Rw6G013110

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000872
Physical Location & Seq
Reverse (-)
24148402 .. 24155517
7116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013110.1

Sequence Viewer

Length: 2385 bp
ATGCCTGCCAAGGACGACAGCGCCTCCGACAAGGAGAAGAGCCTTGAGCTCTTCTTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACAATCGCTAACCAGAAGGTCTCCGCCAATCTAAGCGCGGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGGAGCGTCGGTAATCTTCTCTACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCCGTGTTAACACAGTACATTGTGTCTTCAAAGATTAAAATCCCAGCTCAGTTAGATGCGGCTCTGTCATTTTTTGCAAACACGGGGCCAGAAAACTTCAAAACAAGTGAATTTGAACAAGCATGTGGTGTTGGTGTTCAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAACAGCGCTATAAAACAAATGTGGGTTACTTATTTGCACATCTTAGGAAGAGACATCCATGGGCTGACCCCAAAATTGTCAAGGCATCCATTGATACAAAGTTGCATGAATTCCTTGGTGAAAGGACTGCAACAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAACCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTCTACCCCTGAACAACCAGCTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGCGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACCTAAGTGTGACTGGCGGAAGAGTTTTAACTCGCTTTCCTCCTGAACCAAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAAGAATATATTGATCATATTCAAGAGATTGTCAATTGGATGGGTTGGGGGCCCTACAAGATCACTTACACTAGTGATTACTTTCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCATGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCGCTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAGGCAACTCTAAGAATGAAACAAGACATGCAGAGCGATAATTTTAACATGTATGACCTTATTGCATATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAACTGGTGTATCTATCCCAGTTACGATTATGCTCACTGCATTGTGGATTCTCTTGAGAATATCACGCATTCGCTGTGCAGTTTAGAATTTGAGACACGCCGTGCTTCATACTACTGGTTATTGCATTCTCTGGGCCTATATCAACCACATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGTAAGTTAAATCGACTAGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTACGACGCTGGCTGGTTTACGACGTAGGGGTGTGACGTCGAGTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTACTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCTGTAAAGGTCGTCATTACCAACTGGAAAGATGGCCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCATGTCCTATCAGGTGTGTGGATGCAAAGGATGTTGTTTTAGCCGAGGATAATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGATCGATCCACTCAAAGTGGAAGTTCGGTTGTTTGACAGACTATTCAATTCAGAGAATCCTGCTGAGCTTGATAACTGGCTTTCTGATTTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCCCACTTGAGAAAGGTGTTGGTGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCCTGAGAAGCTTGTGTTCAATCGGACGGTCACACTGCGAGATAGCTACAAGAGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

794

Amino Acids

90.72

Weight (kDa)

6.75

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 13 - 170 3.4e-54 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 173 - 248 1.6e-06 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 272 - 576 4.7e-119 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 579 - 682 2.6e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 692 - 770 2.9e-15 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1625
Acc36I ACCTGC 1 cut(s) 619
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 2307
AccII CGCG 1 cut(s) 128
AccIII TCCGGA 2 cut(s) 943, 2047
AciI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1841
AclWI GGATC 5 cut(s) 1571, 1754, 2039, 2105, 2114
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 4 cut(s) 341, 548, 669, 1415
AcuI CTGAAG 2 cut(s) 684, 711
AcyI GRCGYC 1 cut(s) 1622
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 5 cut(s) 248, 1127, 1678, 1706, 1877
AfeI AGCGCT 2 cut(s) 446, 1886
AfiI CCNNNNNNNGG 5 cut(s) 868, 1156, 1322, 1804, 2102
AflIII ACRYGT 2 cut(s) 1272, 1477
AhdI GACNNNNNGTC 1 cut(s) 1502
AhlI ACTAGT 2 cut(s) 1028, 1543
AjnI CCWGG 3 cut(s) 891, 1933, 2101
AleI CACNNNNGTG 1 cut(s) 795
Alw21I GWGCWC 3 cut(s) 51, 1071, 1725
Alw26I GTCTC 5 cut(s) 115, 484, 1320, 1415, 2009
AlwI GGATC 5 cut(s) 1571, 1754, 2039, 2105, 2114
AlwNI CAGNNNCTG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 1700
Aor13HI TCCGGA 2 cut(s) 943, 2047
Aor51HI AGCGCT 2 cut(s) 446, 1886
ApaI GGGCCC 1 cut(s) 1011
ApeKI GCWGC 3 cut(s) 143, 915, 2077
ApoI RAATTY 4 cut(s) 341, 548, 669, 1415
AseI ATTAAT 1 cut(s) 1635
Asp700I GAANNNNTTC 2 cut(s) 585, 669
AspLEI GCGC 4 cut(s) 23, 128, 447, 1887
AspS9I GGNCC 5 cut(s) 317, 1007, 1008, 1462, 1827
AsuHPI GGTGA 3 cut(s) 569, 1654, 2279
AsuII TTCGAA 1 cut(s) 2283
AvaI CYCGRG 1 cut(s) 1700
BaeGI GKGCMC 1 cut(s) 1011
BaeI ACNNNNGTAYC 4 cut(s) 1321, 1354, 1948, 1981
BanII GRGCYC 4 cut(s) 51, 1011, 1071, 1725
BarI GAAGNNNNNNTAC 2 cut(s) 1660, 1692
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 249, 2063
Bbv12I GWGCWC 3 cut(s) 51, 1071, 1725
BbvI GCAGC 3 cut(s) 130, 902, 2064
BccI CCATC 5 cut(s) 149, 737, 991, 1559, 1787
BceAI ACGGC 2 cut(s) 96, 1413
BciT130I CCWGG 3 cut(s) 893, 1935, 2103
BclI TGATCA 2 cut(s) 970, 1096
BcoDI GTCTC 5 cut(s) 115, 484, 1320, 1415, 2009
BcuI ACTAGT 2 cut(s) 1028, 1543
BfaI CTAG 2 cut(s) 1029, 1544
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 3 cut(s) 24, 448, 1888
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 4 cut(s) 144, 291, 916, 2078
BlpI GCTNAGC 1 cut(s) 2169
BlsI GCNGC 4 cut(s) 145, 292, 917, 2079
Bme1390I CCNGG 3 cut(s) 893, 1935, 2103
BmeRI GACNNNNNGTC 1 cut(s) 1502
BmeT110I CYCGRG 1 cut(s) 1700
BmgT120I GGNCC 5 cut(s) 317, 1007, 1008, 1462, 1827
BmiI GGNNCC 5 cut(s) 318, 588, 1008, 1009, 1857
BmrFI CCNGG 3 cut(s) 893, 1935, 2103
BmrI ACTGGG 1 cut(s) 1341
BmsI GCATC 5 cut(s) 149, 235, 277, 533, 1972
BmuI ACTGGG 1 cut(s) 1341
BpiI GAAGAC 2 cut(s) 249, 2063
BplI GAGNNNNNCTC 2 cut(s) 1707, 1739
BpmI CTGGAG 1 cut(s) 1344
Bpu1102I GCTNAGC 1 cut(s) 2169
Bpu14I TTCGAA 1 cut(s) 2283
BpuEI CTTGAG 4 cut(s) 65, 1403, 2045, 2267
Bsa29I ATCGAT 1 cut(s) 2109
BsaBI GATNNNNATC 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 1622
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 8 cut(s) 9, 497, 553, 867, 1151, 1866, 2004, 2102
BsaWI WCCGGW 2 cut(s) 943, 2047
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 160, 190
Bsc4I CCNNNNNNNGG 5 cut(s) 868, 1156, 1322, 1804, 2102
Bse1I ACTGG 7 cut(s) 72, 808, 1337, 1347, 1448, 1790, 2186
Bse3DI GCAATG 1 cut(s) 879
Bse8I GATNNNNATC 1 cut(s) 269
BseAI TCCGGA 2 cut(s) 943, 2047
BseBI CCWGG 3 cut(s) 893, 1935, 2103
BseCI ATCGAT 1 cut(s) 2109
BseDI CCNNGG 8 cut(s) 9, 497, 553, 867, 1151, 1866, 2004, 2102
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 5 cut(s) 868, 1156, 1322, 1804, 2102
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 8 cut(s) 293, 746, 914, 1682, 1827, 1966, 2160, 2316
BseNI ACTGG 7 cut(s) 72, 808, 1337, 1347, 1448, 1790, 2186
BseRI GAGGAG 2 cut(s) 1299, 1728
BseSI GKGCMC 1 cut(s) 1011
BseXI GCAGC 3 cut(s) 130, 902, 2064
BseYI CCCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 1426
Bsh1236I CGCG 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 2109
BsiHKAI GWGCWC 3 cut(s) 51, 1071, 1725
BsiHKCI CYCGRG 1 cut(s) 1700
BsiSI CCGG 2 cut(s) 944, 2048
BslFI GGGAC 1 cut(s) 1172
BslI CCNNNNNNNGG 5 cut(s) 868, 1156, 1322, 1804, 2102
BsmAI GTCTC 5 cut(s) 115, 484, 1320, 1415, 2009
BsmFI GGGAC 1 cut(s) 1172
BsmI GAATGC 2 cut(s) 1396, 1453
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 1700
Bsp119I TTCGAA 1 cut(s) 2283
Bsp120I GGGCCC 1 cut(s) 1007
Bsp1286I GDGCHC 4 cut(s) 51, 1011, 1071, 1725
Bsp13I TCCGGA 2 cut(s) 943, 2047
Bsp1407I TGTACA 1 cut(s) 1875
Bsp143I GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
Bsp1720I GCTNAGC 1 cut(s) 2169
Bsp19I CCATGG 1 cut(s) 497
BspACI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1841
BspCNI CTCAG 8 cut(s) 292, 747, 915, 1683, 1828, 1965, 2161, 2317
BspDI ATCGAT 1 cut(s) 2109
BspEI TCCGGA 2 cut(s) 943, 2047
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 5 cut(s) 318, 588, 1008, 1009, 1857
BspMAI CTGCAG 1 cut(s) 148
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 5 cut(s) 1571, 1754, 2039, 2105, 2114
BspQI GCTCTTC 3 cut(s) 32, 56, 1713
BspT104I TTCGAA 1 cut(s) 2283
BspTNI GGTCTC 1 cut(s) 115
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 879
BsrGI TGTACA 1 cut(s) 1875
BsrI ACTGG 7 cut(s) 72, 808, 1337, 1347, 1448, 1790, 2186
BssECI CCNNGG 8 cut(s) 9, 497, 553, 867, 1151, 1866, 2004, 2102
BssMI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
BssNI GRCGYC 1 cut(s) 1622
BssSI CACGAG 1 cut(s) 137
BssT1I CCWWGG 6 cut(s) 9, 497, 553, 867, 1151, 1866
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 3 cut(s) 893, 1935, 2103
Bst4CI ACNGT 6 cut(s) 193, 246, 1516, 1811, 2020, 2350
Bst6I CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1713
BstACI GRCGYC 1 cut(s) 1622
BstAUI TGTACA 1 cut(s) 1875
BstBI TTCGAA 1 cut(s) 2283
BstC8I GCNNGC 2 cut(s) 6, 1596
BstDSI CCRYGG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 1802
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 3 cut(s) 24, 448, 1888
BstHHI GCGC 4 cut(s) 23, 128, 447, 1887
BstKTI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2109, 2113
BstMAI GTCTC 5 cut(s) 115, 484, 1320, 1415, 2009
BstMBI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 3 cut(s) 893, 1935, 2103
BstNSI RCATGY 6 cut(s) 357, 1255, 1276, 1322, 1481, 1965
BstSCI CCNGG 3 cut(s) 891, 1933, 2101
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 1011
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 3 cut(s) 130, 902, 2064
BstV2I GAAGAC 2 cut(s) 249, 2063
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 1 cut(s) 1045
Bsu15I ATCGAT 1 cut(s) 2109
BsuTUI ATCGAT 1 cut(s) 2109
BtgI CCRYGG 1 cut(s) 497
BtgZI GCGATG 2 cut(s) 212, 756
BtsI GCAGTG 3 cut(s) 1068, 1363, 2354
BtsIMutI CAGTG 4 cut(s) 1068, 1363, 1512, 2354
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 6, 1596
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 4 cut(s) 23, 128, 447, 1887
Cfr13I GGNCC 5 cut(s) 317, 1007, 1008, 1462, 1827
ClaI ATCGAT 1 cut(s) 2109
CseI GACGC 2 cut(s) 160, 1600
Csp6I GTAC 5 cut(s) 247, 1126, 1677, 1705, 1876
CviQI GTAC 5 cut(s) 247, 1126, 1677, 1705, 1876
DpnI GATC 9 cut(s) 972, 1019, 1047, 1098, 1578, 1761, 2046, 2108, 2112
DpnII GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
DraIII CACNNNGTG 1 cut(s) 1430
DriI GACNNNNNGTC 1 cut(s) 1502
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1713
Eam1105I GACNNNNNGTC 1 cut(s) 1502
EarI CTCTTC 6 cut(s) 32, 56, 411, 482, 805, 1713
EciI GGCGGA 3 cut(s) 103, 822, 1856
Ecl136II GAGCTC 3 cut(s) 49, 1069, 1723
Eco130I CCWWGG 6 cut(s) 9, 497, 553, 867, 1151, 1866
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 4 cut(s) 51, 1011, 1071, 1725
Eco31I GGTCTC 1 cut(s) 115
Eco32I GATATC 1 cut(s) 848
Eco47III AGCGCT 2 cut(s) 446, 1886
Eco53kI GAGCTC 3 cut(s) 49, 1069, 1723
Eco57I CTGAAG 2 cut(s) 684, 711
Eco88I CYCGRG 1 cut(s) 1700
EcoICRI GAGCTC 3 cut(s) 49, 1069, 1723
EcoNI CCTNNNNNAGG 1 cut(s) 1802
EcoO109I RGGNCCY 2 cut(s) 1007, 1008
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 891, 1933, 2101
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 6 cut(s) 9, 497, 553, 867, 1151, 1866
EcoT22I ATGCAT 1 cut(s) 2230
EcoT38I GRGCYC 4 cut(s) 51, 1011, 1071, 1725
ErhI CCWWGG 6 cut(s) 9, 497, 553, 867, 1151, 1866
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 1172
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 2 cut(s) 970, 1096
FblI GTMKAC 1 cut(s) 2307
Fnu4HI GCNGC 4 cut(s) 144, 291, 916, 2078
FriOI GRGCYC 4 cut(s) 51, 1011, 1071, 1725
Fsp4HI GCNGC 4 cut(s) 144, 291, 916, 2078
FspBI CTAG 2 cut(s) 1029, 1544
GlaI GCGC 4 cut(s) 22, 127, 446, 1886
GluI GCNGC 4 cut(s) 144, 291, 916, 2078
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 1344
HaeII RGCGCY 3 cut(s) 24, 448, 1888
HapII CCGG 2 cut(s) 944, 2048
HgaI GACGC 2 cut(s) 160, 1600
HhaI GCGC 4 cut(s) 23, 128, 447, 1887
Hin1I GRCGYC 1 cut(s) 1622
Hin6I GCGC 4 cut(s) 21, 126, 445, 1885
HinP1I GCGC 4 cut(s) 21, 126, 445, 1885
HincII GTYRAC 2 cut(s) 240, 2308
HindII GTYRAC 2 cut(s) 240, 2308
HindIII AAGCTT 1 cut(s) 2330
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 2 cut(s) 944, 2048
HphI GGTGA 3 cut(s) 569, 1654, 2279
Hpy166II GTNNAC 4 cut(s) 240, 1305, 1604, 2308
Hpy8I GTNNAC 4 cut(s) 240, 1305, 1604, 2308
Hpy99I CGWCG 4 cut(s) 176, 1594, 1611, 1627
HpyAV CCTTC 6 cut(s) 52, 100, 1071, 1214, 1219, 2106
HpyCH4III ACNGT 6 cut(s) 193, 246, 1516, 1811, 2020, 2350
HpyCH4IV ACGT 4 cut(s) 1092, 1609, 1622, 1640
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpySE526I ACGT 4 cut(s) 1092, 1609, 1622, 1640
Hsp92I GRCGYC 1 cut(s) 1622
HspAI GCGC 4 cut(s) 21, 126, 445, 1885
Kpn2I TCCGGA 2 cut(s) 943, 2047
Ksp22I TGATCA 2 cut(s) 970, 1096
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
LguI GCTCTTC 3 cut(s) 32, 56, 1713
LmnI GCTCC 5 cut(s) 168, 1066, 1741, 1892, 1936
Lsp1109I GCAGC 3 cut(s) 130, 902, 2064
LweI GCATC 5 cut(s) 149, 235, 277, 533, 1972
MaeI CTAG 2 cut(s) 1029, 1544
MaeII ACGT 4 cut(s) 1092, 1609, 1622, 1640
MaeIII GTNAC 9 cut(s) 148, 464, 799, 1349, 1508, 1546, 1618, 2267, 2350
MalI GATC 9 cut(s) 972, 1019, 1047, 1098, 1578, 1761, 2046, 2108, 2112
MbiI CCGCTC 1 cut(s) 76
MboI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 4 cut(s) 51, 1011, 1071, 1725
MlyI GAGTC 5 cut(s) 1184, 1702, 1853, 1901, 2314
MmeI TCCRAC 3 cut(s) 43, 51, 146
Mph1103I ATGCAT 1 cut(s) 2230
MroI TCCGGA 2 cut(s) 943, 2047
MroXI GAANNNNTTC 2 cut(s) 585, 669
MslI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 2 cut(s) 944, 2048
MspR9I CCNGG 3 cut(s) 893, 1935, 2103
MunI CAATTG 1 cut(s) 991
Mva1269I GAATGC 2 cut(s) 1396, 1453
MvaI CCWGG 3 cut(s) 893, 1935, 2103
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 168
NcoI CCATGG 1 cut(s) 497
NdeI CATATG 1 cut(s) 682
NdeII GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
NlaIV GGNNCC 5 cut(s) 318, 588, 1008, 1009, 1857
NmeAIII GCCGAG 1 cut(s) 2029
NmuCI GTSAC 5 cut(s) 799, 1546, 1618, 2267, 2350
NsiI ATGCAT 1 cut(s) 2230
NspI RCATGY 6 cut(s) 357, 1255, 1276, 1322, 1481, 1965
NspV TTCGAA 1 cut(s) 2283
OliI CACNNNNGTG 1 cut(s) 795
PaeR7I CTCGAG 1 cut(s) 1700
PceI AGGCCT 1 cut(s) 1209
PciI ACATGT 2 cut(s) 1272, 1477
PciSI GCTCTTC 3 cut(s) 32, 56, 1713
PctI GAATGC 2 cut(s) 1396, 1453
PdmI GAANNNNTTC 2 cut(s) 585, 669
PfeI GAWTC 6 cut(s) 1376, 1659, 1684, 1832, 2161, 2315
PflMI CCANNNNNTGG 1 cut(s) 1322
PfoI TCCNGGA 1 cut(s) 1933
PkrI GCNGC 4 cut(s) 145, 292, 917, 2079
PleI GAGTC 5 cut(s) 1183, 1701, 1852, 1901, 2314
PpsI GAGTC 5 cut(s) 1183, 1701, 1852, 1901, 2314
PscI ACATGT 2 cut(s) 1272, 1477
PshBI ATTAAT 1 cut(s) 1635
Psp124BI GAGCTC 3 cut(s) 51, 1071, 1725
Psp6I CCWGG 3 cut(s) 891, 1933, 2101
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 3 cut(s) 891, 1933, 2101
PspN4I GGNNCC 5 cut(s) 318, 588, 1008, 1009, 1857
PspOMI GGGCCC 1 cut(s) 1007
PspPI GGNCC 5 cut(s) 317, 1007, 1008, 1462, 1827
PspXI VCTCGAGB 1 cut(s) 1700
PstI CTGCAG 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 1045
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 5 cut(s) 248, 1127, 1678, 1706, 1877
RsaNI GTAC 5 cut(s) 247, 1126, 1677, 1705, 1876
RseI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
SacI GAGCTC 3 cut(s) 51, 1071, 1725
SalI GTCGAC 1 cut(s) 2306
SapI GCTCTTC 3 cut(s) 32, 56, 1713
SatI GCNGC 4 cut(s) 144, 291, 916, 2078
Sau3AI GATC 9 cut(s) 970, 1017, 1045, 1096, 1576, 1759, 2044, 2106, 2110
Sau96I GGNCC 5 cut(s) 317, 1007, 1008, 1462, 1827
SchI GAGTC 5 cut(s) 1184, 1702, 1853, 1901, 2314
ScrFI CCNGG 3 cut(s) 893, 1935, 2103
SduI GDGCHC 4 cut(s) 51, 1011, 1071, 1725
SfaNI GCATC 5 cut(s) 149, 235, 277, 533, 1972
SfcI CTRYAG 1 cut(s) 144
Sfr274I CTCGAG 1 cut(s) 1700
SfuI TTCGAA 1 cut(s) 2283
SlaI CTCGAG 1 cut(s) 1700
SmiMI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
SmlI CTYRAG 5 cut(s) 44, 1382, 1700, 2024, 2246
SmoI CTYRAG 5 cut(s) 44, 1382, 1700, 2024, 2246
SpeI ACTAGT 2 cut(s) 1028, 1543
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 6 cut(s) 76, 114, 128, 290, 807, 1841
SspI AATATT 1 cut(s) 1490
SspMI CTAG 2 cut(s) 1029, 1544
SstI GAGCTC 3 cut(s) 51, 1071, 1725
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 3 cut(s) 891, 1933, 2101
StyI CCWWGG 6 cut(s) 9, 497, 553, 867, 1151, 1866
TaaI ACNGT 6 cut(s) 193, 246, 1516, 1811, 2020, 2350
TaiI ACGT 4 cut(s) 1095, 1612, 1625, 1643
TatI WGTACW 4 cut(s) 246, 1125, 1676, 1875
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 6 cut(s) 1376, 1659, 1684, 1832, 2161, 2315
TscAI CASTG 4 cut(s) 1068, 1370, 1519, 2361
TseFI GTSAC 5 cut(s) 799, 1546, 1618, 2267, 2350
TseI GCWGC 3 cut(s) 143, 915, 2077
Tsp45I GTSAC 5 cut(s) 799, 1546, 1618, 2267, 2350
TspDTI ATGAA 8 cut(s) 561, 594, 710, 1068, 1157, 1256, 1425, 1676
TspRI CASTG 4 cut(s) 1068, 1370, 1519, 2361
Van91I CCANNNNNTGG 1 cut(s) 1322
VspI ATTAAT 1 cut(s) 1635
XagI CCTNNNNNAGG 1 cut(s) 1802
XapI RAATTY 4 cut(s) 341, 548, 669, 1415
XceI RCATGY 6 cut(s) 357, 1255, 1276, 1322, 1481, 1965
XhoI CTCGAG 1 cut(s) 1700
XmiI GTMKAC 1 cut(s) 2307
XmnI GAANNNNTTC 2 cut(s) 585, 669
XspI CTAG 2 cut(s) 1029, 1544
ZraI GACGTC 1 cut(s) 1623
Zsp2I ATGCAT 1 cut(s) 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.