Rroxscaffold_4G00325420

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000872
Physical Location & Seq
Reverse (-)
57154510 .. 57156451
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325420.1

Sequence Viewer

Length: 456 bp
ATGCTCATTGCATTGTGGATTCTCTTGAGAATATCATCCATGCTGTGCACTTCGGAATTTGAGACACGGCGTGCTTCATACTACCGGTTATTGCATTCTCTAGGCCTATATCAACCACATGTTTGGGAATATTCAAGACCGGATGTCGGTAACACTATTATGTCGAAGCCTTTCGATCTGAATGAAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCCCACTTGAGAAAGGTGTTGGTGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCTTGAGAAGCTTATATTTAATCGTATGGTCACACTGCGAGATAGCTATAAGAGTGGGAAGTACGTCATCTTTGGAATCATCATAATAGAGAGGAATATATATGCTTTGTTACACCTAGACCATGGTTCACATATGCAAGACTTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

151

Amino Acids

17.51

Weight (kDa)

8.65

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 8 - 56 1.8e-07 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C2 PF20974 58 - 98 7.2e-07 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 56
AdeI CACNNNGTG 1 cut(s) 71
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 118
AgeI ACCGGT 1 cut(s) 84
AgsI TTSAA 1 cut(s) 135
AhdI GACNNNNNGTC 1 cut(s) 143
AluBI AGCT 2 cut(s) 316, 351
AluI AGCT 2 cut(s) 316, 351
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 56
Alw44I GTGCAC 1 cut(s) 46
AoxI GGCC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 56
AsiGI ACCGGT 1 cut(s) 84
Asp700I GAANNNNTTC 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 2 cut(s) 101, 422
BmeRI GACNNNNNGTC 1 cut(s) 143
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 46, 251, 329
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 2 cut(s) 84, 139
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 2 cut(s) 35, 148
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 6
BseSI GKGCMC 1 cut(s) 50
BshFI GGCC 1 cut(s) 105
BshTI ACCGGT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 85, 140
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 1 cut(s) 175
Bsp19I CCATGG 1 cut(s) 427
BspANI GGCC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 2 cut(s) 35, 148
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstNSI RCATGY 1 cut(s) 122
BstSLI GKGCMC 1 cut(s) 50
BstXI CCANNNNNNTGG 1 cut(s) 123
BsuRI GGCC 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 2 cut(s) 35, 148
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 84
Csp6I GTAC 1 cut(s) 367
CspAI ACCGGT 1 cut(s) 84
CviAII CATG 3 cut(s) 40, 119, 428
CviJI RGCY 5 cut(s) 105, 169, 279, 316, 351
CviKI_1 RGCY 5 cut(s) 105, 169, 279, 316, 351
CviQI GTAC 1 cut(s) 367
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 71
DriI GACNNNNNGTC 1 cut(s) 143
Eam1105I GACNNNNNGTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 427
Eco147I AGGCCT 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 427
EcoT22I ATGCAT 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 3 cut(s) 43, 122, 431
FatI CATG 3 cut(s) 39, 118, 427
FauNDI CATATG 1 cut(s) 438
FblI GTMKAC 1 cut(s) 291
FokI GGATG 2 cut(s) 22, 155
FspBI CTAG 2 cut(s) 101, 422
HaeIII GGCC 1 cut(s) 105
HapII CCGG 2 cut(s) 85, 140
Hin1II CATG 3 cut(s) 43, 122, 431
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 4 cut(s) 19, 299, 304, 381
HpaII CCGG 2 cut(s) 85, 140
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 3 cut(s) 48, 292, 434
Hpy188I TCNGA 2 cut(s) 55, 180
Hpy188III TCNNGA 3 cut(s) 25, 135, 308
Hpy8I GTNNAC 3 cut(s) 48, 292, 434
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 11, 48, 94, 212, 442, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 3 cut(s) 43, 122, 431
Kzo9I GATC 1 cut(s) 175
LpnPI CCDG 2 cut(s) 98, 153
MaeI CTAG 2 cut(s) 101, 422
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 4 cut(s) 149, 251, 334, 414
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 1 cut(s) 56
MlyI GAGTC 1 cut(s) 298
MnlI CCTC 1 cut(s) 390
Mph1103I ATGCAT 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 85, 140
Mva1269I GAATGC 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 448
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 438
NdeII GATC 1 cut(s) 175
NlaIII CATG 3 cut(s) 43, 122, 431
NmuCI GTSAC 2 cut(s) 251, 334
NsiI ATGCAT 1 cut(s) 214
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 105
PciI ACATGT 1 cut(s) 118
PctI GAATGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 19, 299, 381
PinAI ACCGGT 1 cut(s) 84
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PscI ACATGT 1 cut(s) 118
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 158
SalI GTCGAC 1 cut(s) 290
SaqAI TTAA 1 cut(s) 324
Sau3AI GATC 1 cut(s) 175
SchI GAGTC 1 cut(s) 298
SduI GDGCHC 1 cut(s) 50
SetI ASST 5 cut(s) 241, 318, 353, 372, 423
SfuI TTCGAA 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 3 cut(s) 25, 230, 308
SmoI CTYRAG 3 cut(s) 25, 230, 308
Sse9I AATT 1 cut(s) 56
SseBI AGGCCT 1 cut(s) 105
SspI AATATT 1 cut(s) 131
SspMI CTAG 2 cut(s) 101, 422
StuI AGGCCT 1 cut(s) 105
StyI CCWWGG 1 cut(s) 427
TaiI ACGT 1 cut(s) 372
TaqI TCGA 6 cut(s) 164, 174, 191, 267, 291, 302
TasI AATT 1 cut(s) 56
TfiI GAWTC 3 cut(s) 19, 299, 381
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 2 cut(s) 251, 334
Tsp45I GTSAC 2 cut(s) 251, 334
TspDTI ATGAA 2 cut(s) 66, 198
TspRI CASTG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 46
XapI RAATTY 1 cut(s) 56
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 101, 422
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.