Rmu_sc0002328.1_g000004

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002328.1
OG0000872
Physical Location & Seq
Reverse (-)
28962 .. 36042
7081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002328.1_g000004.1.cds

Sequence Viewer

Length: 2619 bp
atgaacgcatacacaacaagatttgtcccaccttttgaagaatttgaagtcgactgctgtgatcttcctcagtctgcttcggtagtttttccctccgtcatagggccatcccttttcctagtcactgcagggaaaggcaggtttgatgcaggcttggctgaggatgatgtcgttacagaagatgatgatatagttgaagagggcgaggttctatttgtgcctgcccacaccaagattagcattactgctagatcaacagaactacaattgtataggcagctagcttcacagaccgacgcacttggttgtccttcgccgccgttcgagatgccggccaaggacgacgccgccgccgagaaggagaagaaccttgagctcttcctgaaggtcggactggatgagcggacggccaaaaacaccatcgccaacaacaaggtcacggcgaatctaacggccgtcattgacgaggctggagttactgatggatgcagtcgaaccgttggtaatcttctatacacggttgctacaaagtttccggcaaatgcccttgtgcatcgccccgtgttaacacagtacattgtgtcttcaaagattaaaatcccggctcagttagatgcggctttgtcatattttgtgaatacggggccggagaacttcaaaacaactgaatttgaagaagcatgtggtgttgtgggtgacttatttgcacatcttaggaagagacatccctgggctgacccaaaaattgtcaaggcatccattgatacaaagttgcgtgaattgcttggtgaaaggactgcagcagatgatgaaaaggttcctaaaaagaagaaagagaagcctgctaaagtagaggaaaaagctattgctgtttctactcctgaacaaccagctgaagaaattctaaatccatatgcaatattccctcagccagacgaaaactttaaggttcatacttcagttttctttagtgatggttctattctgagatgttgcaactcaaaggaactccttgaaaaacacttgagtgtgactgggggcagagttttaactcgctttcctcctgaaccgaatggatatctacatataggccatgccaaggcaatgtttattgactttggcctggcaaaagagagaggtggtggctgctatctgaggtttgatgacacaaatccggaagctgaaaagaaggaatatattgatcatattcaggaaattgtcaattggatgggttgggagccctacaagatcacttacactagtgattacttccaagatctgtatgaattagcagtggagctcatacgaaggggtcacgcttatgttgatcatcagacaggggatgagataaaggagtacagggagaaaaaaatgaacagcccttggagggacaggccaattaaagagtcgctgaaactttttgaggatatgaggcgaggcctgattgaagaagggaaagcaactctaagaatgaaacaagatatgcagagtgataattttaacatgtatgaccttattgcgtatcgtatcaagtttactcctcatccacatgctggagacaagtggtgtatctatccgagttacgattatgctcattgcattgtggattctcttgagaatatcacccattcgctgtgcactttagaatttgagacacgccgtgcttcatactactggctattgcatgctttggacctttatcaaccatatgtttgggaatattcaagactgaatgtcagtaacactgttatgtcgaagcgtaagttaaatcgactggtgactgaaaattgggttgatggttgggatgatccacgccttatgacactggctggtttacgacgtaggggtgtgacgtcgactgccattaatacgtttgttagaggaattggaatcaccagaagtgattgtagtatgattcatctgagtcgccttgagtaccacatcagagaagagctcaataaaacagctccacgcacaatggttgtgcttgatcctgtaaaggttgtcattaccaactggaaagatgacctcataaaggactgtgaagcaagaaagtggcccgattctgaggcggattgctacaaggttccgttttccaaggaggtgttgtacattgagcgctctgattttcgccttaaagactcaaaagattacttcggacttgctcctggaaaatctgccctgctcagatacgcatgtcctatcaagtgtgtggatgcaaaggatgttgttttagctgaggataatgagactgtccttgagatacgggtcaagtatgatccggagaagaagacaaagccaaagggggtgctgcattgggtcgctgaaccttctcctggggtcgatccactcaaagtggaagtccgattgtttgacagactattcaactcagagaatcctgctgagcttgatgagtggctttctgatctgaatggaaaatcgaaagtggtaataccaaatgcattttctgttcccccacttgggaaaggtgtcggtgttggcgacaagtttcagtttgaaagacttgggtattttgtggtcgacaaagatacgactcctgagaagcttgtgtttaatcgaacggtcacgctgcgagatagctacaagagtgggaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

872

Amino Acids

99.12

Weight (kDa)

5.77

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1856
Acc36I ACCTGC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 1553
AccBSI CCGCTC 1 cut(s) 403
AccI GTMKAC 3 cut(s) 51, 1856, 2541
AccIII TCCGGA 2 cut(s) 1174, 2281
AciI CCGC 6 cut(s) 317, 348, 351, 403, 617, 2072
AclWI GGATC 4 cut(s) 1802, 1985, 2273, 2339
AcoI YGGCCR 3 cut(s) 333, 408, 453
AcsI RAATTY 4 cut(s) 41, 668, 900, 1646
AcuI CTGAAG 3 cut(s) 404, 915, 942
AcyI GRCGYC 2 cut(s) 345, 1853
AdeI CACNNNGTG 1 cut(s) 1661
AfaI GTAC 4 cut(s) 575, 1358, 1937, 2111
AfeI AGCGCT 1 cut(s) 2120
AfiI CCNNNNNNNGG 8 cut(s) 102, 1099, 1387, 1553, 2035, 2336, 2480, 2481
AflIII ACRYGT 1 cut(s) 1503
AhdI GACNNNNNGTC 1 cut(s) 1733
AhlI ACTAGT 1 cut(s) 1259
AjnI CCWGG 4 cut(s) 728, 1122, 2167, 2335
AjuI GAANNNNNNNTTGG 2 cut(s) 2449, 2481
AleI CACNNNNGTG 1 cut(s) 1026
Alw21I GWGCWC 4 cut(s) 378, 1302, 1640, 1956
Alw26I GTCTC 4 cut(s) 715, 1551, 1646, 2243
Alw44I GTGCAC 1 cut(s) 1636
AlwI GGATC 4 cut(s) 1802, 1985, 2273, 2339
Aor13HI TCCGGA 2 cut(s) 1174, 2281
Aor51HI AGCGCT 1 cut(s) 2120
ApaLI GTGCAC 1 cut(s) 1636
ApeKI GCWGC 5 cut(s) 277, 800, 1146, 2311, 2590
ApoI RAATTY 4 cut(s) 41, 668, 900, 1646
ArsI GACNNNNNNTTYG 2 cut(s) 2346, 2378
AseI ATTAAT 1 cut(s) 1866
Asp700I GAANNNNTTC 2 cut(s) 816, 900
AspLEI GCGC 1 cut(s) 2121
AspS9I GGNCC 4 cut(s) 104, 644, 1693, 2058
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 5 cut(s) 707, 800, 1615, 1789, 1885
AsuNHI GCTAGC 1 cut(s) 280
AvaII GGWCC 1 cut(s) 1693
BaeGI GKGCMC 1 cut(s) 1640
BaeI ACNNNNGTAYC 2 cut(s) 2182, 2215
BanII GRGCYC 4 cut(s) 378, 1242, 1302, 1956
BbsI GAAGAC 2 cut(s) 576, 2297
Bbv12I GWGCWC 4 cut(s) 378, 1302, 1640, 1956
BbvCI CCTCAGC 3 cut(s) 159, 927, 2238
BbvI GCAGC 5 cut(s) 289, 812, 1133, 2298, 2577
BccI CCATC 6 cut(s) 115, 428, 476, 968, 1222, 1790
BceAI ACGGC 6 cut(s) 304, 423, 440, 456, 468, 1644
BciT130I CCWGG 4 cut(s) 730, 1124, 2169, 2337
BclI TGATCA 2 cut(s) 1201, 1327
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 4 cut(s) 715, 1551, 1646, 2243
BcuI ACTAGT 1 cut(s) 1259
BfaI CTAG 4 cut(s) 119, 249, 281, 1260
BfmI CTRYAG 2 cut(s) 126, 798
BfoI RGCGCY 1 cut(s) 2122
BfuAI ACCTGC 1 cut(s) 129
BglII AGATCT 1 cut(s) 1276
BisI GCNGC 9 cut(s) 278, 317, 348, 351, 618, 801, 1147, 2312, 2591
BlpI GCTNAGC 1 cut(s) 2403
BlsI GCNGC 9 cut(s) 279, 318, 349, 352, 619, 802, 1148, 2313, 2592
Bme1390I CCNGG 5 cut(s) 602, 730, 1124, 2169, 2337
Bme18I GGWCC 1 cut(s) 1693
BmeRI GACNNNNNGTC 1 cut(s) 1733
BmgT120I GGNCC 4 cut(s) 104, 644, 1693, 2058
BmiI GGNNCC 4 cut(s) 645, 819, 1239, 2088
BmrFI CCNGG 5 cut(s) 602, 730, 1124, 2169, 2337
BmrI ACTGGG 1 cut(s) 1044
BmsI GCATC 7 cut(s) 136, 318, 476, 562, 604, 764, 2206
BmtI GCTAGC 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 1044
BpiI GAAGAC 2 cut(s) 576, 2297
BplI GAGNNNNNCTC 2 cut(s) 1938, 1970
BpmI CTGGAG 2 cut(s) 492, 1575
Bpu10I CCTNAGC 3 cut(s) 159, 927, 2238
Bpu1102I GCTNAGC 1 cut(s) 2403
BpuEI CTTGAG 5 cut(s) 392, 1045, 1634, 1952, 2279
BpuMI CCSGG 1 cut(s) 602
BsaBI GATNNNNATC 1 cut(s) 596
BsaHI GRCGYC 2 cut(s) 345, 1853
BsaJI CCNNGG 7 cut(s) 336, 728, 729, 1098, 1382, 2097, 2336
BsaWI WCCGGW 2 cut(s) 1174, 2281
BsaXI ACNNNNNCTCC 2 cut(s) 2093, 2123
Bsc4I CCNNNNNNNGG 8 cut(s) 102, 1099, 1387, 1553, 2035, 2336, 2480, 2481
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 6 cut(s) 399, 1039, 1679, 1779, 1830, 2021
Bse3DI GCAATG 2 cut(s) 1110, 1594
Bse8I GATNNNNATC 1 cut(s) 596
BseAI TCCGGA 2 cut(s) 1174, 2281
BseBI CCWGG 4 cut(s) 730, 1124, 2169, 2337
BseDI CCNNGG 7 cut(s) 336, 728, 729, 1098, 1382, 2097, 2336
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 8 cut(s) 102, 1099, 1387, 1553, 2035, 2336, 2480, 2481
BseMI GCAATG 2 cut(s) 1110, 1594
BseNI ACTGG 6 cut(s) 399, 1039, 1679, 1779, 1830, 2021
BseRI GAGGAG 1 cut(s) 1530
BseSI GKGCMC 1 cut(s) 1640
BseX3I CGGCCG 1 cut(s) 453
BseXI GCAGC 5 cut(s) 289, 812, 1133, 2298, 2577
Bsh1285I CGRYCG 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 456
BsiHKAI GWGCWC 4 cut(s) 378, 1302, 1640, 1956
BsiSI CCGG 6 cut(s) 332, 536, 602, 647, 1175, 2282
BslFI GGGAC 2 cut(s) 11, 1403
BslI CCNNNNNNNGG 8 cut(s) 102, 1099, 1387, 1553, 2035, 2336, 2480, 2481
BsmAI GTCTC 4 cut(s) 715, 1551, 1646, 2243
BsmFI GGGAC 2 cut(s) 11, 1403
Bsp1286I GDGCHC 5 cut(s) 378, 1242, 1302, 1640, 1956
Bsp13I TCCGGA 2 cut(s) 1174, 2281
Bsp1407I TGTACA 1 cut(s) 2109
Bsp1720I GCTNAGC 1 cut(s) 2403
BspACI CCGC 6 cut(s) 317, 348, 351, 403, 617, 2072
BspEI TCCGGA 2 cut(s) 1174, 2281
BspLI GGNNCC 4 cut(s) 645, 819, 1239, 2088
BspMAI CTGCAG 2 cut(s) 130, 802
BspMI ACCTGC 1 cut(s) 129
BspOI GCTAGC 1 cut(s) 284
BspPI GGATC 4 cut(s) 1802, 1985, 2273, 2339
BspQI GCTCTTC 2 cut(s) 383, 1944
BsrBI CCGCTC 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 1110, 1594
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 2109
BsrI ACTGG 6 cut(s) 399, 1039, 1679, 1779, 1830, 2021
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 7 cut(s) 336, 728, 729, 1098, 1382, 2097, 2336
BssNI GRCGYC 2 cut(s) 345, 1853
BssT1I CCWWGG 4 cut(s) 336, 1098, 1382, 2097
Bst2UI CCWGG 4 cut(s) 730, 1124, 2169, 2337
Bst4CI ACNGT 7 cut(s) 499, 520, 573, 1747, 2042, 2254, 2584
Bst6I CTCTTC 4 cut(s) 192, 383, 713, 1944
BstACI GRCGYC 2 cut(s) 345, 1853
BstAUI TGTACA 1 cut(s) 2109
BstC8I GCNNGC 6 cut(s) 151, 222, 282, 333, 843, 1686
BstENI CCTNNNNNAGG 1 cut(s) 2033
BstH2I RGCGCY 1 cut(s) 2122
BstHHI GCGC 1 cut(s) 2121
BstMAI GTCTC 4 cut(s) 715, 1551, 1646, 2243
BstMCI CGRYCG 1 cut(s) 456
BstMWI GCNNNNNNNGC 2 cut(s) 155, 781
BstNI CCWGG 4 cut(s) 730, 1124, 2169, 2337
BstNSI RCATGY 5 cut(s) 684, 1507, 1553, 1688, 2199
BstSCI CCNGG 5 cut(s) 600, 728, 1122, 2167, 2335
BstSFI CTRYAG 2 cut(s) 126, 798
BstSLI GKGCMC 1 cut(s) 1640
BstV1I GCAGC 5 cut(s) 289, 812, 1133, 2298, 2577
BstV2I GAAGAC 2 cut(s) 576, 2297
BstX2I RGATCY 1 cut(s) 1276
BstXI CCANNNNNNTGG 1 cut(s) 1713
BstYI RGATCY 1 cut(s) 1276
BstZI CGGCCG 1 cut(s) 453
BtgZI GCGATG 2 cut(s) 406, 539
BtsI GCAGTG 2 cut(s) 123, 1299
BtsIMutI CAGTG 4 cut(s) 123, 1299, 1743, 1823
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 6 cut(s) 151, 222, 282, 333, 843, 1686
CfoI GCGC 1 cut(s) 2121
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 104, 644, 1693, 2058
CseI GACGC 2 cut(s) 305, 353
Csp6I GTAC 4 cut(s) 574, 1357, 1936, 2110
CviAII CATG 6 cut(s) 681, 1094, 1504, 1550, 1685, 2196
CviQI GTAC 4 cut(s) 574, 1357, 1936, 2110
DraIII CACNNNGTG 1 cut(s) 1661
DriI GACNNNNNGTC 1 cut(s) 1733
EaeI YGGCCR 3 cut(s) 333, 408, 453
EagI CGGCCG 1 cut(s) 453
Eam1104I CTCTTC 4 cut(s) 192, 383, 713, 1944
Eam1105I GACNNNNNGTC 1 cut(s) 1733
EarI CTCTTC 4 cut(s) 192, 383, 713, 1944
EciI GGCGGA 1 cut(s) 2087
Ecl136II GAGCTC 3 cut(s) 376, 1300, 1954
EclXI CGGCCG 1 cut(s) 453
Eco130I CCWWGG 4 cut(s) 336, 1098, 1382, 2097
Eco147I AGGCCT 1 cut(s) 1440
Eco24I GRGCYC 4 cut(s) 378, 1242, 1302, 1956
Eco32I GATATC 1 cut(s) 1079
Eco47I GGWCC 1 cut(s) 1693
Eco47III AGCGCT 1 cut(s) 2120
Eco52I CGGCCG 1 cut(s) 453
Eco53kI GAGCTC 3 cut(s) 376, 1300, 1954
Eco57I CTGAAG 3 cut(s) 404, 915, 942
EcoICRI GAGCTC 3 cut(s) 376, 1300, 1954
EcoNI CCTNNNNNAGG 1 cut(s) 2033
EcoRII CCWGG 4 cut(s) 728, 1122, 2167, 2335
EcoRV GATATC 1 cut(s) 1079
EcoT14I CCWWGG 4 cut(s) 336, 1098, 1382, 2097
EcoT22I ATGCAT 1 cut(s) 2464
EcoT38I GRGCYC 4 cut(s) 378, 1242, 1302, 1956
ErhI CCWWGG 4 cut(s) 336, 1098, 1382, 2097
FaeI CATG 6 cut(s) 684, 1097, 1507, 1553, 1688, 2199
FaqI GGGAC 2 cut(s) 11, 1403
FatI CATG 6 cut(s) 680, 1093, 1503, 1549, 1684, 2195
FauNDI CATATG 2 cut(s) 913, 1708
FbaI TGATCA 2 cut(s) 1201, 1327
FblI GTMKAC 3 cut(s) 51, 1856, 2541
Fnu4HI GCNGC 9 cut(s) 278, 317, 348, 351, 618, 801, 1147, 2312, 2591
FriOI GRGCYC 4 cut(s) 378, 1242, 1302, 1956
Fsp4HI GCNGC 9 cut(s) 278, 317, 348, 351, 618, 801, 1147, 2312, 2591
FspBI CTAG 4 cut(s) 119, 249, 281, 1260
GlaI GCGC 1 cut(s) 2120
GluI GCNGC 9 cut(s) 278, 317, 348, 351, 618, 801, 1147, 2312, 2591
GsuI CTGGAG 2 cut(s) 492, 1575
HaeII RGCGCY 1 cut(s) 2122
HapII CCGG 6 cut(s) 332, 536, 602, 647, 1175, 2282
HgaI GACGC 2 cut(s) 305, 353
HhaI GCGC 1 cut(s) 2121
Hin1I GRCGYC 2 cut(s) 345, 1853
Hin1II CATG 6 cut(s) 684, 1097, 1507, 1553, 1688, 2199
Hin6I GCGC 1 cut(s) 2119
HinP1I GCGC 1 cut(s) 2119
HincII GTYRAC 4 cut(s) 52, 567, 1857, 2542
HindII GTYRAC 4 cut(s) 52, 567, 1857, 2542
HindIII AAGCTT 1 cut(s) 2564
HpaI GTTAAC 1 cut(s) 567
HpaII CCGG 6 cut(s) 332, 536, 602, 647, 1175, 2282
HphI GGTGA 5 cut(s) 707, 800, 1615, 1789, 1885
Hpy166II GTNNAC 7 cut(s) 52, 567, 1536, 1638, 1835, 1857, 2542
Hpy8I GTNNAC 7 cut(s) 52, 567, 1536, 1638, 1835, 1857, 2542
Hpy99I CGWCG 4 cut(s) 299, 347, 1842, 1858
HpyAV CCTTC 7 cut(s) 321, 352, 379, 1183, 1302, 1445, 2340
HpyCH4III ACNGT 7 cut(s) 499, 520, 573, 1747, 2042, 2254, 2584
HpyCH4IV ACGT 3 cut(s) 1840, 1853, 1871
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 781
HpySE526I ACGT 3 cut(s) 1840, 1853, 1871
Hsp92I GRCGYC 2 cut(s) 345, 1853
Hsp92II CATG 6 cut(s) 684, 1097, 1507, 1553, 1688, 2199
HspAI GCGC 1 cut(s) 2119
Kpn2I TCCGGA 2 cut(s) 1174, 2281
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Ksp22I TGATCA 2 cut(s) 1201, 1327
KspAI GTTAAC 1 cut(s) 567
LguI GCTCTTC 2 cut(s) 383, 1944
LmnI GCTCC 4 cut(s) 1237, 1297, 1972, 2170
Lsp1109I GCAGC 5 cut(s) 289, 812, 1133, 2298, 2577
LweI GCATC 7 cut(s) 136, 318, 476, 562, 604, 764, 2206
MaeI CTAG 4 cut(s) 119, 249, 281, 1260
MaeII ACGT 3 cut(s) 1840, 1853, 1871
MbiI CCGCTC 1 cut(s) 403
MfeI CAATTG 2 cut(s) 266, 1222
MflI RGATCY 1 cut(s) 1276
MhlI GDGCHC 5 cut(s) 378, 1242, 1302, 1640, 1956
MlyI GAGTC 4 cut(s) 1415, 1933, 2135, 2548
MmeI TCCRAC 1 cut(s) 370
Mph1103I ATGCAT 1 cut(s) 2464
MroI TCCGGA 2 cut(s) 1174, 2281
MroNI GCCGGC 1 cut(s) 331
MroXI GAANNNNTTC 2 cut(s) 816, 900
MslI CAYNNNNRTG 4 cut(s) 1026, 1320, 1548, 1748
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 6 cut(s) 332, 536, 602, 647, 1175, 2282
MspR9I CCNGG 5 cut(s) 602, 730, 1124, 2169, 2337
MunI CAATTG 2 cut(s) 266, 1222
MvaI CCWGG 4 cut(s) 730, 1124, 2169, 2337
MwoI GCNNNNNNNGC 2 cut(s) 155, 781
NaeI GCCGGC 1 cut(s) 333
NciI CCSGG 1 cut(s) 602
NdeI CATATG 2 cut(s) 913, 1708
NgoMIV GCCGGC 1 cut(s) 331
NheI GCTAGC 1 cut(s) 280
NlaIII CATG 6 cut(s) 684, 1097, 1507, 1553, 1688, 2199
NlaIV GGNNCC 4 cut(s) 645, 819, 1239, 2088
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 8 cut(s) 121, 436, 695, 1030, 1313, 1777, 1849, 2584
NsiI ATGCAT 1 cut(s) 2464
NspI RCATGY 5 cut(s) 684, 1507, 1553, 1688, 2199
OliI CACNNNNGTG 1 cut(s) 1026
PaeI GCATGC 1 cut(s) 1688
PasI CCCWGGG 1 cut(s) 729
PceI AGGCCT 1 cut(s) 1440
PciI ACATGT 1 cut(s) 1503
PciSI GCTCTTC 2 cut(s) 383, 1944
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 2499
PdiI GCCGGC 1 cut(s) 333
PdmI GAANNNNTTC 2 cut(s) 816, 900
PfeI GAWTC 6 cut(s) 445, 1607, 1890, 1915, 2063, 2395
PflMI CCANNNNNTGG 1 cut(s) 1553
PfoI TCCNGGA 1 cut(s) 2167
PkrI GCNGC 9 cut(s) 279, 318, 349, 352, 619, 802, 1148, 2313, 2592
PleI GAGTC 4 cut(s) 1414, 1932, 2135, 2548
PpsI GAGTC 4 cut(s) 1414, 1932, 2135, 2548
PscI ACATGT 1 cut(s) 1503
PshBI ATTAAT 1 cut(s) 1866
Psp124BI GAGCTC 3 cut(s) 378, 1302, 1956
Psp6I CCWGG 4 cut(s) 728, 1122, 2167, 2335
PspGI CCWGG 4 cut(s) 728, 1122, 2167, 2335
PspN4I GGNNCC 4 cut(s) 645, 819, 1239, 2088
PspPI GGNCC 4 cut(s) 104, 644, 1693, 2058
PstI CTGCAG 2 cut(s) 130, 802
PsuI RGATCY 1 cut(s) 1276
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 4 cut(s) 575, 1358, 1937, 2111
RsaNI GTAC 4 cut(s) 574, 1357, 1936, 2110
RseI CAYNNNNRTG 4 cut(s) 1026, 1320, 1548, 1748
SacI GAGCTC 3 cut(s) 378, 1302, 1956
SalI GTCGAC 3 cut(s) 50, 1855, 2540
SapI GCTCTTC 2 cut(s) 383, 1944
SatI GCNGC 9 cut(s) 278, 317, 348, 351, 618, 801, 1147, 2312, 2591
Sau96I GGNCC 4 cut(s) 104, 644, 1693, 2058
SchI GAGTC 4 cut(s) 1415, 1933, 2135, 2548
ScrFI CCNGG 5 cut(s) 602, 730, 1124, 2169, 2337
SduI GDGCHC 5 cut(s) 378, 1242, 1302, 1640, 1956
SfaNI GCATC 7 cut(s) 136, 318, 476, 562, 604, 764, 2206
SfcI CTRYAG 2 cut(s) 126, 798
SinI GGWCC 1 cut(s) 1693
SmiMI CAYNNNNRTG 4 cut(s) 1026, 1320, 1548, 1748
SmlI CTYRAG 5 cut(s) 371, 1024, 1613, 1931, 2258
SmoI CTYRAG 5 cut(s) 371, 1024, 1613, 1931, 2258
SpeI ACTAGT 1 cut(s) 1259
SphI GCATGC 1 cut(s) 1688
SseBI AGGCCT 1 cut(s) 1440
SsiI CCGC 6 cut(s) 317, 348, 351, 403, 617, 2072
SspI AATATT 2 cut(s) 921, 1721
SspMI CTAG 4 cut(s) 119, 249, 281, 1260
SstI GAGCTC 3 cut(s) 378, 1302, 1956
StuI AGGCCT 1 cut(s) 1440
StyD4I CCNGG 5 cut(s) 600, 728, 1122, 2167, 2335
StyI CCWWGG 4 cut(s) 336, 1098, 1382, 2097
TaaI ACNGT 7 cut(s) 499, 520, 573, 1747, 2042, 2254, 2584
TaiI ACGT 3 cut(s) 1843, 1856, 1874
TaqII GACCGA 1 cut(s) 308
TatI WGTACW 3 cut(s) 573, 1356, 2109
TauI GCSGC 4 cut(s) 319, 350, 353, 620
TfiI GAWTC 6 cut(s) 445, 1607, 1890, 1915, 2063, 2395
TscAI CASTG 4 cut(s) 130, 1299, 1750, 1830
TseFI GTSAC 8 cut(s) 121, 436, 695, 1030, 1313, 1777, 1849, 2584
TseI GCWGC 5 cut(s) 277, 800, 1146, 2311, 2590
Tsp45I GTSAC 8 cut(s) 121, 436, 695, 1030, 1313, 1777, 1849, 2584
TspDTI ATGAA 8 cut(s) 17, 825, 941, 1299, 1388, 1487, 1656, 1907
TspGWI ACGGA 2 cut(s) 85, 2079
TspRI CASTG 4 cut(s) 130, 1299, 1750, 1830
Van91I CCANNNNNTGG 1 cut(s) 1553
VneI GTGCAC 1 cut(s) 1636
VpaK11BI GGWCC 1 cut(s) 1693
VspI ATTAAT 1 cut(s) 1866
XagI CCTNNNNNAGG 1 cut(s) 2033
XapI RAATTY 4 cut(s) 41, 668, 900, 1646
XceI RCATGY 5 cut(s) 684, 1507, 1553, 1688, 2199
XmiI GTMKAC 3 cut(s) 51, 1856, 2541
XmnI GAANNNNTTC 2 cut(s) 816, 900
XspI CTAG 4 cut(s) 119, 249, 281, 1260
ZraI GACGTC 1 cut(s) 1854
Zsp2I ATGCAT 1 cut(s) 2464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.