAAGGAGAAGAACCTCGAGCTCTTCCTGAAGGTCGGATTGGACGAGCGAACGGCCAAAAACACCATCGCCAACAACAAAGTCACCGCCAATCTCACCGCCGTCATTCACGAGGCGGCGGTTGAAGGATGCAGTCGAACAGTTGGTAATCTCCTCTACACGGTTGCCACGAAGCACCCAGCAAATGCCATTGTGCATCGCCCAAAATTGGCGCAATACATTGTATCGTCAAAGATTAAAACCCCTGCCCAGTTAGAAGCAGCTTTTTCATTTCTTGCAACGACAGGGCCAGAGAATTTTGATATCCATAAATTTGAAGAAGCTTGTGGTGTTGGTGTTGAAGTTTCCGAAGAAGAAATAAAACAAACTGTTAATGAGGTTTTTGAGGAGAATAAGACTGCAATTTTGGAGCAGCGGTATAGAACAAATGTGGGTGATTTGTTTGCACACGTTAGGAAGAGGCATCCGTGGGCTGATCCAAAAATTGTGAAGCAATTTATTGATGCAAAATTGCGTGAATTACTTGGTGAAAGGACAGCAGCAGATGATGAGAAGGTTCCAAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGTTGTTGCTGTTTCTACTCCAGAACAGCCATCTGAAGAAGTTCTTAATCCATACTCGATATTCCCTCAGCCATCGGATAATTATAAGGTTCATACTTCTGTTTTCTTTAGTGATGGTTCTATTTTGAGATGTTCCAATACAAGGGAATTGCTTGAGAAACACTTAAACAGGACTGGAGGGAAAGTTTTGACCCGCTTTCCTCCTGAACCGAATGGGTATCTACATATAGGTCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGGGATGGAGGCTGCTACCTACGGTTTGACGATACAAACCCTGAAGCTGAAAAGAAAGAATATATTGATCATATACAGGAAATCGTGAAATGGATGGGTTGGGAGCCCTACAAGATTACTTACACCAGTGATTACTTTCAAGATCTGTACGATTTAGCAGTGGAACTCATACGAAGAGGTCACGCTTATGTTGATCATCAGACAGCGGATGAGATAAAGGAGTACAGGGAGAAAAAGATGAACAGCCCTTGGCGGGACAGGCCAATTGCAGAGTCATTGAAACTTTTTGAGGATATGAGACGAGGCCTAATCGAAGAAGGCAAAGCAACCCTTAGAATGAAGCAAGACATGCAGAGTGATAATTATAACATGTATGACCTTATTGCATATCGTATCAAGTTTACACCCCATCCGCATGCTGGAGACAAGTGGTGTATCTATCCAAGTTATGATTATGCTCACTGCATTGTTGATTCTCTTGAGGATATCACACATTCGCTTTGCACACTTGAATTTGAGACTCGCCGTGCTTCGTACTACTGGTTATTAAATGCCCTCGGACTTTATCAGCCATATGTATGGGAATACTCACGACTGAATGTCAGTAACACTGTCATGTCAAAGCGTAAGTTAAATCGACTAGTGACACAAAACTGGGTTGATGGTTGGGATGACCCACGTCTTATGACACTGGCTGGTCTACGGCGTAGGGGTGTTACTTCAACAGCAATAAATGCTTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTAGTATGATACATTTAAGTCGCCTTGAGTATCACATAAGAGAGGAGCTAAATAAAACAGCTCCTCGCACGATAGCTGTACTACATCCCCTCAAGGTGGTTATTACAAACCTGGAAGCTGGCACTACAATGGATCTAGAAGCAAGGAAATGGCCTGATGCTCAAGCAGATGATGCATCTGCTTTCTACAAGGTTCCCTTTTCCAATACTGTTTATATTGACCGGTCTGATTTTCGGCTTAAAGATTCAAAGGATTACTTTGGGCTTGCTCCTGGTAAATCTGCCCTACTCAGATATGCATTCCCTATTAAGTGCACGGATGTCATATTAGCTGATGATAATGAAACTGTTCTTGAGATACAAGCCGAGTATGATCCGACAAAGAAGACAAAGCCAAAGGGGGTGCTTCACTGGGTTTCTGAACCTTCCCCAGGGGTTGATCCGCTCAAGGTGGAAATCAGATTGTTTGACCGACTGTTCCTTTCAGAGAATCCTGCTGAGCTCGAAGACTGGCTTGCTGATTTGAACCCACAATCCAAGGTGGTAATACCCAATGCATATGCTGTGCCCTCACTTGGAAGTGCTGTTGTTGGGGACACATTCCAGTTTGAAAGGCTCGGATATTTTGTAGTTGACAAGGATTCAACTCCTGAGAAGCTCGTGTTCAATCGTACTGTCACGCTGAGAGATAGCTATCGCAAGGGCGGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

797

Amino Acids

90.94

Weight (kDa)

6.28

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 717, 1272
AccBSI CCGCTC 1 cut(s) 2122
AccI GTMKAC 1 cut(s) 1606
AclWI GGATC 4 cut(s) 503, 1818, 2045, 2111
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 3 cut(s) 328, 344, 1418
AcuI CTGAAG 3 cut(s) 83, 687, 969
AfaI GTAC 5 cut(s) 1055, 1130, 1442, 1758, 2350
AfiI CCNNNNNNNGG 8 cut(s) 193, 241, 774, 1159, 1325, 1774, 2108, 2252
AflIII ACRYGT 2 cut(s) 481, 1275
AgeI ACCGGT 1 cut(s) 1899
AhdI GACNNNNNGTC 1 cut(s) 1505
AhlI ACTAGT 1 cut(s) 1546
AjiI CACGTC 1 cut(s) 1586
AjnI CCWGG 4 cut(s) 894, 1788, 1948, 2107
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AloI GAACNNNNNNTCC 2 cut(s) 2195, 2227
Alw21I GWGCWC 3 cut(s) 57, 1994, 2181
Alw26I GTCTC 3 cut(s) 1198, 1323, 1418
Alw44I GTGCAC 1 cut(s) 1990
AlwI GGATC 4 cut(s) 503, 1818, 2045, 2111
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 6 cut(s) 87, 320, 892, 1166, 1210, 1829
ApaLI GTGCAC 1 cut(s) 1990
ApeKI GCWGC 5 cut(s) 21, 293, 445, 572, 918
ApoI RAATTY 3 cut(s) 328, 344, 1418
ArsI GACNNNNNNTTYG 2 cut(s) 421, 453
AsiGI ACCGGT 1 cut(s) 1899
Asp700I GAANNNNTTC 2 cut(s) 672, 2025
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 5 cut(s) 109, 121, 479, 572, 1657
AvaI CYCGRG 1 cut(s) 50
BaeGI GKGCMC 2 cut(s) 1994, 2247
BanII GRGCYC 3 cut(s) 57, 1014, 2181
BauI CACGAG 2 cut(s) 143, 2336
BbsI GAAGAC 2 cut(s) 2069, 2190
Bbv12I GWGCWC 3 cut(s) 57, 1994, 2181
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 5 cut(s) 8, 305, 457, 584, 905
BccI CCATC 8 cut(s) 107, 670, 712, 740, 905, 994, 1323, 1562
BceAI ACGGC 4 cut(s) 102, 119, 1416, 1625
BciT130I CCWGG 4 cut(s) 896, 1790, 1950, 2109
BclI TGATCA 2 cut(s) 973, 1099
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 1198, 1323, 1418
BcuI ACTAGT 1 cut(s) 1546
BfaI CTAG 2 cut(s) 1547, 1814
BglII AGATCT 1 cut(s) 1048
BisI GCNGC 6 cut(s) 22, 150, 294, 446, 573, 919
BlpI GCTNAGC 1 cut(s) 2175
BlsI GCNGC 6 cut(s) 23, 151, 295, 447, 574, 920
Bme1390I CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
BmeRI GACNNNNNGTC 1 cut(s) 1505
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 1586
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 3 cut(s) 591, 1011, 1872
BmrFI CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
BmrI ACTGGG 3 cut(s) 277, 1570, 2098
BmsI GCATC 7 cut(s) 152, 238, 505, 526, 1825, 1840, 1862
BmuI ACTGGG 3 cut(s) 277, 1570, 2098
BoxI GACNNNNGTC 1 cut(s) 1584
BpiI GAAGAC 2 cut(s) 2069, 2190
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 3 cut(s) 636, 828, 1347
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 2175
BpuEI CTTGAG 7 cut(s) 806, 1406, 1724, 1754, 1824, 2051, 2108
BpuMI CCSGG 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 2370
BsaJI CCNNGG 7 cut(s) 500, 870, 1154, 1462, 2107, 2108, 2214
BsaWI WCCGGW 1 cut(s) 1899
BsaXI ACNNNNNCTCC 4 cut(s) 434, 464, 801, 831
Bsc4I CCNNNNNNNGG 8 cut(s) 193, 241, 774, 1159, 1325, 1774, 2108, 2252
Bse118I RCCGGY 1 cut(s) 1899
Bse1I ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
Bse3DI GCAATG 1 cut(s) 882
Bse8I GATNNNNATC 1 cut(s) 2370
BseBI CCWGG 4 cut(s) 896, 1790, 1950, 2109
BseDI CCNNGG 7 cut(s) 500, 870, 1154, 1462, 2107, 2108, 2214
BseGI GGATG 9 cut(s) 167, 496, 916, 1005, 1120, 1315, 1582, 1762, 2002
BseJI GATNNNNATC 1 cut(s) 2370
BseLI CCNNNNNNNGG 8 cut(s) 193, 241, 774, 1159, 1325, 1774, 2108, 2252
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 5 cut(s) 713, 1981, 2166, 2319, 2351
BseNI ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
BseRI GAGGAG 6 cut(s) 16, 19, 176, 434, 1731, 1736
BseSI GKGCMC 2 cut(s) 1994, 2247
BseXI GCAGC 5 cut(s) 8, 305, 457, 584, 905
BseYI CCCAGC 1 cut(s) 211
BshFI GGCC 6 cut(s) 89, 322, 894, 1168, 1212, 1831
BshTI ACCGGT 1 cut(s) 1899
BsiHKAI GWGCWC 3 cut(s) 57, 1994, 2181
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 6, 1900
BslFI GGGAC 2 cut(s) 1175, 2285
BslI CCNNNNNNNGG 8 cut(s) 193, 241, 774, 1159, 1325, 1774, 2108, 2252
BsmAI GTCTC 3 cut(s) 1198, 1323, 1418
BsmBI CGTCTC 1 cut(s) 1198
BsmFI GGGAC 2 cut(s) 1175, 2285
BsmI GAATGC 1 cut(s) 1976
BsnI GGCC 6 cut(s) 89, 322, 894, 1168, 1212, 1831
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 5 cut(s) 57, 1014, 1994, 2181, 2247
Bsp143I GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
Bsp1720I GCTNAGC 1 cut(s) 2175
BspANI GGCC 6 cut(s) 89, 322, 894, 1168, 1212, 1831
BspCNI CTCAG 5 cut(s) 712, 1980, 2167, 2320, 2352
BspLI GGNNCC 3 cut(s) 591, 1011, 1872
BspPI GGATC 4 cut(s) 503, 1818, 2045, 2111
BspQI GCTCTTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 2122
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 1899
BsrI ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
BssAI RCCGGY 1 cut(s) 1899
BssECI CCNNGG 7 cut(s) 500, 870, 1154, 1462, 2107, 2108, 2214
BssMI GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
BssSI CACGAG 2 cut(s) 143, 2336
BssT1I CCWWGG 3 cut(s) 870, 1154, 2214
Bst2BI CACGAG 2 cut(s) 143, 2336
Bst2UI CCWGG 4 cut(s) 896, 1790, 1950, 2109
Bst4CI ACNGT 9 cut(s) 175, 196, 403, 930, 1519, 1888, 2026, 2154, 2353
Bst6I CTCTTC 3 cut(s) 62, 485, 1075
BstAPI GCANNNNNTGC 3 cut(s) 1255, 1640, 1850
BstC8I GCNNGC 5 cut(s) 615, 1323, 1798, 1944, 2193
BstDEI CTNAG 6 cut(s) 699, 1238, 1967, 2175, 2328, 2360
BstDSI CCRYGG 1 cut(s) 500
BstENI CCTNNNNNAGG 1 cut(s) 2106
BstF5I GGATG 9 cut(s) 167, 496, 916, 1005, 1120, 1315, 1582, 1762, 2002
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
BstMAI GTCTC 3 cut(s) 1198, 1323, 1418
BstMBI GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
BstMWI GCNNNNNNNGC 4 cut(s) 1165, 1255, 1640, 1850
BstNI CCWGG 4 cut(s) 896, 1790, 1950, 2109
BstNSI RCATGY 3 cut(s) 1258, 1279, 1325
BstPAI GACNNNNGTC 1 cut(s) 1584
BstSCI CCNGG 5 cut(s) 4, 894, 1788, 1948, 2107
BstSLI GKGCMC 2 cut(s) 1994, 2247
BstV1I GCAGC 5 cut(s) 8, 305, 457, 584, 905
BstV2I GAAGAC 2 cut(s) 2069, 2190
BstX2I RGATCY 2 cut(s) 1048, 1810
BstXI CCANNNNNNTGG 1 cut(s) 1485
BstYI RGATCY 2 cut(s) 1048, 1810
BsuRI GGCC 6 cut(s) 89, 322, 894, 1168, 1212, 1831
BtgI CCRYGG 1 cut(s) 500
BtgZI GCGATG 2 cut(s) 85, 215
BtrI CACGTC 1 cut(s) 1586
BtsCI GGATG 9 cut(s) 167, 496, 916, 1005, 1120, 1315, 1582, 1762, 2002
BtsI GCAGTG 2 cut(s) 1071, 1366
BtsIMutI CAGTG 6 cut(s) 1039, 1071, 1366, 1515, 1595, 2086
Cac8I GCNNGC 5 cut(s) 615, 1323, 1798, 1944, 2193
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 1899
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 5 cut(s) 1054, 1129, 1441, 1757, 2349
CspAI ACCGGT 1 cut(s) 1899
CviAII CATG 5 cut(s) 866, 1255, 1276, 1322, 1522
CviQI GTAC 5 cut(s) 1054, 1129, 1441, 1757, 2349
DdeI CTNAG 6 cut(s) 699, 1238, 1967, 2175, 2328, 2360
DpnI GATC 7 cut(s) 510, 975, 1050, 1101, 1812, 2052, 2118
DpnII GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
DriI GACNNNNNGTC 1 cut(s) 1505
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 62, 485, 1075
Eam1105I GACNNNNNGTC 1 cut(s) 1505
EarI CTCTTC 3 cut(s) 62, 485, 1075
Ecl136II GAGCTC 2 cut(s) 55, 2179
Eco130I CCWWGG 3 cut(s) 870, 1154, 2214
Eco147I AGGCCT 1 cut(s) 1212
Eco24I GRGCYC 3 cut(s) 57, 1014, 2181
Eco32I GATATC 2 cut(s) 337, 1393
Eco53kI GAGCTC 2 cut(s) 55, 2179
Eco57I CTGAAG 3 cut(s) 83, 687, 969
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 2 cut(s) 55, 2179
EcoNI CCTNNNNNAGG 1 cut(s) 2106
EcoRII CCWGG 4 cut(s) 894, 1788, 1948, 2107
EcoRV GATATC 2 cut(s) 337, 1393
EcoT14I CCWWGG 3 cut(s) 870, 1154, 2214
EcoT22I ATGCAT 3 cut(s) 1855, 1978, 2236
EcoT38I GRGCYC 3 cut(s) 57, 1014, 2181
ErhI CCWWGG 3 cut(s) 870, 1154, 2214
Esp3I CGTCTC 1 cut(s) 1198
FaeI CATG 5 cut(s) 869, 1258, 1279, 1325, 1525
FaqI GGGAC 2 cut(s) 1175, 2285
FatI CATG 5 cut(s) 865, 1254, 1275, 1321, 1521
FauI CCCGC 3 cut(s) 833, 1152, 2375
FauNDI CATATG 2 cut(s) 1480, 2236
FbaI TGATCA 2 cut(s) 973, 1099
FblI GTMKAC 1 cut(s) 1606
Fnu4HI GCNGC 6 cut(s) 22, 150, 294, 446, 573, 919
FokI GGATG 9 cut(s) 174, 483, 923, 1012, 1127, 1302, 1589, 1749, 2009
FriOI GRGCYC 3 cut(s) 57, 1014, 2181
Fsp4HI GCNGC 6 cut(s) 22, 150, 294, 446, 573, 919
FspBI CTAG 2 cut(s) 1547, 1814
GlaI GCGC 1 cut(s) 246
GluI GCNGC 6 cut(s) 22, 150, 294, 446, 573, 919
GsaI CCCAGC 1 cut(s) 215
GsuI CTGGAG 3 cut(s) 636, 828, 1347
HaeIII GGCC 6 cut(s) 89, 322, 894, 1168, 1212, 1831
HapII CCGG 2 cut(s) 6, 1900
HhaI GCGC 1 cut(s) 247
Hin1II CATG 5 cut(s) 869, 1258, 1279, 1325, 1525
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 2311
HindII GTYRAC 1 cut(s) 2311
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 7 cut(s) 1178, 1379, 1426, 1662, 1922, 2167, 2318
HpaII CCGG 2 cut(s) 6, 1900
HphI GGTGA 5 cut(s) 109, 121, 479, 572, 1657
Hpy166II GTNNAC 4 cut(s) 1308, 1607, 1992, 2311
Hpy8I GTNNAC 4 cut(s) 1308, 1607, 1992, 2311
HpyAV CCTTC 6 cut(s) 31, 58, 152, 580, 1217, 2112
HpyCH4III ACNGT 9 cut(s) 175, 196, 403, 930, 1519, 1888, 2026, 2154, 2353
HpyCH4IV ACGT 2 cut(s) 483, 1585
HpyF10VI GCNNNNNNNGC 4 cut(s) 1165, 1255, 1640, 1850
HpyF3I CTNAG 6 cut(s) 699, 1238, 1967, 2175, 2328, 2360
HpySE526I ACGT 2 cut(s) 483, 1585
Hsp92II CATG 5 cut(s) 869, 1258, 1279, 1325, 1525
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 2 cut(s) 973, 1099
Kzo9I GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 6 cut(s) 29, 442, 1009, 1723, 1744, 1951
Lsp1109I GCAGC 5 cut(s) 8, 305, 457, 584, 905
LweI GCATC 7 cut(s) 152, 238, 505, 526, 1825, 1840, 1862
MaeI CTAG 2 cut(s) 1547, 1814
MaeII ACGT 2 cut(s) 483, 1585
MaeIII GTNAC 6 cut(s) 115, 1085, 1511, 1549, 1621, 2353
MalI GATC 7 cut(s) 510, 975, 1050, 1101, 1812, 2052, 2118
MbiI CCGCTC 1 cut(s) 2122
MboI GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
MfeI CAATTG 1 cut(s) 1170
MflI RGATCY 2 cut(s) 1048, 1810
MhlI GDGCHC 5 cut(s) 57, 1014, 1994, 2181, 2247
MlyI GAGTC 2 cut(s) 1187, 1420
MmeI TCCRAC 2 cut(s) 49, 2078
Mph1103I ATGCAT 3 cut(s) 1855, 1978, 2236
MroXI GAANNNNTTC 2 cut(s) 672, 2025
MseI TTAA 9 cut(s) 270, 405, 678, 797, 1454, 1538, 1694, 1917, 1986
MslI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
MspA1I CMGCKG 2 cut(s) 448, 1112
MspI CCGG 2 cut(s) 6, 1900
MspR9I CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
MunI CAATTG 1 cut(s) 1170
Mva1269I GAATGC 1 cut(s) 1976
MvaI CCWGG 4 cut(s) 896, 1790, 1950, 2109
MwoI GCNNNNNNNGC 4 cut(s) 1165, 1255, 1640, 1850
NciI CCSGG 1 cut(s) 6
NdeI CATATG 2 cut(s) 1480, 2236
NdeII GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
NlaIII CATG 5 cut(s) 869, 1258, 1279, 1325, 1525
NlaIV GGNNCC 3 cut(s) 591, 1011, 1872
NmeAIII GCCGAG 1 cut(s) 2068
NmuCI GTSAC 4 cut(s) 115, 1085, 1549, 2353
NsiI ATGCAT 3 cut(s) 1855, 1978, 2236
NspI RCATGY 3 cut(s) 1258, 1279, 1325
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 1 cut(s) 50
PasI CCCWGGG 1 cut(s) 2108
PceI AGGCCT 1 cut(s) 1212
PciI ACATGT 1 cut(s) 1275
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 200
PctI GAATGC 1 cut(s) 1976
PdmI GAANNNNTTC 2 cut(s) 672, 2025
PfeI GAWTC 5 cut(s) 1379, 1662, 1922, 2167, 2318
PinAI ACCGGT 1 cut(s) 1899
PkrI GCNGC 6 cut(s) 23, 151, 295, 447, 574, 920
PleI GAGTC 2 cut(s) 1186, 1420
PpsI GAGTC 2 cut(s) 1186, 1420
PscI ACATGT 1 cut(s) 1275
PshAI GACNNNNGTC 1 cut(s) 1584
PsiI TTATAA 2 cut(s) 717, 1272
Psp124BI GAGCTC 2 cut(s) 57, 2181
Psp6I CCWGG 4 cut(s) 894, 1788, 1948, 2107
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 4 cut(s) 894, 1788, 1948, 2107
PspN4I GGNNCC 3 cut(s) 591, 1011, 1872
PspPI GGNCC 1 cut(s) 320
PspXI VCTCGAGB 1 cut(s) 50
PsuI RGATCY 2 cut(s) 1048, 1810
RsaI GTAC 5 cut(s) 1055, 1130, 1442, 1758, 2350
RsaNI GTAC 5 cut(s) 1054, 1129, 1441, 1757, 2349
RseI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
SacI GAGCTC 2 cut(s) 57, 2181
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 9 cut(s) 270, 405, 678, 797, 1454, 1538, 1694, 1917, 1986
SatI GCNGC 6 cut(s) 22, 150, 294, 446, 573, 919
Sau3AI GATC 7 cut(s) 508, 973, 1048, 1099, 1810, 2050, 2116
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 2 cut(s) 1187, 1420
ScrFI CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
SduI GDGCHC 5 cut(s) 57, 1014, 1994, 2181, 2247
SfaNI GCATC 7 cut(s) 152, 238, 505, 526, 1825, 1840, 1862
Sfr274I CTCGAG 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
SmlI CTYRAG 8 cut(s) 50, 785, 1385, 1703, 1769, 1839, 2030, 2123
SmoI CTYRAG 8 cut(s) 50, 785, 1385, 1703, 1769, 1839, 2030, 2123
SpeI ACTAGT 1 cut(s) 1546
SphI GCATGC 1 cut(s) 1325
SseBI AGGCCT 1 cut(s) 1212
SspMI CTAG 2 cut(s) 1547, 1814
SstI GAGCTC 2 cut(s) 57, 2181
StuI AGGCCT 1 cut(s) 1212
StyD4I CCNGG 5 cut(s) 4, 894, 1788, 1948, 2107
StyI CCWWGG 3 cut(s) 870, 1154, 2214
TaaI ACNGT 9 cut(s) 175, 196, 403, 930, 1519, 1888, 2026, 2154, 2353
TaiI ACGT 2 cut(s) 486, 1588
TaqI TCGA 6 cut(s) 51, 169, 689, 1218, 1543, 2181
TaqII GACCGA 1 cut(s) 2163
TatI WGTACW 2 cut(s) 1128, 1756
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 5 cut(s) 1379, 1662, 1922, 2167, 2318
Tru1I TTAA 9 cut(s) 270, 405, 678, 797, 1454, 1538, 1694, 1917, 1986
Tru9I TTAA 9 cut(s) 270, 405, 678, 797, 1454, 1538, 1694, 1917, 1986
TscAI CASTG 6 cut(s) 1039, 1071, 1373, 1522, 1602, 2093
TseFI GTSAC 4 cut(s) 115, 1085, 1549, 2353
TseI GCWGC 5 cut(s) 21, 293, 445, 572, 918
Tsp45I GTSAC 4 cut(s) 115, 1085, 1549, 2353
TspDTI ATGAA 5 cut(s) 291, 713, 1160, 1259, 2034
TspGWI ACGGA 2 cut(s) 489, 2009
TspRI CASTG 6 cut(s) 1039, 1071, 1373, 1522, 1602, 2093
VneI GTGCAC 1 cut(s) 1990
XagI CCTNNNNNAGG 1 cut(s) 2106
XapI RAATTY 3 cut(s) 328, 344, 1418
XbaI TCTAGA 1 cut(s) 1813
XceI RCATGY 3 cut(s) 1258, 1279, 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 1606
XmnI GAANNNNTTC 2 cut(s) 672, 2025
XspI CTAG 2 cut(s) 1547, 1814
Zsp2I ATGCAT 3 cut(s) 1855, 1978, 2236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.