Rroxscaffold_4G00325240

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000872
Physical Location & Seq
Reverse (-)
56991690 .. 56992851
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325240.1

Sequence Viewer

Length: 645 bp
ATGGATGCAGTCGATCGAAGCATTGGTAATCTTATATACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCTTCGCCCGCTATTGGCACAGTACATTGTGTCTTCAAAGATTAAAACTCCAGCTCAGTTAGATGCGGCTTTGTTATTTTTTGCAAATACGGGGCCAGGAAACTTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGGCTTATTTGCACATCTTAGGAACAGACATCCGTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAAGTTGGGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAACAAGAAAGAGAAACCTGCTAAAGTAGAGGAGAAAGCTACTGCTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTCAAAAACACTTGAGCTTGACTGGGGGAGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

214

Amino Acids

23.71

Weight (kDa)

5.7

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 4 - 120 1e-38 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 469
AciI CCGC 2 cut(s) 83, 140
AcsI RAATTY 2 cut(s) 191, 495
AcuI CTGAAG 2 cut(s) 510, 537
AfaI GTAC 1 cut(s) 98
AfeI AGCGCT 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 6 cut(s) 111, 181, 197, 221, 266, 611
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 3 cut(s) 128, 482, 624
AluI AGCT 3 cut(s) 128, 482, 624
Aor51HI AGCGCT 1 cut(s) 296
AoxI GGCC 1 cut(s) 167
ApeKI GCWGC 2 cut(s) 292, 419
ApoI RAATTY 2 cut(s) 191, 495
Asp700I GAANNNNTTC 3 cut(s) 435, 495, 606
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 407, 419
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 304, 431
BccI CCATC 1 cut(s) 563
BciT130I CCWGG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 298
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 3 cut(s) 141, 293, 420
BlsI GCNGC 3 cut(s) 142, 294, 421
Bme1390I CCNGG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 168, 438
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 2 cut(s) 127, 383
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 3 cut(s) 10, 343, 374
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 143, 572
BseNI ACTGG 1 cut(s) 634
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 2 cut(s) 304, 431
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 88
BsnI GGCC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 2 cut(s) 83, 140
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 2 cut(s) 142, 573
BspLI GGNNCC 2 cut(s) 168, 438
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 469
BsrI ACTGG 1 cut(s) 634
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 43, 96
Bst6I CTCTTC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 3 cut(s) 129, 332, 581
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 3 cut(s) 10, 343, 374
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 16
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 304, 431
BstV2I GAAGAC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 3 cut(s) 10, 343, 374
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 1 cut(s) 83
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 97
CviAII CATG 1 cut(s) 204
CviJI RGCY 7 cut(s) 128, 143, 169, 318, 353, 482, 624
CviKI_1 RGCY 7 cut(s) 128, 143, 169, 318, 353, 482, 624
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 3 cut(s) 129, 332, 581
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco47III AGCGCT 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 510, 537
EcoRII CCWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 207
FaiI YATR 7 cut(s) 35, 37, 205, 300, 508, 510, 549
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FatI CATG 1 cut(s) 203
FauI CCCGC 1 cut(s) 90
FauNDI CATATG 1 cut(s) 508
Fnu4HI GCNGC 3 cut(s) 141, 293, 420
FokI GGATG 3 cut(s) 17, 330, 361
Fsp4HI GCNGC 3 cut(s) 141, 293, 420
GlaI GCGC 1 cut(s) 296
GluI GCNGC 3 cut(s) 141, 293, 420
GsuI CTGGAG 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 1 cut(s) 169
HhaI GCGC 1 cut(s) 297
Hin1II CATG 1 cut(s) 207
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HphI GGTGA 2 cut(s) 407, 419
Hpy188I TCNGA 2 cut(s) 229, 582
HpyAV CCTTC 1 cut(s) 617
HpyCH4III ACNGT 2 cut(s) 43, 96
HpyCH4V TGCA 6 cut(s) 8, 158, 281, 326, 419, 591
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 3 cut(s) 129, 332, 581
Hsp92II CATG 1 cut(s) 207
HspAI GCGC 1 cut(s) 295
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 7 cut(s) 72, 138, 156, 183, 474, 540, 615
Lsp1109I GCAGC 2 cut(s) 304, 431
LweI GCATC 2 cut(s) 127, 383
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 6 cut(s) 99, 209, 242, 245, 278, 503
MluCI AATT 5 cut(s) 191, 282, 363, 398, 495
MnlI CCTC 4 cut(s) 250, 466, 523, 531
MroXI GAANNNNTTC 3 cut(s) 435, 495, 606
MseI TTAA 4 cut(s) 117, 252, 501, 540
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeI CATATG 1 cut(s) 508
NdeII GATC 1 cut(s) 13
NlaIII CATG 1 cut(s) 207
NlaIV GGNNCC 2 cut(s) 168, 438
NspI RCATGY 1 cut(s) 207
PdmI GAANNNNTTC 3 cut(s) 435, 495, 606
PkrI GCNGC 3 cut(s) 142, 294, 421
Ple19I CGATCG 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 168, 438
PspPI GGNCC 1 cut(s) 167
PstI CTGCAG 1 cut(s) 421
PvuI CGATCG 1 cut(s) 16
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 117, 252, 501, 540
SatI GCNGC 3 cut(s) 141, 293, 420
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 171
SetI ASST 7 cut(s) 130, 261, 438, 463, 484, 546, 626
SfaNI GCATC 2 cut(s) 127, 383
SfcI CTRYAG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 5 cut(s) 191, 282, 363, 398, 495
SsiI CCGC 2 cut(s) 83, 140
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 2 cut(s) 43, 96
TaqI TCGA 2 cut(s) 12, 16
TasI AATT 5 cut(s) 191, 282, 363, 398, 495
TatI WGTACW 1 cut(s) 96
TauI GCSGC 1 cut(s) 143
Tru1I TTAA 4 cut(s) 117, 252, 501, 540
Tru9I TTAA 4 cut(s) 117, 252, 501, 540
TseI GCWGC 2 cut(s) 292, 419
TspDTI ATGAA 2 cut(s) 444, 536
TspGWI ACGGA 1 cut(s) 336
XapI RAATTY 2 cut(s) 191, 495
XceI RCATGY 1 cut(s) 207
XmnI GAANNNNTTC 3 cut(s) 435, 495, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.