MD13G1039800.v1.1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000872
Physical Location & Seq
Forward (+)
2711530 .. 2718199
6670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1039800.v1.1.491

Sequence Viewer

Length: 2391 bp
ATGCCCGGCAAGGACGACGCCGGCTCCTCCGAGAAGGAGAAGAACCTCGAGCTCTTCCTCAAAGTCGGATTGGACGAGCGCACGGCCAAGAACACCATCGCCAACAAAAAAGTCACCGCCAACCTCACCGCCGTCATTCATGAGGCGGCGGTTACTGATGGATGCAATCGAACAGTTGGTAATCTCCTCTACACGGTTGCGACAAAGCACCCAGCAAATGCCCTTGTGCATCGCCCAACATTGGCGCAATACATTGTATCGTCGAAGATTAAAACCCCTGCCCAGTTAGAAGCGGCTTTTTCATTTTTTGCAACGACGGGGCCAGAGAATTTTGAAGTAAATAAATTTGAAGAAGCTTGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATAAAACAAACTGTTAATGAGGTTTTTGAGGAGAATAAGGCTGCAATCTTGGAGCAGCGGTATAGAACAAATGTGGGGGATTTGTTTGCACATGTTAGGAGGAGGCATCCGTGGGCTGATCCAAAAATTGTAAAGCAATTTATTGATGCAAAATTGCGTGAATTACTTGGTGAAAGGACAGCAGCAGATGATGAAAAGGTTCCAAAAAAGAAAAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGTTGATGCTGTTTCTACTCCAGAACAGCCATCTGAAGAAGTTCTTAATCCATACTCAATATTCCCTCAGCCATTGGATAATTATAAGGTTCATACTTCTGTTTTCTTTAGTGACGGTTCTATTTTGAGATGTTGCAATACAAGGGAATTGCTTGAGAAACACTTAAACAGGACTGGTGGGAAAGTTTTGACCCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGTCATGCCAAGGCAATGTTTATCGACTTTGGCCTGGCAAAAGAGAGGGATGGAGGCTGCTACCTACGGTTTGACGATACAAACCCAGAAGCTGAAAAGAAAGAATATATTGATCATATTCAGGAAATTGTGAAGTGGATGGGTTGGGAGCCCTACAAGATCACTTACACCAGTGACTACTTTCAAGATTTATATGATTTTGCAGTGGAACTCATACGAAGGGGTCATGCATATGTAGATCATCAGACACCTGACGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGCGGGACAGGCCAATTGCAGAGTCATTGAAACTTTTTGAGGATATGAGACGAGGCCTGATTGAAGAAGGCAAAGCAACCCTTAGAATGAAGCAAGACATGCAGAGTGATAACTTTAACATGTATGACCTTATTGCATATCGTATCAAGTTTACACCCCATCCTCATGCTGGAGACAAGTGGTGTATCTATCCAAGTTATGATTATTCTCACTGCATTGTTGATTCTCTTGAGGATATCACACATTCGCTTTGCACACTTGAATTTGAGACTCGCCGTGCTTCGTACTACTGGTTATTAAATGCCCTCGGAATCTATCAACCATATGTATGGGAATACTCACGGCTGAATGTCAGTAACACTGTCATGTCAAAGCGTAAGTTAAATCGACTAGTGACTCAGAACTGGGTTGACGGTTGGGATGACCCACGTCTTATGACACTGGCTGGTTTACGACGCAGGGGTGTTACTCCAACTGCAATAAATGCTTTTGTTAGAGGAATTGGAATCACTAGAAGTGATTGTAGTATGATACACTTAAGTCGCCTTGAGTATCACATAAGAGAGGAGCTAAATAAAACAGCTCCTCGAACGATAGCTGTGCTACATCCCCTCAAGGTGGTTATTACAAACCTGGAAGCTGGCACTACAATGGATCTAGAAGCAAGGAAATGGCCTGATGCTCAGACAGATGATGCATCTGCTTTCTACAAGGTTCCCTTTTCCAATACCGTTTATATTGACCGGTCTGATTTTCGGCTTAAAGATTCAAAGGATTACTTTGGGCTTGCTCCTGGTAAATCTGTCCTACTCAGATATGCGTTTCCTATCAAGTGCACAGATGTCATATTAGCTGATGATGAGGAAACTGTTCTTGAGATACGGGCTGAGTATGATCCTACAAAGAAGACAAAGCCAAAGGGGGTGCTTCACTGGGTTTCTGAACCTTCTCCAGGGGTTGATCCACTCAAGGTGGAAGTCAGATTGTTTGACCGACTGTTCCTTTCAGAGAATCCTGCTGAGCTCGAAGACTGGCTTGCTGATTTGAATCCAGAGTCCAAGGTGGTAATACCCAATGCATATGCTGTGCCCTCACTTGGAAGTGCTGTTGTTGGGGACGCATTCCAGTTTGAAAGGCTCGGATATTTTGTAGTCGATAAGGATTCAACTCCTGAGAAGCTCGTCTTCAACCGTACCGTCACGCTGAGAGATAGCTATCACAAGGGCGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

797

Amino Acids

91.09

Weight (kDa)

6.15

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 14 - 171 3.3e-56 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 174 - 249 2.8e-06 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 272 - 577 1.6e-122 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 580 - 684 2.7e-19 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 694 - 771 8.7e-19 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AciI CCGC 9 cut(s) 117, 129, 146, 149, 293, 448, 826, 1159, 2382
AclWI GGATC 4 cut(s) 503, 1818, 2045, 2111
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 328, 344, 1418
AcuI CTGAAG 1 cut(s) 687
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 1130, 1442, 2350
AfiI CCNNNNNNNGG 7 cut(s) 193, 241, 1159, 1325, 1774, 2108, 2252
AflII CTTAAG 1 cut(s) 1693
AflIII ACRYGT 2 cut(s) 481, 1275
AgeI ACCGGT 1 cut(s) 1899
AhlI ACTAGT 1 cut(s) 1546
AjiI CACGTC 1 cut(s) 1586
AjnI CCWGG 4 cut(s) 894, 1788, 1948, 2107
Alw21I GWGCWC 3 cut(s) 54, 1994, 2181
Alw26I GTCTC 3 cut(s) 1198, 1323, 1418
Alw44I GTGCAC 1 cut(s) 1990
AlwI GGATC 4 cut(s) 503, 1818, 2045, 2111
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 6 cut(s) 84, 320, 892, 1166, 1210, 1829
ApaLI GTGCAC 1 cut(s) 1990
ApeKI GCWGC 4 cut(s) 431, 445, 572, 918
ApoI RAATTY 3 cut(s) 328, 344, 1418
AsiGI ACCGGT 1 cut(s) 1899
Asp700I GAANNNNTTC 3 cut(s) 588, 672, 2025
AspLEI GCGC 2 cut(s) 81, 247
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 3 cut(s) 106, 118, 572
AvaI CYCGRG 1 cut(s) 47
BaeGI GKGCMC 2 cut(s) 1994, 2247
BanII GRGCYC 3 cut(s) 54, 1014, 2181
BbsI GAAGAC 3 cut(s) 2069, 2190, 2332
Bbv12I GWGCWC 3 cut(s) 54, 1994, 2181
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 4 cut(s) 418, 457, 584, 905
BccI CCATC 6 cut(s) 104, 152, 670, 905, 994, 1323
BceAI ACGGC 4 cut(s) 99, 116, 1416, 1514
BcgI CGANNNNNNTGC 2 cut(s) 1738, 1772
BciT130I CCWGG 4 cut(s) 896, 1790, 1950, 2109
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 1198, 1323, 1418
BcuI ACTAGT 1 cut(s) 1546
BfaI CTAG 3 cut(s) 1547, 1668, 1814
BfrI CTTAAG 1 cut(s) 1693
BisI GCNGC 6 cut(s) 147, 294, 432, 446, 573, 919
BlpI GCTNAGC 1 cut(s) 2175
BlsI GCNGC 6 cut(s) 148, 295, 433, 447, 574, 920
Bme1390I CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 1586
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 5 cut(s) 25, 321, 591, 1011, 1872
BmrFI CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
BmrI ACTGGG 3 cut(s) 277, 1570, 2098
BmsI GCATC 8 cut(s) 152, 238, 505, 526, 628, 1825, 1840, 1862
BmuI ACTGGG 3 cut(s) 277, 1570, 2098
BoxI GACNNNNGTC 1 cut(s) 1584
BpiI GAAGAC 3 cut(s) 2069, 2190, 2332
BpmI CTGGAG 3 cut(s) 636, 1347, 2091
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 2175
BpuEI CTTGAG 6 cut(s) 806, 1406, 1724, 1754, 2051, 2108
BpuMI CCSGG 1 cut(s) 6
BsaBI GATNNNNATC 2 cut(s) 2202, 2370
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 6 cut(s) 500, 870, 1154, 1462, 2108, 2214
BsaWI WCCGGW 1 cut(s) 1899
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 434, 464
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 241, 1159, 1325, 1774, 2108, 2252
Bse118I RCCGGY 2 cut(s) 20, 1899
Bse1I ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
Bse3DI GCAATG 1 cut(s) 882
Bse8I GATNNNNATC 2 cut(s) 2202, 2370
BseBI CCWGG 4 cut(s) 896, 1790, 1950, 2109
BseDI CCNNGG 6 cut(s) 500, 870, 1154, 1462, 2108, 2214
BseGI GGATG 7 cut(s) 167, 496, 916, 1005, 1315, 1582, 1762
BseJI GATNNNNATC 2 cut(s) 2202, 2370
BseLI CCNNNNNNNGG 7 cut(s) 193, 241, 1159, 1325, 1774, 2108, 2252
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 8 cut(s) 713, 1568, 1853, 1981, 2034, 2166, 2319, 2351
BseNI ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
BseRI GAGGAG 6 cut(s) 16, 176, 434, 505, 1731, 1736
BseSI GKGCMC 2 cut(s) 1994, 2247
BseXI GCAGC 4 cut(s) 418, 457, 584, 905
BseYI CCCAGC 1 cut(s) 211
BshFI GGCC 6 cut(s) 86, 322, 894, 1168, 1212, 1831
BshTI ACCGGT 1 cut(s) 1899
BsiHKAI GWGCWC 3 cut(s) 54, 1994, 2181
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 3 cut(s) 6, 21, 1900
BslFI GGGAC 2 cut(s) 1175, 2285
BslI CCNNNNNNNGG 7 cut(s) 193, 241, 1159, 1325, 1774, 2108, 2252
BsmAI GTCTC 3 cut(s) 1198, 1323, 1418
BsmBI CGTCTC 1 cut(s) 1198
BsmFI GGGAC 2 cut(s) 1175, 2285
BsmI GAATGC 1 cut(s) 2276
BsnI GGCC 6 cut(s) 86, 322, 894, 1168, 1212, 1831
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 5 cut(s) 54, 1014, 1994, 2181, 2247
Bsp143I GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
Bsp1720I GCTNAGC 1 cut(s) 2175
BspACI CCGC 9 cut(s) 117, 129, 146, 149, 293, 448, 826, 1159, 2382
BspANI GGCC 6 cut(s) 86, 322, 894, 1168, 1212, 1831
BspCNI CTCAG 8 cut(s) 712, 1567, 1852, 1980, 2035, 2167, 2320, 2352
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 5 cut(s) 25, 321, 591, 1011, 1872
BspPI GGATC 4 cut(s) 503, 1818, 2045, 2111
BspQI GCTCTTC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 1693
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 2 cut(s) 20, 1899
BsrI ACTGG 9 cut(s) 283, 811, 1032, 1451, 1565, 1602, 2093, 2192, 2281
BssAI RCCGGY 2 cut(s) 20, 1899
BssECI CCNNGG 6 cut(s) 500, 870, 1154, 1462, 2108, 2214
BssMI GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 870, 1154, 2214
Bst2UI CCWGG 4 cut(s) 896, 1790, 1950, 2109
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 1693
BstAPI GCANNNNNTGC 2 cut(s) 1255, 1640
BstC8I GCNNGC 5 cut(s) 22, 615, 1798, 1944, 2193
BstDEI CTNAG 9 cut(s) 699, 1238, 1554, 1839, 1967, 2043, 2175, 2328, 2360
BstDSI CCRYGG 1 cut(s) 500
BstENI CCTNNNNNAGG 1 cut(s) 2106
BstF5I GGATG 7 cut(s) 167, 496, 916, 1005, 1315, 1582, 1762
BstHHI GCGC 2 cut(s) 81, 247
BstKTI GATC 7 cut(s) 511, 976, 1023, 1102, 1813, 2053, 2119
BstMAI GTCTC 3 cut(s) 1198, 1323, 1418
BstMBI GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
BstMWI GCNNNNNNNGC 3 cut(s) 1165, 1255, 1640
BstNI CCWGG 4 cut(s) 896, 1790, 1950, 2109
BstNSI RCATGY 3 cut(s) 485, 1258, 1279
BstPAI GACNNNNGTC 1 cut(s) 1584
BstSCI CCNGG 5 cut(s) 4, 894, 1788, 1948, 2107
BstSLI GKGCMC 2 cut(s) 1994, 2247
BstV1I GCAGC 4 cut(s) 418, 457, 584, 905
BstV2I GAAGAC 3 cut(s) 2069, 2190, 2332
BstX2I RGATCY 1 cut(s) 1810
BstXI CCANNNNNNTGG 1 cut(s) 1485
BstYI RGATCY 1 cut(s) 1810
BsuRI GGCC 6 cut(s) 86, 322, 894, 1168, 1212, 1831
BtgI CCRYGG 1 cut(s) 500
BtgZI GCGATG 2 cut(s) 82, 215
BtrI CACGTC 1 cut(s) 1586
BtsCI GGATG 7 cut(s) 167, 496, 916, 1005, 1315, 1582, 1762
BtsI GCAGTG 2 cut(s) 1071, 1366
BtsIMutI CAGTG 6 cut(s) 1039, 1071, 1366, 1515, 1595, 2086
Cac8I GCNNGC 5 cut(s) 22, 615, 1798, 1944, 2193
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 1 cut(s) 139
CfoI GCGC 2 cut(s) 81, 247
Cfr10I RCCGGY 2 cut(s) 20, 1899
Cfr13I GGNCC 1 cut(s) 320
CseI GACGC 3 cut(s) 26, 1620, 2282
Csp6I GTAC 3 cut(s) 1129, 1441, 2349
CspAI ACCGGT 1 cut(s) 1899
CviAII CATG 8 cut(s) 140, 482, 866, 1088, 1255, 1276, 1322, 1522
CviQI GTAC 3 cut(s) 1129, 1441, 2349
DdeI CTNAG 9 cut(s) 699, 1238, 1554, 1839, 1967, 2043, 2175, 2328, 2360
DpnI GATC 7 cut(s) 510, 975, 1022, 1101, 1812, 2052, 2118
DpnII GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 2 cut(s) 52, 2179
Eco130I CCWWGG 3 cut(s) 870, 1154, 2214
Eco147I AGGCCT 1 cut(s) 1212
Eco24I GRGCYC 3 cut(s) 54, 1014, 2181
Eco32I GATATC 2 cut(s) 851, 1393
Eco53kI GAGCTC 2 cut(s) 52, 2179
Eco57I CTGAAG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 2 cut(s) 52, 2179
EcoNI CCTNNNNNAGG 1 cut(s) 2106
EcoRII CCWGG 4 cut(s) 894, 1788, 1948, 2107
EcoRV GATATC 2 cut(s) 851, 1393
EcoT14I CCWWGG 3 cut(s) 870, 1154, 2214
EcoT22I ATGCAT 3 cut(s) 1093, 1855, 2236
EcoT38I GRGCYC 3 cut(s) 54, 1014, 2181
ErhI CCWWGG 3 cut(s) 870, 1154, 2214
Esp3I CGTCTC 1 cut(s) 1198
FaeI CATG 8 cut(s) 143, 485, 869, 1091, 1258, 1279, 1325, 1525
FaqI GGGAC 2 cut(s) 1175, 2285
FatI CATG 8 cut(s) 139, 481, 865, 1087, 1254, 1275, 1321, 1521
FauI CCCGC 3 cut(s) 833, 1152, 2375
FauNDI CATATG 3 cut(s) 1093, 1480, 2236
FbaI TGATCA 1 cut(s) 973
Fnu4HI GCNGC 6 cut(s) 147, 294, 432, 446, 573, 919
FokI GGATG 7 cut(s) 174, 483, 923, 1012, 1302, 1589, 1749
FriOI GRGCYC 3 cut(s) 54, 1014, 2181
Fsp4HI GCNGC 6 cut(s) 147, 294, 432, 446, 573, 919
FspBI CTAG 3 cut(s) 1547, 1668, 1814
GlaI GCGC 2 cut(s) 80, 246
GluI GCNGC 6 cut(s) 147, 294, 432, 446, 573, 919
GsaI CCCAGC 1 cut(s) 215
GsuI CTGGAG 3 cut(s) 636, 1347, 2091
HaeIII GGCC 6 cut(s) 86, 322, 894, 1168, 1212, 1831
HapII CCGG 3 cut(s) 6, 21, 1900
HgaI GACGC 3 cut(s) 26, 1620, 2282
HhaI GCGC 2 cut(s) 81, 247
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 8 cut(s) 143, 485, 869, 1091, 1258, 1279, 1325, 1525
Hin6I GCGC 2 cut(s) 79, 245
HinP1I GCGC 2 cut(s) 79, 245
HincII GTYRAC 1 cut(s) 1567
HindII GTYRAC 1 cut(s) 1567
HindIII AAGCTT 1 cut(s) 354
HpaII CCGG 3 cut(s) 6, 21, 1900
HphI GGTGA 3 cut(s) 106, 118, 572
Hpy166II GTNNAC 4 cut(s) 1308, 1567, 1607, 1992
Hpy8I GTNNAC 4 cut(s) 1308, 1567, 1607, 1992
Hpy99I CGWCG 4 cut(s) 20, 265, 319, 1614
HpyAV CCTTC 4 cut(s) 28, 1074, 1217, 2112
HpyCH4IV ACGT 1 cut(s) 1585
HpyF10VI GCNNNNNNNGC 3 cut(s) 1165, 1255, 1640
HpyF3I CTNAG 9 cut(s) 699, 1238, 1554, 1839, 1967, 2043, 2175, 2328, 2360
HpySE526I ACGT 1 cut(s) 1585
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 8 cut(s) 143, 485, 869, 1091, 1258, 1279, 1325, 1525
HspAI GCGC 2 cut(s) 79, 245
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 6 cut(s) 29, 442, 1009, 1723, 1744, 1951
Lsp1109I GCAGC 4 cut(s) 418, 457, 584, 905
LweI GCATC 8 cut(s) 152, 238, 505, 526, 628, 1825, 1840, 1862
MaeI CTAG 3 cut(s) 1547, 1668, 1814
MaeII ACGT 1 cut(s) 1585
MaeIII GTNAC 8 cut(s) 112, 151, 743, 1034, 1511, 1549, 1621, 2353
MalI GATC 7 cut(s) 510, 975, 1022, 1101, 1812, 2052, 2118
MboI GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
MfeI CAATTG 1 cut(s) 1170
MflI RGATCY 1 cut(s) 1810
MhlI GDGCHC 5 cut(s) 54, 1014, 1994, 2181, 2247
MlyI GAGTC 4 cut(s) 1187, 1420, 1546, 2219
MmeI TCCRAC 2 cut(s) 46, 1652
Mph1103I ATGCAT 3 cut(s) 1093, 1855, 2236
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 3 cut(s) 588, 672, 2025
MseI TTAA 9 cut(s) 270, 405, 678, 797, 1272, 1454, 1538, 1694, 1917
MslI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
MspA1I CMGCKG 1 cut(s) 448
MspCI CTTAAG 1 cut(s) 1693
MspI CCGG 3 cut(s) 6, 21, 1900
MspR9I CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
MunI CAATTG 1 cut(s) 1170
Mva1269I GAATGC 1 cut(s) 2276
MvaI CCWGG 4 cut(s) 896, 1790, 1950, 2109
MwoI GCNNNNNNNGC 3 cut(s) 1165, 1255, 1640
NaeI GCCGGC 1 cut(s) 22
NciI CCSGG 1 cut(s) 6
NdeI CATATG 3 cut(s) 1093, 1480, 2236
NdeII GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 8 cut(s) 143, 485, 869, 1091, 1258, 1279, 1325, 1525
NlaIV GGNNCC 5 cut(s) 25, 321, 591, 1011, 1872
NmuCI GTSAC 5 cut(s) 112, 743, 1034, 1549, 2353
NsiI ATGCAT 3 cut(s) 1093, 1855, 2236
NspI RCATGY 3 cut(s) 485, 1258, 1279
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 139
PceI AGGCCT 1 cut(s) 1212
PciI ACATGT 2 cut(s) 481, 1275
PciSI GCTCTTC 1 cut(s) 59
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 2276
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 3 cut(s) 588, 672, 2025
PfeI GAWTC 7 cut(s) 1379, 1467, 1662, 1922, 2167, 2203, 2318
PinAI ACCGGT 1 cut(s) 1899
PkrI GCNGC 6 cut(s) 148, 295, 433, 447, 574, 920
PleI GAGTC 4 cut(s) 1186, 1420, 1546, 2218
PpsI GAGTC 4 cut(s) 1186, 1420, 1546, 2218
PscI ACATGT 2 cut(s) 481, 1275
PshAI GACNNNNGTC 1 cut(s) 1584
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 2 cut(s) 54, 2181
Psp6I CCWGG 4 cut(s) 894, 1788, 1948, 2107
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 4 cut(s) 894, 1788, 1948, 2107
PspN4I GGNNCC 5 cut(s) 25, 321, 591, 1011, 1872
PspPI GGNCC 1 cut(s) 320
PspXI VCTCGAGB 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 1 cut(s) 1810
RsaI GTAC 3 cut(s) 1130, 1442, 2350
RsaNI GTAC 3 cut(s) 1129, 1441, 2349
RseI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
SacI GAGCTC 2 cut(s) 54, 2181
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 9 cut(s) 270, 405, 678, 797, 1272, 1454, 1538, 1694, 1917
SatI GCNGC 6 cut(s) 147, 294, 432, 446, 573, 919
Sau3AI GATC 7 cut(s) 508, 973, 1020, 1099, 1810, 2050, 2116
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 4 cut(s) 1187, 1420, 1546, 2219
ScrFI CCNGG 5 cut(s) 6, 896, 1790, 1950, 2109
SduI GDGCHC 5 cut(s) 54, 1014, 1994, 2181, 2247
SfaNI GCATC 8 cut(s) 152, 238, 505, 526, 628, 1825, 1840, 1862
Sfr274I CTCGAG 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 5 cut(s) 1092, 1320, 1483, 1520, 1805
SmlI CTYRAG 8 cut(s) 47, 785, 1385, 1693, 1703, 1769, 2030, 2123
SmoI CTYRAG 8 cut(s) 47, 785, 1385, 1693, 1703, 1769, 2030, 2123
SpeI ACTAGT 1 cut(s) 1546
SseBI AGGCCT 1 cut(s) 1212
SsiI CCGC 9 cut(s) 117, 129, 146, 149, 293, 448, 826, 1159, 2382
SspI AATATT 1 cut(s) 693
SspMI CTAG 3 cut(s) 1547, 1668, 1814
SstI GAGCTC 2 cut(s) 54, 2181
StuI AGGCCT 1 cut(s) 1212
StyD4I CCNGG 5 cut(s) 4, 894, 1788, 1948, 2107
StyI CCWWGG 3 cut(s) 870, 1154, 2214
TaiI ACGT 1 cut(s) 1588
TaqI TCGA 8 cut(s) 48, 169, 263, 885, 1543, 1744, 2181, 2310
TaqII GACCGA 1 cut(s) 2163
TatI WGTACW 1 cut(s) 1128
TauI GCSGC 2 cut(s) 149, 296
TfiI GAWTC 7 cut(s) 1379, 1467, 1662, 1922, 2167, 2203, 2318
Tru1I TTAA 9 cut(s) 270, 405, 678, 797, 1272, 1454, 1538, 1694, 1917
Tru9I TTAA 9 cut(s) 270, 405, 678, 797, 1272, 1454, 1538, 1694, 1917
TscAI CASTG 6 cut(s) 1039, 1071, 1373, 1522, 1602, 2093
TseFI GTSAC 5 cut(s) 112, 743, 1034, 1549, 2353
TseI GCWGC 4 cut(s) 431, 445, 572, 918
Tsp45I GTSAC 5 cut(s) 112, 743, 1034, 1549, 2353
TspDTI ATGAA 6 cut(s) 128, 291, 597, 713, 1160, 1259
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 6 cut(s) 1039, 1071, 1373, 1522, 1602, 2093
Vha464I CTTAAG 1 cut(s) 1693
VneI GTGCAC 1 cut(s) 1990
XagI CCTNNNNNAGG 1 cut(s) 2106
XapI RAATTY 3 cut(s) 328, 344, 1418
XbaI TCTAGA 1 cut(s) 1813
XceI RCATGY 3 cut(s) 485, 1258, 1279
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 1 cut(s) 47
XmnI GAANNNNTTC 3 cut(s) 588, 672, 2025
XspI CTAG 3 cut(s) 1547, 1668, 1814
Zsp2I ATGCAT 3 cut(s) 1093, 1855, 2236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.