RchiOBHm_Chr1g0324801

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000872
Physical Location & Seq
Forward (+)
12561716 .. 12562513
798 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55462

Sequence Viewer

Length: 798 bp
ATGACACTAGCTGGTTTACGACGTAGGGGCGTGACATCGACTGCTATTAATACTTTTGTTAGAGAAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATATGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCCGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCAGAGTCCTACAAGGTTCCAATTTCCATGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCTCTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAAGATGTTGTTTTAGCCGAGGATAATGAGACTATCCTTGAGATAAGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAGGGGGGTGCTGCATTGGGTTGCTGAACCTTCTCCGGGGATCAATCCACTAGAAGTGGAAGTCCGATTGTTTGATAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCAAAAGTGGTAATACCAATTGCATTCGCTGTTCCTCAACTTGCAAAAGGTGTTGATGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCCTGAGAAGCTTGTGTTTAATCGTACGGTCACACTGCGAGATAGCTACAAGAATGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

265

Amino Acids

30.22

Weight (kDa)

6.37

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c_C PF03950 50 - 153 6.9e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 163 - 241 1.7e-16 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 720
AccIII TCCGGA 1 cut(s) 460
AclWI GGATC 3 cut(s) 167, 452, 527
AfaI GTAC 3 cut(s) 119, 290, 760
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 217, 515
AgsI TTSAA 1 cut(s) 565
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 6 cut(s) 11, 136, 149, 586, 745, 780
AluI AGCT 6 cut(s) 11, 136, 149, 586, 745, 780
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 3 cut(s) 167, 452, 527
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 460
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 490
AseI ATTAAT 1 cut(s) 48
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 240
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 2 cut(s) 67, 692
AsuII TTCGAA 1 cut(s) 696
AvaI CYCGRG 1 cut(s) 113
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 477
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 8, 530
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 1 cut(s) 491
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 1 cut(s) 492
Bme1390I CCNGG 2 cut(s) 348, 516
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 2 cut(s) 348, 516
BmsI GCATC 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 476
BplI GAGNNNNNCTC 2 cut(s) 120, 152
Bpu1102I GCTNAGC 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 516
BsaJI CCNNGG 4 cut(s) 279, 417, 479, 515
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 515
Bse1I ACTGG 2 cut(s) 203, 599
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 4 cut(s) 279, 417, 479, 515
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 2 cut(s) 217, 515
BseMII CTCAG 5 cut(s) 240, 379, 573, 582, 729
BseNI ACTGG 2 cut(s) 203, 599
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 477
BshFI GGCC 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 461, 515
BsiWI CGTACG 1 cut(s) 758
BslI CCNNNNNNNGG 2 cut(s) 217, 515
BsmAI GTCTC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 460
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 4 cut(s) 172, 457, 519, 604
Bsp1720I GCTNAGC 1 cut(s) 582
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 5 cut(s) 241, 378, 574, 581, 730
BspEI TCCGGA 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 3 cut(s) 167, 452, 527
BspQI GCTCTTC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 696
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 2 cut(s) 203, 599
BssECI CCNNGG 4 cut(s) 279, 417, 479, 515
BssMI GATC 4 cut(s) 172, 457, 519, 604
BssT1I CCWWGG 2 cut(s) 279, 479
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 224, 763
Bst6I CTCTTC 1 cut(s) 126
BstAUI TGTACA 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 696
BstDEI CTNAG 5 cut(s) 249, 365, 568, 582, 738
BstDSI CCRYGG 1 cut(s) 279
BstENI CCTNNNNNAGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 4 cut(s) 175, 460, 522, 607
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 4 cut(s) 172, 457, 519, 604
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 2 cut(s) 346, 514
BstV1I GCAGC 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 1 cut(s) 767
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 240
Csp6I GTAC 3 cut(s) 118, 289, 759
CviAII CATG 2 cut(s) 280, 375
CviQI GTAC 3 cut(s) 118, 289, 759
DdeI CTNAG 5 cut(s) 249, 365, 568, 582, 738
DpnI GATC 4 cut(s) 174, 459, 521, 606
DpnII GATC 4 cut(s) 172, 457, 519, 604
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 279, 479
Eco24I GRGCYC 1 cut(s) 138
Eco47III AGCGCT 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 2 cut(s) 279, 479
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 2 cut(s) 279, 479
FaeI CATG 2 cut(s) 283, 378
FaiI YATR 7 cut(s) 95, 102, 104, 215, 281, 376, 456
FatI CATG 2 cut(s) 279, 374
FauNDI CATATG 1 cut(s) 102
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 1 cut(s) 491
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 491
FspBI CTAG 2 cut(s) 8, 530
GlaI GCGC 1 cut(s) 299
GluI GCNGC 1 cut(s) 491
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 1 cut(s) 241
HapII CCGG 2 cut(s) 461, 515
HhaI GCGC 1 cut(s) 300
Hin1II CATG 2 cut(s) 283, 378
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 9 cut(s) 72, 97, 106, 245, 257, 320, 574, 728, 733
HpaII CCGG 2 cut(s) 461, 515
HphI GGTGA 2 cut(s) 67, 692
Hpy166II GTNNAC 2 cut(s) 17, 721
Hpy188I TCNGA 9 cut(s) 128, 250, 304, 338, 368, 545, 571, 604, 609
Hpy188III TCNNGA 2 cut(s) 461, 737
Hpy8I GTNNAC 2 cut(s) 17, 721
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 2 cut(s) 224, 763
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 4 cut(s) 398, 493, 641, 662
HpyF3I CTNAG 5 cut(s) 249, 365, 568, 582, 738
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 2 cut(s) 283, 378
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 4 cut(s) 172, 457, 519, 604
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 3 cut(s) 154, 305, 349
LpnPI CCDG 9 cut(s) 91, 184, 333, 360, 474, 528, 580, 591, 750
Lsp1109I GCAGC 1 cut(s) 477
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 8, 530
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 3 cut(s) 31, 680, 763
MalI GATC 4 cut(s) 174, 459, 521, 606
MboI GATC 4 cut(s) 172, 457, 519, 604
MboII GAAGA 3 cut(s) 143, 478, 481
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 66, 273, 636
MlyI GAGTC 4 cut(s) 115, 266, 314, 727
MnlI CCTC 6 cut(s) 122, 162, 221, 244, 412, 663
MroI TCCGGA 1 cut(s) 460
MseI TTAA 3 cut(s) 48, 315, 753
MspI CCGG 2 cut(s) 461, 515
MspR9I CCNGG 2 cut(s) 348, 516
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 348
NciI CCSGG 1 cut(s) 516
NcoI CCATGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 102
NdeII GATC 4 cut(s) 172, 457, 519, 604
NlaIII CATG 2 cut(s) 283, 378
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 3 cut(s) 31, 680, 763
NspI RCATGY 1 cut(s) 378
NspV TTCGAA 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 126
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 5 cut(s) 72, 97, 245, 574, 728
Pfl23II CGTACG 1 cut(s) 758
PfoI TCCNGGA 1 cut(s) 346
PkrI GCNGC 1 cut(s) 492
PleI GAGTC 4 cut(s) 114, 265, 314, 727
PpsI GAGTC 4 cut(s) 114, 265, 314, 727
PshBI ATTAAT 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspLI CGTACG 1 cut(s) 758
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 240
PspXI VCTCGAGB 1 cut(s) 113
RsaI GTAC 3 cut(s) 119, 290, 760
RsaNI GTAC 3 cut(s) 118, 289, 759
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 719
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 48, 315, 753
SatI GCNGC 1 cut(s) 491
Sau3AI GATC 4 cut(s) 172, 457, 519, 604
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 4 cut(s) 115, 266, 314, 727
ScrFI CCNGG 2 cut(s) 348, 516
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 1 cut(s) 385
Sfr274I CTCGAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 696
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 2 cut(s) 113, 437
SmoI CTYRAG 2 cut(s) 113, 437
Sse9I AATT 3 cut(s) 66, 273, 636
SspMI CTAG 2 cut(s) 8, 530
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 346, 514
StyI CCWWGG 2 cut(s) 279, 479
TaaI ACNGT 2 cut(s) 224, 763
TaiI ACGT 1 cut(s) 25
TaqI TCGA 6 cut(s) 38, 114, 450, 696, 720, 731
TasI AATT 3 cut(s) 66, 273, 636
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 5 cut(s) 72, 97, 245, 574, 728
Tru1I TTAA 3 cut(s) 48, 315, 753
Tru9I TTAA 3 cut(s) 48, 315, 753
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 3 cut(s) 31, 680, 763
TseI GCWGC 1 cut(s) 490
Tsp45I GTSAC 3 cut(s) 31, 680, 763
TspDTI ATGAA 1 cut(s) 89
TspRI CASTG 1 cut(s) 774
VspI ATTAAT 1 cut(s) 48
XagI CCTNNNNNAGG 1 cut(s) 215
XceI RCATGY 1 cut(s) 378
XhoI CTCGAG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 2 cut(s) 8, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.