Rh4BG344200

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000872
Physical Location & Seq
Forward (+)
50666566 .. 50673354
6789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG344200.1

Sequence Viewer

Length: 2388 bp
ATGCCGGCCAAGGATGACGCCGCCGCCGCCGAGAAGGAGAAGAACCTTGAGCTCTTCCTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACCATCGCCAACAACAAGGTCACGGCGAATCTAACGGCCGTCATTGACGAGGCTGGAGTTACTGATGGATGCAGTCGAAGCGTTGGTAATCTTCTATACACGGTTGCTACAAAGTTTCCGGCAAATGCCCTTGTGCATCGCCCCGTGTTAATACAGTACATTGTGTCTTCAAAGATTAAAATCCCGGCTCAGTTAGATGCGGCTTTGTCATATTTTGCGAAAACGGGGCCAGAAAACTTCAAAACAACTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAACAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCCTGGGCTGACCCAAAAATTGTCAAGGCAGCCATTGATACAAACTTGCGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTGTACTCCTGAACAACCAGCTGAAGAAATTCTAAATCCATATGCAATATTCCCTCAGCCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAGCACTTGAGTGTGACTGGGGGCAGAGTTTTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACAGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCACTGAAACTTTTTGAGGATATGAGGCAAGGCCTGATTGAAGAAGGGAAAGCAACTCTAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTTATTGCGTATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAAGTGGTGTATCTATCCGAGTTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATCACCCATTCGCTGTGCACTTTAGAATTTGAGACACGCCGGGCTTCATACTACTGGCTATTGCATGCTTTGGGCCTTTATCAACCATATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGAAAGTTAAATCGACTGGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTGGCTGGTTTACGACGTAGGGGTGTGACGTCGACTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTTGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCACGCACAATGGTTGTGCTTGATCCTGTAAAGGTTGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGATTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGATTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCTGAGGATGATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGGTCGATCCATTCAAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTTCTGTTCCCCCACTTGGGAAAGGTGTTGGTGTTGGCGACAAGTTTCAGTTTGAAAGACTTGGGTATTTTGTGGTCGACAAAGATACGACTCCTGAGAAGCTTGTGTTTAATCGTACAGTCACGCTGCGAGATAGCTACAAGAGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

795

Amino Acids

90.83

Weight (kDa)

6.21

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 14 - 171 7.9e-56 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 174 - 249 6.1e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 273 - 577 2.9e-122 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 580 - 683 2.8e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 693 - 771 1.3e-14 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1628
AccB7I CCANNNNNTGG 1 cut(s) 1325
AccBSI CCGCTC 1 cut(s) 79
AccI GTMKAC 2 cut(s) 1628, 2310
AccIII TCCGGA 2 cut(s) 946, 2050
AciI CCGC 6 cut(s) 21, 24, 27, 79, 293, 1844
AclWI GGATC 4 cut(s) 1574, 1757, 2042, 2108
AcoI YGGCCR 3 cut(s) 6, 84, 129
AcsI RAATTY 3 cut(s) 344, 672, 1418
AcuI CTGAAG 3 cut(s) 80, 687, 714
AcyI GRCGYC 2 cut(s) 18, 1625
AfaI GTAC 6 cut(s) 251, 649, 1130, 1709, 1880, 2350
AfeI AGCGCT 2 cut(s) 449, 1889
AfiI CCNNNNNNNGG 7 cut(s) 871, 1159, 1325, 1807, 2105, 2249, 2250
AflIII ACRYGT 1 cut(s) 1275
AhdI GACNNNNNGTC 1 cut(s) 1505
AhlI ACTAGT 1 cut(s) 1031
AjnI CCWGG 4 cut(s) 500, 894, 1936, 2104
AjuI GAANNNNNNNTTGG 2 cut(s) 2218, 2250
AleI CACNNNNGTG 1 cut(s) 798
Alw21I GWGCWC 4 cut(s) 54, 1074, 1412, 1728
Alw26I GTCTC 4 cut(s) 487, 1323, 1418, 2012
Alw44I GTGCAC 1 cut(s) 1408
AlwI GGATC 4 cut(s) 1574, 1757, 2042, 2108
Aor13HI TCCGGA 2 cut(s) 946, 2050
Aor51HI AGCGCT 2 cut(s) 449, 1889
ApaLI GTGCAC 1 cut(s) 1408
ApeKI GCWGC 6 cut(s) 445, 527, 572, 918, 2080, 2359
ApoI RAATTY 3 cut(s) 344, 672, 1418
ArsI GACNNNNNNTTYG 2 cut(s) 2115, 2147
AseI ATTAAT 1 cut(s) 1638
Asp700I GAANNNNTTC 2 cut(s) 588, 672
AspLEI GCGC 2 cut(s) 450, 1890
AspS9I GGNCC 3 cut(s) 320, 1465, 1830
AsuC2I CCSGG 2 cut(s) 278, 1433
AsuHPI GGTGA 5 cut(s) 479, 572, 1387, 1561, 1657
BaeGI GKGCMC 1 cut(s) 1412
BaeI ACNNNNGTAYC 2 cut(s) 1951, 1984
BanII GRGCYC 4 cut(s) 54, 1014, 1074, 1728
BbsI GAAGAC 2 cut(s) 252, 2066
Bbv12I GWGCWC 4 cut(s) 54, 1074, 1412, 1728
BbvCI CCTCAGC 2 cut(s) 699, 2007
BbvI GCAGC 6 cut(s) 457, 539, 584, 905, 2067, 2346
BccI CCATC 5 cut(s) 104, 152, 740, 994, 1562
BceAI ACGGC 4 cut(s) 99, 116, 132, 144
BciT130I CCWGG 4 cut(s) 502, 896, 1938, 2106
BclI TGATCA 2 cut(s) 973, 1099
BcnI CCSGG 2 cut(s) 278, 1433
BcoDI GTCTC 4 cut(s) 487, 1323, 1418, 2012
BcuI ACTAGT 1 cut(s) 1031
BfaI CTAG 1 cut(s) 1032
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 2 cut(s) 451, 1891
BglII AGATCT 1 cut(s) 1048
BlpI GCTNAGC 1 cut(s) 2172
Bme1390I CCNGG 6 cut(s) 278, 502, 896, 1433, 1938, 2106
BmeRI GACNNNNNGTC 1 cut(s) 1505
BmgT120I GGNCC 3 cut(s) 320, 1465, 1830
BmiI GGNNCC 4 cut(s) 321, 591, 1011, 1860
BmrFI CCNGG 6 cut(s) 278, 502, 896, 1433, 1938, 2106
BmrI ACTGGG 1 cut(s) 816
BmsI GCATC 4 cut(s) 152, 238, 280, 1975
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 2 cut(s) 252, 2066
BplI GAGNNNNNCTC 2 cut(s) 1710, 1742
BpmI CTGGAG 2 cut(s) 168, 1347
Bpu10I CCTNAGC 2 cut(s) 699, 2007
Bpu1102I GCTNAGC 1 cut(s) 2172
BpuEI CTTGAG 5 cut(s) 68, 817, 1406, 1724, 2048
BpuMI CCSGG 2 cut(s) 278, 1433
BsaBI GATNNNNATC 1 cut(s) 272
BsaHI GRCGYC 2 cut(s) 18, 1625
BsaJI CCNNGG 7 cut(s) 9, 500, 501, 870, 1154, 1869, 2105
BsaWI WCCGGW 2 cut(s) 946, 2050
Bsc4I CCNNNNNNNGG 7 cut(s) 871, 1159, 1325, 1807, 2105, 2249, 2250
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 7 cut(s) 75, 811, 1451, 1551, 1602, 1793, 2189
Bse3DI GCAATG 2 cut(s) 882, 1366
Bse8I GATNNNNATC 1 cut(s) 272
BseAI TCCGGA 2 cut(s) 946, 2050
BseBI CCWGG 4 cut(s) 502, 896, 1938, 2106
BseDI CCNNGG 7 cut(s) 9, 500, 501, 870, 1154, 1869, 2105
BseJI GATNNNNATC 1 cut(s) 272
BseLI CCNNNNNNNGG 7 cut(s) 871, 1159, 1325, 1807, 2105, 2249, 2250
BseMI GCAATG 2 cut(s) 882, 1366
BseNI ACTGG 7 cut(s) 75, 811, 1451, 1551, 1602, 1793, 2189
BseRI GAGGAG 1 cut(s) 1302
BseSI GKGCMC 1 cut(s) 1412
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 6 cut(s) 457, 539, 584, 905, 2067, 2346
Bsh1285I CGRYCG 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 4 cut(s) 54, 1074, 1412, 1728
BsiSI CCGG 6 cut(s) 5, 212, 278, 947, 1432, 2051
BslFI GGGAC 1 cut(s) 1175
BslI CCNNNNNNNGG 7 cut(s) 871, 1159, 1325, 1807, 2105, 2249, 2250
BsmAI GTCTC 4 cut(s) 487, 1323, 1418, 2012
BsmFI GGGAC 1 cut(s) 1175
Bsp1286I GDGCHC 5 cut(s) 54, 1014, 1074, 1412, 1728
Bsp13I TCCGGA 2 cut(s) 946, 2050
Bsp1407I TGTACA 1 cut(s) 1878
Bsp143I GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
Bsp1720I GCTNAGC 1 cut(s) 2172
BspACI CCGC 6 cut(s) 21, 24, 27, 79, 293, 1844
BspEI TCCGGA 2 cut(s) 946, 2050
BspLI GGNNCC 4 cut(s) 321, 591, 1011, 1860
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 4 cut(s) 1574, 1757, 2042, 2108
BspQI GCTCTTC 2 cut(s) 59, 1716
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 2 cut(s) 882, 1366
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 1878
BsrI ACTGG 7 cut(s) 75, 811, 1451, 1551, 1602, 1793, 2189
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 7 cut(s) 9, 500, 501, 870, 1154, 1869, 2105
BssMI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
BssNI GRCGYC 2 cut(s) 18, 1625
BssT1I CCWWGG 4 cut(s) 9, 870, 1154, 1869
Bst2UI CCWGG 4 cut(s) 502, 896, 1938, 2106
Bst4CI ACNGT 6 cut(s) 196, 249, 1519, 1814, 2023, 2353
Bst6I CTCTTC 3 cut(s) 59, 485, 1716
BstACI GRCGYC 2 cut(s) 18, 1625
BstAUI TGTACA 1 cut(s) 1878
BstC8I GCNNGC 3 cut(s) 6, 615, 1458
BstENI CCTNNNNNAGG 1 cut(s) 1805
BstH2I RGCGCY 2 cut(s) 451, 1891
BstHHI GCGC 2 cut(s) 450, 1890
BstKTI GATC 9 cut(s) 976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
BstMAI GTCTC 4 cut(s) 487, 1323, 1418, 2012
BstMBI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 26, 171
BstNI CCWGG 4 cut(s) 502, 896, 1938, 2106
BstNSI RCATGY 5 cut(s) 360, 1279, 1325, 1460, 1968
BstSCI CCNGG 6 cut(s) 276, 500, 894, 1431, 1936, 2104
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 1412
BstV1I GCAGC 6 cut(s) 457, 539, 584, 905, 2067, 2346
BstV2I GAAGAC 2 cut(s) 252, 2066
BstX2I RGATCY 1 cut(s) 1048
BstXI CCANNNNNNTGG 1 cut(s) 1485
BstYI RGATCY 1 cut(s) 1048
BstZI CGGCCG 1 cut(s) 129
BtgZI GCGATG 2 cut(s) 82, 215
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 4 cut(s) 1071, 1181, 1515, 1595
Cac8I GCNNGC 3 cut(s) 6, 615, 1458
CfoI GCGC 2 cut(s) 450, 1890
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 320, 1465, 1830
CseI GACGC 1 cut(s) 26
Csp6I GTAC 6 cut(s) 250, 648, 1129, 1708, 1879, 2349
CviAII CATG 6 cut(s) 357, 866, 1276, 1322, 1457, 1965
CviQI GTAC 6 cut(s) 250, 648, 1129, 1708, 1879, 2349
DpnI GATC 9 cut(s) 975, 1022, 1050, 1101, 1581, 1764, 2049, 2115, 2196
DpnII GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
DriI GACNNNNNGTC 1 cut(s) 1505
EaeI YGGCCR 3 cut(s) 6, 84, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 3 cut(s) 59, 485, 1716
Eam1105I GACNNNNNGTC 1 cut(s) 1505
EarI CTCTTC 3 cut(s) 59, 485, 1716
EciI GGCGGA 1 cut(s) 1859
Ecl136II GAGCTC 3 cut(s) 52, 1072, 1726
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 4 cut(s) 9, 870, 1154, 1869
Eco147I AGGCCT 1 cut(s) 1212
Eco24I GRGCYC 4 cut(s) 54, 1014, 1074, 1728
Eco32I GATATC 1 cut(s) 851
Eco47III AGCGCT 2 cut(s) 449, 1889
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 3 cut(s) 52, 1072, 1726
Eco57I CTGAAG 3 cut(s) 80, 687, 714
EcoICRI GAGCTC 3 cut(s) 52, 1072, 1726
EcoNI CCTNNNNNAGG 1 cut(s) 1805
EcoRII CCWGG 4 cut(s) 500, 894, 1936, 2104
EcoRV GATATC 1 cut(s) 851
EcoT14I CCWWGG 4 cut(s) 9, 870, 1154, 1869
EcoT22I ATGCAT 1 cut(s) 2233
EcoT38I GRGCYC 4 cut(s) 54, 1014, 1074, 1728
ErhI CCWWGG 4 cut(s) 9, 870, 1154, 1869
FaeI CATG 6 cut(s) 360, 869, 1279, 1325, 1460, 1968
FaqI GGGAC 1 cut(s) 1175
FatI CATG 6 cut(s) 356, 865, 1275, 1321, 1456, 1964
FauNDI CATATG 2 cut(s) 685, 1480
FbaI TGATCA 2 cut(s) 973, 1099
FblI GTMKAC 2 cut(s) 1628, 2310
FriOI GRGCYC 4 cut(s) 54, 1014, 1074, 1728
FspBI CTAG 1 cut(s) 1032
GlaI GCGC 2 cut(s) 449, 1889
GsuI CTGGAG 2 cut(s) 168, 1347
HaeII RGCGCY 2 cut(s) 451, 1891
HapII CCGG 6 cut(s) 5, 212, 278, 947, 1432, 2051
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 450, 1890
Hin1I GRCGYC 2 cut(s) 18, 1625
Hin1II CATG 6 cut(s) 360, 869, 1279, 1325, 1460, 1968
Hin6I GCGC 2 cut(s) 448, 1888
HinP1I GCGC 2 cut(s) 448, 1888
HincII GTYRAC 2 cut(s) 1629, 2311
HindII GTYRAC 2 cut(s) 1629, 2311
HindIII AAGCTT 1 cut(s) 2333
HpaII CCGG 6 cut(s) 5, 212, 278, 947, 1432, 2051
HphI GGTGA 5 cut(s) 479, 572, 1387, 1561, 1657
Hpy166II GTNNAC 5 cut(s) 1308, 1410, 1607, 1629, 2311
Hpy188III TCNNGA 9 cut(s) 58, 653, 836, 947, 983, 1385, 1497, 2051, 2327
Hpy8I GTNNAC 5 cut(s) 1308, 1410, 1607, 1629, 2311
Hpy99I CGWCG 2 cut(s) 1614, 1630
HpyAV CCTTC 6 cut(s) 28, 55, 955, 1074, 1217, 2109
HpyCH4III ACNGT 6 cut(s) 196, 249, 1519, 1814, 2023, 2353
HpyCH4IV ACGT 4 cut(s) 1095, 1612, 1625, 1643
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 171
HpySE526I ACGT 4 cut(s) 1095, 1612, 1625, 1643
Hsp92I GRCGYC 2 cut(s) 18, 1625
Hsp92II CATG 6 cut(s) 360, 869, 1279, 1325, 1460, 1968
HspAI GCGC 2 cut(s) 448, 1888
Kpn2I TCCGGA 2 cut(s) 946, 2050
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 973, 1099
Kzo9I GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
LguI GCTCTTC 2 cut(s) 59, 1716
LmnI GCTCC 6 cut(s) 442, 1009, 1069, 1744, 1895, 1939
Lsp1109I GCAGC 6 cut(s) 457, 539, 584, 905, 2067, 2346
LweI GCATC 4 cut(s) 152, 238, 280, 1975
MaeI CTAG 1 cut(s) 1032
MaeII ACGT 4 cut(s) 1095, 1612, 1625, 1643
MalI GATC 9 cut(s) 975, 1022, 1050, 1101, 1581, 1764, 2049, 2115, 2196
MbiI CCGCTC 1 cut(s) 79
MboI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
MfeI CAATTG 1 cut(s) 994
MflI RGATCY 1 cut(s) 1048
MhlI GDGCHC 5 cut(s) 54, 1014, 1074, 1412, 1728
MlyI GAGTC 4 cut(s) 1187, 1705, 1904, 2317
MmeI TCCRAC 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 2233
MroI TCCGGA 2 cut(s) 946, 2050
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 588, 672
MslI CAYNNNNRTG 3 cut(s) 798, 1320, 1520
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 6 cut(s) 5, 212, 278, 947, 1432, 2051
MspR9I CCNGG 6 cut(s) 278, 502, 896, 1433, 1938, 2106
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 4 cut(s) 502, 896, 1938, 2106
MwoI GCNNNNNNNGC 2 cut(s) 26, 171
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 278, 1433
NdeI CATATG 2 cut(s) 685, 1480
NdeII GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 360, 869, 1279, 1325, 1460, 1968
NlaIV GGNNCC 4 cut(s) 321, 591, 1011, 1860
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 8 cut(s) 112, 467, 802, 1085, 1179, 1549, 1621, 2353
NsiI ATGCAT 1 cut(s) 2233
NspI RCATGY 5 cut(s) 360, 1279, 1325, 1460, 1968
OliI CACNNNNGTG 1 cut(s) 798
PaeI GCATGC 1 cut(s) 1460
PasI CCCWGGG 1 cut(s) 501
PceI AGGCCT 1 cut(s) 1212
PciI ACATGT 1 cut(s) 1275
PciSI GCTCTTC 2 cut(s) 59, 1716
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 588, 672
PfeI GAWTC 7 cut(s) 121, 1379, 1662, 1687, 1835, 1847, 2164
PflMI CCANNNNNTGG 1 cut(s) 1325
PfoI TCCNGGA 1 cut(s) 1936
PleI GAGTC 4 cut(s) 1186, 1704, 1904, 2317
PpsI GAGTC 4 cut(s) 1186, 1704, 1904, 2317
PscI ACATGT 1 cut(s) 1275
PshBI ATTAAT 1 cut(s) 1638
Psp124BI GAGCTC 3 cut(s) 54, 1074, 1728
Psp6I CCWGG 4 cut(s) 500, 894, 1936, 2104
PspGI CCWGG 4 cut(s) 500, 894, 1936, 2104
PspN4I GGNNCC 4 cut(s) 321, 591, 1011, 1860
PspPI GGNCC 3 cut(s) 320, 1465, 1830
PstI CTGCAG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 1048
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 6 cut(s) 251, 649, 1130, 1709, 1880, 2350
RsaNI GTAC 6 cut(s) 250, 648, 1129, 1708, 1879, 2349
RseI CAYNNNNRTG 3 cut(s) 798, 1320, 1520
SacI GAGCTC 3 cut(s) 54, 1074, 1728
SalI GTCGAC 2 cut(s) 1627, 2309
SapI GCTCTTC 2 cut(s) 59, 1716
Sau3AI GATC 9 cut(s) 973, 1020, 1048, 1099, 1579, 1762, 2047, 2113, 2194
Sau96I GGNCC 3 cut(s) 320, 1465, 1830
SchI GAGTC 4 cut(s) 1187, 1705, 1904, 2317
ScrFI CCNGG 6 cut(s) 278, 502, 896, 1433, 1938, 2106
SduI GDGCHC 5 cut(s) 54, 1014, 1074, 1412, 1728
SfaNI GCATC 4 cut(s) 152, 238, 280, 1975
SfcI CTRYAG 1 cut(s) 570
SmiMI CAYNNNNRTG 3 cut(s) 798, 1320, 1520
SmlI CTYRAG 5 cut(s) 47, 796, 1385, 1703, 2027
SmoI CTYRAG 5 cut(s) 47, 796, 1385, 1703, 2027
SpeI ACTAGT 1 cut(s) 1031
SphI GCATGC 1 cut(s) 1460
SseBI AGGCCT 1 cut(s) 1212
SsiI CCGC 6 cut(s) 21, 24, 27, 79, 293, 1844
SspI AATATT 2 cut(s) 693, 1493
SspMI CTAG 1 cut(s) 1032
SstI GAGCTC 3 cut(s) 54, 1074, 1728
StuI AGGCCT 1 cut(s) 1212
StyD4I CCNGG 6 cut(s) 276, 500, 894, 1431, 1936, 2104
StyI CCWWGG 4 cut(s) 9, 870, 1154, 1869
TaaI ACNGT 6 cut(s) 196, 249, 1519, 1814, 2023, 2353
TaiI ACGT 4 cut(s) 1098, 1615, 1628, 1646
TaqI TCGA 8 cut(s) 169, 1526, 1543, 1628, 2040, 2112, 2210, 2310
TatI WGTACW 4 cut(s) 249, 647, 1128, 1878
TauI GCSGC 4 cut(s) 23, 26, 29, 296
TfiI GAWTC 7 cut(s) 121, 1379, 1662, 1687, 1835, 1847, 2164
TscAI CASTG 4 cut(s) 1071, 1188, 1522, 1602
TseFI GTSAC 8 cut(s) 112, 467, 802, 1085, 1179, 1549, 1621, 2353
TseI GCWGC 6 cut(s) 445, 527, 572, 918, 2080, 2359
Tsp45I GTSAC 8 cut(s) 112, 467, 802, 1085, 1179, 1549, 1621, 2353
TspDTI ATGAA 7 cut(s) 597, 713, 1071, 1160, 1259, 1428, 1679
TspRI CASTG 4 cut(s) 1071, 1188, 1522, 1602
Van91I CCANNNNNTGG 1 cut(s) 1325
VneI GTGCAC 1 cut(s) 1408
VspI ATTAAT 1 cut(s) 1638
XagI CCTNNNNNAGG 1 cut(s) 1805
XapI RAATTY 3 cut(s) 344, 672, 1418
XceI RCATGY 5 cut(s) 360, 1279, 1325, 1460, 1968
XmiI GTMKAC 2 cut(s) 1628, 2310
XmnI GAANNNNTTC 2 cut(s) 588, 672
XspI CTAG 1 cut(s) 1032
ZraI GACGTC 1 cut(s) 1626
Zsp2I ATGCAT 1 cut(s) 2233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.