FvH4_4g27080

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000872
Physical Location & Seq
Forward (+)
28219055 .. 28225144
6090 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27080.t1

Sequence Viewer

Length: 2385 bp
ATGCCGTCCAAGGAAGACGGCGCCTCCGATAAGGAGAAGATTCTCGAGCTTTTCTTGAAGGTCGGACTGGATGAGCGCACGGCCAAGAACACCATCGCTAACAACAAAGTCACGGCCAATCTAACCGCCGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGGAACGTCGGTAATCTTCTATACACGGTTGCTACGAAATTTCCAGCAAATGCCCTTGTGCATCGGCCGGTATTGACACAGTACATTGTGTCCTCAAAGATTAAAATTCCGGCTCAGTTAGACGCGGCTTTGTCTTTTTTCGCAAATACGGGCCCTGAAAACTTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTTGGGGTTGAAGTTTTAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAACAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAAAAATTGTAAAGGCATCGATTGATACAAAGCTGCGTGAATTACTTGGTGAAAGGACTGCAGAAGATGATGTAAAGGTTCCTAAAAAGAAGAAGGAGAAGCCGGCTAAAGTAGAGGAAAAAGCTATCGCTGTTTCTACCCCTGAACAACCAGCTGAAGAAATTCTCAATCCATATGCGATATTCCCTCAACCAGAGGAGAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCCATTCTGAGATGTTGCAATTCAAGAGAACTCCTTGAGAAACACTTGAGTGTGACTGGCGGGAAAGTTTTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTCATTGATTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTATGATGACACAAATCCAGAAGCTGAAAAGAAAGAATATATTGATCATATTCAGGAAATTGTCAGTTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGATTTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAGATGAACAGCCCTTGGAGGGATAGGCCAATTGAAGAGTCGCTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAAGCAACTCTAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTAATTGCGTATCGGATCAAATTTACTCCTCATCCGCATGCTGGAGACAAGTGGTGTATTTATCCAAGTTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATCACACATTCACTGTGCACTTTAGAATTTGAGACACGCCGTGCTTCATACTACTGGTTATTGCATTCTCTGGGCTTATATCAACCATATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGTAAGTTAAATAGATTAGTGACTGAAAATTGGGTCGATGGTTGGGATGATCCGCGTCTTATGACATTGGCTGGTTTACGACGTAGGGGTGTGACATCAACTGCCATTAATACATTTGTTAGAGGAATCGGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTGGATCCTGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGAATGTGAAGCAAGAAAGTGGCCTGATTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAATGGGTTGTACATTGAGCGCTCTGATTTTCGCCTTAAAGACTCGAAGGACTACTTCGGACTTGCTCCTGGAAAATCTGCACTGCTCAGATATGCATGTCCTATCAAGTGTGTGGATGCGAAGGATGTTGTTTTAGCAGAGGATAATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCAGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCCGAACCTTCTCCAGGGGTCGATCCACTCAAAGTGGAAGTTCGATTGTTTGACAGACTATTTAACTCAGAGAATCCTGCTGAGCTTGAGGACTGGCTTTCTGATCTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCACACTTGGGAAAGGTGTTGGTGTTGGCGACAAGTTTCAGTTTGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCCTGAGAAGCTTGTGTTCAATCGAACGGTCACGCTACGAGATAGCTACAAGAGTGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

795

Amino Acids

90.8

Weight (kDa)

6.17

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 13 - 170 3.5e-54 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 173 - 248 2.1e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 271 - 576 1.6e-121 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 579 - 682 1.5e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 692 - 770 9.4e-16 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AccI GTMKAC 1 cut(s) 2307
AccII CGCG 2 cut(s) 290, 1582
AciI CCGC 6 cut(s) 126, 290, 807, 1316, 1580, 1841
AclWI GGATC 6 cut(s) 1304, 1571, 1754, 1767, 2039, 2105
AcoI YGGCCR 3 cut(s) 81, 114, 230
AcsI RAATTY 6 cut(s) 203, 270, 341, 669, 1301, 1415
AcuI CTGAAG 2 cut(s) 684, 711
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 4 cut(s) 248, 1127, 1706, 1877
AfeI AGCGCT 2 cut(s) 446, 1886
AfiI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
AflIII ACRYGT 1 cut(s) 1272
AhdI GACNNNNNGTC 1 cut(s) 1502
AhlI ACTAGT 1 cut(s) 1028
AjnI CCWGG 3 cut(s) 891, 1933, 2101
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AleI CACNNNNGTG 1 cut(s) 795
Alw21I GWGCWC 3 cut(s) 1071, 1409, 1725
Alw26I GTCTC 4 cut(s) 484, 1320, 1415, 2009
Alw44I GTGCAC 1 cut(s) 1405
AlwI GGATC 6 cut(s) 1304, 1571, 1754, 1767, 2039, 2105
AlwNI CAGNNNCTG 2 cut(s) 152, 950
Ama87I CYCGRG 2 cut(s) 44, 1700
Aor51HI AGCGCT 2 cut(s) 446, 1886
AoxI GGCC 9 cut(s) 81, 114, 230, 316, 859, 889, 1163, 1207, 1826
ApaI GGGCCC 1 cut(s) 320
ApaLI GTGCAC 1 cut(s) 1405
ApeKI GCWGC 5 cut(s) 143, 442, 541, 915, 2077
ApoI RAATTY 6 cut(s) 203, 270, 341, 669, 1301, 1415
AseI ATTAAT 1 cut(s) 1635
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 4 cut(s) 23, 78, 447, 1887
AspS9I GGNCC 2 cut(s) 316, 317
AsuHPI GGTGA 3 cut(s) 476, 569, 1654
AvaI CYCGRG 2 cut(s) 44, 1700
BaeGI GKGCMC 2 cut(s) 320, 1409
BamHI GGATCC 1 cut(s) 1759
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 4 cut(s) 320, 1011, 1071, 1725
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 21, 2063
Bbv12I GWGCWC 3 cut(s) 1071, 1409, 1725
BbvI GCAGC 5 cut(s) 130, 454, 528, 902, 2064
BccI CCATC 5 cut(s) 101, 149, 737, 991, 1559
BceAI ACGGC 5 cut(s) 34, 96, 113, 129, 1413
BciT130I CCWGG 3 cut(s) 893, 1935, 2103
BclI TGATCA 2 cut(s) 970, 1096
BcoDI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BcuI ACTAGT 1 cut(s) 1028
BfaI CTAG 1 cut(s) 1029
BfmI CTRYAG 2 cut(s) 144, 567
BfoI RGCGCY 3 cut(s) 24, 448, 1888
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 6 cut(s) 144, 291, 443, 542, 916, 2078
BlpI GCTNAGC 1 cut(s) 2169
BlsI GCNGC 6 cut(s) 145, 292, 444, 543, 917, 2079
Bme1390I CCNGG 3 cut(s) 893, 1935, 2103
BmeRI GACNNNNNGTC 1 cut(s) 1502
BmeT110I CYCGRG 2 cut(s) 44, 1700
BmgT120I GGNCC 2 cut(s) 316, 317
BmiI GGNNCC 7 cut(s) 22, 318, 588, 748, 1008, 1761, 1857
BmrFI CCNGG 3 cut(s) 893, 1935, 2103
BmsI GCATC 4 cut(s) 149, 235, 533, 1972
BpiI GAAGAC 2 cut(s) 21, 2063
BplI GAGNNNNNCTC 2 cut(s) 1707, 1739
BpmI CTGGAG 2 cut(s) 1344, 2085
Bpu1102I GCTNAGC 1 cut(s) 2169
BpuEI CTTGAG 5 cut(s) 803, 814, 1403, 2045, 2195
Bsa29I ATCGAT 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 5 cut(s) 9, 497, 867, 1151, 2102
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
Bse118I RCCGGY 2 cut(s) 232, 610
Bse1I ACTGG 5 cut(s) 72, 808, 1448, 1790, 2186
Bse3DI GCAATG 2 cut(s) 879, 1363
BseBI CCWGG 3 cut(s) 893, 1935, 2103
BseCI ATCGAT 1 cut(s) 527
BseDI CCNNGG 5 cut(s) 9, 497, 867, 1151, 2102
BseGI GGATG 9 cut(s) 76, 164, 493, 1002, 1117, 1312, 1579, 1987, 1996
BseLI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
BseMI GCAATG 2 cut(s) 879, 1363
BseMII CTCAG 9 cut(s) 293, 746, 914, 1682, 1827, 1966, 2160, 2169, 2316
BseNI ACTGG 5 cut(s) 72, 808, 1448, 1790, 2186
BseRI GAGGAG 3 cut(s) 719, 1299, 1728
BseSI GKGCMC 2 cut(s) 320, 1409
BseX3I CGGCCG 1 cut(s) 230
BseXI GCAGC 5 cut(s) 130, 454, 528, 902, 2064
BsgI GTGCAG 1 cut(s) 1929
Bsh1236I CGCG 2 cut(s) 290, 1582
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 9 cut(s) 83, 116, 232, 318, 861, 891, 1165, 1209, 1828
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 527
BsiEI CGRYCG 1 cut(s) 233
BsiHKAI GWGCWC 3 cut(s) 1071, 1409, 1725
BsiHKCI CYCGRG 2 cut(s) 44, 1700
BsiSI CCGG 3 cut(s) 233, 275, 611
BslI CCNNNNNNNGG 7 cut(s) 868, 1156, 1322, 1804, 2102, 2246, 2247
BsmAI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BsmI GAATGC 1 cut(s) 1453
BsnI GGCC 9 cut(s) 83, 116, 232, 318, 861, 891, 1165, 1209, 1828
BsoBI CYCGRG 2 cut(s) 44, 1700
Bsp120I GGGCCC 1 cut(s) 316
Bsp1286I GDGCHC 5 cut(s) 320, 1011, 1071, 1409, 1725
Bsp1407I TGTACA 1 cut(s) 1875
Bsp1720I GCTNAGC 1 cut(s) 2169
BspACI CCGC 6 cut(s) 126, 290, 807, 1316, 1580, 1841
BspANI GGCC 9 cut(s) 83, 116, 232, 318, 861, 891, 1165, 1209, 1828
BspCNI CTCAG 9 cut(s) 292, 747, 915, 1683, 1828, 1965, 2161, 2168, 2317
BspDI ATCGAT 1 cut(s) 527
BspFNI CGCG 2 cut(s) 290, 1582
BspLI GGNNCC 7 cut(s) 22, 318, 588, 748, 1008, 1761, 1857
BspMAI CTGCAG 2 cut(s) 148, 571
BspPI GGATC 6 cut(s) 1304, 1571, 1754, 1767, 2039, 2105
BspQI GCTCTTC 1 cut(s) 1713
BspT107I GGYRCC 1 cut(s) 20
BsrDI GCAATG 2 cut(s) 879, 1363
BsrFI RCCGGY 2 cut(s) 232, 610
BsrGI TGTACA 1 cut(s) 1875
BsrI ACTGG 5 cut(s) 72, 808, 1448, 1790, 2186
BssAI RCCGGY 2 cut(s) 232, 610
BssECI CCNNGG 5 cut(s) 9, 497, 867, 1151, 2102
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 137
BssT1I CCWWGG 3 cut(s) 9, 867, 1151
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 3 cut(s) 893, 1935, 2103
Bst4CI ACNGT 6 cut(s) 193, 246, 1404, 1516, 2020, 2350
Bst6I CTCTTC 4 cut(s) 411, 482, 1167, 1713
BstACI GRCGYC 1 cut(s) 21
BstAUI TGTACA 1 cut(s) 1875
BstC8I GCNNGC 2 cut(s) 612, 1320
BstDSI CCRYGG 1 cut(s) 497
BstENI CCTNNNNNAGG 2 cut(s) 1802, 2100
BstF5I GGATG 9 cut(s) 76, 164, 493, 1002, 1117, 1312, 1579, 1987, 1996
BstFNI CGCG 2 cut(s) 290, 1582
BstH2I RGCGCY 3 cut(s) 24, 448, 1888
BstHHI GCGC 4 cut(s) 23, 78, 447, 1887
BstMAI GTCTC 4 cut(s) 484, 1320, 1415, 2009
BstMCI CGRYCG 1 cut(s) 233
BstNI CCWGG 3 cut(s) 893, 1935, 2103
BstNSI RCATGY 4 cut(s) 357, 1276, 1322, 1965
BstSCI CCNGG 3 cut(s) 891, 1933, 2101
BstSFI CTRYAG 2 cut(s) 144, 567
BstSLI GKGCMC 2 cut(s) 320, 1409
BstUI CGCG 2 cut(s) 290, 1582
BstV1I GCAGC 5 cut(s) 130, 454, 528, 902, 2064
BstV2I GAAGAC 2 cut(s) 21, 2063
BstX2I RGATCY 2 cut(s) 1045, 1759
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 2 cut(s) 1045, 1759
BstZI CGGCCG 1 cut(s) 230
Bsu15I ATCGAT 1 cut(s) 527
BsuRI GGCC 9 cut(s) 83, 116, 232, 318, 861, 891, 1165, 1209, 1828
BsuTUI ATCGAT 1 cut(s) 527
BtgI CCRYGG 1 cut(s) 497
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 9 cut(s) 76, 164, 493, 1002, 1117, 1312, 1579, 1987, 1996
BtsI GCAGTG 2 cut(s) 1068, 1946
BtsIMutI CAGTG 4 cut(s) 1068, 1400, 1512, 1946
Cac8I GCNNGC 2 cut(s) 612, 1320
CaiI CAGNNNCTG 2 cut(s) 152, 950
CfoI GCGC 4 cut(s) 23, 78, 447, 1887
Cfr10I RCCGGY 2 cut(s) 232, 610
Cfr13I GGNCC 2 cut(s) 316, 317
ClaI ATCGAT 1 cut(s) 527
CseI GACGC 2 cut(s) 296, 1571
Csp6I GTAC 4 cut(s) 247, 1126, 1705, 1876
CviAII CATG 5 cut(s) 354, 863, 1273, 1319, 1962
CviQI GTAC 4 cut(s) 247, 1126, 1705, 1876
DinI GGCGCC 1 cut(s) 22
DraIII CACNNNGTG 1 cut(s) 1430
DriI GACNNNNNGTC 1 cut(s) 1502
EaeI YGGCCR 3 cut(s) 81, 114, 230
EagI CGGCCG 1 cut(s) 230
Eam1104I CTCTTC 4 cut(s) 411, 482, 1167, 1713
Eam1105I GACNNNNNGTC 1 cut(s) 1502
EarI CTCTTC 4 cut(s) 411, 482, 1167, 1713
EciI GGCGGA 1 cut(s) 1856
Ecl136II GAGCTC 2 cut(s) 1069, 1723
EclXI CGGCCG 1 cut(s) 230
Eco130I CCWWGG 3 cut(s) 9, 867, 1151
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 4 cut(s) 320, 1011, 1071, 1725
Eco32I GATATC 1 cut(s) 848
Eco47III AGCGCT 2 cut(s) 446, 1886
Eco52I CGGCCG 1 cut(s) 230
Eco53kI GAGCTC 2 cut(s) 1069, 1723
Eco57I CTGAAG 2 cut(s) 684, 711
Eco88I CYCGRG 2 cut(s) 44, 1700
EcoICRI GAGCTC 2 cut(s) 1069, 1723
EcoNI CCTNNNNNAGG 2 cut(s) 1802, 2100
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 891, 1933, 2101
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 3 cut(s) 9, 867, 1151
EcoT22I ATGCAT 2 cut(s) 1963, 2230
EcoT38I GRGCYC 4 cut(s) 320, 1011, 1071, 1725
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 3 cut(s) 9, 867, 1151
FaeI CATG 5 cut(s) 357, 866, 1276, 1322, 1965
FalI AAGNNNNNCTT 2 cut(s) 1904, 1936
FatI CATG 5 cut(s) 353, 862, 1272, 1318, 1961
FauI CCCGC 1 cut(s) 800
FauNDI CATATG 2 cut(s) 682, 1477
FbaI TGATCA 2 cut(s) 970, 1096
FblI GTMKAC 1 cut(s) 2307
Fnu4HI GCNGC 6 cut(s) 144, 291, 443, 542, 916, 2078
FokI GGATG 9 cut(s) 83, 171, 480, 1009, 1124, 1299, 1586, 1994, 2003
FriOI GRGCYC 4 cut(s) 320, 1011, 1071, 1725
Fsp4HI GCNGC 6 cut(s) 144, 291, 443, 542, 916, 2078
FspBI CTAG 1 cut(s) 1029
GlaI GCGC 4 cut(s) 22, 77, 446, 1886
GluI GCNGC 6 cut(s) 144, 291, 443, 542, 916, 2078
GsuI CTGGAG 2 cut(s) 1344, 2085
HaeII RGCGCY 3 cut(s) 24, 448, 1888
HaeIII GGCC 9 cut(s) 83, 116, 232, 318, 861, 891, 1165, 1209, 1828
HapII CCGG 3 cut(s) 233, 275, 611
HgaI GACGC 2 cut(s) 296, 1571
HhaI GCGC 4 cut(s) 23, 78, 447, 1887
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 5 cut(s) 357, 866, 1276, 1322, 1965
Hin6I GCGC 4 cut(s) 21, 76, 445, 1885
HinP1I GCGC 4 cut(s) 21, 76, 445, 1885
HincII GTYRAC 1 cut(s) 2308
HindII GTYRAC 1 cut(s) 2308
HindIII AAGCTT 1 cut(s) 2330
HpaII CCGG 3 cut(s) 233, 275, 611
HphI GGTGA 3 cut(s) 476, 569, 1654
Hpy166II GTNNAC 3 cut(s) 1407, 1604, 2308
Hpy8I GTNNAC 3 cut(s) 1407, 1604, 2308
Hpy99I CGWCG 2 cut(s) 176, 1611
HpyAV CCTTC 7 cut(s) 52, 595, 1071, 1214, 1906, 1981, 2106
HpyCH4III ACNGT 6 cut(s) 193, 246, 1404, 1516, 2020, 2350
HpyCH4IV ACGT 3 cut(s) 171, 1092, 1609
HpySE526I ACGT 3 cut(s) 171, 1092, 1609
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 5 cut(s) 357, 866, 1276, 1322, 1965
HspAI GCGC 4 cut(s) 21, 76, 445, 1885
KasI GGCGCC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 610
KroNI GCCGGC 1 cut(s) 612
Ksp22I TGATCA 2 cut(s) 970, 1096
LguI GCTCTTC 1 cut(s) 1713
LmnI GCTCC 5 cut(s) 439, 1006, 1066, 1741, 1936
Lsp1109I GCAGC 5 cut(s) 130, 454, 528, 902, 2064
LweI GCATC 4 cut(s) 149, 235, 533, 1972
MaeI CTAG 1 cut(s) 1029
MaeII ACGT 3 cut(s) 171, 1092, 1609
MfeI CAATTG 1 cut(s) 1167
MflI RGATCY 2 cut(s) 1045, 1759
MhlI GDGCHC 5 cut(s) 320, 1011, 1071, 1409, 1725
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 5 cut(s) 1184, 1702, 1853, 1901, 2314
MmeI TCCRAC 3 cut(s) 43, 146, 974
Mph1103I ATGCAT 2 cut(s) 1963, 2230
MroNI GCCGGC 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 9 cut(s) 267, 402, 714, 818, 1269, 1535, 1635, 1902, 2151
MslI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 3 cut(s) 233, 275, 611
MspR9I CCNGG 3 cut(s) 893, 1935, 2103
MunI CAATTG 1 cut(s) 1167
Mva1269I GAATGC 1 cut(s) 1453
MvaI CCWGG 3 cut(s) 893, 1935, 2103
MvnI CGCG 2 cut(s) 290, 1582
NaeI GCCGGC 1 cut(s) 612
NarI GGCGCC 1 cut(s) 21
NdeI CATATG 2 cut(s) 682, 1477
NgoMIV GCCGGC 1 cut(s) 610
NlaIII CATG 5 cut(s) 357, 866, 1276, 1322, 1965
NlaIV GGNNCC 7 cut(s) 22, 318, 588, 748, 1008, 1761, 1857
NmuCI GTSAC 7 cut(s) 109, 464, 799, 1082, 1546, 1618, 2350
NsiI ATGCAT 2 cut(s) 1963, 2230
NspI RCATGY 4 cut(s) 357, 1276, 1322, 1965
OliI CACNNNNGTG 1 cut(s) 795
PaeI GCATGC 1 cut(s) 1322
PaeR7I CTCGAG 2 cut(s) 44, 1700
PceI AGGCCT 1 cut(s) 1209
PciI ACATGT 1 cut(s) 1272
PciSI GCTCTTC 1 cut(s) 1713
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 197
PctI GAATGC 1 cut(s) 1453
PdiI GCCGGC 1 cut(s) 612
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 8 cut(s) 40, 1376, 1653, 1659, 1684, 1832, 2161, 2315
PfoI TCCNGGA 1 cut(s) 1933
PkrI GCNGC 6 cut(s) 145, 292, 444, 543, 917, 2079
PleI GAGTC 5 cut(s) 1183, 1701, 1852, 1901, 2314
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 5 cut(s) 1183, 1701, 1852, 1901, 2314
PscI ACATGT 1 cut(s) 1272
PshBI ATTAAT 1 cut(s) 1635
Psp124BI GAGCTC 2 cut(s) 1071, 1725
Psp6I CCWGG 3 cut(s) 891, 1933, 2101
PspGI CCWGG 3 cut(s) 891, 1933, 2101
PspN4I GGNNCC 7 cut(s) 22, 318, 588, 748, 1008, 1761, 1857
PspOMI GGGCCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 316, 317
PspXI VCTCGAGB 1 cut(s) 1700
PstI CTGCAG 2 cut(s) 148, 571
PstNI CAGNNNCTG 2 cut(s) 152, 950
PsuI RGATCY 2 cut(s) 1045, 1759
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 4 cut(s) 248, 1127, 1706, 1877
RsaNI GTAC 4 cut(s) 247, 1126, 1705, 1876
RseI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
SacI GAGCTC 2 cut(s) 1071, 1725
SalI GTCGAC 1 cut(s) 2306
SapI GCTCTTC 1 cut(s) 1713
SaqAI TTAA 9 cut(s) 267, 402, 714, 818, 1269, 1535, 1635, 1902, 2151
SatI GCNGC 6 cut(s) 144, 291, 443, 542, 916, 2078
Sau96I GGNCC 2 cut(s) 316, 317
SchI GAGTC 5 cut(s) 1184, 1702, 1853, 1901, 2314
ScrFI CCNGG 3 cut(s) 893, 1935, 2103
SduI GDGCHC 5 cut(s) 320, 1011, 1071, 1409, 1725
SfaNI GCATC 4 cut(s) 149, 235, 533, 1972
SfcI CTRYAG 2 cut(s) 144, 567
SfoI GGCGCC 1 cut(s) 22
Sfr274I CTCGAG 2 cut(s) 44, 1700
SlaI CTCGAG 2 cut(s) 44, 1700
SmiMI CAYNNNNRTG 3 cut(s) 795, 1317, 1517
SmlI CTYRAG 7 cut(s) 44, 782, 793, 1382, 1700, 2024, 2174
SmoI CTYRAG 7 cut(s) 44, 782, 793, 1382, 1700, 2024, 2174
SpeI ACTAGT 1 cut(s) 1028
SphI GCATGC 1 cut(s) 1322
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 6 cut(s) 126, 290, 807, 1316, 1580, 1841
SspDI GGCGCC 1 cut(s) 20
SspI AATATT 1 cut(s) 1490
SspMI CTAG 1 cut(s) 1029
SstI GAGCTC 2 cut(s) 1071, 1725
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 3 cut(s) 891, 1933, 2101
StyI CCWWGG 3 cut(s) 9, 867, 1151
TaaI ACNGT 6 cut(s) 193, 246, 1404, 1516, 2020, 2350
TaiI ACGT 3 cut(s) 174, 1095, 1612
TatI WGTACW 3 cut(s) 246, 1125, 1875
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 8 cut(s) 40, 1376, 1653, 1659, 1684, 1832, 2161, 2315
Tru1I TTAA 9 cut(s) 267, 402, 714, 818, 1269, 1535, 1635, 1902, 2151
Tru9I TTAA 9 cut(s) 267, 402, 714, 818, 1269, 1535, 1635, 1902, 2151
TscAI CASTG 4 cut(s) 1068, 1407, 1519, 1953
TseFI GTSAC 7 cut(s) 109, 464, 799, 1082, 1546, 1618, 2350
TseI GCWGC 5 cut(s) 143, 442, 541, 915, 2077
Tsp45I GTSAC 7 cut(s) 109, 464, 799, 1082, 1546, 1618, 2350
TspDTI ATGAA 6 cut(s) 710, 868, 1157, 1256, 1425, 1676
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 4 cut(s) 1068, 1407, 1519, 1953
VneI GTGCAC 1 cut(s) 1405
VspI ATTAAT 1 cut(s) 1635
XagI CCTNNNNNAGG 2 cut(s) 1802, 2100
XapI RAATTY 6 cut(s) 203, 270, 341, 669, 1301, 1415
XceI RCATGY 4 cut(s) 357, 1276, 1322, 1965
XhoI CTCGAG 2 cut(s) 44, 1700
XmiI GTMKAC 1 cut(s) 2307
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 1 cut(s) 1029
Zsp2I ATGCAT 2 cut(s) 1963, 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.