RLG00000014023

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000872
Physical Location & Seq
Forward (+)
45869929 .. 45871738
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014023

Sequence Viewer

Length: 732 bp
ATGGATATCTACATACGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCAGGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCCGAGGATAATGAGACTGTCCTAGAGATACGGGTCAAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGTTGCATTGGGTCGCTGAACCTTCTCCTGGGATCGATCCACTCAAAGTGGAAGTCCGATTGTTTGACAGACTATTCAATTCAGAGAATCCTGCTGAGCTGGATGACTGGCTTTCTGATTTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTGCTGTTCCCCCACTTGAGAAAGGTGTTGGTGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCCTGAGAAGCTTGTGTTTAATCGTACGGTCACACTGCGAGATAGCTACAAGAGTGGGAATTTTGTGCATTGTCGTCATGTCATTTGGTATACGAGTGCAGGGGATAGGGCTTTCGGCTTTGTCTCAATTCTGGACCTTCTTGGGTTCCACATCTACACTTCTGCTAGAAGCTTTAGATTTCCCCTTAAACTCCAGATCATTGGTTGGGTGGGAAGTGGCGGCGGAAAATATGGGCTCGAGTTAGAGAGGAGGAGCAATAAAATAGGTCAGGAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

244

Amino Acids

27.42

Weight (kDa)

8.68

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c_C2 PF20974 69 - 147 3.1e-16 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 438, 542
AccIII TCCGGA 1 cut(s) 178
AciI CCGC 2 cut(s) 672, 675
AclWI GGATC 3 cut(s) 170, 236, 245
AcsI RAATTY 1 cut(s) 511
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 1 cut(s) 233
AgsI TTSAA 2 cut(s) 283, 328
AjnI CCWGG 2 cut(s) 64, 232
AluBI AGCT 4 cut(s) 304, 463, 498, 624
AluI AGCT 4 cut(s) 304, 463, 498, 624
Alw26I GTCTC 2 cut(s) 140, 580
AlwI GGATC 3 cut(s) 170, 236, 245
Ama87I CYCGRG 1 cut(s) 689
Aor13HI TCCGGA 1 cut(s) 178
ApoI RAATTY 1 cut(s) 511
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspS9I GGNCC 2 cut(s) 95, 586
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 414
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 2 cut(s) 95, 586
BaeI ACNNNNGTAYC 2 cut(s) 79, 112
BanII GRGCYC 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 66, 234
BcoDI GTCTC 2 cut(s) 140, 580
BfaI CTAG 2 cut(s) 155, 618
BfuAI ACCTGC 1 cut(s) 83
BisI GCNGC 1 cut(s) 673
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 674
Bme1390I CCNGG 2 cut(s) 66, 234
Bme18I GGWCC 2 cut(s) 95, 586
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 2 cut(s) 95, 586
BmiI GGNNCC 2 cut(s) 599, 726
BmrFI CCNGG 2 cut(s) 66, 234
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 135, 233
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 2 cut(s) 66, 234
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 135, 233
BseGI GGATG 3 cut(s) 118, 127, 313
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 3 cut(s) 97, 291, 447
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 2 cut(s) 715, 718
BsgI GTGCAG 1 cut(s) 570
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 1 cut(s) 179
BsiWI CGTACG 1 cut(s) 476
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 140, 580
BsoBI CYCGRG 1 cut(s) 689
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 690
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 4 cut(s) 175, 237, 241, 648
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 2 cut(s) 672, 675
BspCNI CTCAG 3 cut(s) 96, 292, 448
BspDI ATCGAT 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 599, 726
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 3 cut(s) 170, 236, 245
BspT104I TTCGAA 1 cut(s) 414
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 135, 233
BssMI GATC 4 cut(s) 175, 237, 241, 648
BssNAI GTATAC 1 cut(s) 543
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 66, 234
Bst4CI ACNGT 2 cut(s) 151, 481
BstBI TTCGAA 1 cut(s) 414
BstDEI CTNAG 3 cut(s) 83, 300, 456
BstF5I GGATG 3 cut(s) 118, 127, 313
BstKTI GATC 4 cut(s) 178, 240, 244, 651
BstMAI GTCTC 2 cut(s) 140, 580
BstMBI GATC 4 cut(s) 175, 237, 241, 648
BstNI CCWGG 2 cut(s) 66, 234
BstSCI CCNGG 2 cut(s) 64, 232
BstV2I GAAGAC 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 653
BstZ17I GTATAC 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 3 cut(s) 118, 127, 313
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 1 cut(s) 485
BveI ACCTGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 95, 586
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 1 cut(s) 477
CviAII CATG 1 cut(s) 530
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 3 cut(s) 83, 300, 456
DpnI GATC 4 cut(s) 177, 239, 243, 650
DpnII GATC 4 cut(s) 175, 237, 241, 648
EciI GGCGGA 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 690
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 95, 586
Eco88I CYCGRG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 2 cut(s) 64, 232
EcoRV GATATC 1 cut(s) 7
EcoT22I ATGCAT 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 690
FaeI CATG 1 cut(s) 533
FaiI YATR 5 cut(s) 14, 174, 531, 543, 684
FatI CATG 1 cut(s) 529
FblI GTMKAC 2 cut(s) 438, 542
Fnu4HI GCNGC 1 cut(s) 673
FokI GGATG 3 cut(s) 125, 134, 320
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 1 cut(s) 673
FspBI CTAG 2 cut(s) 155, 618
GluI GCNGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 629
HapII CCGG 1 cut(s) 179
Hin1II CATG 1 cut(s) 533
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 2 cut(s) 461, 622
HinfI GANTC 4 cut(s) 38, 292, 446, 451
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 439, 543
Hpy188I TCNGA 6 cut(s) 22, 56, 86, 263, 289, 322
Hpy188III TCNNGA 5 cut(s) 179, 455, 584, 646, 722
Hpy8I GTNNAC 2 cut(s) 439, 543
HpyAV CCTTC 2 cut(s) 237, 599
HpyCH4III ACNGT 2 cut(s) 151, 481
HpyCH4V TGCA 5 cut(s) 116, 211, 359, 520, 551
HpyF3I CTNAG 3 cut(s) 83, 300, 456
Hsp92II CATG 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 178
Kzo9I GATC 4 cut(s) 175, 237, 241, 648
LmnI GCTCC 3 cut(s) 23, 67, 705
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 155, 618
MaeIII GTNAC 2 cut(s) 398, 481
MalI GATC 4 cut(s) 177, 239, 243, 650
MboI GATC 4 cut(s) 175, 237, 241, 648
MboII GAAGA 2 cut(s) 196, 199
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 3 cut(s) 283, 511, 579
MlyI GAGTC 2 cut(s) 32, 445
MnlI CCTC 3 cut(s) 130, 693, 696
Mph1103I ATGCAT 1 cut(s) 361
MroI TCCGGA 1 cut(s) 178
MseI TTAA 3 cut(s) 33, 471, 639
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 66, 234
MvaI CCWGG 2 cut(s) 66, 234
NdeII GATC 4 cut(s) 175, 237, 241, 648
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 2 cut(s) 599, 726
NmeAIII GCCGAG 1 cut(s) 160
NmuCI GTSAC 2 cut(s) 398, 481
NsiI ATGCAT 1 cut(s) 361
NspV TTCGAA 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 689
PfeI GAWTC 2 cut(s) 292, 446
Pfl23II CGTACG 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 64
PkrI GCNGC 1 cut(s) 674
PleI GAGTC 2 cut(s) 32, 445
PpsI GAGTC 2 cut(s) 32, 445
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 64, 232
PspGI CCWGG 2 cut(s) 64, 232
PspLI CGTACG 1 cut(s) 476
PspN4I GGNNCC 2 cut(s) 599, 726
PspPI GGNCC 2 cut(s) 95, 586
PspPPI RGGWCCY 1 cut(s) 95
PspXI VCTCGAGB 1 cut(s) 689
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
SalI GTCGAC 1 cut(s) 437
SaqAI TTAA 3 cut(s) 33, 471, 639
SatI GCNGC 1 cut(s) 673
Sau3AI GATC 4 cut(s) 175, 237, 241, 648
Sau96I GGNCC 2 cut(s) 95, 586
SchI GAGTC 2 cut(s) 32, 445
ScrFI CCNGG 2 cut(s) 66, 234
SduI GDGCHC 1 cut(s) 690
SetI ASST 9 cut(s) 97, 229, 306, 388, 465, 500, 591, 626, 721
SfaNI GCATC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 2 cut(s) 95, 586
SlaI CTCGAG 1 cut(s) 689
SmlI CTYRAG 2 cut(s) 377, 689
SmoI CTYRAG 2 cut(s) 377, 689
Sse9I AATT 3 cut(s) 283, 511, 579
SsiI CCGC 2 cut(s) 672, 675
SspMI CTAG 2 cut(s) 155, 618
StyD4I CCNGG 2 cut(s) 64, 232
TaaI ACNGT 2 cut(s) 151, 481
TaqI TCGA 6 cut(s) 240, 338, 414, 438, 449, 690
TasI AATT 3 cut(s) 283, 511, 579
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 2 cut(s) 292, 446
Tru1I TTAA 3 cut(s) 33, 471, 639
Tru9I TTAA 3 cut(s) 33, 471, 639
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 2 cut(s) 398, 481
Tsp45I GTSAC 2 cut(s) 398, 481
TspRI CASTG 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 95, 586
XapI RAATTY 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 2 cut(s) 438, 542
XspI CTAG 2 cut(s) 155, 618
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.