Prupe.1G313400_v2.0.a1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000872
Physical Location & Seq
Reverse (-)
30350735 .. 30358242
7508 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G313400.1

Sequence Viewer

Length: 2388 bp
ATGCCAGGCAAGGACGATGGCGGCTCCGATAAGGAGAAGAACCTGGAGCTCTTTTTGAAGGTCGGGTTGGATGAGCGAACGGCCAAAAACACCATCGCCAACAACAAAGTCACAGCTAATCTCACTGCTGTCATTCACGAGGCCGCAGTTACTGATGGATGCAGTCGAACTGTTGGTAATCTCCTGTATACGGTTGCCACGAAGCACCCAGCAAATGCCCTTCTGCATCGCCCAACGTTGGTGCAATACATTGTATCATCGAAGATCAAAACTCCAGCCCAGTTAGAAGCGGCGTTTTCATTTTTTGCAACTACAGGGCCAGAGAATTTCAAACTAAGCGAATTTGAGGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGTAGAAGAAATAAAACAAACTGTTAATGAGGTTTTTGAAGAGAATAAGACTGCAATTTTGGAGCAGCGCTACAGAACAAATGTCGGTGATTTATTTGCACATGTTAGGAAGAGGCATCCATGGGCTGATCCAAAAATTGTCAAGCAATTCATTGATGAAAAATTGCATGAGTTACTTGGTGAAAGGACTGCAGCAGATAGTGAAAAGGTTCCTAAAAAAAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCCATTGCTGTTTCTACTCCCGAACAGCCATCTGAAGAAGTTCTTAATCCATTTTTGATATTCCCTCAACCGGAGGAAAATATTAAGGTGCATACTTCAGTTTTCTTTAGTGATGGATCTATTCTGAGATGTTGTAACACAAAGGAACTGCTTGAGAAACACTTAAGTGTGACCGGAGGGAAAGTTTTGACCCGCTTTCCTCCTGAACCAAATGGATATCTGCATATAGGTCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGAGGCTGCTACCTGCGGTTTGATGATACAAACCCGGAAGCTGAAAAGAAAGAATATATTGATCATATTCAGGAAATTGTAAAGTGGATGGGTTGGAAGCCCTACAAGATCACTTACACTAGTGATTACTTTCAAGATCTGTATGATTTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTATGTTGATCATCAGACACCTGACGATATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTGCAGAATCATTGAAACTTTTTGAGGATATGAGACGAGGCCTGATTGAAGAAGGCAAAGCAACCCTTAGAATGAAGCAAGACATGCAGAGCGATAATTTTAATATGTATGACCTTATTGCATATCGTATCAAGTTTACTCCTCACCCGCATGCTGGAGACAAGTGGTGTATCTATCCAAGTTATGATTATGCTCACTGCATTGTGGATTCTCTTGAGAATATCACACATTCGCTGTGCACACTTGAATTTGAAACACGCCGTGCTTCATACTACTGGTTATTGCACGCACTTGGAATTTATCAACCATATGTATGGGAATACTCACGACTGAATATCAGTAACACCGTTATGTCAAAGCGTAAGTTAAATCGACTGGTGACAGAGAAGTGGGTTGATGGTTGGGATGACCCACGTCTTATGACACTGGCTGGTTTACGACGTAGGGGTGTAACATCTACTGCAATAAATGCTTTTGTTAGAGGAATTGGAATCACTAGAAGTGATTGTAGTATGATACATTTAAGTCGCCTCGAGTATCACATAAGAGAGGAACTAAATAAAACAGCTCCTCGCTCAATAGCTGTGCTACATCCCCTCAAGGTGGTTATTACTAACCTGGAAGCTAAAACTACAATGGATCTAGAAGCAAGGAAATGGCCTGATGCTCAGACAGATGATGCATCTGCTTTCTACAAGGTCCCCTTTTCCAATACTGTTTATATTGAGAGGTCTGATTTTCGGCTTAAAGATTCAAAAGATTACTTTGGGCTTGCTCCAGGAAAATCAGTCCTACTCAGGTATGCGTTCCCTATTAAGTGCACAGATGTCATATTAGCTGATGATAAGGAAACTGTTCTTGAGATACGGGCTGAGTATGATCCTACAAAGAAGACAAAGCCAAAGGGGGTGCTTCATTGGGTTGCTGAACCTTCCCCAGGAGTTGATCCACTCAAAGTGGAAATCAGATTGTTTGACAGACTGTTCCTTTCAGAGAATCCTGCTGAGCTCGATGATTGGCTTGCTGATCTGAACCCACAATCCAAAGTGGTAATACCCGATGCATATGCTGTGCCCTCACTTCGGGGTGCTGTTGTTGGGGACACATTTCAGTTTGAAAGGCTTGGCTATTTTTCAGTCGATAAAGATTCGACTCCTGAAAAACTTGTGTTTAATCGTACCGTGACACTGCGAGATAGCTATCATAAGGGTGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

796

Amino Acids

90.86

Weight (kDa)

6.67

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 930
AccI GTMKAC 1 cut(s) 188
AciI CCGC 6 cut(s) 21, 144, 290, 823, 925, 1316
AclI AACGTT 1 cut(s) 236
AclWI GGATC 5 cut(s) 500, 754, 1815, 2042, 2108
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 4 cut(s) 325, 341, 1415, 1464
AcuI CTGAAG 2 cut(s) 684, 711
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 2 cut(s) 1127, 2347
AfeI AGCGCT 1 cut(s) 446
AfiI CCNNNNNNNGG 8 cut(s) 190, 238, 700, 1156, 1322, 1771, 2105, 2250
AflII CTTAAG 1 cut(s) 793
AflIII ACRYGT 1 cut(s) 478
AhlI ACTAGT 1 cut(s) 1028
AjiI CACGTC 1 cut(s) 1583
AjnI CCWGG 6 cut(s) 4, 42, 891, 1785, 1945, 2104
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AleI CACNNNNGTG 1 cut(s) 795
AloI GAACNNNNNNTCC 2 cut(s) 2192, 2224
Alw21I GWGCWC 5 cut(s) 51, 1071, 1409, 1991, 2178
Alw26I GTCTC 2 cut(s) 1195, 1320
Alw44I GTGCAC 2 cut(s) 1405, 1987
AlwI GGATC 5 cut(s) 500, 754, 1815, 2042, 2108
AlwNI CAGNNNCTG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 1700
Aor51HI AGCGCT 1 cut(s) 446
AoxI GGCC 7 cut(s) 81, 141, 317, 889, 1163, 1207, 1826
ApaLI GTGCAC 2 cut(s) 1405, 1987
ApeKI GCWGC 3 cut(s) 442, 569, 915
ApoI RAATTY 4 cut(s) 325, 341, 1415, 1464
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
Asp700I GAANNNNTTC 3 cut(s) 585, 669, 2022
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 317, 1867
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 4 cut(s) 476, 569, 1304, 1558
AvaI CYCGRG 1 cut(s) 1700
AvaII GGWCC 1 cut(s) 1867
BaeGI GKGCMC 3 cut(s) 1409, 1991, 2244
BanII GRGCYC 3 cut(s) 51, 1071, 2178
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 2066
Bbv12I GWGCWC 5 cut(s) 51, 1071, 1409, 1991, 2178
BbvI GCAGC 3 cut(s) 454, 581, 902
BccI CCATC 7 cut(s) 11, 101, 149, 667, 737, 991, 1559
BceAI ACGGC 2 cut(s) 96, 1413
BcgI CGANNNNNNTGC 2 cut(s) 2216, 2250
BciT130I CCWGG 6 cut(s) 6, 44, 893, 1787, 1947, 2106
BclI TGATCA 2 cut(s) 970, 1096
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 2 cut(s) 1195, 1320
BcuI ACTAGT 1 cut(s) 1028
BfaI CTAG 3 cut(s) 1029, 1665, 1811
BfmI CTRYAG 3 cut(s) 312, 448, 567
BfoI RGCGCY 1 cut(s) 448
BfrI CTTAAG 1 cut(s) 793
BfuAI ACCTGC 1 cut(s) 930
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 6 cut(s) 22, 144, 291, 443, 570, 916
BlpI GCTNAGC 1 cut(s) 2172
BlsI GCNGC 6 cut(s) 23, 145, 292, 444, 571, 917
Bme1390I CCNGG 7 cut(s) 6, 44, 893, 944, 1787, 1947, 2106
Bme18I GGWCC 1 cut(s) 1867
BmeT110I CYCGRG 1 cut(s) 1700
BmgBI CACGTC 1 cut(s) 1583
BmgT120I GGNCC 2 cut(s) 317, 1867
BmiI GGNNCC 3 cut(s) 25, 588, 1869
BmrFI CCNGG 7 cut(s) 6, 44, 893, 944, 1787, 1947, 2106
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 7 cut(s) 149, 235, 502, 1822, 1837, 1859, 2218
BmuI ACTGGG 1 cut(s) 274
BoxI GACNNNNGTC 1 cut(s) 1581
BpiI GAAGAC 1 cut(s) 2066
BpmI CTGGAG 4 cut(s) 65, 258, 1344, 1929
Bpu1102I GCTNAGC 1 cut(s) 2172
BpuEI CTTGAG 4 cut(s) 803, 1403, 1751, 2048
BpuMI CCSGG 1 cut(s) 944
BsaBI GATNNNNATC 1 cut(s) 2367
BsaJI CCNNGG 4 cut(s) 497, 867, 1151, 2104
BsaWI WCCGGW 2 cut(s) 700, 803
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 238, 700, 1156, 1322, 1771, 2105, 2250
Bse1I ACTGG 4 cut(s) 280, 1448, 1548, 1599
Bse3DI GCAATG 2 cut(s) 633, 879
Bse8I GATNNNNATC 1 cut(s) 2367
BseBI CCWGG 6 cut(s) 6, 44, 893, 1787, 1947, 2106
BseDI CCNNGG 4 cut(s) 497, 867, 1151, 2104
BseGI GGATG 6 cut(s) 76, 164, 493, 1002, 1579, 1759
BseJI GATNNNNATC 1 cut(s) 2367
BseLI CCNNNNNNNGG 8 cut(s) 190, 238, 700, 1156, 1322, 1771, 2105, 2250
BseMI GCAATG 2 cut(s) 633, 879
BseMII CTCAG 5 cut(s) 746, 1850, 1978, 2031, 2163
BseNI ACTGG 4 cut(s) 280, 1448, 1548, 1599
BseRI GAGGAG 2 cut(s) 1299, 1728
BseSI GKGCMC 3 cut(s) 1409, 1991, 2244
BseXI GCAGC 3 cut(s) 454, 581, 902
BseYI CCCAGC 1 cut(s) 208
BshFI GGCC 7 cut(s) 83, 143, 319, 891, 1165, 1209, 1828
BsiHKAI GWGCWC 5 cut(s) 51, 1071, 1409, 1991, 2178
BsiHKCI CYCGRG 1 cut(s) 1700
BsiSI CCGG 3 cut(s) 701, 804, 944
BslFI GGGAC 3 cut(s) 1172, 1853, 2282
BslI CCNNNNNNNGG 8 cut(s) 190, 238, 700, 1156, 1322, 1771, 2105, 2250
BsmAI GTCTC 2 cut(s) 1195, 1320
BsmBI CGTCTC 1 cut(s) 1195
BsmFI GGGAC 3 cut(s) 1172, 1853, 2282
BsnI GGCC 7 cut(s) 83, 143, 319, 891, 1165, 1209, 1828
BsoBI CYCGRG 1 cut(s) 1700
Bsp1286I GDGCHC 6 cut(s) 51, 1071, 1409, 1991, 2178, 2244
Bsp1720I GCTNAGC 1 cut(s) 2172
Bsp19I CCATGG 1 cut(s) 497
BspACI CCGC 6 cut(s) 21, 144, 290, 823, 925, 1316
BspANI GGCC 7 cut(s) 83, 143, 319, 891, 1165, 1209, 1828
BspCNI CTCAG 5 cut(s) 747, 1849, 1977, 2032, 2164
BspLI GGNNCC 3 cut(s) 25, 588, 1869
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 930
BspPI GGATC 5 cut(s) 500, 754, 1815, 2042, 2108
BspTI CTTAAG 1 cut(s) 793
BsrDI GCAATG 2 cut(s) 633, 879
BsrI ACTGG 4 cut(s) 280, 1448, 1548, 1599
BssECI CCNNGG 4 cut(s) 497, 867, 1151, 2104
BssNAI GTATAC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 137
BssT1I CCWWGG 3 cut(s) 497, 867, 1151
Bst1107I GTATAC 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 6 cut(s) 6, 44, 893, 1787, 1947, 2106
Bst4CI ACNGT 8 cut(s) 172, 193, 400, 1516, 1885, 2023, 2151, 2350
Bst6I CTCTTC 2 cut(s) 411, 482
BstAFI CTTAAG 1 cut(s) 793
BstAPI GCANNNNNTGC 2 cut(s) 1252, 1637
BstC8I GCNNGC 5 cut(s) 612, 1320, 1455, 1941, 2190
BstDEI CTNAG 7 cut(s) 335, 755, 1235, 1836, 1964, 2040, 2172
BstDSI CCRYGG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 2103
BstF5I GGATG 6 cut(s) 76, 164, 493, 1002, 1579, 1759
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 1 cut(s) 447
BstMAI GTCTC 2 cut(s) 1195, 1320
BstMWI GCNNNNNNNGC 2 cut(s) 1252, 1637
BstNI CCWGG 6 cut(s) 6, 44, 893, 1787, 1947, 2106
BstNSI RCATGY 4 cut(s) 357, 482, 1255, 1322
BstPAI GACNNNNGTC 1 cut(s) 1581
BstSCI CCNGG 7 cut(s) 4, 42, 891, 942, 1785, 1945, 2104
BstSFI CTRYAG 3 cut(s) 312, 448, 567
BstSLI GKGCMC 3 cut(s) 1409, 1991, 2244
BstV1I GCAGC 3 cut(s) 454, 581, 902
BstV2I GAAGAC 1 cut(s) 2066
BstX2I RGATCY 3 cut(s) 746, 1045, 1807
BstXI CCANNNNNNTGG 1 cut(s) 1482
BstYI RGATCY 3 cut(s) 746, 1045, 1807
BstZ17I GTATAC 1 cut(s) 189
BsuRI GGCC 7 cut(s) 83, 143, 319, 891, 1165, 1209, 1828
BtgI CCRYGG 1 cut(s) 497
BtgZI GCGATG 2 cut(s) 79, 212
BtrI CACGTC 1 cut(s) 1583
BtsCI GGATG 6 cut(s) 76, 164, 493, 1002, 1579, 1759
BtsI GCAGTG 4 cut(s) 123, 1068, 1363, 2354
BtsIMutI CAGTG 5 cut(s) 123, 1068, 1363, 1592, 2354
BveI ACCTGC 1 cut(s) 930
Cac8I GCNNGC 5 cut(s) 612, 1320, 1455, 1941, 2190
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 1 cut(s) 447
Cfr13I GGNCC 2 cut(s) 317, 1867
Csp6I GTAC 2 cut(s) 1126, 2346
CviAII CATG 7 cut(s) 354, 479, 498, 545, 863, 1252, 1319
CviQI GTAC 2 cut(s) 1126, 2346
DdeI CTNAG 7 cut(s) 335, 755, 1235, 1836, 1964, 2040, 2172
DraIII CACNNNGTG 1 cut(s) 1430
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 411, 482
EarI CTCTTC 2 cut(s) 411, 482
Ecl136II GAGCTC 3 cut(s) 49, 1069, 2176
Eco130I CCWWGG 3 cut(s) 497, 867, 1151
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 3 cut(s) 51, 1071, 2178
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 1 cut(s) 1867
Eco47III AGCGCT 1 cut(s) 446
Eco53kI GAGCTC 3 cut(s) 49, 1069, 2176
Eco57I CTGAAG 2 cut(s) 684, 711
Eco88I CYCGRG 1 cut(s) 1700
EcoICRI GAGCTC 3 cut(s) 49, 1069, 2176
EcoNI CCTNNNNNAGG 1 cut(s) 2103
EcoO109I RGGNCCY 1 cut(s) 1867
EcoRII CCWGG 6 cut(s) 4, 42, 891, 1785, 1945, 2104
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 3 cut(s) 497, 867, 1151
EcoT22I ATGCAT 2 cut(s) 1852, 2233
EcoT38I GRGCYC 3 cut(s) 51, 1071, 2178
ErhI CCWWGG 3 cut(s) 497, 867, 1151
Esp3I CGTCTC 1 cut(s) 1195
FaeI CATG 7 cut(s) 357, 482, 501, 548, 866, 1255, 1322
FalI AAGNNNNNCTT 6 cut(s) 657, 689, 1218, 1250, 1856, 1888
FaqI GGGAC 3 cut(s) 1172, 1853, 2282
FatI CATG 7 cut(s) 353, 478, 497, 544, 862, 1251, 1318
FauI CCCGC 2 cut(s) 830, 1323
FauNDI CATATG 2 cut(s) 1477, 2233
FbaI TGATCA 2 cut(s) 970, 1096
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 6 cut(s) 22, 144, 291, 443, 570, 916
FokI GGATG 6 cut(s) 83, 171, 480, 1009, 1586, 1746
FriOI GRGCYC 3 cut(s) 51, 1071, 2178
Fsp4HI GCNGC 6 cut(s) 22, 144, 291, 443, 570, 916
FspBI CTAG 3 cut(s) 1029, 1665, 1811
GlaI GCGC 1 cut(s) 446
GluI GCNGC 6 cut(s) 22, 144, 291, 443, 570, 916
GsaI CCCAGC 1 cut(s) 212
GsuI CTGGAG 4 cut(s) 65, 258, 1344, 1929
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 7 cut(s) 83, 143, 319, 891, 1165, 1209, 1828
HapII CCGG 3 cut(s) 701, 804, 944
HhaI GCGC 1 cut(s) 447
Hin1II CATG 7 cut(s) 357, 482, 501, 548, 866, 1255, 1322
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HinfI GANTC 7 cut(s) 1175, 1376, 1659, 1919, 2164, 2315, 2320
HpaII CCGG 3 cut(s) 701, 804, 944
HphI GGTGA 4 cut(s) 476, 569, 1304, 1558
Hpy166II GTNNAC 5 cut(s) 189, 1305, 1407, 1604, 1989
Hpy188I TCNGA 9 cut(s) 28, 664, 756, 1104, 1839, 1903, 2135, 2161, 2199
Hpy8I GTNNAC 5 cut(s) 189, 1305, 1407, 1604, 1989
Hpy99I CGWCG 1 cut(s) 1611
HpyAV CCTTC 5 cut(s) 52, 230, 1071, 1214, 2109
HpyCH4III ACNGT 8 cut(s) 172, 193, 400, 1516, 1885, 2023, 2151, 2350
HpyCH4IV ACGT 3 cut(s) 236, 1582, 1609
HpyF10VI GCNNNNNNNGC 2 cut(s) 1252, 1637
HpyF3I CTNAG 7 cut(s) 335, 755, 1235, 1836, 1964, 2040, 2172
HpySE526I ACGT 3 cut(s) 236, 1582, 1609
Hsp92II CATG 7 cut(s) 357, 482, 501, 548, 866, 1255, 1322
HspAI GCGC 1 cut(s) 445
Ksp22I TGATCA 2 cut(s) 970, 1096
LmnI GCTCC 6 cut(s) 29, 46, 439, 1066, 1741, 1948
Lsp1109I GCAGC 3 cut(s) 454, 581, 902
LweI GCATC 7 cut(s) 149, 235, 502, 1822, 1837, 1859, 2218
MaeI CTAG 3 cut(s) 1029, 1665, 1811
MaeII ACGT 3 cut(s) 236, 1582, 1609
MboII GAAGA 8 cut(s) 49, 274, 395, 428, 499, 677, 1229, 2071
MfeI CAATTG 1 cut(s) 1167
MflI RGATCY 3 cut(s) 746, 1045, 1807
MhlI GDGCHC 6 cut(s) 51, 1071, 1409, 1991, 2178, 2244
MlyI GAGTC 1 cut(s) 2314
MmeI TCCRAC 2 cut(s) 48, 983
Mph1103I ATGCAT 2 cut(s) 1852, 2233
MroXI GAANNNNTTC 3 cut(s) 585, 669, 2022
MslI CAYNNNNRTG 6 cut(s) 795, 1089, 1317, 1480, 1517, 2376
MspCI CTTAAG 1 cut(s) 793
MspI CCGG 3 cut(s) 701, 804, 944
MspR9I CCNGG 7 cut(s) 6, 44, 893, 944, 1787, 1947, 2106
MunI CAATTG 1 cut(s) 1167
MvaI CCWGG 6 cut(s) 6, 44, 893, 1787, 1947, 2106
MwoI GCNNNNNNNGC 2 cut(s) 1252, 1637
NciI CCSGG 1 cut(s) 944
NcoI CCATGG 1 cut(s) 497
NdeI CATATG 2 cut(s) 1477, 2233
NlaIII CATG 7 cut(s) 357, 482, 501, 548, 866, 1255, 1322
NlaIV GGNNCC 3 cut(s) 25, 588, 1869
NmuCI GTSAC 5 cut(s) 109, 799, 1082, 1546, 2350
NsiI ATGCAT 2 cut(s) 1852, 2233
NspI RCATGY 4 cut(s) 357, 482, 1255, 1322
OliI CACNNNNGTG 1 cut(s) 795
PaeI GCATGC 1 cut(s) 1322
PaeR7I CTCGAG 1 cut(s) 1700
PceI AGGCCT 1 cut(s) 1209
PciI ACATGT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PdmI GAANNNNTTC 3 cut(s) 585, 669, 2022
PfeI GAWTC 6 cut(s) 1175, 1376, 1659, 1919, 2164, 2315
PfoI TCCNGGA 1 cut(s) 1945
PkrI GCNGC 6 cut(s) 23, 145, 292, 444, 571, 917
PleI GAGTC 1 cut(s) 2314
PpsI GAGTC 1 cut(s) 2314
PpuMI RGGWCCY 1 cut(s) 1867
PscI ACATGT 1 cut(s) 478
PshAI GACNNNNGTC 1 cut(s) 1581
Psp124BI GAGCTC 3 cut(s) 51, 1071, 2178
Psp1406I AACGTT 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 1867
Psp6I CCWGG 6 cut(s) 4, 42, 891, 1785, 1945, 2104
PspFI CCCAGC 1 cut(s) 208
PspGI CCWGG 6 cut(s) 4, 42, 891, 1785, 1945, 2104
PspN4I GGNNCC 3 cut(s) 25, 588, 1869
PspPI GGNCC 2 cut(s) 317, 1867
PspPPI RGGWCCY 1 cut(s) 1867
PspXI VCTCGAGB 1 cut(s) 1700
PstI CTGCAG 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 3 cut(s) 746, 1045, 1807
RsaI GTAC 2 cut(s) 1127, 2347
RsaNI GTAC 2 cut(s) 1126, 2346
RseI CAYNNNNRTG 6 cut(s) 795, 1089, 1317, 1480, 1517, 2376
SacI GAGCTC 3 cut(s) 51, 1071, 2178
SatI GCNGC 6 cut(s) 22, 144, 291, 443, 570, 916
Sau96I GGNCC 2 cut(s) 317, 1867
SchI GAGTC 1 cut(s) 2314
ScrFI CCNGG 7 cut(s) 6, 44, 893, 944, 1787, 1947, 2106
SduI GDGCHC 6 cut(s) 51, 1071, 1409, 1991, 2178, 2244
SfaNI GCATC 7 cut(s) 149, 235, 502, 1822, 1837, 1859, 2218
SfcI CTRYAG 3 cut(s) 312, 448, 567
Sfr274I CTCGAG 1 cut(s) 1700
SinI GGWCC 1 cut(s) 1867
SlaI CTCGAG 1 cut(s) 1700
SmiMI CAYNNNNRTG 6 cut(s) 795, 1089, 1317, 1480, 1517, 2376
SmlI CTYRAG 6 cut(s) 782, 793, 1382, 1700, 1766, 2027
SmoI CTYRAG 6 cut(s) 782, 793, 1382, 1700, 1766, 2027
SpeI ACTAGT 1 cut(s) 1028
SphI GCATGC 1 cut(s) 1322
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 6 cut(s) 21, 144, 290, 823, 925, 1316
SspI AATATT 1 cut(s) 712
SspMI CTAG 3 cut(s) 1029, 1665, 1811
SstI GAGCTC 3 cut(s) 51, 1071, 2178
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 7 cut(s) 4, 42, 891, 942, 1785, 1945, 2104
StyI CCWWGG 3 cut(s) 497, 867, 1151
TaaI ACNGT 8 cut(s) 172, 193, 400, 1516, 1885, 2023, 2151, 2350
TaiI ACGT 3 cut(s) 239, 1585, 1612
TaqI TCGA 7 cut(s) 166, 260, 1540, 1701, 2178, 2307, 2318
TatI WGTACW 1 cut(s) 1125
TauI GCSGC 3 cut(s) 24, 146, 293
TfiI GAWTC 6 cut(s) 1175, 1376, 1659, 1919, 2164, 2315
TscAI CASTG 5 cut(s) 130, 1068, 1370, 1599, 2361
TseFI GTSAC 5 cut(s) 109, 799, 1082, 1546, 2350
TseI GCWGC 3 cut(s) 442, 569, 915
Tsp45I GTSAC 5 cut(s) 109, 799, 1082, 1546, 2350
TspDTI ATGAA 7 cut(s) 288, 517, 549, 1157, 1256, 1425, 2072
TspRI CASTG 5 cut(s) 130, 1068, 1370, 1599, 2361
Vha464I CTTAAG 1 cut(s) 793
VneI GTGCAC 2 cut(s) 1405, 1987
VpaK11BI GGWCC 1 cut(s) 1867
XagI CCTNNNNNAGG 1 cut(s) 2103
XapI RAATTY 4 cut(s) 325, 341, 1415, 1464
XbaI TCTAGA 1 cut(s) 1810
XceI RCATGY 4 cut(s) 357, 482, 1255, 1322
XhoI CTCGAG 1 cut(s) 1700
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 3 cut(s) 585, 669, 2022
XspI CTAG 3 cut(s) 1029, 1665, 1811
Zsp2I ATGCAT 2 cut(s) 1852, 2233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.