Rw6G013490

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000872
Physical Location & Seq
Forward (+)
24964379 .. 24970285
5907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013490.1

Sequence Viewer

Length: 2163 bp
ATGCCGGCCAAGGCCTCCGAGAAGGAGAAGAATAACCTTGAGCTCTTCTTGAAGGTCGACGGACTGGATGAGCGGACGGCCAAACACACAATCGCCAACAACAACGTCACCGCCGTCATTCACGAGGCTGCAGTTACTCATGGATGCAGTCGAAGCATTGGTAATCAATCTTCTATACACGATTTTTTTTCATTATTGGTGCCATACCATCTGTGGCAGGTTGCTAAAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCCGTATTGGCACACTACATTGTGTCTTCAAAGATTAAATTCCCAGCTCAGTTAGATGCGGCTTTGTCATTTTTTGCAAGTACGGGGCCAGAAAAATTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTGTTGAAGTTTCAGAGGAAGAAATCAAGCAATCTGTTAAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGCGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAGAAGTTGTCAAGTTGCATGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGCCAGTTGGAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGTGTGACTCGCTTTCCTCCTGAGCTGAATGGATATATACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCCATCTGAGGTTTGATGACACAAATCACGAAGCTGAAAAGAAGGAATATATTTATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCATGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCGAGGCCTGATTGAAGAAGGGAAAGCAACTCTAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTTATTGCATATCGTATCAAGTTTACTCCTCATCCGCATGCTGGAGACAAGTGGTCCACAAGTTACAATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATTTACCCATTTGAATTTGAGACACATCGTGGTTCATACTACTGGCTATTGCATTCTCTGGGCCTATATCAACCACATGTTGGGGAATATTCAAGACTGAATGTCAGTAACACTATTATGTCGAAGCGTAAGGTGAGTCACCTATTGGACGTACTTATGGGCTTGACGTCGACTGCCACTAATACGTTTGTTAGAGAAATTGGAATCACCAGAAGTGATTTTAGTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCTTGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCTGACTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCACTCCAATTTTCGCCTAAAGACTCAAAATACTACTTCGGACTTGCTCCTGGAGAATCTGCCCTGCTCAGTAACAAACTTTATCTTCATGGTCGCAGACACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACAGGTCGAGTATGATCAGGAGAAGAAGACAAAGCCAAAGCTTGATAACTGGCTTTCTGATCTGAATGGAAAATTGAAAGTGGTAATACCAAATGCATTTGCTGTCCCCCCCCCCCCCCCCCCCAAATTGGGAAAGGTGTTGGTGTTGGTGAGTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTGACTCCTGAGAAGCTTGTGTTTAATACGGTCACACTGCGAGATATCTACAAGAATGGGAAGTACGTAGGTCATCTTTGGAATCGTCAGAATAGAGAGGAATGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

720

Amino Acids

83.2

Weight (kDa)

6.9

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 13 - 178 1.2e-28 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA-synt_1c PF00749 245 - 491 2.4e-71 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 514 - 570 9.2e-10 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1793
AatII GACGTC 1 cut(s) 1430
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 199
AccB7I CCANNNNNTGG 1 cut(s) 1340
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 2 cut(s) 57, 1430
AciI CCGC 6 cut(s) 73, 111, 317, 485, 1193, 1646
AcoI YGGCCR 2 cut(s) 6, 78
AcsI RAATTY 5 cut(s) 296, 353, 368, 603, 1274
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 1427
AdeI CACNNNGTG 1 cut(s) 1289
AfaI GTAC 6 cut(s) 340, 1043, 1413, 1511, 1682, 2114
AfeI AGCGCT 1 cut(s) 470
AfiI CCNNNNNNNGG 8 cut(s) 265, 784, 1072, 1199, 1340, 1609, 1990, 1991
AflIII ACRYGT 2 cut(s) 1149, 1336
AhdI GACNNNNNGTC 2 cut(s) 1210, 1361
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 2 cut(s) 807, 1737
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AleI CACNNNNGTG 1 cut(s) 729
Alw21I GWGCWC 4 cut(s) 45, 987, 1530, 1692
Alw26I GTCTC 4 cut(s) 508, 1197, 1274, 1841
Ama87I CYCGRG 1 cut(s) 1505
Aor51HI AGCGCT 1 cut(s) 470
AoxI GGCC 9 cut(s) 6, 12, 78, 344, 775, 805, 1084, 1321, 1631
ApeKI GCWGC 4 cut(s) 128, 466, 575, 831
ApoI RAATTY 5 cut(s) 296, 353, 368, 603, 1274
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 3 cut(s) 344, 1212, 1321
AsuHPI GGTGA 8 cut(s) 100, 500, 575, 1391, 1405, 1459, 2023, 2030
AsuII TTCGAA 1 cut(s) 2034
AvaI CYCGRG 1 cut(s) 1505
AvaII GGWCC 1 cut(s) 1212
BanI GGYRCC 1 cut(s) 199
BanII GRGCYC 4 cut(s) 45, 927, 987, 1530
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 2 cut(s) 276, 1895
Bbv12I GWGCWC 4 cut(s) 45, 987, 1530, 1692
BbvCI CCTCAGC 1 cut(s) 1836
BbvI GCAGC 4 cut(s) 115, 478, 587, 818
BccI CCATC 4 cut(s) 216, 671, 843, 907
BceAI ACGGC 2 cut(s) 93, 98
BciT130I CCWGG 2 cut(s) 809, 1739
BclI TGATCA 2 cut(s) 1012, 1876
BcoDI GTCTC 4 cut(s) 508, 1197, 1274, 1841
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 2 cut(s) 129, 573
BfoI RGCGCY 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 208
BglII AGATCT 1 cut(s) 961
BisI GCNGC 5 cut(s) 129, 318, 467, 576, 832
BlsI GCNGC 5 cut(s) 130, 319, 468, 577, 833
Bme1390I CCNGG 2 cut(s) 809, 1739
Bme18I GGWCC 1 cut(s) 1212
BmeRI GACNNNNNGTC 2 cut(s) 1210, 1361
BmeT110I CYCGRG 1 cut(s) 1505
BmgT120I GGNCC 3 cut(s) 344, 1212, 1321
BmiI GGNNCC 4 cut(s) 201, 345, 924, 1662
BmrFI CCNGG 2 cut(s) 809, 1739
BmsI GCATC 4 cut(s) 134, 262, 304, 1803
BpiI GAAGAC 2 cut(s) 276, 1895
BplI GAGNNNNNCTC 4 cut(s) 721, 753, 1512, 1544
BpmI CTGGAG 2 cut(s) 1221, 1760
Bpu10I CCTNAGC 2 cut(s) 750, 1836
Bpu14I TTCGAA 1 cut(s) 2034
BpuEI CTTGAG 4 cut(s) 59, 748, 1274, 1877
BsaAI YACGTR 1 cut(s) 2116
BsaHI GRCGYC 1 cut(s) 1427
BsaJI CCNNGG 5 cut(s) 9, 521, 783, 1067, 1671
Bsc4I CCNNNNNNNGG 8 cut(s) 265, 784, 1072, 1199, 1340, 1609, 1990, 1991
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 5 cut(s) 69, 593, 1307, 1595, 1916
Bse3DI GCAATG 2 cut(s) 795, 1234
BseBI CCWGG 2 cut(s) 809, 1739
BseDI CCNNGG 5 cut(s) 9, 521, 783, 1067, 1671
BseGI GGATG 8 cut(s) 73, 149, 517, 918, 1033, 1189, 1818, 1827
BseLI CCNNNNNNNGG 8 cut(s) 265, 784, 1072, 1199, 1340, 1609, 1990, 1991
BseMI GCAATG 2 cut(s) 795, 1234
BseMII CTCAG 9 cut(s) 320, 680, 741, 830, 1487, 1632, 1770, 1827, 2049
BseNI ACTGG 5 cut(s) 69, 593, 1307, 1595, 1916
BseRI GAGGAG 2 cut(s) 1176, 1533
BseXI GCAGC 4 cut(s) 115, 478, 587, 818
BseYI CCCAGC 1 cut(s) 301
BshFI GGCC 9 cut(s) 8, 14, 80, 346, 777, 807, 1086, 1323, 1633
BshNI GGYRCC 1 cut(s) 199
BsiHKAI GWGCWC 4 cut(s) 45, 987, 1530, 1692
BsiHKCI CYCGRG 1 cut(s) 1505
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 2 cut(s) 1088, 1952
BslI CCNNNNNNNGG 8 cut(s) 265, 784, 1072, 1199, 1340, 1609, 1990, 1991
BsmAI GTCTC 4 cut(s) 508, 1197, 1274, 1841
BsmFI GGGAC 2 cut(s) 1088, 1952
BsmI GAATGC 1 cut(s) 1312
BsnI GGCC 9 cut(s) 8, 14, 80, 346, 777, 807, 1086, 1323, 1633
BsoBI CYCGRG 1 cut(s) 1505
Bsp119I TTCGAA 1 cut(s) 2034
Bsp1286I GDGCHC 5 cut(s) 45, 927, 987, 1530, 1692
Bsp1407I TGTACA 1 cut(s) 1680
Bsp143I GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
BspACI CCGC 6 cut(s) 73, 111, 317, 485, 1193, 1646
BspANI GGCC 9 cut(s) 8, 14, 80, 346, 777, 807, 1086, 1323, 1633
BspCNI CTCAG 9 cut(s) 319, 681, 742, 831, 1488, 1633, 1769, 1828, 2050
BspLI GGNNCC 4 cut(s) 201, 345, 924, 1662
BspMAI CTGCAG 2 cut(s) 133, 577
BspMI ACCTGC 1 cut(s) 208
BspQI GCTCTTC 2 cut(s) 50, 1518
BspT104I TTCGAA 1 cut(s) 2034
BspT107I GGYRCC 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 2 cut(s) 795, 1234
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 1680
BsrI ACTGG 5 cut(s) 69, 593, 1307, 1595, 1916
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 5 cut(s) 9, 521, 783, 1067, 1671
BssMI GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
BssNI GRCGYC 1 cut(s) 1427
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 4 cut(s) 9, 783, 1067, 1671
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 809, 1739
Bst4CI ACNGT 3 cut(s) 1616, 1852, 2080
Bst6I CTCTTC 4 cut(s) 50, 435, 506, 1518
BstACI GRCGYC 1 cut(s) 1427
BstAUI TGTACA 1 cut(s) 1680
BstBAI YACGTR 1 cut(s) 2116
BstBI TTCGAA 1 cut(s) 2034
BstC8I GCNNGC 2 cut(s) 6, 1197
BstDSI CCRYGG 1 cut(s) 521
BstENI CCTNNNNNAGG 1 cut(s) 1607
BstF5I GGATG 8 cut(s) 73, 149, 517, 918, 1033, 1189, 1818, 1827
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 6 cut(s) 936, 964, 1015, 1567, 1879, 1924
BstMAI GTCTC 4 cut(s) 508, 1197, 1274, 1841
BstMBI GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstNI CCWGG 2 cut(s) 809, 1739
BstNSI RCATGY 5 cut(s) 384, 1153, 1199, 1340, 1796
BstSCI CCNGG 2 cut(s) 807, 1737
BstSFI CTRYAG 2 cut(s) 129, 573
BstSNI TACGTA 1 cut(s) 2116
BstV1I GCAGC 4 cut(s) 115, 478, 587, 818
BstV2I GAAGAC 2 cut(s) 276, 1895
BstX2I RGATCY 1 cut(s) 961
BstYI RGATCY 1 cut(s) 961
BsuRI GGCC 9 cut(s) 8, 14, 80, 346, 777, 807, 1086, 1323, 1633
BtgI CCRYGG 1 cut(s) 521
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 8 cut(s) 73, 149, 517, 918, 1033, 1189, 1818, 1827
BtsI GCAGTG 2 cut(s) 984, 2084
BtsIMutI CAGTG 2 cut(s) 984, 2084
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 6, 1197
CfoI GCGC 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 344, 1212, 1321
Csp6I GTAC 6 cut(s) 339, 1042, 1412, 1510, 1681, 2113
CviQI GTAC 6 cut(s) 339, 1042, 1412, 1510, 1681, 2113
DpnI GATC 6 cut(s) 935, 963, 1014, 1566, 1878, 1923
DpnII GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
DraIII CACNNNGTG 1 cut(s) 1289
DrdI GACNNNNNNGTC 1 cut(s) 1793
DriI GACNNNNNGTC 2 cut(s) 1210, 1361
DseDI GACNNNNNNGTC 1 cut(s) 1793
EaeI YGGCCR 2 cut(s) 6, 78
Eam1104I CTCTTC 4 cut(s) 50, 435, 506, 1518
Eam1105I GACNNNNNGTC 2 cut(s) 1210, 1361
EarI CTCTTC 4 cut(s) 50, 435, 506, 1518
EciI GGCGGA 1 cut(s) 1661
Ecl136II GAGCTC 3 cut(s) 43, 985, 1528
Eco105I TACGTA 1 cut(s) 2116
Eco130I CCWWGG 4 cut(s) 9, 783, 1067, 1671
Eco147I AGGCCT 2 cut(s) 14, 1086
Eco24I GRGCYC 4 cut(s) 45, 927, 987, 1530
Eco32I GATATC 1 cut(s) 2095
Eco47I GGWCC 1 cut(s) 1212
Eco47III AGCGCT 1 cut(s) 470
Eco53kI GAGCTC 3 cut(s) 43, 985, 1528
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 1505
EcoICRI GAGCTC 3 cut(s) 43, 985, 1528
EcoNI CCTNNNNNAGG 1 cut(s) 1607
EcoRII CCWGG 2 cut(s) 807, 1737
EcoRV GATATC 1 cut(s) 2095
EcoT14I CCWWGG 4 cut(s) 9, 783, 1067, 1671
EcoT22I ATGCAT 1 cut(s) 1960
EcoT38I GRGCYC 4 cut(s) 45, 927, 987, 1530
ErhI CCWWGG 4 cut(s) 9, 783, 1067, 1671
FaqI GGGAC 2 cut(s) 1088, 1952
FauI CCCGC 1 cut(s) 478
FauNDI CATATG 1 cut(s) 616
FbaI TGATCA 2 cut(s) 1012, 1876
FblI GTMKAC 2 cut(s) 57, 1430
Fnu4HI GCNGC 5 cut(s) 129, 318, 467, 576, 832
FokI GGATG 8 cut(s) 80, 156, 504, 925, 1040, 1176, 1825, 1834
FriOI GRGCYC 4 cut(s) 45, 927, 987, 1530
Fsp4HI GCNGC 5 cut(s) 129, 318, 467, 576, 832
FspBI CTAG 1 cut(s) 945
GlaI GCGC 1 cut(s) 470
GluI GCNGC 5 cut(s) 129, 318, 467, 576, 832
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 2 cut(s) 1221, 1760
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 9 cut(s) 8, 14, 80, 346, 777, 807, 1086, 1323, 1633
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 471
Hin1I GRCGYC 1 cut(s) 1427
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HincII GTYRAC 2 cut(s) 58, 1431
HindII GTYRAC 2 cut(s) 58, 1431
HindIII AAGCTT 2 cut(s) 1901, 2063
HpaII CCGG 1 cut(s) 5
HphI GGTGA 8 cut(s) 100, 500, 575, 1391, 1405, 1459, 2023, 2030
Hpy166II GTNNAC 4 cut(s) 58, 1182, 1215, 1431
Hpy188III TCNNGA 9 cut(s) 49, 122, 749, 860, 896, 1253, 1353, 1880, 2057
Hpy8I GTNNAC 4 cut(s) 58, 1182, 1215, 1431
Hpy99I CGWCG 2 cut(s) 62, 1432
HpyAV CCTTC 5 cut(s) 16, 46, 868, 987, 1091
HpyCH4III ACNGT 3 cut(s) 1616, 1852, 2080
HpyCH4IV ACGT 6 cut(s) 105, 1008, 1410, 1427, 1445, 2115
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpySE526I ACGT 6 cut(s) 105, 1008, 1410, 1427, 1445, 2115
Hsp92I GRCGYC 1 cut(s) 1427
HspAI GCGC 1 cut(s) 469
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 1012, 1876
Kzo9I GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
LguI GCTCTTC 2 cut(s) 50, 1518
LmnI GCTCC 5 cut(s) 463, 922, 982, 1546, 1740
Lsp1109I GCAGC 4 cut(s) 115, 478, 587, 818
LweI GCATC 4 cut(s) 134, 262, 304, 1803
MaeI CTAG 1 cut(s) 945
MaeII ACGT 6 cut(s) 105, 1008, 1410, 1427, 1445, 2115
MalI GATC 6 cut(s) 935, 963, 1014, 1566, 1878, 1923
MbiI CCGCTC 1 cut(s) 73
MboI GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
MfeI CAATTG 1 cut(s) 907
MflI RGATCY 1 cut(s) 961
MhlI GDGCHC 5 cut(s) 45, 927, 987, 1530, 1692
MlyI GAGTC 7 cut(s) 730, 1405, 1507, 1631, 1658, 1705, 2047
MmeI TCCRAC 1 cut(s) 577
Mph1103I ATGCAT 1 cut(s) 1960
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 6 cut(s) 294, 425, 609, 648, 1146, 2073
MslI CAYNNNNRTG 3 cut(s) 729, 1194, 1376
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 809, 1739
MunI CAATTG 1 cut(s) 907
Mva1269I GAATGC 1 cut(s) 1312
MvaI CCWGG 2 cut(s) 809, 1739
MwoI GCNNNNNNNGC 1 cut(s) 153
NaeI GCCGGC 1 cut(s) 6
NdeI CATATG 1 cut(s) 616
NdeII GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
NgoMIV GCCGGC 1 cut(s) 4
NlaIV GGNNCC 4 cut(s) 201, 345, 924, 1662
NmuCI GTSAC 6 cut(s) 106, 488, 733, 1397, 2018, 2080
NsiI ATGCAT 1 cut(s) 1960
NspI RCATGY 5 cut(s) 384, 1153, 1199, 1340, 1796
NspV TTCGAA 1 cut(s) 2034
OliI CACNNNNGTG 1 cut(s) 729
PaeI GCATGC 1 cut(s) 1199
PaeR7I CTCGAG 1 cut(s) 1505
PceI AGGCCT 2 cut(s) 14, 1086
PciI ACATGT 2 cut(s) 1149, 1336
PciSI GCTCTTC 2 cut(s) 50, 1518
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 1312
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 5 cut(s) 1247, 1464, 1489, 1744, 2131
PflMI CCANNNNNTGG 1 cut(s) 1340
PfoI TCCNGGA 1 cut(s) 1737
PkrI GCNGC 5 cut(s) 130, 319, 468, 577, 833
PleI GAGTC 7 cut(s) 730, 1404, 1506, 1631, 1657, 1705, 2047
PpsI GAGTC 7 cut(s) 730, 1404, 1506, 1631, 1657, 1705, 2047
Ppu21I YACGTR 1 cut(s) 2116
PscI ACATGT 2 cut(s) 1149, 1336
Psp124BI GAGCTC 3 cut(s) 45, 987, 1530
Psp6I CCWGG 2 cut(s) 807, 1737
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 807, 1737
PspN4I GGNNCC 4 cut(s) 201, 345, 924, 1662
PspPI GGNCC 3 cut(s) 344, 1212, 1321
PspXI VCTCGAGB 1 cut(s) 1505
PstI CTGCAG 2 cut(s) 133, 577
PsuI RGATCY 1 cut(s) 961
RsaI GTAC 6 cut(s) 340, 1043, 1413, 1511, 1682, 2114
RsaNI GTAC 6 cut(s) 339, 1042, 1412, 1510, 1681, 2113
RseI CAYNNNNRTG 3 cut(s) 729, 1194, 1376
SacI GAGCTC 3 cut(s) 45, 987, 1530
SalI GTCGAC 2 cut(s) 56, 1429
SapI GCTCTTC 2 cut(s) 50, 1518
SaqAI TTAA 6 cut(s) 294, 425, 609, 648, 1146, 2073
SatI GCNGC 5 cut(s) 129, 318, 467, 576, 832
Sau3AI GATC 6 cut(s) 933, 961, 1012, 1564, 1876, 1921
Sau96I GGNCC 3 cut(s) 344, 1212, 1321
SchI GAGTC 7 cut(s) 730, 1405, 1507, 1631, 1658, 1705, 2047
ScrFI CCNGG 2 cut(s) 809, 1739
SduI GDGCHC 5 cut(s) 45, 927, 987, 1530, 1692
SfaNI GCATC 4 cut(s) 134, 262, 304, 1803
SfcI CTRYAG 2 cut(s) 129, 573
Sfr274I CTCGAG 1 cut(s) 1505
SfuI TTCGAA 1 cut(s) 2034
SinI GGWCC 1 cut(s) 1212
SlaI CTCGAG 1 cut(s) 1505
SmiMI CAYNNNNRTG 3 cut(s) 729, 1194, 1376
SmlI CTYRAG 5 cut(s) 38, 727, 1253, 1505, 1856
SmoI CTYRAG 5 cut(s) 38, 727, 1253, 1505, 1856
SnaBI TACGTA 1 cut(s) 2116
SpeI ACTAGT 1 cut(s) 944
SphI GCATGC 1 cut(s) 1199
SseBI AGGCCT 2 cut(s) 14, 1086
SsiI CCGC 6 cut(s) 73, 111, 317, 485, 1193, 1646
SspI AATATT 2 cut(s) 1261, 1349
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 3 cut(s) 45, 987, 1530
StuI AGGCCT 2 cut(s) 14, 1086
StyD4I CCNGG 2 cut(s) 807, 1737
StyI CCWWGG 4 cut(s) 9, 783, 1067, 1671
TaaI ACNGT 3 cut(s) 1616, 1852, 2080
TaiI ACGT 6 cut(s) 108, 1011, 1413, 1430, 1448, 2118
TaqI TCGA 7 cut(s) 57, 151, 1382, 1430, 1506, 1869, 2034
TatI WGTACW 2 cut(s) 1041, 1680
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 5 cut(s) 1247, 1464, 1489, 1744, 2131
Tru1I TTAA 6 cut(s) 294, 425, 609, 648, 1146, 2073
Tru9I TTAA 6 cut(s) 294, 425, 609, 648, 1146, 2073
TscAI CASTG 2 cut(s) 984, 2091
TseFI GTSAC 6 cut(s) 106, 488, 733, 1397, 2018, 2080
TseI GCWGC 4 cut(s) 128, 466, 575, 831
Tsp45I GTSAC 6 cut(s) 106, 488, 733, 1397, 2018, 2080
TspGWI ACGGA 2 cut(s) 75, 510
TspRI CASTG 2 cut(s) 984, 2091
Van91I CCANNNNNTGG 1 cut(s) 1340
VpaK11BI GGWCC 1 cut(s) 1212
XagI CCTNNNNNAGG 1 cut(s) 1607
XapI RAATTY 5 cut(s) 296, 353, 368, 603, 1274
XceI RCATGY 5 cut(s) 384, 1153, 1199, 1340, 1796
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XhoI CTCGAG 1 cut(s) 1505
XmiI GTMKAC 2 cut(s) 57, 1430
XspI CTAG 1 cut(s) 945
ZraI GACGTC 1 cut(s) 1428
Zsp2I ATGCAT 1 cut(s) 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.