RchiOBHm_Chr1g0324791

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000872
Physical Location & Seq
Forward (+)
12560166 .. 12561665
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55461

Sequence Viewer

Length: 1500 bp
ATGCCGGCCAAGGAGAAAACCCTTGAGCTCATGTTGAAGGTCGGACTGGACGAGCGGACGGCCAAAAACACAATGGCCAACAATAAGGTCACGTCCAATCTAAGGGCTGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGAAGCATTGGTAATCTTATATACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCGGTATTGGCACAGTACATTGTGTCTTCAAAGATTAAAACTCCAGCTCAGTTAGATGCGGCTTTGTTATTTTTTGCAAATACGGGGCCAGAAAACTTCAAAATAAGTGAATTTGAAGAAGCATGTGGAGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAATCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGGCTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGACACAAAGTTGGGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAACAAGAAAGAGAAACCTGCTAAAGTAGAGGAGAAAGCTACTGCTGAAGAAATTCTGAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGCTTGACTGGGGGGAGAGTTCTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGACTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAAGAATATATTGATCATATTCAAGAGATTGTCAGTTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTTCACGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACGGGGAATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCGCTAAAACTTTTTGAGGATATGAGGCGTGGCCTGATTGAAGAAGGGAAAGCAACTCTGAGAATGAAACAAGACATGCAGAATGATAATTTTAACATGTATGACCTTATTGCATATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAGTTGGTGTATCTACCCCAGTTACGACTATGCTCATTGCATAGTGGATTCTCTTGAGAATATCACTCATTCGCTGTGCACTTTAGAATTTGAGACACGGCGTGCTTCATACTACTGGTTGTTGCATTCTCTGGGCCTATATCAACCACATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAACGTAAGTTAAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

499

Amino Acids

57.34

Weight (kDa)

6.54

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 6 - 163 6e-54 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA-synt_1c PF00749 257 - 498 8.8e-103 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccBSI CCGCTC 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 898
AciI CCGC 2 cut(s) 55, 269
AcoI YGGCCR 3 cut(s) 6, 60, 75
AcsI RAATTY 3 cut(s) 320, 624, 1370
AcuI CTGAAG 2 cut(s) 639, 666
AdeI CACNNNGTG 1 cut(s) 1385
AfaI GTAC 2 cut(s) 227, 1082
AfeI AGCGCT 1 cut(s) 425
AfiI CCNNNNNNNGG 5 cut(s) 102, 217, 823, 1111, 1277
AflIII ACRYGT 2 cut(s) 1227, 1432
AhdI GACNNNNNGTC 1 cut(s) 1457
AhlI ACTAGT 1 cut(s) 983
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 6 cut(s) 28, 257, 611, 753, 905, 1024
AluI AGCT 6 cut(s) 28, 257, 611, 753, 905, 1024
Alw21I GWGCWC 3 cut(s) 30, 1026, 1364
Alw26I GTCTC 3 cut(s) 463, 1275, 1370
Alw44I GTGCAC 1 cut(s) 1360
AlwNI CAGNNNCTG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 898
Aor51HI AGCGCT 1 cut(s) 425
AoxI GGCC 9 cut(s) 6, 60, 75, 296, 814, 844, 1118, 1162, 1417
ApaLI GTGCAC 1 cut(s) 1360
ApeKI GCWGC 3 cut(s) 122, 421, 548
ApoI RAATTY 3 cut(s) 320, 624, 1370
Asp700I GAANNNNTTC 2 cut(s) 564, 624
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 296, 1417
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 536, 548, 878
BaeGI GKGCMC 1 cut(s) 1364
BalI TGGCCA 1 cut(s) 77
BanII GRGCYC 3 cut(s) 30, 966, 1026
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 30, 1026, 1364
BbvI GCAGC 3 cut(s) 109, 433, 560
BccI CCATC 3 cut(s) 128, 692, 946
BceAI ACGGC 2 cut(s) 75, 1397
BciT130I CCWGG 1 cut(s) 848
BclI TGATCA 2 cut(s) 925, 1051
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 3 cut(s) 463, 1275, 1370
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 2 cut(s) 123, 546
BfoI RGCGCY 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 4 cut(s) 123, 270, 422, 549
BlsI GCNGC 4 cut(s) 124, 271, 423, 550
Bme1390I CCNGG 2 cut(s) 212, 848
BmeRI GACNNNNNGTC 1 cut(s) 1457
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 296, 1417
BmiI GGNNCC 3 cut(s) 297, 567, 963
BmrFI CCNGG 2 cut(s) 212, 848
BmrI ACTGGG 2 cut(s) 768, 1296
BmsI GCATC 4 cut(s) 128, 214, 256, 512
BmuI ACTGGG 2 cut(s) 768, 1296
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 2 cut(s) 237, 1299
BpuEI CTTGAG 3 cut(s) 44, 769, 1358
BpuMI CCSGG 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 9, 476, 822, 1106
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 217, 823, 1111, 1277
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 4 cut(s) 51, 763, 1302, 1403
Bse3DI GCAATG 2 cut(s) 834, 1318
BseAI TCCGGA 1 cut(s) 898
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 4 cut(s) 9, 476, 822, 1106
BseGI GGATG 5 cut(s) 143, 472, 503, 957, 1267
BseLI CCNNNNNNNGG 5 cut(s) 102, 217, 823, 1111, 1277
BseMI GCAATG 2 cut(s) 834, 1318
BseMII CTCAG 4 cut(s) 272, 701, 869, 1181
BseNI ACTGG 4 cut(s) 51, 763, 1302, 1403
BseRI GAGGAG 2 cut(s) 617, 1254
BseSI GKGCMC 1 cut(s) 1364
BseXI GCAGC 3 cut(s) 109, 433, 560
BshFI GGCC 9 cut(s) 8, 62, 77, 298, 816, 846, 1120, 1164, 1419
BsiHKAI GWGCWC 3 cut(s) 30, 1026, 1364
BsiSI CCGG 3 cut(s) 5, 212, 899
BslFI GGGAC 1 cut(s) 1127
BslI CCNNNNNNNGG 5 cut(s) 102, 217, 823, 1111, 1277
BsmAI GTCTC 3 cut(s) 463, 1275, 1370
BsmFI GGGAC 1 cut(s) 1127
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 9 cut(s) 8, 62, 77, 298, 816, 846, 1120, 1164, 1419
Bsp1286I GDGCHC 4 cut(s) 30, 966, 1026, 1364
Bsp13I TCCGGA 1 cut(s) 898
Bsp143I GATC 4 cut(s) 925, 972, 1000, 1051
BspACI CCGC 2 cut(s) 55, 269
BspANI GGCC 9 cut(s) 8, 62, 77, 298, 816, 846, 1120, 1164, 1419
BspCNI CTCAG 4 cut(s) 271, 702, 870, 1182
BspEI TCCGGA 1 cut(s) 898
BspLI GGNNCC 3 cut(s) 297, 567, 963
BspMAI CTGCAG 2 cut(s) 127, 550
BspMI ACCTGC 1 cut(s) 598
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 2 cut(s) 834, 1318
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 4 cut(s) 51, 763, 1302, 1403
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 9, 476, 822, 1106
BssMI GATC 4 cut(s) 925, 972, 1000, 1051
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 3 cut(s) 9, 822, 1106
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 4 cut(s) 172, 225, 1286, 1471
Bst6I CTCTTC 2 cut(s) 390, 461
BstC8I GCNNGC 2 cut(s) 6, 1386
BstDEI CTNAG 6 cut(s) 101, 258, 461, 710, 878, 1190
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 5 cut(s) 143, 472, 503, 957, 1267
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 4 cut(s) 928, 975, 1003, 1054
BstMAI GTCTC 3 cut(s) 463, 1275, 1370
BstMBI GATC 4 cut(s) 925, 972, 1000, 1051
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 1 cut(s) 848
BstNSI RCATGY 5 cut(s) 336, 1210, 1231, 1277, 1436
BstSCI CCNGG 2 cut(s) 210, 846
BstSFI CTRYAG 2 cut(s) 123, 546
BstSLI GKGCMC 1 cut(s) 1364
BstV1I GCAGC 3 cut(s) 109, 433, 560
BstV2I GAAGAC 1 cut(s) 228
BstXI CCANNNNNNTGG 1 cut(s) 1437
BsuRI GGCC 9 cut(s) 8, 62, 77, 298, 816, 846, 1120, 1164, 1419
BtgI CCRYGG 1 cut(s) 476
BtgZI GCGATG 1 cut(s) 191
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 5 cut(s) 143, 472, 503, 957, 1267
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 1023, 1467
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 6, 1386
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 2 cut(s) 296, 1417
Csp6I GTAC 2 cut(s) 226, 1081
CviAII CATG 7 cut(s) 31, 333, 818, 1207, 1228, 1274, 1433
CviQI GTAC 2 cut(s) 226, 1081
DdeI CTNAG 6 cut(s) 101, 258, 461, 710, 878, 1190
DpnI GATC 4 cut(s) 927, 974, 1002, 1053
DpnII GATC 4 cut(s) 925, 972, 1000, 1051
DraIII CACNNNGTG 1 cut(s) 1385
DriI GACNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 3 cut(s) 6, 60, 75
Eam1104I CTCTTC 2 cut(s) 390, 461
Eam1105I GACNNNNNGTC 1 cut(s) 1457
EarI CTCTTC 2 cut(s) 390, 461
Ecl136II GAGCTC 2 cut(s) 28, 1024
Eco130I CCWWGG 3 cut(s) 9, 822, 1106
Eco24I GRGCYC 3 cut(s) 30, 966, 1026
Eco32I GATATC 1 cut(s) 803
Eco47III AGCGCT 1 cut(s) 425
Eco53kI GAGCTC 2 cut(s) 28, 1024
Eco57I CTGAAG 2 cut(s) 639, 666
EcoICRI GAGCTC 2 cut(s) 28, 1024
EcoRII CCWGG 1 cut(s) 846
EcoRV GATATC 1 cut(s) 803
EcoT14I CCWWGG 3 cut(s) 9, 822, 1106
EcoT38I GRGCYC 3 cut(s) 30, 966, 1026
ErhI CCWWGG 3 cut(s) 9, 822, 1106
FaeI CATG 7 cut(s) 34, 336, 821, 1210, 1231, 1277, 1436
FaqI GGGAC 1 cut(s) 1127
FatI CATG 7 cut(s) 30, 332, 817, 1206, 1227, 1273, 1432
FauNDI CATATG 1 cut(s) 637
FbaI TGATCA 2 cut(s) 925, 1051
Fnu4HI GCNGC 4 cut(s) 123, 270, 422, 549
FokI GGATG 5 cut(s) 150, 459, 490, 964, 1254
FriOI GRGCYC 3 cut(s) 30, 966, 1026
Fsp4HI GCNGC 4 cut(s) 123, 270, 422, 549
FspBI CTAG 1 cut(s) 984
GlaI GCGC 1 cut(s) 425
GluI GCNGC 4 cut(s) 123, 270, 422, 549
GsuI CTGGAG 2 cut(s) 237, 1299
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 9 cut(s) 8, 62, 77, 298, 816, 846, 1120, 1164, 1419
HapII CCGG 3 cut(s) 5, 212, 899
HhaI GCGC 1 cut(s) 426
Hin1II CATG 7 cut(s) 34, 336, 821, 1210, 1231, 1277, 1436
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HinfI GANTC 3 cut(s) 631, 1130, 1331
HpaII CCGG 3 cut(s) 5, 212, 899
HphI GGTGA 3 cut(s) 536, 548, 878
Hpy166II GTNNAC 2 cut(s) 1260, 1362
Hpy188I TCNGA 7 cut(s) 44, 358, 630, 711, 879, 1059, 1191
Hpy188III TCNNGA 7 cut(s) 116, 788, 899, 935, 998, 1337, 1449
Hpy8I GTNNAC 2 cut(s) 1260, 1362
HpyAV CCTTC 3 cut(s) 31, 1026, 1169
HpyCH4III ACNGT 4 cut(s) 172, 225, 1286, 1471
HpyCH4IV ACGT 3 cut(s) 92, 1047, 1483
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 6 cut(s) 101, 258, 461, 710, 878, 1190
HpySE526I ACGT 3 cut(s) 92, 1047, 1483
Hsp92II CATG 7 cut(s) 34, 336, 821, 1210, 1231, 1277, 1436
HspAI GCGC 1 cut(s) 424
Kpn2I TCCGGA 1 cut(s) 898
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 925, 1051
Kzo9I GATC 4 cut(s) 925, 972, 1000, 1051
LmnI GCTCC 3 cut(s) 418, 961, 1021
Lsp1109I GCAGC 3 cut(s) 109, 433, 560
LweI GCATC 4 cut(s) 128, 214, 256, 512
MaeI CTAG 1 cut(s) 984
MaeII ACGT 3 cut(s) 92, 1047, 1483
MaeIII GTNAC 6 cut(s) 88, 127, 866, 1037, 1304, 1463
MalI GATC 4 cut(s) 927, 974, 1002, 1053
MbiI CCGCTC 1 cut(s) 55
MboI GATC 4 cut(s) 925, 972, 1000, 1051
MboII GAAGA 8 cut(s) 228, 338, 371, 374, 407, 478, 632, 1184
MhlI GDGCHC 4 cut(s) 30, 966, 1026, 1364
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 9 cut(s) 320, 411, 492, 527, 624, 1010, 1122, 1219, 1370
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 1 cut(s) 1139
MmeI TCCRAC 2 cut(s) 22, 929
Mox20I TGGCCA 1 cut(s) 77
MroI TCCGGA 1 cut(s) 898
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 564, 624
MscI TGGCCA 1 cut(s) 77
MseI TTAA 7 cut(s) 246, 381, 669, 1125, 1224, 1490, 1498
MslI CAYNNNNRTG 2 cut(s) 1272, 1472
Msp20I TGGCCA 1 cut(s) 77
MspI CCGG 3 cut(s) 5, 212, 899
MspR9I CCNGG 2 cut(s) 212, 848
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 1 cut(s) 848
MwoI GCNNNNNNNGC 1 cut(s) 147
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 637
NdeII GATC 4 cut(s) 925, 972, 1000, 1051
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 7 cut(s) 34, 336, 821, 1210, 1231, 1277, 1436
NlaIV GGNNCC 3 cut(s) 297, 567, 963
NmuCI GTSAC 3 cut(s) 88, 866, 1037
NspI RCATGY 5 cut(s) 336, 1210, 1231, 1277, 1436
PciI ACATGT 2 cut(s) 1227, 1432
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 1408
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 564, 624
PfeI GAWTC 2 cut(s) 631, 1331
PflMI CCANNNNNTGG 1 cut(s) 1277
PkrI GCNGC 4 cut(s) 124, 271, 423, 550
PleI GAGTC 1 cut(s) 1138
PpsI GAGTC 1 cut(s) 1138
PscI ACATGT 2 cut(s) 1227, 1432
Psp124BI GAGCTC 2 cut(s) 30, 1026
Psp6I CCWGG 1 cut(s) 846
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 3 cut(s) 297, 567, 963
PspPI GGNCC 2 cut(s) 296, 1417
PstI CTGCAG 2 cut(s) 127, 550
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 227, 1082
RsaNI GTAC 2 cut(s) 226, 1081
RseI CAYNNNNRTG 2 cut(s) 1272, 1472
SacI GAGCTC 2 cut(s) 30, 1026
SaqAI TTAA 7 cut(s) 246, 381, 669, 1125, 1224, 1490, 1498
SatI GCNGC 4 cut(s) 123, 270, 422, 549
Sau3AI GATC 4 cut(s) 925, 972, 1000, 1051
Sau96I GGNCC 2 cut(s) 296, 1417
SchI GAGTC 1 cut(s) 1139
ScrFI CCNGG 2 cut(s) 212, 848
SduI GDGCHC 4 cut(s) 30, 966, 1026, 1364
SfaNI GCATC 4 cut(s) 128, 214, 256, 512
SfcI CTRYAG 2 cut(s) 123, 546
SmiMI CAYNNNNRTG 2 cut(s) 1272, 1472
SmlI CTYRAG 3 cut(s) 23, 748, 1337
SmoI CTYRAG 3 cut(s) 23, 748, 1337
SpeI ACTAGT 1 cut(s) 983
Sse9I AATT 9 cut(s) 320, 411, 492, 527, 624, 1010, 1122, 1219, 1370
SsiI CCGC 2 cut(s) 55, 269
SspI AATATT 1 cut(s) 1445
SspMI CTAG 1 cut(s) 984
SstI GAGCTC 2 cut(s) 30, 1026
StyD4I CCNGG 2 cut(s) 210, 846
StyI CCWWGG 3 cut(s) 9, 822, 1106
TaaI ACNGT 4 cut(s) 172, 225, 1286, 1471
TaiI ACGT 3 cut(s) 95, 1050, 1486
TaqI TCGA 2 cut(s) 145, 1478
TasI AATT 9 cut(s) 320, 411, 492, 527, 624, 1010, 1122, 1219, 1370
TatI WGTACW 2 cut(s) 225, 1080
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 2 cut(s) 631, 1331
Tru1I TTAA 7 cut(s) 246, 381, 669, 1125, 1224, 1490, 1498
Tru9I TTAA 7 cut(s) 246, 381, 669, 1125, 1224, 1490, 1498
TscAI CASTG 2 cut(s) 1023, 1474
TseFI GTSAC 3 cut(s) 88, 866, 1037
TseI GCWGC 3 cut(s) 122, 421, 548
Tsp45I GTSAC 3 cut(s) 88, 866, 1037
TspDTI ATGAA 6 cut(s) 573, 665, 1023, 1112, 1211, 1380
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 2 cut(s) 1023, 1474
Van91I CCANNNNNTGG 1 cut(s) 1277
VneI GTGCAC 1 cut(s) 1360
XapI RAATTY 3 cut(s) 320, 624, 1370
XceI RCATGY 5 cut(s) 336, 1210, 1231, 1277, 1436
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmnI GAANNNNTTC 2 cut(s) 564, 624
XspI CTAG 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.