RchiOBHm_Chr4g0434881

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000872
Physical Location & Seq
Forward (+)
58527432 .. 58534980
7549 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40332

Sequence Viewer

Length: 2385 bp
ATGCCGGCCAAGGACGACGCCGCCGCCGAGAAGGAGAAGAACCTTGAGCTCTTCCTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACCATCGCCAACAACAAGGTCACGGCGAATCTAACGGCCGTCATTGACGAGGCTGGAGTTACTGATGGATGCAGTCGAACCGTTGGTAATCTTCTATACACGGTTGCTACAAAGTTTCCGGCAAATGCCCTTGTGCATCGCCCCGTGTTAACACAGTACATTGTGTCTTCAAAGATTAAAATCCCAGCTCAGTTAGATGCGGCTTTGTCATATTTTGTGAATACGGGGCCACAGAACTTCAAAACAACTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAACAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCCTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAAGTTGCGTGAATTGCTTGGTGAGAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTCTACTCCTGAACAACCAGCTGAAGAAATTCTAAATCCATATGCAATATTCCCTCAGCCAGACGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGTGTGACTGGGGGCAGAGTTTTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTATGTTGATCATCAGACAGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCACTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAAGCAACTCTAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTTATTGCGTATCGTATTAAGTTTACTCCTCATCCACATGCTGGAGACAAGTGGTGTATCTATCCGAGTTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATCACCCATTCGCTGTGCACTTTAGAATTTGAGACACGCCGTGCTTCATACTACTGGCTATTGCATGCTTTGGGCCTTTATCAACCATATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGTAAGTTAAATCGACTGGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTGGCTGGTTTACGACGTAGGGGTGTGACGTCGACTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTTGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCACGCACAATGGTTGTGCTTGATCCTGTAAAGGTTGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGATTGCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCTGATTTTCGCCTTAAAGACTCAAAAGATTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACGGGTCAAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGGTCGATCCACTCAAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTTCTGTTCCCCCACTTGGGAAAGGTGTTGGTGTTGGCGACAAGTTTCAGTTTGAAAGACTTGGGTATTTTGTGGTCGACAAAGATACGACTCCTGAGAAGCTTGTGTTTAATCGAACGGTCACGCTGCGAGATAGCTACAAGAGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

794

Amino Acids

90.79

Weight (kDa)

6.69

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 13 - 170 5.8e-55 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 173 - 248 2e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 272 - 576 4.6e-122 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 579 - 682 4.8e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 692 - 770 8.8e-15 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1625
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 2 cut(s) 1625, 2307
AccIII TCCGGA 2 cut(s) 943, 2047
AciI CCGC 5 cut(s) 21, 24, 76, 290, 1841
AclWI GGATC 4 cut(s) 1571, 1754, 2039, 2105
AcoI YGGCCR 3 cut(s) 6, 81, 126
AcsI RAATTY 3 cut(s) 341, 669, 1415
AcuI CTGAAG 3 cut(s) 77, 684, 711
AcyI GRCGYC 2 cut(s) 18, 1622
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 4 cut(s) 248, 1127, 1706, 1877
AfeI AGCGCT 2 cut(s) 446, 1886
AfiI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
AflIII ACRYGT 1 cut(s) 1272
AhdI GACNNNNNGTC 1 cut(s) 1502
AhlI ACTAGT 1 cut(s) 1028
AjnI CCWGG 4 cut(s) 497, 891, 1933, 2101
AjuI GAANNNNNNNTTGG 2 cut(s) 2215, 2247
AleI CACNNNNGTG 1 cut(s) 795
Alw21I GWGCWC 4 cut(s) 51, 1071, 1409, 1725
Alw26I GTCTC 4 cut(s) 484, 1320, 1415, 2009
Alw44I GTGCAC 1 cut(s) 1405
AlwI GGATC 4 cut(s) 1571, 1754, 2039, 2105
Aor13HI TCCGGA 2 cut(s) 943, 2047
Aor51HI AGCGCT 2 cut(s) 446, 1886
ApaLI GTGCAC 1 cut(s) 1405
ApeKI GCWGC 5 cut(s) 442, 569, 915, 2077, 2356
ApoI RAATTY 3 cut(s) 341, 669, 1415
ArsI GACNNNNNNTTYG 2 cut(s) 2112, 2144
AseI ATTAAT 1 cut(s) 1635
Asp700I GAANNNNTTC 2 cut(s) 585, 669
AspLEI GCGC 2 cut(s) 447, 1887
AspS9I GGNCC 3 cut(s) 317, 1462, 1827
AsuHPI GGTGA 5 cut(s) 476, 569, 1384, 1558, 1654
BaeGI GKGCMC 1 cut(s) 1409
BaeI ACNNNNGTAYC 2 cut(s) 1948, 1981
BanII GRGCYC 4 cut(s) 51, 1011, 1071, 1725
BbsI GAAGAC 2 cut(s) 249, 2063
Bbv12I GWGCWC 4 cut(s) 51, 1071, 1409, 1725
BbvCI CCTCAGC 2 cut(s) 696, 2004
BbvI GCAGC 5 cut(s) 454, 581, 902, 2064, 2343
BccI CCATC 5 cut(s) 101, 149, 737, 991, 1559
BceAI ACGGC 5 cut(s) 96, 113, 129, 141, 1413
BciT130I CCWGG 4 cut(s) 499, 893, 1935, 2103
BclI TGATCA 2 cut(s) 970, 1096
BcoDI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BcuI ACTAGT 1 cut(s) 1028
BfaI CTAG 1 cut(s) 1029
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 2 cut(s) 448, 1888
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 8 cut(s) 21, 24, 291, 443, 570, 916, 2078, 2357
BlpI GCTNAGC 1 cut(s) 2169
BlsI GCNGC 8 cut(s) 22, 25, 292, 444, 571, 917, 2079, 2358
Bme1390I CCNGG 4 cut(s) 499, 893, 1935, 2103
BmeRI GACNNNNNGTC 1 cut(s) 1502
BmgT120I GGNCC 3 cut(s) 317, 1462, 1827
BmiI GGNNCC 4 cut(s) 318, 588, 1008, 1857
BmrFI CCNGG 4 cut(s) 499, 893, 1935, 2103
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 5 cut(s) 149, 235, 277, 533, 1972
BmuI ACTGGG 1 cut(s) 813
BpiI GAAGAC 2 cut(s) 249, 2063
BplI GAGNNNNNCTC 2 cut(s) 1707, 1739
BpmI CTGGAG 2 cut(s) 165, 1344
Bpu10I CCTNAGC 2 cut(s) 696, 2004
Bpu1102I GCTNAGC 1 cut(s) 2169
BpuEI CTTGAG 5 cut(s) 65, 814, 1403, 1721, 2045
BsaBI GATNNNNATC 1 cut(s) 269
BsaHI GRCGYC 2 cut(s) 18, 1622
BsaJI CCNNGG 7 cut(s) 9, 497, 498, 867, 1151, 1866, 2102
BsaWI WCCGGW 2 cut(s) 943, 2047
Bsc4I CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 7 cut(s) 72, 808, 1448, 1548, 1599, 1790, 2186
Bse3DI GCAATG 2 cut(s) 879, 1363
Bse8I GATNNNNATC 1 cut(s) 269
BseAI TCCGGA 2 cut(s) 943, 2047
BseBI CCWGG 4 cut(s) 499, 893, 1935, 2103
BseDI CCNNGG 7 cut(s) 9, 497, 498, 867, 1151, 1866, 2102
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
BseMI GCAATG 2 cut(s) 879, 1363
BseNI ACTGG 7 cut(s) 72, 808, 1448, 1548, 1599, 1790, 2186
BseRI GAGGAG 1 cut(s) 1299
BseSI GKGCMC 1 cut(s) 1409
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 5 cut(s) 454, 581, 902, 2064, 2343
BseYI CCCAGC 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 4 cut(s) 51, 1071, 1409, 1725
BsiSI CCGG 4 cut(s) 5, 209, 944, 2048
BslFI GGGAC 1 cut(s) 1172
BslI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
BsmAI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BsmFI GGGAC 1 cut(s) 1172
Bsp1286I GDGCHC 5 cut(s) 51, 1011, 1071, 1409, 1725
Bsp13I TCCGGA 2 cut(s) 943, 2047
Bsp1407I TGTACA 1 cut(s) 1875
Bsp143I GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
Bsp1720I GCTNAGC 1 cut(s) 2169
BspACI CCGC 5 cut(s) 21, 24, 76, 290, 1841
BspEI TCCGGA 2 cut(s) 943, 2047
BspLI GGNNCC 4 cut(s) 318, 588, 1008, 1857
BspMAI CTGCAG 1 cut(s) 571
BspPI GGATC 4 cut(s) 1571, 1754, 2039, 2105
BspQI GCTCTTC 2 cut(s) 56, 1713
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 2 cut(s) 879, 1363
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 1875
BsrI ACTGG 7 cut(s) 72, 808, 1448, 1548, 1599, 1790, 2186
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 7 cut(s) 9, 497, 498, 867, 1151, 1866, 2102
BssMI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
BssNI GRCGYC 2 cut(s) 18, 1622
BssT1I CCWWGG 4 cut(s) 9, 867, 1151, 1866
Bst2UI CCWGG 4 cut(s) 499, 893, 1935, 2103
Bst4CI ACNGT 7 cut(s) 172, 193, 246, 1516, 1811, 2020, 2350
Bst6I CTCTTC 3 cut(s) 56, 482, 1713
BstACI GRCGYC 2 cut(s) 18, 1622
BstAUI TGTACA 1 cut(s) 1875
BstC8I GCNNGC 3 cut(s) 6, 612, 1455
BstENI CCTNNNNNAGG 1 cut(s) 1802
BstH2I RGCGCY 2 cut(s) 448, 1888
BstHHI GCGC 2 cut(s) 447, 1887
BstKTI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
BstMAI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BstMBI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 550
BstNI CCWGG 4 cut(s) 499, 893, 1935, 2103
BstNSI RCATGY 5 cut(s) 357, 1276, 1322, 1457, 1965
BstSCI CCNGG 4 cut(s) 497, 891, 1933, 2101
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 1409
BstV1I GCAGC 5 cut(s) 454, 581, 902, 2064, 2343
BstV2I GAAGAC 2 cut(s) 249, 2063
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 1 cut(s) 1045
BstZI CGGCCG 1 cut(s) 126
BtgZI GCGATG 2 cut(s) 79, 212
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 4 cut(s) 1068, 1178, 1512, 1592
Cac8I GCNNGC 3 cut(s) 6, 612, 1455
CfoI GCGC 2 cut(s) 447, 1887
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 317, 1462, 1827
CseI GACGC 1 cut(s) 26
Csp6I GTAC 4 cut(s) 247, 1126, 1705, 1876
CviAII CATG 6 cut(s) 354, 863, 1273, 1319, 1454, 1962
CviQI GTAC 4 cut(s) 247, 1126, 1705, 1876
DpnI GATC 9 cut(s) 972, 1019, 1047, 1098, 1578, 1761, 2046, 2112, 2193
DpnII GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
DraIII CACNNNGTG 1 cut(s) 1430
DriI GACNNNNNGTC 1 cut(s) 1502
EaeI YGGCCR 3 cut(s) 6, 81, 126
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 56, 482, 1713
Eam1105I GACNNNNNGTC 1 cut(s) 1502
EarI CTCTTC 3 cut(s) 56, 482, 1713
EciI GGCGGA 1 cut(s) 1856
Ecl136II GAGCTC 3 cut(s) 49, 1069, 1723
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 4 cut(s) 9, 867, 1151, 1866
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 4 cut(s) 51, 1011, 1071, 1725
Eco32I GATATC 1 cut(s) 848
Eco47III AGCGCT 2 cut(s) 446, 1886
Eco52I CGGCCG 1 cut(s) 126
Eco53kI GAGCTC 3 cut(s) 49, 1069, 1723
Eco57I CTGAAG 3 cut(s) 77, 684, 711
EcoICRI GAGCTC 3 cut(s) 49, 1069, 1723
EcoNI CCTNNNNNAGG 1 cut(s) 1802
EcoRII CCWGG 4 cut(s) 497, 891, 1933, 2101
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 4 cut(s) 9, 867, 1151, 1866
EcoT22I ATGCAT 1 cut(s) 2230
EcoT38I GRGCYC 4 cut(s) 51, 1011, 1071, 1725
ErhI CCWWGG 4 cut(s) 9, 867, 1151, 1866
FaeI CATG 6 cut(s) 357, 866, 1276, 1322, 1457, 1965
FaqI GGGAC 1 cut(s) 1172
FatI CATG 6 cut(s) 353, 862, 1272, 1318, 1453, 1961
FauNDI CATATG 2 cut(s) 682, 1477
FbaI TGATCA 2 cut(s) 970, 1096
FblI GTMKAC 2 cut(s) 1625, 2307
Fnu4HI GCNGC 8 cut(s) 21, 24, 291, 443, 570, 916, 2078, 2357
FriOI GRGCYC 4 cut(s) 51, 1011, 1071, 1725
Fsp4HI GCNGC 8 cut(s) 21, 24, 291, 443, 570, 916, 2078, 2357
FspBI CTAG 1 cut(s) 1029
GlaI GCGC 2 cut(s) 446, 1886
GluI GCNGC 8 cut(s) 21, 24, 291, 443, 570, 916, 2078, 2357
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 2 cut(s) 165, 1344
HaeII RGCGCY 2 cut(s) 448, 1888
HapII CCGG 4 cut(s) 5, 209, 944, 2048
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 447, 1887
Hin1I GRCGYC 2 cut(s) 18, 1622
Hin1II CATG 6 cut(s) 357, 866, 1276, 1322, 1457, 1965
Hin6I GCGC 2 cut(s) 445, 1885
HinP1I GCGC 2 cut(s) 445, 1885
HincII GTYRAC 3 cut(s) 240, 1626, 2308
HindII GTYRAC 3 cut(s) 240, 1626, 2308
HindIII AAGCTT 1 cut(s) 2330
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 4 cut(s) 5, 209, 944, 2048
HphI GGTGA 5 cut(s) 476, 569, 1384, 1558, 1654
Hpy166II GTNNAC 6 cut(s) 240, 1305, 1407, 1604, 1626, 2308
Hpy188III TCNNGA 9 cut(s) 55, 650, 833, 944, 980, 1382, 1494, 2048, 2324
Hpy8I GTNNAC 6 cut(s) 240, 1305, 1407, 1604, 1626, 2308
Hpy99I CGWCG 3 cut(s) 20, 1611, 1627
HpyAV CCTTC 6 cut(s) 25, 52, 952, 1071, 1214, 2106
HpyCH4III ACNGT 7 cut(s) 172, 193, 246, 1516, 1811, 2020, 2350
HpyCH4IV ACGT 3 cut(s) 1609, 1622, 1640
HpyF10VI GCNNNNNNNGC 1 cut(s) 550
HpySE526I ACGT 3 cut(s) 1609, 1622, 1640
Hsp92I GRCGYC 2 cut(s) 18, 1622
Hsp92II CATG 6 cut(s) 357, 866, 1276, 1322, 1457, 1965
HspAI GCGC 2 cut(s) 445, 1885
Kpn2I TCCGGA 2 cut(s) 943, 2047
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 970, 1096
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
LguI GCTCTTC 2 cut(s) 56, 1713
LmnI GCTCC 5 cut(s) 439, 1006, 1066, 1741, 1936
Lsp1109I GCAGC 5 cut(s) 454, 581, 902, 2064, 2343
LweI GCATC 5 cut(s) 149, 235, 277, 533, 1972
MaeI CTAG 1 cut(s) 1029
MaeII ACGT 3 cut(s) 1609, 1622, 1640
MalI GATC 9 cut(s) 972, 1019, 1047, 1098, 1578, 1761, 2046, 2112, 2193
MbiI CCGCTC 1 cut(s) 76
MboI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 5 cut(s) 51, 1011, 1071, 1409, 1725
MlyI GAGTC 4 cut(s) 1184, 1702, 1901, 2314
MmeI TCCRAC 1 cut(s) 43
Mph1103I ATGCAT 1 cut(s) 2230
MroI TCCGGA 2 cut(s) 943, 2047
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 585, 669
MslI CAYNNNNRTG 4 cut(s) 795, 1089, 1317, 1517
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 4 cut(s) 5, 209, 944, 2048
MspR9I CCNGG 4 cut(s) 499, 893, 1935, 2103
MunI CAATTG 1 cut(s) 991
MvaI CCWGG 4 cut(s) 499, 893, 1935, 2103
MwoI GCNNNNNNNGC 1 cut(s) 550
NaeI GCCGGC 1 cut(s) 6
NdeI CATATG 2 cut(s) 682, 1477
NdeII GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 357, 866, 1276, 1322, 1457, 1965
NlaIV GGNNCC 4 cut(s) 318, 588, 1008, 1857
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 8 cut(s) 109, 464, 799, 1082, 1176, 1546, 1618, 2350
NsiI ATGCAT 1 cut(s) 2230
NspI RCATGY 5 cut(s) 357, 1276, 1322, 1457, 1965
OliI CACNNNNGTG 1 cut(s) 795
PaeI GCATGC 1 cut(s) 1457
PasI CCCWGGG 1 cut(s) 498
PceI AGGCCT 1 cut(s) 1209
PciI ACATGT 1 cut(s) 1272
PciSI GCTCTTC 2 cut(s) 56, 1713
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 585, 669
PfeI GAWTC 6 cut(s) 118, 1376, 1659, 1684, 1832, 2161
PflMI CCANNNNNTGG 1 cut(s) 1322
PfoI TCCNGGA 1 cut(s) 1933
PkrI GCNGC 8 cut(s) 22, 25, 292, 444, 571, 917, 2079, 2358
PleI GAGTC 4 cut(s) 1183, 1701, 1901, 2314
PpsI GAGTC 4 cut(s) 1183, 1701, 1901, 2314
PscI ACATGT 1 cut(s) 1272
PshBI ATTAAT 1 cut(s) 1635
Psp124BI GAGCTC 3 cut(s) 51, 1071, 1725
Psp6I CCWGG 4 cut(s) 497, 891, 1933, 2101
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 4 cut(s) 497, 891, 1933, 2101
PspN4I GGNNCC 4 cut(s) 318, 588, 1008, 1857
PspPI GGNCC 3 cut(s) 317, 1462, 1827
PstI CTGCAG 1 cut(s) 571
PsuI RGATCY 1 cut(s) 1045
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 4 cut(s) 248, 1127, 1706, 1877
RsaNI GTAC 4 cut(s) 247, 1126, 1705, 1876
RseI CAYNNNNRTG 4 cut(s) 795, 1089, 1317, 1517
SacI GAGCTC 3 cut(s) 51, 1071, 1725
SalI GTCGAC 2 cut(s) 1624, 2306
SapI GCTCTTC 2 cut(s) 56, 1713
SatI GCNGC 8 cut(s) 21, 24, 291, 443, 570, 916, 2078, 2357
Sau3AI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2110, 2191
Sau96I GGNCC 3 cut(s) 317, 1462, 1827
SchI GAGTC 4 cut(s) 1184, 1702, 1901, 2314
ScrFI CCNGG 4 cut(s) 499, 893, 1935, 2103
SduI GDGCHC 5 cut(s) 51, 1011, 1071, 1409, 1725
SfaNI GCATC 5 cut(s) 149, 235, 277, 533, 1972
SfcI CTRYAG 1 cut(s) 567
SmiMI CAYNNNNRTG 4 cut(s) 795, 1089, 1317, 1517
SmlI CTYRAG 5 cut(s) 44, 793, 1382, 1700, 2024
SmoI CTYRAG 5 cut(s) 44, 793, 1382, 1700, 2024
SpeI ACTAGT 1 cut(s) 1028
SphI GCATGC 1 cut(s) 1457
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 5 cut(s) 21, 24, 76, 290, 1841
SspI AATATT 2 cut(s) 690, 1490
SspMI CTAG 1 cut(s) 1029
SstI GAGCTC 3 cut(s) 51, 1071, 1725
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 4 cut(s) 497, 891, 1933, 2101
StyI CCWWGG 4 cut(s) 9, 867, 1151, 1866
TaaI ACNGT 7 cut(s) 172, 193, 246, 1516, 1811, 2020, 2350
TaiI ACGT 3 cut(s) 1612, 1625, 1643
TaqI TCGA 8 cut(s) 166, 1523, 1540, 1625, 2109, 2207, 2307, 2344
TatI WGTACW 3 cut(s) 246, 1125, 1875
TauI GCSGC 3 cut(s) 23, 26, 293
TfiI GAWTC 6 cut(s) 118, 1376, 1659, 1684, 1832, 2161
TscAI CASTG 4 cut(s) 1068, 1185, 1519, 1599
TseFI GTSAC 8 cut(s) 109, 464, 799, 1082, 1176, 1546, 1618, 2350
TseI GCWGC 5 cut(s) 442, 569, 915, 2077, 2356
Tsp45I GTSAC 8 cut(s) 109, 464, 799, 1082, 1176, 1546, 1618, 2350
TspDTI ATGAA 7 cut(s) 594, 710, 1068, 1157, 1256, 1425, 1676
TspRI CASTG 4 cut(s) 1068, 1185, 1519, 1599
Van91I CCANNNNNTGG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 1405
VspI ATTAAT 1 cut(s) 1635
XagI CCTNNNNNAGG 1 cut(s) 1802
XapI RAATTY 3 cut(s) 341, 669, 1415
XceI RCATGY 5 cut(s) 357, 1276, 1322, 1457, 1965
XmiI GTMKAC 2 cut(s) 1625, 2307
XmnI GAANNNNTTC 2 cut(s) 585, 669
XspI CTAG 1 cut(s) 1029
ZraI GACGTC 1 cut(s) 1623
Zsp2I ATGCAT 1 cut(s) 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.