Rorug01G0047700

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000872
Physical Location & Seq
Forward (+)
7902760 .. 7907007
4248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0047700.1

Sequence Viewer

Length: 3582 bp
ATGGCGTCAACCTCTTCTCGTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATAGCCGCCATATTTTTACCGGTCGCCTGTATGCTGCACTAACTCAGAGAGGAATTCTCACCTTTATGGATGAGCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGATTCTAGATCTGCGATTGTCATTCTCTCATGGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAAATGGGCCAACAAGTCCTCCCCGTCTTCTTAAACGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGCAAATTTTCACCTCCCCGAATGGGAACCCCAAGTAGAAGTAAGGGCACATGAAGAAGTTTATGGGAAGCTTAATGAGGACAGACTAAATGCCAGGAGAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGCATTCCAAGAATTATGTATCTGATTCAAAAATAATTGACGAAATTGTTAATCATATACTAAAGAAGTTCGTGTATACATCTTCAAGTTTTCACAAGGGCTTCGTAGGAATGGATTCCCGCATTGATGATTTGTTAAGCAATTACATATGCCCGCAGCTTTGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCATTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGCATTATTGACGTGCTTCGGATTAGTGTTGATGGACTGGATGAAAAAGATAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTTGACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTGGATGCATGATTTGCTACAAGAAATGGGATGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCGGGCAAACGTAGTAGACTATGGTCTCATGAAGACATCAACAATGTGCTGAAAAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAATCCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTCCTCCATATTCATAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAATTTTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCTACAGCAACATTAGACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAGTCCATCAATCCCTTAGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAAATTCCCCAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGAAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAACTGCAAAAATCTTGTCTGTCTTCCGAGCACCATCAATAAATTGAAGTCTATTAGAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCTCATAAAATATGTGAGAGGTTCAGTCTTTCGTGGATGCGAAGTAGTTTGGGGATCTGTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGGGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTTTGGCATTAGATTGCTTTCTAAGCTTGAGAACTTCAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAAGGCAGATGGTTGTACTTCACTGAAATACCTGTTTGATGCATCAAATTTGAACGGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCATATGGTAATTCCAGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTGGGTGTTCATTAAGTACGGGACCTGCATGCTTAGATGAAGTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCAGTGGATAAGCTTTATATGGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCGTGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAGTTTTGCCAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGGTAAATACTTTGGTACAGAGAGGTGGCGTAACTGTTACGGTCAGTTGGAGTTCTTATTTGAAACCAGAGGGCCGGGTGTGAAGGTGAGAGAGTGTGGAGTCCGTCTGATATATGAGGAAGATGTGCAAGAGTTGAACCGAACAACCACTTCATCAAGCAGTGGGATGTCTCCTTATGGGGATAGATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

1193

Amino Acids

135.25

Weight (kDa)

6.55

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 193 9.1e-44 TIR domain
TIR_2 PF13676 15 - 123 1.5e-14 TIR domain
NB-ARC PF00931 218 - 373 7.2e-19 NB-ARC domain
NACHT PF05729 223 - 319 9.9e-07 NACHT domain
WHD_ROQ1 PF23282 440 - 511 1.7e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 647 - 776 1.4e-08 Leucine-rich repeat region
LRR_13 PF23286 700 - 784 1.8e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 711 - 780 3.4e-08 Leucine-rich repeat region
C-JID PF20160 1003 - 1149 2.4e-12 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 761, 3082
AccB1I GGYRCC 1 cut(s) 348
AccB7I CCANNNNNTGG 2 cut(s) 1627, 3142
AccI GTMKAC 3 cut(s) 566, 1558, 2800
AciI CCGC 7 cut(s) 73, 346, 610, 644, 685, 892, 2563
AclI AACGTT 1 cut(s) 2173
AclWI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2506
AcuI CTGAAG 2 cut(s) 2972, 3227
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 2827, 3067, 3123, 3334
AfiI CCNNNNNNNGG 6 cut(s) 379, 1424, 1627, 2576, 3142, 3530
AflII CTTAAG 1 cut(s) 1789
AgeI ACCGGT 1 cut(s) 86
AjiI CACGTC 1 cut(s) 1291
AjnI CCWGG 4 cut(s) 449, 985, 3010, 3276
AjuI GAANNNNNNNTTGG 2 cut(s) 2732, 2764
Alw21I GWGCWC 2 cut(s) 2297, 3135
AlwI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2506
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 2 cut(s) 711, 2069
AoxI GGCC 7 cut(s) 170, 295, 793, 1735, 2242, 2549, 3389
ApeKI GCWGC 5 cut(s) 101, 185, 458, 646, 875
ArsI GACNNNNNNTTYG 2 cut(s) 2265, 2297
AseI ATTAAT 1 cut(s) 2114
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 2957, 2994
AspS9I GGNCC 6 cut(s) 295, 983, 1723, 2549, 3071, 3389
AsuC2I CCSGG 2 cut(s) 1545, 3393
AsuHPI GGTGA 6 cut(s) 118, 359, 2636, 2680, 3284, 3415
AvaI CYCGRG 2 cut(s) 711, 2069
AvaII GGWCC 3 cut(s) 983, 1723, 3071
BaeGI GKGCMC 1 cut(s) 406
BamHI GGATCC 2 cut(s) 331, 672
BanI GGYRCC 1 cut(s) 348
BauI CACGAG 2 cut(s) 1193, 3315
BbsI GAAGAC 5 cut(s) 307, 1581, 2279, 2696, 2960
Bbv12I GWGCWC 2 cut(s) 2297, 3135
BbvI GCAGC 5 cut(s) 88, 172, 445, 658, 862
BceAI ACGGC 2 cut(s) 340, 2249
BcgI CGANNNNNNTGC 2 cut(s) 2050, 2084
BciT130I CCWGG 4 cut(s) 451, 987, 3012, 3278
BciVI GTATCC 1 cut(s) 1794
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 2 cut(s) 1545, 3393
BfaI CTAG 4 cut(s) 201, 797, 1397, 2804
BfmI CTRYAG 1 cut(s) 1846
BfrI CTTAAG 1 cut(s) 1789
BfuAI ACCTGC 2 cut(s) 761, 3082
BfuI GTATCC 1 cut(s) 1794
BglII AGATCT 4 cut(s) 203, 1448, 1793, 1975
BisI GCNGC 7 cut(s) 73, 102, 186, 347, 459, 647, 876
BlsI GCNGC 7 cut(s) 74, 103, 187, 348, 460, 648, 877
Bme1390I CCNGG 6 cut(s) 451, 987, 1545, 3012, 3278, 3393
Bme18I GGWCC 3 cut(s) 983, 1723, 3071
BmeT110I CYCGRG 2 cut(s) 711, 2069
BmgBI CACGTC 1 cut(s) 1291
BmgT120I GGNCC 6 cut(s) 295, 983, 1723, 2549, 3071, 3389
BmiI GGNNCC 5 cut(s) 333, 350, 384, 674, 3072
BmrFI CCNGG 6 cut(s) 451, 987, 1545, 3012, 3278, 3393
BmrI ACTGGG 2 cut(s) 1788, 2146
BmsI GCATC 8 cut(s) 1036, 1153, 1473, 2330, 2471, 2839, 2861, 2915
BmuI ACTGGG 2 cut(s) 1788, 2146
BoxI GACNNNNGTC 2 cut(s) 470, 1564
BpiI GAAGAC 5 cut(s) 307, 1581, 2279, 2696, 2960
BplI GAGNNNNNCTC 2 cut(s) 108, 140
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 4 cut(s) 170, 1780, 1985, 2756
BpuMI CCSGG 2 cut(s) 1545, 3393
BsaBI GATNNNNATC 2 cut(s) 933, 1377
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1572, 1928
BsaJI CCNNGG 3 cut(s) 676, 986, 1621
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 4 cut(s) 291, 321, 1686, 1716
Bsc4I CCNNNNNNNGG 6 cut(s) 379, 1424, 1627, 2576, 3142, 3530
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 7 cut(s) 980, 1320, 1783, 2152, 3140, 3524, 3540
Bse8I GATNNNNATC 2 cut(s) 933, 1377
BseBI CCWGG 4 cut(s) 451, 987, 3012, 3278
BseDI CCNNGG 3 cut(s) 676, 986, 1621
BseJI GATNNNNATC 2 cut(s) 933, 1377
BseLI CCNNNNNNNGG 6 cut(s) 379, 1424, 1627, 2576, 3142, 3530
BseMII CTCAG 8 cut(s) 125, 459, 1187, 1711, 2228, 2252, 2597, 3018
BseNI ACTGG 7 cut(s) 980, 1320, 1783, 2152, 3140, 3524, 3540
BseRI GAGGAG 2 cut(s) 1694, 1757
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 5 cut(s) 88, 172, 445, 658, 862
BsgI GTGCAG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 7 cut(s) 172, 297, 795, 1737, 2244, 2551, 3391
BshNI GGYRCC 1 cut(s) 348
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 2297, 3135
BsiHKCI CYCGRG 2 cut(s) 711, 2069
BsiSI CCGG 3 cut(s) 87, 1544, 3392
BslFI GGGAC 2 cut(s) 1107, 3084
BslI CCNNNNNNNGG 6 cut(s) 379, 1424, 1627, 2576, 3142, 3530
BsmFI GGGAC 2 cut(s) 1107, 3084
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 7 cut(s) 172, 297, 795, 1737, 2244, 2551, 3391
Bso31I GGTCTC 2 cut(s) 1572, 1928
BsoBI CYCGRG 2 cut(s) 711, 2069
Bsp1286I GDGCHC 3 cut(s) 406, 2297, 3135
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 7 cut(s) 73, 346, 610, 644, 685, 892, 2563
BspANI GGCC 7 cut(s) 172, 297, 795, 1737, 2244, 2551, 3391
BspCNI CTCAG 8 cut(s) 124, 460, 1186, 1710, 2229, 2253, 2598, 3019
BspHI TCATGA 1 cut(s) 1570
BspLI GGNNCC 5 cut(s) 333, 350, 384, 674, 3072
BspMI ACCTGC 2 cut(s) 761, 3082
BspPI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2506
BspT107I GGYRCC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 1789
BspTNI GGTCTC 2 cut(s) 1572, 1928
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 7 cut(s) 980, 1320, 1783, 2152, 3140, 3524, 3540
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 676, 986, 1621
BssNAI GTATAC 2 cut(s) 567, 2801
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1193, 3315
BssT1I CCWWGG 2 cut(s) 676, 1621
Bst1107I GTATAC 2 cut(s) 567, 2801
Bst2BI CACGAG 2 cut(s) 1193, 3315
Bst2UI CCWGG 4 cut(s) 451, 987, 3012, 3278
Bst4CI ACNGT 6 cut(s) 1076, 1479, 1530, 2387, 3353, 3359
Bst6I CTCTTC 3 cut(s) 19, 3214, 3261
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1789
BstAPI GCANNNNNTGC 2 cut(s) 1492, 3561
BstDSI CCRYGG 1 cut(s) 676
BstEII GGTNACC 1 cut(s) 2668
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 7 cut(s) 455, 687, 1492, 2731, 3110, 3552, 3561
BstNI CCWGG 4 cut(s) 451, 987, 3012, 3278
BstNSI RCATGY 2 cut(s) 685, 3081
BstPAI GACNNNNGTC 2 cut(s) 470, 1564
BstPI GGTNACC 1 cut(s) 2668
BstSCI CCNGG 6 cut(s) 449, 985, 1543, 3010, 3276, 3391
BstSFI CTRYAG 1 cut(s) 1846
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 5 cut(s) 88, 172, 445, 658, 862
BstV2I GAAGAC 5 cut(s) 307, 1581, 2279, 2696, 2960
BstX2I RGATCY 7 cut(s) 203, 331, 672, 1448, 1793, 1975, 2498
BstXI CCANNNNNNTGG 1 cut(s) 2159
BstYI RGATCY 7 cut(s) 203, 331, 672, 1448, 1793, 1975, 2498
BstZ17I GTATAC 2 cut(s) 567, 2801
BsuI GTATCC 1 cut(s) 1794
BsuRI GGCC 7 cut(s) 172, 297, 795, 1737, 2244, 2551, 3391
BtgI CCRYGG 1 cut(s) 676
BtrI CACGTC 1 cut(s) 1291
BtsI GCAGTG 2 cut(s) 3484, 3553
BtsIMutI CAGTG 5 cut(s) 2831, 3147, 3484, 3547, 3553
BveI ACCTGC 2 cut(s) 761, 3082
CaiI CAGNNNCTG 1 cut(s) 875
CciI TCATGA 1 cut(s) 1570
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 6 cut(s) 295, 983, 1723, 2549, 3071, 3389
Csp6I GTAC 4 cut(s) 2826, 3066, 3122, 3333
CspAI ACCGGT 1 cut(s) 86
CviQI GTAC 4 cut(s) 2826, 3066, 3122, 3333
DraI TTTAAA 1 cut(s) 2811
Eam1104I CTCTTC 3 cut(s) 19, 3214, 3261
EarI CTCTTC 3 cut(s) 19, 3214, 3261
Eco130I CCWWGG 2 cut(s) 676, 1621
Eco147I AGGCCT 2 cut(s) 172, 1737
Eco31I GGTCTC 2 cut(s) 1572, 1928
Eco47I GGWCC 3 cut(s) 983, 1723, 3071
Eco57I CTGAAG 2 cut(s) 2972, 3227
Eco88I CYCGRG 2 cut(s) 711, 2069
Eco91I GGTNACC 1 cut(s) 2668
EcoO109I RGGNCCY 1 cut(s) 3071
EcoO65I GGTNACC 1 cut(s) 2668
EcoRI GAATTC 2 cut(s) 120, 3164
EcoRII CCWGG 4 cut(s) 449, 985, 3010, 3276
EcoT14I CCWWGG 2 cut(s) 676, 1621
EcoT22I ATGCAT 3 cut(s) 284, 1488, 2854
ErhI CCWWGG 2 cut(s) 676, 1621
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 1107, 3084
FauI CCCGC 3 cut(s) 617, 651, 678
FauNDI CATATG 2 cut(s) 638, 3000
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 3 cut(s) 566, 1558, 2800
Fnu4HI GCNGC 7 cut(s) 73, 102, 186, 347, 459, 647, 876
Fsp4HI GCNGC 7 cut(s) 73, 102, 186, 347, 459, 647, 876
FspBI CTAG 4 cut(s) 201, 797, 1397, 2804
GluI GCNGC 7 cut(s) 73, 102, 186, 347, 459, 647, 876
HaeIII GGCC 7 cut(s) 172, 297, 795, 1737, 2244, 2551, 3391
HapII CCGG 3 cut(s) 87, 1544, 3392
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 9, 942, 1641
HindII GTYRAC 3 cut(s) 9, 942, 1641
HindIII AAGCTT 4 cut(s) 425, 2033, 2732, 3149
HpaII CCGG 3 cut(s) 87, 1544, 3392
HphI GGTGA 6 cut(s) 118, 359, 2636, 2680, 3284, 3415
Hpy166II GTNNAC 8 cut(s) 9, 567, 942, 1526, 1559, 1641, 1723, 2801
Hpy8I GTNNAC 8 cut(s) 9, 567, 942, 1526, 1559, 1641, 1723, 2801
Hpy99I CGWCG 2 cut(s) 24, 27
HpyCH4III ACNGT 6 cut(s) 1076, 1479, 1530, 2387, 3353, 3359
HpyCH4IV ACGT 3 cut(s) 1290, 1552, 2173
HpyF10VI GCNNNNNNNGC 7 cut(s) 455, 687, 1492, 2731, 3110, 3552, 3561
HpySE526I ACGT 3 cut(s) 1290, 1552, 2173
Hsp92I GRCGYC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 724
LmnI GCTCC 2 cut(s) 872, 2630
Lsp1109I GCAGC 5 cut(s) 88, 172, 445, 658, 862
LweI GCATC 8 cut(s) 1036, 1153, 1473, 2330, 2471, 2839, 2861, 2915
MaeI CTAG 4 cut(s) 201, 797, 1397, 2804
MaeII ACGT 3 cut(s) 1290, 1552, 2173
MaeIII GTNAC 5 cut(s) 1384, 2651, 2668, 3347, 3353
MfeI CAATTG 3 cut(s) 189, 2226, 2896
MflI RGATCY 7 cut(s) 203, 331, 672, 1448, 1793, 1975, 2498
MhlI GDGCHC 3 cut(s) 406, 2297, 3135
MlyI GAGTC 4 cut(s) 1758, 2013, 2083, 3426
MmeI TCCRAC 3 cut(s) 1491, 2140, 3345
Mph1103I ATGCAT 3 cut(s) 284, 1488, 2854
MroXI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 2957, 2994
MslI CAYNNNNRTG 3 cut(s) 131, 1631, 3045
MspCI CTTAAG 1 cut(s) 1789
MspI CCGG 3 cut(s) 87, 1544, 3392
MspR9I CCNGG 6 cut(s) 451, 987, 1545, 3012, 3278, 3393
MunI CAATTG 3 cut(s) 189, 2226, 2896
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 4 cut(s) 451, 987, 3012, 3278
MwoI GCNNNNNNNGC 7 cut(s) 455, 687, 1492, 2731, 3110, 3552, 3561
NciI CCSGG 2 cut(s) 1545, 3393
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 2 cut(s) 638, 3000
NlaIV GGNNCC 5 cut(s) 333, 350, 384, 674, 3072
NmuCI GTSAC 2 cut(s) 1384, 2668
NsiI ATGCAT 3 cut(s) 284, 1488, 2854
NspI RCATGY 2 cut(s) 685, 3081
PaeI GCATGC 2 cut(s) 685, 3081
PaeR7I CTCGAG 2 cut(s) 711, 2069
PagI TCATGA 1 cut(s) 1570
PceI AGGCCT 2 cut(s) 172, 1737
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 2957, 2994
PflFI GACNNNGTC 1 cut(s) 1418
PflMI CCANNNNNTGG 2 cut(s) 1627, 3142
PfoI TCCNGGA 1 cut(s) 3010
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 7 cut(s) 74, 103, 187, 348, 460, 648, 877
PleI GAGTC 4 cut(s) 1758, 2012, 2082, 3425
PpsI GAGTC 4 cut(s) 1758, 2012, 2082, 3425
PpuMI RGGWCCY 1 cut(s) 3071
PshAI GACNNNNGTC 2 cut(s) 470, 1564
PshBI ATTAAT 1 cut(s) 2114
Psp1406I AACGTT 1 cut(s) 2173
Psp5II RGGWCCY 1 cut(s) 3071
Psp6I CCWGG 4 cut(s) 449, 985, 3010, 3276
PspEI GGTNACC 1 cut(s) 2668
PspGI CCWGG 4 cut(s) 449, 985, 3010, 3276
PspN4I GGNNCC 5 cut(s) 333, 350, 384, 674, 3072
PspPI GGNCC 6 cut(s) 295, 983, 1723, 2549, 3071, 3389
PspPPI RGGWCCY 1 cut(s) 3071
PspXI VCTCGAGB 1 cut(s) 711
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 7 cut(s) 203, 331, 672, 1448, 1793, 1975, 2498
PsyI GACNNNGTC 1 cut(s) 1418
RsaI GTAC 4 cut(s) 2827, 3067, 3123, 3334
RsaNI GTAC 4 cut(s) 2826, 3066, 3122, 3333
RseI CAYNNNNRTG 3 cut(s) 131, 1631, 3045
SatI GCNGC 7 cut(s) 73, 102, 186, 347, 459, 647, 876
Sau96I GGNCC 6 cut(s) 295, 983, 1723, 2549, 3071, 3389
SchI GAGTC 4 cut(s) 1758, 2013, 2083, 3426
ScrFI CCNGG 6 cut(s) 451, 987, 1545, 3012, 3278, 3393
SduI GDGCHC 3 cut(s) 406, 2297, 3135
SfaNI GCATC 8 cut(s) 1036, 1153, 1473, 2330, 2471, 2839, 2861, 2915
SfcI CTRYAG 1 cut(s) 1846
Sfr274I CTCGAG 2 cut(s) 711, 2069
SinI GGWCC 3 cut(s) 983, 1723, 3071
SlaI CTCGAG 2 cut(s) 711, 2069
SmiMI CAYNNNNRTG 3 cut(s) 131, 1631, 3045
SmlI CTYRAG 7 cut(s) 149, 711, 1759, 1789, 1964, 2069, 2735
SmoI CTYRAG 7 cut(s) 149, 711, 1759, 1789, 1964, 2069, 2735
SphI GCATGC 2 cut(s) 685, 3081
SseBI AGGCCT 2 cut(s) 172, 1737
SsiI CCGC 7 cut(s) 73, 346, 610, 644, 685, 892, 2563
SspI AATATT 2 cut(s) 1338, 3022
SspMI CTAG 4 cut(s) 201, 797, 1397, 2804
StuI AGGCCT 2 cut(s) 172, 1737
StyD4I CCNGG 6 cut(s) 449, 985, 1543, 3010, 3276, 3391
StyI CCWWGG 2 cut(s) 676, 1621
TaaI ACNGT 6 cut(s) 1076, 1479, 1530, 2387, 3353, 3359
TaiI ACGT 3 cut(s) 1293, 1555, 2176
TaqI TCGA 3 cut(s) 712, 1770, 2070
TaqII GACCGA 1 cut(s) 762
TatI WGTACW 2 cut(s) 2825, 3121
TauI GCSGC 2 cut(s) 75, 349
TscAI CASTG 5 cut(s) 2838, 3147, 3484, 3547, 3553
TseFI GTSAC 2 cut(s) 1384, 2668
TseI GCWGC 5 cut(s) 101, 185, 458, 646, 875
Tsp45I GTSAC 2 cut(s) 1384, 2668
TspGWI ACGGA 3 cut(s) 1173, 2880, 3410
TspRI CASTG 5 cut(s) 2838, 3147, 3484, 3547, 3553
Tth111I GACNNNGTC 1 cut(s) 1418
Van91I CCANNNNNTGG 2 cut(s) 1627, 3142
Vha464I CTTAAG 1 cut(s) 1789
VpaK11BI GGWCC 3 cut(s) 983, 1723, 3071
VspI ATTAAT 1 cut(s) 2114
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 2 cut(s) 685, 3081
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 3047
XhoI CTCGAG 2 cut(s) 711, 2069
XmiI GTMKAC 3 cut(s) 566, 1558, 2800
XmnI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 2957, 2994
XspI CTAG 4 cut(s) 201, 797, 1397, 2804
Zsp2I ATGCAT 3 cut(s) 284, 1488, 2854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.