Rw6G013340

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000872
Physical Location & Seq
Forward (+)
24614218 .. 24616012
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013340.1

Sequence Viewer

Length: 423 bp
ATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCACACAATGGTTGTGCTTGATCTTGTAAAGATCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGACTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCAATTTTCGCCTAAAGACTCAAAATACTACTTCGGACTTGCTCCTGGAGAATCTGCCCTGCTCAGACACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACAGGTCGAGTATGATCAGGAGAAGAAGACAAAGCCAAAGGAATTTGGAGATATTGCTAGATATGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

140

Amino Acids

16.53

Weight (kDa)

5.4

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c_C PF03950 19 - 76 2.1e-09 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AciI CCGC 1 cut(s) 161
AcsI RAATTY 1 cut(s) 392
AfaI GTAC 2 cut(s) 26, 197
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 124
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 3 cut(s) 43, 56, 323
AluI AGCT 3 cut(s) 43, 56, 323
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 20
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 1 cut(s) 146
ApoI RAATTY 1 cut(s) 392
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 20
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 45
BbvCI CCTCAGC 1 cut(s) 324
BciT130I CCWGG 1 cut(s) 254
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 408
BfoI RGCGCY 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 254
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 383
BplI GAGNNNNNCTC 2 cut(s) 27, 59
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 110
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 306, 315
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 3 cut(s) 147, 285, 315
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 1 cut(s) 48
BshFI GGCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 3 cut(s) 79, 90, 364
BspACI CCGC 1 cut(s) 161
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 3 cut(s) 148, 284, 316
BspLI GGNNCC 1 cut(s) 177
BspQI GCTCTTC 1 cut(s) 33
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 79, 90, 364
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 2 cut(s) 131, 340
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 195
BstDEI CTNAG 4 cut(s) 11, 156, 271, 324
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 306, 315
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 82, 93, 367
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 79, 90, 364
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 383
BsuRI GGCC 1 cut(s) 148
BtsCI GGATG 2 cut(s) 306, 315
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 25, 196
CviAII CATG 1 cut(s) 281
CviJI RGCY 5 cut(s) 43, 56, 148, 323, 385
CviKI_1 RGCY 5 cut(s) 43, 56, 148, 323, 385
CviQI GTAC 2 cut(s) 25, 196
DdeI CTNAG 4 cut(s) 11, 156, 271, 324
DpnI GATC 3 cut(s) 81, 92, 366
DpnII GATC 3 cut(s) 79, 90, 364
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 45
Eco47III AGCGCT 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 284
FaiI YATR 4 cut(s) 122, 282, 363, 414
FatI CATG 1 cut(s) 280
FbaI TGATCA 1 cut(s) 364
FokI GGATG 2 cut(s) 313, 322
FriOI GRGCYC 1 cut(s) 45
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 148
HhaI GCGC 1 cut(s) 207
Hin1II CATG 1 cut(s) 284
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 6 cut(s) 4, 13, 152, 164, 226, 259
Hpy188I TCNGA 6 cut(s) 12, 35, 157, 244, 274, 422
Hpy188III TCNNGA 1 cut(s) 368
HpyCH4III ACNGT 2 cut(s) 131, 340
HpyCH4V TGCA 1 cut(s) 304
HpyF3I CTNAG 4 cut(s) 11, 156, 271, 324
Hsp92II CATG 1 cut(s) 284
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 3 cut(s) 79, 90, 364
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 3 cut(s) 61, 212, 255
LpnPI CCDG 6 cut(s) 91, 239, 266, 280, 338, 353
LweI GCATC 1 cut(s) 291
MaeI CTAG 1 cut(s) 408
MalI GATC 3 cut(s) 81, 92, 366
MboI GATC 3 cut(s) 79, 90, 364
MboII GAAGA 3 cut(s) 50, 385, 388
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 2 cut(s) 211, 392
MlyI GAGTC 4 cut(s) 22, 146, 173, 220
MnlI CCTC 5 cut(s) 29, 69, 128, 151, 319
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
NdeII GATC 3 cut(s) 79, 90, 364
NlaIII CATG 1 cut(s) 284
NlaIV GGNNCC 1 cut(s) 177
NspI RCATGY 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 4, 259
PfoI TCCNGGA 1 cut(s) 252
PleI GAGTC 4 cut(s) 21, 146, 172, 220
PpsI GAGTC 4 cut(s) 21, 146, 172, 220
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 1 cut(s) 147
PspXI VCTCGAGB 1 cut(s) 20
RsaI GTAC 2 cut(s) 26, 197
RsaNI GTAC 2 cut(s) 25, 196
SacI GAGCTC 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 33
Sau3AI GATC 3 cut(s) 79, 90, 364
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 4 cut(s) 22, 146, 173, 220
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 1 cut(s) 45
SetI ASST 7 cut(s) 45, 58, 120, 177, 192, 325, 357
SfaNI GCATC 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 20, 344
SmoI CTYRAG 2 cut(s) 20, 344
Sse9I AATT 2 cut(s) 211, 392
SsiI CCGC 1 cut(s) 161
SspMI CTAG 1 cut(s) 408
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 131, 340
TaqI TCGA 2 cut(s) 21, 357
TasI AATT 2 cut(s) 211, 392
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 2 cut(s) 4, 259
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 1 cut(s) 392
XceI RCATGY 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.