RLG00000006639

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000872
Physical Location & Seq
Reverse (-)
9272491 .. 9282519
10029 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006639

Sequence Viewer

Length: 3738 bp
ATGGAGCCGTGGAAGAACACCATGAAGAAGAAGCGGAGGCCCACCCGGAGGCCAGTCCGGCTCCGCTGCTCCGTCAAGAATTACGACTGGGGCATTCTCGGCCACAATTCCAAGGTCGCCAGGCTCTTCGCTCTCAATTCCGGGTCGGACATCGACCCGGCCAAGCCCTACGCCGAGTTCTGGATCGGGACTCATGAATCCGGGCCGTCCTACGCCGAGTCCGGGTCGGAGCAGCCCCTCAGCCTCAAGTCCTGGATCGCCGAGGATCCGAGTGTTCTCGGCGAGAAGGTTCTGGACAAGTGGGGCGCCGATCTTCCGTTCTTGTGCAAGGTACTTTCGGTTCAGAAGGCTCTGTCAATACAGGCTCATCCGGATAAAGAGATGGCTAGAGTGTTGCACAAGTTGCACCCCAGTGTTTACAAGGATGATAATCACAAGCCTGAAGTGGCATTGGCGCTGACGGAGTTCGAGGCTCTTTGCGGGTTTATTAGTCTCAAGGAGCTAAAAGACATGCTTCATACTGTTCCTGAGATTGCCGAATTGGTTGGGGTGGCAGATGCAGAGCAAATTCTGCTTGTTGGTGAGCAACATGAGAATGGGAAACTAAACACAGATTTAGAGTCCATGTTCAGTCGACTCATGTTGTCAAGCAAAGAGACAATTTCTAAGATGATATCCAAACTAAAAAGGCGTCTGAACCTTGAGAAAAAGAGAAGGCAATTGACAGTTAAAGAACAACTGGTTCTCAGATTAGAGAATCAATATCCAGCCGATGTGGGTGCTATAGCTGCATTTTTCCTGAACTATGTGAAGCTGAAACGTGGTGAAGCATTATATTGTGGGCCAAATGAACCCCATGCATATATTTCTGGTGAATGCGTTGAATGTATGGCGACCTCTGACAATGTTGTTCGAGCTGGCCTTACCTCAAAACAAATTGATGTTCAGACACTCCTTTCTATGCTCAAATACAGACAGGGAGCTCCTGAAATTATGCAAGGAGTTTCTATGAACGCATACACAACAAGATTTGTTCCACCTTTTGAAGAATTTGAAGTCGACTGCTGTGATCTTCCTCAGTCTGCTTCGGTAGTTTTTCCCTCCGTCATAGGACCATCCCTTTTCCTAGTCACTGCAGGGAAAGGCAGGTTTGATGCAGGCTTGGCTGAGGATGATGTAGTTACAGAAGATGATGATATAGTTGAGGAGGGCGAGGTTCTATTTGTGCCTGCCCACACCAAGATTAGCATTACTGCTAGATCAACAGAACTACAATTGGTTTATCTTCACAGCAGCTCACCTAAGCTAGCTTCACAGACCGACGCACTTGGTTGTCCTTCGCCGCCGTTCGGGATGCCGACCAAGGACGACGGCGCCGCCGAGAAGGAGAAGAACCTTGAGCTCTTCCTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACCATCGCCAACAACAAGGTCACGGCGAATCTAATGGCCGTCATTCACGAGGCTGGAGTTACTGATGGATGCAGTCGAAGCGTTGGTAATCTTCTATACACGGTTGCTACAAAGTTTCCGGCAAATGCCCTTGTGCATCGCCCCGTGTTAACACAGTACATTGTGTCTTCAAAGATTAAAATCCCGGCTCAGTTAGATGCGGCTTTGTCATATTTTGTGAATACGGGGCCAGAGAACTTCAAAACAACTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAACAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCCTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAAATTGCGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTCTACTCCTGAACAACCAGCTGAAGAAATTCTAAATCCATATGCAATATTCCCTCAGCCAGACGAAAACTTCAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGTGTGACTGGGGGCAGAGTTTTAACTCGCTTTCCTCCAGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTATGTTGATCATCAGACAGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCACTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAAGCAACTATAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTTATTGCGTATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAAGTGGTGTATCTATCCGAGTTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATCACCCATTCACTGTGCACTTTAGAATTTGAGACACGCCGTGCTTCATACTACTGGCTATTGCATGCTTTGGGCCTTTATCAACCATATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCAAAGCGTAAGTTAAATCGACTGGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTGGCTGGTTTACGACGTAGGGGTGTGACGTCGACTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTTGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCACGCGCAATGGTTGTGCTTGATCCTGTAAAGGTTGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGATTGCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGATTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGGTTGATCCACTCAAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCCCACTTGGGAAAGGTGTTGGTGTTGGCGACAAGTTTCAGTTTGAAAGACTTGGGTATTTTGTGGTCGACAAAGATACGACTCCGGAGAAGCTTGTGTTTAATCGTACGGTTACGCTGCGAGATAGCTACAAGAGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

1246

Amino Acids

140.87

Weight (kDa)

6.27

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 20 - 162 1.5e-44 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 190 - 267 4.8e-10 Phosphomannose isomerase type I, helical insertion domain
tRNA_synt_1c_R1 PF04558 464 - 621 7.6e-55 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 624 - 699 3.6e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 723 - 1027 7.5e-122 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 1030 - 1133 6e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 1143 - 1221 4.1e-15 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2978
Acc36I ACCTGC 1 cut(s) 1137
AccB1I GGYRCC 2 cut(s) 305, 1373
AccB7I CCANNNNNTGG 1 cut(s) 2675
AccBSI CCGCTC 1 cut(s) 1429
AccI GTMKAC 4 cut(s) 634, 1059, 2978, 3660
AccII CGCG 1 cut(s) 3096
AccIII TCCGGA 4 cut(s) 370, 2296, 3400, 3676
AciI CCGC 8 cut(s) 34, 64, 480, 1343, 1377, 1429, 1643, 3194
AclWI GGATC 8 cut(s) 191, 260, 263, 273, 2924, 3107, 3392, 3458
AcoI YGGCCR 4 cut(s) 100, 159, 1434, 1479
AcsI RAATTY 5 cut(s) 567, 1049, 1694, 2022, 2768
AcuI CTGAAG 4 cut(s) 462, 1430, 2037, 2064
AcyI GRCGYC 4 cut(s) 306, 691, 1374, 2975
AdeI CACNNNGTG 1 cut(s) 2783
AfaI GTAC 6 cut(s) 333, 1601, 2480, 3059, 3230, 3700
AfeI AGCGCT 2 cut(s) 1799, 3239
AflIII ACRYGT 1 cut(s) 2625
AhdI GACNNNNNGTC 1 cut(s) 2855
AhlI ACTAGT 1 cut(s) 2381
AjnI CCWGG 6 cut(s) 119, 251, 1850, 2244, 3286, 3454
AleI CACNNNNGTG 2 cut(s) 411, 2148
Alw21I GWGCWC 5 cut(s) 985, 1404, 2424, 2762, 3078
Alw26I GTCTC 6 cut(s) 497, 650, 1837, 2673, 2768, 3362
Alw44I GTGCAC 1 cut(s) 2758
AlwI GGATC 8 cut(s) 191, 260, 263, 273, 2924, 3107, 3392, 3458
Aor13HI TCCGGA 4 cut(s) 370, 2296, 3400, 3676
Aor51HI AGCGCT 2 cut(s) 1799, 3239
ApaLI GTGCAC 1 cut(s) 2758
ApeKI GCWGC 9 cut(s) 66, 232, 788, 1293, 1795, 1922, 2268, 3430, 3709
ApoI RAATTY 5 cut(s) 567, 1049, 1694, 2022, 2768
ArsI GACNNNNNNTTYG 2 cut(s) 3465, 3497
AseI ATTAAT 1 cut(s) 2988
Asp700I GAANNNNTTC 2 cut(s) 1938, 2022
AspLEI GCGC 6 cut(s) 308, 457, 1376, 1800, 3098, 3240
AspS9I GGNCC 7 cut(s) 39, 203, 842, 1112, 1670, 2815, 3180
AsuC2I CCSGG 6 cut(s) 46, 142, 158, 202, 223, 1628
AsuHPI GGTGA 9 cut(s) 593, 836, 884, 1290, 1829, 1922, 2737, 2911, 3007
AsuNHI GCTAGC 1 cut(s) 1306
AvaII GGWCC 1 cut(s) 1112
BaeGI GKGCMC 1 cut(s) 2762
BaeI ACNNNNGTAYC 2 cut(s) 3301, 3334
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 305, 1373
BanII GRGCYC 5 cut(s) 985, 1404, 2364, 2424, 3078
BauI CACGAG 1 cut(s) 1490
BbsI GAAGAC 2 cut(s) 1602, 3416
Bbv12I GWGCWC 5 cut(s) 985, 1404, 2424, 2762, 3078
BbvCI CCTCAGC 4 cut(s) 239, 1167, 2049, 3357
BbvI GCAGC 9 cut(s) 53, 244, 775, 1305, 1807, 1934, 2255, 3417, 3696
BccI CCATC 7 cut(s) 376, 1123, 1454, 1502, 2090, 2344, 2912
BceAI ACGGC 7 cut(s) 190, 1330, 1387, 1449, 1466, 1482, 2766
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 6 cut(s) 121, 253, 1852, 2246, 3288, 3456
BclI TGATCA 2 cut(s) 2323, 2449
BcnI CCSGG 6 cut(s) 46, 142, 158, 202, 223, 1628
BcoDI GTCTC 6 cut(s) 497, 650, 1837, 2673, 2768, 3362
BcuI ACTAGT 1 cut(s) 2381
BfaI CTAG 5 cut(s) 387, 1127, 1257, 1307, 2382
BfmI CTRYAG 3 cut(s) 783, 1134, 1920
BfoI RGCGCY 5 cut(s) 309, 458, 1377, 1801, 3241
BfuAI ACCTGC 1 cut(s) 1137
BglI GCCNNNNNGGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 2398
BlpI GCTNAGC 1 cut(s) 3522
Bme18I GGWCC 1 cut(s) 1112
BmeRI GACNNNNNGTC 1 cut(s) 2855
BmgT120I GGNCC 7 cut(s) 39, 203, 842, 1112, 1670, 2815, 3180
BmiI GGNNCC 9 cut(s) 6, 62, 267, 307, 1375, 1671, 1941, 2361, 3210
BmrI ACTGGG 3 cut(s) 97, 405, 2166
BmsI GCATC 8 cut(s) 547, 1144, 1344, 1502, 1588, 1630, 1886, 3325
BmtI GCTAGC 1 cut(s) 1310
BmuI ACTGGG 3 cut(s) 97, 405, 2166
BpiI GAAGAC 2 cut(s) 1602, 3416
BplI GAGNNNNNCTC 2 cut(s) 3060, 3092
BpmI CTGGAG 3 cut(s) 1518, 2169, 2697
Bpu10I CCTNAGC 5 cut(s) 239, 1167, 1302, 2049, 3357
Bpu1102I GCTNAGC 1 cut(s) 3522
BpuEI CTTGAG 8 cut(s) 230, 479, 722, 1418, 2167, 2756, 3074, 3398
BpuMI CCSGG 6 cut(s) 46, 142, 158, 202, 223, 1628
BsaBI GATNNNNATC 2 cut(s) 429, 1622
BsaHI GRCGYC 4 cut(s) 306, 691, 1374, 2975
BsaWI WCCGGW 4 cut(s) 370, 2296, 3400, 3676
Bse3DI GCAATG 3 cut(s) 2232, 2716, 3105
Bse8I GATNNNNATC 2 cut(s) 429, 1622
BseAI TCCGGA 4 cut(s) 370, 2296, 3400, 3676
BseBI CCWGG 6 cut(s) 121, 253, 1852, 2246, 3288, 3456
BseJI GATNNNNATC 2 cut(s) 429, 1622
BseMI GCAATG 3 cut(s) 2232, 2716, 3105
BseRI GAGGAG 2 cut(s) 1220, 2652
BseSI GKGCMC 1 cut(s) 2762
BseXI GCAGC 9 cut(s) 53, 244, 775, 1305, 1807, 1934, 2255, 3417, 3696
Bsh1236I CGCG 1 cut(s) 3096
BshNI GGYRCC 2 cut(s) 305, 1373
BsiHKAI GWGCWC 5 cut(s) 985, 1404, 2424, 2762, 3078
BsiWI CGTACG 1 cut(s) 3698
BslFI GGGAC 2 cut(s) 202, 2525
BsmAI GTCTC 6 cut(s) 497, 650, 1837, 2673, 2768, 3362
BsmFI GGGAC 2 cut(s) 202, 2525
BsmI GAATGC 2 cut(s) 93, 881
Bsp1286I GDGCHC 6 cut(s) 985, 1404, 2364, 2424, 2762, 3078
Bsp13I TCCGGA 4 cut(s) 370, 2296, 3400, 3676
Bsp1407I TGTACA 1 cut(s) 3228
Bsp1720I GCTNAGC 1 cut(s) 3522
BspACI CCGC 8 cut(s) 34, 64, 480, 1343, 1377, 1429, 1643, 3194
BspEI TCCGGA 4 cut(s) 370, 2296, 3400, 3676
BspFNI CGCG 1 cut(s) 3096
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 9 cut(s) 6, 62, 267, 307, 1375, 1671, 1941, 2361, 3210
BspMAI CTGCAG 2 cut(s) 1138, 1924
BspMI ACCTGC 1 cut(s) 1137
BspOI GCTAGC 1 cut(s) 1310
BspPI GGATC 8 cut(s) 191, 260, 263, 273, 2924, 3107, 3392, 3458
BspQI GCTCTTC 3 cut(s) 131, 1409, 3066
BspT107I GGYRCC 2 cut(s) 305, 1373
BsrBI CCGCTC 1 cut(s) 1429
BsrDI GCAATG 3 cut(s) 2232, 2716, 3105
BsrGI TGTACA 1 cut(s) 3228
BssNI GRCGYC 4 cut(s) 306, 691, 1374, 2975
BssSI CACGAG 1 cut(s) 1490
BssT1I CCWWGG 5 cut(s) 111, 1362, 2220, 2504, 3219
Bst2BI CACGAG 1 cut(s) 1490
Bst2UI CCWGG 6 cut(s) 121, 253, 1852, 2246, 3288, 3456
Bst4CI ACNGT 9 cut(s) 523, 727, 1546, 1599, 2757, 2869, 3164, 3373, 3703
Bst6I CTCTTC 4 cut(s) 131, 1409, 1835, 3066
BstACI GRCGYC 4 cut(s) 306, 691, 1374, 2975
BstAPI GCANNNNNTGC 2 cut(s) 403, 571
BstAUI TGTACA 1 cut(s) 3228
BstC8I GCNNGC 6 cut(s) 919, 1159, 1230, 1308, 1965, 2808
BstDSI CCRYGG 1 cut(s) 8
BstENI CCTNNNNNAGG 1 cut(s) 3155
BstFNI CGCG 1 cut(s) 3096
BstH2I RGCGCY 5 cut(s) 309, 458, 1377, 1801, 3241
BstHHI GCGC 6 cut(s) 308, 457, 1376, 1800, 3098, 3240
BstMAI GTCTC 6 cut(s) 497, 650, 1837, 2673, 2768, 3362
BstMWI GCNNNNNNNGC 8 cut(s) 58, 99, 403, 571, 788, 1163, 1521, 3095
BstNI CCWGG 6 cut(s) 121, 253, 1852, 2246, 3288, 3456
BstNSI RCATGY 6 cut(s) 514, 1710, 2629, 2675, 2810, 3318
BstSFI CTRYAG 3 cut(s) 783, 1134, 1920
BstSLI GKGCMC 1 cut(s) 2762
BstUI CGCG 1 cut(s) 3096
BstV1I GCAGC 9 cut(s) 53, 244, 775, 1305, 1807, 1934, 2255, 3417, 3696
BstV2I GAAGAC 2 cut(s) 1602, 3416
BstX2I RGATCY 2 cut(s) 265, 2398
BstXI CCANNNNNNTGG 1 cut(s) 2835
BstYI RGATCY 2 cut(s) 265, 2398
BtgI CCRYGG 1 cut(s) 8
BtgZI GCGATG 2 cut(s) 1432, 1565
BtsI GCAGTG 2 cut(s) 1131, 2421
BtsIMutI CAGTG 7 cut(s) 418, 1131, 2421, 2531, 2753, 2865, 2945
BveI ACCTGC 1 cut(s) 1137
Cac8I GCNNGC 6 cut(s) 919, 1159, 1230, 1308, 1965, 2808
CciI TCATGA 1 cut(s) 193
CfoI GCGC 6 cut(s) 308, 457, 1376, 1800, 3098, 3240
Cfr13I GGNCC 7 cut(s) 39, 203, 842, 1112, 1670, 2815, 3180
CseI GACGC 2 cut(s) 680, 1331
Csp6I GTAC 6 cut(s) 332, 1600, 2479, 3058, 3229, 3699
CviQI GTAC 6 cut(s) 332, 1600, 2479, 3058, 3229, 3699
DinI GGCGCC 2 cut(s) 307, 1375
DraIII CACNNNGTG 1 cut(s) 2783
DriI GACNNNNNGTC 1 cut(s) 2855
EaeI YGGCCR 4 cut(s) 100, 159, 1434, 1479
Eam1104I CTCTTC 4 cut(s) 131, 1409, 1835, 3066
Eam1105I GACNNNNNGTC 1 cut(s) 2855
EarI CTCTTC 4 cut(s) 131, 1409, 1835, 3066
EciI GGCGGA 1 cut(s) 3209
Ecl136II GAGCTC 4 cut(s) 983, 1402, 2422, 3076
Eco130I CCWWGG 5 cut(s) 111, 1362, 2220, 2504, 3219
Eco147I AGGCCT 1 cut(s) 2562
Eco24I GRGCYC 5 cut(s) 985, 1404, 2364, 2424, 3078
Eco32I GATATC 2 cut(s) 675, 2201
Eco47I GGWCC 1 cut(s) 1112
Eco47III AGCGCT 2 cut(s) 1799, 3239
Eco53kI GAGCTC 4 cut(s) 983, 1402, 2422, 3076
Eco57I CTGAAG 4 cut(s) 462, 1430, 2037, 2064
EcoICRI GAGCTC 4 cut(s) 983, 1402, 2422, 3076
EcoNI CCTNNNNNAGG 1 cut(s) 3155
EcoRII CCWGG 6 cut(s) 119, 251, 1850, 2244, 3286, 3454
EcoRV GATATC 2 cut(s) 675, 2201
EcoT14I CCWWGG 5 cut(s) 111, 1362, 2220, 2504, 3219
EcoT22I ATGCAT 2 cut(s) 862, 3583
EcoT38I GRGCYC 5 cut(s) 985, 1404, 2364, 2424, 3078
EgeI GGCGCC 2 cut(s) 307, 1375
EheI GGCGCC 2 cut(s) 307, 1375
ErhI CCWWGG 5 cut(s) 111, 1362, 2220, 2504, 3219
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 2 cut(s) 202, 2525
FauI CCCGC 1 cut(s) 473
FauNDI CATATG 2 cut(s) 2035, 2830
FbaI TGATCA 2 cut(s) 2323, 2449
FblI GTMKAC 4 cut(s) 634, 1059, 2978, 3660
FriOI GRGCYC 5 cut(s) 985, 1404, 2364, 2424, 3078
FspBI CTAG 5 cut(s) 387, 1127, 1257, 1307, 2382
GlaI GCGC 6 cut(s) 307, 456, 1375, 1799, 3097, 3239
GsuI CTGGAG 3 cut(s) 1518, 2169, 2697
HaeII RGCGCY 5 cut(s) 309, 458, 1377, 1801, 3241
HgaI GACGC 2 cut(s) 680, 1331
HhaI GCGC 6 cut(s) 308, 457, 1376, 1800, 3098, 3240
Hin1I GRCGYC 4 cut(s) 306, 691, 1374, 2975
Hin6I GCGC 6 cut(s) 306, 455, 1374, 1798, 3096, 3238
HinP1I GCGC 6 cut(s) 306, 455, 1374, 1798, 3096, 3238
HincII GTYRAC 5 cut(s) 635, 1060, 1593, 2979, 3661
HindII GTYRAC 5 cut(s) 635, 1060, 1593, 2979, 3661
HindIII AAGCTT 1 cut(s) 3683
HpaI GTTAAC 1 cut(s) 1593
HphI GGTGA 9 cut(s) 593, 836, 884, 1290, 1829, 1922, 2737, 2911, 3007
Hpy166II GTNNAC 9 cut(s) 418, 635, 1060, 1593, 2658, 2760, 2957, 2979, 3661
Hpy8I GTNNAC 9 cut(s) 418, 635, 1060, 1593, 2658, 2760, 2957, 2979, 3661
Hpy99I CGWCG 4 cut(s) 1325, 1373, 2964, 2980
HpyCH4III ACNGT 9 cut(s) 523, 727, 1546, 1599, 2757, 2869, 3164, 3373, 3703
HpyCH4IV ACGT 4 cut(s) 820, 2962, 2975, 2993
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 99, 403, 571, 788, 1163, 1521, 3095
HpySE526I ACGT 4 cut(s) 820, 2962, 2975, 2993
Hsp92I GRCGYC 4 cut(s) 306, 691, 1374, 2975
HspAI GCGC 6 cut(s) 306, 455, 1374, 1798, 3096, 3238
KasI GGCGCC 2 cut(s) 305, 1373
Kpn2I TCCGGA 4 cut(s) 370, 2296, 3400, 3676
Ksp22I TGATCA 2 cut(s) 2323, 2449
KspAI GTTAAC 1 cut(s) 1593
LguI GCTCTTC 3 cut(s) 131, 1409, 3066
Lsp1109I GCAGC 9 cut(s) 53, 244, 775, 1305, 1807, 1934, 2255, 3417, 3696
LweI GCATC 8 cut(s) 547, 1144, 1344, 1502, 1588, 1630, 1886, 3325
MaeI CTAG 5 cut(s) 387, 1127, 1257, 1307, 2382
MaeII ACGT 4 cut(s) 820, 2962, 2975, 2993
MbiI CCGCTC 1 cut(s) 1429
MfeI CAATTG 3 cut(s) 719, 1274, 2344
MflI RGATCY 2 cut(s) 265, 2398
MhlI GDGCHC 6 cut(s) 985, 1404, 2364, 2424, 2762, 3078
Mly113I GGCGCC 2 cut(s) 306, 1374
MlyI GAGTC 8 cut(s) 184, 227, 629, 630, 2537, 3055, 3254, 3667
MmeI TCCRAC 3 cut(s) 126, 207, 1396
Mph1103I ATGCAT 2 cut(s) 862, 3583
MroI TCCGGA 4 cut(s) 370, 2296, 3400, 3676
MroXI GAANNNNTTC 2 cut(s) 1938, 2022
MslI CAYNNNNRTG 6 cut(s) 411, 594, 2148, 2442, 2670, 2870
MspA1I CMGCKG 2 cut(s) 66, 2015
MunI CAATTG 3 cut(s) 719, 1274, 2344
Mva1269I GAATGC 2 cut(s) 93, 881
MvaI CCWGG 6 cut(s) 121, 253, 1852, 2246, 3288, 3456
MvnI CGCG 1 cut(s) 3096
MwoI GCNNNNNNNGC 8 cut(s) 58, 99, 403, 571, 788, 1163, 1521, 3095
NarI GGCGCC 2 cut(s) 306, 1374
NciI CCSGG 6 cut(s) 46, 142, 158, 202, 223, 1628
NdeI CATATG 2 cut(s) 2035, 2830
NheI GCTAGC 1 cut(s) 1306
NlaIV GGNNCC 9 cut(s) 6, 62, 267, 307, 1375, 1671, 1941, 2361, 3210
NmeAIII GCCGAG 6 cut(s) 78, 199, 241, 258, 286, 1405
NmuCI GTSAC 8 cut(s) 1129, 1462, 1817, 2152, 2435, 2529, 2899, 2971
NsiI ATGCAT 2 cut(s) 862, 3583
NspI RCATGY 6 cut(s) 514, 1710, 2629, 2675, 2810, 3318
OliI CACNNNNGTG 2 cut(s) 411, 2148
PaeI GCATGC 1 cut(s) 2810
PagI TCATGA 1 cut(s) 193
PasI CCCWGGG 1 cut(s) 1851
PceI AGGCCT 1 cut(s) 2562
PciI ACATGT 1 cut(s) 2625
PciSI GCTCTTC 3 cut(s) 131, 1409, 3066
PcsI WCGNNNNNNNCGW 1 cut(s) 1377
PctI GAATGC 2 cut(s) 93, 881
PdmI GAANNNNTTC 2 cut(s) 1938, 2022
PfeI GAWTC 8 cut(s) 197, 757, 1471, 2729, 3012, 3037, 3185, 3514
Pfl23II CGTACG 1 cut(s) 3698
PflMI CCANNNNNTGG 1 cut(s) 2675
PfoI TCCNGGA 2 cut(s) 251, 3286
PleI GAGTC 8 cut(s) 184, 226, 628, 630, 2536, 3054, 3254, 3667
PluTI GGCGCC 2 cut(s) 309, 1377
PpsI GAGTC 8 cut(s) 184, 226, 628, 630, 2536, 3054, 3254, 3667
PscI ACATGT 1 cut(s) 2625
PshBI ATTAAT 1 cut(s) 2988
Psp124BI GAGCTC 4 cut(s) 985, 1404, 2424, 3078
Psp6I CCWGG 6 cut(s) 119, 251, 1850, 2244, 3286, 3454
PspGI CCWGG 6 cut(s) 119, 251, 1850, 2244, 3286, 3454
PspLI CGTACG 1 cut(s) 3698
PspN4I GGNNCC 9 cut(s) 6, 62, 267, 307, 1375, 1671, 1941, 2361, 3210
PspPI GGNCC 7 cut(s) 39, 203, 842, 1112, 1670, 2815, 3180
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PstI CTGCAG 2 cut(s) 1138, 1924
PsuI RGATCY 2 cut(s) 265, 2398
PvuII CAGCTG 1 cut(s) 2015
RsaI GTAC 6 cut(s) 333, 1601, 2480, 3059, 3230, 3700
RsaNI GTAC 6 cut(s) 332, 1600, 2479, 3058, 3229, 3699
RseI CAYNNNNRTG 6 cut(s) 411, 594, 2148, 2442, 2670, 2870
SacI GAGCTC 4 cut(s) 985, 1404, 2424, 3078
SalI GTCGAC 4 cut(s) 633, 1058, 2977, 3659
SapI GCTCTTC 3 cut(s) 131, 1409, 3066
Sau96I GGNCC 7 cut(s) 39, 203, 842, 1112, 1670, 2815, 3180
SchI GAGTC 8 cut(s) 184, 227, 629, 630, 2537, 3055, 3254, 3667
SduI GDGCHC 6 cut(s) 985, 1404, 2364, 2424, 2762, 3078
SfaNI GCATC 8 cut(s) 547, 1144, 1344, 1502, 1588, 1630, 1886, 3325
SfcI CTRYAG 3 cut(s) 783, 1134, 1920
SfoI GGCGCC 2 cut(s) 307, 1375
SinI GGWCC 1 cut(s) 1112
SmiMI CAYNNNNRTG 6 cut(s) 411, 594, 2148, 2442, 2670, 2870
SmlI CTYRAG 8 cut(s) 245, 494, 701, 1397, 2146, 2735, 3053, 3377
SmoI CTYRAG 8 cut(s) 245, 494, 701, 1397, 2146, 2735, 3053, 3377
SpeI ACTAGT 1 cut(s) 2381
SphI GCATGC 1 cut(s) 2810
SseBI AGGCCT 1 cut(s) 2562
SsiI CCGC 8 cut(s) 34, 64, 480, 1343, 1377, 1429, 1643, 3194
SspDI GGCGCC 2 cut(s) 305, 1373
SspI AATATT 2 cut(s) 2043, 2843
SspMI CTAG 5 cut(s) 387, 1127, 1257, 1307, 2382
SstI GAGCTC 4 cut(s) 985, 1404, 2424, 3078
StuI AGGCCT 1 cut(s) 2562
StyI CCWWGG 5 cut(s) 111, 1362, 2220, 2504, 3219
TaaI ACNGT 9 cut(s) 523, 727, 1546, 1599, 2757, 2869, 3164, 3373, 3703
TaiI ACGT 4 cut(s) 823, 2965, 2978, 2996
TaqII GACCGA 1 cut(s) 1334
TatI WGTACW 3 cut(s) 1599, 2478, 3228
TauI GCSGC 3 cut(s) 1345, 1379, 1646
TfiI GAWTC 8 cut(s) 197, 757, 1471, 2729, 3012, 3037, 3185, 3514
TscAI CASTG 7 cut(s) 418, 1138, 2421, 2538, 2760, 2872, 2952
TseFI GTSAC 8 cut(s) 1129, 1462, 1817, 2152, 2435, 2529, 2899, 2971
TseI GCWGC 9 cut(s) 66, 232, 788, 1293, 1795, 1922, 2268, 3430, 3709
Tsp45I GTSAC 8 cut(s) 1129, 1462, 1817, 2152, 2435, 2529, 2899, 2971
TspGWI ACGGA 4 cut(s) 61, 306, 476, 1093
TspRI CASTG 7 cut(s) 418, 1138, 2421, 2538, 2760, 2872, 2952
Van91I CCANNNNNTGG 1 cut(s) 2675
VneI GTGCAC 1 cut(s) 2758
VpaK11BI GGWCC 1 cut(s) 1112
VspI ATTAAT 1 cut(s) 2988
XagI CCTNNNNNAGG 1 cut(s) 3155
XapI RAATTY 5 cut(s) 567, 1049, 1694, 2022, 2768
XceI RCATGY 6 cut(s) 514, 1710, 2629, 2675, 2810, 3318
XmiI GTMKAC 4 cut(s) 634, 1059, 2978, 3660
XmnI GAANNNNTTC 2 cut(s) 1938, 2022
XspI CTAG 5 cut(s) 387, 1127, 1257, 1307, 2382
ZraI GACGTC 1 cut(s) 2976
Zsp2I ATGCAT 2 cut(s) 862, 3583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.