Rh6AG151800

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000872
Physical Location & Seq
Reverse (-)
23791568 .. 23798891
7324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG151800.1

Sequence Viewer

Length: 2394 bp
ATGCCTGCCAAGGACGACAGCGCCTCCGACAAGGAGAAGAGCCTTGAGCTCTTCTTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACAATCGCTAACCAGAAGGTCACCGCCAATCTAAGCGCGGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGGAGCGTCGGTAATCTTCTCTACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCCGTGTTAACACAGTACATTGTGTCTTCAAAGATTAAAATCCCAGCTCAGTTAGATGCGGCTTTGTCATTTTTTGCAAACACGGGGCCAGAAAACTTCAAAACAAGTGAATTTGAACAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAACAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCCAAAATTGTCAAGGCATCCATTGATACAAAATTGCATGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAACCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTCTACCCCTGAACAACCAGCTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGCGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACCTAAGTGTGACTGGCGGAAGAGTTTTAACTCGCTTTCCTCCTGAACCAAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAAGAATATATTGATCATATTCAAGAGATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTTCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCATGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCGCTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAGGCAACTCTAAGAATGAAACAAGACATGCAGAGCGATAATTTTAACATGTATGACCTTATTGCATATCGTATCAAGGTAAGTTTCTACCTCAAATGTTCTTTTTGGTTACCCAGGCTTATGGCTTCTCTCAAGCTGGTGATTTATTTATTTATTTTTCAGTTTACTCCTCATCCACATGCTGGAGACAACTGGTGTATCTATCCCAGTTACGATTATGCTCACTGCATTGTGGATTCTCTTGAGAATATCACGCATTCGCTGTGCAGTTTAGAATTTGAGACACGCCGTGCTTCATACTACTGGTTATTGCATTCTCTGGGCCTATATCAACCACATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGCAAGTTAAATCGACTAGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTACGACGCTGGCTGGTTTACGACGTAGGGGTGTGACGTCGAGTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTACTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCTGTAAAGGTCGTCATTACCAACTGGAAAGATGGCCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCATGTCCTATCAGGTGTGTGGATGCAAAGGATGTTGTTTTAGCCGAGGATAATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGATCGATCCACTCAAAGTGGAAGTTCGATTGTTTGACAGACTATTCAATTCAGAGAATCCTGCTGAGCTTGATAACTGGCTTTCTGATTTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCCCACTTGAGAAAGGTGTTGGTGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGTATGTTCCTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

797

Amino Acids

91.25

Weight (kDa)

6.55

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 13 - 170 7.1e-55 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 173 - 248 1.4e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 272 - 604 1.7e-113 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 607 - 710 2.6e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 720 - 795 9.3e-12 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1709
Acc36I ACCTGC 1 cut(s) 619
AccB7I CCANNNNNTGG 1 cut(s) 1406
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 128
AccIII TCCGGA 2 cut(s) 943, 2131
AciI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1925
AclWI GGATC 5 cut(s) 1655, 1838, 2123, 2189, 2198
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 3 cut(s) 341, 669, 1499
AcuI CTGAAG 2 cut(s) 684, 711
AcyI GRCGYC 1 cut(s) 1706
AdeI CACNNNGTG 1 cut(s) 1514
AfaI GTAC 5 cut(s) 248, 1127, 1762, 1790, 1961
AfeI AGCGCT 2 cut(s) 446, 1970
AfiI CCNNNNNNNGG 5 cut(s) 868, 1156, 1406, 1888, 2186
AflIII ACRYGT 2 cut(s) 1272, 1561
AhdI GACNNNNNGTC 1 cut(s) 1586
AhlI ACTAGT 2 cut(s) 1028, 1627
AjnI CCWGG 4 cut(s) 891, 1337, 2017, 2185
AleI CACNNNNGTG 1 cut(s) 795
Alw21I GWGCWC 3 cut(s) 51, 1071, 1809
Alw26I GTCTC 4 cut(s) 484, 1404, 1499, 2093
AlwI GGATC 5 cut(s) 1655, 1838, 2123, 2189, 2198
AlwNI CAGNNNCTG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 1784
Aor13HI TCCGGA 2 cut(s) 943, 2131
Aor51HI AGCGCT 2 cut(s) 446, 1970
AoxI GGCC 9 cut(s) 81, 317, 859, 889, 1163, 1207, 1546, 1879, 1910
ApeKI GCWGC 4 cut(s) 143, 569, 915, 2161
ApoI RAATTY 3 cut(s) 341, 669, 1499
AseI ATTAAT 1 cut(s) 1719
Asp700I GAANNNNTTC 2 cut(s) 585, 669
AspLEI GCGC 5 cut(s) 23, 128, 447, 1614, 1971
AspS9I GGNCC 3 cut(s) 317, 1546, 1911
AsuHPI GGTGA 6 cut(s) 103, 476, 569, 1375, 1738, 2363
AsuII TTCGAA 1 cut(s) 2367
AvaI CYCGRG 1 cut(s) 1784
BaeI ACNNNNGTAYC 4 cut(s) 1405, 1438, 2032, 2065
BanII GRGCYC 4 cut(s) 51, 1011, 1071, 1809
BarI GAAGNNNNNNTAC 2 cut(s) 1744, 1776
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 249, 2147
Bbv12I GWGCWC 3 cut(s) 51, 1071, 1809
BbvI GCAGC 4 cut(s) 130, 581, 902, 2148
BccI CCATC 5 cut(s) 149, 737, 991, 1643, 1871
BceAI ACGGC 2 cut(s) 96, 1497
BciT130I CCWGG 4 cut(s) 893, 1339, 2019, 2187
BclI TGATCA 2 cut(s) 970, 1096
BcoDI GTCTC 4 cut(s) 484, 1404, 1499, 2093
BcuI ACTAGT 2 cut(s) 1028, 1627
BfaI CTAG 2 cut(s) 1029, 1628
BfmI CTRYAG 2 cut(s) 144, 567
BfoI RGCGCY 3 cut(s) 24, 448, 1972
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 5 cut(s) 144, 291, 570, 916, 2162
BlpI GCTNAGC 1 cut(s) 2253
BlsI GCNGC 5 cut(s) 145, 292, 571, 917, 2163
Bme1390I CCNGG 4 cut(s) 893, 1339, 2019, 2187
BmeRI GACNNNNNGTC 1 cut(s) 1586
BmeT110I CYCGRG 1 cut(s) 1784
BmgT120I GGNCC 3 cut(s) 317, 1546, 1911
BmiI GGNNCC 4 cut(s) 318, 588, 1008, 1941
BmrFI CCNGG 4 cut(s) 893, 1339, 2019, 2187
BmrI ACTGGG 1 cut(s) 1425
BmsI GCATC 5 cut(s) 149, 235, 277, 533, 2056
BmuI ACTGGG 1 cut(s) 1425
BpiI GAAGAC 2 cut(s) 249, 2147
BplI GAGNNNNNCTC 2 cut(s) 1791, 1823
BpmI CTGGAG 1 cut(s) 1428
Bpu1102I GCTNAGC 1 cut(s) 2253
Bpu14I TTCGAA 1 cut(s) 2367
BpuEI CTTGAG 5 cut(s) 65, 1340, 1487, 2129, 2351
Bsa29I ATCGAT 1 cut(s) 2193
BsaBI GATNNNNATC 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 1706
BsaJI CCNNGG 8 cut(s) 9, 497, 867, 1151, 1337, 1950, 2088, 2186
BsaWI WCCGGW 2 cut(s) 943, 2131
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 160, 190
Bsc4I CCNNNNNNNGG 5 cut(s) 868, 1156, 1406, 1888, 2186
Bse1I ACTGG 7 cut(s) 72, 808, 1421, 1431, 1532, 1874, 2270
Bse3DI GCAATG 1 cut(s) 879
Bse8I GATNNNNATC 1 cut(s) 269
BseAI TCCGGA 2 cut(s) 943, 2131
BseBI CCWGG 4 cut(s) 893, 1339, 2019, 2187
BseCI ATCGAT 1 cut(s) 2193
BseDI CCNNGG 8 cut(s) 9, 497, 867, 1151, 1337, 1950, 2088, 2186
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 5 cut(s) 868, 1156, 1406, 1888, 2186
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 7 cut(s) 293, 746, 914, 1766, 1911, 2050, 2244
BseNI ACTGG 7 cut(s) 72, 808, 1421, 1431, 1532, 1874, 2270
BseRI GAGGAG 2 cut(s) 1383, 1812
BseXI GCAGC 4 cut(s) 130, 581, 902, 2148
BseYI CCCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 1510
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 9 cut(s) 83, 319, 861, 891, 1165, 1209, 1548, 1881, 1912
BshVI ATCGAT 1 cut(s) 2193
BsiHKAI GWGCWC 3 cut(s) 51, 1071, 1809
BsiHKCI CYCGRG 1 cut(s) 1784
BsiSI CCGG 2 cut(s) 944, 2132
BslFI GGGAC 1 cut(s) 1172
BslI CCNNNNNNNGG 5 cut(s) 868, 1156, 1406, 1888, 2186
BsmAI GTCTC 4 cut(s) 484, 1404, 1499, 2093
BsmFI GGGAC 1 cut(s) 1172
BsmI GAATGC 2 cut(s) 1480, 1537
BsnI GGCC 9 cut(s) 83, 319, 861, 891, 1165, 1209, 1548, 1881, 1912
BsoBI CYCGRG 1 cut(s) 1784
Bsp119I TTCGAA 1 cut(s) 2367
Bsp1286I GDGCHC 4 cut(s) 51, 1011, 1071, 1809
Bsp13I TCCGGA 2 cut(s) 943, 2131
Bsp1407I TGTACA 1 cut(s) 1959
Bsp143I GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
Bsp1720I GCTNAGC 1 cut(s) 2253
BspACI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1925
BspANI GGCC 9 cut(s) 83, 319, 861, 891, 1165, 1209, 1548, 1881, 1912
BspCNI CTCAG 7 cut(s) 292, 747, 915, 1767, 1912, 2049, 2245
BspDI ATCGAT 1 cut(s) 2193
BspEI TCCGGA 2 cut(s) 943, 2131
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 4 cut(s) 318, 588, 1008, 1941
BspMAI CTGCAG 2 cut(s) 148, 571
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 5 cut(s) 1655, 1838, 2123, 2189, 2198
BspQI GCTCTTC 3 cut(s) 32, 56, 1797
BspT104I TTCGAA 1 cut(s) 2367
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 879
BsrGI TGTACA 1 cut(s) 1959
BsrI ACTGG 7 cut(s) 72, 808, 1421, 1431, 1532, 1874, 2270
BssECI CCNNGG 8 cut(s) 9, 497, 867, 1151, 1337, 1950, 2088, 2186
BssMI GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
BssNI GRCGYC 1 cut(s) 1706
BssSI CACGAG 1 cut(s) 137
BssT1I CCWWGG 4 cut(s) 9, 867, 1151, 1950
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 4 cut(s) 893, 1339, 2019, 2187
Bst4CI ACNGT 5 cut(s) 193, 246, 1600, 1895, 2104
Bst6I CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1797
BstACI GRCGYC 1 cut(s) 1706
BstAUI TGTACA 1 cut(s) 1959
BstBI TTCGAA 1 cut(s) 2367
BstC8I GCNNGC 2 cut(s) 6, 1680
BstDSI CCRYGG 1 cut(s) 497
BstEII GGTNACC 2 cut(s) 109, 1332
BstENI CCTNNNNNAGG 1 cut(s) 1886
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 3 cut(s) 24, 448, 1972
BstHHI GCGC 5 cut(s) 23, 128, 447, 1614, 1971
BstKTI GATC 9 cut(s) 973, 1020, 1048, 1099, 1663, 1846, 2131, 2193, 2197
BstMAI GTCTC 4 cut(s) 484, 1404, 1499, 2093
BstMBI GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 4 cut(s) 893, 1339, 2019, 2187
BstNSI RCATGY 6 cut(s) 357, 1255, 1276, 1406, 1565, 2049
BstPI GGTNACC 2 cut(s) 109, 1332
BstSCI CCNGG 4 cut(s) 891, 1337, 2017, 2185
BstSFI CTRYAG 2 cut(s) 144, 567
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 130, 581, 902, 2148
BstV2I GAAGAC 2 cut(s) 249, 2147
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 2 cut(s) 1345, 1566
BstYI RGATCY 1 cut(s) 1045
Bsu15I ATCGAT 1 cut(s) 2193
BsuRI GGCC 9 cut(s) 83, 319, 861, 891, 1165, 1209, 1548, 1881, 1912
BsuTUI ATCGAT 1 cut(s) 2193
BtgI CCRYGG 1 cut(s) 497
BtgZI GCGATG 2 cut(s) 212, 756
BtsI GCAGTG 2 cut(s) 1068, 1447
BtsIMutI CAGTG 3 cut(s) 1068, 1447, 1596
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 6, 1680
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 5 cut(s) 23, 128, 447, 1614, 1971
Cfr13I GGNCC 3 cut(s) 317, 1546, 1911
ClaI ATCGAT 1 cut(s) 2193
CseI GACGC 2 cut(s) 160, 1684
Csp6I GTAC 5 cut(s) 247, 1126, 1761, 1789, 1960
CviAII CATG 9 cut(s) 354, 545, 863, 1085, 1252, 1273, 1403, 1562, 2046
CviQI GTAC 5 cut(s) 247, 1126, 1761, 1789, 1960
DpnI GATC 9 cut(s) 972, 1019, 1047, 1098, 1662, 1845, 2130, 2192, 2196
DpnII GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
DraIII CACNNNGTG 1 cut(s) 1514
DriI GACNNNNNGTC 1 cut(s) 1586
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1797
Eam1105I GACNNNNNGTC 1 cut(s) 1586
EarI CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1797
EciI GGCGGA 2 cut(s) 822, 1940
Ecl136II GAGCTC 3 cut(s) 49, 1069, 1807
Eco130I CCWWGG 4 cut(s) 9, 867, 1151, 1950
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 4 cut(s) 51, 1011, 1071, 1809
Eco32I GATATC 1 cut(s) 848
Eco47III AGCGCT 2 cut(s) 446, 1970
Eco53kI GAGCTC 3 cut(s) 49, 1069, 1807
Eco57I CTGAAG 2 cut(s) 684, 711
Eco88I CYCGRG 1 cut(s) 1784
Eco91I GGTNACC 2 cut(s) 109, 1332
EcoICRI GAGCTC 3 cut(s) 49, 1069, 1807
EcoNI CCTNNNNNAGG 1 cut(s) 1886
EcoO65I GGTNACC 2 cut(s) 109, 1332
EcoRII CCWGG 4 cut(s) 891, 1337, 2017, 2185
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 4 cut(s) 9, 867, 1151, 1950
EcoT22I ATGCAT 1 cut(s) 2314
EcoT38I GRGCYC 4 cut(s) 51, 1011, 1071, 1809
ErhI CCWWGG 4 cut(s) 9, 867, 1151, 1950
FaeI CATG 9 cut(s) 357, 548, 866, 1088, 1255, 1276, 1406, 1565, 2049
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 1172
FatI CATG 9 cut(s) 353, 544, 862, 1084, 1251, 1272, 1402, 1561, 2045
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 2 cut(s) 970, 1096
Fnu4HI GCNGC 5 cut(s) 144, 291, 570, 916, 2162
FriOI GRGCYC 4 cut(s) 51, 1011, 1071, 1809
Fsp4HI GCNGC 5 cut(s) 144, 291, 570, 916, 2162
FspBI CTAG 2 cut(s) 1029, 1628
GlaI GCGC 5 cut(s) 22, 127, 446, 1613, 1970
GluI GCNGC 5 cut(s) 144, 291, 570, 916, 2162
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 1428
HaeII RGCGCY 3 cut(s) 24, 448, 1972
HaeIII GGCC 9 cut(s) 83, 319, 861, 891, 1165, 1209, 1548, 1881, 1912
HapII CCGG 2 cut(s) 944, 2132
HgaI GACGC 2 cut(s) 160, 1684
HhaI GCGC 5 cut(s) 23, 128, 447, 1614, 1971
Hin1I GRCGYC 1 cut(s) 1706
Hin1II CATG 9 cut(s) 357, 548, 866, 1088, 1255, 1276, 1406, 1565, 2049
Hin6I GCGC 5 cut(s) 21, 126, 445, 1612, 1969
HinP1I GCGC 5 cut(s) 21, 126, 445, 1612, 1969
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 9 cut(s) 1175, 1460, 1743, 1768, 1777, 1916, 1928, 1991, 2245
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 2 cut(s) 944, 2132
HphI GGTGA 6 cut(s) 103, 476, 569, 1375, 1738, 2363
Hpy166II GTNNAC 3 cut(s) 240, 1389, 1688
Hpy188III TCNNGA 9 cut(s) 55, 137, 833, 944, 980, 1043, 1466, 1578, 2132
Hpy8I GTNNAC 3 cut(s) 240, 1389, 1688
Hpy99I CGWCG 4 cut(s) 176, 1678, 1695, 1711
HpyAV CCTTC 6 cut(s) 52, 100, 1071, 1214, 1219, 2190
HpyCH4III ACNGT 5 cut(s) 193, 246, 1600, 1895, 2104
HpyCH4IV ACGT 4 cut(s) 1092, 1693, 1706, 1724
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpySE526I ACGT 4 cut(s) 1092, 1693, 1706, 1724
Hsp92I GRCGYC 1 cut(s) 1706
Hsp92II CATG 9 cut(s) 357, 548, 866, 1088, 1255, 1276, 1406, 1565, 2049
HspAI GCGC 5 cut(s) 21, 126, 445, 1612, 1969
Kpn2I TCCGGA 2 cut(s) 943, 2131
Ksp22I TGATCA 2 cut(s) 970, 1096
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
LguI GCTCTTC 3 cut(s) 32, 56, 1797
LmnI GCTCC 6 cut(s) 168, 1006, 1066, 1825, 1976, 2020
Lsp1109I GCAGC 4 cut(s) 130, 581, 902, 2148
LweI GCATC 5 cut(s) 149, 235, 277, 533, 2056
MaeI CTAG 2 cut(s) 1029, 1628
MaeII ACGT 4 cut(s) 1092, 1693, 1706, 1724
MalI GATC 9 cut(s) 972, 1019, 1047, 1098, 1662, 1845, 2130, 2192, 2196
MbiI CCGCTC 1 cut(s) 76
MboI GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 4 cut(s) 51, 1011, 1071, 1809
MlyI GAGTC 4 cut(s) 1184, 1786, 1937, 1985
MmeI TCCRAC 3 cut(s) 43, 51, 146
Mph1103I ATGCAT 1 cut(s) 2314
MroI TCCGGA 2 cut(s) 943, 2131
MroXI GAANNNNTTC 2 cut(s) 585, 669
MslI CAYNNNNRTG 3 cut(s) 795, 1401, 1601
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 2 cut(s) 944, 2132
MspR9I CCNGG 4 cut(s) 893, 1339, 2019, 2187
MunI CAATTG 1 cut(s) 991
Mva1269I GAATGC 2 cut(s) 1480, 1537
MvaI CCWGG 4 cut(s) 893, 1339, 2019, 2187
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 168
NdeI CATATG 1 cut(s) 682
NdeII GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
NlaIII CATG 9 cut(s) 357, 548, 866, 1088, 1255, 1276, 1406, 1565, 2049
NlaIV GGNNCC 4 cut(s) 318, 588, 1008, 1941
NmeAIII GCCGAG 1 cut(s) 2113
NmuCI GTSAC 6 cut(s) 109, 464, 799, 1630, 1702, 2351
NsiI ATGCAT 1 cut(s) 2314
NspI RCATGY 6 cut(s) 357, 1255, 1276, 1406, 1565, 2049
NspV TTCGAA 1 cut(s) 2367
OliI CACNNNNGTG 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 1784
PceI AGGCCT 1 cut(s) 1209
PciI ACATGT 2 cut(s) 1272, 1561
PciSI GCTCTTC 3 cut(s) 32, 56, 1797
PctI GAATGC 2 cut(s) 1480, 1537
PdmI GAANNNNTTC 2 cut(s) 585, 669
PfeI GAWTC 5 cut(s) 1460, 1743, 1768, 1916, 2245
PflMI CCANNNNNTGG 1 cut(s) 1406
PfoI TCCNGGA 1 cut(s) 2017
PkrI GCNGC 5 cut(s) 145, 292, 571, 917, 2163
PleI GAGTC 4 cut(s) 1183, 1785, 1936, 1985
PpsI GAGTC 4 cut(s) 1183, 1785, 1936, 1985
PscI ACATGT 2 cut(s) 1272, 1561
PshBI ATTAAT 1 cut(s) 1719
Psp124BI GAGCTC 3 cut(s) 51, 1071, 1809
Psp6I CCWGG 4 cut(s) 891, 1337, 2017, 2185
PspEI GGTNACC 2 cut(s) 109, 1332
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 4 cut(s) 891, 1337, 2017, 2185
PspN4I GGNNCC 4 cut(s) 318, 588, 1008, 1941
PspPI GGNCC 3 cut(s) 317, 1546, 1911
PspXI VCTCGAGB 1 cut(s) 1784
PstI CTGCAG 2 cut(s) 148, 571
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 1045
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 5 cut(s) 248, 1127, 1762, 1790, 1961
RsaNI GTAC 5 cut(s) 247, 1126, 1761, 1789, 1960
RseI CAYNNNNRTG 3 cut(s) 795, 1401, 1601
SacI GAGCTC 3 cut(s) 51, 1071, 1809
SapI GCTCTTC 3 cut(s) 32, 56, 1797
SatI GCNGC 5 cut(s) 144, 291, 570, 916, 2162
Sau3AI GATC 9 cut(s) 970, 1017, 1045, 1096, 1660, 1843, 2128, 2190, 2194
Sau96I GGNCC 3 cut(s) 317, 1546, 1911
SchI GAGTC 4 cut(s) 1184, 1786, 1937, 1985
ScrFI CCNGG 4 cut(s) 893, 1339, 2019, 2187
SduI GDGCHC 4 cut(s) 51, 1011, 1071, 1809
SfaNI GCATC 5 cut(s) 149, 235, 277, 533, 2056
SfcI CTRYAG 2 cut(s) 144, 567
Sfr274I CTCGAG 1 cut(s) 1784
SfuI TTCGAA 1 cut(s) 2367
SlaI CTCGAG 1 cut(s) 1784
SmiMI CAYNNNNRTG 3 cut(s) 795, 1401, 1601
SmlI CTYRAG 6 cut(s) 44, 1355, 1466, 1784, 2108, 2330
SmoI CTYRAG 6 cut(s) 44, 1355, 1466, 1784, 2108, 2330
SpeI ACTAGT 2 cut(s) 1028, 1627
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1925
SspI AATATT 1 cut(s) 1574
SspMI CTAG 2 cut(s) 1029, 1628
SstI GAGCTC 3 cut(s) 51, 1071, 1809
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 4 cut(s) 891, 1337, 2017, 2185
StyI CCWWGG 4 cut(s) 9, 867, 1151, 1950
TaaI ACNGT 5 cut(s) 193, 246, 1600, 1895, 2104
TaiI ACGT 4 cut(s) 1095, 1696, 1709, 1727
TaqI TCGA 9 cut(s) 1607, 1624, 1709, 1785, 2121, 2193, 2215, 2291, 2367
TatI WGTACW 4 cut(s) 246, 1125, 1760, 1959
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 5 cut(s) 1460, 1743, 1768, 1916, 2245
TscAI CASTG 3 cut(s) 1068, 1454, 1603
TseFI GTSAC 6 cut(s) 109, 464, 799, 1630, 1702, 2351
TseI GCWGC 4 cut(s) 143, 569, 915, 2161
Tsp45I GTSAC 6 cut(s) 109, 464, 799, 1630, 1702, 2351
TspDTI ATGAA 8 cut(s) 561, 594, 710, 1068, 1157, 1256, 1509, 1760
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 3 cut(s) 1068, 1454, 1603
Van91I CCANNNNNTGG 1 cut(s) 1406
VspI ATTAAT 1 cut(s) 1719
XagI CCTNNNNNAGG 1 cut(s) 1886
XapI RAATTY 3 cut(s) 341, 669, 1499
XceI RCATGY 6 cut(s) 357, 1255, 1276, 1406, 1565, 2049
XhoI CTCGAG 1 cut(s) 1784
XmnI GAANNNNTTC 2 cut(s) 585, 669
XspI CTAG 2 cut(s) 1029, 1628
ZraI GACGTC 1 cut(s) 1707
Zsp2I ATGCAT 1 cut(s) 2314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.