Rh4CG358600

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000872
Physical Location & Seq
Forward (+)
61568369 .. 61584995
16627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG358600.1

Sequence Viewer

Length: 1341 bp
ATGCCGGCCAAGGATGACGCCGCCGCCGCCGAGAAGGAGAAGAACCTTGAGCTCTTCCTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACCATCGCCAACAACAAGGTCACGGCGAATCTAACGGCCGTCATTGACGAGGCTGGAGTTACTGATGGATGCAGTCGAAGCGTTGGTAATCTTCTATACACGGTTGCTACAAAGTTTCCGGCAAATGCCCTTGTGCATCGCCCCGTGTTAATACAGTACATTGTGTCTTCAAAGATTAAAATCCCGGCTCAGTTAGATGCGGCTTTGTCATATTTTGCGAAAACGGGGCCAGAAAACTTCAAAACAACTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAACAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCCTGGGCTGACCCAAAAATTGTCAAGGCAGCCATTGATACAAACTTGCGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTGTACTCCTGAACAACCAGCTGAAGAAATTCTAAATCCATATGCAATATTCCCTCAGCCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAGCACTTGAGTGTGACTGGGGGCAGAGTTTTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGACATCCCACACTACTACTCGATCTCCAAGCGAGCAGCTCAATCAATTGATTAATATGGAACTGATAGACAGTCTCTGTGAGTTGGAGAGTCTCGGCGCCAATCATCATCATGGAATCATGGCTCTCTCCACTGACAGCTTTACGCATCATCATCATCATGATCAGCAACAACCTCAAGTCCCTGCAGCTATGAATTTCAGCACTGATCATAGTCATGATATTCAGAGATTTTCTCATTCTCATGATCGAAATGATGATGATGATCATCATCATCATGTCCTACTTGCTCAATCAACAGCTGCAGATTTTGGAGAACAAAGCCAAATGGAAAAGCCATTGGGAGGAAGAAAGAGAAAGAGAAACAATACTGATAAAGATATGGACAAACCAAAAGAGGTCATTCATGTGAGAGCTAAGCGAGGCCAAGCTACTGATAGCCACAGTTTGGCAGAAAGGGTATTTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

446

Amino Acids

49.8

Weight (kDa)

6.29

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 14 - 171 2.7e-56 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 174 - 249 2.6e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 273 - 292 1.2e-07 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 968
AccB7I CCANNNNNTGG 1 cut(s) 1318
AccBSI CCGCTC 1 cut(s) 79
AciI CCGC 5 cut(s) 21, 24, 27, 79, 293
AcoI YGGCCR 3 cut(s) 6, 84, 129
AcsI RAATTY 3 cut(s) 344, 672, 1066
AcuI CTGAAG 3 cut(s) 80, 687, 714
AcyI GRCGYC 2 cut(s) 18, 969
AfaI GTAC 2 cut(s) 251, 649
AfeI AGCGCT 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 1214, 1318
AgsI TTSAA 6 cut(s) 264, 334, 350, 374, 419, 788
AjnI CCWGG 1 cut(s) 500
AleI CACNNNNGTG 1 cut(s) 798
AluBI AGCT 9 cut(s) 52, 635, 665, 910, 1011, 1061, 1172, 1286, 1301
AluI AGCT 9 cut(s) 52, 635, 665, 910, 1011, 1061, 1172, 1286, 1301
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 3 cut(s) 487, 950, 968
AlwNI CAGNNNCTG 1 cut(s) 948
Aor51HI AGCGCT 1 cut(s) 449
AoxI GGCC 6 cut(s) 6, 84, 129, 320, 862, 1294
ApeKI GCWGC 6 cut(s) 445, 527, 572, 907, 1058, 1172
ApoI RAATTY 3 cut(s) 344, 672, 1066
AseI ATTAAT 1 cut(s) 924
Asp700I GAANNNNTTC 2 cut(s) 588, 672
AspLEI GCGC 2 cut(s) 450, 971
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 479, 572
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 54
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 6 cut(s) 457, 539, 584, 919, 1070, 1159
BccI CCATC 3 cut(s) 104, 152, 740
BceAI ACGGC 4 cut(s) 99, 116, 132, 144
BciT130I CCWGG 1 cut(s) 502
BclI TGATCA 3 cut(s) 1033, 1078, 1135
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 3 cut(s) 487, 950, 968
BfmI CTRYAG 3 cut(s) 570, 1056, 1173
BfoI RGCGCY 2 cut(s) 451, 972
BlpI GCTNAGC 1 cut(s) 1287
Bme1390I CCNGG 2 cut(s) 278, 502
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 3 cut(s) 321, 591, 970
BmrFI CCNGG 2 cut(s) 278, 502
BmrI ACTGGG 1 cut(s) 816
BmsI GCATC 4 cut(s) 152, 238, 280, 1027
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 1090, 1122
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 1287
BpuEI CTTGAG 3 cut(s) 68, 817, 1032
BpuMI CCSGG 1 cut(s) 278
BsaBI GATNNNNATC 4 cut(s) 272, 1134, 1137, 1140
BsaHI GRCGYC 2 cut(s) 18, 969
BsaJI CCNNGG 3 cut(s) 9, 500, 501
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 2 cut(s) 1214, 1318
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 75, 811
Bse8I GATNNNNATC 4 cut(s) 272, 1134, 1137, 1140
BseBI CCWGG 1 cut(s) 502
BseDI CCNNGG 3 cut(s) 9, 500, 501
BseGI GGATG 5 cut(s) 19, 79, 167, 496, 875
BseJI GATNNNNATC 4 cut(s) 272, 1134, 1137, 1140
BseLI CCNNNNNNNGG 2 cut(s) 1214, 1318
BseMII CTCAG 3 cut(s) 296, 713, 749
BseNI ACTGG 2 cut(s) 75, 811
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 6 cut(s) 457, 539, 584, 919, 1070, 1159
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 6 cut(s) 8, 86, 131, 322, 864, 1296
BshNI GGYRCC 1 cut(s) 968
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 3 cut(s) 5, 212, 278
BslFI GGGAC 1 cut(s) 1037
BslI CCNNNNNNNGG 2 cut(s) 1214, 1318
BsmAI GTCTC 3 cut(s) 487, 950, 968
BsmFI GGGAC 1 cut(s) 1037
BsnI GGCC 6 cut(s) 8, 86, 131, 322, 864, 1296
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
Bsp1720I GCTNAGC 1 cut(s) 1287
BspACI CCGC 5 cut(s) 21, 24, 27, 79, 293
BspANI GGCC 6 cut(s) 8, 86, 131, 322, 864, 1296
BspCNI CTCAG 3 cut(s) 295, 712, 750
BspHI TCATGA 3 cut(s) 1030, 1087, 1114
BspLI GGNNCC 3 cut(s) 321, 591, 970
BspMAI CTGCAG 3 cut(s) 574, 1060, 1177
BspQI GCTCTTC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 75, 811
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 3 cut(s) 9, 500, 501
BssMI GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
BssNI GRCGYC 2 cut(s) 18, 969
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 4 cut(s) 196, 249, 944, 1316
Bst6I CTCTTC 2 cut(s) 59, 485
BstACI GRCGYC 2 cut(s) 18, 969
BstC8I GCNNGC 3 cut(s) 6, 615, 905
BstDEI CTNAG 5 cut(s) 282, 485, 699, 758, 1287
BstF5I GGATG 5 cut(s) 19, 79, 167, 496, 875
BstH2I RGCGCY 2 cut(s) 451, 972
BstHHI GCGC 2 cut(s) 450, 971
BstKTI GATC 5 cut(s) 896, 1036, 1081, 1120, 1138
BstMAI GTCTC 3 cut(s) 487, 950, 968
BstMBI GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 26, 171
BstNI CCWGG 1 cut(s) 502
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 276, 500
BstSFI CTRYAG 3 cut(s) 570, 1056, 1173
BstV1I GCAGC 6 cut(s) 457, 539, 584, 919, 1070, 1159
BstV2I GAAGAC 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 6 cut(s) 8, 86, 131, 322, 864, 1296
BtgZI GCGATG 2 cut(s) 82, 215
BtsCI GGATG 5 cut(s) 19, 79, 167, 496, 875
BtsIMutI CAGTG 2 cut(s) 1002, 1074
Cac8I GCNNGC 3 cut(s) 6, 615, 905
CaiI CAGNNNCTG 1 cut(s) 948
CciI TCATGA 3 cut(s) 1030, 1087, 1114
CfoI GCGC 2 cut(s) 450, 971
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 320
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 250, 648
CviAII CATG 9 cut(s) 357, 866, 983, 991, 1031, 1088, 1115, 1148, 1277
CviQI GTAC 2 cut(s) 250, 648
DdeI CTNAG 5 cut(s) 282, 485, 699, 758, 1287
DinI GGCGCC 1 cut(s) 970
DpnI GATC 5 cut(s) 895, 1035, 1080, 1119, 1137
DpnII GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
EaeI YGGCCR 3 cut(s) 6, 84, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 59, 485
EarI CTCTTC 2 cut(s) 59, 485
Ecl136II GAGCTC 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 851
Eco47III AGCGCT 1 cut(s) 449
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 3 cut(s) 80, 687, 714
EcoICRI GAGCTC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 500
EcoRV GATATC 1 cut(s) 851
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 970
EheI GGCGCC 1 cut(s) 970
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 9 cut(s) 360, 869, 986, 994, 1034, 1091, 1118, 1151, 1280
FaqI GGGAC 1 cut(s) 1037
FatI CATG 9 cut(s) 356, 865, 982, 990, 1030, 1087, 1114, 1147, 1276
FauNDI CATATG 1 cut(s) 685
FbaI TGATCA 3 cut(s) 1033, 1078, 1135
FokI GGATG 5 cut(s) 26, 86, 174, 483, 862
FriOI GRGCYC 1 cut(s) 54
GlaI GCGC 2 cut(s) 449, 970
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 2 cut(s) 451, 972
HaeIII GGCC 6 cut(s) 8, 86, 131, 322, 864, 1296
HapII CCGG 3 cut(s) 5, 212, 278
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 450, 971
Hin1I GRCGYC 2 cut(s) 18, 969
Hin1II CATG 9 cut(s) 360, 869, 986, 994, 1034, 1091, 1118, 1151, 1280
Hin6I GCGC 2 cut(s) 448, 969
HinP1I GCGC 2 cut(s) 448, 969
HinfI GANTC 3 cut(s) 121, 961, 987
HpaII CCGG 3 cut(s) 5, 212, 278
HphI GGTGA 2 cut(s) 479, 572
Hpy188I TCNGA 4 cut(s) 68, 382, 759, 1098
Hpy188III TCNNGA 6 cut(s) 58, 653, 836, 1031, 1088, 1115
HpyAV CCTTC 2 cut(s) 28, 55
HpyCH4III ACNGT 4 cut(s) 196, 249, 944, 1316
HpyCH4V TGCA 9 cut(s) 165, 229, 434, 479, 572, 689, 768, 1058, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 171
HpyF3I CTNAG 5 cut(s) 282, 485, 699, 758, 1287
Hsp92I GRCGYC 2 cut(s) 18, 969
Hsp92II CATG 9 cut(s) 360, 869, 986, 994, 1034, 1091, 1118, 1151, 1280
HspAI GCGC 2 cut(s) 448, 969
KasI GGCGCC 1 cut(s) 968
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 3 cut(s) 1033, 1078, 1135
Kzo9I GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 6 cut(s) 457, 539, 584, 919, 1070, 1159
LweI GCATC 4 cut(s) 152, 238, 280, 1027
MaeIII GTNAC 4 cut(s) 112, 151, 467, 802
MalI GATC 5 cut(s) 895, 1035, 1080, 1119, 1137
MbiI CCGCTC 1 cut(s) 79
MboI GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
MfeI CAATTG 1 cut(s) 917
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 7 cut(s) 344, 435, 516, 551, 672, 917, 1066
Mly113I GGCGCC 1 cut(s) 969
MlyI GAGTC 1 cut(s) 970
MmeI TCCRAC 2 cut(s) 46, 936
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 588, 672
MseI TTAA 6 cut(s) 242, 270, 405, 717, 821, 924
MslI CAYNNNNRTG 7 cut(s) 798, 981, 1029, 1086, 1113, 1146, 1277
MspA1I CMGCKG 2 cut(s) 665, 1172
MspI CCGG 3 cut(s) 5, 212, 278
MspR9I CCNGG 2 cut(s) 278, 502
MunI CAATTG 1 cut(s) 917
MvaI CCWGG 1 cut(s) 502
MwoI GCNNNNNNNGC 2 cut(s) 26, 171
NaeI GCCGGC 1 cut(s) 6
NarI GGCGCC 1 cut(s) 969
NciI CCSGG 1 cut(s) 278
NdeI CATATG 1 cut(s) 685
NdeII GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 9 cut(s) 360, 869, 986, 994, 1034, 1091, 1118, 1151, 1280
NlaIV GGNNCC 3 cut(s) 321, 591, 970
NmeAIII GCCGAG 2 cut(s) 55, 945
NmuCI GTSAC 3 cut(s) 112, 467, 802
NspI RCATGY 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 798
PagI TCATGA 3 cut(s) 1030, 1087, 1114
PasI CCCWGGG 1 cut(s) 501
PciSI GCTCTTC 1 cut(s) 59
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 588, 672
PfeI GAWTC 2 cut(s) 121, 987
PflMI CCANNNNNTGG 1 cut(s) 1318
PleI GAGTC 1 cut(s) 969
PluTI GGCGCC 1 cut(s) 972
PpsI GAGTC 1 cut(s) 969
PshBI ATTAAT 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 3 cut(s) 321, 591, 970
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 3 cut(s) 574, 1060, 1177
PstNI CAGNNNCTG 1 cut(s) 948
PvuII CAGCTG 2 cut(s) 665, 1172
RsaI GTAC 2 cut(s) 251, 649
RsaNI GTAC 2 cut(s) 250, 648
RseI CAYNNNNRTG 7 cut(s) 798, 981, 1029, 1086, 1113, 1146, 1277
SacI GAGCTC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 6 cut(s) 242, 270, 405, 717, 821, 924
Sau3AI GATC 5 cut(s) 893, 1033, 1078, 1117, 1135
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 1 cut(s) 970
ScrFI CCNGG 2 cut(s) 278, 502
SduI GDGCHC 1 cut(s) 54
SfaNI GCATC 4 cut(s) 152, 238, 280, 1027
SfcI CTRYAG 3 cut(s) 570, 1056, 1173
SfoI GGCGCC 1 cut(s) 970
SmiMI CAYNNNNRTG 7 cut(s) 798, 981, 1029, 1086, 1113, 1146, 1277
SmlI CTYRAG 3 cut(s) 47, 796, 1047
SmoI CTYRAG 3 cut(s) 47, 796, 1047
Sse9I AATT 7 cut(s) 344, 435, 516, 551, 672, 917, 1066
SsiI CCGC 5 cut(s) 21, 24, 27, 79, 293
SspDI GGCGCC 1 cut(s) 968
SspI AATATT 1 cut(s) 693
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 276, 500
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 4 cut(s) 196, 249, 944, 1316
TaqI TCGA 3 cut(s) 169, 892, 1120
TasI AATT 7 cut(s) 344, 435, 516, 551, 672, 917, 1066
TatI WGTACW 2 cut(s) 249, 647
TauI GCSGC 4 cut(s) 23, 26, 29, 296
TfiI GAWTC 2 cut(s) 121, 987
Tru1I TTAA 6 cut(s) 242, 270, 405, 717, 821, 924
Tru9I TTAA 6 cut(s) 242, 270, 405, 717, 821, 924
TscAI CASTG 2 cut(s) 1009, 1081
TseFI GTSAC 3 cut(s) 112, 467, 802
TseI GCWGC 6 cut(s) 445, 527, 572, 907, 1058, 1172
Tsp45I GTSAC 3 cut(s) 112, 467, 802
TspDTI ATGAA 4 cut(s) 597, 713, 1079, 1265
TspRI CASTG 2 cut(s) 1009, 1081
Van91I CCANNNNNTGG 1 cut(s) 1318
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 3 cut(s) 344, 672, 1066
XceI RCATGY 1 cut(s) 360
XmnI GAANNNNTTC 2 cut(s) 588, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.