AT5G19720

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000872
Physical Location & Seq
Forward (+)
6667664 .. 6668700
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19720.1

Sequence Viewer

Length: 513 bp
ATGGAGCTTGATGCTAAAAGATGGCCTGATGCTCCGAATGATGATCCCTCAGCATTCTACAAGGTTCCGCTCTCTAGAGTTATATACATGGAGCAATCTGACTTCCAAAATGAGGATTCGAAAAATTATTATGGACTTGCCCCTGGTAAATCAGTCTTGCTTCGATACACTTTCCCAATCAAGTGCACCAACGTTGTCTTTGCTGATGACACTAAAACTGTTTGTGAGATTTATGTGGAGTATGATCCCGAGAAGAAGATAAAGCCAAAGGGTGTTCTACACTGGGTCCCTGAATATTCACCAGGAAAAGAGCCAACTAAGGTTGAAGTCTGTTCATTTGAGAACCTCTTTAACTCCGAGAACCCAGCTGAACTCAATGATGATTGGCTCACTGACATTAACCCCCAACTCCAAAGTGGTTATTACACGGTTGACAAAGACTCTATCCCGGGAAAGCTTGTGTTTAACCGGACGGTCACACTCAAAGATGGCTACAAGAAAGGTGGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

170

Amino Acids

19.48

Weight (kDa)

5.01

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c_C PF03950 7 - 82 5.3e-10 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 1 cut(s) 68
AclI AACGTT 1 cut(s) 192
AclWI GGATC 2 cut(s) 38, 239
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 1 cut(s) 326
AjnI CCWGG 2 cut(s) 142, 301
AluBI AGCT 3 cut(s) 7, 368, 457
AluI AGCT 3 cut(s) 7, 368, 457
Alw21I GWGCWC 1 cut(s) 188
Alw44I GTGCAC 1 cut(s) 184
AlwI GGATC 2 cut(s) 38, 239
Ama87I CYCGRG 2 cut(s) 248, 448
AoxI GGCC 1 cut(s) 23
ApaLI GTGCAC 1 cut(s) 184
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 2 cut(s) 449, 450
AsuHPI GGTGA 1 cut(s) 291
AsuII TTCGAA 1 cut(s) 119
AvaI CYCGRG 2 cut(s) 248, 448
AvaII GGWCC 1 cut(s) 286
BaeGI GKGCMC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 188
BbvCI CCTCAGC 1 cut(s) 49
BccI CCATC 2 cut(s) 15, 482
BciT130I CCWGG 2 cut(s) 144, 303
BcnI CCSGG 2 cut(s) 449, 450
BfaI CTAG 1 cut(s) 75
Bme1390I CCNGG 4 cut(s) 144, 303, 449, 450
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 2 cut(s) 248, 448
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 66, 287, 288
BmrFI CCNGG 4 cut(s) 144, 303, 449, 450
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 292
Bpu10I CCTNAGC 1 cut(s) 49
Bpu14I TTCGAA 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 449, 450
BsaJI CCNNGG 2 cut(s) 142, 448
BsaWI WCCGGW 1 cut(s) 468
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 2 cut(s) 144, 303
BseDI CCNNGG 2 cut(s) 142, 448
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 188
BseYI CCCAGC 1 cut(s) 364
BshFI GGCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 2 cut(s) 248, 448
BsiSI CCGG 2 cut(s) 449, 469
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 53
BsnI GGCC 1 cut(s) 25
BsoBI CYCGRG 2 cut(s) 248, 448
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 2 cut(s) 43, 244
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 66, 287, 288
BspPI GGATC 2 cut(s) 38, 239
BspT104I TTCGAA 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 70
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 142, 448
BssMI GATC 2 cut(s) 43, 244
Bst2UI CCWGG 2 cut(s) 144, 303
Bst4CI ACNGT 3 cut(s) 220, 430, 475
BstBI TTCGAA 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 49, 318
BstKTI GATC 2 cut(s) 46, 247
BstMBI GATC 2 cut(s) 43, 244
BstNI CCWGG 2 cut(s) 144, 303
BstSCI CCNGG 4 cut(s) 142, 301, 447, 448
BstSLI GKGCMC 1 cut(s) 188
BsuRI GGCC 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 280, 390
Cfr13I GGNCC 1 cut(s) 286
Cfr9I CCCGGG 1 cut(s) 448
CspCI CAANNNNNGTGG 1 cut(s) 484
CviAII CATG 1 cut(s) 88
CviJI RGCY 8 cut(s) 7, 25, 265, 313, 368, 388, 457, 492
CviKI_1 RGCY 8 cut(s) 7, 25, 265, 313, 368, 388, 457, 492
DdeI CTNAG 2 cut(s) 49, 318
DpnI GATC 2 cut(s) 45, 246
DpnII GATC 2 cut(s) 43, 244
Eco47I GGWCC 1 cut(s) 286
Eco88I CYCGRG 2 cut(s) 248, 448
EcoO109I RGGNCCY 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 142, 301
FaeI CATG 1 cut(s) 91
FaiI YATR 6 cut(s) 83, 85, 89, 132, 234, 243
FaqI GGGAC 1 cut(s) 272
FatI CATG 1 cut(s) 87
FspBI CTAG 1 cut(s) 75
GsaI CCCAGC 1 cut(s) 368
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 449, 469
Hin1II CATG 1 cut(s) 91
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 2 cut(s) 116, 440
HpaII CCGG 2 cut(s) 449, 469
HphI GGTGA 1 cut(s) 291
Hpy166II GTNNAC 2 cut(s) 186, 433
Hpy188I TCNGA 3 cut(s) 36, 100, 358
Hpy188III TCNNGA 2 cut(s) 75, 248
Hpy8I GTNNAC 2 cut(s) 186, 433
HpyCH4III ACNGT 3 cut(s) 220, 430, 475
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 1 cut(s) 186
HpyF3I CTNAG 2 cut(s) 49, 318
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 1 cut(s) 91
KflI GGGWCCC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 43, 244
LmnI GCTCC 3 cut(s) 4, 37, 91
LweI GCATC 1 cut(s) 19
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 45, 246
MbiI CCGCTC 1 cut(s) 70
MboI GATC 2 cut(s) 43, 244
MboII GAAGA 2 cut(s) 265, 268
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 1 cut(s) 124
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 3 cut(s) 58, 106, 356
MseI TTAA 3 cut(s) 351, 399, 465
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 2 cut(s) 449, 469
MspR9I CCNGG 4 cut(s) 144, 303, 449, 450
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 2 cut(s) 144, 303
NciI CCSGG 2 cut(s) 449, 450
NdeII GATC 2 cut(s) 43, 244
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 3 cut(s) 66, 287, 288
NmuCI GTSAC 1 cut(s) 475
NspV TTCGAA 1 cut(s) 119
PctI GAATGC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 116
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 286
Psp6I CCWGG 2 cut(s) 142, 301
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 2 cut(s) 142, 301
PspN4I GGNNCC 3 cut(s) 66, 287, 288
PspPI GGNCC 1 cut(s) 286
PspPPI RGGWCCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 368
SaqAI TTAA 3 cut(s) 351, 399, 465
Sau3AI GATC 2 cut(s) 43, 244
Sau96I GGNCC 1 cut(s) 286
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 4 cut(s) 144, 303, 449, 450
SduI GDGCHC 1 cut(s) 188
SetI ASST 8 cut(s) 9, 66, 195, 324, 348, 370, 459, 505
SfaNI GCATC 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 1 cut(s) 286
SmaI CCCGGG 1 cut(s) 450
Sse9I AATT 1 cut(s) 124
SsiI CCGC 1 cut(s) 68
SspI AATATT 1 cut(s) 296
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 4 cut(s) 142, 301, 447, 448
TaaI ACNGT 3 cut(s) 220, 430, 475
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 119, 163
TasI AATT 1 cut(s) 124
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 3 cut(s) 351, 399, 465
Tru9I TTAA 3 cut(s) 351, 399, 465
TscAI CASTG 2 cut(s) 287, 397
TseFI GTSAC 1 cut(s) 475
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 324
TspMI CCCGGG 1 cut(s) 448
TspRI CASTG 2 cut(s) 287, 397
VneI GTGCAC 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 286
XbaI TCTAGA 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XmaI CCCGGG 1 cut(s) 448
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.