Rroxscaffold_4G00325440

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000872
Physical Location & Seq
Reverse (-)
57168319 .. 57168961
643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325440.1

Sequence Viewer

Length: 495 bp
ATGCGCGCCAAGGAGAACATCCTTGAGCTCATCTTGAAGGTCGAACTGGACGAGCGGACAACCAAAAACACAATCGCCACCAACAGGGTCACGGCCAATCTGACGGCCGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGAAGCATTGAAAACTTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGCTTTTGAAGAGAATAAATCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGGCTTATCGGCACATCTTAGGAAGAGACATTCGTGGGCTGACCCAAAATTGTTAAGGCAATCATTGATACAAAGTTGCGTGAATTACTTGGTTAAAGGACTGCATGTAATACCCCGAAAATTCAAATTATTTTCCGAAGACATTTTGGAAATGATTTTGTGGCCATGGGCGAAAGTACGAAGGTTGGAGGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

164

Amino Acids

18.78

Weight (kDa)

6.45

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 52 - 114 4.8e-20 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 55
AccII CGCG 1 cut(s) 6
AciI CCGC 1 cut(s) 55
AcoI YGGCCR 3 cut(s) 93, 105, 458
AcsI RAATTY 2 cut(s) 173, 416
AfaI GTAC 1 cut(s) 474
AfeI AGCGCT 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 7 cut(s) 37, 155, 163, 179, 203, 248, 421
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 316
AlwNI CAGNNNCTG 1 cut(s) 131
Aor51HI AGCGCT 1 cut(s) 278
AoxI GGCC 3 cut(s) 93, 105, 458
ApeKI GCWGC 2 cut(s) 122, 274
ApoI RAATTY 2 cut(s) 173, 416
AspLEI GCGC 3 cut(s) 6, 8, 279
BalI TGGCCA 1 cut(s) 460
BanII GRGCYC 1 cut(s) 30
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 109, 286
BccI CCATC 1 cut(s) 128
BceAI ACGGC 3 cut(s) 92, 108, 120
BcoDI GTCTC 1 cut(s) 316
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 280
BisI GCNGC 2 cut(s) 123, 275
BlsI GCNGC 2 cut(s) 124, 276
BmsI GCATC 1 cut(s) 128
BoxI GACNNNNGTC 1 cut(s) 107
BpiI GAAGAC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 9, 461
BsaXI ACNNNNNCTCC 1 cut(s) 476
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse1I ACTGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 9, 461
BseGI GGATG 2 cut(s) 18, 143
BseLI CCNNNNNNNGG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 51
BsePI GCGCGC 1 cut(s) 4
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 109, 286
Bsh1236I CGCG 1 cut(s) 6
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 95, 107, 460
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 3 cut(s) 95, 107, 460
Bsp1286I GDGCHC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 461
BspACI CCGC 1 cut(s) 55
BspANI GGCC 3 cut(s) 95, 107, 460
BspFNI CGCG 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 127
BsrBI CCGCTC 1 cut(s) 55
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 9, 461
BssHII GCGCGC 1 cut(s) 4
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 9, 461
Bst2BI CACGAG 1 cut(s) 116
Bst6I CTCTTC 2 cut(s) 243, 314
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 18, 143
BstFNI CGCG 1 cut(s) 6
BstH2I RGCGCY 1 cut(s) 280
BstHHI GCGC 3 cut(s) 6, 8, 279
BstMAI GTCTC 1 cut(s) 316
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNSI RCATGY 2 cut(s) 189, 404
BstPAI GACNNNNGTC 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 123
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 109, 286
BstV2I GAAGAC 1 cut(s) 441
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 3 cut(s) 95, 107, 460
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 2 cut(s) 18, 143
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 3 cut(s) 6, 8, 279
Csp6I GTAC 1 cut(s) 473
CviAII CATG 3 cut(s) 186, 401, 462
CviJI RGCY 8 cut(s) 28, 95, 107, 122, 242, 300, 335, 460
CviKI_1 RGCY 8 cut(s) 28, 95, 107, 122, 242, 300, 335, 460
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 1 cut(s) 314
EaeI YGGCCR 3 cut(s) 93, 105, 458
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 2 cut(s) 243, 314
EarI CTCTTC 2 cut(s) 243, 314
Ecl136II GAGCTC 1 cut(s) 28
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 9, 461
Eco24I GRGCYC 1 cut(s) 30
Eco47III AGCGCT 1 cut(s) 278
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 9, 461
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 9, 461
FaeI CATG 3 cut(s) 189, 404, 465
FaiI YATR 4 cut(s) 187, 282, 402, 463
FatI CATG 3 cut(s) 185, 400, 461
Fnu4HI GCNGC 2 cut(s) 123, 275
FokI GGATG 2 cut(s) 5, 150
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 123, 275
GlaI GCGC 3 cut(s) 5, 7, 278
GluI GCNGC 2 cut(s) 123, 275
HaeII RGCGCY 1 cut(s) 280
HaeIII GGCC 3 cut(s) 95, 107, 460
HhaI GCGC 3 cut(s) 6, 8, 279
Hin1II CATG 3 cut(s) 189, 404, 465
Hin6I GCGC 3 cut(s) 4, 6, 277
HinP1I GCGC 3 cut(s) 4, 6, 277
Hpy188I TCNGA 3 cut(s) 102, 211, 433
Hpy188III TCNNGA 2 cut(s) 34, 116
HpyAV CCTTC 2 cut(s) 31, 471
HpyCH4V TGCA 4 cut(s) 125, 141, 263, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 1 cut(s) 314
Hsp92II CATG 3 cut(s) 189, 404, 465
HspAI GCGC 3 cut(s) 4, 6, 277
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 2 cut(s) 32, 70
Lsp1109I GCAGC 2 cut(s) 109, 286
LweI GCATC 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 88, 127
MbiI CCGCTC 1 cut(s) 55
MboII GAAGA 6 cut(s) 191, 224, 227, 260, 331, 446
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 460
MluCI AATT 6 cut(s) 173, 264, 344, 379, 416, 422
MluNI TGGCCA 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 3 cut(s) 112, 232, 478
Mox20I TGGCCA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 460
MseI TTAA 3 cut(s) 234, 350, 390
Msp20I TGGCCA 1 cut(s) 460
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 1 cut(s) 147
NcoI CCATGG 1 cut(s) 461
NlaIII CATG 3 cut(s) 189, 404, 465
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 2 cut(s) 189, 404
PauI GCGCGC 1 cut(s) 4
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PkrI GCNGC 2 cut(s) 124, 276
PshAI GACNNNNGTC 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 30
PstI CTGCAG 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 131
PteI GCGCGC 1 cut(s) 4
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 3 cut(s) 234, 350, 390
SatI GCNGC 2 cut(s) 123, 275
SduI GDGCHC 1 cut(s) 30
SetI ASST 3 cut(s) 30, 42, 482
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 6 cut(s) 173, 264, 344, 379, 416, 422
SsiI CCGC 1 cut(s) 55
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 2 cut(s) 9, 461
TaqI TCGA 2 cut(s) 42, 145
TasI AATT 6 cut(s) 173, 264, 344, 379, 416, 422
Tru1I TTAA 3 cut(s) 234, 350, 390
Tru9I TTAA 3 cut(s) 234, 350, 390
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 122, 274
Tsp45I GTSAC 1 cut(s) 88
XapI RAATTY 2 cut(s) 173, 416
XceI RCATGY 2 cut(s) 189, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.